Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| β-lactamase inhibition by avibactam in mycobacterium abscessus. | two β-lactams, cefoxitin and imipenem, are part of the reference treatment for pulmonary infections with mycobacterium abscessus. m. abscessus has recently been shown to produce a broad-spectrum β-lactamase, blamab, indicating that the combination of β-lactams with a blamab inhibitor may improve treatment efficacy. the objectives of this study were to evaluate the impact of blamab production on the efficacy of β-lactams in vitro and to assess the benefit of blamab inhibition on the activity of β ... | 2015 | 25525201 |
| nontuberculous mycobacteria: skin and soft tissue infections. | skin and soft tissue infections caused by nontuberculous mycobacteria are increasing in incidence. the nontuberculous mycobacteria are environmental, acid-fast bacilli that cause cutaneous infections primarily after trauma, surgery and cosmetic procedures. skin findings include abscesses, sporotrichoid nodules or ulcers, but also less distinctive signs. important species include mycobacterium marinum and the rapidly growing mycobacterium: m. fortuitum, m. abscessus and m. chelonae. obtaining tis ... | 2015 | 26143432 |
| inhaled antibiotics for refractory nontuberculous mycobacteria and non-cystic fibrosis bronchiectasis and the significance of mycobacterium abscessus subsp. abscessus isolation during m. avium complex lung disease therapy. | 2015 | 26131990 | |
| iatrogenic mycobacterium abscessus infection in a trigger finger. | an immunocompetent 63-year-old lady developed mycobacterium abscessus soft tissue infection of the hand following local corticosteroid injection for trigger finger. the patient was successfully treated with repeated radical debridement and prolonged antimicrobial therapy. atypical mycobacterial infections, including those caused by m. abscessus, albeit rare, should be considered in cases of late-onset indolent infection following local injury surgical procedures, and injections. clinical vigilan ... | 2015 | 26117482 |
| ifitms from mycobacteria confer resistance to influenza virus when expressed in human cells. | interferon induced transmembrane proteins (ifitms) found in vertebrates restrict infections by specific viruses. ifitm3 is known to be essential for restriction of influenza virus infections in both mice and humans. vertebrate ifitms are hypothesized to have derived from a horizontal gene transfer from bacteria to a primitive unicellular eukaryote. since bacterial ifitms share minimal amino acid identity with human ifitm3, we hypothesized that examination of bacterial ifitms in human cells would ... | 2015 | 26075508 |
| two episodes of cutaneous non-tuberculous mycobacterial infection in a patient with psoriasis. | non-tuberculous mycobacteria (ntm) are a group of environmental pathogens, which cause a broad spectrum of disease. the incidence of ntm infection is increasing, especially in immunocompromized patients. the past three decades also saw a rapid increase in the incidence of ntm infection involving otherwise healthy subjects. we report a case of cutaneous ntm infection in a 79-year-old chinese woman, who was receiving methotrexate for psoriasis. mycobacterial culture grew mycobacterium abscessus, a ... | 2015 | 26236445 |
| pulmonary disease due to nontuberculous mycobacteria: current state and new insights. | since pulmonary nontuberculous mycobacteria (pntm) lung disease was last reviewed in chest in 2008, new information has emerged spanning multiple domains, including epidemiology, transmission and pathogenesis, clinical presentation, diagnosis, and treatment. the overall prevalence of pntm is increasing, and in the united states, areas of highest prevalence are clustered in distinct geographic locations with common environmental and socioeconomic factors. although the accepted paradigm for transm ... | 2015 | 26225805 |
| chest imaging comparison between non-tuberculous and tuberculosis mycobacteria in sputum acid fast bacilli smear-positive patients. | the aim of this study was to distinguish and compare the chest imaging features of non-tuberculous mycobacteria lung disease (ntm-ld) and pulmonary tuberculosis (ptb) in patients with sputum acid fast bacillus (afb)-smear positive since an earlier differential diagnosis between these two is very important in clinical practice. | 2015 | 26214779 |
| combating highly resistant emerging pathogen mycobacterium abscessus and mycobacterium tuberculosis with novel salicylanilide esters and carbamates. | in the mycobacterium genus over one hundred species are already described and new ones are periodically reported. species that form colonies in a week are classified as rapid growers, those requiring longer periods (up to three months) are the mostly pathogenic slow growers. more recently, new emerging species have been identified to lengthen the list, all rapid growers. of these, mycobacterium abscessus is also an intracellular pathogen and it is the most chemotherapy-resistant rapid-growing my ... | 2015 | 26210507 |
| susceptibility testing of mycobacterium abscessus by isothermal microcalorimetry. | we evaluated a new method for susceptibility testing of a rapidly growing mycobacterium using real-time measurement of heat (microcalorimetry). mics of 2 clinical mycobacterium abscessus isolates were determined by microbroth dilution and e-test. for microcalorimetry, middlebrook-7h10 agar+10% oleic acid-albumin-dextrose-catalase, containing amikacin, clarithromycin, linezolid, and ciprofloxacin was inoculated with ~10(5)cfu/ml. heat production was measured at 37°c for 72h. minimal heat inhibiti ... | 2015 | 26210204 |
| subinhibitory doses of aminoglycoside antibiotics induce changes in the phenotype of mycobacterium abscessus. | subinhibitory doses of antibiotics have been shown to cause changes in bacterial morphology, adherence ability, and resistance to antibiotics. in this study, the effects of subinhibitory doses of aminoglycoside antibiotics on mycobacterium abscessus were investigated. the treatment of m. abscessus cells with subinhibitory doses of amikacin was found to change their colony from a smooth to a rough morphotype and increase their ability to adhere to a polyvinylchloride plate, aggregate in culture, ... | 2015 | 26195529 |
| support from phylogenomic networks and subspecies signatures for separation of mycobacterium massiliense from mycobacterium bolletii. | mycobacterium abscessus subspecies classification has important clinical implications. we used phylogenomic network and amino acid analyses to provide evidence for the separation of mycobacterium bolletii and mycobacterium massiliense into two distinct subspecies which can potentially be differentiated rapidly by their protein signatures. | 2015 | 26157149 |
| otomastoiditis caused by non-tuberculous mycobacteria: report of 16 cases, 3 with infection intracranially. | to analyse the clinical presentation, treatment and outcome in patients diagnosed with otomastoiditis caused by non-tuberculous mycobacteria. | 2015 | 26153833 |
| hydrolysis of clavulanate by mycobacterium tuberculosis β-lactamase blac harboring a canonical sdn motif. | combinations of β-lactams with clavulanate are currently being investigated for tuberculosis treatment. since mycobacterium tuberculosis produces a broad spectrum β-lactamase, blac, the success of this approach could be compromised by the emergence of clavulanate-resistant variants, as observed for inhibitor-resistant tem variants in enterobacteria. previous analyses based on site-directed mutagenesis of blac have led to the conclusion that this risk was limited. here, we used a different approa ... | 2015 | 26149997 |
| non-tuberculous mycobacterial cochlear implant infection: an emerging pathogen. | to report one of the first cases of a cochlear implant infected by non-tuberculous mycobacteria (ntm), an emerging otologic pathogen. | 2015 | 25747861 |
| rapid detection and immune characterization of mycobacterium abscessus infection in cystic fibrosis patients. | cystic fibrosis patients are highly susceptible to infections with non-tuberculous mycobacteria. especially mycobacterium abscessus infections are common but reliable diagnosis is hampered by non-specific clinical symptoms and insensitive mycobacterial culture. in the present study we established novel methods for rapid detection and immune characterization of mycobacterium abscessus infection in cystic fibrosis patients. we performed mycobacterium abscessus specific dna-strip- and quantitative ... | 2015 | 25742660 |
| impact of β-lactamase inhibition on the activity of ceftaroline against mycobacterium tuberculosis and mycobacterium abscessus. | the production of β-lactamases bla(mab) and blac contributes to β-lactam resistance in mycobacterium abscessus and mycobacterium tuberculosis, respectively. ceftaroline was efficiently hydrolyzed by these enzymes. inhibition of m. tuberculosis blac by clavulanate decreased the ceftaroline mic from ≥ 256 to 16 to 64 μg/ml, but these values are clinically irrelevant. in contrast, the ceftaroline-avibactam combination should be evaluated against m. abscessus since it inhibited growth at lower and p ... | 2015 | 25733512 |
| mycobacterium abscessus pneumonia in a south pacific islander. | 2015 | 25731702 | |
| lessons learnt from an atypical mycobacterium infection post-anterior cruciate ligament reconstruction. | infections following anterior cruciate ligament reconstruction are rare, with no previous reports citing mycobacterium abscessus as the culprit pathogen. a 22-year-old man presented twice over three years with a painful discharging sinus over his right tibia tunnel site necessitating repeated arthroscopy and washout, months of antibiotic therapy, and ultimately culminating in the removal of the implants. in both instances, m. abscessus was present in the wound cultures, along with a coinfection ... | 2015 | 25729530 |
| detection of mycobacterium abscessus from deep pharyngeal swabs in cystic fibrosis. | 2015 | 25727723 | |
| treatment of mycobacterium abscessus subsp. massiliense tricuspid valve endocarditis. | 2015 | 25695799 | |
| population-based incidence of pulmonary nontuberculous mycobacterial disease in oregon 2007 to 2012. | pulmonary nontuberculous mycobacteria (ntm) disease is a chronic, nonreportable illness, making it difficult to monitor. although recent studies suggest an increasing prevalence of ntm disease in the united states, the incidence and temporal trends are unknown. | 2015 | 25692495 |
| nontuberculous mycobacterial infections in cystic fibrosis. | nontuberculous mycobacteria (ntm) are important emerging cystic fibrosis (cf) pathogens. factors including the steady aging of the cf population, the apparent increase of ntm in the environment, and the potential for patient-to-patient transmission, may contribute to increased acquisition. diagnosis of ntm disease is challenging due to disease overlap; thus, comprehensive care of the cf patient must be optimized to assess the clinical impact of the ntm (indolent versus active), and to improve re ... | 2015 | 25676523 |
| nontuberculous mycobacteria infections in immunosuppressed hosts. | diseases and therapies that reduce cell-mediated immunity increase the risk of nontuberculous mycobacterial (ntm) disease. extrapulmonary ntm disease, including disseminated, skin, and catheter-related disease, is more common in immunosuppressed than immunocompetent patients. mycobacterium avium complex remains the most common cause of ntm infection, but rapid growers including mycobacterium abscessus, mycobacterium chelonae, and mycobacterium fortuitum play an important role in skin and cathete ... | 2015 | 25676522 |
| the treatment of rapidly growing mycobacterial infections. | rapidly growing mycobacteria (rgm) include a diverse group of species. we address the treatment of the most commonly isolated rgm-m abscessus complex, m fortuitum, and m chelonae. the m abscessus complex is composed of 3 closely related species: m abscessus senso stricto (hereafter m abscessus), m massiliense, and m bolletii. most studies address treatment of m abscessus complex, which accounts for 80% of lung disease caused by rgm and is the second most common rgm to cause extrapulmonary diseas ... | 2015 | 25676520 |
| preliminary results of bedaquiline as salvage therapy for patients with nontuberculous mycobacterial lung disease. | bedaquiline is an oral antimycobacterial agent belonging to a new class of drugs called diarylquinolines. it has low equivalent minimal inhibitory concentrations for mycobacterium tuberculosis and nontuberculous mycobacterial (ntm) lung disease, especially mycobacterium avium complex (mac) and mycobacterium abscessus (mab). bedaquiline appears to be effective for the treatment of multidrug-resistant tb but has not been tested clinically for ntm disease. | 2015 | 25675393 |
| mycobacterium abscessus morphotype comparison in a murine model. | pulmonary infections with mycobacterium abscessus (m. abscessus) are increasingly prevalent in patients with lung diseases such as cystic fibrosis. m. abscessus exists in two morphotypes, smooth and rough, but the impact of morphotype on virulence is unclear. we developed an immune competent mouse model of pulmonary m. abscessus infection and tested the differences in host inflammatory response between the morphotypes of m. abscessus. smooth and rough morphotypes of m. abscessus were isolated fr ... | 2015 | 25675351 |
| crystallization and preliminary x-ray diffraction analysis of a putative carbon-carbon bond hydrolase from mycobacterium abscessus 103. | the phlg protein from mycobacterium abscessus 103 (mphlg), which shares 30% sequence identity with phloretin hydrolase from eubacterium ramulus and 38% sequence identity with 2,4-diacetylphloroglucinol hydrolase from pseudomonas fluorescens pf-5, is a putative carbon-carbon bond hydrolase. here, the expression, purification and crystallization of mphlg are reported. crystals were obtained using a precipitant consisting of 100 mm citric acid ph 5.0, 1.0 m lithium chloride, 8%(w/v) polyethylene gl ... | 2015 | 25664803 |
| clinical significance of nontuberculous mycobacteria from respiratory specimens in stem cell transplantation recipients. | the clinical importance of the isolation of nontuberculous mycobacteria (ntm) from respiratory specimens of stem cell transplant (sct) recipients is not clear. we investigated the characteristics and clinical impact of ntm isolation in this population. medical records of adult patients who underwent sct at the blood and marrow transplantation center at a university hospital in south korea between january 2004 and december 2013 were retrospectively reviewed. twenty-five of 2,658 patients were enr ... | 2015 | 25663510 |
| rapidly growing mycobacteria in singapore, 2006-2011. | nontuberculous mycobacteria infection is a growing global concern, but data from asia are limited. this study aimed to describe the distribution and antibiotic susceptibility profiles of rapidly growing mycobacterium (rgm) isolates in singapore. clinical rgm isolates with antibiotic susceptibility tests performed between 2006 and 2011 were identified using microbiology laboratory databases and minimum inhibitory concentrations of amikacin, cefoxitin, clarithromycin, ciprofloxacin, doxycycline, i ... | 2015 | 25658536 |
| development of a peptide nucleic acid-based multiprobe real-time pcr method targeting the hsp65 gene for differentiation among mycobacterium abscessus strains. | recently, the need to distinguish between members of the mycobacterium abscessus group has gained increasing attention. here, we introduced a novel peptide nucleic acid (pna) real-time pcr method targeting the hsp65 gene in order to distinguish between four subspecies within the m. abscessus group (m. abscessus and 3 types of m. massiliense). | 2015 | 25653415 |
| utility of sequencing the erm(41) gene in isolates of mycobacterium abscessus subsp. abscessus with low and intermediate clarithromycin mics. | the erm(41) gene confers inducible macrolide resistance in mycobacterium abscessus subsp. abscessus, calling into question the usefulness of macrolides for treating m. abscessus subsp. abscessus infections. with an extended incubation (14 days), isolates with mics of ≥8 μg/ml are considered macrolide resistant by current clsi guidelines. our goals were to determine the incidence of macrolide susceptibility in u.s. isolates, the validity of currently accepted mic breakpoints, and the erm(41) sequ ... | 2015 | 25653399 |
| mycobacterium abscessus. "pleased to meet you, hope you guess my name...". | mycobacterium abscessus is a formidable and difficult-to-treat mycobacterial pathogen with multiple drug-resistance mechanisms. the most important of these mechanisms is the presence of an inducible erythromycin methylase (erm) gene, because it confers macrolide resistance. it has recently been found that "m. abscessus" can be split into three species or subspecies based on gene sequence analysis other than the 16s rrna gene and the presence or absence of a functional erm(41) gene. several names ... | 2015 | 25643064 |
| increasing patients with pulmonary mycobacterium avium complex disease and associated underlying diseases in japan. | this study was conducted to evaluate trends in the isolation of strains of nontuberculous mycobacteria (ntm) and trends in the number of patients with pulmonary mycobacterium avium complex (mac) disease. we retrospectively reviewed microbiological results and clinical data to identify patients who were diagnosed with pulmonary mac disease at kyoto university hospital in japan between 2000 and 2013. ntm were isolated from 6327 of 80,285 samples (7.9%) for mycobacterial culture. the proportion of ... | 2015 | 25640532 |
| induction of heme oxygenase-1 contributes to survival of mycobacterium abscessus in human macrophages-like thp-1 cells. | mycobacterium abscessus (m.abs) is a rapidly growing mycobacterial species that infects macrophages, and is an important pathogen in patients with cystic fibrosis. we studied the early stages of m.abs infection of macrophages, with emphasis on the role of heme-oxygenase-1 (ho-1) in this infection. thp-1 cells were activated using tpa into macrophage-like cells and infected with m.abs for different time points. m.abs infection robustly induced ho-1 expression in the thp-1 cells. production of ho- ... | 2015 | 25638774 |
| non-tuberculous mycobacterial infections after solid organ transplantation: a survival analysis. | the relationship of non-tuberculous mycobacterial (ntm) infections and survival among solid organ transplant recipients is unknown. we conducted a retrospective cohort study to measure the impact of ntm infection on survival in this patient population, comparing the effect of mycobacterium abscessus infection with that of infections due to other pathogenic ntm species. we identified 33 patients with ntm infection post-transplantation, 18 with infection that was diagnosed within the first year. a ... | 2015 | 25636926 |
| aminoglycoside-modifying enzymes determine the innate susceptibility to aminoglycoside antibiotics in rapidly growing mycobacteria. | infections caused by the rapidly growing mycobacterium (rgm) mycobacterium abscessus are notoriously difficult to treat due to the innate resistance of m. abscessus to most clinically available antimicrobials. aminoglycoside antibiotics (aga) are a cornerstone of antimicrobial chemotherapy against m. abscessus infections, although little is known about intrinsic drug resistance mechanisms. we investigated the role of chromosomally encoded putative aminoglycoside-modifying enzymes (ame) in aga su ... | 2015 | 25604746 |
| evaluation of the speed-oligo mycobacteria assay for the identification of nontuberculous mycobacteria. | nontuberculous mycobacteria (ntm) causing human infectious disease have become increasingly common. rapid and accurate identification to the species level is, therefore, critical. the speed-oligo mycobacteria assay is an oligochromatographic method that was made available recently for the identification and differentiation of mycobacteria. the present study aimed to evaluate the performance of the speed-oligo mycobacteria assay for the identification of ntm. we examined a total of 62 strains (9 ... | 2015 | 25596120 |
| investigation of the population structure of mycobacterium abscessus complex strains using 17-locus variable number tandem repeat typing and the further distinction of mycobacterium massiliense hsp65 genotypes. | mycobacterium abscessus complex is a significant pathogen in patients with non-cystic fibrosis (non-cf). nevertheless, there is little description of the genetic diversity of this species. the aims of this study were to investigate the distribution of m. abscessus complex isolated from respiratory specimens by variable number tandem repeat (vntr) typing. the results of 104 clinical isolates from 104 non-cf patients were compared using pfge, hsp65 genotypes and clarithromycin susceptibility. the ... | 2015 | 25596119 |
| nontuberculous mycobacterial ocular infections--comparing the clinical and microbiological characteristics between mycobacterium abscessus and mycobacterium massiliense. | to analyze the clinical characteristics of nontuberculous mycobacterial (ntm) ocular infections and the species-specific in vitro antimicrobial susceptibility. | 2015 | 25581038 |
| gallium compounds exhibit potential as new therapeutic agents against mycobacterium abscessus. | the rapidly growing nontuberculous mycobacterial species mycobacterium abscessus has recently emerged as an important pathogen in patients with cystic fibrosis (cf). treatment options are limited because of the organism's innate resistance to standard antituberculous antibiotics, as well as other currently available antibiotics. new antibiotic approaches to the treatment of m. abscessus are urgently needed. the goal of the present study was to assess the growth-inhibitory activity of different g ... | 2015 | 26033732 |
| essential engagement of toll-like receptor 2 in initiation of early protective th1 response against rough variants of mycobacterium abscessus. | although mycobacterium abscessus (m. abscessus) is becoming more prevalent in patients without overt immunodeficiency, little is known about the factors that contribute to disease susceptibility. this study was undertaken to investigate how toll-like receptor 2 (tlr2) functionally contributes to the generation of protective immunity against m. abscessus in a morphotype-specific manner. we found that tlr2-/- mice were extremely susceptible to an intravenous (i.v.) model of infection by m. abscess ... | 2015 | 25644006 |
| mycocap - mycobacterium comparative analysis platform. | mycobacterium spp. are renowned for being the causative agent of diseases like leprosy, buruli ulcer and tuberculosis in human beings. with more and more mycobacterial genomes being sequenced, any knowledge generated from comparative genomic analysis would provide better insights into the biology, evolution, phylogeny and pathogenicity of this genus, thus helping in better management of diseases caused by mycobacterium spp.with this motivation, we constructed mycocap, a new comparative analysis ... | 2015 | 26666970 |
| vaccine strategies against cystic fibrosis pathogens. | a great number of cystic fibrosis (cf) pathogens such as pseudomonas aeruginosa, the burkholderia cepacia and the mycobacterium abscessus complex raised difficult therapeutic problems due to their intrinsic multi-resistance to numerous antibiotics. vaccine strategies represent one of the key weapons against these multi-resistant bacteria in a number of clinical settings like cf. different strategies are considered in order to develop such vaccines, linked either to priming the host response, or ... | 2015 | 26618824 |
| cysteamine as a future intervention in cystic fibrosis against current and emerging pathogens: a patient-based ex vivo study confirming its antimicrobial and mucoactive potential in sputum. | cysteamine has recently been shown to have in vitro properties potentially therapeutically beneficial in cystic fibrosis (cf). in this study we investigated the antimicrobial and mucolytic activity of cysteamine against the complex biologic matrix of cf sputum. | 2015 | 26629546 |
| mycobacterium abscessus complex identification with matrix-assisted laser desorption ionization-time of flight mass spectrometry. | we determined that the vitek ms plus matrix-assisted laser desorption ionization-time of flight mass spectrometry using research-use-only (ruo) v.4.12 and in vitro-diagnostic (ivd) v.3.0 databases accurately identified 41 mycobacterium abscessus subsp. abscessus and 13 m. abscessus subsp. massiliense isolates identified by whole-genome sequencing to the species but not the subspecies level, from middlebrook 7h11 and burkholderia cepacia selective agars. peak analysis revealed three peaks potenti ... | 2015 | 25948607 |
| "you can't always get what you want…" the mycobacterium abscessus issue. name versus function. | 2015 | 25786149 | |
| epidemiology of nontuberculous mycobacteria among patients with cystic fibrosis in scandinavia. | nontuberculous mycobacteria (ntm) are an emerging threat to cystic fibrosis (cf) patients but their epidemiology is not well described. | 2015 | 25178871 |
| emerging bacterial pathogens and changing concepts of bacterial pathogenesis in cystic fibrosis. | chronic suppurative lower airway infection is a hallmark feature of cystic fibrosis (cf). decades of experience in clinical microbiology have enabled the development of improved technologies and approaches for the cultivation and identification of microorganisms from sputum. it is increasingly apparent that the microbial constituents of the lower airways in cf exist in a dynamic state. indeed, while changes in prevalence of various pathogens occur through ageing, differences exist in successive ... | 2015 | 25881770 |
| reduced risk of nontuberculous mycobacteria in cystic fibrosis adults receiving long-term azithromycin. | azithromycin reduces exacerbations in cystic fibrosis (cf) patients. our aim was to investigate its association with nontuberculous mycobacteria isolation and macrolide susceptibility. | 2015 | 25735458 |
| real-time monitoring of hydrogen cyanide (hcn) and ammonia (nh₃) emitted by pseudomonas aeruginosa. | we present the real-time monitoring of hydrogen cyanide (hcn) production from pseudomonas aeruginosa (p. aeruginosa) strains in vitro, using laser-based photoacoustic spectroscopy. simultaneously, the production of ammonia (nh3) was measured, and the influence of different factors (e.g. the medium, temperature and antibiotics treatment) was assessed. both reference strains and clinical isolates of patients with cf were studied, and compared to other pathogens commonly present in lungs/airways of ... | 2015 | 25634638 |
| epidemiology of infection and current guidelines for infection prevention in cystic fibrosis patients. | the spectrum of bacterial pathogens encountered in cystic fibrosis (cf) lung disease has expanded over the last decade. in addition to established pathogens, such as pseudomonas aeruginosa, burkholderia cepacia complex and staphylococcus aureus, novel gram-negative non-fermenter bacteria and non-tuberculous mycobacteria have gained in clinical significance. air sampling performed in inpatient and outpatient clinics, and analysis of cough aerosols expelled by cf patients provides evidence for pot ... | 2015 | 25791927 |
| the challenge of pulmonary nontuberculous mycobacterial infection. | the incidence of nontuberculous mycobacterial (ntm) lung disease is increasing. current treatment strategies are largely based on expert opinion. the lack of randomized clinical trials to inform treatment leave clinicians with many questions regarding the most effective and safe regimens. the risk-benefit ratio of therapy is often thought to favor observation given the chronic nature of the disease, multiple long-term antibiotics recommended for therapy, side effects associated with treatment, a ... | 2015 | 26877911 |
| disseminated mycobacterium abscessus infection following septic arthritis: a case report and review of the literature. | mycobacterium abscessus is a rapidly growing mycobacterium found mainly in patients with respiratory or cutaneous infections, but it rarely causes disseminated infections. little is known about the clinical characteristics, treatment, and prognosis of disseminated m abscessus infection. a 75-year-old japanese woman who had been treated for 17 years with a corticosteroid for antisynthetase syndrome with antithreonyl-trna synthetase antibody developed swelling of her right elbow. x-ray of her righ ... | 2015 | 26020393 |
| [soft-tissue infection with mycobacterium abscessus subsp. abscessus after liposuction and panniculectomy in the caribbean]. | we report the case of a 36-year-old woman who presented with a surgical site infection with atypical mycobacteria several months after liposuction in the caribbean. postoperative wound infections with mycobacterium abscessus present as subcutaneous nodular abscesses with partly putrid secretion. due to the necessity of specific diagnostic measures, diagnosis is often delayed. treatment is difficult because mycobacterium abscessus is resistant to conventional tuberculostatics and many other antib ... | 2015 | 26016828 |
| peritoneal dialysis-associated peritonitis caused by mycobacterium abscessus. | 2015 | 26015424 | |
| mycobacterium abscessus abscess post-thread facial rejuvenation procedure. | 2015 | 25987942 | |
| mycobacterium abscessus pulmonary infection under treatment with tocilizumab. | although biological agents are of considerable benefit to patients with rheumatoid arthritis (ra), the potential for opportunistic infections is a critical issue. it is therefore important to achieve a balance between treatment efficacy and controlling opportunistic infection. we herein report the successfully managed case of a 53-year-old patient with ra who developed pulmonary mycobacterium abscessus infection during treatment with tocilizumab and methotrexate. | 2015 | 25986276 |
| identification of mycobacterium abscessus complex and m. abscessus subsp. massiliense culture isolates by real-time assays. | 2015 | 25976000 | |
| evaluation of the efficacy of novel oxazolidinone analogues against nontuberculous mycobacteria in vitro. | nontuberculous mycobacteria (ntm) are associated with a number of clinical diseases and only a few antitubercular agents are active against them. oxazolidinones comprise a novel class of antimicrobials that inhibit bacterial protein synthesis at the ribosome. linezolid, the first oxazolidinone antibacterial agent approved for clinical use, has excellent activity against some ntm but is ineffective against others. sy142 and sy144 are novel oxazolidinones with demonstrated activties against mycoba ... | 2015 | 25971324 |
| rapid diagnosis of mycobacterium abscessus hand infection by oligonucleotide array in an immunocompetent patient. | 2015 | 25959575 | |
| serodiagnosis of mycobacterium abscessus complex infection in cystic fibrosis. | early signs of pulmonary disease with mycobacterium abscessus complex (mabsc) can be missed in patients with cystic fibrosis (cf). a serological method could help stratify patients according to risk. the objective of this study was to test the diagnostic accuracy of a novel method for investigating igg activity against mabsc.a prospective study of all patients attending the copenhagen cf centre was conducted by culturing for mabsc during a 22-month period and then screening patients with an anti ... | 2015 | 25929948 |
| rapid detection of acquired and inducible clarithromycin resistance in mycobacterium abscessus group by a simple real-time pcr assay. | by targeting the erm(41) and rrl genes in the mycobacterium abscessus group, a multiplex real-time pcr assay for clarithromycin resistance showed 95% (38/40) concordance with nucleic acid testing and 95% (37/39) concordance with phenotypic testing. this assay provides a simple and rapid alternative to extended incubation or erm(41) sequencing. | 2015 | 25903572 |
| increased frequency of nontuberculous mycobacteria detection at potable water taps within the united states. | nontuberculous mycobacteria (ntms) are environmental microorganisms that can cause infections in humans, primarily in the lung and soft tissue. the prevalence of ntm-associated diseases is increasing in the united states. exposure to ntms occurs primarily through human interactions with water (especially aerosolized). potable water from sites across the u.s. was collected to investigate the presence of ntm. water from 68 taps was sampled 4 times over the course of 2 years. in total, 272 water sa ... | 2015 | 25902261 |
| multisite infection with mycobacterium abscessus after replacement of breast implants and gluteal lipofilling. | introduction. medical tourism for aesthetic surgery is popular. nontuberculous mycobacteria (ntm) occasionally cause surgical-site infections. as ntm grow in biofilms, implantations of foreign bodies are at risk. due to late manifestation, infections occur when patients are back home, where they must be managed properly. case report. a 39-year-old healthy female was referred for acute infection of the right gluteal area. five months before, she had breast implants replacement, abdominal liposuct ... | 2015 | 25893122 |
| mycobacterium franklinii sp. nov., a species closely related to members of the mycobacterium chelonae-mycobacterium abscessus group. | two isolates from water, d16q19 and d16r27, were shown to be highly similar in their 16s rrna, 16s-23s internal transcribed spacer (its), hsp65 and rpob gene sequences to 'mycobacterium franklinii' dsm 45524, described in 2011 but with the name not validly published. they are all nonpigmented rapid growers and are related phenotypically and genetically to the mycobacterium chelonae-mycobacterium abscessus group. extensive characterization by phenotypic analysis, biochemical tests, drug susceptib ... | 2015 | 25858242 |
| pediatric cutaneous nontuberculous mycobacterium infections in singapore. | nontuberculous mycobacterium (ntm) infections are rare in children, with limited published studies. the course of the disease can be variable and there are no accepted treatment guidelines for the management of ntm infections in children. | 2015 | 25845296 |
| nontuberculous mycobacteria in cystic fibrosis and non-cystic fibrosis bronchiectasis. | increasing numbers of cystic fibrosis (cf) and non-cf bronchiectasis patients are affected by pulmonary nontuberculous mycobacteria (ntm) infection worldwide. two species of ntm account for up to 95% of the pulmonary ntm infections: mycobacterium avium complex (mac) and mycobacterium abscessus complex (mabsc). diagnosis of pulmonary ntm infection is based on criteria specified in the 2007 american thoracic society/infectious disease society of america (ats/idsa) guidelines. while many initial po ... | 2015 | 25826589 |
| mycobacterium abscessus pulmonary infection complicated with vertebral osteomyelitis in a heart transplant recipient: case report and literature review. | infections produced by mycobacterium abscessus are emerging in immunosuppressed patients, such as solid organ transplant recipients. we report the first case, to our knowledge, of a vertebral osteomyelitis caused by m. abscessus in a heart transplant recipient, and review the risk factors, manifestations, and therapeutic approaches to this uncommon disease. | 2015 | 25816889 |
| cutaneous infections caused by rapidly growing mycobacteria: case reports and review of clinical and laboratory aspects. | skin and soft tissue infections caused by rapidly growing non-tuberculous mycobacteria (rg-ntm) have become a growing clinical concern over the past decades. these rg-ntm are ubiquitous environmental organisms and most are resistant to traditional antituberculous agents. in this report, we describe 3 cutaneous infections caused by rg-ntm, namely, mycobacterium abscessus, m. chelonae, and m. conceptionense, and present the clinical and laboratory characteristics of these infections. | 2015 | 25809023 |
| bacterial phospholipases c as vaccine candidate antigens against cystic fibrosis respiratory pathogens: the mycobacterium abscessus model. | vaccine strategies represent one of the fighting answers against multiresistant bacteria in a number of clinical settings like cystic fibrosis (cf). mycobacterium abscessus, an emerging cf pathogen, raises difficult therapeutic problems due to its intrinsic antibiotic multiresistance. | 2015 | 25804706 |
| human infections due to nontuberculous mycobacteria: the infectious diseases and clinical microbiology specialists' point of view. | nontuberculous mycobacteria (>150 species such as mycobacterium avium, mycobacterium kansasii, mycobacterium chelonae and mycobacterium abscessus) are opportunistic pathogens causing lung and extrarespiratory infections, beside m. ulcerans and m. marinum that are pathogens causing specific skin and soft tissue infections. disseminated infections occur only in severe immunosuppressed conditions such as aids. the diagnosis is based on repeated isolations of the same mycobacterium associated with c ... | 2015 | 26344005 |
| rapid prediction of inducible clarithromycin resistance in mycobacterium abscessus. | we have developed a single tube taqman(®) real-time pcr assay that differentiates the full-length and truncated erm(41) gene to predict inducible resistance to clarithromycin in mycobacterium abscessus. a study of 87 clinical isolates found this assay to be 90.8% concordant to conventional drug susceptibility testing results for the prediction of inducible clarithromycin drug resistance. | 2015 | 26334290 |
| molecular mechanisms of clarithromycin resistance in mycobacterium abscessus complex clinical isolates from venezuela. | in this study, 26 clinical isolates of mycobacterium abscessus complex strains were characterised using available identification algorithms for the three species (m. abscessus, m. massiliense and m. bolletii) and the genotypic characteristics of clarithromycin (clr) resistance were determined. strains were identified by pcr restriction fragment length polymorphism analysis of the hsp65 and erm(41) gene sequences. susceptibility to clr was determined by the broth microdilution method. the mechani ... | 2015 | 27873710 |
| susceptibility of mycobacterium abscessus to antimycobacterial drugs in preclinical models. | over the last 10 years, mycobacterium abscessus group strains have emerged as important human pathogens, which are associated with significantly higher fatality rates than any other rapidly growing mycobacteria. these opportunistic pathogens are widespread in the environment and can cause a wide range of clinical diseases, including skin, soft tissue, central nervous system, and disseminated infections; by far, the most difficult to treat is the pulmonary form. infections with m. abscessus are o ... | 2015 | 26303795 |
| mycobacterium abscessus complex infections in humans. | mycobacterium abscessus complex comprises a group of rapidly growing, multidrug-resistant, nontuberculous mycobacteria that are responsible for a wide spectrum of skin and soft tissue diseases, central nervous system infections, bacteremia, and ocular and other infections. m. abscessus complex is differentiated into 3 subspecies: m. abscessus subsp. abscessus, m. abscessus subsp. massiliense, and m. abscessus subsp. bolletii. the 2 major subspecies, m. abscessus subsp. abscessus and m. abscessus ... | 2015 | 26295364 |
| clonal diversification and changes in lipid traits and colony morphology in mycobacterium abscessus clinical isolates. | the smooth-to-rough colony morphology shift in mycobacterium abscessus has been implicated in loss of glycopeptidolipid (gpl), increased pathogenicity, and clinical decline in cystic fibrosis (cf) patients. however, the evolutionary phenotypic and genetic changes remain obscure. serial isolates from nine non-cf patients with persistent m. abscessus infection were characterized by colony morphology, lipid profile via thin-layer chromatography and matrix-assisted laser desorption ionization-time o ... | 2015 | 26292297 |
| new real-time pcr assays for detection of inducible and acquired clarithromycin resistance in the mycobacterium abscessus group. | members of the mycobacterium abscessus group (mag) cause lung, soft tissue, and disseminated infections. the oral macrolides clarithromycin and azithromycin are commonly used for treatment. mag can display clarithromycin resistance through the inducible erm(41) gene or via acquired mutations in the rrl (23s rrna) gene. strains harboring a truncation or a t28c substitution in erm(41) lose the inducible resistance trait. phenotypic detection of clarithromycin resistance requires extended incubatio ... | 2015 | 26269619 |
| carbapenems and rifampin exhibit synergy against mycobacterium tuberculosis and mycobacterium abscessus. | an effective regimen for treatment of tuberculosis (tb) is comprised of multiple drugs that inhibit a range of essential cellular activities in mycobacterium tuberculosis. the effectiveness of a regimen is further enhanced if constituent drugs act with synergy. here, we report that faropenem (a penem) or biapenem, doripenem, or meropenem (carbapenems), which belong to the β-lactam class of antibiotics, and rifampin, one of the drugs that forms the backbone of tb treatment, act with synergy when ... | 2015 | 26259792 |
| separation of mycobacterium abscessus into subspecies or genotype level by direct application of peptide nucleic acid multi-probe- real-time pcr method into sputa samples. | recently, we introduced a novel peptide nucleic acid (pna) multi-probe real time pcr method targeting the hsp65 gene (hsp65 pna rt-pcr) to distinguish mycobacterium abscessus groups. | 2015 | 26259717 |
| infections caused by rapidly growing mycobacteria spp in children and adolescents with cancer. | rapidly growing mycobacteria (rgm) infections in pediatric oncology patients have not been completely characterized. | 2015 | 26407409 |
| epidemiology of nontuberculous mycobacteria in french polynesia. | as few data are available in the pacific countries and territories of the oceania region regarding nontuberculous mycobacteria,we retrospectively identified 87 such isolates from french polynesia from 2008 to 2013 by hybridization using dna-strip, matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) and partial rpob gene sequencing. partial rpob gene sequencing classified 42/87 (48.3%) isolates in the mycobacterium fortuitum complex, 28 (32.2%) in the mycob ... | 2015 | 26400787 |
| a novel cluster of mycobacterium abscessus complex revealed by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (maldi-tof ms). | mycobacterium abscessus complex is a rapidly growing mycobacterium consisting of 3 subspecies, m. abscessus, mycobacterium massiliense, and mycobacterium bolletii. however, rapid and accurate species identification is difficult. we first evaluated a suitable protocol of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (maldi-tof ms) for distinguishing these subspecies. then, we studied spectral signals by maldi-tof ms in 59 m. abscessus, 42 m. massiliense, and 2 m. bo ... | 2015 | 26388548 |
| deciphering and imaging pathogenesis and cording of mycobacterium abscessus in zebrafish embryos. | zebrafish (danio rerio) embryos are increasingly used as an infection model to study the function of the vertebrate innate immune system in host-pathogen interactions. the ease of obtaining large numbers of embryos, their accessibility due to external development, their optical transparency as well as the availability of a wide panoply of genetic/immunological tools and transgenic reporter line collections, contribute to the versatility of this model. in this respect, the present manuscript desc ... | 2015 | 26382225 |
| mycobacterium saopaulense sp. nov., a rapidly growing mycobacterium closely related to members of the mycobacterium chelonae--mycobacterium abscessus group. | five isolates of non-pigmented, rapidly growing mycobacteria were isolated from three patients and,in an earlier study, from zebrafish. phenotypic and molecular tests confirmed that these isolates belong to the mycobacterium chelonae-mycobacterium abscessus group, but they could not be confidently assigned to any known species of this group. phenotypic analysis and biochemical tests were not helpful for distinguishing these isolates from other members of the m. chelonae–m.abscessus group. the is ... | 2015 | 26358475 |
| [detection of mutations in the gyra and gyrb genes associated with fluoroquinolone-resistance among clinical isolates of mycobacterium abscessus in china]. | to determine molecular mutations in gyra and gyrb genes and their association with fluoroquinone-resistance among mycobacterium abscessus isolates from china. | 2015 | 26703016 |
| post liposuction mycobacterium abscessus surgical site infection in a returned medical tourist complicated by a paradoxical reaction during treatment. | rapidly growing mycobacterial skin and soft tissue infections are known to complicate cosmetic surgical procedures. treatment consists of more surgery and prolonged antibiotic therapy guided by drug susceptibility testing. paradoxical reactions occurring during antibiotic therapy can further complicate treatment of non-tuberculous mycobacterial infections. we report a case of post liposuction mycobacterium abscessus surgical site infection in a returned medical tourist and occurrence of paradox ... | 2015 | 26753088 |
| [sporadic cutaneous infections due to nontuberculous mycobacteria: a retrospective study of 37 cases]. | to study the clinical and pathological characteristics of sporadic cutaneous infections due to nontuberculous mycobacteria (ntm), and investigate the diagnostic criteria and therapeutic principal. | 2015 | 26679654 |
| antibiotic susceptibility of diverse mycobacterium abscessus complex strains in new south wales, australia. | members of the mycobacterium abscessus complex are emerging pathogens of increasing importance, causing both respiratory and soft tissue infections, but precise speciation is problematic. this study was performed to examine the subspecies and antibiotic susceptibility of m. abscessus complex isolates collected during 2013 at the statewide new south wales mycobacterium reference laboratory (nsw mrl), australia. mycobacterium abscessus subsp. abscessus accounted for more than half of all m. absces ... | 2015 | 26517625 |
| chronic pulmonary disease with mycobacterium abscessus complex is a biofilm infection. | 2015 | 26493807 | |
| [a case of pulmonary mycobacterium abscessus infection successfully treated with short-term cam, amk, and ipm/cs followed by long-term oral cam and lvfx]. | a 78-year-old woman who had been treated for two years with itcz for chronic pulmonary aspergillosis associated with prior pulmonary tuberculosis was admitted to our hospital because of general fatigue and hemosputum along with deterioration of her chest radiographic findings. mycobacterium abscessus had been isolated once from her sputum one year before admission. we performed fiberoptic bronchoscopy (fob) in order to help establish a final diagnosis. sputum aspirated from her bronchus on fob s ... | 2015 | 26489157 |
| [a study of surgical treatment for patients with mycobacterium abscessus pulmonary disease and a comparative examination of mycobacterium avium complex disease]. | this is a retrospective study on six surgical cases of mycobacterium abscessus pulmonary disease, including a comparison with m. avium complex (mac) disease. | 2015 | 26477110 |
| high-throughput transcriptomics reveals common and strain-specific responses of human macrophages to infection with mycobacterium abscessus smooth and rough variants. | mycobacterium abscessus (mab) is an emerging pathogen causing pulmonary infections in those with inflammatory lung disorders, such as cystic fibrosis (cf), and is associated with the highest fatality rate among rapidly growing mycobacteria (rgm). phenotypically, mab manifests as either a smooth (mab-s) or a rough (mab-r) morphotype, which differ in their levels of cell wall glycopeptidolipids (gpls) and in their pathogenicity in vivo. as one of the primary immune cells encountered by mab, we sou ... | 2015 | 26654095 |
| amikacin pharmacokinetics/pharmacodynamics in a novel hollow-fiber mycobacterium abscessus disease model. | the treatment of pulmonary mycobacterium abscessus disease is associated with very high failure rates and easily acquired drug resistance. amikacin is the key drug in treatment regimens, but the optimal doses are unknown. no good preclinical model exists to perform formal pharmacokinetics/pharmacodynamics experiments to determine these optimal doses. we developed a hollow-fiber system model of m. abscessus disease and studied amikacin exposure effects and dose scheduling. we mimicked amikacin hu ... | 2015 | 26643339 |
| mycobacterium abscessus in people with cystic fibrosis: considerations for psychosocial care. | 2015 | 26545769 | |
| nontuberculous mycobacterial pulmonary infections. | pulmonary infections due to nontuberculous mycobacteria (ntm) are increasingly recognized worldwide. although over 150 different species of ntm have been described, pulmonary infections are most commonly due to mycobacterium avium complex (mac), mycobacterium kansasii, and mycobacterium abscessus. the identification of these organisms in pulmonary specimens does not always equate with active infection; supportive radiographic and clinical findings are needed to establish the diagnosis. it is dif ... | 2014 | 24624285 |
| lung transplantation in patients with cystic fibrosis: special focus to infection and comorbidities. | despite advances in medical care, patients with cystic fibrosis still face limited life expectancy. the most common cause of death remains respiratory failure. end-stage cystic fibrosis can be treated with lung transplantation and is the third most common reason for which the procedure is performed. outcomes for cystic fibrosis are better than most other lung diseases, but remain limited (5-year survival 60%). for patients with advanced disease lung transplantation appears to improve survival. o ... | 2014 | 24655065 |
| mycobacterium abscessus infection in a burn intensive care unit patient. | infection is the leading cause of death in burn patients. historically, this was due to burn wound sepsis but pneumonia has now emerged as the most common source. in light of the increasing incidence of multi-drug-resistant organisms, the description of rare infections is paramount in continuing the fight against deadly pathogens. we aim to describe the second case of non-tuberculous mycobacterium (ntm) reported in a burn patient. difficulties in diagnosis and management will also be highlighted ... | 2014 | 25493353 |
| antibiotic management of lung infections in cystic fibrosis. ii. nontuberculous mycobacteria, anaerobic bacteria, and fungi. | airway infections are a key component of cystic fibrosis (cf) lung disease. whereas the approach to common pathogens such as pseudomonas aeruginosa is guided by a significant body of evidence, other infections often pose a considerable challenge to treating physicians. in part i of this series on the antibiotic management of difficult lung infections, we discussed bacterial organisms including methicillin-resistant staphylococcus aureus, gram-negative bacterial infections, and treatment of multi ... | 2014 | 25167882 |
| contamination of hypertonic saline solutions in use by cystic fibrosis patients in israel. | treatment of cystic fibrosis (cf) patients with inhaled hypertonic saline (hs) solutions is safe, beneficial and reduces exacerbation rates. we studied contamination of solutions used by israeli cf patients for prolonged periods. | 2014 | 24484849 |