Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| effects of preovulatory follicle size on estradiol concentrations, corpus luteum diameter, progesterone concentrations and subsequent pregnancy rate in buffalo cows (bubalus bubalis). | the present study was undertaken to investigate the effects of preovulatory follicle (pof) size on estradiol concentrations, luteal profile (cl diameter and progesterone concentration) and subsequent pregnancy rate in murrah buffalo cows. the buffalo cows (n = 49) were synchronized for estrus by two doses of pgf2α given 11 days apart. the buffalo cows were inseminated during standing estrus and again after 24 h. ovaries were scanned at estrus and 24 h intervals until ovulation, thereafter on day ... | 2018 | 29128702 |
| in-gel and offgel-based proteomic approach for authentication of meat species from minced meat and meat products. | fraudulent mislabelling of processed meat products on a global scale that cannot be detected using conventional techniques necessitates sensitive, robust and accurate methods of meat authentication to ensure food safety and public health. in the present study, we developed an in-gel (two-dimensional gel electrophoresis, 2de) and offgel-based proteomic method for authenticating raw and cooked water buffalo (bubalus bubalis), sheep (ovis aries) and goat (caprus hircus) meat and their mixes. | 2018 | 28737240 |
| retinoic acid induces differentiation of buffalo (bubalus bubalis) embryonic stem cells into germ cells. | development of precise and reproducible culture system for in vitro differentiation of embryonic stem (es) cells into germ cells counts as a major leap forward for understanding not only the remarkable process of gametogenesis, otherwise obscured by limited availability of precursor primordial germ cells (pgcs), but in finally treating the catastrophic infertility. taking into account the significant role of retinoic acid (ra) during in vivo gametogenesis, we designed the present study to invest ... | 2017 | 28736154 |
| genomic differentiation between swamp and river buffalo using a cattle high-density single nucleotide polymorphisms panel. | buffalo (bubalus bubalis) is an important livestock species in many tropical and subtropical regions. in recent decades, the interest in buffalo's milk have expanded and intensive buffalo farms start to emerge. however, breeding programs and population genetics information for this species is scarce or inexistent. the present study aims to test the suitability of the commercial high-density single nucleotide polymorphisms (snp) genotyping panel, the illumina bovinehd beadchip, to estimate popula ... | 2017 | 28735584 |
| influence of body condition score at calving on the metabolic status and production performance of murrah buffaloes (bubalus bubalis) during the transition period. | the purpose of this study was to evaluate the influence of body condition score (bcs) at calving on the metabolic status of female murrah buffaloes in the transition period. | 2017 | 28728402 |
| ultrastructure changes in buffalo (bubalus bubalis) oocytes before and after maturation in vitro with sericin. | the aim of this research was to identify the changes in the cytoplasmic ultrastructure of immature and matured oocytes in buffalo (bubalus bubalis). oocytes were matured in vitro in tissue culture medium-199 with and without sericin, and then analyzed by light and transmission electron microscopy. the experiment result showed that the nuclear maturation rate of buffalo oocytes was significantly higher in the presence of sericin (80.6%) than without sericin (68.1%) (p < 0.05). the immature oocyte ... | 2017 | 28722264 |
| expression and molecular cloning of interferon stimulated genes in buffalo (bubalus bubalis). | buffalo, the most important livestock species in tropical india, remains to be a poor breeder mainly due to embryonic mortality (65%) occurring mostly between 16 and 18 days of pregnancy. early and accurate diagnosis of pregnancy can thus become a boon for successful herd management in buffalo. however, most of the currently available methods allow diagnosis only after 30 days post ai. interferon tau (ifnt), the first pregnancy recognition signal in ruminants is one such molecule, which stimulat ... | 2017 | 28708533 |
| whole mitogenomes reveal the history of swamp buffalo: initially shaped by glacial periods and eventually modelled by domestication. | the newly sequenced mitochondrial genomes of 107 asian swamp buffalo (bubalus bubalis carabensis) allowed the reconstruction of the matrilineal divergence since ~900 kya. phylogenetic trees and bayesian skyline plots suggest a role of the glacial periods in the demographic history of swamp buffalo. the ancestral swamp-buffalo mitogenome is dated ~232 ± 35 kya. two major macro-lineages diverged during the 2nd pleistocene glacial period (~200-130 kya), but most (~99%) of the current matrilines der ... | 2017 | 28680070 |
| comparative efficacy of oestrus synchronization protocols in buffalo (bubalus bubalis). | this study was conducted to test the efficacy of gonadotropic hormone (gnrh)-based synchronization protocols (ovsynch, heatsynch, and ovsynch plus) in buffaloes under field condition. based on anamnesis and transrectal palpation twice at 10-day interval and serum progesterone (p4) concentration, 150 anoestrous buffaloes and delayed pubertal heifers were selected to induce oestrus using gnrh-based protocols. these selected animals were randomly divided into three groups: group i: ovsynch (n = 50) ... | 2017 | 28669062 |
| ovum pick-up interval in buffalo (bubalus bubalis) managed under wetland conditions in argentina: effect on follicular population, oocyte recovery, and in vitro embryo development. | the excellent adaptation of water buffalo (bubalis bubalis) to swampy environments means that animals are frequently managed in areas with restricted access for reproductive procedures. the objective of this study was to evaluate the effect of the ovum pick-up (opu) interval on follicular population, oocyte recovery, oocyte quality and in vitro embryo production. twelve murrah buffaloes were subjected to two consecutive dominant follicle reductions, and randomly assigned to either 7-day (n=6) or ... | 2017 | 28662882 |
| cryopreservation of nili-ravi buffalo (bubalus bubalis) semen in andromed® extender; in vitro and in vivo evaluation. | the study was designed to evaluate andromed® for the freezability and fertility of nili-ravi buffalo semen. semen was collected from four adult nili-ravi buffalo (bubalus bubalis) bulls for 3 weeks (replicate). semen ejaculates from each buffalo bull were divided into three aliquots. one aliquot was used for evaluation of motility, plasma membrane integrity, livability, viability, dna integrity and normal apical ridge. remaining two aliquots were diluted (37°c; 50 × 106 spermatozoa/ml) in tris-c ... | 2017 | 28660630 |
| expression, localization of sumo-1, and analyses of potential sumoylated proteins in bubalus bubalis spermatozoa. | mature spermatozoa have highly condensed dna that is essentially silent both transcriptionally and translationally. therefore, post translational modifications are very important for regulating sperm motility, morphology, and for male fertility in general. protein sumoylation was recently demonstrated in human and rodent spermatozoa, with potential consequences for sperm motility and dna integrity. we examined the expression and localization of small ubiquitin-related modifier-1 (sumo-1) in the ... | 2017 | 28659810 |
| pcr-sscp analysis of mdgi gene and its association with milk production traits in river buffalo (bubalus bubalis). | in this study, we investigated the genetic variation within 3'utr of mammary-derived growth inhibitor (mdgi) gene of buffalo using pcr-sscp and sequencing; and also analyzed association of polymorphism with the milk production traits. the study revealed two conformational patterns, 'a' and 'b' among 234 mehsana buffaloes maintained with their records in the field and at farm. the frequency of sscp variant 'a' was found to be invariably high in the buffalo population under study. further, associa ... | 2017 | 28647599 |
| prevalence and microscopic studies of sarcocystis infection in naturally infected water buffaloes (bubalus bubalis) of andhra pradesh. | sarcocystis species are the obligate tissue protozoan parasites of livestock causing clinical and subclinical disease resulting in downgrading of the meat and their products thereby leading to economic losses. the present study reveals the prevalence and distribution of sarcocystosis in water buffaloes (bubalus bubalis) slaughtered at local abattoirs in a.p for a period of 1 year from june 2014 to may 2015. a total of 137 buffalo carcasses were screened grossly and microscopically organ wise viz ... | 2017 | 28615863 |
| pregnancy-associated glycoproteins (pags) concentrations in water buffaloes (bubalus bubalis) during gestation and the postpartum period. | for the first time in literature this study describes the pregnancy-associated glycoprotein (pag) profile of buffalo cows during gestation and the post-partum period using antiserum raised against pag-molecules purified from buffalo placenta (as#860). ninety-eight buffalo cows, belonging to a buffalo herd subjected to a synchronization and artificial insemination (ai) program, were enrolled in this study. blood samples were taken on days 0 (ai), 23, 25, 28, 30 and then biweekly until the end of ... | 2017 | 28583611 |
| hematologic ris for healthy water buffaloes (bubalus bubalis) in southern mexico. | baseline hematologic data are used by veterinarians and wildlife biologists to perform health assessments on target animal species. hematologic measurements are influenced by various factors including geography. baseline hematologic ris have been established for domestic water buffaloes (bubalus bubalis) from various countries in the eastern hemisphere, but these data are not readily available for the western hemisphere. | 2017 | 28582601 |
| comprehensive characterization of bioactive peptides from buffalo (bubalus bubalis) colostrum and milk fat globule membrane proteins. | milk fat is dispersed in milk as small spherical globules stabilized in the form of emulsion by its surrounding membrane, often referred to as fat globule membrane (fgm). buffalo, a major milking mammal of asia and second most milking mammal across the globe presents physicochemical features different from that of other ruminant species containing higher content of lipids and proteins. the present study describes characterization of fgm proteins isolated from both buffalo milk and colostrum. a d ... | 2017 | 28578070 |
| comparison of two methods of seminal plasma removal on buffalo (bubalus bubalis) sperm cryopreservation. | cryopreservation causes damage to spermatozoa, and methods minimizing this damage are therefore needed. although much discussed, seminal plasma removal has become an alternative to improve sperm quality and viability after freezing and has been applied to different species in attempt to obtain good results. the objective of this study was to evaluate semen quality in buffaloes submitted to two methods for seminal plasma removal (filtration and centrifugation). semen samples were collected from s ... | 2017 | 28543808 |
| detection and localization of regucalcin in spermatozoa of water buffalo (bubalus bubalis): a calcium-regulating multifunctional protein. | regucalcin (rgn) is a calcium-regulating, anti-apoptotic, antioxidative and antiproliferative multifunctional protein predominantly seen in liver and kidney. all these functions are very crucial during spermatogenesis and sperm maturation process until fertilization of the ovum. although many studies have reported the wide distribution of regucalcin in the male reproductive tract of the rat, human and bovine, its presence in spermatozoa is yet to be demonstrated wherein calcium has a pivotal rol ... | 2017 | 28543743 |
| clinical efficacy and pharmacokinetics of meloxicam in mediterranean buffalo calves (bubalus bubalis). | the aims of the investigation were to establish for the first time (i) clinical efficacy and (ii) pharmacokinetic profile of meloxicam intravenously (iv) administered in male mediterranean buffalo calves after surgical orchiectomy. the study was performed on 10 healthy buffalo calves, between 4 and 5 months old and between 127 and 135 kg of body weight (b.w.). an iv injection of 0.5 mg/kg b.w. of meloxicam was administered in six calves (treated group, tg) immediately after surgery; the other fo ... | 2017 | 29077759 |
| repertoire of noncoding rnas in corpus luteum of early pregnancy in buffalo (bubalus bubalis). | the present study was designed to identify other noncoding rnas (ncrnas) in the corpus luteum (cl) during early pregnancy in buffalo. | 2017 | 29062204 |
| genome assembly and transcriptome resource for river buffalo, bubalus bubalis (2n = 50). | water buffalo is a globally important species for agriculture and local economies. a de novo assembled, well-annotated reference sequence for the water buffalo is an important prerequisite for studying the biology of this species, and is necessary to manage genetic diversity and to use modern breeding and genomic selection techniques. however, no such genome assembly has been previously reported. there are 2 species of domestic water buffalo, the river (2 n = 50) and the swamp (2 n = 48) buffalo ... | 2017 | 29048578 |
| buffalo heifers selected for lower residual feed intake have lower feed intake, better dietary nitrogen utilisation and reduced enteric methane production. | this study was conducted to evaluate the utilisation of the residual feed intake (rfi) as a feed efficiency selection tool and its relationship with methane emissions. eighteen murrah buffalo (bubalus bubalis) heifers were fed ad libitum with total mixed ration (tmr) for 120 days. based on linear regression models involving dry matter intake (dmi), average daily gain (adg) and mid-test metabolic body size (mbw0.75 ), heifers were assigned into low and high rfi groups. the rfi varied from -0.09 t ... | 2017 | 29027698 |
| design and validation of a 90k snp genotyping assay for the water buffalo (bubalus bubalis). | the availability of the bovine genome sequence and snp panels has improved various genomic analyses, from exploring genetic diversity to aiding genetic selection. however, few of the snp on the bovine chips are polymorphic in buffalo, therefore a panel of single nucleotide dna markers exclusive for buffalo was necessary for molecular genetic analyses and to develop genomic selection approaches for water buffalo. the creation of a 90k snp panel for river buffalo and testing in a genome wide assoc ... | 2017 | 28981529 |
| the functional and molecular studies on involvement of hydrogen sulphide in myometrial activity of non-pregnant buffaloes (bubalus bubalis). | hydrogen sulphide (h2s), a member of the gasotransmitters family, is known to play patho-physiological role in different body systems including during pregnancy. but its involvement in myometrial spontaneity and associated signalling pathways in uterus in non-pregnant animals is yet to be studied. present study describes the effect of l-cysteine, an endogenous h2s donor, on isolated myometrial strips of non-pregnant buffaloes and the underlying signaling mechanism(s). | 2017 | 29207994 |
| an association analysis between prl genotype and milk production traits in italian mediterranean river buffalo. | this research communication describes the association between genetic variation within the prolactin (prl) gene and the milk production traits of italian mediterranean river buffalo (bufala mediterranea italiana). high resolution melting (hrm) techniques were developed for genotyping 465 buffaloes. the association of genetic polymorphism with milk production traits was performed and subsequently the effects of parity and calving season were evaluated. single nucleotide polymorphisms (snps) were ... | 2017 | 29154740 |
| pregnancy rate in water buffalo following fixed-time artificial insemination using new or used intravaginal devices with two progesterone concentrations. | this study evaluated the pregnancy rate (pr) after timed artificial insemination (tai) in water buffalo (bubalus bubalis) during both non-breeding and breeding season, using either a new or reused intravaginal device (ivd) with two different progesterone concentrations. a total of 247 dairy buffalo cows were randomly assigned using a two-by-three factorial design and four replicates to the following groups: (1) new intravaginal device (ivd-new: dib®, 1.0 g of p4, n = 51 or cidr®, 1.38 g of p4, n ... | 2017 | 29274053 |
| virus neutralization technique as a tool to evaluate the virological profile for bovine viral diarrhea virus infection in dairy water buffalo (bubalus bubalis) herds. | serological evidence shows that the bovine viral diarrhea virus (bvdv) infections are present in brazilian dairy and beef water buffalo herds. as few reports describe the bvdv infection profile the aim of this study was to evaluate the dynamics of bvdv circulation in brazilian dairy water buffalo herds through analysis of the seropositivity rate and the titer range of anti-bvdv neutralizing antibodies in a group of animals that are considered sentinels. blood samples (n = 305) were obtained from ... | 2017 | 29260489 |
| characteristics of yak platelet derived growth factors-alpha gene and expression in brain tissues. | platelet derived growth factors (pdgfs) are key components of autocrine and paracrine signaling, both of which play important roles in mammalian developmental processes. pdgf expression levels also relate to oxygen levels. the characteristics of yak pdgfs, which are indigenous to hypoxic environments, have not been clearly described until the current study. | 2017 | 28553854 |
| partial purification and characterization of glutathione s-transferase from the somatic tissue of gastrothylax crumenifer (trematoda: digenea). | aim of the present study was to carry out the partial purification and biochemical characterization of glutathione s-transferase (gst) from the somatic tissue of ruminal amphistome parasite, gastrothylax crumenifer (gc) infecting indian water buffalo (bubalus bubalis). | 2017 | 29391692 |
| identification of micrornas in corpus luteum of pregnancy in buffalo (bubalus bubalis) by deep sequencing. | this study was aimed to identify mirnas of corpus luteum (cl) in buffaloes during pregnancy. for this study, cl (n=2) were collected from gravid uteri of buffalo and rna was isolated. following this, the purity and integrity of rna was checked and used for deep sequencing using illumina hiseq 2500 platform. the reads' quality was checked prior to in silico analyses viz. identification of conserved, novel and target of mirnas. in this study, out of identified mirnas (3018), 3013 were known and 5 ... | 2017 | 29387104 |
| ethnomedicinal and cultural practices of mammals and birds in the vicinity of river chenab, punjab-pakistan. | although, use of animal species in disease treatment and culture practices is as ancient as that of plant species; however ethnomedicinal uses and cultural values of animal species have rarely been reported. present study is the first report on the medicinal uses of mammals and bird species in pakistan. | 2017 | 28701165 |
| fixed differences in the 3'utr of buffalo prnp gene provide binding sites for mirnas post-transcriptional regulation. | bovine spongiform encephalopathy, a member of transmissible spongiform encephalopathies, has not been reported in buffaloes, bubalus bubalis. prion protein (prp), encoded by the prion protein gene (prnp), is fundamental in the pathogenesis of transmissible spongiform encephalopathies. we previously showed that buffaloes express more prp proteins but lower prnp mrna than cattle in several pivotal tissues like the obex. therefore, we sought to establish whether genetic variability in prnp 3'utr, m ... | 2017 | 28545018 |
| serotyping, antibiotic susceptibility, and virulence genes screening of escherichia coli isolates obtained from diarrheic buffalo calves in egyptian farms. | in egypt as in many other countries, river water buffalo (bubalus bubalis) is considered an important source of high-quality milk and meat supply. the objective of this study was to investigate serotypes, virulence genes, and antibiotic resistance determinants profiles of escherichia coli isolated from buffalo at some places in egypt; noticibly, this issue was not discussed in the country yet. | 2017 | 28831220 |
| serological survey of neospora caninum and toxoplasma gondii in cattle (bos indicus) and water buffaloes (bubalus bubalis) in ten provinces of brazil. | the objective of this study was to determine the prevalence of antibodies to neospora caninum and toxoplasma gondii among 500 cattle (bos indicus) and 500 buffaloes (bubalus bubalis) using the indirect fluorescent antibody test (ifat) technique. blood samples from were collected from water buffalo and cattle in 10 municipalities in the northern region of brazil. the frequency of cattle and water buffaloes seropositive for neospora caninum in pará state, brazil, was 55% and 44%, respectively, and ... | 2017 | 28673459 |
| surveillance for hemorrhagic septicemia in buffalo (bubalus bubalis) as an aid to range expansion of the javan rhinoceros (rhinoceros sondaicus) in ujung kulon national park, indonesia. | the javan rhinoceros (rhinoceros sondaicus) of ujung kulon national park (uknp) is the crown jewel of indonesia's rich natural history. the park lies on a peninsula surrounded by coastline and agriculture-dominated landscapes. the invasion of water buffalo (bubalus bubalis) into the park carries a substantial health risk to the javan rhinoceros and threatens plans to establish a new population outside of its only current range in uknp. hemorrhagic septicemia (hs), known locally as septicemia epi ... | 2017 | 28820298 |
| sarcocystis dehongensis n. sp. (apicomplexa: sarcocystidae) from water buffalo (bubalus bubalis) in china. | water buffalo (bubalus bubalis) is the intermediate host for at least four species of sarcocystis: s. fusiformis, s. buffalonis, s. levinei, and s. sinensis/s. dubeyi. here, a new species, sarcocystis dehongensis, is reported in 51 of 756 (6.7%) water buffaloes in china. by light microscopy, the cysts of s. dehongensis were macroscopic, up to 18.5 mm long and 95 μm in diameter; 4.9-11.9 μm villous protrusions extended beyond the sarcocyst wall. using transmission electron microscopy, the sarcocy ... | 2017 | 28560573 |
| detomidine and butorphanol for standing sedation in a range of zoo-kept ungulate species. | general anesthesia poses risks for larger zoo species, like cardiorespiratory depression, myopathy, and hyperthermia. in ruminants, ruminal bloat and regurgitation of rumen contents with potential aspiration pneumonia are added risks. thus, the use of sedation to perform minor procedures is justified in zoo animals. a combination of detomidine and butorphanol has been routinely used in domestic animals. this drug combination, administered by remote intramuscular injection, can also be applied fo ... | 2017 | 28920785 |
| disposition of a long-acting oxytetracycline formulation in thai swamp buffaloes (bubalus bubalis). | the present study aimed to characterize the pharmacokinetic profile of oxytetracycline long-acting formulation (otc-la) in thai swamp buffaloes, bubalus bubalis, following single intramuscular administration at two dosages of 20 and 30 mg/kg body weight (b.w.). blood samples were collected at assigned times up to 504 h. the plasma concentrations of otc were measured by high-performance liquid chromatography (hplc). the concentrations of otc in the plasma were determined up to 264 h and 432 h aft ... | 2017 | 27597645 |
| brca-1 gene expression and comparative proteomic profile of primordial follicles from young and adult buffalo (bubalus bubalis) ovaries. | in our previous study, we demonstrated that the repair efficiency of dna double-strand breaks declines with increasing age in rat primordial follicles. in the present study, we extended our studies to buffalo (bubalus bubalis) wherein we studied the expression of brca-1 related dna repair genes in primordial follicles of young (12 months-22 months) and adult (72-96 months) buffaloes. the relative expression of selected genes, as determined by rt-pcr, revealed a significant (p < 0.05) decrease in ... | 2017 | 27552356 |
| meat quality of buffaloes finished in traditional or silvopastoral system in the brazilian eastern amazon. | the present study aimed to assess the physical, chemical and sensory characteristics of meat from buffaloes finished in a traditional system (ts) or traditional + silvopastoral system (tsps) with dietary supplementation. crossbred murrah × mediterranean buffaloes were raised from weaning to slaughter in the ts (n = 15) or were raised in the traditional system and finished in the tsps (n = 15). after finishing, animals were slaughtered and their carcasses refrigerated for 24 h. the right side of ... | 2017 | 27448191 |
| biochemical, immunological and kinetic characterization and partial sequence analysis of a thiol proteinase inhibitor from bubalus bubalis kidney: an attempt targeting kidney disorders. | in the present study a thiol proteinase inhibitor was isolated from buffalo kidney making use of ammonium sulphate precipitation and gel filtration chromatography on sephacryl s-100hr column. purified inhibitor is homogeneous as it displayed a single band in gel electrophoresis both under reducing and non-reducing environment and is of 65kda as revealed by gel filtration and sds page. kinetic studies revealed the presence of reversible accompanied with competitive mode of inhibition; showing max ... | 2017 | 26751400 |
| valproic acid assisted reprogramming of fibroblasts for generation of pluripotent stem cells in buffalo (bubalus bubalis). | generation of pluripotent stem cells by reprogramming somatic cells of quality animals has numerous potential applications in agricultural and biomedical sciences. unfortunately, till now, reprogramming of buffalo fetal fibroblast cells (bffs) has been very ineffient despite intensive efforts. here, we attempted to enhance reprogramming efficiency by using the hdac inhibitor valproic acid (vpa) in bffs transfected with plentg-kosm pseudo virus carrying mouse specific pluripotent genes. facs anal ... | 2017 | 27528045 |
| trace elements in raw milk of buffaloes (bubalus bubalis) from campania, italy. | the profile of 18 trace elements was traced in 68 milk samples collected from buffalo farms in the territory known as the "land of fires" in the campania region (italy). this area has been polluted by the illegal dumping in fields of industrial or domestic waste, wich is sometimes then burned spreading toxic contaminants. milk from buffaloes raised on rural farms might be a good indicator of environmental contamination risk in the human food chain. trace element analysis in milk was performed us ... | 2017 | 28530587 |
| retinoic acid induces differentiation of buffalo (bubalus bubalis) embryonic stem cells into germ cells. | development of precise and reproducible culture system for in vitro differentiation of embryonic stem (es) cells into germ cells counts as a major leap forward for understanding not only the remarkable process of gametogenesis, otherwise obscured by limited availability of precursor primordial germ cells (pgcs), but in finally treating the catastrophic infertility. taking into account the significant role of retinoic acid (ra) during in vivo gametogenesis, we designed the present study to invest ... | 2017 | 28526652 |
| a novel polymorphism in the oxytocin receptor encoding gene (oxtr) affects milk fatty acid composition in italian mediterranean river buffalo. | the oxytocin receptor, also known as oxtr, is a protein which functions as receptor for the hormone and neurotransmitter oxytocin and the complex oxytocin-oxytocin receptor plays an important role in the uterus during calving. a characterisation of the river buffalo oxtr gene, amino acid sequences and phylogenetic analysis is presented. the dna regions of the oxtr gene spanning exons 1, 2 and 3 of ten mediterranean river buffalo dna samples were analysed and 7 single nucleotide polymorphisms wer ... | 2017 | 28524009 |
| testicular thermoregulation, scrotal surface temperature patterns and semen quality of water buffalo bulls reared in a tropical climate. | this study evaluated the capacity of thermoregulation and its consequences on the scrotal surface temperature patterns and semen quality of buffalo bulls raised in a wet tropical climate. eleven water buffaloes were evaluated in the rainiest, in the transitional and in the less rainy season. air temperature and humidity were consistently high, but the animals did not show thermal stress in any season. the scrotal temperature gradient of buffalo bulls using infrared thermography was described, an ... | 2017 | 28517368 |
| evaluation of α-linolenic acid for freezability and in vivo fertility of nili ravi (bubalus bubalis) buffalo semen. | alpha linolenic acid (ala) is integral component of cell membrane that protects the cell in stressful events and involves in many metabolic pathways. it was hypothesized that ala have the ability to protect the structural and functional integrity of buffalo spermatozoa during freeze-thawing. therefore, study was designed to evaluate ala supplementation (0, 5, 10 and 20 ng/ml) in extender on freezability and in vivo fertility of buffalo bull spermatozoa. semen from three adult nili-ravi buffalo b ... | 2017 | 28802112 |
| molecular characterization and comparison of phospholipase c zeta (plcz1) gene between swamp (bubalus carabanensis) and riverine (bubalus bubalis) buffaloes: its implications and future perspectives. | phospholipase c zeta, a novel sperm-specific protein which is widely known to induce oocyte activation following fertilization, had already been characterized in various mammalian species, but not in water buffaloes thus far. the present study was conducted to initially characterize and compare the sequences of plcz1 gene of swamp and riverine buffaloes. semen samples were collected; total rna was extracted and reverse-transcribed. plcz1 cdna was then amplified, and submitted for sequencing. buf ... | 2017 | 28799828 |
| influence of season, age and management on scrotal thermal profile in murrah bulls using scrotal infrared digital thermography. | the aim of the present study was to examine the effects of non-genetic factors on scrotal thermographic profile viz., proximal pole temperature (ppt °c), mid pole temperature (mpt °c), distal pole temperature (dpt °c) and ocular temperature (oct) of murrah (bubalus bubalis) breeding bulls. a total of 109 buffalo bulls, maintained at three semen stations (ss), were monitored for scrotal surface and ocular temperatures using infrared thermography twice daily during rainy, winter and summer seasons ... | 2017 | 28791483 |
| effect of supplementation of recombinant regucalcin in extender on cryopreservation of spermatozoa of water buffalo (bubalus bubalis). | elevated intracellular calcium concentration and oxidative damage are two major factors contributing to the poor fertility of cryopreserved spermatozoa. regucalcin (rgn), also known as senescence marker protein-30 (smp-30), is a calcium-binding protein with multiple roles that include calcium homeostasis, anti-oxidative, anti-apoptosis, and anti-proliferation. in drosophila, rgn is reportedly a putative cold-tolerance gene and a cytoprotective role for rgn against intracellular calcium elevation ... | 2017 | 28782859 |
| transcript analysis at dgat1 reveals different mrna profiles in river buffaloes with extreme phenotypes for milk fat. | buffalo dgat1 (diacylglycerol o-acyltransferase 1) was mainly investigated for the characterization of the gene itself and for the identification of the k232a polymorphism, similar to what has been accomplished in cattle, although no information has been reported so far at the mrna level. the importance of dgat1 for lipid metabolism led us to investigate the transcript profiles of lactating buffaloes characterized as high (9.13 ± 0.23) and low (7.94 ± 0.29) for milk fat percentage, and to explor ... | 2017 | 28780112 |
| changes in some blood parameters, milk composition and yield of buffaloes (bubalus bubalis) during the transition period. | in this study changes in hematochemical parameters, milk composition and yield were investigated in buffaloes during the transition period. a total of 93 buffaloes 113.9 ± 8.03 months old and 535 ± 50 kg average body weight were used. parity was recorded, blood samples were collected from 80 days pre-partum until 70 days post-partum; milk samples were collected from 5 days to 70 days post-partum. on serum samples, the values of non-esterified fatty acids, β-hydroxybutyrate, glucose, cholesterol, ... | 2017 | 28776872 |
| genetic perspective of milk yield persistency in the first three lactations of iranian buffaloes (bubalus bubalis). | the objectives of this study were to estimate the genetic parameters and genetic trends for different measures of persistency (pm) for milk yield in iranian buffaloes using a random regression test day model. test day records of the first three lactations of buffaloes within 715 herds comprising 43 014, 38 941 and 33 937 records, respectively, were obtained from the animal breeding center of iran during 1992 to 2012. legendre polynomial functions were chosen to fit the lactation curves in the fr ... | 2017 | 28929983 |
| withdrawn: differential involvement of l- and t-type ca2+ channels, store-operated calcium channel (trpc) and rho-kinase signaling pathway(s) in pgf2α-induced contractions in myometrium of non-pregnant and pregnant buffaloes (bubalus bubalis). | this article has been withdrawn at the request of the author(s) and/or editor. the publisher apologizes for any inconvenience this may cause. the full elsevier policy on article withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal. | 2017 | 28916261 |
| selection of suitable internal control genes for accurate normalization of real-time quantitative pcr data of buffalo (bubalus bubalis) blastocysts produced by scnt and ivf. | we evaluated the suitability of 10 candidate internal control genes (icgs), belonging to different functional classes, namely actb, eef1a1, gapdh, hprt1, hmbs, rps15, rps18, rps23, sdha, and ubc for normalizing the real-time quantitative polymerase chain reaction (qpcr) data of blastocyst-stage buffalo embryos produced by hand-made cloning and in vitro fertilization (ivf). total rna was isolated from three pools, each of cloned and ivf blastocysts (n = 50/pool) for cdna synthesis. two different ... | 2017 | 28880574 |
| anandamide exerts a suppressive effect on sperm binding to oviduct explants through cb1 receptors in the water buffalo (bubalus bubalis). | an endocannabinoid system comprising of anandamide (aea) and its receptor has been shown to play a role in sperm acquisition of fertilizing potential and sperm-oviduct interaction. in the present study, we assessed the effect of sperm pre-treatment with aea or co-incubation of sperm-oviduct explants with aea in the presence or absence of cb1 receptor antagonist (sr141716a) on sperm-oviduct binding in the water buffalo. cryopreserved spermatozoa from 3 murrah buffalo bulls (3 ejaculates from each ... | 2017 | 28869110 |
| the first serological evidence for rift valley fever infection in the camel, goitered gazelle and anatolian water buffaloes in turkey. | rift valley fever (rvf), a vector-borne zoonotic disease, is caused by a phlebovirus (family bunyaviridae). the virus was initially characterized approximately 80 years ago in kenya and disseminated to many countries in the continental africa, saudi arabia, and yemen. the infection has not been reported in turkey. in this study, blood serum samples collected from camel (camelus dromedairus), goitered gazelle (gazella subgutturosa subgutturosa), and buffaloes (bubalus bubalis linneaus) from 2000 ... | 2017 | 28861841 |
| biocomputational identification and validation of novel micrornas predicted from bubaline whole genome shotgun sequences. | micrornas (mirnas) are small (19-25 base long), non-coding rnas that regulate post-transcriptional gene expression by cleaving targeted mrnas in several eukaryotes. the mirnas play vital roles in multiple biological and metabolic processes, including developmental timing, signal transduction, cell maintenance and differentiation, diseases and cancers. experimental identification of micrornas is expensive and lab-intensive. alternatively, computational approaches for predicting putative mirnas fr ... | 2017 | 28844020 |
| morphological studies of the tongue of the egyptian water buffalo (bubalus bubalis) and their lingual papillae adaptation for its feeding habits. | this work was conducted to describe the morphological characters of the tongue of egyptian water buffalo (bubalus bubalis). the lingual root and the dorsal middle region of apex and body in addition to the dorsal and ventral surface of lingual tip were devoided from any fungiform papillae. the lingual tip contains conical papillae only. the ventral surface of lingual apex was divided into two portions by the u-shaped fungiform line into papillary and non-papillary region. histological investigat ... | 2017 | 28833390 |
| analysis of microsatellite markers in a cuban water buffalo breed. | the aim of this regional research communication was to validate a panel of 30 microsatellite markers recommended by fao/isag for studies of biodiversity in cattle to improve the characterisation of cuban buffalo populations. the water buffalo (bubalus bubalis) is an economically important livestock species. therefore, research focused on the study of the genetic relationships among water buffalo populations is useful to support conservation decisions and to design breeding schemes. twenty-eight ... | 2017 | 28831973 |
| comparative therapeutic effect of steroidal and non-steroidal anti-inflammatory drugs on pro-inflammatory cytokine production in water buffalo calves (bubalus bubalis) naturally infected with bronchopneumonia: a randomized clinical trial. | in the current study, we compared the therapeutic effects of a non-steroidal and a steroidal anti-inflammatory drug on the production of pro-inflammatory cytokines, interleukin-1β (il-1β), interleukin-6 (il-6), interleukin-12p40 (il-12p40), interferon gamma (ifnγ), and tumor necrosis factor alpha (tnf-α) in the blood of water buffalo (bubalus bubalis) calves naturally infected by bronchopneumonia. twenty-seven buffalo calves (7 ± 2-month-old, 163 ± 12 kg) reared in smallholder farms in el-dakahl ... | 2017 | 28831754 |
| determination of an optimal membrane-permeable cryoprotectant addition and dilution protocol for water buffalo spermatozoa. | there is a possibility to reduce the toxicity of glycerol and osmotic stress of dmso by lowering their concentrations in freezing extenders. | 2017 | 28767747 |
| posttranslational modifications in spermatozoa and effects on male fertility and sperm viability. | spermatogenesis is a complex and highly regulated process. the ability of spermatozoa to perform its function depends on multiple physiological and genetic factors that are not fully understood. notably, due to lack of transcriptional and translational activity in spermatozoa, posttranslational modifications (ptms) play key roles in determining their viability. ptms not only confer structural changes in the proteome of the spermatozoa cells, but also increase the diversity of the proteome and in ... | 2017 | 28481731 |
| effect of sexual excitation on testosterone and nitric oxide levels of water buffalo bulls (bubalus bubalis) with different categories of sexual behavior and their correlation with each other. | we studied the effect of sexual excitation on serum testosterone and nitric oxide (no) levels in water buffalo bulls with different categories of sexual behavior and their correlation with each other. buffalo bulls were classified according to their sexual behavior (including reaction time, sexual aggressiveness and mating ability): acceptable (good to excellent) (n=5), fair (n=5), and unacceptable (poor) (n=5) sexual behavior. blood samples were collected from all animals immediately before and ... | 2017 | 28477950 |
| ovulatory and fertility response using modified heatsynch and ovsynch protocols in the anovular murrah buffalo (bubalus bubalis). | we studied the effect of modified heatsynch and ovsynch protocols on the ovulatory response (or), estrus induction rate (eir) and conception rate (cr) in the anovular postpartum murrah buffalo (n = 35). in the modified heatsynch protocol (group i; n = 12), buffaloes were given two gnrh at 2 h interval on treatment day 0, pgf (pgf2α) on day 7 and estradiol (e2) 1 mg on day 8. two ftai were done at 20 h intervals after e2 administration. in the modified ovsynch protocol (group ii; n = 15), gnrh wa ... | 2017 | 28460685 |
| plasma proteome of buffaloes. | the proteomic approach has aroused the interest of veterinary medicine researchers, especially regarding the production of biopharmaceuticals and diagnosis of diseases in farm animals. water buffaloes have gained prominence in the world economy due to the quality of their milk, meat, and leather, in addition to being an important donor of blood components. this work aimed to identify and characterize the proteins present in the blood plasma of murrah buffaloes (bubalus bubalis) through 2d electr ... | 2017 | 28452126 |
| mastitis outcomes on pre-ovulatory follicle diameter, estradiol concentrations, subsequent luteal profiles and conception rate in buffaloes. | the objectives of this study was to investigate the outcome of mastitis, in its clinical or subclinical forms, on the mean diameter of pre-ovulatory follicle (pof), plasma estradiol concentration on the day of estrus, subsequent luteal profile and subsequent conception rate in buffaloes. sixty dairy buffalo (bubalus bubalus) conducted in this study were divided into three groups {healthy (h), n=20; subclinical mastitis (scm), n=18; and clinical mastitis (cm), n=22}. ultrasonography of ovaries re ... | 2017 | 28442176 |
| buffalo leukemia inhibitory factor induces differentiation and dome-like secondary structures in cos-1 cells. | this study aimed to understand the molecular characteristics of buffalo leukemia inhibitory factor (bulif) and the generation of a stably transfected cos-1_bulif cell line for its functional characterization. cumulus cells, isolated from oocytes, were separated, and total cdna was prepared. the bulif gene was ligated into the cloning vector pjet1.2/blunt and expression vector pacgfp-n1 which was transfected into cos-1 cells and confirmed by qrt-pcr and western blot. bulif was immunoprecipitated ... | 2017 | 28441662 |
| characterization of leukocyte subsets in buffalo (bubalus bubalis) with cross-reactive monoclonal antibodies specific for bovine mhc class i and class ii molecules and leukocyte differentiation molecules. | although buffaloes (bubalus bubalis) are a major component of the livestock industry worldwide, limited progress has been made in the study of the mechanisms regulating the immune response to pathogens and parasites affecting their health and productivity. this has been, in part, attributable to the limited availability of reagents to study immune responses in buffalo. as reported here, a set of cross-reactive monoclonal antibodies (mabs), developed against bovine, ovine and caprine leukocyte di ... | 2017 | 28433527 |
| intermolecular interactions between dmα and dmβ proteins in bula-dm complex of water buffalo bubalus bubalis. | the major histocompatibility complex (mhc) with its three classes represents a cluster of tightly linked genes with defined immunological and non-immunological functions. the dm, a mhc class ii molecule is formed by the non-covalent association of dmα and dmβ chains. it binds with the processed peptide antigens and presents them to t lymphocytes, thereby triggering the immune responses. startlingly, the expression pattern and structural organization of dmα and dmβ proteins in buffalo remains und ... | 2017 | 28419545 |
| seroepidemiology of infection withneospora caninum, leptospira, and bovine herpesvirus type 1 in water buffaloes(bubalus bubalis)in veracruz, mexico. | we aimed to determine the seroprevalence of infection withneospora caninum, leptospira, and bovine herpesvirus type 1 and risk factors associated with these infections in water buffaloes in veracruz state, mexico. through a cross-sectional study, 144 water buffaloes(bubalus bubalis)raised in 5 ranches of veracruz were examined for anti-n.caninumand anti-bovine herpesvirus type 1 antibodies by enzyme immunoassays, andanti-leptospira interrogansantibodies by microscopic agglutination test. of the ... | 2017 | 29403656 |
| role of bcl2l10 in regulating buffalo (bubalus bubalis) oocyte maturation. | it has been reported that bcl2l10 is abundantly and specifically expressed in adult human and mouse oocytes and played a very important role in oocytes maturation and early embryonic development. this study is to investigate the expression pattern of bcl2l10 in buffalo ovaries and its effect on the in vitro maturation of buffalo oocytes, so as to dissect mechanism of oocytes maturation and provide theoretical guidance for improvement of the in vitro maturation of buffalo oocytes. the results sho ... | 2017 | 29331495 |
| cytochrome b conservation between six camel breeds reared in egypt. | this study was aimed to assess cytochrome b conservation in six breeds of camels reared in egypt and to compare its sequence with those of other livestock species. the 208-bp fragments from camel mtdna cyto b were amplified using pcr for 54 camels belonging to 6 camel breeds reared in egypt. the alignment of camel cyto b sequences showed the presence of two polymorphic sites resulting in four haplotypes and their nucleotide sequences were submitted to genbank under the accession numbers: kx90989 ... | 2017 | 30647635 |
| ultrasonography as a diagnostic tool in egyptian buffaloes (bubalus bubalis) with traumatic pericarditis. | traumatic pericarditis (tp) remains a serious problem facing bovine producers; particularly in the developing countries; causing severe economic losses. this study was carried out on 47 buffaloes including; control buffaloes (n = 20) and buffaloes with tp (n = 27) at assiut governorate, egypt. all animals were subjected to thorough clinical examination, whole blood profiling, blood serum biochemical assays, and radiographic and ultrasonographic examinations. the recorded ultrasonographic finding ... | 2017 | 30255066 |
| morphological and molecular identification of explanatum explanatum in domestic water buffalo in pakistan. | more than 70 species of the family paramphistomatidae, have been identified in ruminants in different parts of the world. most are pathogenic, causing amphistomosis. adult flukes of this family have a predilection for the rumen, liver or bile duct of ruminants where they may cause damage to the epithelium. identification of adult paramphistomes to the species level based on morphology alone requires specialized knowledge, whereas, molecular genetic marker analysis is more precise and transferabl ... | 2017 | 31014638 |
| modulation of immunity and inflammatory gene expression in buffalo (bubalus bubalis) with some digestive disorders. | so far, there has been scarce information about the status of immunoglobulins (ig) and the gene expression of inflammatory cytokines in buffaloes showing digestive troubles. the purpose of the present study was to explore the modulation of gene expression of some immune-inflammatory markers in buffaloes suffered from various digestive disorders. for this reason, 50 native breed water buffaloes were studied. forty of these buffaloes showed various symptoms of digestive disorders and were allocate ... | 2017 | 32214977 |
| first study of sperm mediated gene transfer in egyptian river buffalo. | the present study was carried out to find the best treatments for enhancing the ration of insertion of a desired gene construct (pegfp-n1) onto the sperm of buffalo as the first step for the production of transgenic buffalo using sperm mediated gene transfer (smgt). the tested conditions were plasmid dna concentration, sperm concentration, transfecting agent concentration: dimethyle sulphoxide (dmso) and time of transfection. the study proved that the best conditions for producing transgenic emb ... | 2017 | 30647689 |
| elucidating the genetic basis of an oligogenic birth defect using whole genome sequence data in a non-model organism, bubalus bubalis. | recent strong selection for dairy traits in water buffalo has been associated with higher levels of inbreeding, leading to an increase in the prevalence of genetic diseases such as transverse hemimelia (th), a congenital developmental abnormality characterized by absence of a variable distal portion of the hindlimbs. limited genomic resources available for water buffalo required an original approach to identify genetic variants associated with the disease. the genomes of 4 bilateral and 7 unilat ... | 2017 | 28045068 |
| the apicoplast genomes of two taxonomic units of babesia from sheep. | the apicoplast (ap) is a unique, non-photosynthetic organelle found in most apicomplexan parasites. due to the essential roles that this organelle has, it has been widely considered as target for drugs against diseases caused by apicomplexans. exploring the ap genomes of such parasites would provide a better understanding of their systematics and their basic molecular biology for therapeutics. however, there is limited information available on the ap genomes of apicomplexan parasites. in the pre ... | 2017 | 27916258 |
| a serosurvey for ruminant pestivirus exposure conducted using cattle sera collected for brucellosis surveillance in the united states. | four species of ruminant pestivirus are currently circulating in the united states: bovine viral diarrhea virus 1 and 2 (bvdv-1, -2; predominant host: cattle), border disease virus (bdv; predominant host: sheep), and pronghorn virus (sporadically detected in wild ruminants). a third bovine pestivirus called hobi-like virus has been detected in cattle and water buffalo in south america, asia, and europe. to date, no isolations of hobi-like viruses from u.s. cattle have been reported. to assess ex ... | 2017 | 28074709 |
| genome sequence of bubaline alphaherpesvirus 1 (buhv1) isolated in australia in 1972. | bubaline alphaherpesvirus 1 (buhv1) is a member of the family herpesviridae, subfamily alphaherpesvirinae, genus varicellovirus. to date, no full genome sequence of buhv has been published. here, we report the complete genome sequence of bubaline alphaherpesvirus 1 (buhv1) strain b6 (buhv1-b6), isolated from a water buffalo (bubalus bubalis) in 1972 in australia. the virus was multiplied in mdbk cells, and the dna was extracted and subjected to high-throughput sequencing. the reads were aligned ... | 2017 | 28063080 |
| characterization of a recombinant newcastle disease virus expressing the glycoprotein of bovine ephemeral fever virus. | bovine ephemeral fever (bef) is caused by the arthropod-borne bovine ephemeral fever virus (befv), which is a member of the family rhabdoviridae and the genus ephemerovirus. befv causes an acute febrile infection in cattle and water buffalo. in this study, a recombinant newcastle disease virus (ndv) expressing the glycoprotein (g) of befv (rl-befv-g) was constructed, and its biological characteristics in vitro and in vivo, pathogenicity, and immune response in mice and cattle were evaluated. bef ... | 2017 | 27757685 |
| detection of pseudocowpox virus in water buffalo (bubalus bubalis) with vesicular disease in the state of são paulo, brazil, in 2016. | parapoxviruses are zoonotic viruses that infect cattle, goats and sheep; there have also been reports of infections in camels, domestic cats and seals. | 2017 | 27774853 |
| origins and global context of brucella abortus in italy. | brucellosis is a common and chronic disease of cattle and other bovids that often causes reproductive disorders. natural infection in cattle is caused by brucella abortus and transmission typically occurs during abortions, calving, or nursing. brucellosis is also a major zoonotic disease due to contamination of dairy products or contact with the tissues of infected animals. brucellosis has been eradicated from most of the developed world in the last 40 years but persists in many regions-the dise ... | 2017 | 28152976 |
| investigation of the prion protein gene (prnp) polymorphisms in anatolian, murrah, and crossbred water buffaloes (bubalus bubalis). | bovine spongiform encephalopathy (bse) of the cattle is the outstanding disease among other transmissible spongiform encephalopathy (tses). it can be transmitted from the cattle to a human and causes a new variant of the creutzfeldt-jakob disease (cjd). it is known that prion protein coding gene (prnp) plays a major role in the tse susceptibility or resistance in some species. recent researches demonstrated that the insertion (in) and deletion (del) polymorphisms within promoter and intron 1 reg ... | 2017 | 27822596 |
| development of an indirect elisa using different fragments of recombinant ncgra7 for detection of neospora caninum infection in cattle and water buffalo. | dense granules are immunodominant proteins for the standardization of immunodiagnostic procedures to detect neosporosis. in the presented study different fragment of a dense-granule protein was evaluated for serodiagnosis of neospora caninum in cattle and water buffalo. | 2017 | 25904948 |
| transcriptomic responses of water buffalo liver to infection with the digenetic fluke fasciola gigantica. | fasciola gigantica, the tropical liver fluke, infects buffaloes in asian and african countries and causes significant economic losses and poses public health threat in these countries. however, little is known of the transcriptional response of buffaloes to infection with f. gigantica. the objective of the present study was to perform the first transcriptomic analysis of buffalo liver infected by f. gigantica. understanding the mechanisms that underpin f. gigantica infection in buffaloes will co ... | 2017 | 28143561 |
| pharmacokinetics of amoxicillin trihydrate in thai swamp buffaloes (bubalus bubalis): a pilot study. | this study aimed to investigate the pharmacokinetic characteristics of amoxicillin (amx) in thai swamp buffaloes, bubalus bubalis, following single intramuscular administration at two dosages of 10 and 20 mg/kg body weight (b.w.). blood samples were collected at assigned times up to 48 h. the plasma concentrations of amx were measured by liquid chromatography-tandem mass spectrometry (lc-ms/ms). the concentrations of amx in the plasma were determined up to 24 h after i.m. administration at both ... | 2017 | 27292774 |
| genetic characterization of ovine herpesvirus 2 strains involved in water buffaloes malignant catarrhal fever outbreaks in southern italy. | ovine herpesvirus 2 (ovhv-2) was responsible for two outbreaks of malignant catarrhal fever (mcf) on two water buffalo farms in southern italy. in this study, the presence of this virus in the nasal swabs from sick animals as well as in the organs of dead buffaloes was ascertained by a real-time pcr assay. positive samples also underwent a relative quantitative analysis of the viral dna in them. all the dead animals had the highest relative viral quantities, while buffaloes recovering from the v ... | 2017 | 28110782 |
| efficacy of a polyvalent mastitis vaccine against staphylococcus aureus on a dairy mediterranean buffalo farm: results of two clinical field trials. | in the last years the knowledges on mediterranean buffalo (mb) mastitis is remarkably improving, nevertheless the attention has been never focused on vaccination as preventive strategy for the control of mastitis in these ruminates. the aim of the current study was to assess clinical efficacy over time of two different preventive vaccination protocols against s. aureus mastitis, in primiparous mb.vaccinated (vg) and not-vaccinated (n-vg) groups, of 30 mb each one, were selected from two differen ... | 2017 | 28103866 |
| molecular characterisation of theileria orientalis in imported and native bovines from pakistan. | the epidemiological aspects of theileria orientalis in pakistan are unknown; therefore, investigations using sensitive and precise molecular techniques are required. this study reports the first molecular characterisation of t. orientalis detected from imported (bos taurus) and native cattle (bos indicus×bos taurus) and buffaloes (bubalus bubalis) selected from four districts of punjab, pakistan. dna samples from blood (n=246) were extracted and tested using conventional pcr utilising the major ... | 2017 | 27838527 |
| cryopreservation of nili-ravi buffalo bull sperm in cryodiluent supplemented with lolium perenne protein preparations. | semen from the nili-ravi buffalo bull, bubalus bubalis, shows poor survival after freeze storage compared to bovine (bos taurus and bos indicus) semen. freeze-susceptibility distinctions in these two genera have been attributed to differences in sperm membranes. | 2017 | 28376139 |
| differential expression of isg 15 mrna in peripheral blood mononuclear cells of nulliparous and multiparous pregnant versus non-pregnant bos indicus cattle. | embryonic mortality is found to be the main source of reproductive wastage in domestic ruminants. many genes are involved in the growth and development of the embryo, and the interferon-stimulated gene 15 (isg 15) is one of the major gene stimulated by interferon tau, the maternal recognition of pregnancy signal in ruminants. in this study, both genomic and cdna sequences of isg 15 from bos indicus (deoni breed) were amplified and characterized. the genomic sequence of deoni isg 15 exhibited 99% ... | 2017 | 27766692 |
| challenges for beef production in smallholder communities with low reproductive management skills: a case study from northern lao pdr. | improved large ruminant productivity is increasingly acknowledged as a pathway for the alleviation of rural poverty and food insecurity in smallholder communities in southeast asia; yet, in much of laos, bovine reproductive management is practically absent. large ruminant reproduction skills were studied, using face-to-face surveys (n=60) of the knowledge, attitudes, and practices (kap) of farmers, plus an extension of an examination of parameters of reproductive efficiency (n = 1786 cattle and ... | 2017 | 27718106 |
| lumpy skin disease in cattle: frequency of occurrence in a dairy farm and a preliminary assessment of its possible impact on egyptian buffaloes. | lumpy skin disease (lsd) is an endemic infectious disease of cattle in egypt. this survey aimed to define the prevalence of clinical and sub-clinical lsd virus (lsdv) infection among cattle and investigate their contact with water buffaloes (bubalus bubalis) in order to improve the understanding of lsd epidemiology. cattle and buffalo were examined owing to the appearance of skin lesions. because clinical signs were consistent with lsdv infection, samples from cattle in a non-grazing dairy farm ... | 2017 | 28397517 |
| complement component 3: characterization and association with mastitis resistance in egyptian water buffalo and cattle. | mastitis is an infectious disease of the mammary gland that leads to reduced milk production and change in milk composition. complement component c3 plays a major role as a central molecule of the complement cascade involving in killing of microorganisms, either directly or in cooperation with phagocytic cells. c3 cdna were isolated, from egyptian buffalo and cattle, sequenced and characterized. the c3 cdna sequences of buffalo and cattle consist of 5025 and 5019 bp, respectively. buffalo and ca ... | 2017 | 28360391 |
| molecular cloning, identification, and expression patterns of myostatin gene in water buffalo (bubalus bubalis). | myostatin (mstn), also named growth differentiation factor 8 (gdf8), is a transforming growth factor-β (tgf-β) family member with a key role in the negative regulation of skeletal muscle growth. however, its role in ovarian folliculogenesis remains unclear. to provide us with a basis for understanding this role, we cloned mstn and examined its expression patterns in water buffalo (bubalus bubalis). the complete orf of the water buffalo mstn gene is 1,128 nucleotides, which encode a 375 amino aci ... | 2017 | 28355117 |
| identification of single nucleotide polymorphism in protein phosphatase 1 regulatory subunit 11 gene in murrah bulls. | this study was conducted with the objective to identify single nucleotide polymorphism (snp) in protein phosphatase 1 regulatory subunit 11 (ppp1r11) gene in murrah bulls. | 2017 | 28344410 |
| comparative study of the gut microbiome potentially related to milk protein in murrah buffaloes (bubalus bubalis) and chinese holstein cattle. | previous studies suggested a close relationship between ruminant gut microbes and the mammary gland. in this study, shotgun metagenomic sequencing was used to reveal the differences in the intestinal microbiome potentially related to milk components in murrah buffaloes and chinese holstein cattle. a pcoa based on the weighted unifrac distances showed an apparent clustering pattern in the structure of intestinal microbiota between buffalo and cattle. we could attribute the structural difference t ... | 2017 | 28176851 |