Publications
Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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sediment microbes of deep-sea bioherms on the northwest shelf of australia. | the northwest shelf of australia is a region with known petroleum reserves. recent geological investigations have revealed carbonate knolls postulated to be of biological origin. sediment microbial populations at three sites on the northwest shelf, pee shoal, mermaid reef, and scott reef, were investigated for the presence of hydrocarbon-degrading bacteria. from two sampling trips, 246 bacterial strains were purified, and 182 of these strains displayed preferential growth on hydrocarbon-selectiv ... | 2003 | 12739077 |
contamination of bone marrow products with an actinomycete resembling microbacterium species and reinfusion into autologous stem cell and bone marrow transplant recipients. | bacterial contamination of bone marrow or peripheral blood stem cell transplant products typically occurs with skin flora or, rarely, gram-negative organisms. we describe a clonal outbreak of contamination in transplant products caused by contamination with an aerobic actinomycete that occurred at our institution during the summer of 2001. from 1 july through 12 september 2001, 73 peripheral blood or bone marrow stem cell products were obtained from 39 patients, and 34 products were found to be ... | 2003 | 12746790 |
development and application of an assay for uranyl complexation by fungal metabolites, including siderophores. | an assay to detect uo(2)(2+) complexation was developed based on the chrome azurol s (cas) assay for siderophores (b. schwyn and j. b. neilands, anal. biochem. 160:47-56, 1987) and was used to investigate the ability of fungal metabolites to complex actinides. in this assay the discoloration of two dyed agars (one containing a cas-fe(3+) dye and the other containing a cas-uo(2)(2+) dye) caused by ligands was quantified. the assay was tested by using the siderophore desferrioxamine b (dfo), and t ... | 2003 | 12788768 |
pair-dependent co-aggregation behavior of non-flocculating sludge bacteria. | two strains of non-flocculating sewage sludge bacteria (xanthomonas sp. s53 and microbacterium esteraromaticum s51) showed 91% and 77% co-aggregation, respectively, with acinetobacter johnsonii s35 using a spectrophometric assay. the co-aggregates in case of xanthomonas sp. s53 and a. johnsonii s35 were above 100 microm and stable against edta (2 mm) and a commercial protease (0.2 mg ml(-1)). protease/periodate pretreatment of the partners did not affect this co-aggregation. on the other hand, c ... | 2003 | 12889835 |
initial characterization of new bacteria degrading high-molecular weight polycyclic aromatic hydrocarbons isolated from a 2-year enrichment in a two-liquid-phase culture system. | to characterize some polycyclic aromatic hydrocarbons (pah)-degrading microorganisms isolated from an enriched consortium degrading high molecular weight (hmw) pahs in a two-liquid-phase (tlp) soil slurry bioreactor, and to determine the effect of low molecular weight (lmw) pah on their growth and hmw pah-degrading activity. | 2003 | 12534823 |
interactions between strains of 2,4-diacetylphloroglucinol-producing pseudomonas fluorescens in the rhizosphere of wheat. | abstract strains of fluorescent pseudomonas spp. that produce the antibiotic 2,4-diacetylphoroglucinol (2,4-dapg) are among the most effective rhizobacteria controlling diseases caused by soilborne pathogens. the genotypic diversity that exists among 2,4-dapg producers can be exploited to improve rhizosphere competence and biocontrol activity. knowing that d-genotype 2,4-dapg-producing strains are enriched in some take-all decline soils and that p. fluorescens q8r1-96, a representative d-genotyp ... | 2003 | 18943865 |
diversity of 746 heterotrophic bacteria isolated from microbial mats from ten antarctic lakes. | microbial mats, growing in antarctic lakes constitute unique and very diverse habitats. in these mats microorganisms are confronted with extreme life conditions. we isolated 746 bacterial strains from mats collected from ten lakes in the dry valleys (lakes hoare and fryxell), the vestfold hills (lakes ace, druzhby, grace, highway, pendant, organic and watts) and the larsemann hills (lake reid), using heterotrophic growth conditions. these strains were investigated by fatty acid analysis, and by ... | 2002 | 12583721 |
purification and characterization of a novel esterase promising for the production of useful compounds from microbacterium sp. 7-1w. | a novel esterase catalyzing regioselective hydrolysis was purified from the membrane fraction of microbacterium sp. 7-1w, and characterized. the enzyme was solubilized with brij 58 and purified 13.8-fold to apparent homogeneity with 2.58% overall recovery. the relative molecular mass of the native enzyme as estimated by gel filtration was more than 600,000 da, and the subunit molecular mass was 62,000 da. the enzyme catalyzed cleavage of the terminal ester bonds of cetraxate esters and pantothen ... | 2002 | 11814667 |
biodiversity of the bacterial flora on the surface of a smear cheese. | the bacteria on the surface of a farmhouse smear-ripened cheese at four stages of ripening (4, 16, 23, and 37 days) from inoculated (i.e., deliberately inoculated with brevibacterium linens bl2) and noninoculated (not deliberately inoculated with b. linens bl2) cheese were investigated. the results show that, contrary to accepted belief, b. linens is not a significant member of the surface flora of smear cheese and no microbial succession of species occurred during the ripening of the cheeses. o ... | 2002 | 11823224 |
selective enrichment and characterization of a phosphorus-removing bacterial consortium from activated sludge. | under alternating aerobic/anaerobic conditions and without additional carbon sources, a bacterial consortium consisting initially of 18 bacterial strains was obtained in a sequence batch reactor. the phosphorus removal capability could only be maintained using sterile filtrate of activated sludge as medium. the addition of calcium and magnesium salts, as well as vitamins and trace elements, to autoclaved sterile filtrate of activated sludge was not sufficient to achieve stable phosphorus removal ... | 2002 | 11831470 |
aerobic and facultatively anaerobic cellulolytic bacteria from the gut of the termite zootermopsis angusticollis. | to demonstrate the occurrence of cellulolytic bacteria in the termite zootermopsis angusticollis. | 2002 | 11849325 |
arsenic (iii) oxidizing microbacterium lacticum and its use in the treatment of arsenic contaminated groundwater. | to develop a microbially-assisted process for the removal of arsenic from contaminated groundwater. | 2002 | 11940155 |
isolation and characterization of endophytic colonizing bacteria from agronomic crops and prairie plants. | endophytic bacteria reside within plant hosts without causing disease symptoms. in this study, 853 endophytic strains were isolated from aerial tissues of four agronomic crop species and 27 prairie plant species. we determined several phenotypic properties and found approximately equal numbers of gram-negative and gram-positive isolates. in a greenhouse study, 28 of 86 prairie plant endophytes were found to colonize their original hosts at 42 days postinoculation at levels of 3.5 to 7.7 log(10) ... | 2002 | 11976089 |
purification and characterization of pyridoxal 4-dehydrogenase from aureobacterium luteolum. | a pyridoxal dehydrogenase was purified to homogeneity from aureobacterium luteolum, which can use pyridoxine as a carbon and nitrogen source, and characterized. the enzyme was a dimeric protein with a subunit molecular weight of 38,000. it had several properties distinct from those of the partially purified enzyme from pseudomonas ma-1. the optimum ph (8.0-8.5) was 0.8-1.3 lower than that of the pseudomonas enzyme. the aureobacterium enzyme showed much higher and lower affinities for nad+ (km, 0 ... | 2002 | 12005047 |
prevalent bacterial species and novel phylotypes in advanced noma lesions. | the purpose of this study was to determine the bacterial diversity in advanced noma lesions using culture-independent molecular methods. 16s ribosomal dna bacterial genes from dna isolated from advanced noma lesions of four nigerian children were pcr amplified with universally conserved primers and spirochetal selective primers and cloned into escherichia coli. partial 16s rrna sequences of approximately 500 bases from 212 cloned inserts were used initially to determine species identity or close ... | 2002 | 12037085 |
expression, purification and characterization of a recombinant levan fructotransferase. | a 1.6 kb dna fragment including the lftm gene, encoding a levan fructotransferase (lftase) of microbacterium sp. al-210, was subcloned into a high-expression vector, pet-29b, and the recombinant enzyme was overexpressed in escherichia coli. most of the lftase activity was detected in the cytoplasmic fraction after induction with isopropyl beta-d-thiogalactoside. the recombinant enzyme with a tag of six histidine residues at the c-terminus was purified 132-fold by affinity and gel-filtration chro ... | 2002 | 12074698 |
catheter-related microbacterium bacteremia identified by 16s rrna gene sequencing. | we describe the application of 16s rrna gene sequencing in defining two cases of catheter-related microbacterium bacteremia. in the first case, a gram-positive bacillus was isolated from both the blood culture and central catheter tip of a 39-year-old woman with chronic myeloid leukemia. the api coryne system identified the isolate as 98.9% aureobacterium or corynebacterium aquaticum. in the second case, a gram-positive bacillus was recovered from five sets of blood cultures from both central ca ... | 2002 | 12089308 |
purification, molecular cloning, and characterization of pyridoxine 4-oxidase from microbacterium luteolum. | pyridoxine 4-oxidase (ec 1.1.3.12, pn 4-oxidase), which catalyzes the oxidation of pn by oxygen or other hydrogen acceptors to form pyridoxal and hydrogen peroxide or reduced forms of the acceptors, respectively, was purified for the first time to homogeneity from microbacterium luteolum yk-1 (=aureobacterium luteolum yk-1). the purified enzyme required fad for its catalytic activity and stability. the enzyme was a monomeric protein with the subunit molecular mass of 53,000 +/- 1,000 da. pn was ... | 2002 | 12092811 |
cloning and characterization of a levanbiohydrolase from microbacterium laevaniformans atcc 15953. | an extracellular levanbiohydrolase gene, levm, from microbacterium laevaniformans atcc 15953 was cloned and its nucleotide sequence was determined. nucleotide sequence analysis of this gene revealed a 1863 bp open reading frame coding for a protein of 621 amino acids. the deduced amino acid sequence of the levm gene exhibited 28-47% sequence identities with levanases, levanfructotransferases, and inulinases. the levm was overexpressed by using a t7 promoter in escherichia coli bl21 (de3) and pur ... | 2002 | 12095678 |
plasmid-borne genes code for an angular dioxygenase involved in dibenzofuran degradation by terrabacter sp. strain yk3. | the genes responsible for angular dioxygenation of dibenzofuran in actinomycetes were cloned by using a degenerate set of pcr primers designed by using conserved sequences of the dioxygenase alpha subunit genes. one sequence of alpha subunit genes was commonly amplified from four dibenzofuran-utilizing actinomycetes: terrabacter sp. strains yk1 and yk3, rhodococcus sp. strain yk2, and microbacterium sp. strain yk18. a 5.2-kb psti fragment encoding the alpha and beta subunits of the terminal diox ... | 2002 | 12147464 |
microbacterium aerolatum sp. nov., isolated from the air in the 'virgilkapelle' in vienna. | three rod-shaped, gram-positive strains were isolated from the air of the chapel 'virgilkapelle' in vienna. a representative of these three strains, strain v-73t, shared the highest 16s rdna sequence similarities with members of the genus microbacterium, in particular microbacterium foliorum, microbacterium testaceum, microbacterium esteraromaticum, microbacterium keratanolyticum and microbacterium arabinogalactanolyticum. the strains displayed almost identical biochemical and physiological char ... | 2002 | 12148633 |
microbiology of acute otitis externa. | to isolate and characterize bacteria and fungi from acute otitis externa (aoe) and to obtain susceptibility profiles on each bacterial isolate. | 2002 | 12169893 |
isolation and characterization of dibenzofuran-degrading actinomycetes: analysis of multiple extradiol dioxygenase genes in dibenzofuran-degrading rhodococcus species. | sixteen actinomycetes capable of utilizing dibenzofuran as a sole source of carbon and energy were isolated, including rhodococcus, microbacterium, and terrabacter genera. heretofore, no dibenzofuran-utilizing strain belonging to the genus microbacterium has been reported. five extradiol dioxygenase genes (dfdb, and edil to 4) of the strain rhodococcus sp. yk2 were cloned and analyzed. the nucleotide sequence of dfdb gene was almost identical to the bphc1 gene of terrabacter sp. dpo360, which wa ... | 2002 | 12224629 |
microbial growth-promotion activity of 3-hydroxymonoazine- and n-hydroxydiazine-type heterocycles. | three 3-hydroxymonoazine- and three n-hydroxydiazine-type heterocycles were tested whether they act as artificial siderophores toward aureobacterium flavescens jg-9 (atcc no. 25091). among them, 1-hydroxy-3,5,6-trimethyl-2(1h)-pyrazinone (3) showed the highest growth-promotion activity comparable to desferrioxamine b (dfb), a natural trihydroxamate siderophore, at 48.5 microm or above, followed by 1-hydroxy-5,6-dimethyl-2(1h)-pyrazinone (2), 1-hydroxy-4,6-dimethyl-2(1h)-pyrimidinone (1), and 3-h ... | 2002 | 12235861 |
isolation and characterization of efficient isoxaben-transforming microbacterium sp strains from four european soils. | nutrient-agar plates containing isoxaben (500 mg litre(-1)) were used to isolate isoxaben-metabolising bacteria from four european soils incubated with the herbicide under laboratory conditions. in flask experiments, inoculation of a basal salts medium containing nitrogen and [phenyl-u-14c]isoxaben with an isolate (b2b) resulted in 33% recovery of the initial radioactivity as [14c]carbon dioxide after 2 weeks. a major metabolite identified by gc-ms and nmr analysis as 3-(1-ethyl-1-methylpropyl)i ... | 2002 | 12476996 |
exposure to airborne microorganisms and endotoxin in a potato processing plant. | microbiological air sampling was performed in a big potato processing plant located in eastern poland. air samples for determination of concentrations of microorganisms, dust and endotoxin were collected at 6 sites in the division producing potato flakes and meal from dried potato pulp and at 2 sites in the division producing potato syrup from imported starch. the concentrations of total airborne microorganisms were within a range of 28.3-93.1 x 10(3) cfu/m(3). mesophilic bacteria were dominant ... | 2002 | 12498592 |
investigations on rhizoplane actinobacteria communities of papyrus (cyperus papyrus) from an egyptian wetland. | wetlands have important global ecological functions, which include carbon storage and water interception. wetland contributes to the maintenance of regional and global biodiversity. though many important wetland ecological functions are based on microbial metabolism, we have scanty knowledge on microbial diversity in wetlands. plant rhizoplane habitats are considered to harbor highly diverse bacterial communities. most of the floating mats on river nile are dominated by papyrus (cyperus papyrus) ... | 2002 | 12512252 |
enumeration and characterization of cellulolytic bacteria from refuse of a landfill. | enumeration and phenotypic characterization of aerobic cellulolytic bacteria were performed on fresh, 1 year old and 5 years old refuse samples of a french landfill site. numbers of cellulolytic bacteria ranged from 1.1x10(6) to 2.3x10(8) c.f.u. (g dry wt.)(-1) and were lower in 5 years old refuse samples. a numerical analysis of phenotypic data based on 80 biochemical tests and performed on 321 gram-positive isolates from refuse, revealed a high phenotypic diversity of cellulolytic bacteria whi ... | 2001 | 11137603 |
siderophore mediated plutonium accumulation by microbacterium flavescens (jg-9). | uptake of plutonium and uranium mediated by the siderophore desferrioxamine-b (dfob) has been studied for the common soil aerobe microbacterium flavescens(jg-9). m. flavescens does not bind or take up nitrilotriacetic acid (nta) complexes of u(vi), fe(iii), or pu(iv) or u(vi)-dfob but does take up fe(iii)-dfob and pu(iv)-dfob. pu(iv)-dfob and fe(iii)-dfob accumulations are similar: only living and metabolically active bacteria take up these metal-siderophore complexes. the fe(iii)-dfob and pu(iv ... | 2001 | 11478246 |
description of microbacterium foliorum sp. nov. and microbacterium phyllosphaerae sp. nov., isolated from the phyllosphere of grasses and the surface litter after mulching the sward, and reclassification of aureobacterium resistens (funke et al. 1998) as microbacterium resistens comb. nov.. | the taxonomic position of a group of coryneform bacteria isolated from the phyllosphere of grasses and the surface litter after sward mulching was investigated. on the basis of restriction analyses of 16s rdna, the isolates were divided into two genotypes. according to the 16s rdna sequence analysis, representatives of both genotypes were related at a level of 99.2% similarity and clustered within the genus microbacterium. chemotaxonomic features (major menaquinones mk-12, mk-11 and mk-10; predo ... | 2001 | 11491322 |
nosocomial outbreak of microbacterium species bacteremia among cancer patients. | to date, only 6 sporadic microbacterium species (formerly coryneform centers for disease control and prevention [cdc] groups a-4 and a-5) infections have been reported. the source, mode of transmission, morbidity, mortality, and potential for nosocomial transmission of microbacterium species remain unknown. from 26 july through 14 august 1997, 8 episodes of coryneform cdc group a-5 symptomatic bacteremia occurred in 6 patients on the oncology ward at the maine medical center. one patient died. a ... | 2001 | 11517437 |
molecular and enzymatic characterization of a levan fructotransferase from microbacterium sp. al-210. | microbacterium sp. al-210 producing a novel levan fructotransferase (lftase) was screened from soil samples. the lftase was purified to homogeneity by (nh4)2so4 fractionation, column chromatography on resource q, and superdex 200hr. the molecular weight of the purified enzyme was estimated to be approximately 46 kda by both sds-page and gel filtration, and the enzyme's isoelectric point was ph 4.8. the major product produced from the levan hydrolysis by the enzyme reaction was identified by atmo ... | 2001 | 11522362 |
chromate reduction and 16s rrna identification of bacteria isolated from a cr(vi)-contaminated site. | a gram-positive, hexavalent chromium [chromate: cr(vi)]-tolerant bacterium, isolated from tannery waste from pakistan, was identified as a microbacterium sp. by 16s rrna gene sequence homology. the strain (designated as mp30) reduced toxic cr(vi) only under anaerobic conditions at the expense of acetate as the electron donor. the bacterium was able to grow aerobically in l-broth supplemented with 15 mm cro4(2-) but then did not reduce cr(vi). at a concentration of 2.4x10(9) cells/ml, 100 microm ... | 2001 | 11693930 |
microbacterium gubbeenense sp. nov., from the surface of a smear-ripened cheese. | phenotypic and phylogenetic studies were performed on 11 strains of a microbacterium-like organism isolated from the surface of a smear-ripened cheese. the isolates were gram-positive, catalase-positive, facultatively anaerobic, oxidase-negative, non-spore-forming, non-motile, small, slender rods and grew in 12% (w/v) nacl. chemotaxonomic investigation revealed that all the isolates belonged unambiguously to the genus microbacterium. they contained type b1 peptidoglycans with l-lysine as the dia ... | 2001 | 11760936 |
resident aerobic microbiota of the adult human nasal cavity. | recent evidence strongly suggests that the microbiota of the nasal cavity plays a crucial role in determining the reaction patterns of the mucosal and systemic immune system. however, little is known about the normal microbiota of the nasal cavity. the purpose of this study was to determine the microbiota in different parts of the nasal cavity and to develop and evaluate methods for this purpose. samples were collected from 10 healthy adults by nasal washes and by swabbing of the mucosa through ... | 2000 | 11200821 |
analysis of bacterial dna in skin and muscle of the tyrolean iceman offers new insight into the mummification process. | about 80 sequences (16s ribosomal rna gene) of bacterial dna in samples of skin and muscle taken directly from the tyrolean iceman (3350-3100 years b.c.) or recovered during the 1992 archaeological expedition at the alpine site were analyzed to obtain clues to the natural mummification process that allowed the corpse of the neolithic shepherd/hunter to be preserved for more than 5,000 years. the investigation was made more complex by the fact that the surface of the mummy had been swabbed with p ... | 2000 | 10640948 |
purification and properties of pyridoxine oxidase from aureobacterium luteolum and pyridoxal reductase from schizosaccharomyces pombe. | 2000 | 10705982 | |
identification of coryneform bacterial isolates by ribosomal dna sequence analysis. | identification of coryneform bacteria to the species level is important in certain circumstances for differentiating contamination and/or colonization from infection, which influences decisions regarding clinical intervention. however, methods currently used in clinical microbiology laboratories for the species identification of coryneform bacteria are often inadequate. we evaluated the microseq 500 16s bacterial sequencing kit (perkin-elmer biosystems, foster city, calif.), which is designed to ... | 2000 | 10747168 |
high resistance to oxygen radicals and heat is caused by a galactoglycerolipid in microbacterium sp. m874. | microbacterium sp. m874 produced a glyceroglycolipid, di-o-12-methyl-tetradecanoyl-3-o-beta-d-galactopyranosyl-sn-glycerol, at about the 50 microm level. though the strain was highly resistant to tertiary-butyl hydroperoxide (tbhp) in a glycolipid-productive medium, the resistance was reduced in a nonproductive medium. exogenous addition of the glycolipid to the nonproductive culture restored the resistance. this addition also increased the resistance to heat, ethanol, and 4-chloro-1-naphthol, i ... | 2000 | 10788780 |
rescuing activity of galactoglycerolipids from cellular lesions induced by 5-aminolevulinic acid. | an anti-oxygen radical reagent of a bacterial metabolite, m874 monogalactoglycerolipid (di-o-12-methyl-tetradecanoyl-3-o-beta-d-galactopyranosyl-sn-glycerol ), was tested for its ability to protect two organisms against cellular lesions induced by 5-aminolevulinic acid (ala) and light. in corynebacterium flavescens atcc 10340, extracellular uroporphyrin and coproporphyrin were the main porphyrin products. although less than 2 mm ala increased porphyrin synthesis, ala levels above 3 mm inhibited ... | 2000 | 10788790 |
production and structure elucidation of glycoglycerolipids from a marine sponge-associated microbacterium species. | the bacterium microbacterium sp., isolated from the sponge halichondria panicea, produced four unusual cell-associated glycoglycerolipids and one diphosphatidylglycerol when grown on marine broth and on artificial seawater media. the lipids were isolated by chromatography on silica columns and their structures elucidated using a combination of multidimensional nmr and ms techniques. the main compound was 1-o-acyl-3-[alpha-glucopyranosyl-(1-3)-(6-o-acyl-alpha-mannopyranosyl )]glycerol (ggl.2) wit ... | 2000 | 10843572 |
comparison of colony lift with direct spotting methods of blot preparation on the effect of colony hybridization in the detection of environmental organisms. | nucleic acid probes are used on site to detect or to identify individual microbial cells without cultivation. this molecular technique can avoid some limitations of traditional identification methods including time consuming and imprecise. this study examined the factors affecting colony hybridization and compared the effectiveness of membrane prepared by colony lifting with direct spotting procedures using the universal probe eub 338. the results of hybridization varied depending on the type of ... | 2000 | 10917884 |
overexpression, purification, and characterization of bacillus subtilis n-acetylmuramoyl-l-alanine amidase cwlc. | n-acetylmuramoyl-l-alanine amidase cwlc of bacillus subtilis was overproduced in escherichia coli and purified 21-fold. the amidase hydrolyzed type a cell walls such as b. subtilis. the amidase bound slightly to the microbacterium lacticum cell wall (type b), but did not entirely hydrolyze it. the presence of calcium or magnesium ion increased the resistance of the amidase to heat denaturation. | 2000 | 10945275 |
microbial diversity during maturation and natural processing of coffee cherries of coffea arabica in brazil. | the magnitude and diversity of the microbial population associated with dry (natural) processing of coffee (coffea arabica) has been assessed during a 2-year period on 15 different farms in the sul de minas region of brazil. peptone water-washed samples were taken of maturing cherries on trees (cherries, raisins and dried cherries) and from ground fermentations. the microbial load varied from 3 x 10(4) to 2.2 x 10(9) cfu/cherry with a median value of 1.6 x 10(7) cfu/cherry. the microbial load in ... | 2000 | 11016614 |
identification of corynebacterium bovis and other coryneforms isolated from bovine mammary glands. | bovine mastitis remains the most economically important disease in dairy cows. corynebacterium bovis, a lipid-requiring corynebacterium spp., is frequently isolated from the milk of infected mammary glands of dairy cows and is associated with reduced milk production. a total of 212 coryneform bacteria isolated from the milk of dairy cows were obtained from mastitis reference laboratories in the united states and canada. all isolates had been presumptively identified as corynebacterium bovis base ... | 2000 | 11049082 |
a novel bacterial pathogen, microbacterium nematophilum, induces morphological change in the nematode c. elegans. | the dar (deformed anal region) phenotype, characterized by a distinctive swollen tail, was first detected in a variant strain of caenorhabditis elegans which appeared spontaneously in 1986 during routine genetic crosses [1,2]. dar isolates were initially analysed as morphological mutants, but we report here that two independent isolates carry an unusual bacterial infection different from those previously described [3], which is the cause of the dar phenotype. the infectious agent is a new specie ... | 2000 | 11137017 |
glyceroglycolipids preventing tert-butylhydroperoxide-induced cell death from microbacterium sp. and corynebacterium aquaticum strains. | galactosyl diacylglycerols m874b and s365b obtained from the recently isolated bacteria identified as microbacterium sp. m874 and corynebacterium aquaticum s365 were found to prevent oxidative cell death induced by tert-butylhydroperoxide. their structures were determined to be 1,2-di-o-(12-methyltetradecanoyl)-3-o-beta-d-galactopyranosyl-sn-glycerol and 1-o-(14-methylhexadecanoyl)-2-o-(12-methyltetradecanoyl)-3-o-beta-d-galactopyranosyl-sn-glycerol, respectively. | 2000 | 16232721 |
cloning of a novel prolidase gene from aureobacterium esteraromaticum. | the prolidase gene from aureobacterium esteraromaticum was cloned and expressed in escherichia coli. the cloned enzyme had the same enzymatic properties as the wild-type enzyme. kinetic analysis of the enzyme indicated that the best substrate was pro-hyp, which was not hydrolyzed by other prolidases. interestingly, there was no homology between the deduced amino acid sequence of a. esteraromaticum prolidase and those of the other sources such as human e. coli and lactobacillus. however, homology ... | 1999 | 9989239 |
application of temperature-gradient gel electrophoresis in taxonomy of coryneform bacteria. | strains belonging to the gram-positive coryneform soil bacteria were screened genotypically by temperature-gradient gel electrophoresis (tgge). this method allows the sequence-specific separation of amplified fragments of 16s rrna genes. a total of 115 reference strains representing the majority of the species of the genera aeromicrobium, agromyces, arthrobacter, aureobacterium, cellulomonas, curtobacterium, nocardioides and terrabacter were characterized. depending on the genus investigated, th ... | 1999 | 10028252 |
reclassification of brevibacterium oxydans (chatelain and second 1966) as microbacterium oxydans comb. nov. | phylogenetic and chemotaxonomic analyses indicate that brevibacterium oxydans is closely related to species of the genus microbacterium, namely microbacterium liquefaciens, microbacterium luteolum and microbacterium saperdae. dna-dna reassociation values of less than 60% between brevibacterium oxydans and these three microbacterium species support the distinctness of this misclassified brevibacterium species, which is reclassified as microbacterium oxydans comb. nov. | 1999 | 10028259 |
effect of elevated oxygen and carbon dioxide on the surface growth of vegetable-associated micro-organisms. | the impact of a novel type of modified atmosphere (ma), referred to as high o2-ma, on micro-organisms associated with the spoilage of minimally-processed vegetables was studied. pure cultures of pseudomonas fluorescens, enterobacter agglomerans, aureobacterium strain 27, candida guilliermondii, c. sake, salmonella typhimurium, salm. enteritidis, escherichia coli, listeria monocytogenes, leuconostoc mesenteroides var. mesenteroides, lactobacillus plantarum and lactococcus lactis were cultured on ... | 1999 | 10196748 |
actinomycetes in karstic caves of northern spain (altamira and tito bustillo). | a variety of isolation procedures were carried out to study the involvement of bacteria in the colonisation and biodeterioration of spanish caves with paleolithic rock art (altamira and tito bustillo). the applied techniques mainly aimed to isolate heterotrophic bacteria such as streptomycetes, nocardioform and coryneform actinomycetes, and other gram-positive and gram-negative bacteria. the results demonstrated that actinomycetes were the most abundant gram-positive bacteria in the caves. actin ... | 1999 | 10353805 |
analysis of the bacterial surface ripening flora of german and french smeared cheeses with respect to their anti-listerial potential. | the anti-listerial potential of 19 different french smeared cheese bacterial consortia was analyzed semi-quantitatively. comparison of the total viable cell count to the direct microscopic count yielded no indication that non-culturable bacteria contribute significantly to the undefined, complex ripening floras. from 2613 isolates, 48 showed clear inhibition of one or more listeria monocytogenes strains on solid medium, while only three isolates excreted an anti-listerial, soluble substance when ... | 1999 | 10357277 |
effects of combined shear and thermal forces on destruction of microbacterium lacticum. | a twin-screw extruder and a rotational rheometer were used to generate shear forces in concentrated gelatin inoculated with a heat-resistant isolate of a vegetative bacterial species, microbacterium lacticum. shear forces in the extruder were mainly controlled by varying the water feed rate. the water content of the extrudates changed between 19 and 45% (wet weight basis). higher shear forces generated at low water contents and the calculated die wall shear stress correlated strongly with bacter ... | 1999 | 10508076 |
aureobacterium masquerading as 'corynebacterium aquaticum' infection: case report and review of the literature. | a gram-positive bacillus was isolated repeatedly from blood taken through the lumina of a central venous catheter of a patient with multiple myeloma who developed febrile neutropenia following chemotherapy. the bacterium was identified by the api coryne system as 'corynebacterium aquaticum'. gene analysis targeting the 16s rrna indicated that the organism had a 99.5% identity with aureobacterium liquefaciens although there were two phenotypic characteristics at variance with the description of t ... | 1999 | 10510974 |
microbacterium kitamiense sp. nov., a new polysaccharide-producing bacterium isolated from the wastewater of a sugar-beet factory. | two strictly aerobic, heterotrophic and mesophilic new organisms, strains kitami a1 and kitami c2t, were isolated from the wastewater of a sugar-beet factory in kitami city, hokkaido, japan. in batch cultures, these organisms produced both insoluble and soluble exopolysaccharides (epss) utilizing sucrose as the sole carbon source. the g + c contents of the strains kitami c2t and kitami a1 were 69.2 mol%. both strains had anteiso-c15:0 acid, anteiso-c17:0 acid and iso-c16:0 as major components. t ... | 1999 | 10555312 |
facile enzymatic conversion of lactose into lacto-n-tetraose and lacto-n-neotetraose. | lacto-n-tetraose (galbeta1 -3glcnacbeta1-3galbeta1-4glc, lnt) and lacto-n-neotetraose (galbeta1-4glcnacbeta1-3galbeta1-4glc, lnnt) were enzymatically synthesized by consecutive additions of glcnac and gal residues to lactose. lacto-n-triose ii (glcnacbeta1-3galbeta1-4glc) was prepared first by the transfer of glcnac from udp-glcnac to lactose by beta-1,3-n-acetylglucosaminyltransferase from bovine serum. the resulting lacto-n-triose ii was converted into lnt and lnnt utilizing two kinds of beta- ... | 1999 | 10596893 |
psychrotrophic bacteria isolated from a constantly warm tropical environment. | psychrotrophic bacteria are known to occur in temperate, constantly cold, and artificially cooled environments. this is the first report of their occurrence in a constantly warm (ca. 24 degrees-35 degrees c) tropical environment. soil samples taken from two sites along the southeastern coastal zone of jamaica yielded growth of psychotrophic bacteria after 3-4 weeks of enrichment culture in 1/30 strength tryptic soy broth, 20 mg l-1 cycloheximide at 2 degrees c. growth of individual isolates at 2 ... | 1998 | 9516542 |
biodegradation of metal-edta complexes by an enriched microbial population. | a mixed culture utilizing edta as the sole carbon source was isolated from a mixed inoculum of water from the river mersey (united kingdom) and sludge from an industrial effluent treatment plant. fourteen component organisms were isolated from the culture, including representatives of the genera methylobacterium, variovorax, enterobacter, aureobacterium, and bacillus. the mixed culture biodegraded metal-edta complexes slowly; the biodegradability was in the order fe > cu > co > ni > cd. by incor ... | 1998 | 9546167 |
serum antibodies reacting with subgingival species in refractory periodontitis subjects. | the purpose of this investigation was to compare the levels of serum igg antibody to 85 subgingival species in 32 refractory periodontitis, 56 successfully treated, and 33 periodontally healthy subjects. refractory subjects showed mean full mouth attachment loss and/or >3 sites showing attachment loss >2.5 mm within 1 year after 2 treatment modalities, scaling and root planing and surgery plus systemically administered tetracycline. successfully-treated subjects showed mean attachment level gain ... | 1998 | 9696261 |
microbial communities of printing paper machines. | the microbial content of printing paper machines, running at a temperature of 45-50 degrees c and at ph 4.5-5, was studied. bacteria were prevalent colonizers of the machine wet end and the raw materials. a total of 390 strains of aerobic bacteria were isolated and 86% of these were identified to genus and species by biochemical, chemotaxonomic and phylogenetic methods. the most common bacteria found at the machine wet end were bacillus coagulans and other bacillus species, burkholderia cepacia, ... | 1998 | 9717292 |
union of the genera microbacterium orla-jensen and aureobacterium collins et al. in a redefined genus microbacterium. | the 16s rrna gene sequences of 19 strains, 11 strains representing validated aureobacterium or microbacterium species and eight strains of non-valid species or isolates, were determined. these sequences were aligned with the sequences of other validated aureobacterium and microbacterium species and related actinobacteria. a comparative sequence analysis of 43 strains revealed that the species of the genera aureobacterium and microbacterium form a monophyletic association in which species of both ... | 1998 | 9734028 |
proposal of six new species in the genus microbacterium and transfer of flavobacterium marinotypicum zobell and upham to the genus microbacterium as microbacterium maritypicum comb. nov. | reference strains, including two mis-named organisms, 'chromobacterium chocolatum' and flavobacterium marinotypicum, isolates from soil and clinical specimens, all previously recognized as aureobacterium or microbacterium, were characterized taxonomically. on the basis of morphological, physiological and chemotaxonomic characteristics, as well as dna-dna hybridization data, six new species and one new combination are proposed in the genus microbacterium: microbacterium ketosireducens sp. nov. (t ... | 1998 | 9734054 |
etherolytic cleavage of 4-(2,4-dichlorophenoxy)butyric acid and 4-(4-chloro-2-methylphenoxy)butyric acid by species of rhodococcus and aureobacterium isolated from an alkaline environment. | bacterial strains were isolated from the concrete rubble of a demolished herbicide production plant. the predominant feature of these strains was the etherolytic cleavage of 4-(2,4-dichlorophenoxy)butyric acid (dcpb)1) and 4-(4-chloro-2-methylphenoxy)butyric acid (mcpb) while liberating 2,4-dichlorophenol (dcp) and 4-chloro-2-methylphenol (mcp) respectively. some of the isolates were identified by 16s rdna sequence analysis and shown to belong to the genera aureobacterium sp. (strain k2-17) and ... | 1998 | 9867509 |
aureobacterium resistens sp. nov., exhibiting vancomycin resistance and teicoplanin susceptibility. | two similar strains of a coryneform bacterium were isolated from human clinical material. both strains were resistant to vancomycin but susceptible to teicoplanin. detailed biochemical, chemotaxonomical, and molecular genetic investigations revealed that both isolates were members of a hitherto undescribed species of genus aureobacterium. the name aureobacterium resistens sp. nov. is proposed for the new bacterium and the type strain is ccug 38312. | 1998 | 9453161 |
thermostable phenylalanine dehydrogenase from a mesophilic microbacterium sp. strain dm 86-1 | bacteria that produced nad+-dependent phenylalanine dehydrogenase (ec 1.4.1.20) were selected among l-methionine utilizers isolated from soil. a bacterial strain showing phenylalanine dehydrogenase activity was chosen and classified in the genus microbacterium. phenylalanine dehydrogenase was purified from the crude extract of microbacterium sp. strain dm 86-1 (tpu 3592) to homogeneity as judged by sds-polyacrylamide disc gel electrophoresis. the enzyme has an isoelectric point of 5.8 and a rela ... | 1998 | 9477256 |
[rapid diagnosis of tuberculosis using the amplification method]. | amplification methods identify microbacterium tuberculosis on the basis of genus- or species-specific sequence of bases in nucleic acids which they replete exponentially. the objective of the work was comparison of results of biological samples by the method gen-probe mycobacterium tuberculosis direct test (mtd) using amplification of ribosomal rna and bk by microscopy and cultivation, assessment of standard indicators of their efficiency of the method and analysis of diverging results. | 1997 | 9511260 |
clinical microbiology of coryneform bacteria. | coryneform bacteria are aerobically growing, asporogenous, non-partially-acid-fast, gram-positive rods of irregular morphology. within the last few years, there has been a massive increase in the number of publications related to all aspects of their clinical microbiology. clinical microbiologists are often confronted with making identifications within this heterogeneous group as well as with considerations of the clinical significance of such isolates. this review provides comprehensive informa ... | 1997 | 8993861 |
purification and characterization of dipeptidyl aminopeptidase from aureobacterium sp. wo26. | we isolated a bacterial strain with an enzyme which releases dipeptide from gly-arg-p-nitroanilide. the bacterium was tentatively identified as aureobacterium sp. the enzyme, named audap, was purified and characterized. it was homogenous by sds-page and ief, and had a molecular mass of 90,000 da by sds-page and 88,000 da by gel filtration, so it may be a monomer. the isoelectric point was 3.8 and the optimum ph was 10.0. the purified enzyme hydrolyzed gly-arg-pna, a model substrate for dap i, an ... | 1997 | 9028041 |
endophthalmitis due to microbacterium species: case report and review of microbacterium infections. | microbacterium species (formerly cdc [centers for disease control and prevention] coryneform group a-4 and a-5 bacteria) are widely distributed in the environment and rarely cause infections in humans. we present a case of endophthalmitis due to microbacterium species that occurred after accidental trauma and review the literature on microbacterium infections. if the infected tissue or medical device is removed and antimicrobial therapy (preferably with beta-lactams or glycopeptides) is institut ... | 1997 | 9145748 |
multicenter evaluation of the updated and extended api (rapid) coryne database 2.0. | in a multicenter study, 407 strains of coryneform bacteria were tested with the updated and extended api (rapid) coryne system with database 2.0 (biomérieux, la-balme-les-grottes, france) in order to evaluate the system's capability of identifying these bacteria. the design of the system was exactly the same as for the previous api (rapid) coryne strip with database 1.0, i.e., the 20 biochemical reactions covered were identical, but database 2.0 included both more taxa and additional differentia ... | 1997 | 9399506 |
activity of rifabutin, clarithromycin, ethambutol, sparfloxacin and amikacin, alone and in combination, against mycobacterium avium complex in human macrophages. | disseminated infection with microbacterium avium complex (mac) in patients with aids is currently treated with a combination of antimycobacterial agents in order to prevent the selection of resistant mutant strains. although clinical and microbiological responses can generally be achieved within a few weeks, relapses are common and require modification of the combination regimen or identification of effective alternate therapies. in this study we investigated the activities of rifabutin 0.5 mg/l ... | 1996 | 9182107 |
[microflora of the air in sawmills as a potential occupational hazard: concentration and composition of microflora and immunologic reactivity of workers to microbial aeroallergens]. | microbiologial studies of the air and allergological examinations of the workers were performed in two sawmills processing deciduous wood (mainly oak) and in one sawmill processing coniferous wood (mainly pine). the concentration of microorganisms in the air was of the order 10(3)-10(4) cfu/m3. the most common organisms were corynebacteria (arthrobacter, corynebacterium, brevibacterium, microbacterium), spore-forming bacilli (bacillus), gram-negative bacteria (rahnella) and filamentous fungi (as ... | 1996 | 9190234 |
[microflora of the in furniture factors as a potential occupational hazard: concentration and composition of microflora and immunologic reactivity of workers to microbial aeroallergens]. | microbiologial studies of the air were performed in two furniture factories. the concentration of microorganisms in the air was low, being of the order 10(3) cfu/m3. the most common organisms were corynebacteria (arthrobacter, corynebacterium, brevibacterium, microbacterium) and fungi (aspergillus fumigatus, rhodotorula rubra). some of the species found in this environment possess known allergenic properties. allergological examinations of the workers with environmental aeroallergens have been p ... | 1996 | 9190236 |
cloning and characterization of the mami restriction-modification system from microbacterium ammoniaphilum in escherichia coli. | the genes encoding a class-iin restriction-modification (r-m) system (mami, sequence specificity [symbol: see text] from microbacterium ammoniaphilum have been cloned in escherichia coli. the vector used for cloning was plasmid puc18 modified by the inclusion of three mami recognition sites. recombinant clones containing the mamim gene in its genomic context became fully methylated in vivo and remained completely resistant against digestion with the r.mami restriction endonuclease (enase). deter ... | 1996 | 8654988 |
characterization of bacterial communities from activated sludge: culture-dependent numerical identification versus in situ identification using group- and genus-specific rrna-targeted oligonucleotide probes | the structures of bacterial communities were studied in activated sludge samples obtained from the aerobic and anaerobic zones of a wastewater treatment plant showing enhanced phosphorous removal. samples were analyzed by in situ hybridization with oligonucleotide probes complementary to selected regions of the 16s and 23s ribosomal rna (rrna) characteristic for defined phylogenetic entities (genera and larger groups). the microbial community structures revealed by molecular techniques were comp ... | 1996 | 8688004 |
case of fatal systemic infection with an aureobacterium sp.: identification of isolate by 16s rrna gene analysis. | the case of a 75-year-old man who succumbed to a disseminated infection most likely caused by a species of the genus aureobacterium is reported. identification of the isolate was achieved by comparative 16s rrna gene analysis. aureobacteria are commonly found in the environment. however, only recently have they been recognized as a cause of infections including septicemia and soft tissue infections. to our knowledge, this is the first documentation of a fatal infection caused by an aureobacteriu ... | 1996 | 8735113 |
purification and characterization of a prolidase from aureobacterium esteraromaticum. | an edta-insensitive prolidase (proline dipeptidase, ec 3.4.13.9) was isolated from a cell-free extract of aureobacterium esteraromaticum ifo 3752. the enzyme was purified almost to homogeneity using acetone precipitation, hydrophobic chromatography, ion-exchange chromatography, and gel-permeation chromatography. the enzyme has a molecular weight of about 440,000 by gel permeation chromatography, and about 40,000 by sds polyacrylamide gel electrophoresis. the isoelectric point was 4.6. the enzyme ... | 1996 | 8782407 |
bioavailability of selenium accumulated by selenite-reducing bacteria. | the bioavailability of selenium (se) was determined in bacterial strains that reduce selenite to red elemental se (seo). a laboratory strain of bacillus subtilis and a bacterial rod isolated from soil in the vicinity of the kesterson reservoir, san joaquin valley, ca, (microbacterium arborescens) were cultured in the presence of 1 mm sodium selenite (na2seo3). after harvest, the washed, lyophilized b. subtilis and m. arborescens samples contained 2.62 and 4.23% total se, respectively, which was ... | 1996 | 8811279 |
vancomycin-resistant aureobacterium species cellulitis and bacteremia in a patient with acute myelogenous leukemia. | a 39-year-old male with acute myelogenous leukemia and concomitant porphyria cutanea tarda was admitted to the hospital for consolidation chemotherapy of his leukemia. during his hospitalization, he developed cellulitis of the left hand and persistent bacteremia with a yellow-pigmented, nonfermenting coryneform bacterium that was identified as aureobacterium sp. the portal of entry for the aureobacterium infection was probably through the skin lesions due to porphyria cutanea tarda. the infectio ... | 1996 | 8818896 |
an identification scheme for rapidly and aerobically growing gram-positive rods. | an identification scheme for aerobically growing gram-positive rods (genera actinomyces, arcanobacterium, aureobacterium, bacillus, brevibacterium, cellulomonas, corynebacterium, dermabacter, erysipelothrix, gardnerella, lactobacillus, listeria, microbacterium, oerskovia, propionibacterium, rhodococcus, rothia, turicella, as well as unnamed cdc groups, clostridium tertium, and mycobacterium fortuitum/chelonae) is presented. it is derived from the hollis-weaver scheme and uses catalase, oxidative ... | 1996 | 8837385 |
the application of serological techniques to the taxonomy of arthrobacter and related organisms. | antisera were raised against rods of 17 named arthrobacter and aureobacterium strains. antigenic relationships between these strains, other soil bacteria and new arthrobacter isolates from several soils were studied, using agglutination, immunodiffusion, immunofluorescence and elisa techniques. many of the named arthrobacter species had common antigens, and there were also common antigens amongst named arthrobacter strains and many fresh arthrobacter isolates. agglutination, elisa and immunofluo ... | 1996 | 8868431 |
degradation of prochloraz and 2,4,6-trichlorophenol by environmental bacterial strains. | eight bacterial isolates from enrichment with 2,4,6-trichlorophenol (tcp) as sole carbon source were tested for their potential to degrade prochloraz. none of them could grow on prochloraz. strain c964, identified as aureobacterium sp., effectively reduced the fungitoxic activity of prochloraz in a bioassay and degradation was confirmed by hplc. two other isolates, strain c611 and c961, using tcp as a carbon source, belong to the beta subclass of the proteobacteria and presumely degrade tcp via ... | 1996 | 8920198 |
[hypersensitivity pneumonitis caused by a home humidifier]. | a 33-year-old man was admitted complaining of a fever, dyspnea, and a dry cough almost every night since december of 1992. he had been using an ultrasonic humidifier at home. the chest ct scan and roentgenogram showed bilateral reticulonodular shadows. after admission, the symptoms resolved spontaneously. these findings were suggestive of hypersensitivity pneumonitis. after analysis of fluid obtained by bronchoalveolar lavage and of a specimen obtained by transbronchial biopsy, "humidifier lung" ... | 1995 | 8538084 |
primary identification of microbacterium spp. encountered in clinical specimens as cdc coryneform group a-4 and a-5 bacteria. | over nearly two decades, 13 yellow- or orange-pigmented, fermentative gram-positive rods belonging to the genus microbacterium were encountered in clinical specimens. all 13 strains, 10 of which came from blood cultures, were initially identified as cdc coryneform group a-4 and a-5 bacteria according to the scheme of hollis and weaver for the identification of gram-positive rods. the clinical isolates were compared with the type strains of the six species constituting the genus microbacterium as ... | 1995 | 7699039 |
primary identification of aureobacterium spp. isolated from clinical specimens as "corynebacterium aquaticum". | over a 6-year period 11 yellow-pigmented gram-positive rods (gprs) with an oxidative carbohydrate metabolism were isolated from clinical specimens or were received as reference cultures and tentatively identified as "corynebacterium aquaticum" according to the guide of hollis and weaver for the differentiation of gprs (d. g. hollis and r. e. weaver, gram-positive organisms: a guide to identification, 1981). because these isolates seemed to be rather heterogeneous, comparative analyses with the t ... | 1994 | 7852557 |
an outbreak of ochrobactrum anthropi bacteraemia in five organ transplant patients. | nosocomial bacteraemia caused by ochrobactrum anthropi occurred over a 1-month period in five organ transplant recipients, four of whom were in the same renal and pancreatic transplant unit. bacteraemia occurred with cyclosporin a, azathioprine and steroids, and with a rabbit anti-thymocyte globulin (ratg) during the induction phase. ratg appeared to be the only common factor among the five cases. over the period described, 71.4% of all patients receiving ratg developed o. anthropi bacteraemia. ... | 1994 | 7916361 |
in situ probing of gram-positive bacteria with high dna g + c content using 23s rrna-targeted oligonucleotides. | 23s-rrna-targeted oligonucleotide probes were designed for the phylogenetic group 'gram-positive bacteria with high g + c content of dna' (gpbhgc). a sequence idiosyncrasy in two adjacent base pairs in the stem of helix 69 in domain iv of the 23s rrna is present in all hitherto analysed strains of gpbhgc. an oligonucleotide probe targeted to this region hybridized only with strains of gpbhgc and was successfully used for in situ monitoring of these cells in activated sludge. another unique featu ... | 1994 | 8000548 |
phylogenetic analysis of the genus microbacterium based on 16s rrna gene sequences. | 16s rrna gene (rdna) studies of the six species of the genus microbacterium, m. lacticum, m. laevaniformans, m. dextranolyticum, m. imperiale, m. arborescens and m. aurum, were performed and the primary structures were compared with those of 29 representative actinobacteria and related organisms. phylogenetic analysis indicated that six species of the genus microbacterium and representative four species of the genus aureobacterium appear to be phylogenetically coherent as was suggested by rainey ... | 1994 | 7528152 |
suprageneric classification of peptidoglycan group b actinomycetes by nucleotide sequencing of 5s ribosomal rna. | 5s ribosomal rna sequences were determined for thirteen actinomycetes mainly representatives with the rare group b type peptidoglycan. the primary and secondary structure of the resultant sequences were of the type characteristic of gram-positive bacteria with dna rich in guanine plus cytosine. the sequencing and associated chemotaxonomic data provide compelling grounds for classifying actinomycetes with a group b type peptidoglycan in a single family. the family microbacteriaceae fam. nov. is p ... | 1993 | 8085792 |
proposal of six new species in the genus aureobacterium and transfer of flavobacterium esteraromaticum omelianski to the genus aureobacterium as aureobacterium esteraromaticum comb. nov. | twelve strains placed in the genera flavobacterium, pseudomonas, and aureobacterium, including soil isolates, were characterized taxonomically. on the basis of morphological, physiological, and chemotaxonomic data, as well as dna-dna hybridization data, we propose that 11 of these strains should be classified in the genus aureobacterium as new combinations or new species, as follows: aureobacterium esteraromaticum comb. nov. (type strain, ifo 3751 [= atcc 8091]), aureobacterium arabinogalactanol ... | 1993 | 8347513 |
polyphosphate-dependent enzymes in some coryneform bacteria isolated from sewage sludge. | eleven isolates obtained from a laboratory sewage treatment plant, most of them presumptively assigned to the coryneform genera curtobacterium and aureobacterium were studied for the presence of intracellular polyphosphates and polyphosphate dependent enzymes. all isolates stored polyphosphates and showed adenylate kinase activities ranging from 64 to 815 mu mg-1. polyphosphate:amp phosphotransferase could only be detected in one isolate. three isolates showed a polyphosphate kinase activity als ... | 1993 | 8386121 |
chemotaxonomy and molecular taxonomy of some coryneform clinical isolates. | six reference strains of the genus brevibacterium as well as fifteen clinical isolates tentatively assigned to the genus using conventional biochemical methods, were the subject of chemotaxonomic and dna similarity studies. five of these clinical isolates were assigned either to the genera aureobacterium, mycobacterium, gordona or to rhodococcus on the basis of their dna mol% g+c, mycolic acid, amino-acid, sugar and menaquinone contents. among the ten remaining strains, six were not brevibacteri ... | 1993 | 8518510 |
formation of beta-fructosyl compounds of pyridoxine in growing culture of aspergillus niger. | two pyridoxine compounds were found to be formed in a culture filtrate of aspergillus niger and a. sydowi, when grown in a medium containing sucrose and pyridoxine. each of the two compounds i and ii was obtained as a white powdered preparation by preparative paper chromatography, gel filtration on toyopearl hw-40s and sephadex g-10 columns, deae-cellulose column chromatography, and lyophilization. compounds i and ii were identified as 5'-o-(beta-d-fructofuranosyl)-pyridoxine and 5'-o-[beta-d-fr ... | 1993 | 7763873 |
culture media for non-sporulating gram-positive food spoilage bacteria. | the spoilage association especially of protein-rich foods can be dominated by gram-positive bacteria, notably lactic acid bacteria (lab) which affect vacuum packaged refrigerated processed meats and some dairy products. new food ecosystems are being created by novel packaging and processing technologies, resulting in spoilage associations differing from those previously reported. in addition, improvement in identification methods, allow the detection and isolation of 'novel' bacterial groups, e. ... | 1992 | 1486021 |
aspei, a novel eam11051 isoschizomer from aureobacterium species recognizing 5'-gacnnn/nngtc-3'. | 1992 | 1641345 | |
chiral linear hydroxamates as biomimetic analogues of ferrioxamine and coprogen and their use in probing siderophore-receptor specificity in bacteria and fungi. | linear hydroxamate derivatives, possessing chiral alpha-amino acid moieties, were synthesized and their iron transport activities were studied in bacteria and fungi. no growth-promoting activity could be detected in the gram-positive hydroxamate-auxotroph aureobacterium flavescens jg9. however, gram-negative enterobacteria, such as escherichia coli, pantoea agglomerans and hafnia alvei were able to utilize iron from these analogues. uptake of 55fe-labeled analogues was inhibited by sodium azide, ... | 1991 | 1657086 |
the specificity of bacterial siderophore receptors probed by bioassays. | the ability to utilize siderophores of bacterial and fungal origin has been studied in wild-type and mutant strains of the enterobacterial genera salmonella, escherichia, shigella, moellerella, klebsiella, enterobacter, hafnia, pantoea, ewingella, tatumella, yersinia, and in the non-enterics aeromonas, pseudomonas and aureobacterium. although only a few representative strains were tested, the results show characteristic genus-specific differences in the utilization of hydroxamate and catecholate ... | 1991 | 1663779 |
microbial metabolism of quinoline and related compounds. xi. degradation of quinoline-4-carboxylic acid by microbacterium sp. h2, agrobacterium sp. 1b and pimelobacter simplex 4b and 5b. | from soil enrichment cultures four strains, using quinoline-4-carboxylic acid as sole source of energy and carbon, have been isolated. according to their physiological properties these bacteria have been identified as microbacterium sp. designated h2, as agrobacterium sp. designated 1b and pimelobacter simplex designated 4b and 5b. metabolites of the degradation pathway of quinoline-4-carboxylic acid have been isolated and identified. with pimelobacter simplex 4b and 5b 2-oxo-1,2-dihydroquinolin ... | 1991 | 1793516 |
transfructosylation of rebaudioside a (a sweet glycoside of stevia leaves) with microbacterium beta-fructofuranosidase. | it was found that a beta-fructofuranosidase produced by microbacterium sp. h-1 has potent trans-beta-fructofuranosylation activity from sucrose (donor). by means of this enzyme system, rebaudioside a (ra), the second major sweet steviol glycoside of the leaves of stevia rebaudiana, was subjected to transfructosylation, affording a mono-beta-fructofuranosylated product (ra-f) in a high yield. the structure of ra-f was elucidated as beta-d-fructofuranosyl-(2----6)-beta-d-glucopyranosyl ester of st ... | 1991 | 1797424 |