Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| acid alpha naphthyl acetate esterase reacting lymphocytes in the peripheral blood of chickens challenged with marek's disease after vaccination with three different vaccines. | the average percentage of acid alpha naphthyl acetate esterase reacting lymphocytes (aparl) was enumerated in the peripheral blood of chickens challenged with marek's disease after vaccination with either turkey herpesvirus (hvt), inactivated marek's disease virus (imdv) or a mixture of the two (bivalent vaccine). a gradual increase in aparl value was noticed in the vaccinated chickens from day 7 to 70 after challenge with a virulent marek's disease virus. the increase was consistent and signifi ... | 1987 | 3628992 |
| restriction enzyme map of herpesvirus of turkey dna and its collinear relationship with marek's disease virus dna. | the genome of herpesvirus of turkey (hvt) was shown to consist of long and short unique regions flanked by inverted repeats (j. cebrian, kaschka-dietrich, c., berthelot, n., and sheldrick, p., 1982, proc. natl. acad. sci. usa 79, 555-558). in this paper we report the construction of the linkage map of hvt dna for bamhi, hindiii, and psti restriction endonucleases. the maps were constructed by hybridization of 19 cloned bamhi fragments of hvt dna to electrophoretically separated digests of genomi ... | 1987 | 3029976 |
| efficacy of a bivalent vaccine against marek's disease. | a bivalent vaccine was prepared by combining inactivated marek's disease virus and turkey herpesvirus. the efficacy of this vaccine, compared to turkey herpesvirus and inactivated marek's disease virus separately, was studied in unsexed white leghorn chicks which were vaccinated at one day old and then challenged at 21 days old with fowl blood infected with virulent marek's disease virus. the bivalent vaccine appreciably delayed mortality resulting from marek's disease and elicited the highest p ... | 1987 | 3035660 |
| effect of acyclovir on the replication of turkey herpesvirus and marek's disease virus. | the toxicity of acyclovir for chick embryo fibroblasts and its effect on the replication of turkey herpesvirus (strain fc 126) and marek's disease virus (strain hprs 16) multiplied on fibroblast culture was studied. the influence of using acyclovir on the development of the tumour process in birds infected with a virulent marek's disease virus was also determined. acyclovir used in doses below 12.5 micrograms ml-1 proved to be nontoxic for chick embryo fibroblast culture. it inhibited in vitro r ... | 1987 | 3039621 |
| marek's disease: a model for protection against herpesvirus-induced tumours. | marek's disease (md) is a lymphoproliferative disease of chickens caused by a herpesvirus (mdv). several effective vaccines have been developed and md is therefore often considered as a model for studying antitumour vaccines. key factors for the understanding of vaccine-induced immunity are discussed. three serotypes have been characterized: serotype 1 or oncogenic mdv, serotype 2 or non-oncogenic mdv and serotype 3 or herpesvirus of turkeys. the three serotypes have clearly different genomes an ... | 1987 | 2825984 |
| isolation of chicken anaemia agent and marek's disease virus from chickens vaccinated with turkey herpesvirus and lesions induced in chicks by inoculating both agents. | a disease that was characterised by high mortality, necrotic lesions, severe thymic and bursal atrophy, as well as marek's disease (md) lesions, occurred in two young layer chicken flocks vaccinated with turkey herpesvirus (hvt) at hatching. an agent similar to the chicken anaemia agent, designated caa82-2, and a virulent md virus strain, designated md82-2, were isolated from the kidneys of affected chickens. dual inoculation of young chicks with both agents resulted in early mortality associate ... | 1987 | 18766615 |
| enhancement of natural killer cell activity by marek's disease vaccines. | vaccination against marek's disease with herpesvirus of turkeys (hvt) has been reported to cause increased natural killer (nk) cell activity as detected in vitro against lscc-rp9 target cells. the effect of vaccination with sb-1 (a nononcogenic chicken herpesvirus), hvt and the hvt/sb-1 combination on nk cell activation was compared in marek's disease susceptible (p-2) and resistant (n-2) chickens. birds were vaccinated at 7 days of age and nk cell activity was measured between 3 and 42 days aft ... | 1987 | 18766591 |
| embryo vaccination of chickens with turkey herpesvirus: characteristics of the target cell of early viral replication in embryonic lung. | inoculation of turkey herpesvirus (hvt) into chicken eggs at the 17th day of embryonation resulted in recovery of high titres of the virus from the lung of the embryo. infection of the lung was not characterised by extensive virus-induced cell lysis because in situ viral internal antigen (via) of hvt was not detected. examination of single cell suspensions of lungs from embryos at peak viral activity revealed that the target cells of virus infection were adherent and lacked surface antigens dete ... | 1987 | 18766646 |
| herpesviruses provide helper functions for avian adeno-associated parvovirus. | the avian herpesviruses infectious laryngotracheitis virus (iltv) and herpesvirus of turkeys (hvt), as well as the mammalian herpesvirus pseudorabies virus (prv) were able to provide complete helper activity for the production of infectious avian adeno-associated virus (aaav) in chicken cells. the presence of aaav in the infected chicken cell reduced the multiplication of hvt. iltv or prv, however, were not affected if used as helper viruses. infectious aaav was determined by an indirect immunof ... | 1986 | 3003233 |
| similarities and dissimilarities in the structure and expression of viral genomes of various virus strains immunologically related to marek's disease virus. | various strains immunologically related to marek's disease virus (mdv) have been subdivided into three serotypes: serotype 1, pathogenic strains of mdv and attenuated or apathogenic variants derived from them; serotype 2, naturally occurring apathogenic strains of mdv; serotype 3, herpesvirus of turkey (hvt). the viral genome structures of these three serotypes were compared by a simple, practical method using total dna extracted from virus-infected cells instead of viral dna purified from virio ... | 1986 | 2424404 |
| monoclonal antibody to chicken fetal antigen on marek's disease lymphoblastoid cell line mdcc-msb1. | a monoclonal antibody (2h3) to chicken fetal antigen (cfa) expressed on the cell surface of the marek's disease lymphoblastoid cell line mdcc-msb1 was generated. 2h3 reacted specifically with various cells from chicken embryos, especially with fetal red blood cells, fetal bursa-derived lymphocytes, fetal liver cells, and embryo fibroblasts but not with fetal peripheral blood lymphocytes. 2h3 reacted also with all lymphoblastoid cell line cells tested: marek's disease, avian leukosis, and reticul ... | 1986 | 2428485 |
| pathogenicity studies with plaque-purified preparations of marek's disease virus strain cvi-988. | the pathogenicity of marek's disease (md) strain cvi-988 vaccine, eight plaque-purified preparations originating from this strain, and the vaccine hvt fc126 (based on herpesvirus of turkeys) was determined by intramuscular administration of high virus doses to day-old specific-pathogen-free rhode island red (rir) chickens, which are extremely md-susceptible. paralysis and neuritis were observed in 88% of rir chickens inoculated with mdv cvi-988 at the cell-passage level of the commercial vaccine ... | 1986 | 3015112 |
| characterization of herpesvirus of turkeys. replication using recombinant dna. | dna has been isolated from herpesvirus of turkeys (hvt) virions and used to construct a partial gene library in pbr-322. the recombinants have been characterized and shown to contain hvt dna inserts. a representative recombinant containing a 5.9-kilobase hindiii fragment was used as a probe to quantitate the yields of hvt dna in vitro and to follow the kinetics of viral dna replication. the data shown that in chicken fibroblasts, viral dna synthesis initiates by about 12-14 hr postinfection and ... | 1986 | 3729874 |
| virus-induced atherosclerosis. herpesvirus infection alters aortic cholesterol metabolism and accumulation. | infection of normocholesterolemic, specific-pathogen-free chickens with marek's disease herpesvirus (mdv) has been shown histologically to lead to chronic atherosclerosis like that in humans. the development of herpesvirus-induced atherosclerosis in vivo and the presence of specific marek's antigen within aortic cells suggested that mdv infection may modify lipid metabolism and lead to significant lipid accumulation. experiments reported herein were designed to determine the types and quantity o ... | 1986 | 2934987 |
| chicken embryonal vaccination with avian infectious bronchitis virus. | a commercial infectious bronchitis virus (ibv) vaccine of the massachusetts 41 strain was injected in embryonating chicken eggs on embryonation day (ed) 18. the ibv vaccine was pathogenic for embryos, and it was passaged in chicken kidney tissue culture to reduce the pathogenicity. at the 40th tissue culture passage (p40-ibv), the virus became apathogenic for the embryos. maternal antibody-positive or -negative chicks hatching from eggs injected with p40-ibv developed antibody to ibv and were pr ... | 1986 | 3008613 |
| herpesvirus infection prevents activation of cytoplasmic cholesteryl esterase in arterial smooth muscle cells. | herpesvirus infection has been shown to alter the cholesteryl ester cycle in avian arterial smooth muscle cells, resulting in cytoplasmic cholesteryl ester accumulation (hajjar, d. p., falcone, d. j., fabricant, c. g., and fabricant, j. (1985) j. biol. chem. 260, 6124-6128). in this study, we attempted to define some of the regulatory mechanisms associated with the control of cytoplasmic cholesteryl esterase in marek's disease herpesvirus (mdv)-infected cells. we found that cholesteryl esterase ... | 1986 | 3011790 |
| use of lectins to detect and differentiate subtypes of marek's disease virus and turkey herpesvirus glycoproteins in tissue culture. | biotinylated lectins in conjunction with an avidin-biotin-peroxidase complex were used for the first time to reveal glycoproteins in chicken and duck embryo fibroblasts infected with three prototype members of the avian herpesvirus group, marek's disease virus serotypes 1 and 2 and turkey herpesvirus. by using a panel of 10 lectins, a pattern of reactivity emerged which was both group- and type-specific. morphological details of the lectin-stained cells include cytoplasmic granulation, capping a ... | 1986 | 3016013 |
| a comparison of the biological properties of type 2 plaque-producing agent derived from the cal-1 strain of marek's disease virus with other related viruses. | the serological and biological properties of the type 2 plaque-producing agent (ppa) derived from the cal-1 strain of marek's disease virus (mdv) were compared with those of reference strains of the three serotypes of mdv and herpesvirus of turkeys (hvt) groups; namely jm, hprs-24 strains of mdv and the fc-126 strain of hvt for serotype 1, 2, and 3, respectively. by agar gel precipitation (agp), indirect fluorescent antibody and virus neutralization tests, type 2 ppa was related but not identica ... | 1986 | 3013131 |
| protective efficacy of marek's disease virus (mdv) cvi-988 cef65 clone c against challenge infection with three very virulent mdv strains. | comparative 50% protective dose (pd50) assays were performed using a plaque-purified preparation of marek's disease virus (mdv) strain cvi-988 at the 65th chicken embryo fibroblast (cef) passage level (mdv cvi-988 cef65 clone c) and three commercial md vaccines: herpesvirus of turkeys (hvt) fc126, mdv cvi-988 cef35, and a bivalent vaccine composed of hvt fc126 and mdv sb-1. in addition, comparative pd50 assays were performed in groups of chickens with maternal antibody to each of the three vacci ... | 1986 | 3015113 |
| chronological observations of marek's disease-associated feather-pulp lesions in field chickens. | fourteen test groups consisting of a total of 541 field chickens in six rearing farms, all of which were vaccinated with turkey herpesvirus at one day old, were investigated for chronological changes in marek's disease (md)-associated feather-pulp lesions (fpl) and their association with nuclear-inclusion (ni) formation in the feather-follicle epithelium (ffe) and with incidence of md. the fpl observed were categorized into r1-type (non-tumorous lymphoid lesion), r2-type (inflammatory lesion con ... | 1986 | 3015114 |
| molecular characterization of marek's disease herpesvirus b antigen. | the marek's disease herpesvirus (mdhv) b antigen (mdhv-b) was identified and molecularly characterized as a set of three glycoproteins of 100,000, 60,000, and 49,000 apparent molecular weight (gp100, gp60, and gp49, respectively) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) after immunoprecipitation from [35s]methionine-labeled infected cells by specific rabbit antiserum directed against mdhv-b (r alpha b), as previously determined by immunodiffusion. further identific ... | 1986 | 3016305 |
| isolation of very virulent pathotypes of marek's disease virus from vaccinated chickens in europe. | two very virulent strains of marek's disease virus were isolated from two separate farms in northern italy that had experienced outbreaks of marek's disease in vaccinated flocks. the isolates were similar to very virulent strains reported in the usa in terms of their enhanced pathogenicity, both for chickens vaccinated with the herpesvirus of turkeys, and for genetically resistant chickens. this first description of very virulent strains of marek's disease virus from outside the usa suggests tha ... | 1986 | 3016970 |
| preparation of monoclonal antibodies against marek's disease virus and herpesvirus of turkeys. | 1986 | 3029494 | |
| establishment of a variant type of turkey herpesvirus which releases cell-free virus into the culture medium in large quantities. brief report. | a variant turkey herpesvirus (hvt/vt) which releases 10(5.0) plaque-forming units per 0.1 ml of cell-free virus into the culture medium was established from the prototype hvt fc 126 strain (hvt/wt). many enveloped and naked virions of hvt/vt but only naked virions of hvt/wt were found in the culture medium. by the hindiii restriction cleavage patterns, the hvt/vt dnas were almost identical to those of hvt/wt. the hvt/vt was replication-defective in chickens. | 1986 | 3753201 |
| a study of marek's disease in japanese quails vaccinated with herpesvirus of turkeys. | in a study of outbreaks of marek's disease in quails, 220 adult quails vaccinated with herpesvirus of turkeys (hvt) were examined pathologically and serologically for marek's disease (md). forty-three of the 220 quails exhibited microscopic lesions similar to those of chickens with md. md-virus-specific antigen was found in feather tips of 44 of the 220 birds, and the hvt-specific antibody was found in sera of 56 of the 220 birds by agar gel precipitation. there was a positive correlation betwee ... | 1986 | 3814019 |
| partial protection against marek's disease in chickens immunized with glycoproteins gb purified from turkey-herpesvirus-infected cells by affinity chromatography coupled with monoclonal antibodies. | glycoproteins gb of marek's disease virus (mdv) and herpesvirus of turkeys (hvt) related to virus neutralization were purified from hvt-infected cells by affinity chromatography. immunization of chickens with purified glycoproteins gb resulted in partial protection against md. neutralizing antibodies were detected in chickens immunized with hvt-gb. | 1985 | 3896223 |
| field trials to test the efficacy of polyvalent marek's disease vaccines in layer and broiler breeder chickens. | as a follow-up to earlier trials to evaluate the efficacy of polyvalent marek's disease (md) vaccines in broilers, four trials were conducted with layer or broiler breeder flocks. chickens caccinated with trivalent (md11/75c plus sb-1 plus hvt) and bivalent (sb-1 plus hvt) vaccines were compared with those vaccinated with turkey herpesvirus (hvt) alone. data from three of the four trials indicated polyvalent vaccines were more efficacious than hvt. however, critical interpretations were confound ... | 1985 | 3912753 |
| inhibition of turkey herpesvirus replication by anti-cellular serum. | quail embryo fibroblasts were used to investigate how rabbit and chicken antisera against chicken erythrocytes carrying different b alleles of the major histocompatibility antigens affect the neutralization of herpesvirus of turkeys (hvt). although the neutralizing activities of these antisera were rather low, the hvt propagated in chicken embryo fibroblasts (cef) from certain genotypic embryos was neutralized more by the antisera to the corresponding erythrocytes. after absorption of these anti ... | 1985 | 3985879 |
| altered cholesteryl ester cycle is associated with lipid accumulation in herpesvirus-infected arterial smooth muscle cells. | we describe herein the effects of marek's disease herpesvirus (mdv) on cholesterol and cholesteryl ester metabolism in cultured chicken arterial smooth muscle cells. infection of arterial smooth muscle cells from specific pathogen-free chickens with mdv, but not a virus control, herpesvirus of turkeys led to a 7-10-fold increase in the accumulation of free and esterified cholesterol and a 2-fold increase in phospholipids. the cellular lipid changes observed in the mdv-infected arterial smooth mu ... | 1985 | 3997816 |
| monoclonal antibodies against virus-specific antigens in culture cells infected with herpesvirus of turkeys. | 1985 | 4068446 | |
| gel electrophoretic analysis of polypeptides from nucleocapsids of marek's disease virus strains and herpesvirus of turkey. | the polypeptides of nucleocapsids of marek's disease virus (mdv) strains with different biological properties and of antigenically related herpesvirus of turkey (hvt) strains were analysed by one- and two-dimensional (1d and 2d, respectively) gel electrophoresis. based on small differences in migration behaviour (size and charge) of a number of corresponding nucleocapsid polypeptides, the virus strains could be differentiated into three groups. the polypeptide pattern of group i, comprising the ... | 1985 | 2986577 |
| differences in the processing of secreted glycoprotein a induced by marek's disease virus and herpesvirus of turkeys. | the steps from the precursor to the processed forms of a secreted glycoprotein (ga) of marek's disease virus (mdv) and of herpesvirus of turkeys (hvt) were examined by two-dimensional gel electrophoresis of immunoprecipitates from the cell lysate and medium of infected cultures with monoclonal antibodies. differences between mdv- and hvt-ga were particularly observed in isoelectric points of the glycosylated or unglycosylated precursor forms. | 1985 | 2987399 |
| vaccination against marek's disease and infectious bursal disease iii. growth rate of both turkey herpesvirus and infectious bursal disease virus in coinfected cell cultures. | titers of the turkey herpesvirus (hvt) and infectious bursal disease virus (ibdv) in coinfected cell cultures, used to produce a live bivalent hvt/ibdv vaccine, were determined daily. based on the daily titers, cells harvested either 2 or 3 days after inoculating the second virus (ibdv) into hvt infected cell cultures yielded the maximum titers for both viruses. harvesting coinfected cell cultures on either day provided the most suitable source of the live hvt/ibdv vaccine against marek's diseas ... | 1985 | 2987896 |
| production of herpesvirus of turkeys in microcarrier culturing system--a new method for production of vaccine against marek's disease. | a microcarrier (mc) culturing system for production of a vaccine against marek's disease virus is presented. cytodex-3 beads (pharmacia) and de-53 microgranular (whatman) are the recommended microcarriers. primary cells from chick embryo as well as cells from a quail cell line (qt-6, established from a japanese quail) were found to be good hosts for growth of herpesvirus of turkeys (hvt). hvt, the vaccine strain against marek's disease, was successfully grown and harvested on the above mentioned ... | 1985 | 2995176 |
| map location of homologous regions between marek's disease virus and herpesvirus of turkey and the absence of detectable homology in the putative tumor-inducing gene. | the dna region having homology between marek's disease virus and herpesvirus of turkey was assigned to the restriction map of marek's disease virus by southern blot hybridization. under moderate conditions at the level of 15% mismatching, homology was found to be distributed throughout the marek's disease virus genome. the long inverted-repeat regions (trl and irl), which are considered to play a significant role in tumorigenicity, did not show any homology to herpesvirus of turkey dna. | 1985 | 2983105 |
| simultaneous application of live turkey herpesvirus and infectious bursal disease vaccines against marek's disease and infectious bursal disease. | the efficacy of cell-associated (ca) and cell-free (cf) infectious bursal disease (ibd) vaccines in maternal antibody (mab)-bearing broiler chickens was compared. compatibility and potency of a mixed vaccine combining live ca turkey herpesvirus (hvt) and ca ibd vaccine viruses were also tested against gross lesions induced by marek's disease (md) or ibd in specific-pathogen-free chickens. both ca and cf ibd vaccines provided significant protection against gross bursal lesions of ibd in the prese ... | 1985 | 2983300 |
| evaluation of dna homology of marek's disease virus, herpesvirus of turkeys and epstein-barr virus under varied stringent hybridization conditions. | marek's disease virus (mdv) showed only 0.3-0.6% homology in dna sequence with herpesvirus of turkeys (hvt) at tm-24.4 degrees c in spite of its antigenic similarity to the latter. southern blot hybridization under stringent conditions showed that the homology between mdv and hvt is located in the restricted portion of these viral genomes. at tm-49.6 degrees c, which permits the detection of homology with one base mismatch in three between the mdv and hvt dnas, sequences with weak homology were ... | 1984 | 6086596 |
| studies on the serological cross-reaction between marek's disease virus and herpesvirus of turkeys using monoclonal antibodies to major virus-specific polypeptides. brief report. | a major virus-specific polypeptide antigenically cross-reactive between marek's disease virus (mdv) and herpesvirus of turkeys (hvt) was characterized by two monoclonal antibodies against mdv. the antigenic determinant recognized by one monoclonal antibody was present only in the mdv polypeptide but not in the hvt polypeptide, whereas the antigenic determinant recognized by the other antibody was present in both mdv and hvt polypeptides. peptide mapping showed the difference in the amino acid se ... | 1984 | 6206826 |
| comparative viral, immunologic, and pathologic responses of chickens inoculated with herpesvirus of turkeys as embryos or at hatch. | chickens were vaccinated with the herpesvirus of turkeys (hvt) at embryonation day 17 or 18 or at hatch and various responses of the 2 vaccinated groups were compared. in embryo-vaccinated chickens, hvt titers were high in the lungs before hvt could be isolated from other tissues. seemingly, embryos acquired infection via the respiratory tract. in hatch-vaccinated chickens, hvt was first isolated from spleen and then from other tissues. titers of recoverable hvt in tissues of embryo-vaccinated c ... | 1984 | 6089624 |
| field trials to test the efficacy of polyvalent marek's disease vaccines in broilers. | the efficacies of trivalent (md11/75c + sb-1 + hvt), bivalent (sb-1 + hvt), and turkey herpesvirus (hvt) vaccines against marek's disease (md) were compared in commercial broiler flocks in four trials involving 11 farm locations and 486,300 chickens. in all four trials, chickens receiving polyvalent vaccines had lower leukosis (md) condemnation rates than chickens vaccinated with hvt alone; when data were summarized for each vaccine type in each trial, condemnation rates for the bivalent- or tri ... | 1984 | 6326743 |
| extensive homology exists between marek disease herpesvirus and its vaccine virus, herpesvirus of turkeys. | marek disease is a lymphomatous disease of chickens caused by infection of a herpesvirus, marek disease virus (mdv). marek disease is the only neoplastic disease for which a successful vaccine has been developed. the vaccine virus, herpesvirus of turkeys (hvt), is non-oncogenic in chickens. despite the strong antigenic relationship between these viruses, previous studies showed that the two viral dnas share little or no homology. using less stringent hybridization conditions and methods that gre ... | 1984 | 6328512 |
| immunization against marek's disease transplantable cell lines. | continuous passage of mdcc-rp1, a highly tumorigenic marek's disease (md) lymphoblastoid cell line, in cell culture resulted in a gradual loss in ability of the cell line to cause progressive tumors in susceptible day-old chicks. inoculation of day-old chicks with high-cell-culture-passaged (187th to 417th) nontumorigenic mdcc-rp1 cells gave excellent protection against challenge at 8 days with low-passaged tumorigenic mdcc-rp1 cells but failed to protect against primary tumors caused by inocula ... | 1984 | 6326737 |
| identification with monoclonal antibodies of glycoproteins of marek's disease virus and herpesvirus of turkeys related to virus neutralization. | by use of monoclonal antibodies cross-reactive with marek's disease virus and herpesvirus of turkeys, three glycoproteins (for marek's disease virus, gp115/110, gp63, and gp50; for herpesvirus of turkeys, gp115, gp62 and gp52) related to virus neutralization were identified. immunization of chickens or rabbits with these glycoproteins purified by affinity chromatography resulted in production of neutralizing antibodies. | 1984 | 6321752 |
| latent herpesvirus infection of testes and spinal ganglia of turkeys with semen abnormalities. | the present study confirms that herpesviruses (lf) previously isolated from testes and buffy coat cells of male turkeys with semen abnormalities establish a latent infection in testicular cells. these experiments also present the first evidence that these herpesviruses are harbored latently in cells of spinal ganglia. one-week-old turkeys were inoculated with either the lf isolates or the prototype turkey herpesvirus fc 126; allowed to mature sexually through one breeding season and necropsied a ... | 1984 | 6095790 |
| processing of glycoprotein gb related to neutralization of marek's disease virus and herpesvirus of turkeys. | the glycoprotein gb related to neutralization of marek's disease virus (mdv) and herpesvirus of turkeys (hvt) is composed of several glycosylated polypeptides, which were immunoprecipitated with monoclonal antibodies and rabbit antiserum cross-reactive to mdv-gb and hvt-gb, and analyzed by sds-polyacrylamide gel electrophoresis. the present pulse-chase experiments showed that the precursor forms of mdv- and hvt-gb were glycoproteins with molecular weights of 110k to 115k (gp115/110) and 115k (gp ... | 1984 | 6094991 |
| vaccination against marek's disease and infectious bursal disease. ii. titration and in vivo efficacy of the coinfection-derived bivalent live vaccine for turkey herpesvirus and infectious bursal disease virus. | the bivalent hvt/ibdv live vaccine, developed by cocultivation of the turkey herpesvirus (hvt) and infectious bursal disease virus (ibdv) vaccine in cell cultures, was quantitated. the titer of each constituent virus could be successfully determined in vitro by pretreatment of the vaccine with anti-ibdv serum or chloroform (chcl3). studies in vivo further demonstrated that the anti-ibdv serum-treated hvt/ibdv vaccine lost its immunogenicity by failing to induce ibdv-neutralizing antibody or to p ... | 1984 | 6091083 |
| effect of infectious bursal disease virus on protection against marek's disease by turkey herpesvirus vaccine. | chickens inoculated at hatch with pathogenic infectious bursal disease virus (ibdv) and the turkey herpesvirus (hvt) vaccine were poorly protected against challenge at 1 week with virulent marek's disease virus (mdv). however, if vaccination with hvt preceded ibdv inoculation such as by embryonal vaccination with hvt and exposure to ibdv at hatch, or by vaccination at hatch and ibdv exposure 5 days or longer postvaccination, then the vaccinal efficacy of hvt was not influenced. further, the inhi ... | 1984 | 6091603 |
| monoclonal antibody-mediated immunoprecipitation of proteins from cells infected with marek's disease virus or turkey herpesvirus. | the major immunogenic viral proteins of marek's disease virus (mdv) and turkey herpesvirus (hvt) share antigenic determinants. the polyacrylamide gel electrophoresis pattern of five viral polypeptides immunoprecipitated with homologous convalescent chicken plasma was identical with the pattern obtained after immunoprecipitation with heterologous convalescent chicken plasma. a panel of monoclonal antibodies was used to identify mdv and hvt polypeptides. sixteen monoclonal antibodies were positive ... | 1984 | 6205501 |
| cell-specific antiviral activity of 1-(2-fluoro-2-deoxy-beta-d-arabinofuranosyl)-5-iodocytosine (fiac) against marek's disease herpesvirus and turkey herpesvirus. | three new fluoroarabinosylpyrimidine nucleosides (fiac, fiau and fmau) were tested for in vitro activity against oncogenic and nononcogenic strains of marek's disease virus (mdv) and herpesvirus of turkeys (hvt). marek's disease is a herpesvirus-induced lymphoma in chickens. nononcogenic strains of mdv and hvt can protect against this disease. all viruses were inhibited by 1 microm of these drugs in chick kidney cell (ckc) cultures, but only fmau and fiau were active in chicken embryo fibroblast ... | 1984 | 6097179 |
| the expression of marek's disease tumour-associated surface antigen in various avian species. | marek's disease tumour-associated surface antigen (matsa) was detected on lymphoid cells from chickens infected with marek's disease virus (mdv) or the herpesvirus of turkeys (hvt), from a chicken-quail hybrid infected with mdv, and from turkeys infected with the ga strain of mdv, but not on cells from turkeys infected with hvt or the hprs-16 strain of mdv or from mdv-infected japanese quails or bobwhite quails, despite the presence of lymphomas in some japanese quails. two complementary explana ... | 1984 | 18766850 |
| polyvalent marek's disease vaccines: safety, efficacy and protective synergism in chickens with maternal antibodies. | three marek's disease (md) vaccines were evaluated for safety and protective efficacy in chickens with maternal antibodies against serotype 1, 2 and 3 md viruses and in chickens with no maternal antibodies. the vaccines were: (1) md11/75c, an attenuated serotype 1 md virus, (2) a trivalent mixture of md virus strains md11/75c and sb-1, and turkey herpesvirus (hvt) strain fc 126, and (3) bivalent mixtures of these three viruses. these vaccines were compared with hvt or sb-1 vaccines in some trial ... | 1984 | 18766823 |
| effects of avian viruses on cultured chicken bone-marrow-derived macrophages. | cultured chicken bone-marrow-derived macrophages have been assayed for their susceptibility to infection with various avian viruses. three criteria of infection were employed: (1) virus-induced alterations in cell morphology ; (2) presence of intracellular viral antigens detectable by immunofluorescence; (3) kinetics of virus release by infected macrophages. macrophages proved to be resistant to marek's disease virus (mdv), herpesvirus of turkeys (hvt-fc126), infectious bronchitis virus (ibv) an ... | 1983 | 18766776 |
| in vivo and in vitro infections of chicken lymphocytes with marek's disease virus or herpesvirus of turkeys. | 1983 | 6323800 | |
| monoclonal antibodies reactive with the surface and secreted glycoproteins of marek's disease virus and herpesvirus of turkeys. | hybridomas were formed between mouse myeloma cells and spleen cells from mice immunized with marek's disease virus (mdv) or with herpesvirus of turkeys (hvt). three monoclonal antibodies were obtained, two (m26 and m34) from mdv clones and one (h9) from an hvt clone, all of which were specific for cross-reactive membrane antigen (ma) expressed on the surface of cells infected with mdv or hvt. all three antibodies also reacted with mdv- and hvt-specific glycoproteins in the molecular weight (mol. ... | 1983 | 6198439 |
| marek's disease herpesviruses. iv. molecular characterization of marek's disease herpesvirus a antigen. | marek's disease herpesvirus a antigen (mdhv-a) was identified as a 61,000- to 65,000-dalton glycoprotein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after immunoprecipitation from the culture medium of both [35s]methionine- and [14c]glucosamine-labeled infected cells by specific rabbit serum directed against mdhv-a. rigorous identification was accomplished by selective blocking of this specific immunoprecipitation of the glycoprotein with purified mdhv-a that was isolated at its ... | 1983 | 6300460 |
| monoclonal antibodies with specificity for three different serotypes of marek's disease viruses in chickens. | a total of 50 antibody-secreting hybridoma cells against marek's disease virus (mdv) and turkey herpesvirus (hvt) have been produced. eleven hybridomas were used for serotyping a panel of 15 pathogenic and nonpathogenic strains of mdv and hvt, representing three serotypes. the antibodies from the culture medium have fluorescence antibody (fa) titers of up to 100 and those from mouse ascitic fluid have titers ranging from 10(4) to 10(6). monoclonal antibody t81 is type-common, i.e., it reacts at ... | 1983 | 6184394 |
| comparative studies on antigens induced by turkey herpesvirus and marek's disease virus. i. agar gel precipitation and neutralization studies. | 1983 | 6196928 | |
| comparative studies on antigens induced by turkey herpesvirus and marek's disease virus. ii. immunofluorescent studies. | 1983 | 6197828 | |
| characteristics of marek's disease viruses isolated from vaccinated commercial chicken flocks: association of viral pathotype with lymphoma frequency. | fifty-three marek's disease (md) virus (mdv) isolates were obtained from turkey-herpesvirus-vaccinated broiler or layer flocks with excessive md losses, from control field flocks without excessive md losses, and from laboratory collections. twelve isolates were typed as very virulent (vvmdvs) on the basis of excessive pathogenicity for vaccinated chickens, 31 were typed as virulent (vmdvs), and 10 were typed as nonpathogenic (npmdvs). the npmdvs could be distinguished from turkey herpesviruses b ... | 1983 | 6303287 |
| most virus-specific polypeptides in cells productively infected with marek's disease virus or herpesvirus of turkeys possess cross-reactive determinants. | most virus-specific polypeptides in cells productively infected with marek's disease virus (mdv) or herpesvirus of turkeys (hvt) possess cross-reactive antigenic determinants, although the two viruses have very little dna homology. the cross-reactivity appeared to be more evident when [35s]methionine-labelled polypeptides were immunoprecipitated than when the [3h]glucosamine-labelled polypeptides were immunoprecipitated, suggesting that the glycoproteins of mdv and hvt may be less related in str ... | 1983 | 6187896 |
| embryo vaccination against marek's disease with serotypes 1, 2 and 3 vaccines administered singly or in combination. | marek's disease virus (mdv) vaccines of serotypes 1 and 2 administered in 18-day-old embryonated eggs induced better protection against post-hatch challenge at 3 days with virulent mdv than vaccines given at hatch. embryonal vaccination with a polyvalent vaccine containing equal quantities of serotypes 1 and 2 of mdv and serotype 3 virus (turkey herpesvirus, hvt) was also significantly more effective than post-hatch vaccination. these and earlier results indicate that protective efficacy of sing ... | 1983 | 6307248 |
| simultaneous vaccination of one-day-old broiler chickens with tenosynovitis virus and turkey herpesvirus. | an attenuated tenosynovitis virus vaccine and turkey herpesvirus (hvt) vaccine mixed prior to vaccination were injected subcutaneously into one-day-old chicks. in laboratory and field trials there did not appear to be any interference, because chickens vaccinated with both vaccines were effectively immunized against challenge with virulent marek's disease virus or tenosynovitis virus when compared with unvaccinated chickens or chickens vaccinated with either hvt or tenosynovitis virus. the combi ... | 1983 | 6312445 |
| field trials with a bivalent vaccine (hvt and sb-1) against marek's disease. | white leghorn chickens on five farms were given a bivalent marek's disease (md) vaccine consisting of turkey herpesvirus (hvt) and sb-1 (a nononcogenic md virus); other chickens received only hvt. the farms had histories of "vaccination failures," presumably owing to an exceptionally virulent challenge md virus. the bivalent vaccine uniformly protected chickens better than hvt alone between 12 and 16-20 weeks of age, when serious md losses occurred. during that period, total mortality in groups ... | 1983 | 6314982 |
| restriction endonuclease analysis of marek's disease virus dna and homology between strains. | the restriction endonuclease patterns of viral dna obtained from serotypes 1 and 2 strains of marek's disease virus have been compared and homology between the strains examined by hybridization. the results have shown that hprs 16 (serotype 1) dna has a structure similar to its attenuated variant hprs 16/att except for a few fragments that are present only in the virulent strain. evidence was obtained which suggested that these restriction fragments contained repeat sequences and that insertion ... | 1983 | 6319562 |
| vaccination against marek's disease and infectious bursal disease. i. development of a bivalent live vaccine by co-cultivating turkey herpesvirus and infectious bursal disease vaccine viruses in chicken embryo fibroblast monolayers. | vaccine viruses of marek's disease (md), the fc-126 strain of the herpesvirus of turkeys (hvt), and infectious bursal disease (ibd), the bursa-vac-m strain of ibd virus (ibdv), were propagated in the same chicken embryo fibroblast (cef) monolayers by superinfection. co-infection of the two viruses in the same cef culture or in a single cell can be demonstrated by staining with acridine orange and by electron microscopy.l this study was conducted with a superinfected live bivalent vaccine (hvt/ib ... | 1983 | 6322146 |
| herpesvirus-induced atherosclerosis in chickens. | repeated experiments have established that infection with marek's disease herpesvirus (mdv) leads to atherosclerosis in specific pathogen free (spf) normocholesterolemic chickens. neither normocholesterolemic nor hypercholesterolemic uninfected spf chickens develop this disease. the mdv-induced arterial disease is remarkably similar to chronic human atherosclerosis. cholesterol and saturated cholesteryl esters accumulated in cultured arterial smooth muscle cells (smc) infected with mdv. similar ... | 1983 | 6840298 |
| marek's disease virus and herpesvirus of turkeys: antigenic aspects. | 1983 | 6195671 | |
| antigenic relatedness of pigeon herpes encephalomyelitis virus to other avian herpesviruses. | pigeon herpes encephalomyelitis virus (phev) was compared with seven avian herpesviruses for antigenic relatedness using monospecific antisera and the indirect fluorescent-antibody (ifa), agar-gel-immunodiffusion, and serum-neutralization tests. no antigenic relationship was detected between phev and marek's disease virus, turkey herpesvirus, infectious laryngotracheitis virus, and duck enteritis virus. a common precipitating antigen was detected between the phev and pigeon herpesvirus (phv), ow ... | 1983 | 6196014 |
| establishment of b-lymphoblastoid cell lines from marek's disease virus-induced tumors in turkeys. | four lymphoblastoid cell lines were established from tumours of turkeys inoculated with high doses of the ga strain of marek's disease virus (mdv). unlike other md lymphoblastoid cell lines of chicken origin, these mdv transformed turkey line appear to be b lymphocytes and produce immunoglobulin. growth characteristics of these cell lines are slightly different; however, they all produce low levels of mdv-specific antigen and carry the complete genome of the virus as determined by virus rescue i ... | 1982 | 6277804 |
| marek's disease tumors in chickens vaccinated with turkey herpesvirus vaccine. | this study evaluated the protective ability of cell-free and cell-associated turkey herpesvirus (hvt) vaccine against marek's disease (md). four variables were evaluated: cell-free versus cell-associated hvt; dose of hvt; time of exposure to md virus (mdv), and comparison of md challenge virus types. it was determined that gross lesions of md were more prevalent in chickens placed in an environment at one day of age with three-week-old chickens shedding the cpf-1 isolate of md, as in chickens gi ... | 1982 | 7166202 |
| the early pathogenesis of turkey herpesvirus infection in chickens and turkeys. | the early responses of chickens and turkeys to infection with two strains of turkey herpesvirus (fc 126 and a recent field isolate, t-5a) were compared. all birds had similar virus rescue patterns from the spleen, regardless of virus strain used for infection. productive-restrictive infections as indicated by the presence of viral antigens were found only in the spleens of infected turkeys. viral antigens were not found in bursa or thymus but were found intermittently and at low levels in the sk ... | 1982 | 6285877 |
| the mechanism of genetic resistance to marek's disease in chickens. | genetic resistance to marek's disease in rpl line-6 chickens is expressed not only at the level of host immunological responses against virus an tumour antigens, but also at the level of target lymphoid cells for virus infection and transformation. the nature of the target cell involved was investigated. spleen cells from susceptible line-7 chickens adsorbed more marek's disease virus and turkey herpesvirus in vitro than line-6 spleen cells. in the case of marek's disease virus this was reflecte ... | 1982 | 6277808 |
| dose-dependent inhibition of virus rescue from lymphocytes latently infected with turkey herpesvirus or marek's disease virus. | the number of plaque-forming units (pfu) of turkey herpesvirus (hvt) isolated per 10(6) latently infected splenic lymphocytes was determined by co-cultivation on permissive monolayer cultures in 35-mm-diameter petri dishes. doses of 1 x 10(6) spleen cells or less per culture gave uniform dose-related titers, whereas doses of 8 x 10(6) cells often yielded less than 1-2% of the expected number of pfu. intermediate doses gave proportionally reduced virus yields. this dose-dependent inhibition was o ... | 1982 | 6285880 |
| influence of maternal antibody on efficacy of embryo vaccination with cell-associated and cell-free marek's disease vaccine. | vaccination with turkey herpesvirus (hvt) of 18-day-old chicken embryos from a commercial source or from a cross (15 x 7) of two inbred lines induced better protection against early post-hatch challenge with virulent marek's disease virus (mdv) than vaccination at hatch, despite the presence in embryos of maternally derived antibodies to hvt or to hvt and mdv. however, 50%-protective-dose (pd50) assays revealed that maternal antibodies in embryos reduced vaccine efficacy. the pd50 assays were co ... | 1982 | 6297450 |
| the epidemiology of avian herpesviruses in veterinary medicine. | there are ten avian herpesviruses, which have been isolated from eight orders. six of these are of veterinary importance: pacheco's parrot disease virus, pigeon herpesvirus, duck plague virus, infectious laryngotracheitis virus, herpesvirus of turkeys and marek's disease virus. the knowledge on the epidemiology of each virus and the disease it causes is discussed. features in common to infections with most avian herpesviruses are: infection is persistent in individuals and ubiquitous in populati ... | 1982 | 6299838 |
| characterization of non-oncogenic marek's disease virus-infected and turkey herpesvirus-infected lymphocytes. | 1982 | 6296281 | |
| inverted repeat nucleotide sequences in the genomes of marek disease virus and the herpesvirus of the turkey. | the dnas of two herpesvirus, the oncogenic marek disease virus and the serologically related herpesvirus of the turkey, were studied by electron microscopy. on the basis of fold-back molecules observed in single-stranded dna from both viruses, structures have been derived from the overall nucleotide sequence arrangement in their genomes. although differing in molecular weight, the genomes of marek disease virus and turkey herpesvirus are both constructed according to the same plan--two regions o ... | 1982 | 6281774 |
| resistance to marek's disease at hatching in chickens vaccinated as embryos with the turkey herpesvirus. | chickens vaccinated with herpesvirus of turkey (hvt) as 18-day embryos or at hatching were challenged as neonates with pathogenic marek's disease (md) virus (mdv). embryonally vaccinated chickens had much greater resistant to challenge than chickens vaccinated post-hatch. embryos became readily infected with hvt regardless of whether the vaccine was deposited into the body of the embryo or extraembryonally, such as in the amniotic sac. embryonally vaccinated chickens were viremic with hvt at hat ... | 1982 | 6284106 |
| marek's disease in turkeys: lack of protection by vaccination. | herpesvirus of turkeys, a highly effective vaccine against marek's disease (md) in chickens, was ineffective in protecting turkeys against md. another tissue-culture attenuated vaccine virus also protected chickens, but not turkeys, from md. intact and immunosuppressed turkey poults inoculated with herpesvirus of turkey developed a persistent viremia, but did not have detectable gross or microscopic lesions. | 1982 | 6280536 |
| monoclonal antibodies specific to and cross-reactive with marek's disease virus and herpesvirus of turkeys. | in order to identify the specific and cross-reactive antigens to marek's disease virus (mdv) and herpesvirus of turkeys (hvt), we prepared hybridomas producing monoclonal antibodies against these viruses. these monoclonal antibodies were screened by the indirect immunofluorescence method. the purified virus-specific antigens appeared to be more effective immunogens than unpurified virus-infected cell homogenates. totals of 50 and 14 hybridoma clones were found to produce antibodies specific to m ... | 1982 | 6191751 |
| protection by attenuated and polyvalent vaccines against highly virulent strains of marek's disease virus. | tests confirmed that turkey herpesvirus (hvt) vaccine protected chickens poorly against challenge with the highly virulent md5 strain of marek's disease (md) virus, especially in chickens with homologous hvt antibodies. the naturally avirulent sb-1 vaccine virus was likewise poorly protective against challenge with the md5 strain. homologous antibodies reduced the protective efficacy of both vaccines, but sb-1 was not affected by hvt antibodies. in order to provide better protection against stra ... | 1982 | 18770172 |
| characterisation of two highly oncogenic strains of marek's disease virus. | the rb-1b and ala-8 strains of marek's disease (md) virus, which were isolated from chickens with md and which had been vaccinated with the herpesvirus of turkeys (hvt), were evaluated for their oncogenic potential in genetically susceptible (p-line) and resistant (n-line, pdrc) chickens. rb-1b and ala-8 were both highly oncogenic, causing a high incidence of md in both susceptible and resistant birds. vaccination of p-line birds with sb-1 or hvt did not protect satisfactorily against rb-1b. how ... | 1982 | 18770225 |
| herpesvirus infection enhances cholesterol and cholesteryl ester accumulation in cultured arterial smooth muscle cells. | in our previous experiments, atherosclerosis similar to that in humans was reproducibly induced in both normocholesterolemic and hypercholesterolemic specific-pathogen-free (spf) chickens by infection with marek's disease herpesvirus (mdv). in contrast, uninfected chickens fed either relatively cholesterol-poor or cholesterol-supplemented diets did not develop this arterial disease. in experiments reported here, the hypothesis that infection of arterial smooth muscle cells (smcs) with mdv would ... | 1981 | 6271018 |
| natural killer cell activity in chickens exposed to marek's disease virus: inhibition of activity in susceptible chickens and enhancement of activity in resistant and vaccinated chickens. | chickens of 2 genetic lines (lines p and n) were inoculated with a pathogenic strain of marek's disease (md) virus (mdv) and chronologically examined for disease response and natural killer (nk) cell expression. the nk cell reactivity was assayed in an in vitro cytotoxicity assay in which effector cells from the spleen of test chickens were reacted with 51cr-labeled lscc-rp9 target cells. chickens of line p developed progressive debilitating disease and a high incidence of gross tumors and death ... | 1981 | 6279075 |
| latency of herpesvirus of turkey and marek's disease virus genomes in a chicken t-lymphoblastoid cell line. | the properties of latent herpesvirus of turkey (hvt) and marek's disease virus (mdv) genomes have been studied in virus-non-producer mdcc-bo1(t) cells, a t-lymphoblastoid cell line derived from spleen cells of an hvt-vaccinated chicken. the numbers of the two virus genomes in bo1(t) cells remained stable at 1.6 to 1.8 hvt genome equivalents/cell and 3.4 to 3.8 mdv genome equivalents/cell throughout a number of passages and were not decreased by the presence of phosphonoacetic acid in the culture ... | 1981 | 6268738 |
| a simple in vitro differentiation between turkey herpesvirus and marek's disease virus. | the growth and plaque formation by turkey herpesvirus (hvt) amd marek's disease herpesvirus (mdhv) were examined in qt35 cells, a continuous fibroblast cell line derived from chemically induced tumors of japanese quail. hvt grew and formed plaques consistently in qt35 cells when inoculated with cell-culture-propagated virus or peripheral mononuclear leukocytes (pml) from chickens that had been inoculated with hvt. both oncogenic and nononcogenic strains of mdhv, however, failed to grow and induc ... | 1981 | 6279072 |
| latent infections with marek's disease virus and turkey herpesvirus. | the number of plaque-forming units (pfu) from lymphocytes latently infected with oncogenic (ga-5) or nononcogenic (sb-1) marek's disease virus (mdv) was decreased after complement lysis with antisera against thymus-derived lymphocytes or bursa-derived lymphocytes; an additive effect with dual treatment was observed. complement lysis with antisera against marek's disease tumor-associated surface antigen (putative tumor antigen) had little effect, which suggests an absence of the antigen on latent ... | 1981 | 6259403 |
| difference between influences of homologous and heterologous maternal antibodies on response to serotype-2 and serotype-3 marek's disease vaccines. | marek's disease (md) vaccines representing serotypes 2 (sb-1 strain of md virus) and 3 (fc-126 strain of turkey herpesvirus) were administered to 1-day-old md-susceptible chicks that either were free of antibodies or carried maternally derived antibodies against sb-1, fc-126, or a serotype-1 md virus, cu-2 strain. homologous antibodies delayed the development of vaccine virus viremias and inhibited vaccinal immunity, as judged by protection against challenge with the virulent jm-10 strain of md ... | 1981 | 6268052 |
| reduced vaccinal protection of turkey herpesvirus against field strains of marek's disease herpesvirus. | four isolates of marek's disease herpesvirus (mdv) were obtained from 21-week-old broiler breeders with lesions and mortality from marek's disease (md). they had been properly vaccinated with turkey herpesvirus (hvt). the isolates were designated ala-7, 8, 9, and 10. these 4 mdv strains were compared with the ga isolate of mdv in chickens vaccinated with graded doses of hvt. challenge with the mdv strains was also with graded doses. all 5 isolates of mdv were similar in virulence. however, prote ... | 1981 | 7267529 |
| relation between the neutralization of herpesvirus of turkeys and the antibody to late-appearing membrane antigen induced by the virus. | the relation between the neutralization of herpesvirus of turkeys hvt) and the antibodies to early appearing membrane antigen (ema), late appearing membrane antigen (lma) and intracellular antigen (ia) induced by the virus was examined by blocking of direct immunofluorescence using 38 sera from chickens infected with hvt. our results provide evidence that anti-lma is significant in the neutralization of the infectivity (p less than 0.001) since the neutralizing capacity of the serum was related ... | 1980 | 7365465 |
| ultrastructure and infectivity of tissues from normal and immunodepressed chickens inoculated with turkey herpesvirus. | 1980 | 7391278 | |
| computerized reconstruction of the core of herpesvirus of turkey. | 1980 | 7241008 | |
| blocking of immunofluorescence in the titration of membrane and intracellular reactive antibodies associated with herpesvirus of turkeys. | blocking of the direct immunofluorescence (if) reaction allowed titration of anti-membrane antigen (ma) or anti-intracellular antigen (ia) antibodies induced by herpesvirus of turkeys (hvt). the titers (blocking index) obtained by the blocking if test were well correlated with those determined by the if test. the results suggest that the blocking if test was a simpler and better method for titration of a large number of serum samples from chickens infected with hvt. | 1980 | 7002147 |
| long term effects of marek's disease vaccination with cell-free herpesvirus of turkey and age at debeaking on performance and mortality of white leghorns. | three-hundred-sixty, day-old single comb white leghorn chicks were randomly placed in 8 treatment combinations. the combinations consisted of vaccinated or nonvaccinated chicks that were either not debeaked, or debeaked at day-old, 4 weeks old, or 8 weeks old. vaccinated birds received 6,000 plaque forming units of cell-free herpesvirus of turkey vaccine per chick at day-old. debeaking was performed by removing approximately two-thirds of the upper beak. data were analyzed at the 5% level of pro ... | 1980 | 7433359 |
| cell-mediated immunity in marek's disease: cytotoxic responses in resistant and susceptible chickens and relation to disease. | two experiments were conducted to study the cell-mediated cytotoxicity of peripheral blood leukocytes (pbl) from chickens inoculated with marek's disease virus (mdv) against a marek's disease-derived lymphoblastoid cell line (msb-1) and to associate the cytotoxicity with incidence of disease. in experiment i, moderately susceptible random-bred, specific-pathogen-free chickens were inoculated with mdv (group 1), vaccinated with a herpesvirus of turkeys (hvt) and inoculated with mdv (group 2), vac ... | 1980 | 6445171 |
| persistence of genomes of both herpesvirus of turkeys and marek's disease virus in a chicken t-lymphoblastoid cell line. | a cell line tentatively designated as mdcc-bo1(t), was established from spleen cells of an apparently healthy chicken inoculated with herpesvirus of turkey (hvt). bo1(t) cells were t lymphoblastoid cells and the more than 95% of them had marek's disease (md) tumor-associated surface antigen (matsa). however, no viral internal antigens or membrane antigens could be demonstrated in them by immunofluorescence tests using chicken anti-hvt and -md virus (mdv) sera. the virus could be rescued from bo1 ... | 1980 | 6254498 |
| effects of infectious bursal disease on marek's disease vaccination: suppression of antiviral immune response. | studies were made to determine whether infectious bursal disease virus (ibdv) infection would affect the response of chickens to turkey herpesvirus (hvt) vaccination in the development and level of hvt viremia and virus-neutralizing (vn) antibodies to hvt. the hvt viremia in the vaccinated chickens was not affected by ibdv, whether ibdv was inoculated simultaneously with hvt vaccination at one day of age or whether it was inoculated 3 weeks postvaccination with hvt. however, vn antibody response ... | 1980 | 6268042 |
| development and characterization of a marek's disease transplantable tumor in inbred line 72 chickens homozygous at the major (b) histocompatibility locus. | a serially transplantable marek's disease (md) tumor, designated mdct-rp-3, was developed from an md-virus-induced lymphoma (ga strain) in a pedigreed female chicken of the inbred b-histocompatible (b2/b2), line 72, and is the first md tumor transplant to be developed in chickens both syngeneic and selected for susceptibility to md. the mdct-rp-3 tumor maintained its female karyotype through at least 80 passages in male 72 chickens. high doses of tumor cells caused progressively growing tumors a ... | 1980 | 6254488 |
| antigenic difference between intracellular and membrane antigens induced by herpesvirus of turkeys. | the difference between intracellular antigen (ia) and membrane antigen (ma) induced by herpesvirus of turkeys (hvt) was examined by immunofluorescence analysis. convalescent sera from chickens infected with hvt (hvt convalescent serum) and hyperimmune sera from chickens immunized with partially pruified virus (anti-hvt particle serum) or with a major hvt soluble precipitin antigen (anti-common ag serum) were used as the source of antisera to specify both antigens in these experiments. absorption ... | 1980 | 6154448 |
| localization of herpesvirus of turkeys in blood cells. | the buffy coat pellets of blood clots from broad breasted large white turkeys were processed for electron microscopy. no viruses were found in extracellular spaces nor in the heterophils, eosinophils, or basophils. enveloped virions, measuring approximately 135 nm in diameter consisting of a core 30 to 35 nm in diameter and a capsid 20 to 30 nm thick were found in cytoplasmic vacuoles in lymphocytes. no developmental stages of virus maturation were observed in the nuclei or cytoplasm of any of t ... | 1980 | 6251439 |