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stenotrophomonas maltophilia pacemaker endocarditis in a patient with d-transposition of the great arteries after atrial switch procedure.we report a case of pacemaker endocarditis due to stenotrophomonas maltophilia in a 22-year-old caucasian man with d-transposition of the great arteries after atrial switch procedure. s.maltophilia isolated from blood cultures was susceptible to trimethoprim-sulfamethoxazole and amikacin, and resistant to ciprofloxacin and all tested β-lactam antibiotics. the infected pacemaker system was completely removed by thoracotomy. simultaneously, a new ddd pacemaker and epicardial electrodes were succes ...201019171390
sterilization of liquid nitrogen with ultraviolet irradiation for safe vitrification of human oocytes or embryos.to evaluate the efficacy of ultraviolet (uv) irradiation for rapid microbial decontamination of liquid nitrogen (ln2).201019591992
stenotrophomonas ginsengisoli sp. nov., isolated from a ginseng field.a gram-negative, non-spore-forming, rod-shaped bacterium, designated strain dcy01(t), was isolated from soil from a ginseng field in south korea and was characterized in order to determine its taxonomic position. 16s rrna gene sequence analysis revealed that strain dcy01(t) belonged to the gammaproteobacteria and was most closely related to stenotrophomonas koreensis kctc 12211(t) (98.4 % similarity), stenotrophomonas humi r-32729(t) (97.2 %), stenotrophomonas terrae r-32768 (97.1 %), stenotroph ...201019684314
point-of-use water filtration reduces healthcare-associated infections in bone marrow transplant recipients.outbreaks of infection with gram-negative bacteria (gnb) have been linked to hospital water. we sought to determine whether point-of-use (pou) water filtration might result in decreased risk of infection in hospitalized bone marrow transplant (bmt) recipients in the absence of any recognized outbreak. unfiltered water was sampled from taps in the bmt unit of a major us teaching hospital, and cultured at a reference laboratory. pou bacterial-retentive filters (0.2 mum) were installed throughout t ...201019781018
purification and characterization of 3-ketovalidoxylamine a c-n lyase produced by stenotrophomonas maltrophilia.a soluble 3-ketovalidoxylamine a c-n lyase from stenotrophomonas maltrophilia was purified to 367.5-fold from the crude enzyme, with a yield of 16.4% by column chromatography on high s iex, methyl hic, high q iex, and sephadex g 100. the molecular mass of the enzyme was estimated to be 34 kda by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the enzyme was a neutral protein having an isoelectric point value at ph 7.0. the optimal ph of 3-ketovalidoxylamine a c-n lyase was around ...201019795222
stenotrophomonas maltophilia bacteraemia in 40 haematology patients: risk factors, therapy and outcome. 201019802036
in vitro pharmacodynamics of levofloxacin and other aerosolized antibiotics under multiple conditions relevant to chronic pulmonary infection in cystic fibrosis.the inhalational administration of antibiotics can provide high concentrations locally in the lungs of cystic fibrosis patients and, thus, can be useful for the treatment of chronic bacterial infections. the present study evaluated the in vitro activities of levofloxacin, ciprofloxacin, tobramycin, amikacin, and aztreonam against clinical isolates of pseudomonas aeruginosa, burkholderia cepacia complex, stenotrophomonas maltophilia, alcaligenes xylosoxidans, and staphylococcus aureus from cystic ...201019805554
risk factors for relapse of ventilator-associated pneumonia in trauma patients.our goal was to define risk factors for ventilator-associated pneumonia (vap) relapse and examine the implications, if any, for initial therapy in trauma patients.200919590315
antibacterial activity of synthetic fire ant venom: the solenopsins and isosolenopsins.we determined the in vitro activity of 9 synthetic fire ant venom alkaloids (+/-)-solenopsin a, (2r, 6r)-solenopsin a, (2s, 6s)-solenopsin b, (+/-)-isosolenopsin a, (2s, 6r)-isosolenopsin a,(2r, 6s)-isosolenopsin a, (+/-)-isosolenopsin b, (2s, 6r)-isosolenopsin b, and (2r, 6s)-isosolenopsin b against 6 species of bacteria (streptococcus pneumoniae, staphylococcus aureus, enterococcus faecalis, escherichia coli, stenotrophomonas maltophilia, and pseudomonas aeruginosa).200919826319
dicamba monooxygenase: structural insights into a dynamic rieske oxygenase that catalyzes an exocyclic monooxygenation.dicamba (2-methoxy-3,6-dichlorobenzoic acid) o-demethylase (dmo) is the terminal rieske oxygenase of a three-component system that includes a ferredoxin and a reductase. it catalyzes the nadh-dependent oxidative demethylation of the broad leaf herbicide dicamba. dmo represents the first crystal structure of a rieske non-heme iron oxygenase that performs an exocyclic monooxygenation, incorporating o(2) into a side-chain moiety and not a ring system. the structure reveals a 3-fold symmetric trimer ...200919616009
in vitro study of antibacterial activity on multi-resistant bacteria and chemical composition of the chloroform extract of endemic centaurea drabifolia subsp. cappadocica.the antimicrobial activity of the n-hexane, chloroform, ethyl acetate and ethanol extracts of the aerial parts of c. drabifolia s.m. subsp. cappadocica (dc.) wagenitz (asteraceae) was evaluated against microorganisms including multi-antibiotic resistant bacteria using the paper disc diffusion method. the chemical composition of the chloroform extract of this plant was determined by gas chromatography and gas chromatography-mass spectrometry. the chloroform extract exhibited significant antibacte ...200919831042
characterization of an isoproturon mineralizing bacterial culture enriched from a french agricultural soil.the phenylurea herbicide isoproturon, 3-(4-isopropylphenyl)-1,1-dimethylurea (ipu), was found to be rapidly mineralized by a bacterial culture isolated from an agricultural soil regularly exposed to ipu. molecular analysis of the bacterial culture by dna fingerprinting, cloning and sequencing of the 16s rrna genes revealed that it consisted of six different members among whom the dominant was related to sphingomonas sp. six bacterial strains belonging to genera ancylobacter, pseudomonas, stenotr ...200919836052
a novel bacterial isolate stenotrophomonas maltophilia as living factory for synthesis of gold nanoparticles.abstract:200919619318
crystallization and preliminary x-ray diffraction characterization of rpff, a key dsf synthase from stenotrophomonas maltophilia.stenotrophomonas maltophilia has emerged as a critical nosocomial opportunistic pathogen in the last few years. it is resistant to many clinically useful antibiotics; hence, new ways of combatting this bacterium are essential. diffusible signal factor (dsf) dependent quorum sensing is a major mechanism of virulence induction in s. maltophilia, with rpff playing a key role in dsf biosynthesis. inhibiting s. maltophilia rpff (smrpff) function via small-molecule interference may constitute a new wa ...200919851012
risk factors for mortality in patients with nosocomial stenotrophomonas maltophilia pneumonia.the aim of this study was to determine potential risk factors for mortality in patients with nosocomial stenotrophomonas maltophilia pneumonia.200919852664
multidrug resistant gram-negative bacilli as predominant bacteremic pathogens in liver transplant recipients.bacteremias, which are often caused by gram-negative bacteria, are the most frequently occurring infectious complications after liver transplantation (lt). the aim of this study was to investigate bacteremic incidence, pathogenic spectrum, risk factors for bacteremia due to multidrug resistant (mdr) gram-negative bacilli, and its impact on mortality after lt.200919638006
a novel isoquinoline alkaloid, dd-carboxypeptidase inhibitor, with antibacterial activity isolated from streptomyces sp. 8812. part i: taxonomy, fermentation, isolation and biological activities.a novel isoquinoline alkaloid of molecular formula c10h9no4, labeled js-1, was isolated from the culture broth of streptomyces sp. 8812. it was purified by acetone protein precipitation from the culture supernatant, followed by anion exchange and c18 rp hplc columns. js-1 is an inhibitor of exocellular dd-carboxypeptidases/transpeptidases (dd-peptidases) 64-575 ii from saccharopolyspora erythraea 64-575 ii, and r39 from actinomadura r39. js-1 exhibits activity against gram-negative bacteria, suc ...200919713994
hydroxylation of thiacloprid by bacterium stenotrophomonas maltophilia cgmcc1.1788.chloropyridinyl neonicotinoid insecticides play a major role in crop protection and flea control on cats and dogs. imidacloprid, thiacloprid and acetamiprid have in common the 6-chloro-3-pyridinylmethyl group but differ in the nitroguanidine or cyanoamidine substituent on an acyclic or cyclic moiety. our previous study found that stenotrophomonas maltophilia cgmcc 1.1788 could hydroxylate imidacloprid to 5-hydroxy imidacloprid, and 5-hydroxy imidacloprid was easily converted to 10-19 times highe ...200919421875
bacterial contamination of patients' medical charts in a surgical ward and the intensive care unit: impact on nosocomial infections.the purpose of this study was to determine the degree of bacterial contamination of patients' files, and to compare the colonized bacteria between files from the surgical intensive care unit (icu) and the surgical ward at the wan fang hospital, taipei, taiwan.200919424563
trends in pathogens colonising the respiratory tract of adult patients with cystic fibrosis, 1985-2005.the treatment of patients with cf has continued to evolve. we hypothesised that sputum microbiology may have changed as a result of this.200919740710
multidrug-resistant gram-negative infections: what are the treatment options?the emergence of multidrug-resistant (mdr) gram-negative bacilli creates a challenge in the treatment of nosocomial infections. while the pharmaceutical pipeline is waning, two revived old antibacterials (colistin and fosfomycin), a newer one (tigecycline) and an 'improved' member of an existing class (doripenem) are the only therapeutic options left. the class of polymyxins, known since 1947 and represented mostly by polymyxin b and polymyxin e (colistin), has recently gained a principal role i ...200919747006
impact of cadmium on the bacterial communities in the gut of metaphire posthuma.the effects of cadmium (cd) contamination in soil onto the bacterial communities of the guts pooled from ten metaphire posthuma were addressed during 14 days' incubation. we found that about 50% of cd (5mg/kg, dry weight soil) in the contaminated soil was bio-accumulated into the earthworms. dna was extracted from the guts of m. posthuma and their dwelling soil irrespective of cd treatment for the analysis of the bacterial communities of guts in m. posthuma and in soil by pcr-dgge (polymerase ch ...200919747774
prevalence and antimicrobial susceptibility of extended-spectrum beta-lactamase-producing bacteria in intensive care units of sanandaj general hospitals (kurdistan, iran).this study focused on analyzing the spread of extended-spectrum beta-lactamase (esbl) enzymes among gram-negative bacteria at intensive care units (icus). between january 2007 and january 2008, 301 consecutive clinical isolates of gram-negative type were isolated. of these, 66 strains were collected from patients in icus in two major hospitals in sanandaj (kurdistan, iran). the isolates were identified, tested for antimicrobial susceptibility, and analyzed for the presence of esbl using the doub ...200919521074
the antibacterial properties of malaysian tualang honey against wound and enteric microorganisms in comparison to manuka honey.antibiotic resistance of bacteria is on the rise, thus the discovery of alternative therapeutic agents is urgently needed. honey possesses therapeutic potential, including wound healing properties and antimicrobial activity. although the antimicrobial activity of honey has been effectively established against an extensive spectrum of microorganisms, it differs depending on the type of honey. to date, no extensive studies of the antibacterial properties of tualang (koompassia excelsa) honey on wo ...200919754926
stenotrophomonas maltophilia endogenous endophthalmitis: clinical presentation, sensitivity spectrum and management.stenotrophomonas maltophilia is gaining importance as a community-acquired pathogen, after becoming firmly established as a nosocomial pathogen. here we report a case of endogenous endophthalmitis due to s. maltophilia. antibiotic-susceptibility testing of the isolate was performed by the kirby-bauer disc diffusion method. the organism was resistant to aminoglycosides, imipenem, ticarcillin and cotrimoxazole and was sensitive to ceftazidime and chloramphenicol. the patient was successfully treat ...200919429764
[antimicrobial resistance of clinical isolates of stenotrophomonas maltophilia].to investigate the antimicrobial resistance of clinical isolates of stenotrophomonas matophilia (sma) and the mechanisms of their drug resistance.200919460691
simultaneous degradation of organophosphates and 4-substituted phenols by stenotrophomonas species lz-1 with surface-displayed organophosphorus hydrolase.organophosphorous hydrolase (oph) was expressed onto the surface of a stenotrophomonas species (lz-1), capable of simultaneously degrading 4-substituted phenols, using the n- and c-terminal domains of ice nucleation protein (inpnc) as an anchoring motif for the first time. the engineered strain lz-1 could degrade p-nitrophenyl-substituted organophosphates as well as their hydrolytic product, pnp, rapidly. especially, addition of 4-cp (below 0.8 mm) significantly accelerated the complete degradat ...200919548671
stenotrophomonas maltophilia isolated from the airways of animals with chronic respiratory disease.stenotrophomonas maltophilia (s. maltophilia) is a nonfermentative bacterium, which is naturally resistant against a panel of commonly-used antibiotics. it is frequently isolated from humans with chronic respiratory disease, e.g. cystic fibrosis or chronic obstructive pulmonary disease. in veterinary medicine s. maltophilia is perceived to be a mere coloniser. we herewith report 7 strains of s. maltophilia isolated from animals, of which 5 strains were harvested from 3 horses, a dog and a cat wi ...200919565454
comparison of two multimetal resistant bacterial strains: enterobacter sp. ysu and stenotrophomonas maltophilia oro2.the y-12 plant in oak ridge, tn, which manufactured nuclear weapons during world war ii and the cold war, contaminated east fork poplar creek with heavy metals. the multimetal resistant bacterial strain, stenotrophomonas maltophilia oak ridge strain o2 (s. maltophilia o2), was isolated from east fork poplar creek. sequence analysis of 16s rdna suggested that our working strain of s. maltophilia o2 was a strain of enterobacter. phylogenetic tree analysis and biochemical tests confirmed that it be ...200919688378
antibacterial activities of a fosfomycin/tobramycin combination: a novel inhaled antibiotic for bronchiectasis.to compare the in vitro and in vivo activities of a 4:1 (w/w) fosfomycin/tobramycin combination (fti) with those of fosfomycin and tobramycin alone against cystic fibrosis (cf) and non-cf bronchiectasis pathogens.200919679597
activity of levofloxacin and ciprofloxacin on biofilms and planktonic cells of stenotrophomonas maltophilia isolates from patients with device-associated infections.the aim of this work was to compare the in vitro effects of levofloxacin and ciprofloxacin on pre-formed biofilms and planktonic populations of stenotrophomonas maltophilia isolates from device-associated infections recovered over the period 2004-2008 at a university hospital in argentina. for planktonic susceptibility studies, the minimum inhibitory concentrations (mics) of fluoroquinolones were determined by the broth microdilution method. from the 32 isolates recovered, 84.4% were sensitive t ...200919362452
morphological evidence of biofilm formation in greenlanders with chronic suppurative otitis media.biofilm may explain the recurrences and recalcitrant episodes of otorrhea in chronic suppurative otitis media (csom). this study investigates bacterial biofilm in greenlanders with csom and chronic otitis media with effusion (come). the study is partly blinded, prospective and retrospective. six children with csom, four children with come and ten adults with csom were included in this study. cultures were obtained and examined by standard methods. otorrhea or glue was collected from the children ...200919283404
molecular cloning and characterization of smra, a novel abc multidrug efflux pump from stenotrophomonas maltophilia.stenotrophomonas maltophilia is an emerging nosocomial pathogen that can cause difficult-to-treat infections and exhibits significant degrees of poorly understood multidrug resistance (mdr). the aim of this study was to identify and characterize a multidrug atp-binding cassette (abc) efflux pump in s. maltophilia.200919643774
incidence and risk factors of early bacterial infections after unrelated cord blood transplantation.incidence and characteristics of early bacterial infection within 100 days after unrelated cord blood transplantation (ucbt) were assessed for 664 pediatric and 1208 adult recipients in japan. cumulative incidence of early bacterial infection at day 100 post-ucbt was 11% (95% confidence interval [ci], 8%-13%) for children and 21% (ci, 19%-24%) for adults (p < .0001). early bacterial infection in adults had a significant impact on mortality (hazard ratio [hr] = 2.1, ci, 1.7-2.6; p < .0001), altho ...200919285631
structural and functional analysis of smet, the repressor of the stenotrophomonas maltophilia multidrug efflux pump smedef.stenotrophomonas maltophilia is an opportunistic pathogen characterized for its intrinsic low susceptibility to several antibiotics. part of this low susceptibility relies on the expression of chromosomally encoded multidrug efflux pumps, with smedef being the most relevant antibiotic resistance efflux pump so far studied in this bacterial species. expression of smedef is down-regulated by the smet repressor, encoded upstream smedef, in its complementary dna strand. in the present article we pre ...200919324881
antimicrobial activity of three tick defensins and four mammalian cathelicidin-derived synthetic peptides against lyme disease spirochetes and bacteria isolated from the midgut.in this study, chemically synthesized tick defensins and cathelicidin-derived mammalian peptides were used to investigate the activity spectrum against borrelia garinii and symbiotic stenotrophomonas maltophila. synthetic tick defensins showed antimicrobial activity against staphylococcus aureus but not b. garinii and s. maltophila. mammalian peptides which have cationic property similar to tick defensins, showed antimicrobial activity similar to tick defensins. the antimicrobial peptides in tic ...200919229642
crystal structure of dicamba monooxygenase: a rieske nonheme oxygenase that catalyzes oxidative demethylation.dicamba (3,6-dichloro-2-methoxybenzoic acid) is a widely used herbicide that is efficiently degraded by soil microbes. these microbes use a novel rieske nonheme oxygenase, dicamba monooxygenase (dmo), to catalyze the oxidative demethylation of dicamba to 3,6-dichlorosalicylic acid (dcsa) and formaldehyde. we have determined the crystal structures of dmo in the free state, bound to its substrate dicamba, and bound to the product dcsa at 2.10-1.75 a resolution. the structures show that the dmo act ...200919616011
bacterial contamination of cystic fibrosis clinics.respiratory pathogens from cf patients can contaminate inpatient settings, which may be associated with increased risk of patient-to-patient transmission. few data are available that assess the rate of bacterial contamination of outpatient settings. we determined the frequency of contamination of cf clinics and the effectiveness of alcohol-based disinfectants in reducing hand carriage of bacterial pathogens.200919250885
tigecycline: a new treatment option for intra-abdominal infections.tigecycline is the first of a new class of antibiotics, the glycylcyclines, with properties which can overcome many common resistance mechanisms, making it appropriate for treatment of many serious and life-threatening infections for which other antibiotics are no longer appropriate. its wide antibacterial spectrum includes most methicillin-resistant staphylococci, vancomycin-resistant enterococci, esbl-producing and other multi-resistant gram-negative bacteria such as acinetobacter and stenotro ...200919622449
early- and late-onset pneumonia: is this still a useful classification?the choice of empirical treatment of nosocomial pneumonia in the intensive-care unit (icu) used to rely on the interval after the start of mechanical ventilation. nowadays, however, the question of whether in fact there is a difference in the distribution of causative pathogens is under debate. data from 308 icus from the german national nosocomial infection surveillance system, including information on relevant pathogens isolated in 11,285 cases of nosocomial pneumonia from 1997 to 2004, were u ...200919364852
control of pseudomonas aeruginosa and stenotrophomonas maltophilia contamination of microfiltered water dispensers with peracetic acid and hydrogen peroxide.the abilities of peracetic acid and hydrogen peroxide to remove or reduce pseudomonas aeruginosa and stenotrophomonas maltophilia in output water from microfiltered water dispensers (mwds) were investigated. two mwds were inoculated with strains of p. aeruginosa and s. maltophilia isolated from water. dispensers a and b were disinfected with 10% (v/v) peracetic acid (paa) and 3% (v/v) hydrogen peroxide (hp) respectively. each dispenser was disinfected three times at monthly intervals with contac ...200919439386
prevalence of infectious pathogens in crohn's disease.the importance of infectious pathogens in crohn's disease (cd) is still under debate. therefore, we examined a panel of potential viral and bacterial pathogens in a large series of cd patients and controls. archival tissue from 76 patients, 56 with cd and 20 control patients, with normal colon mucosa (n=10) and non-steroid anti-inflammatory drug (nsaid)-induced colitis (n=10) were examined using pcr-based detection methods for human cytomegalovirus (cmv), epstein-barr virus (ebv), herpes simplex ...200919186006
susceptibility of 228 non-fermenting gram-negative rods to tigecycline and six other antimicrobial drugs.the aim of the study was to determine the in vitro activity of tigecycline and 6 other antimicrobial drugs used in clinical practice against 228 clinical isolates of nonfermenting gram-negative rods (nfgnrs) including acinetobacter spp., stenotrophomonas maltophilia, and pseudomonas aeruginosa. minimum inhibitory concentrations (mics) were determined according to the recommendations of the clinical and laboratory standards institute. for tigecycline, we used the criteria approved by the fda. alm ...200919567346
successful treatment of stenotrophomonas maltophilia soft tissue infection with tigecycline: a case report. 200919567363
investigation of bacterial pathogens on 70 frequently used environmental surfaces in a large urban u.s. university.after reports of increased severity of bacterial infections from community institutions, a broad spectrum of 70 surfaces was sampled for potential bacterial pathogens in the morning and afternoon of one day per week over three consecutive weeks in a large u.s. university. surfaces included public telephone mouthpieces, water fountain drains, student computer keyboards and desks, and buttons on elevators, vending machines, and photocopiers. a total of 420 samples was obtained. bacterial counts we ...200919192740
broad-range pcr, cloning and sequencing of the full 16s rrna gene for detection of bacterial dna in synovial fluid samples of tunisian patients with reactive and undifferentiated arthritis.broad-range rdna pcr provides an alternative, cultivation-independent approach for identifying bacterial dna in reactive and other form of arthritis. the aim of this study was to use broad-range rdna pcr targeting the 16s rrna gene in patients with reactive and other forms of arthritis and to screen for the presence of dna from any given bacterial species in synovial fluid (sf) samples.200919570210
current status of newer carbapenems.beta-lactam antibiotics represent the most commonly prescribed antibacterial agents. since many bacteria have developed resistance to older agents, new beta-lactams have been introduced constantly. in the late 1970s, a new class of exceptionally broad-spectrum beta-lactams, the carbapenems, was identified. the carbapenems, such as the most popular imipenem and meropenem, have the widest spectra of antibacterial activity of all the beta-lactams and provide excellent coverage of many gram-negative ...200919199922
cough-generated aerosols of pseudomonas aeruginosa and other gram-negative bacteria from patients with cystic fibrosis.pseudomonas aeruginosa is the most common bacterial pathogen in patients with cystic fibrosis (cf). current infection control guidelines aim to prevent transmission via contact and respiratory droplet routes and do not consider the possibility of airborne transmission. it was hypothesised that subjects with cf produce viable respirable bacterial aerosols with coughing.200919574243
molecular characterization of stenotrophomonas maltophilia isolates from cystic fibrosis patients and the hospital environment.to ascertain whether cystic fibrosis (cf) patients are colonized or infected with unique or multiple strains of stenotrophomonas maltophilia; to understand whether some strains colonize or infect more than 1 patient, indicating clonal spread; and to explore the molecular heterogeneity of hospital water isolates and their correlation with clinical isolates.200919583515
bsac standardized disc susceptibility testing method (version 8).there have been considerable changes to the format of the recommendations since the previous version (version 7). the majority of the footnotes to the tables have been removed and the notations added to the end column; it is hoped that this change will avoid confusion in interpretation. antibiotics have been separated into groups, e.g. beta-lactams, aminoglycosides, etc. recommendations for urinary tract infections (utis) have been removed for most agents except for those that are administered s ...200919587067
association between contaminated faucets and colonization or infection by nonfermenting gram-negative bacteria in intensive care units in taiwan.this study was designed to determine the strength of the association between the isolation of nonfermentative gram-negative bacilli (nfgnb) from tap water faucet aerators and the prevalence of colonization or infection of patients in intensive care units (icus). surveillance cultures were obtained during a 4-month period from 162 faucet aerators located in seven different icus. the prevalence of colonization or infection of icu patients with nfgnb was determined by prospective surveillance durin ...200919587299
structure and mechanism of copper- and nickel-substituted analogues of metallo-beta-lactamase l1.in an effort to further probe metal binding to metallo-beta-lactamase l1 (mbetal l1), cu- (cu-l1) and ni-substituted (ni-l1) l1 were prepared and characterized by kinetic and spectroscopic studies. cu-l1 bound 1.7 equiv of cu and small amounts of zn(ii) and fe. the epr spectrum of cu-l1 exhibited two overlapping, axial signals, indicative of type 2 sites with distinct affinities for cu(ii). both signals indicated multiple nitrogen ligands. despite the expected proximity of the cu(ii) ions, howev ...200919228020
selenite resistant rhizobacteria stimulate seo(3) (2-) phytoextraction by brassica juncea in bioaugmented water-filtering artificial beds.selenium is a trace metalloid of global environmental concern. the boundary among its essentiality, deficiency, and toxicity is narrow and mainly depends on the chemical forms and concentrations in which this element occurs. different plant species-including brassica juncea-have been shown to play a significant role in se removal from soil as well as water bodies. furthermore, the interactions between such plants, showing natural capabilities of metal uptake and their rhizospheric microbial comm ...200919104867
diagnosis and antimicrobial therapy of lung infiltrates in febrile neutropenic patients: guidelines of the infectious diseases working party of the german society of haematology and oncology.patients with neutropenia lasting for more than 10d, who develop fever and pulmonary infiltrates, are at risk of treatment failure under conventional broad-spectrum antibacterial therapy. filamentous fungi are predominant causes of failure, however, multi-resistant gram-negative rods such as pseudomonas aeruginosa or stenotrophomonas maltophilia may be involved. prompt addition of mould-active systemic antifungal therapy, facilitated by early thoracic computed tomography, improves clinical outco ...200919467584
isolation and genetic identification of pah degrading bacteria from a microbial consortium.polycyclic aromatic hydrocarbons (pah; naphthalene, anthracene and phenanthrene) degrading microbial consortium c2pl05 was obtained from a sandy soil chronically exposed to petroleum products, collected from a petrochemical complex in puertollano (ciudad real, spain). the consortium c2pl05 was highly efficient degrading completely naphthalene, phenanthrene and anthracene in around 18 days of cultivation. the toxicity (microtox method) generated by the pah and by the intermediate metabolites was ...200919468841
design and quality control of a pharmaceutical formulation containing natural products with antibacterial, antifungal and antioxidant properties.the aims of the present study were to determine the antibacterial and antifungal activity as well as mutagenicity of sechium edule fluid extract and to obtain a pharmaceutical formulation with them. the extract exhibited antimicrobial activity against escherichia coli, klebsiella pneumoniae, proteus mirabilis, enterobacter cloacae, serratia marcescens, morganella morganii, acinetobacter baumannii, pseudomonas aeruginosa, stenotrophomonas maltophilia, candida spp. and aspergillus spp. isolated fr ...200919477252
extremophile culture collection from andean lakes: extreme pristine environments that host a wide diversity of microorganisms with tolerance to uv radiation.a total of 88 bacterial strains were isolated from six andean lakes situated at altitudes ranging from 3,400 to 4,600 m above sea level: l. aparejos (4,200 m), l. negra (4,400 m), l. verde (4,460 m), l. azul (4,400 m), l. vilama (4,600 m), and salina grande (3,400 m). salinity ranged from 0.4 to 117 ppm. general diversity was determined by denaturing gradient gel electrophoresis (dgge) analysis. from the excised dgge bands, 182 bacterial sequences of good quality were obtained. gammaproteobacter ...200919495855
respiratory microbiology of patients with cystic fibrosis in the united states, 1995 to 2005.numerous improvements in diagnostic and therapeutic strategies for patients with cystic fibrosis (cf) have occurred during the past 2 decades. we hypothesized that these changes could impact trends in respiratory microbiology.200919505987
efficacy of bacterial consortium-aie2 for contemporaneous cr(vi) and azo dye bioremediation in batch and continuous bioreactor systems, monitoring steady-state bacterial dynamics using qpcr assays.bacterial consortium-aie2 with a capability of contemporaneous cr(vi) reduction and azo dye rv5 decolourization was developed from industrial wastewaters by enrichment culture technique. the 16s rrna gene based molecular analyses revealed that the consortium bacterial community structure consisted of four bacterial strains namely, alcaligenes sp. dma, bacillus sp. dmb, stenotrophomonas sp. dms and enterococcus sp. dme. cumulative mechanism of cr(vi) reduction by the consortium was determined usi ...200919517254
probable trimethoprim/sulfamethoxazole-induced higher-level gait disorder and nocturnal delirium in an elderly man.to report a case of probable trimethoprim/sulfamethoxazole (tmp/smx)-induced higher-level gait disorder (hlgd) and nocturnal delirium in an elderly patient on high-dose oral therapy.200919109207
melanonychia caused by stenotrophomonas maltophilia. 200919120402
carbon utilization profiles of bacteria colonizing the headbox water of two paper machines in a canadian mill.forty-one bacterial strains isolated from the headbox water of two machines in a canadian paper mill were associated with the genera asticcacaulis, acidovorax, bacillus, exiguobacterium, hydrogenophaga, pseudomonas, pseudoxanthomonas, staphylococcus, stenotrophomonas based on the sequence of their 16s rrna genes. the metabolic profile of these strains were determined using biolog ecoplate, and the bacteria were divided into four metabolic groups. metabolic profiles of the bacterial communities c ...200919137341
characterization of a novel stenotrophomonas isolate with high keratinase activity and purification of the enzyme.a feather-degrading bacterium was isolated from poultry decomposition feathers in china. the strain, named l1, showed significant feather-degrading activity because it grew and reproduced quickly on basal medium containing 10 g/l of native feather as the source of energy, carbon, and nitrogen. according to the phenotypic characteristics and 16s rrna profile, the isolate belongs to stenotrophomonas maltophilia. keratinase activity of the isolate was determined during cultivation on raw feathers a ...200919137342
enhanced degradation of tnt by genome-shuffled stenotrophomonas maltophilia ok-5.in this study, the enhanced degradation of tnt using cultures of genome-shuffled stenotrophomonas maltophilia ok-5 mt-3 has been examined and the proteome of shuffled strain was compared to the wild-type ok-5 strain. genome shuffling of s. maltophilia ok-5 was used to achieve a rapid enhancement of tnt degradation. the initial mutant population was generated by ntg treatment and uv irradiation. the wild-type ok-5 strain was able to degrade 0.2 mm tnt within 6 days, yet barely tolerated 0.5 mm tn ...200919543944
metabolism of acenaphthylene via 1,2-dihydroxynaphthalene and catechol by stenotrophomonas sp. rmsk.stenotrophomonas sp. rmsk capable of degrading acenaphthylene as a sole source of carbon and energy was isolated from coal sample. metabolites produced were analyzed and characterized by tlc, hplc and mass spectrometry. identification of naphthalene-1,8-dicarboxylic acid, 1-naphthoic acid, 1,2-dihydroxynaphthalene, salicylate and detection of key enzymes namely 1,2-dihydroxynaphthalene dioxygenase, salicylaldehyde dehydrogenase and catechol-1,2-dioxygenase in the cell free extract suggest that a ...200919543983
development of an autofluorescent organophosphates-degrading stenotrophomonas sp. with dehalogenase activity for the biodegradation of hexachlorocyclohexane (hch).simultaneous biodegradation of hexachlorocyclohexane (hch) and organophosphates (ops) by a recombinant stenotrophomonas sp. was studied in the study. the broad-host-range plasmid pvgab, harboring enhanced green fluorescent protein gene (egfp) and dehalogenase genes (lina and linb), was constructed and transformed into the op-degrading strain stenotrophomonas yc-1 to get the recombinant strain yc-h. over-expression of dehalogenase (lina and linb) and enhanced green fluorescent protein (egfp) was ...200919269809
clinical investigation of isolated bacteria from urinary tracts of hospitalized patients and their susceptibilities to antibiotics.complicated urinary tract infections (utis) are often difficult to treat, partly because of the existence of isolated antibiotic-resistant strains. even though the definition of uti is determined by the quantity of cultured bacteria, it has been unclear if the quantity of cultured uti bacteria affects their susceptibility to antimicrobial agents. also, the gram stain is generally performed to assume the causative bacteria and their quantity before culture results can be obtained; therefore, we c ...200919280295
factors associated with bacteremia due to multidrug-resistant gram-negative bacilli in hematopoietic stem cell transplant recipients.the epidemiology of bacteremia developing during neutropenia has changed in the past decade, with the re-emergence of gram-negative (gn) bacteria and the development of multidrug resistance (mdr) among gn bacteria. we conducted a case-control study in order to identify factors associated with bacteremia due to multidrug-resistant gram-negative (mdrgn) isolates in hematopoietic stem cell transplant recipients. ten patients with mdrgn bacteremia were compared with 44 patients with gn bacteremia wi ...200919287908
sec- and tat-dependent translocation of beta-lactamases across the escherichia coli inner membrane.beta-lactamases represent the major resistance mechanism of gram-negative bacteria against beta-lactam antibiotics. the amino acid sequences of these proteins vary widely, but all are located in the periplasm of bacteria. in this study, we investigated the translocation mechanism of representative beta-lactamases in an escherichia coli model. n-terminal signal sequence analyses, antibiotic activity assay, and direct measurement of translocation of a green fluorescent protein (gfp) reporter fused ...200918981261
evaluation of the vitek 2 cards for identification and antimicrobial susceptibility testing of non-glucose-fermenting gram-negative bacilli.we evaluated vitek 2 cards (ngnc and ast-gn10) for the accuracy of identification (id) and antimicrobial susceptibility testing (ast) of non-glucose-fermenting gram-negative bacilli (ngf-gnb). in a total of 201 strains, 190 strains (94.5%) were correctly identified, seven strains (3.5%)showed low discrimination, four strains (2.0%) had discrepancies, and no strain remained unidentified.reference ast of amikacin, aztreonam, cefepime, cefotaxime, ceftazidime, ciprofloxacin, imipenem,levofloxacin, ...200919343822
bacterial communities in a crude oil gathering and transferring system (china).bacterial communities in crude oil and oil field production water samples from an oil gathering and transferring system in changqing oil field in china were investigated by 16s rrna denaturing gradient gel electrophoresis (dgge) analysis followed by gene cloning and sequencing. dgge profiles showed that bacterial communities are far more rich in the water samples than that in the crude oil samples, and that bacteria related to ochrobactrum sp. and stenotrophomonas sp. were detected in all crude ...200919351561
pseudomonas aeruginosa las quorum sensing autoinducer suppresses growth and biofilm production in legionella species.bacteria commonly communicate with each other by a cell-to-cell signalling mechanism known as quorum sensing (qs). recent studies have shown that the las qs autoinducer n-(3-oxododecanoyl)-l-homoserine lactone (3-oxo-c(12)-hsl) of pseudomonas aeruginosa performs a variety of functions not only in intraspecies communication, but also in interspecies and interkingdom interactions. in this study, we report the effects of pseudomonas 3-oxo-c(12)-hsl on the growth and suppression of virulence factors ...200919383702
stenotrophomonas maltophilia infections of intact skin: a systematic review of the literature.to highlight the importance of intact skin infection syndromes caused by stenotrophomonas maltophilia, we review 17 reported cases. skin infection syndrome presentations included metastatic cellulitis (58%), primary cellulitis (23%), and ecthyma gangrenosum (17%). associated risk factors were hematologic malignancies and chemotherapy (94%), neutropenia (94%), presence of central venous catheter (17%), and exposure to broad-spectrum antibiotics (84%). the diagnosis was supported by cultures of sk ...200919070451
stenotrophomonas maltophilia: an emerging opportunist human pathogen.stenotrophomonas maltophilia has emerged as an important opportunistic pathogen in the debilitated host. s maltophilia is not an inherently virulent pathogen, but its ability to colonise respiratory-tract epithelial cells and surfaces of medical devices makes it a ready coloniser of hospitalised patients. s maltophilia can cause blood-stream infections and pneumonia with considerable morbidity in immunosuppressed patients. management of infection is hampered by high-level intrinsic resistance to ...200919393961
volatile organic compounds: a potential direct long-distance mechanism for antagonistic action of fusarium oxysporum strain msa 35.fusarium oxysporum msa35 [wild-type (wt) strain] is an antagonistic fusarium that lives in association with a consortium of bacteria belonging to the genera serratia, achromobacter, bacillus and stenotrophomonas in an italian soil suppressive to fusarium wilt. typing experiments and virulence tests provided evidence that the f. oxysporum isolate when cured of the bacterial symbionts [the cured (cu) form], is pathogenic, causing wilt symptoms identical to those caused by f. oxysporum f. sp. lactu ...200919396945
[comparison between agar dilution and three other methods for determining the susceptibility of 228 clinical isolates of non-fermenting gram-negative rods].susceptibility testing for non-fermenting gram-negative rods (nfgnr) is problematic; valid methods are needed for this purpose.200919406523
analysis of community structure of a microbial consortium capable of degrading benzo(a)pyrene by dgge.a microbial consortium was obtained by enrichment culture of sea water samples collected from botan oil port in xiamen, china, using the persistent high concentration of a mixture of polycyclic aromatic hydrocarbons enrichment strategy. denaturing gradient gel electrophoresis (dgge) was used to investigate the bacterial composition and community dynamic changes based on pcr amplification of 16s rrna genes during batch culture enrichment. using the spray-plate method, three bacteria, designated a ...200919409577
ampdi is involved in expression of the chromosomal l1 and l2 beta-lactamases of stenotrophomonas maltophilia.two ampd homologues, ampd(i) and ampd(ii), of stenotrophomonas maltophilia have been cloned and analyzed. comparative genomic analysis revealed that the genomic context of the ampd(ii) genes is quite different, whereas that of the ampd(i) genes is more conserved in s. maltophilia strains. the ampd system of s. maltophilia is distinct from that of the enterobacteriaceae and pseudomonas aeruginosa in three respects. (i) ampd(i) of s. maltophilia is not encoded in an ampde operon, in contrast to wh ...200919414581
the role of ampr in regulation of l1 and l2 beta-lactamases in stenotrophomonas maltophilia.stenotrophomonas maltophilia is known to produce at least two chromosomal-mediated inducible beta-lactamases, l1 and l2. gene l2, which encodes a class a beta-lactamase, and the adjacent ampr gene form an ampr-class a beta-lactamase module. l1 belongs to the class b beta-lactamase and has no neighbor ampr-like regulatory gene. in this study, the ampr-l2 module from s. maltophilia kh was compared with ampr-beta-lactamase modules from several microorganisms with respect to the ampr and beta-lactam ...200919071216
emerging pathogens in cystic fibrosis: ten years of follow-up in a cohort of patients.in patients with cystic fibrosis (cf), there is an increasing incidence of some uncommon respiratory pathogens, such as burkholderia cepacia complex (bcc), stenotrophomonas maltophilia, and achromobacter xylosoxidans. in order to evaluate the prevalence and the clinical impact of these pathogens, we retrospectively studied a total of 109 patients followed in our center from 1996 to 2006 and reviewed the results of 1,550 sputum samples. the isolation of pseudomonas aeruginosa slightly decreased o ...200918758832
fluorescence spectroscopy as a promising tool for a polyphasic approach to pseudomonad taxonomy.fluorescence spectroscopy is an emerging tool for the analysis of biomolecules from complex matrices. we explored the potentialities of the method for the pseudomonad taxonomic purpose at the genus and species level. emission spectra of three intrinsic fluorophores (namely, nadh, tryptophan, and the complex of aromatic amino acids and nucleic acid) were collected from whole bacterial cells. their comparisons were performed through principal component analysis and factorial discriminant analysis. ...200918815829
risa-hplc analysis of lung bacterial colonizers of cystic fibrosis children.microbiological analysis of sputum samples, from children affected by cystic fibrosis (cf) and showing signs of acute or chronic infections, is routinely performed by culture-dependent approaches involving selective media and biochemical tests. these identification schemes are time-consuming, and may lead to false negative results. the aim of this work was to evaluate the efficacy of a ribosomal intergenic spacer analysis (risa) coupled to high performance liquid chromatography (hplc) for the de ...200918929602
role of a moderately halophilic bacterial consortium in the biodegradation of polyaromatic hydrocarbons.polycyclic aromatic hydrocarbons are ubiquitous pollutants in the environment, and most high molecular weight pahs cause mutagenic, teratogenic and potentially carcinogenic effects. while several strains have been identified that degrade pahs, the present study is focused on the degradation of pahs in a marine environment by a moderately halophilic bacterial consortium. the bacterial consortium was isolated from a mixture of marine water samples collected from seven different sites in chennai, i ...200918995870
identification of novel denitrifying bacteria stenotrophomonas sp. zz15 and oceanimonas sp. yc13 and application for removal of nitrate from industrial wastewater.two novel denitrifying bacteria were successfully isolated from industrial wastewater and soil samples. using morphological, biochemical/biophysical and 16s rrna gene analyses, these two bacteria were identified as stenotrophomonas sp. zz15 and oceanimonas sp. yc13, respectively. both of these two bacteria showed efficient no(3) (-)-n removing abilities under a semi-anaerobic condition without obvious accumulation of no(2) (-)-n, n(2)o-n and nh(4) (+)-n. no(3) (-)-n removal from paper mill waste ...200919002594
growing old with cystic fibrosis - the characteristics of long-term survivors of cystic fibrosis.the proportion of patients with cystic fibrosis (cf) who are middle-aged is increasing - and is likely to continue to do so. we surveyed a population of long-term cf survivors to assess their burden of illness and profile their disease characteristics.200919022643
viable ultramicrocells in drinking water.to examine the diversity of cultivable 0.2 micron filtrate biofilm forming bacteria from drinking water systems.200919040704
genome survey and characterization of endophytic bacteria exhibiting a beneficial effect on growth and development of poplar trees.the association of endophytic bacteria with their plant hosts has a beneficial effect for many different plant species. our goal is to identify endophytic bacteria that improve the biomass production and the carbon sequestration potential of poplar trees (populus spp.) when grown in marginal soil and to gain an insight in the mechanisms underlying plant growth promotion. members of the gammaproteobacteria dominated a collection of 78 bacterial endophytes isolated from poplar and willow trees. as ...200919060168
in vitro activities of a novel nanoemulsion against burkholderia and other multidrug-resistant cystic fibrosis-associated bacterial species.respiratory tract infection, most often involving opportunistic bacterial species with broad-spectrum antibiotic resistance, is the primary cause of death in persons with cystic fibrosis (cf). species within the burkholderia cepacia complex are especially problematic in this patient population. we investigated a novel surfactant-stabilized oil-in-water nanoemulsion (nb-401) for activity against 150 bacterial isolates recovered primarily from cf respiratory tract specimens. these specimens includ ...200918955531
morphological and genetic analysis of three bacteriophages of serratia marcescens isolated from environmental water.increases in multidrug-resistant strains of serratia marcescens are of great concern in pediatrics, especially in neonatal intensive care units. in the search for bacteriophages to control infectious diseases caused by multidrug-resistant s. marcescens, three phages (ksp20, ksp90, and ksp100) were isolated from environmental water and were characterized morphologically and genetically. ksp20 and ksp90 belonged to morphotype a1 of the family myoviridae, and ksp100 belonged to morphotype c3 of the ...200919087204
acquisition and spread of acinetobacter baumannii and stenotrophomonas maltophilia in intensive care patients.acinetobacter baumannii and stenotrophomonas maltophilia are increasingly important pathogens, especially in the intensive care units (icus). this study was designed to investigate the clonality, the mode of transmission and the patients' risk profile for acquisition of a. baumannii and s. maltophilia at the icu of an italian hospital. patterns of a. baumannii and s. maltophilia acquisition in the icu during the period of the survey were carriage, colonization and infection. characterization of ...200918771951
genes involved in arsenic transformation and resistance associated with different levels of arsenic-contaminated soils.arsenic is known as a toxic metalloid, which primarily exists in inorganic form [as(iii) and as(v)] and can be transformed by microbial redox processes in the natural environment. as(iii) is much more toxic and mobile than as(v), hence microbial arsenic redox transformation has a major impact on arsenic toxicity and mobility which can greatly influence the human health. our main purpose was to investigate the distribution and diversity of microbial arsenite-resistant species in three different a ...200919128515
application of compochip microarray to investigate the bacterial communities of different composts.a microarray spotted with 369 different 16s rrna gene probes specific to microorganisms involved in the degradation process of organic waste during composting was developed. the microarray was tested with pure cultures, and of the 30,258 individual probe-target hybridization reactions performed, there were only 188 false positive (0.62%) and 22 false negative signals (0.07%). labeled target dna was prepared by polymerase chain reaction amplification of 16s rrna genes using a cy5-labeled universa ...200918818861
cellular and biochemical response to cr(vi) in stenotrophomonas sp.chromium (cr)-resistant bacteria isolated from a soil with 6 g kg(-1) of cr were identified based on 16s rrna gene sequence analysis as a stenotrophomonas, and designated as jd1. growth of jd1 was accompanied by transformation of cr(vi) to cr(iii) in liquid medium initially containing 300 mg l(-1) cr(vi), the maximum concentration allowing growth. jd1 produced the highest levels of a cr(vi)-binding exopolysaccharide when grown in medium with 100 mg l(-1) cr(vi). the relative exopolysaccharide mo ...200919146572
stenotrophomonas maltophilia induced post-cataract-surgery endophthalmitis: outbreak investigation and clinical courses of 26 patients.stenotrophomonas maltophilia, a microorganism which colonizes plastic material, is a rare causative agent of iatrogenic endophthalmitis.200919148575
production of oligosaccharide from alginate using pseudoalteromonas agarovorans.a marine bacterium was isolated from seawater near the korean south coast for efficient saccharification from alginate. based on 16s rdna sequence, the isolated strain was identified as pseudoalteromonas agarovorans. various environmental factors affecting saccharification of alginate using p. agarovorans cho-12 have been investigated in flask cultures. the optimum concentration of sugar was obtained at 30 rpm and 29 degrees c. among various nacl concentrations, when nacl concentration was incre ...200919160071
tigecycline in combination with other antimicrobials: a review of in vitro, animal and case report studies.tigecycline has been investigated in combination with other antibacterials against a wide range of susceptible and multiresistant gram-positive and gram-negative bacteria. combinations have been analysed in vitro, in animal models and in human case reports. in vitro, tigecycline combined with other antimicrobials produces primarily an indifferent response (neither synergy nor antagonism). nevertheless, synergy occurred when tigecycline was combined with rifampicin against 64-100% of enterococcus ...200919162449
changes in bacterial isolates from burn wounds and their antibiograms: a 20-year study (1986-2005).our aim is to elucidate shifts in the bacterial spectrum colonising burn wounds and corresponding antibiotic susceptibilities during a 20-year study period.200919167827
characterization of the charge variants of l2 beta-lactamase in stenotrophomonas maltophilia.stenotrophomonas maltophilia kh has two acid beta-lactamases with isoelectric points (pis) of 4.6 and 5.4, and several basic beta-lactamases (pis >7.0) that produce a ladder-shaped pattern by ief. an isogenic l2 mutant, khl2(xyle), was constructed by gene replacement. from ief and native page zymograms of strains kh and khl2(xyle), it was demonstrated that the basic beta-lactamases and the acid beta-lactamase with pi 5.4 are encoded by the same l2 gene and that the active types of these l2 charg ...200919208880
stenotrophomonas chelatiphaga sp. nov., a new aerobic edta-degrading bacterium.a new ethylenediaminetetraacetic acid (edta)-utilizing gammaproteobacterial strain lpm-5(t) was isolated from municipal sewage sludge. aerobic, gram-negative, motile rods multiply by binary fission. neutrophilic and mesophilic, these are unable to grow in the presence of 3% nacl (w/v), and unable to reduce nitrate to nitrite, and are oxidase and catalase positive, but lipase negative. the major cellular fatty acids are c(i15:0), c(a15:0) and c(16:1w7c). the dominant phospholipids are phosphatidy ...200919216044
community-acquired stenotrophomonas maltophilia infections: a systematic review.stenotrophomonas maltophilia is a pathogen that causes infections mainly in immunocompromised patients. however, community-acquired s. maltophilia infections have been occasionally reported. the objective of this paper was to collect and evaluate the available published data referring to community-acquired s. maltophilia infections. we searched pubmed, the cochrane library, and scopus for articles providing data for patients with community-acquired s. maltophilia infections. eight case series an ...200919224257
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