Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| selection of nectar plants for use in ecological engineering to promote biological control of rice pests by the predatory bug, cyrtorhinus lividipennis, (heteroptera: miridae). | ecological engineering for pest management involves the identification of optimal forms of botanical diversity to incorporate into a farming system to suppress pests, by promoting their natural enemies. whilst this approach has been extensively researched in many temperate crop systems, much less has been done for rice. this paper reports the influence of various plant species on the performance of a key natural enemy of rice planthopper pests, the predatory mirid bug, cyrtorhinus lividipennis. ... | 2014 | 25254377 |
| contrasting effects of ethylene biosynthesis on induced plant resistance against a chewing and a piercing-sucking herbivore in rice. | ethylene is a stress hormone with contrasting effects on herbivore resistance. however, it remains unknown whether these differences are plant- or herbivore-specific. we cloned a rice 1-aminocyclopropane-1-carboxylic acid (acc) synthase gene, osacs2, whose transcripts were rapidly up-regulated in response to mechanical wounding and infestation by two important pests: the striped stem borer (ssb) chilo suppressalis and the brown planthopper (bph) nilaparvata lugens. antisense expression of osacs2 ... | 2014 | 25064847 |
| the 9-lipoxygenase osr9-lox1 interacts with the 13-lipoxygenase-mediated pathway to regulate resistance to chewing and piercing-sucking herbivores in rice. | oxylipins produced by the 13-lipoxygenase (lox) have been reported to play an important role in plant defense responses to herbivores. yet, the role of oxylipins produced by the 9-lox pathway in this process remains largely unknown. here we cloned a gene encoding a chloroplast-localized 9-lox, osr9-lox1, from rice. transcriptional analysis revealed that herbivore infestation, mechanical wounding and jasmonic acid (ja) treatment either repressed or did not enhance the level of osr9-lox1 transcrip ... | 2014 | 24410960 |
| osmpk3 is a tey-type rice mapk in group c and phosphorylates osbhlh65, a transcription factor binding to the e-box element. | osmpk3 is a tey-type rice mapk belonging to group c and directly phosphorylates osbhlh65 in the nucleus. osmpk3 and osbhlh65 are induced by biotic stress and defense-related hormones. mitogen-activated protein kinases (mapks) are involved in the majority of signaling pathways that regulate plant development and stress tolerance via the phosphorylation of target molecules. plant mapks are classified into two subtypes, tey and tdy, according to the txy (x = e or d) motif in their activation loop, ... | 2014 | 24777821 |
| susceptibility of sogatella furcifera and laodelphax striatellus (hemiptera: delphacidae) to six insecticides in china. | the whitebacked planthopper, sogatella furcifera (horváth), and small brown planthopper, laodelphax striatellus (fallén), both are important crop pests throughout china, especially in rice. application of chemical insecticides is the major control practice. consequently, insecticide resistance has become an urgent issue. in this study, resistance levels to six conventional insecticides were evaluated for these two species collected from major occurring areas of china. additionally, imidacloprid- ... | 2014 | 26309282 |
| an alternative suite of universal primers for genotyping in multiplex pcr. | the universal primer three-primer approach can dramatically reduce the cost when genotyping the microsatellites. one former research reported four universal primers that can be used in singleplex and multiplex genotyping. in this study, we proposed an alternative suite of universal primers with four dyes for genotyping 8-12 loci in one single run. this multiplex method was tested on tetranychus truncatus. published microsatellite loci of t. kanzawai, frankliniella occidentalis and nilaparvata lu ... | 2014 | 24658225 |
| bt rice expressing cry2aa does not harm cyrtorhinus lividipennis, a main predator of the nontarget herbivore nilapavarta lugens. | t2a-1 is a newly developed transgenic rice that expresses a synthesized cry2aa gene driven by the maize ubiquitin promoter. t2a-1 exhibits high resistance against lepidopteran pests of rice. the brown planthopper, nilapavarta lugens (stål), is a main nontarget sap-sucking insect pest of rice, and cyrtorhinus lividipennis (reuter) is the major predator of the eggs and young nymphs of planthoppers. as c. lividipennis may expose to the cry2aa protein via n. lugens, it is therefore essential to asse ... | 2014 | 25375147 |
| bac and rna sequencing reveal the brown planthopper resistance gene bph15 in a recombination cold spot that mediates a unique defense mechanism. | brown planthopper (bph, nilaparvata lugens stål), is the most destructive phloem-feeding insect pest of rice (oryza sativa). the bph-resistance gene bph15 has been proved to be effective in controlling the pest and widely applied in rice breeding programs. nevertheless, molecular mechanism of the resistance remain unclear. in this study, we narrowed down the position of bph15 on chromosome 4 and investigated the transcriptome of bph15 rice after bph attacked. | 2014 | 25109872 |
| map-based cloning and characterization of a brown planthopper resistance gene bph26 from oryza sativa l. ssp. indica cultivar adr52. | the brown planthopper (bph) is the most serious insect pest of rice in asia. the indica rice cultivar adr52 carries two bph resistance genes, bph26 (brown planthopper resistance 26) and bph25. map-based cloning of bph26 revealed that bph26 encodes a coiled-coil-nucleotide-binding-site-leucine-rich repeat (cc-nbs-lrr) protein. bph26 mediated sucking inhibition in the phloem sieve element. bph26 was identical to bph2 on the basis of dna sequence analysis and feeding ability of the bph2-virulent bi ... | 2014 | 25076167 |
| genome-wide mapping of virulence in brown planthopper identifies loci that break down host plant resistance. | insects and plants have coexisted for over 350 million years and their interactions have affected ecosystems and agricultural practices worldwide. variation in herbivorous insects' virulence to circumvent host resistance has been extensively documented. however, despite decades of investigation, the genetic foundations of virulence are currently unknown. the brown planthopper (nilaparvata lugens) is the most destructive rice (oryza sativa) pest in the world. the identification of the resistance ... | 2014 | 24911169 |
| immunodetection of a brown planthopper (nilaparvata lugens stål) salivary catalase-like protein into tissues of rice, oryza sativa. | saliva plays an important role in host plant-phloem-feeding insect molecular interactions. to better elucidate the role of insect saliva, a series of experiments were conducted to establish if catalase from the salivary glands of the brown planthopper (bph; nilaparvata lugens stål) was secreted into rice host plant tissue during feeding. catalase is the main enzyme that decomposes hydrogen peroxide (h2o2) at high concentrations. h2o2 is a part of the free radicals system that mediates important ... | 2014 | 24164290 |
| the complete mitochondrial genome sequence of sogatella furcifera (horváth) and a comparative mitogenomic analysis of three predominant rice planthoppers. | the white-backed planthopper (wbph), sogatella furcifera (horváth), is one of the most destructive pests of rice crops in many asian countries. using long-pcr and shotgun library methods, we sequenced the entire mitochondrial genomes (mt-genomes) of two wbph individuals. total lengths of the mt-genome of the two wbph individuals were 16,612 bp and 16,654 bp with an identical at content of 76.19%. among the 13 protein coding genes (pcgs), only nad5 used an atypical initiation codon gtg. most of t ... | 2014 | 24120898 |
| biochemical and molecular characterisation and cross-resistance in field and laboratory chlorpyrifos-resistant strains of laodelphax striatellus (hemiptera: delphacidae) from eastern china. | laboratory selection is often employed in resistance mechanism studies because field-derived populations commonly do not have high enough resistance for such studies. in the present study, a field-collected laodelphax striatellus population from eastern china was laboratory selected for chlorpyrifos resistance and susceptibility, and the developed strains, along with a field population, were studied for cross-resistance and resistance mechanisms at biochemical and molecular levels. | 2014 | 24115461 |
| small brown planthopper resistance loci in wild rice (oryza officinalis). | host-plant resistance is the most practical and economical approach to control the rice planthoppers. however, up to date, few rice germplasm accessions that are resistant to the all three kinds of planthoppers (1) brown planthopper (bph; nilaparvata lugens stål), (2) the small brown planthopper (sbph; laodelphax striatellus fallen), and (3) the whitebacked planthopper (wbph, sogatella furcifera horvath) have been identified; consequently, the genetic basis for host-plant broad spectrum resistan ... | 2014 | 24504629 |
| effects of two pesticides, tzp and jgm, on reproduction of three planthopper species, nilaparvata lugens stål, sogatella furcifera horvath, and laodelphax striatella fallén. | three planthopper species, the brown planthopper (bph), nilaparvata lugens stål, the white-backed planthopper (wbph), sogatella furcifera horvath, and the small brown planthopper (sbph), laodelphax striatella fallén, often co-occur in rice grown regions of china. the present investigation examined effects of two pesticides, triazophos (tzp) and jinggangmycin (jgm) (a fungicide), on reproduction of bph, wbph, and sbph. the results showed that tzp stimulated the fecundity of the three planthopper ... | 2014 | 25307466 |
| identification and characterization of micrornas in small brown planthopper (laodephax striatellus) by next-generation sequencing. | micrornas (mirnas) are endogenous non-coding small rnas that regulate gene expression at the post-transcriptional level and are thought to play critical roles in many metabolic activities in eukaryotes. the small brown planthopper (laodephax striatellus fallén), one of the most destructive agricultural pests, causes great damage to crops including rice, wheat, and maize. however, information about the genome of l. striatellus is limited. in this study, a small rna library was constructed from a ... | 2014 | 25057821 |
| bio-effects of near-zero magnetic fields on the growth, development and reproduction of small brown planthopper, laodelphax striatellus and brown planthopper, nilaparvata lugens. | magnetic fields markedly affect the growth and development of many species of organisms potentially due to cryptochrome and endogenous presence of magnetic materials. sensitivity to magnetic fields can also be involved in geomagnetic orientation by some long-distance migratory insects. in this study, near-zero magnetic fields (nzmf) in relation to normal geomagnetic fields (gmf) were setup using the hypomagnetic field space system (hmfs) to investigate the effects of magnetic fields on the growt ... | 2014 | 24995837 |
| cis-12-oxo-phytodienoic acid stimulates rice defense response to a piercing-sucking insect. | the brown planthopper (bph, nilaparvata lugens) is a destructive, monophagous, piercing-sucking insect pest of rice. previous studies indicated that jasmonic acid (ja) positively regulates rice defense against chewing insect pests but negatively regulates it against the piercing-sucking insect of bph. we here demonstrated that overexpression of allene oxide cyclase (aoc) but not opr3 (cis-12-oxo-phytodienoic acid (opda) reductase 3, an enzyme adjacent to aoc in the ja synthetic pathway) signific ... | 2014 | 25239066 |
| [ecological responses of brown planthopper, nilaparvata lugens, to triazophos and deltamethrin on transgenic bt rice lines and their non-bt parental rice]. | in this study, the brown planthopper (bph), nilaparvata lugens (hemiptera: delphacidae), cultured on bt rice lines (t1c-19 with cry1c gene and t2a-1 with cry2a gene) and their parental rice mh63, were treated with a series of concentrations of triazophos and deltamethrin when they developed up to the third instar for the evaluation of the effects of bt rice on bph resurgence induced by pesticides. the results indicated that bph nymphal duration decreased with the increasing concentration of delt ... | 2014 | 25796912 |
| estimation of the age and amount of brown rice plant hoppers based on bionic electronic nose use. | the brown rice plant hopper (brph), nilaparvata lugens (stal), is one of the most important insect pests affecting rice and causes serious damage to the yield and quality of rice plants in asia. this study used bionic electronic nose technology to sample brph volatiles, which vary in age and amount. principal component analysis (pca), linear discrimination analysis (lda), probabilistic neural network (pnn), bp neural network (bpnn) and loading analysis (loadings) techniques were used to analyze ... | 2014 | 25268913 |
| wingless gene cloning and its role in manipulating the wing dimorphism in the white-backed planthopper, sogatella furcifera. | wingless gene (wg) plays a fundamental role in regulating the segment polarity and wing imaginal discs of insects. the rice planthoppers have an obvious wing dimorphism, and the long- and short-winged forms exist normally in natural populations. however, the molecular characteristics and functions of wg in rice planthoppers are poorly understood, and the relationship between expression level of wg and wing dimorphism has not been clarified. | 2014 | 25266639 |
| radar observations of the seasonal migration of brown planthopper (nilaparvata lugens stål) in southern china. | the summer and autumn migrations of the brown planthopper (nilaparvata lugens) were observed in southern china with a millimetric scanning entomological radar and a searchlight trap supplemented with capture in field cages, field surveys, and dissections of females. nilaparvata lugens took off at dusk and dawn in summer, but in autumn there was sometimes only a dusk take-off. the variation of the area density of the radar targets indicated that flight durations were about 9-10 h. in summer, plan ... | 2014 | 25229712 |
| different expression profiles suggest functional differentiation among chemosensory proteins in nilaparvata lugens (hemiptera: delphacidae). | chemosensory proteins (csps) play various roles in insect physiology including olfaction and development. the brown planthopper, nilaparvata lugens stål, is one of the most notorious rice pests worldwide. the wing-from variation and annually long distance migration imply that olfaction would play a key role in n. lugens behavior. in this study, full-length cdnas of nine csps were cloned by the rapid amplification of cdna ends procedure, and their expression profiles were determined by the quanti ... | 2014 | 25527582 |
| the insect ecdysone receptor is a good potential target for rnai-based pest control. | rna interference (rnai) has great potential for use in insect pest control. however, some significant challenges must be overcome before rnai-based pest control can become a reality. one challenge is the proper selection of a good target gene for rnai. here, we report that the insect ecdysone receptor (ecr) is a good potential target for rnai-based pest control in the brown planthopper nilaparvata lugens, a serious insect pest of rice plants. we demonstrated that the use of a 360 bp fragment (nl ... | 2014 | 25516715 |
| effects of exogenous plant growth regulator abscisic acid-induced resistance in rice on the expression of vitellogenin mrna in nilaparvata lugens (hemiptera: delphacidae) adult females. | recent study showed that exogenous abscisic acid (aba) acts as a regulator of plant resistance. this study investigated average injury scale and callose contents of rice, and vitellogenin (nlvg) mrna expression in nilaparvata lugens (stål) (hemiptera: delphacidae) adult females after third instar nymphs fed on exogenous aba-treated susceptible [taichung native one (tn1)] and moderately resistant (ir42) rice cultivars. the results showed that exogenous aba significantly decreased average injury s ... | 2014 | 25502025 |
| decrease of insecticide resistance over generations without exposure to insecticides in nilaparvata lugens (hemipteran: delphacidae). | the brown planthopper, nilaparvata lugens (stål), is one of the most important insect pests on paddy rice in tropical and temperate asia. overuse and misuse of insecticides have resulted in the development of high resistance to many different insecticides in this pest. studies were conducted to evaluate the change of resistance level to four insecticides over 15 generations without any exposure to insecticides in brown planthopper. after 15 generations' rearing without exposure to insecticide, b ... | 2014 | 25195455 |
| comparisons of topical and spray applications of two pesticides, triazophos and jinggangmycin, on the protein content in the ovaries and fat bodies of the brown planthopper nilaparvata lugens stål (hemiptera:delphacidae). | the pesticide-induced stimulation of reproduction in pests is one of the most important mechanisms of pest resurgence. there have been numerous reports on the insecticide-induced stimulation of reproduction. however, the relationship between pesticide application method and pest resurgence (stimulation of reproduction) has received little attention. here, we studied the effect of two treatment methods, triazophos (tzp) and jinggangmycin (jgm), on the protein content in the ovaries and fat bodies ... | 2014 | 25175656 |
| isolation, characterization, and cross-transferability of microsatellite markers from the whitebacked planthopper (sogatella furcifera). | the whitebacked planthopper sogatella furcifera (horváth) (hemiptera: delphacidae) is one of the most harmful pests of rice. in this study, 18 polymorphic microsatellite markers were developed from s. furcifera genomic libraries using the fast isolation by amplified fragment length polymorphism of sequences containing repeat protocols. microsatellite polymorphism was investigated using 32 individuals from one natural population. these 18 simple sequence repeat markers showed a number of alleles ... | 2014 | 25158251 |
| identification of promoter polymorphisms in the cytochrome p450 cyp6ay1 linked with insecticide resistance in the brown planthopper, nilaparvata lugens. | imidacloprid resistance in the brown planthopper, nilaparvata lugens, is primarily the result of the over-expression of cytochrome p450 monooxygenases. here, a field-collected strain of n. lugens was shown to be highly resistant to both imidacloprid and buprofezin. insecticide exposure and quantitative real-time pcr revealed that its resistance was mainly associated with a cytochrome p450 gene, cyp6ay1. cyp6ay1 is known to metabolize imidacloprid but its effect on buprofezin is unclear. in the 5 ... | 2014 | 25124988 |
| physiological efects of paichongding applied to rice on the nilaparvata lugens (stå l), the brown planthopper. | nilaparvata lugens (stål) is a major rice pest in asia. paichongding is a novel neonicotinoid insecticide developed in 2008. the effects of this insecticide on the activity of detoxification enzymes of n. lugens and on rice resistance to the pest were examined in the laboratory. the results showed that paichongding could significantly decrease the acetylcholinesterase and gshs transferase activities of n. lugens. the variation tendency of mixed function oxidase (mfo) activity was similar with th ... | 2014 | 25042427 |
| chitin deacetylase family genes in the brown planthopper, nilaparvata lugens (hemiptera: delphacidae). | chitin deacetylases (cdas) are enzymes required for one of the pathways of chitin degradation, in which chitosan is produced by the deacetylation of chitin. bioinformatic investigations with genomic and transcriptomic databases identified four genes encoding cdas in nilaparvata lugens (nlcdas). phylogenetic analysis showed that insect cdas were clustered into five major groups. group i, iii and iv cdas are found in all insect species, whereas the pupa-specific group ii and gut-specific group v c ... | 2014 | 24989071 |
| genetic mapping of the rice resistance-breaking gene of the brown planthopper nilaparvata lugens. | host plant resistance has been widely used for controlling the major rice pest brown planthopper (bph, nilaparvata lugens). however, adaptation of the wild bph population to resistance limits the effective use of resistant rice varieties. quantitative trait locus (qtl) analysis was conducted to identify resistance-breaking genes against the anti-feeding mechanism mediated by the rice resistance gene bph1. qtl analysis in iso-female bph lines with single-nucleotide polymorphism (snp) markers dete ... | 2014 | 24870048 |
| heat-dependent fecundity enhancement observed in nilaparvata lugens (hemiptera: delphacidae) after treatment with triazophos. | the brown planthopper nilaparvata lugens (stål) (hemiptera: delphacidae) is a serious pest of rice crops in the temperate and tropical regions of asia and australia, and it is also a classic example of an insecticide-induced resurgent pest. brown planthopper outbreaks have been reported to be closely associated with pesticide application. previous studies have shown that the insecticide triazophos enhances thermal tolerance and fecundity in brown planthopper. however, the effects of triazophos a ... | 2014 | 24763100 |
| the content of amino acids in artificial diet influences the development and reproduction of brown planthopper, nilaparvata lugens (stål). | nitrogen availability from dietary protein has profound effects on the physiology and ecology of insect herbivores. the amount of amino acids consumed by nilaparvata lugens impacts its phenotypic characteristics and reproduction. in this work, we hypothesized that amino acids deficiency leads to physiological trade-offs between survival and reproduction. we investigated the effect of larval nutrition on larval period, wing dimorphism, egg production, ovarian development, lifespan, and stored nut ... | 2014 | 24753101 |
| heavy metal bioaccumulation and mobility from rice plants to nilaparvata lugens (homoptera: delphacidae) in china. | samples of soils, rice plants, and the adult, long-winged, brown planthoppers, nilaparvata lugens (stål) (homoptera: delphacidae), were collected from 18 sites of 9 regions in southern china. the concentrations of seven elements (cu, zn, as, mo, ag, cd, and pb) were measured using inductively coupled plasma mass spectrometry. heavy metal mobility and bioaccumulation were analyzed in the rice plant-n. lugens system. the concentrations of zn, as, cd, and pb in rice plants were positively correlate ... | 2014 | 24735989 |
| will climate change affect outbreak patterns of planthoppers in bangladesh? | recently, planthoppers outbreaks have intensified across asia resulting in heavy rice yield losses. the problem has been widely reported as being induced by insecticides while other factors such as global warming that could be potential drivers have been neglected. here, we speculate that global warming may increase outbreak risk of brown planthopper (nilaparvata lugens stål.). we present data that demonstrate the relationship between climate variables (air temperature and precipitation) and the ... | 2014 | 24618677 |
| outbreaks of the brown planthopper nilaparvata lugens (stål) in the yangtze river delta: immigration or local reproduction? | an effective control strategy for migratory pests is difficult to implement because the cause of infestation (i.e., immigration or local reproduction) is often not established. in particular, the outbreak mechanisms of the brown planthopper, nilaparvata lugens (stål), an insect causing massive losses in rice fields in the yangtze river delta in china, are frequently unclear. field surveys of n. lugens were performed in jiangsu and zhejiang provinces in 2008 to 2010 and related historical data fr ... | 2014 | 24558459 |
| selection and evaluation of potential reference genes for gene expression analysis in the brown planthopper, nilaparvata lugens (hemiptera: delphacidae) using reverse-transcription quantitative pcr. | the brown planthopper (bph), nilaparvata lugens (hemiptera, delphacidae), is one of the most important rice pests. abundant genetic studies on bph have been conducted using reverse-transcription quantitative real-time pcr (qrt-pcr). using qrt-pcr, the expression levels of target genes are calculated on the basis of endogenous controls. these genes need to be appropriately selected by experimentally assessing whether they are stably expressed under different conditions. however, such studies on p ... | 2014 | 24466124 |
| de novo analysis of the nilaparvata lugens (stål) antenna transcriptome and expression patterns of olfactory genes. | we sequenced the antenna transcriptome of the brown planthopper (bph), nilaparvata lugens (stål), a global rice pest, and performed transcriptome analysis on bph antenna. we obtained about 40million 90bp reads that were assembled into 75,874 unigenes with a mean size of 456bp. among the antenna transcripts, 32,856 (43%) showed significant similarity (e-value <1e(-5)) to known proteins in the ncbi database. gene ontology and kyoto encyclopedia of genes and genomes (kegg) analyses were used to cla ... | 2014 | 24440828 |
| cell culture of the rice brown planthopper, nilaparvata lugens stål (hemiptera: delphacidae). | the rice brown planthopper (bph), nilaparvata lugens (stål), is one of the most destructive insect pests of rice in asia. although resistant rice varieties can be effective in managing planthopper populations, bph has repeatedly been able to overcome resistant rice varieties. it is possible that bph adaptation to resistant rice varieties may be related to its endosymbionts. we investigated methods for culturing bph cells in order to study in-depth interactions between rice, bph, and its endosymb ... | 2014 | 24399256 |
| bioefficacy of piper ribesioides (piperaceae) extracts against nilaparvata lugens stal. (homoptera: delphacidae). | the extracts of stem and seeds of piper ribesioides (piperaceae) were prepared in hexane, dichloromethane, ethyl acetate and ethanol and evaluated for their efficacy against 3-day-old adult brown plant hoppers, nilaparvata lugens stal under laboratory conditions. the treatment was given topically and results suggest the dose dependent response related to exposure time. the ethyl acetate extract was the best with an lc50 = 738 ppm-, 24 h post-treatment. there was substantial increase in activity ... | 2014 | 26084101 |
| actin-mediated transovarial transmission of a yeastlike symbiont in the brown planthopper. | the brown planthopper nilaparvata lugens (hemiptera, delphacidae) harbors an obligate endosymbiont called the yeastlike symbiont (yls) in their abdominal fat body. yls, a filamentous ascomycete belonging to the family clavicipitaceae, does not spend any part of its life cycle outside the planthopper's body. the yls is transferred to the next generation via transovarial transmission; it enters the epithelial plug at the posterior end of the host female's ovariole and is transferred to her offspri ... | 2014 | 24315960 |
| multi-generational effects of rice harboring bph15 on brown planthopper, nilaparvata lugens. | the brown planthopper (bph), nilaparvata lugens, is one of the most devastating rice pests in asia. resistant cultivars are an effective way of managing bph. bph15 is a bph resistance gene and has been introgressed into rice variety minghui 63 (mh63). the multi-generational effects of rice line mh63::15 (harboring bph15) on bph were investigated and compared with its parental line mh63. | 2014 | 23589438 |
| biological and biochemical characterization of a red-eye mutant in nilaparvata lugens (hemiptera: delphacidae). | a red-eye colony was established in our laboratory in brown planthopper (bph), nilaparvata lugens (stål), a major rice pest in asia. except for the red-eye phenotype, no other differences were observed between the wild-type (brown eye) and the mutant-type (red eye) in external characters. genetic analysis revealed that the red-eye phenotype was controlled by a single autosomal recessive allele. biological studies found that egg production and egg viability in the red-eye mutant colony were not s ... | 2014 | 23955841 |
| complete genome sequences of two iflaviruses from the brown planthopper, nilaparvata lugens. | the complete genome sequences of two new iflaviruses (genus iflavirus, family iflaviridae) were determined. these viral sequences were first identified in rna-seq contig sequences of nilaparvata lugens in two distinct colonies: izumo and kagoshima. the accuracy of the contig sequences of the two viruses was verified by restriction enzyme digestion of rt-pcr products from viruliferous insects. rt-pcr of rna extracted from honeydews after viruliferous insect feeding detected the expected viral pro ... | 2014 | 24065216 |
| transcriptome analysis of neuropeptides and g-protein coupled receptors (gpcrs) for neuropeptides in the brown planthopper nilaparvata lugens. | the genes encoding neuropeptides, neurohormones and their putative g-protein coupled receptors were identified in the brown planthopper (bph), nilaparvata lugens (stål) by transcriptome analysis (rna-seq). forty-eight candidate genes were found to encode neuropeptides or peptide hormones. these include all known insect neuropeptides and neurohormones, with the exception of neuropeptide-like precursor 2 (nplp2) and trissin. the gene coding for prothoracicotropic hormone (ptth) was first identifie ... | 2014 | 23932938 |
| molecular cloning and characterization of a ryanodine receptor gene in brown planthopper (bph), nilaparvata lugens (stål). | ryanodine receptors (ryrs) are a distinct class of intracellular calcium (ca(2+)) release channel. the recent discovery of diamide insecticides has prompted studies on insect ryrs. however, information about the structure and function of insect ryrs is still limited. in this study, we isolated and characterized a full-length ryr cdna (named nlryr) from the brown planthopper, nilaparvata lugens (stål) (homoptera: delphacidae), a serious rice pest throughout asia. | 2014 | 23893901 |
| insecticide susceptibilities in populations of two rice planthoppers, nilaparvata lugens and sogatella furcifera, immigrating into japan in the period 2005-2012. | the brown planthopper nilaparvata lugens and the whitebacked planthopper sogatella furcifera are both important pests on rice throughout asia. the major cause of recent outbreaks is thought to be the development of insecticide resistance. thus, the authors monitored insecticide susceptibilities in populations of these two insects immigrating into japan in the period 2005-2012. ten insecticides were tested, including members of the organophosphate, carbamate, pyrethroid, neonicotinoid and phenylp ... | 2014 | 23754555 |
| [geographical populations of brown planthopper in nanning of south china and in vietnam: a comparative study]. | a comparison was made on the differences of the geographical populations of brown planthopper (bph), nilaparvata lugens (stål), collected from nanning of south china and from ha noi, ha tinh, hue, tp. ho chi minh, and song cuu long of vietnam. the bph populations from nanning, ha noi, ha tinh and hue could damage the rice varieties with bph1 and bph2 genes, whereas the bph populations from tp. ho chi minh and song cuu long showed stronger virulence to the rice varieties with bph1, bph2, bph3 and ... | 2013 | 23718009 |
| [sublethal effect of chlorantraniliprole on the experimental population of non-target insect nilaparvata lugens (stål)]. | chlorantraniliprole is a newly developed insecticide targeting at lepidopteron pests in rice fields, whereas nilaparvata lugens (stål) is one of the important non-target pests of the insecticide. in this paper, the rice stem dipping method was adopted to test the toxicity of chlorantraniliprole to the 3rd instar nymphs and adults of n. lugens. the lc50 of chlorantraniliprole to the 3rd instar nymphs and adults was 26.85 and 35.53 mg.l-1, respectively. when the 3rd instar nymph was exposed to the ... | 2013 | 23705404 |
| conserved micrornas mir-8-5p and mir-2a-3p modulate chitin biosynthesis in response to 20-hydroxyecdysone signaling in the brown planthopper, nilaparvata lugens. | molting is an important developmental process in insects, usually along with synthesis and degradation of chitin. 20-hydroxyecdysone (20e), an insect hormone, has been reported to contribute to many processes including molting. however, little is known about the link between the chitin biosynthesis pathway and 20e signaling. here, we report that conserved mir-8-5p (mir-8-5p) and mir-2a-3p and their new target genes are critical for ecdysone-induced chitin biosynthesis in a hemipteran insect nila ... | 2013 | 23796434 |
| the genome sequence and transmission of an iflavirus from the brown planthopper, nilaparvata lugens. | a previously unknown iflavirus has been identified in a laboratory colony of the brown planthopper, nilaparvata lugens. the iflavirus-like sequence was first identified in contig sequences obtained from transcriptome sequencing (rna-seq) of the brown planthopper. the complete viral genome was resequenced using the sanger method. the positive-strand rna genome was 10,937 nucleotides excluding the 3' poly(a) tail, and contained a single large open reading frame encoding coat proteins in the 5' reg ... | 2013 | 23777870 |
| zero effect of bt rice on expression of genes coding for digestion, detoxification and immune responses and developmental performances of brown planthopper nilaparvata lugens (stål). | transgenic cry1ac, cry2aa and cry1ca (bt toxins) rice lines are well developed to manage lepidopteron pests in china. the impact of transgenic bt rice on the non-target brown planthopper (bph) has become an essential part of environmental risk assessment, however, scanty evidence is found addressing on developmental and molecular responses of bph to the ingestion of bt protein from transgenic rice. the focus of the current study is to examine the developmental characteristics and the expression ... | 2013 | 23920284 |
| influences of chlorpyrifos on antioxidant enzyme activities of nilaparvata lugens. | the brown planthoppers (nilaparvata lugens, bph) resistant to chlorpyrifos were selected in laboratory for eight generations. in the full course, the successive changes of activities of sod, cat and pod were analyzed. the analyses revealed that increasing of ld50 value was parallel to increasing of sod, cat, and pod, all of which increased gradually generation by generation. qrt-pcr showed that cat was not directly involved in chlorpyrifos detoxification, but could be transcriptionally induced b ... | 2013 | 24064262 |
| effects of two bt rice lines t2a-1 and t1c-19 on the ecological fitness and detoxification enzymes of nilaparvata lugens (hemiptera: delphacidae) from different populations. | the brown planthopper, nilaparvata lugens stål (hemiptera: delphacidae), exhibits geographic and temporal variations in its virulence and damage characteristics on cultivated rice varieties, so the potential ecological risk of the nontarget effectiveness of bt rice on different brown planthopper populations should be addressed. the nontarget effects of two bt rice lines t2a-1 (cry2a rice) and t1c-19 (cry1c rice) on six brown planthopper populations with geographical, virulent, and resistant vari ... | 2013 | 24020307 |
| genome-wide analysis of nilaparvata lugens nymphal responses to high-density and low-quality rice hosts. | the brown planthopper (bph) nilaparvata lugens is an economically important pest on rice plants. in this study, the higher population density and yellow-ripe stage of rice plants were used to construct adverse survival conditions (asc) against bph nymphs. simultaneously, the low population density and tillering stage of rice plants were used to establish a suitable survival condition (ssc) as a control. solexa/illumina sequencing was used to identify genes of bph nymphs responding to asc. signif ... | 2013 | 23956011 |
| molecular characterization and gene functional analysis of dicer-2 gene from nilaparvata lugens (hemiptera: geometroidea). | nilaparvata lugens (stål) (hemiptera: geometroidea), a serious rice pest in many countries of asia, causes a great loss in rice production every year. rna interference (rnai) is a powerful technology for gene function study in insects and a potential tool for pest control. as a core component of rnai pathway, dicer-2 (dcr-2) protein determines the production of small interfering rna (sirna) and is crucial for the efficiency of rnai. in this study, the full-length complementary dna (cdna) of n. l ... | 2013 | 23955826 |
| mitochondrial cox sequences of nilaparvata lugens and sogatella furcifera (hemiptera, delphacidae): low specificity among asian planthopper populations. | the brown planthoppers (bph) nilaparvata lugens (stål) and the white-backed planthoppers (wbph) sogatella furcifera (horváth) annually migrate from tropical and subtropical regions to temperate regions in asia, including japan, korea and northern china. to elucidate the genetic divergence based on geography of planthoppers and to estimate their migration route on the basis of molecular data, we analysed a part of their mitochondrial genome sequences. sequences of cytochrome oxidase subunit i (co ... | 2013 | 23537548 |
| the influence of typhoon khanun on the return migration of nilaparvata lugens (stål) in eastern china. | migratory insects adapt to and exploit the atmospheric environment to complete their migration and maintain their population. however, little is known about the mechanism of insect migration under the influence of extreme weather conditions such as typhoons. a case study was conducted to investigate the effect of typhoon khanun, which made landfall in the eastern china in sept. 2005, on the migration of brown planthopper, nilaparvata lugens (stål). the migration pathways of n. lugens were recons ... | 2013 | 23468954 |
| sublethal effects of paichongding on nilaparvata lugens (homoptera: delphacidae). | nilaparvata lugens (stål) is a major rice pest in asia paichongding is a novel neonicotinoid insecticide developed in 2008. the effects of this insecticide on life-table parameters and wing formation of n. lugens were examined in the laboratory. the results showed that paichongding could significantly reduce the fecundity of n. lugens, demonstrating further activity against this pest in addition to direct toxicity. the fecundity of n. lugens treated with lc20, lc30, lc40, and lc50 of paichongdin ... | 2013 | 23448009 |
| a simple sequence repeat- and single-nucleotide polymorphism-based genetic linkage map of the brown planthopper, nilaparvata lugens. | in this study, we developed the first genetic linkage map for the major rice insect pest, the brown planthopper (bph, nilaparvata lugens). the linkage map was constructed by integrating linkage data from two backcross populations derived from three inbred bph strains. the consensus map consists of 474 simple sequence repeats, 43 single-nucleotide polymorphisms, and 1 sequence-tagged site, for a total of 518 markers at 472 unique positions in 17 linkage groups. the linkage groups cover 1093.9 cm, ... | 2013 | 23204257 |
| molecular basis for insecticide-enhanced thermotolerance in the brown planthopper nilaparvata lugens stål (hemiptera:delphacidae). | climate change is likely to have marked ecological effects on terrestrial ecosystems, including the activities of insect pests. most attention has focused on the increasing geographical ranges of pests; however, if extrinsic factors enhance their thermotolerance, populations may express increased voltinism and longer daily and annual activity periods. these changes in pest populations have the potential for severe consequences, including increased crop losses and decreased food security at the g ... | 2013 | 24303791 |
| single-feature polymorphism mapping of isogenic rice lines identifies the influence of terpene synthase on brown planthopper feeding preferences. | bph3, a major brown planthopper (bph) resistance locus derived from the rice cultivar rathu heenati (rh), has been used as a stable donor of traits that improve highly susceptible aromatic rice varieties in thailand. map-based cloning was initiated using a set of isogenic lines (ils) harboring the major bph3 locus on chromosome 6. il genomes were scanned with a 57 k affymetrix rice genechip to identify the gene responsible for bph3. | 2013 | 24280452 |
| comparative transcriptome analysis of salivary glands of two populations of rice brown planthopper, nilaparvata lugens, that differ in virulence. | the brown planthopper (bph), nilaparvata lugens (stål), a destructive rice pest in asia, can quickly overcome rice resistance by evolving new virulent populations. herbivore saliva plays an important role in plant-herbivore interactions, including in plant defense and herbivore virulence. however, thus far little is known about bph saliva at the molecular level, especially its role in virulence and bph-rice interaction. | 2013 | 24244529 |
| dicer1 is crucial for the oocyte maturation of telotrophic ovary in nilaparvata lugens (stål) (hemiptera: geometroidea). | as a member of the rnase iii nucleases family, dicer1 (dcr1) protein plays an essential role in the production of micrornas (mirnas) and oocyte development. here, the full-length cdna of nilaparvata lugens dcr1 (nldcr1) was firstly cloned and analyzed, and then the function of nldcr1 gene was investigated by rnai. the open reading frame of nldcr1 cdna was 6,720 bp in length (genbank accession no. jx644040), which encoded for a protein of 2,239 amino acids. the nldcr1 transcripts were present in ... | 2013 | 24132808 |
| proteomic and transcriptomic analyses of fecundity in the brown planthopper nilaparvata lugens (stål). | as an r-strategy insect species, the brown planthopper (bph) nilaparvata lugens (stål) is a serious pest of rice crops in the temperate and tropical regions of asia and australia, which may be due to its robust fecundity. here we combined 2-de comparative proteomic and rna-seq transcriptomic analyses to identify fecundity-related proteins and genes. using high- and low-fecundity populations as sample groups, a total of 54 and 75 proteins were significantly altered in the third and sixth day brac ... | 2013 | 24083549 |
| feeding-induced interactions between two rice planthoppers, nilaparvata lugens and sogatella furcifera (hemiptera: delphacidae): effects on feeding and honeydew excretion. | a series of laboratory experiments was conducted using electrical penetration graph, salivary flange, and honeydew measurement to study the effects of feeding-induced intra- and interspecific interactions on feeding behavior and honeydew excretion between planthoppers nilaparvata lugens (stål) and sogatela furcifera (horváth). feeding-induced intra- and interspecific interactions affected many measures of feeding behavior. the number of salivary flanges, mean duration of pathway activities per i ... | 2013 | 24468558 |
| feeding-induced interactions between nilaparvata lugens and laodelphax striatellus (hemiptera: delphacidae): effects on feeding behavior and honeydew excretion. | using electrical penetration graph, salivary flange, and honeydew measurement, this study investigated the effects of feeding-induced intra- and interspecific interactions on feeding behavior and honeydew excretion of brown planthopper (nilaparvata lugens) compared with small brown planthopper (laodelphax striatellus). results showed that many measures of feeding behavior were affected by feeding-induced intra- and interspecific interactions on two different rice varieties. there were significan ... | 2013 | 24331608 |
| male killing caused by a spiroplasma symbiont in the small brown planthopper, laodelphax striatellus. | spiroplasma-mediated late male killing was found in the small brown planthopper, laodelphax striatellus. female-biased colonies (maternal lines, n = 4) were established from planthoppers collected in taiwan and japan. this sex ratio distortion was maternally inherited (sex ratio of total number of progenies [female:male]: 488:0 in f1, 198:7 in f2, 407:0 in f3; likelihood ratio test of all generations, p < 0.0001) and caused by male death during nymphal stages. the female-biased colonies were dou ... | 2013 | 23975837 |
| high temperature determines the ups and downs of small brown planthopper laodelphax striatellus population. | small brown planthopper, laodelphax striatellus (fallén) numbers usually drop sharply in the summer and revive quickly in the autumn. however, it is unclear whether and how the high temperature plays a role in this process. the effects of durations of heat exposure (33°c) on life-history traits were examined here. exposure of adults for 1 day during the oviposition stage led to a very low survival of nymphs. the average longevity of l. striatellus exposed for 1-31 days from oviposition was signi ... | 2013 | 23955890 |
| various infection status and molecular evidence for horizontal transmission and recombination of wolbachia and cardinium among rice planthoppers and related species. | wolbachia and cardinium are widely distributed and are considered important for their ability to disturb reproduction and affect other fitness-related traits of their hosts. by using multilocus sequence typing (mlst), rflp (restriction fragment length polymorphism) and 16s ribosomal dna gene sequencing methods, we extensively surveyed wolbachia and cardinium infection status of four predominant rice planthoppers and one kind of leafhopper in different rice fields. the results demonstrated that s ... | 2013 | 23955885 |
| the complete mitochondrial genomes of two rice planthoppers, nilaparvata lugens and laodelphax striatellus: conserved genome rearrangement in delphacidae and discovery of new characteristics of atp8 and trna genes. | nilaparvata lugens (the brown planthopper, bph) and laodelphax striatellus (the small brown planthopper, sbph) are two of the most important pests of rice. up to now, there was only one mitochondrial genome of rice planthopper has been sequenced and very few dependable information of mitochondria could be used for research on population genetics, phylogeographics and phylogenetic evolution of these pests. to get more valuable information from the mitochondria, we sequenced the complete mitochond ... | 2013 | 23799924 |
| development and preliminary application of a triplex real-time polymerase chain reaction assay for evaluating predation on three planthoppers in a rice ecosystem. | a multiplex real-time quantitative polymerase chain reaction (pcr) assay was developed to simultaneously detect the dna of three rice planthoppers, that is, sogatella furcifera (horváth) (white-backed planthopper), nilaparvata lugens (stål) (brown planthopper) and laodelphax striatellus (fallén) (small brown planthopper), in the gut of their predators. the sets of primers and allglo probes were targeted to the regions of internal transcribed spacer 2 (its2) genes in nuclear ribosomal dna (rdna). ... | 2013 | 23711378 |
| identification and biochemical characterization of laodelphax striatellus neutral ceramidase. | ceramidases are a group of enzymes that catalyse hydrolysis of ceramides to generate fatty acid and sphingosine. in this study, we report the cloning and characterization of the rice small brown planthopper laodelphax striatellus neutral ceramidase (ncdase), lsncer. lsncer was identified by sequencing the transcriptome of l. striatellus and is a protein of 717 amino acids with a predicted molecular weight of 79.3 kda. similarly to other known ncdases, the optimum ph for lsncer is 8.0 and the opt ... | 2013 | 23601004 |
| identification of a new locus conferring antixenosis to the brown planthopper in rice cultivar swarnalata (oryza sativa l.). | the brown planthopper [nilaparvata lugens (stål); bph] has caused severe damage to rice production. the identification of resistance genes and the development of bph-resistant varieties are economical and effective ways to manage this pest. using an f2 population from a cross between the indica cultivars 93-11 and swarnalata, we mapped the qbph-8 locus to a 7.3-cm region on chromosome 8 in two tests, flanked by the markers rm339 and rm515. in this population, qbph-8 explained 7.7 and 6.6% of the ... | 2013 | 24065663 |
| evidence of sibling species between two host-associated populations of brown planthopper, n. lugens (stål) (homoptera: delphacidea) complex based on morphology and host-plant relationship studies. | morphological and host-plant relationship studies were conducted to differentiate two sympatric populations of brown planthopper (bph), nilaparvata lugens, one from rice (oryza sativa) and the other from leersia hexandra, a weed grass. in morphometric studies based on esterase activities, an upgma dendrogram using 17 quantitative morphological characters, including stridulatory organs (courtship signal-producing organs) between two sympatric populations of n. lugens, one from rice and the other ... | 2013 | 23932255 |
| an injury equivalency system for establishing a common economic threshold for three species of rice planthoppers (hemiptera: delphacidae) in taiwan. | the economic threshold (et) for multiple pest species that share the same injury type on host plants (feeding guild) has been proposed for decision-making in integrated management framework of many defoliating insect pests. however, only a few consider agricultural pests with sucking mouthparts. this study presents the first injury equivalency system for the feeding guild made up of three rice (oryza sativa l.) planthopper (hemiptera: delphacidae) species--nilaparvata lugens (stål), sogatella fu ... | 2013 | 23786072 |
| glutathione s-transferase of brown planthoppers (nilaparvata lugens) is essential for their adaptation to gramine-containing host plants. | plants have evolved complex processes to ward off attacks by insects. in parallel, insects have evolved mechanisms to thwart these plant defenses. to gain insight into mechanisms that mediate this arms race between plants and herbivorous insects, we investigated the interactions between gramine, a toxin synthesized by plants of the family gramineae, and glutathione s transferase (gst), an enzyme found in insects that is known to detoxify xenobiotics. here, we demonstrate that rice (oryza sativa) ... | 2013 | 23700450 |
| proteome analysis of rice (oryza sativa l.) mutants reveals differentially induced proteins during brown planthopper (nilaparvata lugens) infestation. | although rice resistance plays an important role in controlling the brown planthopper (bph), nilaparvata lugens, not all varieties have the same level of protection against bph infestation. understanding the molecular interactions in rice defense response is an important tool to help to reveal unexplained processes that underlie rice resistance to bph. a proteomics approach was used to explore how wild type ir64 and near-isogenic rice mutants with gain and loss of resistance to bph respond durin ... | 2013 | 23434671 |
| towards understanding of molecular interactions between rice and the brown planthopper. | the brown planthopper (bph) is the most notorious pest of rice (oryza sativa). studies of rice-bph interaction have contributed to development of new rice varieties, offering an effective means for long-lasting control of bph. here, we review the status of knowledge of the molecular basis of rice-bph interaction, from the perspective of immunity. the bph has complicated feeding behaviors on rice, which are mainly related to host resistance. now, 24 resistance genes have been detected in rice, in ... | 2013 | 23396040 |
| development and validation of a pcr-based functional marker system for the brown planthopper resistance gene bph14 in rice. | brown planthopper (bph) is the most damaging rice pest globally. resistant varieties are the most effective and environmental strategy for protecting the rice crop from bph. functional markers (fms) designed from polymorphic sites within gene sequences affecting phenotypic variation are highly efficient when used for marker assisted selection (mas). bph14 is the first and only cloned insect resistance gene so far in rice. compared to the sequences of its non-effective alleles there are a number ... | 2013 | 24273431 |
| pyramiding and evaluation of three dominant brown planthopper resistance genes in the elite indica rice 9311 and its hybrids. | brown planthopper (bph), nilaparvata lugens stål, is the most devastating insect pest in rice-producing areas. three dominant bph resistance genes (bph14, bph15, bph18) were pyramided into elite indica rice 9311 and its hybrids using marker-assisted selection. gene effectiveness was evaluated on the basis of seedling and adult rice resistance, honeydew weight and survival rate of bph. | 2013 | 23175467 |
| fine mapping and characterization of bph27, a brown planthopper resistance gene from wild rice (oryza rufipogon griff.). | the brown planthopper (nilaparvata lugens stål; bph) is one of the most serious rice pests worldwide. growing resistant varieties is the most effective way to manage this insect, and wild rice species are a valuable source of resistance genes for developing resistant cultivars. bph27 derived from an accession of guangxi wild rice, oryza rufipogon griff. (accession no. 2183, hereafter named gx2183), was primarily mapped to a 17-cm region on the long arm of the chromosome four. in this study, fine ... | 2013 | 23001338 |
| osmpk3 positively regulates the ja signaling pathway and plant resistance to a chewing herbivore in rice. | key message : silencing osmpk3 decreased elicited ja levels, which subsequently reduced levels of herbivore-induced trypsin protease inhibitors (tryppis) and improved the performance of ssb larvae, but did not influence bph. mitogen-activated protein kinases (mpks) are known to play an important role in plant defense by transferring biotic and abiotic signals into programmed cellular responses. however, their functions in the herbivore-induced defense response in rice remain largely unknown. her ... | 2013 | 23344857 |
| molecular characterization of the flightin gene in the wing-dimorphic planthopper, nilaparvata lugens, and its evolution in pancrustacea. | flightin was initially identified in drosophila melanogaster. previous work has shown that drosophila flightin plays a key role in indirect flight muscle (ifm) function and has limited expression in the ifm. in this study, we demonstrated that flightin is conserved across the pancrustacea species, including winged insects, non-winged insects, non-insect hexapods and several crustaceans. the brown planthopper (bph), nilaparvata lugens (stål) (hemiptera: delphacidae), a long-distance migration ins ... | 2013 | 23459170 |
| molecular cloning and mrna expression of a ryanodine receptor gene in the cotton bollworm, helicoverpa armigera. | ryanodine receptors (ryrs) are the targets of novel diamide insecticides. the cotton bollworm, helicoverpa armigera, is one of the most important cotton pests in the world. in this study, we report the full-length ryr cdna sequence (named as haryr) of h. armigera. the 16,083-bp contiguous sequence encoded 5, 142 amino acid residues, which shares 80% and 78% overall identities with its homologues in nilaparvata lugens (nlryr) and drosophila melanogaster (dmryr), respectively. all hallmarks of ryr ... | 2013 | 24267694 |
| genome-wide screening for components of small interfering rna (sirna) and micro-rna (mirna) pathways in the brown planthopper, nilaparvata lugens (hemiptera: delphacidae). | the brown planthopper (bph), nilaparvata lugens, is a major rice pest in asia, and accumulated evidence indicates that this species is susceptible to rna interference (rnai); however, the mechanism underlying rnai and parental rnai has not yet been determined. we comprehensively investigated the repertoire of core genes involved in small interfering rna (sirna) and micro-rna (mirna) pathways in the bph by comparing its newly assembled transcriptome and genome with those of drosophila melanogaste ... | 2013 | 23937246 |
| cloning and characterization of two genes coding for the histone acetyltransferases, elp3 and mof, in brown planthopper (bph), nilaparvata lugens (stål). | histone acetylation is a vital mechanism for the post-translational modifications of chromatin components. histone acetyltransferases (hats) are critical elements that determine histone acetylation and regulate chromatin dynamics and gene expression. while histone acetyltransferases have been well studied in mammals and drosophila melanogaster, information from agriculturally important insect pests is still limited. in our effort to understand the epigenetic mechanisms regulating development in ... | 2013 | 23142031 |
| nicotinic acetylcholine receptor beta1 subunit from the brown planthopper, nilaparvata lugens: a-to-i rna editing and its possible roles in neonicotinoid sensitivity. | nicotinic acetylcholine (ach) receptors (nachrs) are ligand-gated ion channels which mediate fast cholinergic synaptic transmission in insect and vertebrate nervous systems. the nachr agonist-binding site is formed by loops a-c present in alpha subunits together with loops d-f present in either non-alpha subunits or homomer-forming alpha subunits. a new non-alpha subunit was cloned from nilaparvata lugens, a major rice pest in many parts of asia, showing very high amino acid identity to other in ... | 2013 | 19254762 |
| characterization of rice black-streaked dwarf virus- and rice stripe virus-derived sirnas in singly and doubly infected insect vector laodelphax striatellus. | replication of rna viruses in insect cells triggers an antiviral defense that is mediated by rna interference (rnai) which generates viral-derived small interfering rnas (sirnas). however, it is not known whether an antiviral rnai response is also induced in insects by reoviruses, whose double-stranded rna genome replication is thought to occur within core particles. deep sequencing of small rnas showed that when the small brown planthopper (laodelphax striatellus) was infected by rice black-str ... | 2013 | 23776591 |
| analysis of yeast-like symbiote diversity in the brown planthopper (bph), nilaparvata lugens stål, using a novel nested pcr-dgge protocol. | yeast-like symbiotes (yls) are endosymbionts that are intimately associated with the growth, development, reproduction of their host, the brown planthopper, nilaparvata lugens (hemiptera: delphacidae). however, it is unclear how many species of yls are found within n. lugens, and how they are related to each other. traditional methods or simple amplification based on 18s rdna sequence does not reliably identify new species quickly and efficiently. therefore, a novel nested pcr-denaturing gradien ... | 2013 | 23549902 |
| biochemical mechanisms of imidacloprid resistance in nilaparvata lugens: over-expression of cytochrome p450 cyp6ay1. | imidacloprid is a key insecticide extensively used for control of nilaparvata lugens, and its resistance had been reported both in the laboratory selected strains and field populations. a target site mutation y151s in two nicotinic acetylcholine receptor subunits and enhanced oxidative detoxification have been identified in the laboratory resistant strain, contributing importantly to imidacloprid resistance in n. lugens. to date, however, imidacloprid resistance in field population is primarily ... | 2013 | 23994173 |
| migration of rice planthoppers and their vectored re-emerging and novel rice viruses in east asia. | this review examines recent studies of the migration of three rice planthoppers, laodelphax striatellus, sogatella furcifera, and nilaparvata lugens, in east asia. laodelphax striatellus has recently broken out in jiangsu province, eastern china. the population density in the province started to increase in the early 2000s and peaked in 2004. in 2005, rice stripe virus (rsv) viruliferous rate of l. striatellus peaked at 31.3%. since then, rice stripe disease spread severely across the whole prov ... | 2013 | 24312081 |
| overexpression of rice black-streaked dwarf virus p7-1 in arabidopsis results in male sterility due to non-dehiscent anthers. | rice black-streaked dwarf virus (rbsdv), a member of the genus fijivirus in the family reoviridae, is propagatively transmitted by the small brown planthopper (laodelphax striatellus fallén). rbsdv causes rice black-streaked dwarf and maize rough dwarf diseases, which lead to severe yield losses in crops in china. although several rbsdv proteins have been studied in detail, the functions of the nonstructural protein p7-1 are still largely unknown. to investigate the role of the p7-1 protein in v ... | 2013 | 24260239 |
| southern rice black-streaked dwarf virus: a white-backed planthopper-transmitted fijivirus threatening rice production in asia. | southern rice black-streaked dwarf virus (srbsdv), a non-enveloped icosahedral virus with a genome of 10 double-stranded rna segments, is a novel species in the genus fijivirus (family reoviridae) first recognized in 2008. rice plants infected with this virus exhibit symptoms similar to those caused by rice black-streaked dwarf virus. since 2009, the virus has rapidly spread and caused serious rice losses in east and southeast asia. significant progress has been made in recent years in understan ... | 2013 | 24058362 |
| synthesis and biological evaluation of nitromethylene neonicotinoids based on the enhanced conjugation. | the neonicotinoids with a nitroconjugated system had excellent bioactivity, which could rival imidacloprid, and has been previously reported. however, the photodegradation and hydrolysis of this series of neonicotinoids was very quick according to our further investigation, which cannot be developed as a pesticide further. the approach to further enhance the conjugation was tried not only to increase the bioactivities but also to improve the stability in water and in the sun. a substituted pheny ... | 2013 | 24180522 |
| knockdown of a putative halloween gene shade reveals its role in ecdysteroidogenesis in the small brown planthopper laodelphax striatellus. | ecdysteroid hormone 20-hydroxyecdysone (20e) plays fundamental roles in insect development and reproduction, whereas the primary role of ecdysone (e) is the precursor for 20e. a cytochrome p450 monooxygenase (cyp), encoded by a halloween gene shade (shd, cyp314a1), catalyzes the conversion of e into 20e in representative insect species in diptera, lepidoptera and orthoptera. we describe here the cloning and characterization of lsshd in a hemipteran insect species, the small brown planthopper lao ... | 2013 | 24055487 |
| comparative transcriptome analysis of two rice varieties in response to rice stripe virus and small brown planthoppers during early interaction. | rice stripe, a virus disease, transmitted by a small brown planthopper (sbph), has greatly reduced production of japonica rice in east asia, especially in china. although we have made great progress in mapping resistance genes, little is known about the mechanism of resistance. by de novo transcriptome assembling, we gained sufficient transcript data to analyze changes in gene expression of early interaction in response to sbph and rsv infection in rice. respectively 648 and 937 degs were detect ... | 2013 | 24358146 |
| investigation on subcellular localization of rice stripe virus in its vector small brown planthopper by electron microscopy. | rice stripe virus (rsv), which is transmitted by small brown planthopper (laodelphax striatellus fallén, sbph), has been reported to be epidemic and cause severe rice stripe disease in rice fields in many east asian countries, including china. investigation on viral localization in the vector is very important for elucidating transmission mechanisms of rsv by sbph. in this study, transmission electron microscopy and immuno-gold labeling technique were used to investigate the subcellular localiza ... | 2013 | 24139455 |
| detection of quantitative trait loci (qtls) for resistances to small brown planthopper and rice stripe virus in rice using recombinant inbred lines. | small brown planthopper (sbph) and rice stripe virus (rsv) disease transmitted by sbph cause serious damage to rice (oryza sativa l.) in china. in the present study, we screened 312 rice accessions for resistance to sbph. the indica variety, n22, is highly resistant to sbph. one hundred and eighty two recombinant inbred lines (rils) derived from a cross of n22 and the highly susceptible variety, ussr5, were used for quantitative trait locus (qtl) analysis of resistances to sbph and rsv. in a mod ... | 2013 | 23591851 |