Publications

detection of four plasmodium species in blood from humans by 18s rrna gene subunit-based and species-specific real-time pcr assays.	there have been reports of increasing numbers of cases of malaria among migrants and travelers. although microscopic examination of blood smears remains the "gold standard" in diagnosis, this method suffers from insufficient sensitivity and requires considerable expertise. to improve diagnosis, a multiplex real-time pcr was developed. one set of generic primers targeting a highly conserved region of the 18s rrna gene of the genus plasmodium was designed; the primer set was polymorphic enough int ...	2004	15583293
evaluation of the realart malaria lc real-time pcr assay for malaria diagnosis.	pcr-based methods have advantages over traditional microscopic methods for the diagnosis of malaria, especially in cases of low parasitemia and mixed infections. however, current pcr-based assays are often labor-intensive and not readily quantifiable and have the potential for contamination due to a requirement for postamplification sample handling. real-time pcr can address these limitations. this study evaluated the performance characteristics of a commercial malaria real-time pcr assay (reala ...	2004	14766829
a systematic overview of published antimalarial drug trials.	systematic database searches identified 435 antimalarial drug treatment trials, involving 82,616 patients, conducted and published between 1966 and december 2002. of these trials 72% were randomised; 64 (15%) trials involved severe malaria, 47 (11%) studied plasmodium vivax, 3 plasmodium malariae or plasmodium ovale, and the remainder (74%) assessed treatment responses in uncomplicated falciparum malaria. twelve trials (2.7%) specifically evaluated antimalarial treatments in pregnant women. over ...	2004	14964805
malaria relapse and recrudescence among travellers to the tropics.	this study describes 14 cases of relapse and recrudescence of malaria, treated between 1991 and 2003. in that period, 146 patients were hospitalized in the clinic of the institute in gdynia: 20 women and 126 men. in 103 cases the disease was caused by plasmodium falciparum, in 31 cases by plasmodium vivax, in 5 cases by plasmodium malariae, and in 2 cases by plasmodium ovale. five patients were found to have mixed infections, with either p. falciparum and p. vivax or p. falciparum and p. ovale. ...	2003	14974782
epidemiology of highland malaria in western kenya.	to investigate the epidemiology of falciparum malaria in workers from a highland tea plantation in western kenya with very seasonally limited malaria transmission to determine what factors are associated with increased risk of malaria transmission in the kenyan highlands.	2003	16167741
problems in the therapy for imported malaria in the united states.	errors in the diagnosis of imported malaria are increasingly recognized. however, there are few data on the treatment of malaria in the united states.	2003	14504115
plasmepsin 4, the food vacuole aspartic proteinase found in all plasmodium spp. infecting man.	plasmepsins are aspartic proteinases of the malaria parasite, and seven groups of plasmepsins have been identified by comparing genomic sequence data available for the genes encoding these enzymes from plasmodium falciparum, plasmodium vivax, plasmodium knowlesi, plasmodium berghei, and plasmodium yoelii. the food vacuole plasmepsins typified by plasmepsin 4 from p. falciparum (pfpm4) constitute one of these groups. genes encoding the ortholog of pfpm4 have been cloned from plasmodium ovale, pla ...	2003	14550891
procalcitonin serum levels in tertian malaria.	procalcitonin (pct) is closely correlated with parasite burden and clinical outcome in falciparum malaria. the role of pct in tertian malaria has not previously been investigated.	2003	14613513
external quality assessment schemes raise standards: evidence from the ukneqas parasitology subschemes.	the burden of parasitic disease imported into the temperate zone is increasing, and in the tropics remains very high. thus, high quality diagnostic parasitology services are needed, but to implement clinical governance a measure of quality of service is required.	2003	14645352
transmission of mixed plasmodium species and plasmodium falciparum genotypes.	we studied malaria transmission by comparing parasite populations in humans and mosquito vectors at the household level. blood samples were collected from all inhabitants for microscopic detection of gametocytes and polymerase chain reaction analysis. the next morning, blood-fed resting mosquitoes were collected inside the bed nets used by the individuals surveyed the previous afternoon. after 8 days of maintenance, mosquitoes were dissected, and midguts and salivary glands were recovered for po ...	2003	12641406
automated detection of malaria-associated intraleucocytic haemozoin by cell-dyn cd4000 depolarization analysis.	laboratory tests for malaria are only performed if there is clinical suspicion of the disease, and a missed diagnosis contributes substantially to morbidity and mortality. malaria parasites produce haemozoin, which is able to depolarize light and this allows the automated detection of malaria during routine complete blood count analysis (cbc) with some abbott cell-dyn instruments. in this study, we evaluated the cell-dyn cd4000 with 831 blood samples submitted for malaria investigations. samples ...	2003	12641610
avoiding misdiagnosis of imported malaria: screening of emergency department samples with thrombocytopenia detects clinically unsuspected cases.	misdiagnosis of imported malaria is not uncommon and even abnormal routine laboratory tests may not trigger malaria smears. however, blind screening of all thrombocytopenic samples might be a possible way to detect clinically unsuspected malaria cases in the accident and emergency department (aed).	2003	12757689
cross-species regulation of plasmodium parasitemia in semi-immune children from papua new guinea.	malariologists have long been fascinated by the question of whether plasmodium spp. interact in the human host. the first genetic study of the longitudinal dynamics of multiple plasmodium spp. and genotypes in humans has been completed in papua new guinea, where all four plasmodium spp. that infect humans are present. the broad implications of the data from this study are covered here and they show that the total parasite density of plasmodium species oscillates around a threshold and that peaks ...	2003	12798085
use of a rapid, single-round, multiplex pcr to detect malarial parasites and identify the species present.	a new, rapid assay, based on a single-round, multiplex pcr, can be used to detect plasmodium falciparum, p. vivax, p. malariae or p. ovale in human blood. the pcr, which targets the conserved 18s small-subunit rna genes of the parasites, not only permits a malarial infection to be detected but also allows each plasmodium species present to be identified, even in cases of mixed infection.	2003	12803868
[triple malaria infection in twin sisters from the democratic republic of congo].	infections with 3 species of malaria parasites are rarely encountered and observed in less than 0.05% of cases. we came across such an infection in four year-old, monozygote twin sisters, coming from kinshasa (democratic republic of congo). in both of them, parasitemia was low or very low for p. falciparum and p. ovale and of 0.1-0.2% for p. malariae. the twin sisters presented with an iron deficiency anaemia, associated with an heterozygous sickle-cell anaemia and a moderate splenomegaly. the b ...	2003	12836524
wide distribution of plasmodium ovale in myanmar.	the presence of plasmodium ovale has never been previously reported in myanmar. using blood samples obtained in many villages across the country between 1996 and 2000, molecular diagnosis of plasmodium species was made with semi- or full-nested polymerase chain reaction (pcr) with species-specific primers, followed by agarose gel electrophoresis to detect amplification products. the presence of p. ovale was also confirmed with the another pcr-based diagnosis, the microtiterplate hybridization (m ...	2002	11903985
[imported malaria in children in 1999. study of the armand-trousseau hospital in paris].	eighty children were treated at the hospital armand-trousseau (paris, france) for a malaria attack from 1999-01-01 to 2000-02-01.	2002	11998422
two types of plasmodium ovale defined by ssu rrna have distinct sequences for ookinete surface proteins.		2002	12106877
diagnostic criteria and risk factors for plasmodium ovale malaria.	plasmodium ovale is a common malaria parasite in africa, but the epidemiology of p. ovale malaria is poorly known. exposure to malaria, parasitemia, and morbidity were monitored for 6 years among the residents of a village in senegal. the relationship between the level of p. ovale parasitemia and fever risk were analyzed, and diagnostic criteria for clinical p. ovale malaria were established. then the relationships between the occurrence of p. ovale clinical malaria and a series of entomological ...	2002	12195357
a retrospective examination of sporozoite-induced and trophozoite-induced infections with plasmodium ovale: development of parasitologic and clinical immunity during primary infection.	a retrospective analysis was made of clinical and parasitologic parameters in patients with induced plasmodium ovale infection to document the initial clinical and parasitologic response and their subsequent development of clinical and parasitologic immunity, and to determine the effect of previous homologous and heterologous malaria on subsequent infection with this parasite. the prepatent periods were relatively uniform. eight patients injected with sporozoites that had been stored frozen had ...	2002	12201582
[two cases of mixed infection of malaria diagnosed by pcr method].	we here reported two japanese cases of mixed infections of plasmodium species, whose dnas were detected using the pcr test. one case was a 31 year-old male, who presented fever and fatigue, and had a travel history to kenya, cameroon and indonesia. smear test of his peripheral blood found the presence of plasmodium vivax, while nested-pcr diagnosis detected the dnas both p. vivax and plasmodium malariae. the other was a 54 year-old female suffering from general fatigue. she had been treated with ...	2002	12212325
[current data on malaria in metropolitan france].	epidemiological data from the french national reference center for imported diseases showed that the estimated number of cases of imported malaria in france increased from 5,940 in 1998 to 7,127 in 1999 and 8,056 in 2000. this three-year progression ended in 2001 when the number of estimated cases fell back to 7,223. it was due mainly to the concomitant increase in the number of people traveling to endemic zones especially in africa. in 2000 the median age of patients with imported malaria in fr ...	2002	12244913
the assessment of antimalarial drug efficacy.	antimalarial drug efficacy in uncomplicated malaria should be assessed parasitologically in large, community-based trials, enrolling the age groups most affected by clinical disease. for rapidly eliminated drugs, a 28-day follow-up is needed, but, for slowly eliminated drugs, up to nine weeks could be required to document all recrudescences, and, when possible, the drug levels should also be measured. the who 14-day assessments are neither sensitive nor specific. in tropical plasmodium vivax and ...	2002	12377597
detection of imported malaria with the cell-dyn 4000 hematology analyzer.	the sensitivity and specificity of the cell-dyn 4000 hematology analyzer in the diagnosis of imported malaria were studied with samples from patients in an academic hospital setting. the performance of the cell-dyn 4000 hematology analyzer was compared with that of conventional diagnostic methods for malaria. the cell-dyn 4000 hematology analyzer detected hemozoin-containing depolarizing monocytes in 29 of 58 patients with malaria and 2 of 55 patients without malaria. the presence or absence of ...	2002	12454179
rapid immunochromatographic malarial antigen detection unreliable for detecting plasmodium malariae and plasmodium ovale.	in order to determine the reliability of two commercial tests for the rapid detection of plasmodial antigen in cases of infection with plasmodium ovale and plasmodium malariae, the products were evaluated in four centers and a search of the relevant literature was performed. the results of the present and previous studies were compared. with overall sensitivities ranging between 18.8% and 47.6% for plasmodium malariae and between 20% and 31.3% for plasmodium ovale, it is evident that neither tes ...	2002	12461593
plasmodium ovale malaria acquired in central spain.	we describe a case of locally acquired plasmodium ovale malaria in spain. the patient was a spanish woman who had never traveled out of spain and had no other risk factors for malaria. because patients with malaria may never have visited endemic areas, occasional transmission of malaria to european hosts is a diagnostic and clinical challenge.	2002	12498674
effect of primaquine standard dose (15 mg/day for 14 days) in the treatment of vivax malaria patients in thailand.	primaquine (8-aminoquinoline), the only effective drug to prevent relapses of the persistent liver forms of plasmodium vivax and plasmodium ovale, can induce hemolytic anemia in patients with glucose-6-phosphate dehydrogenase (g6pd) deficiency. the severity varies considerably among affected individuals. three hundred and sixty-four plasmodium vivax cases (342 g6pd-normal and 22 g6pd-deficient) were given a 3-day course of chloroquine (total dose 1,500 mg) followed by primaquine 15 mg a day for ...	2001	12041544
malaria epidemiological situation in italy and evaluation of malaria incidence in italian travelers.	malaria was endemic throughout the country until it was eradicated nearly 50 years ago. since then, mainly imported malaria cases have been reported to the national health service, with an increasing trend. the aim of this study was to present a detailed analysis of the current epidemiological situation of malaria in italy, and to make a first attempt to calculate the incidence of malaria in italian international travelers.	2001	11182603
imported malaria treated in melbourne, australia: epidemiology and clinical features in 246 patients.	imported malaria is increasing in nonendemic countries, including australia. the objective of this study was to describe the epidemiology and clinical features of travelers with imported malaria presenting to a specialist infectious diseases hospital.	2001	11285166
presence of three distinct ookinete surface protein genes, pos25, pos28-1, and pos28-2, in plasmodium ovale.		2001	11295191
parasitic procrastination: late-presenting ovale malaria and schistosomiasis.	a 29-year-old woman with ovale malaria (most likely contracted, together with asymptomatic schistosomiasis, in east africa two years previously) had fever, nausea and confusion, jaundice, anaemia, thrombocytopenia, hyponatraemia and hypokalaemia. she was initially diagnosed with and treated for blood-smear-positive vivax malaria. because of the unusual clinical presentation, blood was analysed by a malaria species-specific nested polymerase chain reaction (pcr) assay which identified plasmodium ...	2001	11548081
[malaria in georgian republic].	due to the prevailing natural conditions, georgia is an endemic country for malaria. all types were widespread in the country, with the exception of plasmodium ovale malaria. a campaign of large-scale and comprehensive measures undertaken by the authorities lead to complete elimination of malaria as an indigenous disease in 1970.	2001	11548306
detection of plasmodium ovale by the ict malaria p.f/p.v. immunochromatographic test.		2001	11700187
erythrocyte fy antigen phenotyping helps differentiate so-called benign tertian malarias.	isolated cases of malaria are increasing in frequency in nonendemic countries. blood film examination remains a mainstay of diagnosis of these sporadic cases because immunologic and molecular methods are unavailable, expensive, and problematic. two tertian malarial species, plasmodium vivax and plasmodium ovale, may appear to be similar morphologically. plasmodium ovale infection is infrequent, and misdiagnosis of this species is common. plasmodium vivax infection can be ruled out, however, if a ...	2000	10656744
[increase of malaria among migrants in amsterdam-zuidoost].	in a general practice in amsterdam southeast in 1998 a delayed first attack of plasmodium ovale infection was diagnosed in a 13-year-old girl from ghana, malaria tropica with a low parasitaemia index in a 43-year-old ghanaian man and a 8-year-old ghanaian girl, and plasmodium vivax infection in a 44-year-old surinam woman. the ghanaian patients had visited their native country, the surinam woman had contracted the infection during a visit to india. all patients responded well to antimalaria medi ...	2000	10674108
difficulties in the prevention, diagnosis, and treatment of imported malaria.	imported malaria is quite common in the united states. increasing antimalarial drug resistance and changes in travel patterns may have important implications for the prevention, clinical presentation, and management of this disease.	2000	10979063
a study of the urban malaria transmission problem in khartoum.	a study of malaria prevalence and transmission was carried out in khartoum, the capital of sudan. the sentinel sites were el manshia, an urban area on the blue nile and ed dekheinat, a lower-income peri-urban area bordering the white nile. anopheles arabiensis, the only malaria vector encountered, was present throughout the year although vector density varied seasonally. plasmodium falciparum was the only species found in el manshia. in ed dekheinat p. falciparum, plasmodium ovale and plasmodium ...	2000	10708656
authors' reply to "plasmodium ovale infection".		2000	11262998
plasmodium ovale malaria in delhi.	a case of ovale malaria in a child from delhi is reported. urban malaria ecotypes caused by p. ovale has never been seen before. characteristic morphological features of the parasite in stained blood film confirmed its identification.	1999	10798048
"plasmodium ovale infection in delhi" published in indian journal of pediatrics jan-feb 1999; vol. 66 (1): 143-145.		1999	10798097
plasmodium ovale infections detected by pcr assay in lao pdr.	plasmodium ovate infection was demonstrated in 5 out of 143 inhabitants in a village in lao pdr by blood microscopy and pcr assay. although the specimen confirmed to be positive for p. ovale by microscopical examination was only one, the target sequences in the 18s rrna genes of malaria parasite detected in all of the five cases were consisted with those of p. ovale by the pcr assay. this is the first report concerning the presence of so many cases with p. ovale infection in lao pdr.	1999	10928349
[diagnostic test to identify human plasmodium species by the quantitative buffy coat test].	the quantitative buffy coat system (qbc test) was designed for rapid diagnosis of malaria by identifying the presence of hemoparasites. the main drawback of the technique is failure to identify the plasmodium species. the purpose of this study was to attempt to remedy this problem by studying the distribution of the parasites at the bottom of the test tube. indeed since the qbc test is based on gradient centrifugation of blood components, the distribution of the parasites in the test tube depend ...	1999	10701207
multispecies plasmodium infections of humans.	we analyzed point-prevalence data from 19 recent studies of human populations in which either plasmodium ovale or plasmodium vivax co-occur with plasmodium falciparum and plasmodium malariae. although the only statistical interactions among, sympatric congeners are pairwise, the frequencies of mixed-species infections relative to standard hypotheses of species sampling independence show no strong relation to overall malaria prevalence. the striking difference between the p. falciparum-p. malaria ...	1999	10207356
malaria prevention in travelers.	the prevention of malaria in travelers is becoming a more challenging clinical and public health problem because of the global development of drug-resistant plasmodium strains of malaria and the increasing popularity of travel to exotic locales. travelers can reduce their risk of acquiring malaria by using bed netting, wearing proper clothing and applying an insect repellent that contains n,n-diethyl-meta-toluamide. chloroquine, once the standard agent for weekly malaria prophylaxis, is no longe ...	1999	10323359
rare quadruple malaria infection in irian jaya indonesia.	we report an exceptional finding from a blood slide collected in a remote area in the western half of new guinea island (irian jaya province, indonesia). one adolescent patient was found patently coinfected with all 4 known human malaria species, plasmodium falciparum, plasmodium vivax, plasmodium malariae, and plasmodium ovale. diagnostic erythrocytic stages of all 4 species were clearly seen in the peripheral blood. a nested polymerase chain reaction, using species-specific primer pairs to det ...	1999	10386460
imported tertian malaria resistant to primaquine.	in plasmodium vivax and plasmodium ovale malaria, some of the liver stage parasites remain dormant. the activation of these dormant forms (called hypnozoite) can give rise to relapse weeks, months or years after the initial infection. to prevent relapses, a course of primaquine may be given as terminal prophylaxis to patients. different strains of plasmodium vivax vary in their sensitivity to primaquine and, recently, cases of relapse of plasmodium vivax after this standard primaquine therapy we ...	1999	10461431
[rapid resolution of plasmodium ovale malarial attacks using artesunate (arsumax)].	this randomized, non-comparative clinical trial without placebo was carried out to assess the efficacy and tolerance of artesunate for treatment of acute plasmodium ovale malarial attacks. thirty cameroonese patients were included. all presented acute plasmodium ovale malarial attacks with parasitemia in excess of 500 asexual forms per mm3. four days after treatment with artesunate, all 30 patients were asymptomatic with no parasitemia. reduction rates were 93.9 p. 100 for asexual forms and 75.4 ...	1999	10472581
how prevalent are plasmodium ovale and p. malariae in east asia?	plasmodium ovale and plasmodium malariae, two of the four human malaria parasites, are usually found at very low prevalence in east asia, even in areas with intense malaria transmission. in this article, fumihiko kawamoto, qing liu, marcelo ferreira and indah tantular review data obtained in recent field surveys, using alternative diagnostic methods such as acridine orange staining and pcr-based methods, to evaluate the prevalence of these two malaria species in east asia. they argue that these ...	1999	10481157
usefulness of seminested multiplex pcr in surveillance of imported malaria in spain.	the use of a new pcr-based method for the diagnosis of malaria in the spanish malaria reference laboratory has promoted an increase in confirmed cases of malaria. from august 1997 to july 1998, a total of 192 whole-blood samples and 71 serum samples from 168 patients were received from the hospitals of the spanish national health system. most of the patients came from west-central african countries (85%). this molecular method showed more sensitivity and specificity than microscopy, detecting 12 ...	1999	10488189
plasmodium ovale in southern africa.		1999	10492808
cloning and characterization of iron-containing superoxide dismutase from the human malaria species plasmodium ovale, p. malariae and p. vivax.	the iron-containing superoxide dismutase (fesod) gene from three human malaria species, namely plasmodium ovale, p. malariae and p. vivax, was amplified by polymerase chain reaction, cloned and then sequenced. comparisons of their deduced amino acid sequences with that of the fesod from p. falciparum revealed a very low polymorphism at the fesod locus in human malaria species. one p. ovale and the p. vivax fesod genes presented the same nucleotide sequence as that of the p. falciparum strain hb3 ...	1999	10599926
high prevalence of plasmodium malariae and plasmodium ovale in malaria patients along the thai-myanmar border, as revealed by acridine orange staining and pcr-based diagnoses.	the prevalence of the four human malaria parasites was investigated among malaria patients at northern, central and southern towns in thailand along the border with myanmar between september 1995 and may 1996. thin smears obtained from 548 thai and burmese patients were reviewed by an acridine orange staining method, and many mixed infections with two to four species, including p. malariae and p. ovale, were detected. these diagnostic results were compared with those by two pcr-based diagnoses, ...	1998	9623932
plasmodium ovale species in papua new guinea--lest we forget.	the microscopical diagnosis of plasmodium ovale infection is reviewed and its similarity to plasmodium vivax emphasized. its presence in papua new guinea has been recognized for many years, from a time not long after stephens first described the species in 1922, but it is rarely reported. there is no doubt of its presence in papua new guinea, together with p. falciparum, p. vivax and p. malariae, but its exact prevalence and distribution has not been determined.	1998	10934553
non-immune patients in the democratic republic of são tomé e principe reveal a high level of transmission of p. ovale and p. vivax despite low frequency in immune patients.	malaria parasite species circulating in immune inhabitants of the democratic republic of são tomé e principe (drstp) and those acquired by non-immune travellers returning from this country have been compared. using sensitive detection and species identification by pcr, plasmodium parasites were found in the blood of 16 of the 43 travellers, who reported during the first 8 months of 1995 to a clinical diagnosis laboratory in lisbon. plasmodium vivax was found in four (25%) and plasmodium ovale in ...	1998	9698266
plasmodium ovale in a highly malaria endemic area of senegal.	during 4 months, from june to september 1990, the population of dielmo village, senegal, an area of intense and perennial malaria transmission, was enrolled in a follow-up study including daily clinical surveillance and bi-weekly malaria parasitaemia monitoring. thick blood film examinations indicated that 48.5% of children (49/101) and 32.4% of adults (34/105) were infected at least once by plasmodium ovale during the study period; 148 distinct episodes of patent parasitaemia were observed, wit ...	1998	9861368
plasmodium ovale in south africa.		1998	9861386
retrospective study of malaria cases treated in newcastle general hospital between 1990 and 1996.	malaria, in particular falciparum malaria, continues to pose a substantial risk to travelers to endemic areas.	1998	9876194
plasmodium ovale in lao pdr.		1997	9656397
pyronaridine for treatment of plasmodium ovale and plasmodium malariae infections.	the clinical efficacy of oral pyronaridine was assessed in 22 symptomatic cameroonian patients infected with plasmodium ovale or plasmodium malariae. all patients were cured on or before day 4. in vitro drug assays confirmed the sensitivity of p. ovale and p. malariae isolates to chloroquine and pyronaridine.	1997	9333075
detection of plasmodium ovale malaria parasites by species-specific 18s rrna gene amplification.	a polymerase chain reaction (pcr) assay was developed for the specific detection of plasmodium ovale, one of the four malaria parasites that infect humans. on the basis of sequence variation of the plasmodium 18s ribosomal rna (rrna) gene, oligonucleotide primers for pcr were designed to amplify various fragments of the p. ovale gene. using a recombinant plasmid with the complete p. ovale 18s rrna gene as target, 59 primer combinations were tested so that at least one of the pairs was species-sp ...	1997	9500806
a variant of plasmodium ovale; analysis of its 18s ribosomal rna gene sequence.	we report here a new variant of human malaria parasite found by comparison of diagnostic results obtained from a new dna diagnostic method named microtiter plate-hybridization (mph) and traditional microscopic method. total five cases of malaria were diagnosed as microscopy-positive but mph-negative; one case was found in epidemiological research in vietnam and four cases were obtained from imported malaria in japan. although they were quite similar to typical p. ovale morphologically in microsc ...	1997	9586115
sequence variation in the 18s rrna gene, a target for pcr-based malaria diagnosis, in plasmodium ovale from southern vietnam.	field surveys of malaria were performed in southern vietnam by using an acridine orange staining method for rapid diagnosis and a pcr-based, microtiter plate hybridization method for accurate diagnosis. a total of three patients of plasmodium ovale infection were detected, but pcr-amplified dna of the p. ovale isolates from two of the patients did not hybridize with the p. ovale-specific probe. analysis of the target sequence in the 18s rrna gene indicated that in the dna of isolates from both p ...	1996	8862600
short report: rosette formation in plasmodium ovale infection.	red blood cells infected by mature stages of plasmodium ovale obtained from a 56-year-old thai patient formed rosettes readily with uninfected erythrocytes. ex vivo, the ring stage-infected erythrocytes matured well under the in vitro conditions used for p. falciparum culture, and the infected erythrocytes formed rosettes when the parasites became mature trophozoites. these rosettes were stable and remained intact until completion of schizogony. plasmodium ovale rosettes were similar to those fo ...	1996	8940990
atovaquone plus proguanil is an effective treatment for plasmodium ovale and p. malariae malaria.		1996	9015517
post-transfusion acquired malaria complicating orthotopic liver transplantation.	early infectious complications within the first 3 months of orthotopic liver transplantation are common and are associated with significant morbidity and mortality. here we report the first case of transfusion-acquired malaria in an orthotopic liver transplantation recipient. the patient was found to have plasmodium ovale malaria during evaluation of a severe febrile illness. the infection was traced to a platelet transfusion and responded to treatment with chloroquine. risk factors associated w ...	1996	8607511
[malaria and drug resistance].	drug resistance is a major problem in malaria. the resistance mechanism remains unresolved but contributing factors are probably heavy drug use, parasite selection, cross resistance and genetic influences of drugs. plasmodium ovale en p. malariae are sensitive to the current antimalarial drugs. p. vivax has some chloroquine resistant strains, notably on papua new guinea, irian jaya and other islands in the pacific. the geographical distribution of p. falciparum strains resistant to proguanil and ...	1996	8618636
the nigerian i/cdc strain of plasmodium ovale in chimpanzees.	the chimpanzee is the only animal host currently available that can support the development of the human malaria parasite plasmodium ovale. thirty-one infections with the nigerian i/cdc strain were induced in splenectomized chimpanzees. maximum parasite counts ranged from 1,240 to 127,224/microliters. infections were transient and unpredictable. anopheles stephensi, anopheles gambiae, anopheles freeborni, and anopheles dirus mosquitoes were infected by feeding through parafilm membranes on hepar ...	1996	8636850
dna diagnosis of malaria using microtiter plate-hybridization.	we have developed a new diagnostic method "microtiter plate-hybridization" (mph) for the detection of human malaria parasite in which the target dna sequence of the 18s ribosomal rna gene is amplified by polymerase chain reaction and hybridized with the species-specific probes immobilized on a microtiter well. the pcr products bound on a well are visualized by the biotin-streptavidin system and the following chromogenic reaction. this method has allowed us to detect and identify the four species ...	1995	8841641
plasmodium: genus-conserved primers for species identification and quantitation.	stable rnas have regions of primary sequence that are nearly identical in every member of the plasmodium genus and not found in the host or in other common pathogens. several "genus-conserved" sequences, which flank hypervariable regions, were identified within the small subunit ribosomal rna of plasmodium species. primers based on these conserved sequences permit amplification of species- or possibly even strain-specific sequences from samples of unknown composition. as an example of this appro ...	1995	7556560
[a case of plasmodium ovale malaria with thrombocytopenia and an abnormality grade in fdp concentration despite the use of chloroquine as a malaria prophylaxis].	a 45-year-old japanese male, who had been to the central african republic, was admitted to our hospital because of high fever with chills on july 29, 1994. he used chloroquine as a malaria prophylaxis during his stay and for 6 weeks after his return to japan. on admission, plasmodium ovale was detected in his blood smears and in the dna analysis. he was treated successfully with chloroquine (1500 mg over 3 day period) and primaquine (15 mg/day for 14 days beginning day 4). disappearance of malar ...	1995	7751755
inoculum size and severity of malaria induced with plasmodium ovale.	although it might be expected that the size of the infecting inoculum would influence the severity of the consequent malaria, evidence that it does so is scarce. using records from 80 non-immune neurosyphilis patients who received malaria therapy with a single strain of plasmodium ovale by blood inoculation, the relationships between the number of trophozoites inoculated, the prepatent period, and measures of severity of the resulting malaria were examined. the number of trophozoites was not rel ...	1995	7785527
current status and prospects of malaria vaccines.	malaria is a parasitic disease caused by four plasmodium species infecting humans, plasmodium falciparum, plasmodium vivax, plasmodium ovale, and plasmodium malariae. the parasite is transmitted by the anopheles mosquitoes that flourish in warm climates. these vectors thus are present in many tourist areas of the world, including africa, asia, and south america. known in china, as far back as 2700 bc, malaria was controlled by medicinal plants or drugs. this was until drug resistance occurred, b ...	1995	9815369
accuracy of routine laboratory diagnosis of malaria in the united kingdom.	to study the accuracy of routine laboratory diagnosis of malaria with the aim of improving accuracy in diagnosis in the future.	1994	7962629
pharmacokinetics and kinetic-dynamic modeling of an 8-aminoquinoline candidate anticyanide and antimalarial drug (wr242511).	malaria is a major cause of health problems in a large portion of the world. the 8-aminoquinoline compound, primaquine, is one of the only compounds useful for relapses of plasmodium vivax and plasmodium ovale malaria. primaquine has several toxicities that include methemoglobinemia and hemolytic anemia. the induction of methemoglobinemia is a treatment for cyanide poisoning. we studied the pharmacokinetics and pharmacodynamics (percentage methemoglobin) for wr242511, an 8-aminoquinoline primaqu ...	1994	8070311
in vitro cultivation of exoerythrocytic stages of the simian malaria parasites plasmodium fieldi and plasmodium simiovale in rhesus monkey hepatocytes.	exoerythrocytic stage parasites of plasmodium fieldi and plasmodium simiovale, 2 simian malaria parasites related to the human malaria parasite plasmodium ovale, were cultured in vitro by inoculating primary cultures of hepatocytes from rhesus monkeys (macaca mulatta) with sporozoites. less than 1% of sporozoites developed into schizonts for either species. structure and size of the liver stages in both species were similar to previous in vivo descriptions, and the time required for in vitro mat ...	1994	8195940
plasmodium ovale: observations on the parasite development in saimiri monkey hepatocytes in vivo and in vitro in contrast with its inability to induce parasitemia.	exoerythrocytic stage parasites of the human malaria parasite plasmodium ovale were cultured in vitro by inoculating primary cultures of hepatocytes from saimiri sciureus boliviensis monkeys with sporozoites. morphology and size of the liver stages were similar to previous in vivo descriptions in humans and chimpanzees. saimiri monkeys did not develop parasitemia after repeated inoculations with p. ovale sporozoites. however, liver-stage parasites were observed in liver biopsies performed 7 days ...	1994	8206138
plasmodium ovale in the highlands of irian jaya, indonesia.		1992	1449279
prophylaxis for malaria. helping world travelers come home healthy.	malaria is largely preventable, so travelers should be taught general protective measures and given appropriate chemoprophylaxis before they leave on their trip. chloroquine phosphate (aralen) is still the drug of choice in locations where malaria remains chloroquine-sensitive. however, chloroquine-resistant areas infested with plasmodium falciparum are becoming more numerous. in such areas, mefloquine hydrochloride (lariam), doxycycline, or proguanil (paludrine) (obtainable outside the united s ...	1992	1518752
spontaneous splenic rupture due to plasmodium ovale malaria.		1991	1681259
late plasmodium ovale malaria.		1991	1682724
recurrent plasmodium ovale infection from papua new guinea--chloroquine resistance or inadequate primaquine therapy?		1991	1753151
plasmodium ovale in indonesia.	we report 34 infections by plasmodium ovale found among 15,806 blood film examinations taken between 1973 and 1989 from several sites in indonesia. twenty five of the p. ovale infections occurred in a single sample of 514 people living in owi, irian jaya. we detected five additional infections at 3 other sites in irian jaya. other infections by p. ovale occurred at two sites in west flores. another infection has already been reported from east timor. despite relatively frequent sampling of popul ...	1990	2151542
[imported malaria at hospitals in bordeaux (france) in 1987 and 1988. report of 185 cases].	100 cases of imported malaria were registered in hospitals of bordeaux in 1987 and 85 in 1988. origin of infestation is africa in 88 p.c. of the cases mainly from central africa (50 p.c.) in 1987 and western africa (67 p.c.) in 1988. the most frequent species was plasmodium falciparum 73 p.c. in 1987 and 82 p.c. in 1988. admitted cases in bordeaux were mainly french (81 p.c.) with a significant male prevailing and young adults. mean age was 29 for europeans and 19 for the others mainly africans. ...	1990	2195286
detection of plasmodium ovale in koraput district, orissa state.	we report for the first time the detection of p. ovale infection in three patients in two hilly villages of koraput district, orissa state, india. the identification of the parasite was confirmed by the imperial colleage of science and technology, london.	1989	2737703
[transfusion malaria caused by plasmodium ovale. a case].		1988	2968562
circumsporozoite protein of the human malaria parasite plasmodium ovale identified with monoclonal antibodies.	monoclonal antibodies (mabs) have been produced against plasmodium ovale sporozoites and used to characterize the circumsporozoite (cs) protein. six mabs were produced, and all were species specific. by using western blot (immunoblot) analysis, three polypeptides were detected: a predominant 51,000-mr polypeptide and two presumed precursor 57,000- and 67,000-mr molecules. the presence of a repeating epitope in the cs protein of p. ovale was demonstrated by using one of the mabs in a single-antib ...	1988	3338845
plasmodium ovale: in vitro development of hepatic stages.	primary cultures of human hepatocytes, a culture-derived clone from the human hepatoma hep g2 line, and cultured rat hepatocytes were inoculated in vitro with plasmodium ovale sporozoites extracted from anopheles stephensi, an. gambiae, and an. dirus mosquitoes. penetration and differentiation of p. ovale sporozoites into trophozoite stage parasites occurred in all three cell types, but with a lower transformation rate in the hep g2 cell line than in the primary cultured hepatocytes. further mat ...	1987	3678445
localization of circumsporozoite protein of plasmodium ovale in midgut oocysts.	circumsporozoite (cs) proteins are the major proteins found on the surface of salivary gland sporozoites and are the protective antigens of several species of malaria parasites. little is known about the distribution of cs proteins in developing oocysts, however. immunoelectron microscopy with protein a-gold and a monoclonal antibody specific for the cs protein of plasmodium ovale was performed to investigate the distribution of cs protein within developing p. ovale oocysts. there was an almost ...	1987	3679537
plasmodium ovale infections in natal.		1987	3686286
infection of chimpanzees with nigerian i/cdc strain of plasmodium ovale.	seven splenectomized chimpanzees were infected with the nigerian i/cdc strain of plasmodium ovale. two of the animals had no history of previous malarial infection whereas three had been infected with p. vivax, one with p. malariae, and one with p. vivax and p. malariae. the two animals with no previous malarial experience had maximum parasitemias of 88,700 and 127,000 per mm3 while the other animals had maximum parasitemias ranging from 10,100 to 60,600 per mm3. anopheles freeborni, an. dirus, ...	1987	3688304
seven patients with relapses of plasmodium vivax or p. ovale despite primaquine treatment.	seven relapses of plasmodium vivax or plasmodium ovale despite standard treatment with primaquine (3.0 mg kg-1) daily for fourteen days are presented. the majority of patients came from areas outside the countries where resistance to primaquine is well known. the various possibilities of reasons for relapses are discussed.	1987	3299660
relapsing malaria infection acquired in kenya.	an american physician-traveler to east africa presented with manifestations of cerebral malaria and was treated with intravenous quinidine for chloroquine-resistant falciparum malaria. he later relapsed with plasmodium ovale infection, despite previous primaquine therapy. treatment of chloroquine-resistant malaria is discussed. the difficulty in diagnosing p. ovale infections and the predominance of this malaria species over p. vivax in east africa are reviewed.	1987	3300055
[anemic plasmodium ovale malaria after 45 months' incubation].		1987	2950492
cloning and characterization of an abundant plasmodium knowlesi antigen which cross reacts with gambian sera.	a 110 kda plasmodium knowlesi antigen, termed pk110, has been identified on the basis of messenger rna abundance in late schizonts. most plasmodium genes previously cloned have been identified by immune sera, which have selected immunodominant antigens composed of repeating epitopes. although pk110 was not selected by immune sera, it also contains amino acid repeats, indicating that this structure may be a common feature of malarial proteins. determination of 296 codons in the pk110 gene reveale ...	1987	2444886
concentration of plasmodium ovale- and plasmodium vivax-infected erythrocytes from nonhuman primate blood using percoll gradients.	plasmodium vivax and plasmodium ovale schizont-infected erythrocytes were separated from peripheral blood by centrifugation using discontinuous percoll (colloidal silica) gradients. infected aotus monkey or chimpanzee blood was diluted and placed on a discontinuous gradient containing 30%, 40%, 45%, and 50% percoll (v/v in media) layers before centrifugation at 1,450 x g. parasitized erythrocytes were concentrated to greater than 95% schizont-infected cells in two bands that contained an average ...	1986	3006527
ultrastructure of erythrocytic stages of plasmodium ovale in humans.	an electron microscopic study of plasmodium ovale in humans supplied information on the fine structure of erythrocytic trophozoites, presegmenting schizonts and gametocytes. ultrastructural features of p. ovale appeared quite similar to those described in other mammalian malarial parasites. however, some minor structural variations were observed. the parasite was a fairly compact cell of an oval shape. however, occasionally trophozoites had slender cytoplasmic protrusions. ingestion of host cell ...	1986	3728791
ultrastructure of human erythrocytes infected with plasmodium ovale.	ultrastructural changes of the human erythrocytes infected with plasmodium ovale were studied, and differences of p. ovale from other human malarial parasites were discussed. four characteristic morphological alterations were observed on the host cells: caveola-vesicle complex, excrescence, nodule, and cytoplasmic cleft. caveola-vesicle complexes consisted of caveolae surrounded by vesicles in an alveolar fashion and were formed along the host cell plasmalemma. similar complexes have been report ...	1986	3728792
stage-specific and species-specific antigens of plasmodium vivax and plasmodium ovale defined by monoclonal antibodies.	monoclonal antibodies (mabs) were produced against the asexual blood stages of plasmodium vivax and plasmodium ovale and used to define antigens of plasmodial parasites in an indirect fluorescent antibody assay. the anti-p. vivax mabs produced two distinct patterns in the indirect fluorescent antibody assay. four patterns were found with the anti-p. ovale mabs. species-specific epitopes were defined for p. vivax and p. ovale; epitopes shared among all four species of human malaria parasites were ...	1986	3536743
haemostatic alterations in malaria correlate to parasitaemia.	fibrin(ogen) degradation products, platelet counts, antithrombin iii, and the components of the factor viii complex were studied in a total of 80 patients with plasmodium falciparum, plasmodium vivax or plasmodium ovale infections. the haemostatic findings were correlated to the numbers of parasitized erythrocytes and to each other. the results indicate that haemostatic changes in malaria correlate with the degree of parasitaemia. evidence for moderate hyperfibrinolysis was found in patients wit ...	1985	3933596
monoclonal antibody identifies circumsporozoite protein of plasmodium malariae and detects a common epitope on plasmodium brasilianum sporozoites.	we produced a hybridoma secreting an immunoglobulin g1 monoclonal antibody against the circumsporozoite protein of the human malaria parasite plasmodium malariae (uganda 1/cdc). the monoclonal antibody produces a circumsporozoite precipitation reaction when incubated with viable sporozoites of p. malariae and reacts at high titers with heat-fixed sporozoites in an indirect immunofluorescent antibody test. using the purified monoclonal antibody and western blot analysis, we identified two polypep ...	1984	6381308