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Antifungal activity and chemical composition of essential oils from Smyrnium olusatrum L. (Apiaceae) from Italy and Portugal.The essential oils and supercritical CO(2) extracts of wild Smyrnium olusatrum L. growing in Sardinia (Italy) and in Portugal were investigated. For the study, oils were isolated from total plant aerial part (umbels containing seeds). The content of ß-phellandrene (67.3% vs. 42.7%) and a-pinene (31.9% vs. 1.2%), respectively, the main components of Portuguese and Italian essential oils, declined during the maturation stage of the umbels. Contrarily, some other important components, particularly ...201121902563
Decreasing Prevalence of Microsporum canis Infection in Korea: Through Analysis of 944 Cases (1993-2009) and Review of Our Previous Data (1975-1992).Since 1957, Microsporum (M.) canis has been one of the common causative agents of dermatophytosis in Korea. We analyzed 944 patients infected with M. canis who attended outpatient clinic over a 17-year period (1993-2009). M. canis infections were diagnosed by mycological examinations, including direct microscopic examinations with 15% KOH and cultures on potato dextrose agar complemented with 0.5% chloramphenicol. Mycological examinations confirmed 164,903 cases of dermatophytosis, 944 (0.6%) of ...201122187358
design, synthesis and antifungal activity of some new imidazole and triazole derivatives.triazole and imidazole are incorporated into the structures of many antifungal compounds. in this study a novel series of 1,2,4-triazole, imidazole, benzoimidazole, and benzotriazole derivatives was designed as inhibitors of cytochrome p450 14α-demethylase (14dm). these structures were docked into the active site of mt-cyp51, using autodock program. sixteen compounds with the best binding energy were synthesized. the chemical structures of the new compounds were confirmed by elemental and spectr ...201121984016
changes of dermatophytoses in southwestern greece: an 18-year survey.the isolation and distribution rate of dermatophytes as causative agents of superficial mycoses of skin, hair, and nails during an 18-year period (1991-2008) at a university hospital are presented. a comparative analysis of epidemiological differences within the first (1991-1999) and the second 9-year period (2000-2008) was performed. skin scrapings, nail, and hair specimens were examined by a direct microscopic examination and culture. identification of dermatophyte species was based on macrosc ...201121331751
clinico-epidemiological and mycological aspects of tinea incognito in iran: a 16-year study.tinea incognito is a dermatophytic infection in which topical or systemic steroids have modified the clinical appearance of the mycosis and mimicking other skin diseases.201121441710
neonatal dermatophytosis: report of a case and review of the literature.tinea capitis is a common fungal infection in children but rare among neonates. we present a case of a 3-week-old infant with multiple scalp lesions for 1 week. a diagnosis of tinea capitis due to microsporum canis was made based on clinical morphology, wood's lamp, and biopsy with a positive pas stain. she was effectively treated with oral griseofulvin and ketoconazole shampoo.201121504448
mycetoma of the scalp due to microsporum canis: hystopathologic, mycologic, and immunogenetic features in a 6-year-old girl.dermatophytic mycetoma is an extremely rare subcutaneous mycosis. here, we report the case of a 6-year-old girl with clinical, histologic, and mycologic findings consistent with a mycetoma of the scalp caused by microsporum canis. to our knowledge, this is the first report showing the immunologic and immunogenetic features of a patient with a recalcitrant dermatophytic mycetoma.201121513850
synthesis, characterization and biological studies of sulfonamide schiff's bases and some of their metal derivatives.a new series of schiff base ligands derived from sulfonamide and their metal(ii) complexes [cobalt(ii), copper(ii), nickel(ii) and zinc(ii)] have been synthesized and characterized. the nature of bonding and structure of all the synthesized compounds has been explored by physical, analytical and spectral data of the ligands and their metal(ii) complexes. the authors suggest that all the prepared complexes possess an octahedral geometry. the ligands and metal(ii) complexes have been screened for ...201121534864
cluster analysis of microsporum canis isolated from a patient with tinea corporis and an infected cat based on the dna sequences of nuclear ribosomal internal transcribed spacer 1. 201121615535
analysis of 25 cases of microsporum canis infection encountered at a dermatology clinic in kumamoto during a recent 3-year period.twenty-five cases of dermatophytoses caused by microsporum canis were encountered during a 3-year period (january 2008-december 2010). their diagnosis was based on detection of fungal elements by direct microscopy and identification of m. canis by fungal culture. there were 17 women and 8 men ; they ranged in age from 4 to 85 years (mean, 34.6). the affected site was the head (n=5), face (n=8), neck (n=5), arm (n=8), leg (n=5), and trunk (n=5) ; exposed sites were those most often affected. a lo ...201121788725
[scalp ringworm tinea capitis in tunisian infants.]objective: the aim of the study was to specify the epidemiological, clinical, and mycological particularities of tinea capitis in infants. design: we retrospectively collected data from the files of 245 infants presenting with tinea capitis, followed in the hedi-chaker hospital dermatology department and in two mycology laboratories of the sfax hospital, between january 1995 and december 2006. we collected the epidemiological, clinical, and mycological data for each patient. results: we included ...201121764534
molecular identification and phylogenesis of dermatophytes isolated from rabbit farms and rabbit farm workers.little information is available on the molecular epidemiology of dermatophytoses in rabbit farms and farm workers. a total of 117 isolates belonging to the trichophyton mentagrophytes complex and 21 isolates of microsporum canis were collected from rabbits with or without skin lesions, air samples of farms known to harbour these pathogens, and from farm workers with skin lesions, and molecularly characterized. sequencing of amplicons from the t. mentagrophytes complex and m. canis isolates revea ...201121840652
mechanisms of skin adherence and invasion by dermatophytes.dermatophytes are keratinophilic fungi that can be pathogenic for humans and animals by infecting the stratum corneum, nails, claws or hair. the first infection step consists of adherence of arthroconidia to the stratum corneum. the mechanisms and the kinetics of adherence have been investigated using different in vitro and ex vivo experimental models, most notably showing the role of a secreted serine protease from microsporum canis in fungal adherence to feline corneocytes. after germination o ...201121831104
ganoderma lucidum: a source for novel bioactive lectin.ganoderma lucidum is known for its high medicinal value, clinically used in treatment for various diseases. we have selected this mushroom for isolation of novel bioactive lectin. the isolation procedure comprised of ion-exchange chromatography on deae- cellulose and affinity chromatography on affi-gel blue gel. purified lectin was monomer with a molecular mass of 15 kda, determined by sds-page, gel filtration, maldi-tof. it showed hemagglutinating activity against both human and animal erythroc ...201121728991
Zoonoses of procyonids and nondomestic felids.There are several important zoonotic diseases which can be acquired from procyonids, and nondomestic felids. Baylisascaris procyonis, the raccoon roundworm, is a common parasite of raccoons and can cause visceral, ocular, or neural larval migrans in people. Neural larval migrans can cause severe signs in individuals. Dermatophytosis and enteric pathogens are the most important zoonotic agents found in nondomestic felids. Microsporum canis infections can be spread from nondomestic felids to owner ...201121872788
strains differentiation of microsporum canis by rapd analysis using (gaca)4 and (aca)5 primers.molecular analysis of dermatophytes (based on pcr fingerprinting) revealed high clonal differentiation between the genus and species. microsporum canis (zoophilic dermatophyte, belonging to genus microsporum), responsible for most cases of tinea capitis in children, tinea corporis in adults and dermatophytoses in cats, is very unique in comparison with other dermatophytes. results of most molecular studies show that there is no clonal differentiation within m. canis as distinct from other specie ...201121905632
preparation and evaluation of dermal delivery system of griseofulvin containing vitamin e-tpgs as penetration enhancer.griseofulvin, an antifungal agent, is a bcs class ii drug slowly, erratically, and incompletely absorbed from the gastrointestinal tract in humans. the clinical failure of the conventional oral therapy of griseofulvin is most likely attributed to its poor solubility and appreciable inter- and intra-subject variation in bioavailability from different commercial products. moreover, the conventional oral therapy is associated with numerous adverse effects and interactions with other drugs. the purp ...201122130790
Difference in keratinase activity of dermatophytes at different environmental conditions is an attribute of adaptation to parasitism.Dermatophytes are a group of morphologically and physiologically related moulds, which cause well-defined infection called dermatophytosis. The enzymatic ability of fungi to decompose keratin has long been interpreted as a key innovation in the evolution of animal dermatology. In the present study, keratinase activity profile among Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum canis and Microsporum gypseum isolated on keratin substrates such as human hair, human nail and chicken ...201122032519
a 30-year survey of paediatric tinea capitis in southern spain.tinea capitis (tc) is the most common mycological infection in childhood. the epidemiology of tc varies depending on the geographical area and the time of study, and although it is an important public health problem in our area, only a few long-term studies have focused on children.201120553362
use of itraconazole and either lime sulphur or malaseb concentrate rinse ® to treat shelter cats naturally infected with microsporum canis: an open field trial.in an open non-randomized study, 90 cats with severe dermatophytosis were treated with 21 days of oral itraconazole at 10 mg/kg and one of three topical antifungal rinses applied twice weekly: lime sulphur (lso); reformulated lime sulphur with an odour-masking agent (lsr); or a 0.2% miconazole nitrate and 0.2% chlorhexidine gluconate rinse (mc). weekly examinations and fungal cultures were used to monitor the cats' response to therapy. if at day 42 of treatment cats were still strongly fungal cu ...201120604908
[fungi, pets and their owners].some parasitic or pathogen fungi for pet skin are possibly transmitted to humans in whom they are responsible for superficial mycozoonoses. cats, dogs and some small rodents are commonly involved. the lesions correspond to glabrous skin dermatophytoses and to microsporic tinea of the scalp. these disorders represent the vast majority of the prevalent mycozoonoses in wallonia. microsporum canis and trichophyton mentagrophytes are the two fungi that are commonly involved.201122216732
antifungal activity of aegle marmelos (l.) correa (rutaceae) leaf extract on dermatophytes.to evaluate the in vitro antifungal activity of aegle marmelos leaf extracts and fractions on the clinical isolates of dermatophytic fungi like trichophyton mentagrophytes, trichophyton rubrum, microsporum canis, microsporum gypseum and epidermophyton floccosum.201123569781
tinea capitis mimicking folliculitis decalvans.we report on an adult patient with tinea capitis caused by microsporum canis, who presented with diffuse alopecia and follicular pustules, mimicking folliculitis decalvans. examination of the scalp showed severe alopecia with prominent involvement of the frontal and vertex scalp: the skin was markedly erythematous with pustules and brownish crusts. videodermoscopy revealed visible follicular ostia, numerous pustular lesions and several comma hairs. fluconazole 150 mg a week for 8 weeks associate ...201119638002
direct detection of five common dermatophyte species in clinical samples using a rapid and sensitive 24-h pcr-elisa technique open to protocol transfer.identification of dermatophytes is usually based on morphological characteristics determined by time-consuming microscopic and cultural examinations. an effective pcr-elisa method has been developed for rapid detection of dermatophyte species directly from clinical specimens within 24 h. isolated genomic dna of skin scrapings and nail samples from patients with suspected dermatophyte infections is amplified with species-specific digoxigenin-labelled primers targeting the topoisomerase ii gene. t ...201119793206
prevalence of tinea capitis in southeastern austria between 1985 and 2008: up-to-date picture of the current situation.tinea capitis is the most common dermatophyte infection in childhood, but may rarely occur in adults and the elderly. causative agents vary within different geographical areas as well as during decades. the aim of this study was to evaluate the prevalence and causative agents of tinea capitis in southeastern austria. retrospective analysis of 714 patients diagnosed with tinea capitis seen at the outpatient department of dermatology/medical university of graz during the time period 1985-2008 was ...201119863725
use of western blot to study microsporum canis antigenic proteins in canine dermatophytosis.western blotting was used to describe the microsporum canis proteins with antigenic activity in dogs with dermatophytosis. electrophoretic separation of whole fungal strain extract cultured from a cat was performed under denaturing conditions. the proteins were blotted onto nitrocellulose and probed with sera collected from 22 dogs with dermatophytosis (18 m. canis, 3 m. gypseum, 1 trichophyton mentagrophytes; group a), 20 dogs with skin diseases other than dermatophytosis, and 22 dogs with no c ...201119912544
a 5-year survey of dermatomycoses in southwest poland, years 2003-2007.the study was performed to analyse the spectrum of dermatomycoses in southwest poland during the period 2003-2007. a total of 10,486 patients were investigated for fungal skin infections by means of native specimen and cultivating procedures. skin scrapings, plucked hairs and nail clippings were examined and identified by direct microscopy and culture. from 2468 patients, 2753 fungi were identified including dermatophytes, yeast and moulds. among the dermatophytes, the most common pathogen isola ...201119925570
[epidemiology of tinea capitis in outpatients at the children's hospital in rabat (morocco)].the aims of this report on results of a retrospective study were to identify the incidence of this pathology among outpatients at the children's hospital in rabat, morocco, the species involved, their respective prevalence, and the influence of the age and sex of the patient.201124451494
design, synthesis, and antifungal activity of new α-aminophosphonates.α-aminophosphonates are bioisosteres of amino acids and have several pharmacological activities. these compounds have been synthesized by various routes from reaction between amine, aldehyde, and phosphite compounds. in order to synthesize α-aminophosphonates, catalytic effect of cucl2 was compared with fecl3. also all designed structures as well as griseofulvin were docked into the active site of microtubule (1jff), using autodock program. the results showed that the reactions were carried out ...201125954522
alkylphenol activity against candida spp. and microsporum canis: a focus on the antifungal activity of thymol, eugenol and o-methyl derivatives.in recent years there has been an increasing search for new antifungal compounds due to the side effects of conventional antifungal drugs and fungal resistance. the aims of this study were to test in vitro the activity of thymol, eugenol, estragole and anethole and some o-methyl-derivatives (methylthymol and methyleugenol) against candida spp. and microsporum canis. the broth microdilution method was used to determine the minimum inhibitory concentration (mic). the minimum fungicidal concentrati ...201125134762
the use of ozonized oil in the treatment of dermatophitosis caused by microsporum canis in rabbits.the ozone is effective against most microorganisms due to its high oxidant power. low concentrations and short-term contact are sufficient to inactivate bacteria, mold, yeast, parasites, seaweeds, protozoa and fungi. microsporum canis is an important agent of dermatophitosis in human and animal. the aim of the current study was to assess the efficacy of ozonized oil over microsporum canis in rabbits. eighteen male new zealand white rabbits, weight ranging from 2 to 3.2 kg were depilated in the c ...201124031632
tinea capitis in southeastern china: a 16-year survey.this survey was a retrospective of a 16-year (1993-2008) study on the incidence, clinical features, and etiological agents of tinea capitis mainly representing the southeastern china. the diagnosis was confirmed by direct microscopic examination. eight hundred and sixty-six patients with tinea capitis, 381 males (44%) and 485 females (56%), were enrolled in this study. patients were between 20 days and 84 years old with an average of 10.5 years and the peak incidence was in the age group of 6-10 ...201019936963
secreted subtilisin sub3 from microsporum canis is required for adherence to but not for invasion of the epidermis.microsporum canis is a pathogenic dermatophyte that causes a superficial cutaneous mycosis, mainly in cats and humans. proteolytic enzymes, including subtilisins, have been postulated to be key factors involved in adherence and invasion of the stratum corneum and keratinized epidermal structures.201019995373
identification of antibacterial and antifungal pharmacophore sites for potent bacteria and fungi inhibition: indolenyl sulfonamide derivatives.synthesis of seven new indolenyl sulfonamides, have been prepared by the condensation reaction of indole-3-carboxaldehyde with different sulfonamides such as, sulphanilamide, sulfaguanidine, sulfathiazole, sulfamethoxazole, sulfisoxazole, sulfadiazine and sulfamethazine. these synthesized compounds have been used as potential ligands for complexation with some selective divalent transition metal ions (cobalt, copper, nickel & zinc). structure of the synthesized ligands has been deduced from thei ...201020005022
synthesis, structure, and antifungal evaluation of some novel 1,2,4-triazolylmercaptoacetylthiosemicarbazide and 1,2,4-triazolylmercaptomethyl-1,3,4-thiadiazole analogs.novel 1-[[4-(4-bromophenyl)-5-(2-furyl)-4h-1,2,4-triazole-3-yl]mercaptoacetyl]-4-alkyl/aryl-3-thiosemicarbazides (5-12) were synthesized by the reaction of 4-(4-bromophenyl)-5-(2-furyl)-4h-1,2,4-triazole-3-ylmercaptoacetylhydrazide (4) with substituted isothiocyanates. cyclodehydration of thiosemicarbazides with concentrated sulfuric acid yielded 2-[4-(4-bromophenyl)-5-(2-furyl)-4h-1,2,4-triazole-3-yl]mercaptomethyl-5-alkyl/arylamino-1,3, 4-thiadiazoles (13-17). the new compounds were evaluated ...201020030516
in vitro antimicrobial activity of four ficus carica latex fractions against resistant human pathogens (antimicrobial activity of ficus carica latex).methanolic, hexanoïc, chloroformic and ethyl acetate extracts of ficus carica latex were investigated for their in vitro antimicrobial proprieties against five bacteria species and seven strains of fungi. the green fruit latex was collected from chott mariam souse, middle east coast of tunisia. the antimicrobial activity of the extracts was evaluated and based respectively on the inhibition zone using the disc-diffusion assay, minimal inhibition concentration (mic) for bacterial testing and the ...201020067867
the spectrum of fungal infections with a special reference to dermatophytoses in the capital area of kuwait during 2000-2005: a retrospective analysis.fungal infections constitute a major health problem all over the world. signs and symptoms induced by various dermatophytic infections are difficult to distinguish clinically from each other. so, characterization by in vitro culture is required for appropriate diagnosis and treatment as well as to study the epidemiological characteristics in a region.201019921463
dynamics of dermatophytosis frequency in mexico: an analysis of 2084 cases.we analysed 15,101 biological samples from patients presenting with superficial mycoses who attended outpatient services over a 10-year period. scale samples were processed for direct microscopic examination with 15% koh and cultured on sabouraud glucose agar plus chloramphenicol and cycloheximide. laboratory examination confirmed 4,709 cases of superficial mycosis (31.18%), of which 2,084 (44.26%) were dermatophytoses. the species most frequently encountered was trichophyton rubrum (71.2%), fol ...201019886762
diagnostic pcr tests for microsporum audouinii, m. canis and trichophyton infections.since traditional diagnosis of dermatophyte infections is slow, we present a rapid new pcr test for detection of trichophyton spp., microsporum canis and m. audouinii infections. the performance of the test was evaluated with: 58 dermatophyte isolates; 10 yeast, mould and human dna control samples; 25 routine specimens from patients suspected of having dermatophytosis; 10 hair specimens from guinea pigs experimentally infected with m. canis; and two samples from un-infected control animals. dna ...201019886764
evaluation of a pcr melting profile method for intraspecies differentiation of trichophyton rubrum and trichophyton interdigitale.in order to identify the source of infections caused by dermatophytes, as well as the pathogen transmission pathway, there is a need to determine methods that allow detailed genetic differentiation of the strains within the dermatophyte genera. in this work, a pcr melting profile (pcr-mp) technique based on the ligation of adaptors and the difference in melting temperatures of dna restriction fragments was used for the first time for intraspecies genotyping of dermatophytes. clinical isolates an ...201019892858
organization and evolutionary trajectory of the mating type (mat) locus in dermatophyte and dimorphic fungal pathogens.sexual reproduction in fungi is governed by a specialized genomic region, the mating type (mat) locus, whose gene identity, organization, and complexity are diverse. we identified the mat locus of five dermatophyte fungal pathogens (microsporum gypseum, microsporum canis, trichophyton equinum, trichophyton rubrum, and trichophyton tonsurans) and a dimorphic fungus, paracoccidioides brasiliensis, and performed phylogenetic analyses. the identified mat locus idiomorphs of m. gypseum control cell t ...201019880755
antifungal activity of bis-azasqualenes, inhibitors of oxidosqualene cyclase.the antifungal activity and in vitro toxicity toward animal cells of two inhibitors of oxidosqualene cyclase, squalene bis-diethylamine (sbd) and squalene bis-diethylmethylammonium iodide (sbdi) were studied. minimum inhibitory concentration (mic) against dermatophytes and other fungi involved in cutaneous and systemic infections (12 isolates from seven species) were determined by the broth microdilution method based on the reference documents m38-a and m27-a2 of clinical and laboratory standard ...201019549106
dermatophyte species in superficial mycoses in the kraków district, poland in the years 1972-2007.considerable changes in the dermatophyte spectrum have been observed in the past century. hence, many authors point out the necessity of performing periodical overviews of the mycological flora producing mycoses in humans in a given area. analysis of dermatophyte species was performed, which were isolated from the lesions in patients suspected of superficial mycosis and referred to the department of mycology. the materials were isolated from patients suspected of superficial mycosis from kraków ...201019298357
tinea capitis among children in the columbus area, ohio, usa.tinea capitis is a fungal infection of the hair follicles of the scalp. in the us, the most common organisms have traditionally been trichophyton tonsurans, and occasionally microsporum canis. this study was designed to examine patterns of organisms causing tinea capitis and determine factors associated with infection. a retrospective database analysis was conducted to locate records of patients with tinea capitis from may 2001 to may 2006 at nationwide children's hospital in columbus, oh. descr ...201019302461
antimicrobial activity of hymenaea martiana towards dermatophytes and cryptococcus neoformans.the biological activity of crude extract and fractions of hymenaea martiana was evaluated against a panel of human pathogenic fungi. the crude extracts and hydroalcoholic fractions (e) showed a high activity against cryptococcus neoformans species complex isolates with mics between 2 and 64 μg ml(-1). the methanolic (c) and butanolic (d) fractions were the most active against trichopyton rubrum, trichopyton mentagrophytes and microsporum canis with mics between 8 and 256 μg ml(-1). none of the e ...201019563478
a 20-year survey of tinea faciei.tinea faciei is an uncommon dermatophytosis affecting the glabrous skin of the face. between 1988 and 2007 at the dermatology department of cagliari university, 107 cases of tinea faciei have been diagnosed, involving 72 females and 35 males, aged 2-72 years. incidence peaks were observed between 6 and 15 years (48.59%) and between 36 and 45 years (17.76%). males below and females above 15 years of age were the most affected. in 61 patients (57.1%), typical forms of tinea faciei were observed, w ...201019563490
recovery of dermatophytes in pet grooming tools from veterinary clinics and pet grooming salons.fomites such as brushes and clippers are known to be potential risk factors for the transmission of dermatophytes between pet animals. the aim of this study was to investigate this risk by examining those grooming implements for the presence of any viable dermatophytes.201020137008
cutaneous mycoflora and cd4:cd8 ratio of cats infected with feline immunodeficiency virus.this study was designed to compare cutaneous mycoflora isolation and cd4+:cd8+ ratio in feline immunodeficiency virus (fiv)-infected cats with that in fiv-uninfected cats. sixty cats were examined. twenty-five were fiv-infected cats and 35 were fiv-uninfected cats. all 60 cats were felv-negative. fungi were speciated and immunophenotyping of peripheral cd4+ and cd8+ t lymphocytes was performed. at least one fungal colony was isolated from 22/25 (88%) fiv-infected cats. among the fiv-uninfected c ...201020156698
evaluation of the association of superficial dermatophytosis and athletic activities with special reference to its prevention and control.this study was conducted to evaluate the association of superficial mycosis and athletic activities with special references to its prevention and control in tehran. participating in various kinds of sports can lead to direct and indirect exposures to and transmission of micro-organisms between athletes and also passive observers.201020636349
in vivo antifungal effect of combretum and terminalia species extracts on cutaneous wound healing in immunosuppressed rats.acetone leaf extracts of combretaceae species combretum imberbe wawra, combretum nelsonii duemmer, combretum albopunctatum suesseng, and terminalia sericea burch ex dc and a mixture of asiatic acid and arjunolic acid isolated from c. nelsonii were tested for antifungal activity against candida albicans, cryptococcus neoformans, microsporum canis, and sporothrix schenckii on wounds of immunocompromised wistar rats. the therapeutic agents were selected based on low mic values ranging 0.02-2.5 mg/m ...201020645734
microsporum canis antifungal susceptibilities: concerns regarding their clinical predictability. 201020685087
dermatophytes in republic of macedonia.(full text is available at http://www.manu.edu.mk/prilozi). a total of 1742 clinically suspected samples was examined for the incidence and distribution of dermatophytes in the republic of macedonia from june 2007 to jan. 2009. 600 dermatophytes were isolated and identified. in the studied period 9 different dermatophyte species were isolated, namely trichophyton rubrum (48.83%), microsporum canis (20%), trichophyton mentagrophytes var. interdigitale (16.83%), epidermophyton floccosum (4.17%), t ...201020693949
in vitro profiling of pramiconazole and in vivo evaluation in microsporum canis dermatitis and candida albicans vaginitis laboratory models.the triazole antifungal pramiconazole (stiefel, a gsk company) was compared with itraconazole, miconazole, and terbinafine in vitro and in vivo. potent in vitro activities against candida spp. (50% inhibitory concentration [ic₅₀], 0.04 to 1.83 μm) and microsporum and trichophyton spp. (ic₅₀, 0.15 to 1.34 μm) were obtained but not, however, against other filamentous molds and zygomycetes. in the m. canis guinea pig model and c. albicans vulvovaginitis rat model, pramiconazole was superior to the ...201020805398
efficacy of eight commercial formulations of lime sulphur on in vitro growth inhibition of microsporum canis.lime sulphur is a common topical treatment for dermatophytosis in animals. until recently, a single veterinary lime sulphur formulation was available. the purpose of this study was to compare the efficacy of eight lime sulphur products for in vitro growth inhibition of microsporum canis using the isolated infected spore model. infective m. canis spores were isolated from hairs collected from untreated cats. hairs were macerated in triton-x solution and isolated according to a previously publishe ...201020868396
geotrichum candidum as etiological agent of horse dermatomycosis.fungi of the genus geotrichum are commonly found in the environment and, in some circumstances, they may cause diseases in humans and animals. although these fungi have been isolated from skin lesions of some animal species, their pathogenic role in horses remains uncertain. with the aim to investigate the role of geotrichum candidum as etiological agent of dermatomycoses, a retrospective study of 64 horses presenting skin lesions and suspected to have fungal infections was carried out. for each ...201020970928
chemical composition and biological assays of essential oils of calamintha nepeta (l.) savi subsp. nepeta (lamiaceae).aerial parts of wild calamintha nepeta (l.) savi subsp. nepeta growing spontaneously on the mediterranean coast (sardinia island, italy) and on the atlantic coast (portugal) were used as a matrix for the supercritical extraction of volatile oil with co(2). the collected extracts were analysed by gc-fid and gc-ms methods and their compositions were compared with that of the essential oil isolated by hydrodistillation, but the differences were not relevant. a strong chemical variability was observ ...201020981614
composition of a volatile extract of eryngium duriaei subsp. juresianum (m. laínz) m. laínz, signalised by the antifungal activity.the composition of a volatile extract of eryngium duriaei subsp. juresianum, signalised by the antifungal activity (mic values=0.16-0.32 μl ml(-1)) against several dermatophyte species (trichophyton mentagrophytes, t. rubrum, epidermophyton floccosum; t. verrucosum, t. mentagrophytes var interdigitale, microsporum canis and m. gypseum) was established following a combined methodology of gc, gc-ms and an exclusive (13)c nmr technique that does not require prior isolation of compounds. twenty-five ...201021036502
effects of temperature variations and light exposure on the time to growth of dermatophytes using six different fungal culture media inoculated with laboratory strains and samples obtained from infected cats.in this study, 5/6 commercially available fungal culture media were comparable with respect to first growth, first colour change, and first sporulation when inoculated with three strains of microsporum canis, one strain of microsporum gypseum, and one strain of trichophyton species when incubated at either 25°c or 30°c. five of six plates showed 100% growth at both temperatures. five of six plates showed 100% growth when inoculated with naturally infective m canis hairs and spores. one commercia ...201021051255
isolation of dermatophytes from dogs and cats with suspected dermatophytosis in western turkey.the aim of this study was to determine the species of dermatophytes isolated from dogs and cats and their prevalence in the two big provinces of western turkey. a total of 362 animals (198 dogs and 164 cats) with skin lesions (alopecia and desquamation) were examined from march 2006 to february 2008. of the 362 samples examined, 52 (14.4%) were positive for fungal elements by direct microscopic examination, and 70 (19.3%) were culture positive for dermatophytes. the isolation rates of dermatophy ...201021126787
the spectrum of dermatological disorders among primary school children in dar es salaam.dermatologic disorders are common in many countries but the spectrum varies greatly. many studies have reported a significant burden of skin diseases in school children. the objective of this study was to determine the current spectrum of dermatological disorders in primary school children in dar es salaam city.201021162714
synthesis and antifungal activity of 1-aryl-3-phenethylamino-1-propanone hydrochlorides and 3-aroyl-4-aryl-1-phenethyl-4-piperidinols.mono-mannich bases, 1-aryl-3-phenethylamino-1-propanone hydrochlorides, 1a, 2a, 3a, 4a, 5a, 6a, 7a, 8a, 9a, and semi-cyclic mono-mannich bases, 3-aroyl-4-aryl-1-phenethyl-4-piperidinols, 1b, 2b, 3b, 4b, 5b, 6b, 7b, 8b, 9b, were synthesized by a non-classical mannich reaction. the aryl part was: c(6)h(5 )for 1a, 1b; 4-ch(3)c(6)h(4) for 2a, 2b; 4-ch(3)oc(6)h(4 )for 3a, 3b; 4-clc(6)h(4 )for 4a, 4b; 4-fc(6)h(4) for 5a, 5b; 4-brc(6)h(4) for 6a, 6b; 2,4-(cl)(2)c(6)h(3) for 7a, 7b; 4-no(2)c(6)h(4 )for ...201020232370
synthesis and antileishmanial and antimicrobial activities of some 2,3-disubstituted 3h-quinazolin-4-ones.a series of 2,3-disubstituted 3h-quinazolin-4-ones was synthesized. antimicrobial activities of the synthesized compounds were investigated against gram (+ve) and gram (-ve) bacteria, including b. subtilis, s. aureus, s. flexneri, p. aeruginosa, and s. typhi, and six fungi, namely trichophyton longifusus, candida albicans, aspergillus flavus, microsporum canis, fusarium solani, and candida glabrata using the broth microdilution method. compounds 9, 11, and 12 showed significant activities agains ...201020235747
epidemiology and risk factors for dermatophytoses in rabbit farms.while dermatophytoses of several animal species have been extensively investigated, information on their occurrence and epidemiology in rabbits is limited. we carried out a study from october 2006 to february 2007 of 23 rabbit farms in apulia and basilicata regions (southern italy) in order to investigate the occurrence and risk factors associated with dermatophytoses in breeding rabbits. dermatophytes were isolated from 86.9% (20/23) of the farms and from 51.8% (420/810) of the animals sampled. ...201020297949
[tinea capitis: two years experience in a paediatric hospital of buenos aires, argentina].tinea capitis is an infection caused by dermatophytes of the genera microsporum and trichophyton, and constitutes a major health problem in argentina. the aim of the present study was to find out the incidence of those etiological agents and the therapeutic response in patients attending a high-complexity paediatric hospital within a two-year period. a total of 98 tinea capitis were diagnosed, 13 of which were celsus kerion. microsporum canis was isolated in 61.28%. the range of values for minim ...201020346290
disease progression of dermatophytic pseudomycetoma in a persian cat.dermatophytic pseudomycetoma is a subcutaneous fungal infection by microsporum canis.201020346294
a zoonotic ringworm outbreak caused by a dysgonic strain of microsporum canis from stray cats.cats are frequent carriers of microsporum canis and veterinary students are at high risk of exposure and acquisition of the organism a la infección.201020346301
inflammatory tinea capitis: kerion, dermatophytic granuloma, and mycetoma.inflammatory tinea capitis is the result of a hypersensitivity reaction to a dermatophytic infection. the usual forms are favus, kerion celsi, dermatophytic majocchi granuloma, and mycetoma. inflammatory tinea capitis can be caused by microsporum canis, trichophyton mentagrophytes, t tonsurans, t rubrum, and m gypseum. histopathologic findings include a spectrum from mild suppurative folliculitis to dense granulomatous infiltrates. in mycetoma, grains must be present.201020347653
extraction, separation and isolation of volatiles from vitex agnus-castus l. (verbenaceae) wild species of sardinia, italy, by supercritical co2.isolation of volatile concentrates from leaves, flowers and fruits of vitex agnus-castus l. have been obtained by supercritical extraction with carbon dioxide. the composition of the volatile concentrates has been analysed by gc/ms. in all plant organs, the extracts are composed chiefly of alpha-pinene, sabinene, 1,8-cineole, alpha-terpinyl acetate, (e)-caryophyllene, (e)-beta-farnesene, bicyclogermacrene, spathulenol and manool. the main difference observed was in the content of sclarene, which ...201020397107
[dermatophytoses due to anthropophilic fungi in cadiz, spain, between 1997 and 2008].cutaneous fungal infections are a major public health problem. the distribution of the dermatophytoses varies between countries and geographical areas. the aim of this study was to determine the incidence, epidemiology, etiology, and clinical course of the dermatophytoses caused by anthropophilic fungi in cadiz, spain, over the past 12 years.201020398600
majocchi granuloma caused by microsporum canis as tinea incognito. 201020406391
favus of the scalp: an overview and update.tinea capitis favosa, a chronic inflammatory dermatophyte infection of the scalp, affects over 90% of patients with anthropophilic trichophyton schoenleinii. t. violaceum, t. verrucosum, zoophilic t. mentagrophytes (referred to as 'var. quinckeanum'), microsporum canis, and geophilic m. gypseum have also been recovered from favic lesions. favus is typically a childhood disease, yet adult cases are not uncommon. interestingly, favus is less contagious than other dermatophytoses, although intrafam ...201020411336
development of efficient tools for genetic manipulation of dermatophytes.molecular biological approaches have recently begun to be applied to molecular genetics studies of dermatophytes. high-throughput gene analysis methodologies, such as est sequencing, differential cdna screening, and cdna-based microarray analysis have been used to obtain information on many dermatophyte genes and their expression profiles under different experimental conditions. in addition, whole genome sequencing projects are underway for several important dermatophytes, such as trichophyton r ...201020467196
[emergence of microsporum audouinii and trichophyton tonsurans as causative organisms of tinea capitis in the dominican republic].tinea capitis affects mainly children. the frequency and causative organism vary from region to region.201020487688
fungalysin and dipeptidyl-peptidase gene transcription in microsporum canis strains isolated from symptomatic and asymptomatic cats.microsporum canis is the main pathogenic fungus that causes a superficial cutaneous infection called dermatophytosis in domestic carnivores. in cats, m. canis causes symptomatic or asymptomatic infection. recent conflicting data raise the question of whether the clinical status of the infected cat (symptomatic or asymptomatic) is directly correlated to the proteolytic activity of m. canis strains. here, the transcription of fungalysin and dipeptidyl-peptidase genes (dpp) of m. canis was compared ...201020488631
tinea capitis in adults in tunisia.objective: to determine the pattern of infectious agents causing tinea capitis (tc) in adult patients in the center of tunisia. methods: from january 1990 to december 2005, we have retrospectively collected all cases of adult tc, confirmed by the mycological examination. results: sixty patients (18 male, 42 female) with a mean age of 34.5 years were diagnosed as having adult tc among a total number of 1137 cases of tc (5.27%). clinical features were polymorphic and diagnosis was made on mycologi ...201020534084
majocchi's granuloma caused by microsporum canis in an immunocompetent patient. 201019832861
detection of antimicrobial compounds by bioautography of different extracts of leaves of selected south african tree species.the hexane, acetone, dichloromethane and methanol extracts of combretum vendae a.e. van wyk (combretaceae), commiphora harveyi (engl.) engl. (burseraceae), khaya anthotheca (welm.) c.dc (meliaceae), kirkia wilmsii engl. (kirkiaceae), loxostylis alata a. spreng. ex rchb. (anacardiaceae), ochna natalitia (meisn.) walp. (ochnaceae) and protorhus longifolia (bernh. ex c. krauss) engl. (anacardiaceae) were screened for their antimicrobial activity. the test organisms included bacteria (enterococcus f ...200921304615
chemical constituents from the leaves of boehmeria rugulosa with antidiabetic and antimicrobial activities.three new flavonoid glycosides, named chalcone-6'-hydroxy-2',3,4-trimethoxy-4'-o-beta-d-glucopyranoside (1), isoflavone-3',4',5,6-tetrahydroxy-7-o-[beta-d-glucopyranosyl-(1-->3)-alpha-l-rhamnopyranoside] (2), and isoflavone-3',4',5,6-tetrahydroxy-7-o-[beta-d-glucopyranosyl-(1-->6)-beta-d-glucopyranosyl-(1-->6)-beta-d-glucopyranosyl-(1-->3)-alpha-l-rhamnopyranoside] (3), were isolated from the leaves of boehmeria rugulosa, together with five known compounds, beta-sitosterol, quercetin, 3,4-dimeth ...200920183275
in vitro activity of tachyplesin iii alone and in combination with terbinafine against clinical isolates of dermatophytes.aim of our study was to investigate the in vitro effects of tachyplesin iii (tp), a potent disulfide-linked peptide, in dermatophytes infections, with respect to or in combination with terbinafine (terb), against 20 clinical isolates of dermatophytes belonging to four species. a broth microdilution method following the clsi recommendations (m38-a) was used for testing drugs alone and in combination. terb mics were significantly lower than those observed for tp (p<0.001). testing for antifungal a ...200919591887
development of transformation system for trichophyton rubrum by electroporation of germinated conidia.dermatophytes are the fungi that can cause infections of skin, hair, and nails due to their ability to utilize keratin. the genetic transformation systems of dermatophytes were successfully applied to trichophyton mentagrophytes and microsporum canis. here we describe the procedure for genetic transformation of trichophyton rubrum by electroporation of their germinated conidia. a linearized transformation vector (pchsh75-pch/gfp/ttrpc) containing bacterial hygromycin b phosphotransferase gene (h ...200919629488
[microsporum canis on dermatologically healthy cats in temuco city, chile].microsporum canis is the most common cause of feline dermatophytosis and the most pathogenic fungus isolated from the skin and hair of healthy cats. cats are considered to be the natural reservoir and infection sourse of this disease in human and domestic animals.200919635446
antioxidant and antidermatophytic activities of essential oil and extracts of magnolia liliflora desr.this study was carried out to assess the antioxidant and antidermatophytic activities of the essential oil and extracts of magnolia liliflora desr. antioxidant activity was evaluated by using 1,1-diphenyl-2-picrylhydrazyl (dpph) assay. the free radical scavenging activities of the oil and ethyl acetate extract were found to be superior (ic(50) values=10.11 and 16.17 microg/ml, respectively) as compared to butylatedhydreoxyanisole (bha), (ic(50) value=18.27 microg/ml). also the ethyl acetate extr ...200919635519
antioxidant and antidermatophytic activities of essential oil and extracts of metasequoia glyptostroboides miki ex hu.this study was undertaken to assess the antioxidant and antidermatophytic potential of the essential oil and extracts (hexane, chloroform, ethyl acetate and methanol) of metasequoia glyptostroboides miki ex hu. antioxidant activity was evaluated by using 1,1-diphenyl-2-picrylhydrazyl (dpph) assay. the free radical scavenging activities of the oil and ethyl acetate extract were found to be superior (ic(50)=9.1 and 14.24 microg/ml, respectively) as compared to butylatedhydroxyanisole (bha), (ic(50 ...200919303043
antimicrobial dihydroisocoumarins from crassocephalum biafrae.bioassay-guided fractionation of the chcl (3)-soluble extract of the stem bark of crassocephalum biafrae (asteraceae) resulted in the isolation of three new dihydroisocoumarins, named biafraecoumarins a ( 1), b ( 2), and c ( 3); two known triterpenes ( 4 and 5); and a known ceramide ( 6). the structures of the new compounds were established as 7-but-15-enyl-6,8-dihydroxy-3( r)-penta-9,11-dienylisochroman-1-one ( 1), 7-butyl-6,8-dihydroxy-3( r)-penta-9,11-dienylisochroman-1-one ( 2), and 7-butyl- ...200919350487
heat resistance of dermatophyte's conidiospores from athletes kits stored in nigerian university sport's center.the incidence and heat resistance of conidiospores produced by dermatophytes isolated from athlete's kits (canvasses, stockings and spike shoes) stored in nigerian university sport's centre were investigated. epidermophyton floccosum, microsporum oudouinii, microsporum canis, trichophyton concentricum, trichophyton mentagrophytes and trichophyton rubrum were isolated and their incidence on the athlete's kits varied with the species and type of kits. among the isolates t. mentagrophytes, t. rubru ...200919388558
canine nodular dermatophytosis (kerion): 23 cases.dermatophytosis is a common zoonotic disease, and one of its clinical presentations in the dog is nodular dermatophytosis (kerion). because the infection is located within the dermis, routine diagnostic tests such as a wood's lamp examination, microscopic examination of hair shafts for fungal elements and fungal culture can yield negative results. in such cases, histopathological examination with routine and special stains (periodic acid-schiff, gomori methenamine silver) is required to confirm ...200919392766
antifungal potential of essential oil and various organic extracts of nandina domestica thunb. against skin infectious fungal pathogens.this study was undertaken to assess the in vitro antifungal potential of the essential oil and n-hexane, chloroform, ethyl acetate, and methanol extracts of nandina domestica thunb. against dermatophytes, the casual agents of superficial infections in animals and human beings. the oil (1,000 microg/disc) and extracts (1,500 microg/disc) revealed 31.1-68.6% and 19.2-55.1% antidermatophytic effect against trichophyton rubrum kctc 6345, t. rubrum kctc 6375, t. rubrum kctc 6352, trichophyton mentagr ...200919415265
tinea capitis in early infancy treated with itraconazole: a pilot study.tinea capitis is the most common fungal infection of the scalp in childhood, but a very rare disorder in the first year of life.200919453785
leptoglycin: a new glycine/leucine-rich antimicrobial peptide isolated from the skin secretion of the south american frog leptodactylus pentadactylus (leptodactylidae).antimicrobial peptides are components of innate immunity that is the first-line defense against invading pathogens for a wide range of organisms. here, we describe the isolation, biological characterization and amino acid sequencing of a novel neutral glycine/leucine-rich antimicrobial peptide from skin secretion of leptodactylus pentadactylus named leptoglycin. the amino acid sequence of the peptide purified by rp-hplc (c(18) column) was deduced by mass spectrometric de novo sequencing and conf ...200919298834
in vitro susceptibility of dermatophytes to conventional and alternative antifungal agents.the minimum inhibitory concentrations (mics), the minimal fungicidal concentrations (mfcs), the fungal biomass (fb) and hyphal viability employing the dye 3-4,5 dimethyl- 2-thiazolyl- 2,5- diphenyl- 2h tetrazolium bromide (mtt) were used to compare the in vitro effects of fluconazole (flu) with those of the n-terminal palmitoyl-lipidated peptide, pal-lys-lys-nh(2) (pal), and a tea tree oil component, gamma-terpinene (ter), against several clinical isolates of microsporum canis and trichophyton r ...200919115137
epidemiology of dermatophytoses in sfax, tunisia.the distribution of dermatophytes varies in different countries and geographical areas depending on several factors. to determine the frequency of aetiological agents and the clinical variants of dermatophytoses, we carried out a study between 1998 and 2007. out of 25 432 subjects suspected to have superficial mycoses, 9960 (39.2%) were affected with dermatophytoses; 14957 positive samples were obtained. the mean age was 35.7 years (range: 21 days to 97 years). sex ratio was 0.9. our patients we ...200919207834
in vitro pharmacodynamic characteristics of griseofulvin against dermatophyte isolates of trichophyton tonsurans from tinea capitis patients.tinea capitis is the most commonly observed fungal infection in childhood and is primarily caused by the dermatophyte species trichophyton tonsurans, microsporum canis, and trichophyton violaceum. in north america and the united kingdom t. tonsurans is responsible for more than 90% of cases. griseofulvin has been the treatment of choice for tinea capitis for more than 40 years and is the sole oral antifungal agent approved by the fda for the management of tinea capitis. some researchers have exp ...200919212895
in vitro susceptibility testing of dermatophytes isolated in goiania, brazil, against five antifungal agents by broth microdilution method.the antifungal activities of fluconazole, itraconazole, ketoconazole, terbinafine and griseofulvin were tested by broth microdilution technique, against 60 dermatophytes isolated from nail or skin specimens from goiania city patients, brazil. in this study, the microtiter plates were incubated at 28 masculinec allowing a reading of the minimal inhibitory concentration (mic) after four days of incubation for trichophyton mentagrophytes and five days for t. rubrum and microsporum canis. most of th ...200919229384
competence of the housefly, musca domestica, as a vector of microsporum canis under experimental conditions.the role of musca domestica linnaeus as a vector of the dermatophyte microsporum canis was investigated under experimental laboratory conditions. about 400 4-day-old m. domestica flies were divided into two groups. group a consisted of about 200 infected flies and group b comprised about 200 uninfected flies that were used as controls. each trial was run three times. flies from group a were fed for 24 h with a solution of ultra-high temperature-treated (uht) milk containing about 10(6) colony-fo ...200919239611
changing face of tinea capitis in europe.the aim of this review is to update the latest epidemiological situation in europe, explore recent issues in recognition and emerging opportunities in diagnosis and look at progressions in treatment. papers reviewed have, in the main, been published within the last 2 years.200919262376
antifungal and marker effects of talisia esculenta lectin on microsporum canis in vitro.the purpose of this study was to demonstrate the usefulness of lectin obtained from talisia esculenta (tel) seeds as a tool to recognize and study microsporum canis. for this purpose, we investigated the antifungal and marker action of this lectin and the relationship of these effects with the presence of carbohydrates on the structure of this fungus.200919558469
[summarization of tinea capitis cases encountered at a clinic in the past 5 years].twelve cases of tinea capitis were encountered at a clinic in kumamoto prefecture between april 2004 and december 2008. patients were boys aged between 2 and 18 in 11 cases, and a woman aged 50 years in 1 case. the pathogen was microsporum canis in 6 cases, and trichophyton tonsurans in 6 cases. in the cases with m. canis , patients were boys aged 2 to 8 years in 5 cases, and the type of disease was non-inflammatory in 5 cases and inflammatory in 1 case. the patients with t. tonsurans were boys ...200919654449
comparison of in vitro activity of five antifungal agents against dermatophytes, using the agar dilution and broth microdilution methods.the purpose of this study was to compare the agar dilution and broth microdilution methods for determining the minimum inhibitory concentration (mic) of fluconazole, itraconazole, ketoconazole, griseofulvin and terbinafine for 60 dermatophyte samples belonging to the species trichophyton rubrum, trichophyton mentagrophytes and microsporum canis. the percentage agreement between the two methods, for all the isolates with < 2 dilutions that were tested was 91.6% for ketoconazole and griseofulvin, ...200919684970
antifungal activity of eugenia umbelliflora against dermatophytes.antifungal activities of eugenia umbelliflora berg. (myrtaceae) were tested in vitro against a panel of standard and clinical isolates of human fungal pathogens (dermatophytes and opportunistic saprobes). methanol extracts of leaves and fruits of e. umbelliflora were separately prepared and partitioned, to yield dichloromethane (dcm), ethyl acetate (etoac) and aqueous fractions (aq). three compounds (1-3) were obtained from the dcm extract using chromatographic procedures. antifungal assays were ...200919831024
confirmed case of feline mycetoma due to microsporum canis.the first case of feline true mycetoma because of a dermatophyte has been reported in this manuscript, although pseudomycetoma has been described in dogs and cats. the patient was a 9-year-old castrated male persian cat weighing 4.2 kg with subcutaneous nodules on the dorsal trunk. physical examination revealed two subcutaneous nodules (2.5 x 5.0 cm and 3.5 x 5.0 cm in size) that drained purulent exudates with cement-like substances containing yellowish granules. the impression smear of the yell ...200918444972
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