Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| captures of western corn rootworm (coleoptera: chrysomelidae) adults with pherocon am and vial traps in four crops in east central illinois. | it is hypothesized that the long-term rotation of maize (zea mays l.) and soybean (glycine max l.) in east central illinois has caused a significant change in the ovipositional behavior of the western corn rootworm, diabrotica virgifera virgifera leconte. since the mid 1990s in east central illinois, western corn rootworm adults have been observed feeding on soybean foliage and also now use soybean fields as egg laying sites. this behavioral adaptation has greatly decreased the effectiveness of ... | 2003 | 12852611 |
| multiplex polymerase chain reaction method for differentiating western and northern corn rootworm larvae (coleoptera: chrysomelidae). | western corn rootworm, diabrotica virgifera virgifera leconte, and northern corn rootworm, d. barberi smith and lawrence, are sympatric species and serious pests of corn cultivation in north america. comparison of nucleotide sequence of mitochondrial cytochrome oxidase i and ii was used to design polymerase chain reaction (pcr) primers that discriminate immature stages of the two species based on differences in amplicon size. multiplex pcr can be used to give a positive test for each species in ... | 2003 | 12852603 |
| inheritance of methyl-parathion resistance in nebraska western corn rootworm populations (coleoptera: chrysomelidae). | field populations of western corn rootworm, diabrotica virgifera virgifera leconte, were collected from three different sites (york co., phelps co., and saunders co.) in nebraska during 1996. adult bioassays of these three populations were conducted with different concentrations of methyl-parathion and at a diagnostic concentration (1.0 microg/ml) to determine resistance levels among these populations. self and reciprocal crosses were made between the two resistant and one susceptible laboratory ... | 2003 | 12650355 |
| impact of cysteine proteinase inhibition in midgut fluid and oral secretion on fecundity and pollen consumption of western corn rootworm (diabrotica virgifera virgifera). | cysteine proteinases predominate in the midgut fluid (mf) and oral secretion (os) of adult western corn rootworm (wcr) based on their mild acidic ph optima (ph 6.0), enhanced activities after treatment with thiol reducing agents, and inhibition by selective cysteine proteinase inhibitors (pis). four cysteine pis including e-64, calpeptin, calpain inhibitor ii, and leupeptin (also a serine pi) strongly inhibited azocaseinolytic activity in a dose-dependent manner in both the mf and os. the most s ... | 2003 | 12587142 |
| entomopathogenic nematodes in the european biocontrol market. | in europe total revenues in the biocontrol market have reached approximately 200 million euros. the sector with the highest turn-over is the market for beneficial invertebrates with a 55% share, followed by microbial agents with approximately 25%. annual growth rates of up to 20% have been estimated. besides microbial plant protection products that are currently in the process of re-registration, several microbial products have been registered or are in the process of registration, following the ... | 2003 | 15149088 |
| economics versus alleles: balancing integrated pest management and insect resistance management for rotation-resistant western corn rootworm (coleoptera: chrysomelidae). | western corn rootworm, diabrotica virgifera virgifera leconte, has overcome crop rotation in several areas of the central united states. we expanded a simple model of adult behavior and population genetics to explain how rotation resistance may have developed and to study ways to manage the western corn rootworm in a landscape of corn, soybean, and winter wheat where evolution of resistance may occur. we modeled six alternative management strategies over a 15-yr time horizon, as well as a strate ... | 2003 | 14977129 |
| transect sampling to enhance efficiency of corn rootworm (coleoptera: chrysomelidae) monitoring in corn. | crop monitoring for adult corn rootworms, diabrotica virgifera virgifera leconte and diabrotica barberi smith and lawrence, remains the best means to assess fields at risk from this pest if replanted to corn, zea mays (l.). improvements in sampling methodology, including the development of a sequential sampling plan, have reduced the minimum sampling time required to make a management decision to 20 min or less per field per visit. however, many growers and crop consultants still find this time ... | 2003 | 14650514 |
| antifeedant effects of proteinase inhibitors on feeding behaviors of adult western corn rootworm (diabrotica virgifera virgifera). | low-molecular-weight peptidyl proteinase inhibitors (pis) including leupeptin, calpain inhibitor i, and calpeptin were found to be potent antifeedants for adult western corn rootworm (wcr) against the phagostimulation of cucurbitacin b (cuc b) or a corn pollen extract (cpe). leupeptin was the strongest (ed50 = 0.36 and 0.55 nmol/disk for cuc b and cpe, respectively) among pis tested with an antifeedant potency much stronger than the steroid progesterone (ed50 = 2.29 and 5.05 nmol/disk for cuc b ... | 2003 | 12775144 |
| post-establishment movement of western corn rootworm larvae (coleoptera: chrysomelidae) in central missouri corn. | if registered, transgenic corn, zea mays l., with corn rootworm resistance will offer a viable alternative to insecticides for managing diabrotica spp. corn rootworms. resistance management to maintain susceptibility is in the interest of growers, the environmental protection agency, and industry, but little is known about many aspects of corn rootworm biology required for an effective resistance management program. the extent of larval movement by the western corn rootworm, diabrotica virgifera ... | 2003 | 12852594 |
| a monitoring trap for diabrotica virgifera virgifera and d. barberi adults lured with a poisoned cucurbitacin bait. | a trap is described that uses as a simple natural ingredient a cucurbitacin mixture to capture and carbaryl insecticide to kill northern and western corn rootworm beetles (diabrotica barberi smith and lawrence, and diabrotica virgifera virgifera leconte (d.v.v.), respectively). the trap is consistent in numbers of beetles captured per trap per day, and it should be useful in integrated pest management programs to monitor the population density of rootworm beetles in corn fields. captures between ... | 2003 | 15149093 |
| effects of different growing systems and fertiliser rates on attractiveness of maize crop to beetles of diabrotica virgifera virgifera leconte and larvae dammage. | monitoring of plant lodging, yield reaction due to root injures caused by western corn rootworm larvae (wcr) (diabroticia virgifera virgifera le conte) and adults abundance with yellow multigard and pherocone am and pheromone csalomon traps, were performed in three field trials. first one with 4 variants of maize growing system; maize continuous cropping, two crop rotation (wheat-maize, soybean-maize) and three crop rotation (wheat-soybean-maize), set up in 1985. second one with 54 variants, bot ... | 2003 | 15149092 |
| characterization of general esterases from methyl parathion-resistant and -susceptible populations of western corn rootworm (coleoptera: chrysomelidae). | a consistent correlation between elevated esterase activity and methyl parathion resistance among nebraska western corn rootworm, diabrotica virgifera virgifera leconte, populations has previously been documented. characterization of general esterase activity using naphtholic esters as model substrates indicated that differences between resistant and susceptible strains could be maximized by optimizing assay conditions. the optimal conditions identified here were similar to those reported for ot ... | 2003 | 14977127 |
| in-field labeling of western corn rootworm adults (coleoptera: chrysomelidae) with rubidium. | field and laboratory studies were conducted in 2000 and 2001 to determine the feasibility of mass marking western corn rootworm adults, diabrotica virgifera virgifera leconte, with rbcl in the field. results showed that application of rubidium (rb) in solution to both the soil (1 g rb/plant) and whorl (1 g rb/plant) of corn plants was optimal for labeling western corn rootworm adults during larval development. development of larvae on rb-enriched corn with this technique did not significantly in ... | 2003 | 14977112 |
| basic biology and small-scale rearing of celatoria compressa(diptera: tachinidae), a parasitoid of diabrotica virgifera virgifera (coleoptera: chrysomelidae). | the tachinid celatoria compressa wulp has been evaluated as a candidate biological control agent for the western corn rootworm, diabrotica virgifera virgiferaleconte, in europe, where it is an invasive alien pest of maize. special emphasis has been placed on understanding aspects of the parasitoid basic biology and on developing a rearing technique for a small-scale production of c. compressapuparia. the age of c. compressa adults was found to be the most crucial factor in achieving mating. only ... | 2003 | 14704104 |
| a spatially explicit model simulating western corn rootworm (coleoptera: chrysomelidae) adaptation to insect-resistant maize. | a stochastic spatially explicit computer model is described that simulates the adaptation by western corn rootworm, diabrotica virgifera virgifera leconte, to rootworm-resistance traits in maize. the model reflects the ecology of the rootworm in much of the corn belt of the united states. it includes functions for crop development, egg and larval mortality, adult emergence, mating, egg laying, mortality and dispersal, and alternative methods of rootworm control, to simulate the population dynami ... | 2003 | 14650529 |
| effects of row spacing and plant density on corn rootworm (coleoptera: chrysomelidae) emergence and damage potential to corn. | planting corn, zea mays l., in row spacings less than the conventional width of 76 cm has been shown to increase grain yields. this study was conducted to determine if row spacing and plant density affected corn rootworm, diabrotica virgifera virgifera leconte and d. barberi smith & lawrence, adult emergence, larval injury to the roots, and plant tolerance to injury. field experiments were conducted at ames and nashua, ia, in 1998, 1999, and 2000. treatments were row spacings of 38 and 76 cm, an ... | 2002 | 12076002 |
| diagnostic assays based on esterase-mediated resistance mechanisms in western corn rootworms (coleoptera: chrysomelidae). | resistance to methyl-parathion among nebraska western corn rootworm, diabrotica virgifera virgifera leconte, populations is associated with increased hydrolytic metabolism of an organophosphate insecticide substrate. an electrophoretic method to identify resistant individuals based on the staining intensity of esterase isozymes on nondenaturing polyacrylamide gels was developed. three groups of esterases (i, ii, and iii) were visible on the gels, but only group ii esterase isozymes were intensif ... | 2002 | 12539840 |
| comparison of adult corn rootworm (coleoptera: chrysomelidae) sampling methods. | studies were conducted in kansas corn and soybean fields during 1997 to compare various sampling methods, traps, and trap components for capturing three species of adult corn rootworms: western (diabrotica virgifera virgifera leconte), southern (d. undecimpunctata howardi barber), and northern (d. barberi smith & lawrence). lure constituents affected the species of beetle attracted to the trap. traps with a lure containing 4-methoxycinnamaldehyde attracted more western corn rootworms, those with ... | 2002 | 11942770 |
| distribution of mca-coated grits in maize fields after high wheel tractor application for disrupting orientation of diabrotica virgifera virgifera leconte. | high wheel tractor applications of 4-methoxycinnamaldehyde (mca)-coated corn granules ('grits') were conducted in ruski krstur (serbia) in summer 2001 in a 5 ha corn field. grits are a by-product after corn is harvested and separated from the cob and used as a carrier medium to disseminate mca into the corn field. mca is a kairomone mimic derived form cucurbita maxima (duchesne) used to disrupt orientation of diabrotica virgifera virgifera leconte towards different mca and pheromone baited traps ... | 2002 | 12696417 |
| effects of a potato cysteine proteinase inhibitor on midgut proteolytic enzyme activity and growth of the southern corn rootworm, diabrotica undecimpunctata howardi (coleoptera: chrysomelidae). | the major proteinase activity in extracts of larval midguts from the southern corn rootworm (scr), diabrotica undecimpunctata howardi, was identified as a cysteine proteinase that prefers substrates containing an arginine residue in the p1 position. gelatin-zymogram analysis of the midgut proteinases indicated that the artificial diet-fed scr, corn root-fed scr, and root-fed western corn rootworms (diabrotica virgifera virgifera) possess a single major proteinase with an apparent molecular mass ... | 2002 | 11886775 |
| binary insecticidal crystal protein from bacillus thuringiensis, strain ps149b1: effects of individual protein components and mixtures in laboratory bioassays. | a family of novel binary insecticidal crystal proteins, with activity against western corn rootworm, diabrotica virgifera virgifera leconte, was identified from bacillus thuringiensis berliner. a binary insecticidal crystal protein (bicp) from b. thuringiensis strain ps149b1 is composed of a 14-kda protein (cry34abl) and a 44-kdaprotein (cry35ab1). these proteins have been co-expressed in transgenic maize plants, zea mays l., and effectively control western corn rootworm larvae under field condi ... | 2002 | 12076012 |
| novel bacillus thuringiensis binary insecticidal crystal proteins active on western corn rootworm, diabrotica virgifera virgifera leconte. | a new family of insecticidal crystal proteins was discovered by screening sporulated bacillus thuringiensis cultures for oral activity against western corn rootworm (wcr) larvae. b. thuringiensis isolates ps80jj1, ps149b1, and ps167h2 have wcr insecticidal activity attributable to parasporal inclusion bodies containing proteins with molecular masses of ca. 14 and 44 kda. the genes encoding these polypeptides reside in apparent operons, and the 14-kda protein open reading frame (orf) precedes the ... | 2002 | 11872461 |
| insecticidal proteins from bacillus thuringiensis protect corn from corn rootworms. | field tests of corn co-expressing two new delta-endotoxins from bacillus thuringiensis (bt) have demonstrated protection from root damage by western corn rootworm (diabrotica virgifera virgifera leconte). the level of protection exceeds that provided by chemical insecticides. in the bacterium, these proteins form crystals during the sporulation phase of the growth cycle, are encoded by a single operon, and have molecular masses of 14 kda and 44 kda. corn rootworm larvae fed on corn roots express ... | 2001 | 11433280 |
| field resistance of two soybean germplasm lines, hc95-15mb and hc95-24mb, against bean leaf beetle (coleoptera: chrysomelidae), western corn rootworm (coleoptera: chrysomelidae), and japanese beetles (coleoptera: scarabaidae). | two recently released, mexican bean beetle, epilachna varivestis, mulsant, resistant soybean, glycine max (l.) merrill, germplasm lines, hc95-15mb and hc95-24mb, were examined for foliar and pod feeding resistance to adult bean leaf beetles, cerotoma trifurcata (forster), western corn rootworms, diabrotica virgifera virgifera leconte, and japanese beetles, popillia japonica newman. both lines were planted along with a susceptible control cultivar in 18 by 30-m plots and separate 0.8-ha size fiel ... | 2001 | 11777070 |
| linkages between fao agroclimatic data resources and the development of gis models for control of vector-borne diseases. | the food and agriculture organization (fao) of the united nations is the largest specialized un agency dealing with agriculture, forestry and fishery, particularly in the developing countries. one of its technical services, placed under the sustainable development department, has the responsibility to provide information on environment and natural resources as related to food and agriculture. it includes, among others, expertise in remote sensing, geographic information systems and agrometeorolo ... | 2001 | 11378139 |
| predicting western corn rootworm (coleoptera: chrysomelidae) larval injury to rotated corn with pherocon am traps in soybeans. | crop rotation for portions of east central illinois and northern indiana no longer adequately protects corn (zea mays l.) roots from western corn rootworm, diabrotica virgifera virgifera leconte. seventeen growers in east central illinois monitored western corn rootworm adults in soybean (glycine max l.) fields with unbaited pherocon am traps during 1996 and 1997. in the following years (1997 and 1998), growers left untreated strips (no insecticide applied) when these fields were planted with co ... | 2001 | 11233140 |
| influence of western corn rootworm (coleoptera: chrysomelidae) larval injury on yield of different types of maize. | two field experiments were conducted in 1995-1996 to determine if there are common yield responses among maize hybrids to larval western corn rootworm, diabrotica virgifera virgifera leconte injury. three yellow dent hybrids, five white food grade dent hybrids, and a popcorn hybrid were included in the study. the minimum level of rootworm injury as measured by root damage ratings (3.2-4.2) that significantly reduced yield was similar across the hybrids included in the study. however, the pattern ... | 2001 | 11233098 |
| modeling the dynamics of adaptation to transgenic corn by western corn rootworm (coleoptera: chrysomelidae). | a simulation model of the population dynamics and genetics of the western corn rootworm, diabrotica virgifera virgifera leconte, was created for a landscape of corn, soybean, and other crops. although the model was created to study a 2-locus problem for beetles having genes for resistance to both crop rotation and transgenic corn, during this first phase of the project, the model was simulated to evaluate only resistance management plans for transgenic corn. allele expression in the rootworm and ... | 2001 | 11332850 |
| distribution patterns of mca-coated corn grit formulation after aerial application to maize fields. | field studies in corn (zea mays l.) were conducted during the summer of 2000 to evaluate distribution patterns of corn grits treated with mca (4-methoxy cinnamaldehyde). aerial application was used in a 8 ha corn field situated in south-east hungary. corn granules ('grits') of 10-12 mesh size were applied on july 18th, august 1st and august 28th at rates of 18.3, 21.9 and 20.2 kg grits/ha and were covered with 80, 119 and 120 g mca/ha. experiments were evaluated by collecting grits in 30-cm plas ... | 2001 | 12425055 |
| consumption of residue containing cucurbitacin feeding stimulant and reduced rates of carbaryl insecticide by western corn rootworm (coleoptera: chrysomelidae). | application of insecticide at a reduced rate with a cucurbitacin-based feeding stimulant is a viable alternative to a broadcast insecticide application for control of adult western corn rootworms, diabrotica virgifera virgifera, leconte. because of the small amount of material applied, it is conceivable that a high density of beetles could consume all of the spray residue before economic control is achieved. a laboratory experiment was conducted to determine the amount of cucurbitacin-based spra ... | 2001 | 11777045 |
| evidence of evolving carbaryl resistance in western corn rootworm (coleoptera: chrysomelidae) in areawide-managed cornfields in north central kansas. | susceptibility of adult populations of the western corn rootworm, diabrotica virgifera virgifera leconte, to carbaryl was determined by a survey in 1996 before the implementation of an areawide management program near scandia in north central kansas. subsequently, the susceptibility of western corn rootworm adults to carbaryl has been monitored throughout the program from 1997 to 2000 in both control and managed areas. in 1996, adults were highly susceptible to carbaryl with a mean lc50 value of ... | 2001 | 11561854 |
| single and blended maize volatiles as attractants for diabroticite corn rootworm beetles. | synthetic maize volatiles and analogs dispensed singly and blended were tested for attractiveness to western (wcr, diabrotica virgifera virgifera) and northern corn rootworm beetles (ncr, d. barberi) in maize fields. newly identified attractants included syn-benzaldoxime, especially for ncr, and beta-caryophyllene for wcr females. (+/-)-linalool was more effective than was (-)-linalool. myrcene, (+)-beta-pinene, and (-)-beta-pinene were unattractive. adding methyl salicylate to (+/-)-linalool, ( ... | 2001 | 11504034 |
| expression and induction of three family 4 cytochrome p450 (cyp4)* genes identified from insecticide-resistant and susceptible western corn rootworms, diabrotica virgifera virgifera. | we have previously determined that cytochrome p450-based oxidation is involved in resistance to the insecticides methyl parathion and carbaryl in geographically distinct nebraska western corn rootworm populations. three new family 4 cytochrome p450 (cyp4) gene fragments (cyp4aj1, cyp4g18 and cyp4ak1) were cloned and sequenced from insecticide-resistant and -susceptible western corn rootworms. insecticide bioassays indicated the resistant population employed in this study was significantly resist ... | 2001 | 11422509 |
| larval susceptibility of an insecticide-resistant western corn rootworm (coleoptera: chrysomelidae) population to soil insecticides: laboratory bioassays, assays of detoxification enzymes, and field performance. | soil insecticides were evaluated in laboratory and field studies against larvae of an insecticide resistant population (phelps county, ne) of western corn rootworm, diabrotica virgifera virgifera leconte. insecticide toxicity was evaluated by topical application of technical insecticides to 3rd instars from saunders county, ne (susceptible) and phelps county populations. resistance ratios (ld50 phelps county/ld50 saunders county) for the insecticides methyl parathion, tefluthrin, carbofuran, ter ... | 2000 | 14658504 |
| identification and quantification of hydroxamic acids in maize seedling root tissue and impact on western corn rootworm (coleoptera: chrysomelidae) larval development. | hydroxamic acid content was analyzed in the root tissue of four maize, zea mays l., lines using high-performance liquid chromatography (hplc) and related to western corn rootworm, diabrotica virgifera virgifera leconte, larval development and survivorship. maize lines evaluated included mp710 (pi 596627), mpswcb-4, (pi 550498), sc213 (pi 548792), and dk580 (dekalb commercial hybrid). maize plants from each line were grown in test tubes containing a transparent agarose gel medium in a growth cham ... | 2000 | 10902360 |
| impact of western corn rootworm (coleoptera: chrysomelidae) on sweet corn and evaluation of insecticidal and cultural control options. | the western corn rootworm, diabrotica virgifera virgifera leconte, is an important pest of corn, zea mays l., causing yield losses from root damage, plant lodging, and silk feeding. because little is known about its impact on sweet corn, we conducted research to evaluate the combined effects of insecticide, planting date, and cultivar on root damage, plant lodging, and yield in central new york sweet corn. we also examined the influence of planting date and cultivar on the emergence of adult wes ... | 2000 | 10902334 |
| cdna cloning, biochemical characterization and inhibition by plant inhibitors of the alpha-amylases of the western corn rootworm, diabrotica virgifera virgifera. | we report the characterization and cdna cloning of two alpha-amylase isozymes from larvae of the western corn rootworm (diabrotica virgifera virgifera leconte). larvae raised on artificial media have very low levels of amylase activity, and much higher levels are found in larvae raised on maize seedlings. at ph 5.7, the optimum ph for enzyme activity, the alpha-amylases are substantially but not completely inhibited by amylase inhibitors from the common bean (phaseolus vulgaris) and from wheat ( ... | 2000 | 10899464 |
| a plant defensive cystatin (soyacystatin) targets cathepsin l-like digestive cysteine proteinases (dvcals) in the larval midgut of western corn rootworm (diabrotica virgifera virgifera). | feeding bioassay results established that the soybean cysteine proteinase inhibitor n (soyacystatin n, scn) substantially inhibits growth and development of western corn rootworm (wcr), by attenuating digestive proteolysis [zhao, y. et al. (1996) plant physiol. 111, 1299-1306]. recombinant scn was more inhibitory than the potent and broad specificity cysteine proteinase inhibitor e-64. wcr digestive proteolytic activity was separated by mildly denaturing sds-page into two fractions and in-gel as ... | 2000 | 10760514 |
| an in-gel assay of a recombinant western corn rootworm (diabrotica virgifera virgifera) cysteine proteinase expressed in yeast. | 2000 | 10860514 | |
| evaluation of conventional resistance to european corn borer (lepidoptera: crambidae) and western corn rootworm (coleoptera: chrysomelidae) in experimental maize lines developed from a backcross breeding program. | plant resistance is a promising control method for the two most damaging insect pests of maize, zea mays l.: the european corn borer, ostrinia nubilalis (hübner), and the western corn rootworm diabrotica virgifera virgifera leconte. fifteen experimental lines of maize, derived from a backcross breeding program designed to introgress resistance to european corn borer from peruvian maize into two u.s. corn belt adapted inbred lines, were evaluated for resistance to european corn borer and western ... | 2000 | 11142317 |
| foraging resources of adult mexican corn rootworm (coleoptera: chrysomelidae) in bell county, texas. | pollen analyses were used to determine pollen foraging resources of adult mexican corn rootworms, diabrotica virgifera zeae krysan & smith, captured near temple, bell county, tx, in 1996 and 1997. in 1996, adult mexican corn rootworms were captured in a corn, zea mays l., field. in 1997, nine locations outside of cornfields were added. overall, 92% of the beetles (n = 1,323) contained pollen. more than 142,000 pollen grains were counted, representing 45 families, 63 genera, and 27 species. overa ... | 2000 | 10902309 |
| genetic variation in geographical populations of western and mexican corn rootworm. | genetic variation in the nuclear rdna its1 region of western corn rootworm, diabrotica virgifera virgifera (wcr), and mexican corn rootworm, d. v. zeae (mcr) was studied. two sites were detected which differentiated wcr and mcr in the 642-base sequence. polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) analysis of the first internal transcribed spacer region (its1) sequence revealed no variation within or among the twelve wcr and two mcr populations. pcr-rflp of 75% o ... | 1999 | 10620046 |
| allozyme gene diversities in some leaf beetles (coleoptera: chrysomelidae). | gene diversity at allozyme loci was investigated in the bean leaf beetle, ceratoma trifurcata forster; the elm leaf beetle, xanthogaleruca luteola (muller); the cottonwood leaf beetle, chrysomela scripta fabricus; the western corn rootworm, diabrotica virgifera virgifera leconte; the southern corn rootworm, also called the spotted cucumber beetle, d. undecimpunctata howardi baker; the northern corn rootworm, d. barberi smith and lawrence; and the colorado potato beetle, leptinotarsa decemlineata ... | 1999 | 10624512 |
| lipid, polyamide, and flavonol phagostimulants for adult western corn rootworm from sunflower (helianthus annuus l.) pollen. | adult diabroticites including western corn rootworm (wcr), diabrotica virgifera virgifera leconte, consume pollen of corn, squash, sunflower, and other species. short-chain neutral amino acids in methanol-water extracts of pollen have been previously identified in our laboratory as strong phagostimulants for diabrotica. bioassay-driven fractionation was used to characterize the interacting lipid and midpolarity phagostimulants for adult wcr in giant gray stripe sunflower, helianthus annuus l., p ... | 1999 | 10552441 |
| interaction of insecticides, entomopathogenic nematodes, and larvae of the western corn rootworm (coleoptera: chrysomelidae). | chemical insecticides and entomopathogenic nematodes have been independently used to suppress corn rootworm damage in maize. we report on the mortality response of larvae of the western corn rootworm, diabrotica virgifera virgifera leconte, to the combined treatment with 1 of 3 insecticides (terbufos, fonofos, and tefluthrin) and the entomopathogenic nematode steinernema carpocapsae weiser (mexican strain). corn rootworm mortality with combinations of the insecticides terbufos or fonofos and s. ... | 1998 | 9589627 |
| behavioral and electrophysiological dose-response relationships in adult western corn rootworm (diabrotica virgifera virgifera leconte) for host pollen amino acids. | a strong correlation is shown between taste cell inputs and phagostimulatory outputs with predominant dietary pollen amino acids for western corn rootworm, diabrotica virgifera virgifera. behavioral and electrophysiological dose-response profiles in adult beetles are presented for five major free amino acids in host pollens. differential responses were found with strongest phagostimulation and sensory response elicited by l-alanine and l-serine, followed in order by l-proline and beta-alanine. g ... | 1998 | 12770166 |
| purification and kinetic analysis of acetylcholinesterase from western corn rootworm, diabrotica virgifera virgifera (coleoptera: chrysomelidae). | acetylcholinesterase (ache, ec 3.1.1.7) was purified from western corn rootworm (wcr, diabrotica virgifera virgifera) beetles by affinity chromatography. the purification factor reached over 20,000-fold with a specific activity of 169.5 mumol/min/mg and a yield of 23%. the vmax values for hydrolyzing acetylthiocholine (atc), acetyl-(beta-methyl) thiocholine (a beta mtc), propionylthiocholine (ptc), and s-butyrylthiocholine (btc) were 184.8, 140.5, 150.2, and 18.8 mumol/min/mg, respectively, and ... | 1998 | 9880902 |
| insecticidal activity of monoterpenoids to western corn rootworm (coleoptera: chrysomelidae), twospotted spider mite (acari: tetranychidae), and house fly (diptera: muscidae). | acute toxicities of 34 naturally occurring monoterpenoids were evaluated against 3 important arthropod pest species; the larva of the western corn rootworm, diabrotica virgifera virgifera leconte; the adult of the twospotted spider mite. tetranychus urticae koch; and the adult house fly. musca domestica l. potential larvicidal or acaricidal activities of each monoterpenoid were determined by topical application, leaf-dip method, soil bioassay, and greenhouse pot tests. phytotoxicity was also tes ... | 1997 | 9260540 |
| the role of wolbachia bacteria in reproductive incompatibilities and hybrid zones of diabrotica beetles and gryllus crickets. | a rickettsial bacterium in the genus wolbachia is the cause of a unidirectional reproductive incompatibility observed between two major beetle pests of maize, the western corn rootworm, diabrotica virgifera virgifera, and the mexican corn rootworm, d. v. zeae. these subspecies are allopatric except for two known regions of sympatry in texas and mexico. we demonstrate that populations of d. v. virgifera, with the exception of two populations in southern arizona, are infected with a strain of wolb ... | 1997 | 9326628 |
| identification of sensilla involved in taste mediation in adult western corn rootworm (diabrotica virgifera virgifera leconte). | a group of sensilla present on the maxillary galea of adult western corn rootworm,diabrotica virgifera virgifera leconte (coleoptera: chrysomelidae) beetles has been identified morphologically and physiologically to be involved in taste mediation. there are approximately 15 chemosensory hairs on each galea. bilateral removal of these structures resulted in a significantly reduced consumption of a strongly phagostimulant triterpenoid, cucurbitacin b, and led to increased ingestion of a phagodeter ... | 1995 | 24234063 |
| parasitism of western corn rootworm larvae and pupae by steinernema carpocapsae. | virulence and development of the insect-parasitic nematode, steinernema carpocapsae (weiser) (mexican strain), were evaluated for the immature stages of the western corn rootworm, diabrotica virgifera virgifera leconte. third instar rootworm larvae were five times more susceptible to nematode infection than second instar larvae and 75 times more susceptible than first instar larvae and pupae, based on laboratory bioassays. rootworm eggs were not susceptible. nematode development was observed in ... | 1995 | 19277256 |
| long-chain free fatty acids: semiochemicals for host location by western corn rootworm larvae. | a bioassay-driven sequential fractionation scheme was used to isolate fractions of a crude dichloromethane maize seedling extract behaviorally active to larvae of the western corn rootworm,diabrotica virgifera virgifera leconte. (z,z)-9,12-octadecadienoic (linoleic) acid, (z)-9-octadecenoic (oleic) acid, and octadecanoic (stearic) acid were identified from a purified fraction of maize extract that was attractive to western corn rootworm larvae in choice tests with equal levels of carbon dioxide ... | 1994 | 24241996 |
| effects of pesta-pelletized steinernema carpocapsae (all) on western corn rootworms and colorado potato beetles. | pesta-pelletized steinernema carpocapsae (all) nematodes were used in soil treatments in the greenhouse against larvae of western corn rootworm and prepupae of colorado potato beetle. the pesta-pellets delivered 100,000 living nematodes/g. infective-stage nematodes and their associated bacteria survived the pesta-pellet process, emerged from the pellets in large numbers in the soil, and reduced adult emergence of both pest insects by more than 90%. | 1994 | 19279889 |
| distribution and antifeedant associations of sesquiterpene lactones in cultivated sunflower (helianthus annuus l.) on western corn rootworm (diabrotica virgifera virgifera leconte). | seven antifeedant sesquiterpene lactones (stls), 4,5-dihydroniveusin a, argophyllin b, argophyllin a, 15-hydroxy-3-dehydrodesoxytifruticin, niveusin b, 1,2-anhydridoniveusin a, and an unidentified epoxide, in cultivated sunflower (helianthus annuus l.) have been quantified by a highperformance thin-layer chromatography and uv-reflectance scanning densitometry analysis. age-related expression of stl content in sunflower reveals a heretofore undescribed pattern in which nonpolar stls such as 15-hy ... | 1993 | 24249174 |
| 6-methoxy-2-benzoxazolinone: a semiochemical for host location by western corn rootworm larvae. | a bioassay-driven sequential fractionation scheme was used to isolate all portions of a crude dichloromethane corn seedling extract behaviorally active to larvae of the western corn rootworm,diabrotica virgifera virgifera leconte. 6-methoxy-2-benzoxazolinone (mboa) was identified as one of the most important components of an attractive crude corn extract. mboa was found on or in the intact root tissues by injecting an extract of undamaged roots onto an hplc immediately after extraction. mboa was ... | 1992 | 24254139 |
| evolution of diabroticite rootworm beetle (chrysomelidae) receptors for cucurbita blossom volatiles. | the diabroticite rootworm beetles coevolved with plants of the family cucurbitaceae as demonstrated by their feeding dependence on the tetracyclic triterpenoid cucurbitacins. these beetles also exhibit strong attraction to phenylpropanoid volatile components of cucurbita blossoms. a mixture of 1,2,4-trimethoxybenzene, indole, and (e)-cinnamaldehyde, all blossom components, is highly attractive to the several species of diabroticite cucumber beetles and corn rootworms and is considered a simplifi ... | 1991 | 11607158 |
| behavioral responses of western corn rootworm larvae to volatile semiochemicals from corn seedlings. | corn seedling volatiles collected cryogenically are highly attractive to western corn rootworm larvae,diabrotica virgifera virgifera leconte (coleoptera: chrysomelidae), in a laboratory bioassay. carbon dioxide is known as an attractant for western corn rootworm larvae, and the amount of carbon dioxide in the cryogenic collections was measured with an infrared gas analyzer. in a choice test between a source containing carbon dioxide alone and a source containing corn seedling volatiles with an e ... | 1988 | 24276403 |
| identification of a female-produced sex pheromone of the western corn rootworm. | a sex pheromone has been isolated and identified from virgin females of the western corn rootworm (wcr),diabrotica virgifera virgifera leconte. the synthesized compound, racemic 8-methyl-2-decanol propanoate, was equal in attraction to the natural pheromone when tested in the field as a trap bait against three taxa ofdiabrotica known to respond to pheromone extracts from female wcr. five taxa (d. virgifera virgifera; d. virgifera zeae krysan and smith, mexican corn rootworm;d. longicornis barber ... | 1982 | 24414965 |
| negative phototactic activity used to determine larval toxicity of insecticides to diabrotica virgifera. | 1975 | 1141486 | |
| rna interference as a method for target-site screening in the western corn rootworm, diabrotica virgifera virgifera. | to test the efficacy of rna interference (rnai) as a method for target-site screening in diabrotica virgifera virgifera leconte (coleptera: chrysomelidae) larvae, genes were identified and tested for which clear rnai phenotypes had been identified in the coleopteran model, tribolium castaneum. here the cloning of the d. v. vergifera orthologs of laccase 2 (dvvlac2) and chitin synthase 2 (dvvchs2) is reported. injection of dvvlac2-specific double-stranded rna resulted in prevention of post-molt c ... | 0 | 21067417 |