Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| [sequence analysis of the matrix protein and fusion protein genes of a field peste des petits ruminants virus strain from tibet, china]. | the nucleotide sequences of m and f genes from a field strain of peste des petits ruminants virus (pprv) ("china/tib/gej/07-30") was firstly determined. the m gene was 1 483 nucleotides in length with a single open reading frame (orf), encoding a protein of 335 amino acids. the f gene was 2411 nucleotides in length, encoding a protein of 546 amino acids. the resulting nucleotide sequence and the deduced amino acid sequences were compared with the homologous regions of other pprv isolates. the nu ... | 2010 | 20836385 |
| [construction and sequencing of full-length cdna of peste des petits ruminants virus]. | to develop a reverse genetics system of peste des petits ruminants virus(pprv), five pairs of oligonucleotide primers were designed on the basis of the full-length genomic sequence of pprv nigeria 75/ 1 strain. using rt-pcr technique, five over-lapping cdna fragments, designated as jf1, jf2, jf3, jf4 and jf5, respectively, were amplified, followed by cloning into pcdna3.1(+)vector. an asci restriction enzyme site and a t7 promoter sequence were introduced immediately upstream of 5'-end, while a ... | 2010 | 20836386 |
| [genome sequencing and analysis of a peste des petits ruminants virus isolate, china/tib/07]. | peste des petits ruminants virus is a member of morbillivirus paramyxoviridae. the complete genome of a peste des petits ruminants virus (pprv) isolate, china/tib/07 was sequenced and molecular characteristics was analyzed. the internal sequences of the virus genome were amplified by rt-pcr with primers designed according to the published data in genbank, while the sequences of the 3' and 5' ends of the genome were determined by race. amplification products were directly sequenced,assembled and ... | 2010 | 20836387 |
| isolation of bluetongue virus serotype 1 (btv-1) from goats and its phylogenetic relationship to other btv-1 isolates worldwide based on full-length sequence of genome segment-2. | eight bluetongue viruses (btv) were isolated in bhk-21 cell culture from blood of goats suffering from peste des petits ruminants. these viruses were identified as btv serotype 1 (btv-1) by rt-pcr using vp2-gene-based primers coupled with sequencing of the pcr products. all of the isolates showed similar genome migration profile in 8% polyacrylamide gel electrophoresis. the genome segment-2 (seg-2) of one isolate (mkd18/india/2008) was amplified piecemeal by overlapping pcr, and the products wer ... | 2010 | 20842392 |
| comparative efficacy of peste des petits ruminants (ppr) vaccines. | peste des petits ruminants (ppr) is a highly contagious, economically important viral disease of sheep and goats with high morbidity and mortality rates. in order to control the disease effectively, highly sensitive diagnostic tests coupled with potent vaccines are important pre-requisites. at present, there are three live attenuated ppr vaccines available in india including sungri 96, arasur 87 and coimbatore 97. indian veterinary research institute (ivri) mukteswar developed the ppr sungri 96 ... | 2010 | 20199873 |
| isolation and identification of virulent peste des petits ruminants viruses from ppr outbreaks in india. | in this study, three outbreaks of peste des petits ruminants (ppr) in goats and sheep flocks with high morbidity and considerable mortality were recorded at jhansi and revati in uttar pradesh and bhopal in madhya pradesh, india during 2003-2006. clinical samples were collected from the affected flocks for laboratory investigation. the ppr virus (pprv) antigen/nucleic acid in the infected tissues/swab materials was demonstrated by using sandwich enzyme-linked immunosorbent assay (elisa) and rever ... | 2010 | 20204510 |
| peste des petits ruminants in arabian wildlife. | recurrence of peste des petits ruminants (ppr) was diagnosed in the united arabian emirates in several wild ruminants confirmed by morphological, immunohistochemical, serological and molecular findings. phylogenetic analysis revealed that the virus strain belongs to lineage iv, which is different to some previously isolated ppr strains from the arabian peninsula. this study shows that wild ruminants may play an important epidemiological role as virus source for domestic small ruminants. | 2010 | 20067659 |
| livestock pathology in the central african region: some epidemiological considerations and control strategies. | disease consistently features among the major constraints of livestock production in the central african region, orchestrating important economic losses. this article reviews livestock diseases of economic importance, including foot-and-mouth disease, trypanosomosis and dermatophilosis in cattle, peste des petits ruminants and gastrointestinal helminthosis in sheep and goats, and newcastle disease in poultry. some aspects of epidemiology such as pathogen identification, prevalence and risk facto ... | 2010 | 20074399 |
| agricultural diseases on the move early in the third millennium. | with few exceptions, the diseases that present the greatest risk to food animal production have been largely similar throughout the modern era of veterinary medicine. the current trend regarding the ever-increasing globalization of the trade of animals and animal products ensures that agricultural diseases will continue to follow legal and illegal trade patterns with increasing rapidity. global climate changes have already had profound effects on the distribution of animal diseases, and it is an ... | 2010 | 20080480 |
| experimental infection of peste des petit ruminant virus and mannheimia haemolytica a2 in goats: immunolocalisation of mannheimia haemolytica antigens. | nigerian strain of peste des petit ruminant (ppr) virus and mannheimia haemolytica (mh) biotype a serotype 2, was used successfully to reproduce a concurrent disease in west african dwarf goats. the development of the various pathological features were studied at regular intervals following infection. the acute inflammatory reaction which had developed by day 3 after initial infection was characterised by flooding of the alveoli by neutrophils, oedema, hemorrhage and syncytial cells together wit ... | 2010 | 20617380 |
| application of semi-quantitative m gene-based hydrolysis probe (taqman) real-time rt-pcr assay for the detection of peste des petits ruminants virus in the clinical samples for investigation into clinical prevalence of disease. | peste des petits ruminants (ppr) is a contagious, notifiable and economically important transboundary viral disease of small ruminants. in this study, a hydrolysis probe-based real-time reverse transcription-polymerase chain reaction (rt rt-pcr) assay for the detection and semi-quantification of ppr virus (pprv) nucleic acid was developed using the virus rna and matrix (m) gene-specific primers with hex-labelled fluorescent probe and applied for the detection of pprv in clinical samples to ident ... | 2010 | 20723163 |
| current situation of peste des petits ruminants (ppr) in the sudan. | the current situation of ppr in sudan was investigated. a total of 61 tissue samples were collected from various ppr suspected outbreaks in sheep in sudan during 2008. collected tissue samples were tested for ppr antigen using icelisa, ppr antigen was detected in 26 out of 61 samples (42.6%). highest antigen detection rate was in specimens collected from western sudan. a total of 1198 serum samples were collected from sheep (n = 500), camels (n = 392), and goats (n = 306) from different areas in ... | 2010 | 19548103 |
| a serologic investigation for peste des petits ruminants infection in sheep, cattle and camels (camelus dromedarius) in aydin province, west anatolia. | 2010 | 19554466 | |
| [construction of an infectious clone of pseudorabies virus strain zj genome maintained as a bacterial artificial chromosome]. | pha2 plasmid sequence,with bacterial artificial chromosome(bac) vector and the gfp expression cassette, was introduced into the ul23(tk) gene of pseudorabies virus(prv)strain zj by homologous recombination,and the recombinant prv (rprv-ha2) was confirmed and isolated by plaque purification. the circular genome of rprv-ha2 was electroporated into escherichia coli strain dh10b and then the prv bac (pprv) was recovered. the transfection of pprv into veroe6 cells resulted in productive infection. th ... | 2010 | 20836388 |
| virological and antigenic characterization of two peste des petits ruminants (ppr) vaccine viruses of indian origin. | virological and antigenic characteristics of two indian peste des petits ruminants (ppr) vaccine viruses namely "pprv-sungri/96" and "pprv-ar/87" were investigated. this investigation included, type of cytopathic effect (cpe) produced, one-step growth curve, antigenic reactivity of viral antigens using a panel of 18 monoclonal antibodies (mabs) and ability of viruses for neutralization using a monoclonal antibody directed to haemagglutinin (h) protein. findings surprisingly indicated that the pp ... | 2010 | 19200598 |
| mva recombinants expressing the fusion and hemagglutinin genes of pprv protects goats against virulent challenge. | peste des petits ruminants (ppr) is a highly contagious animal disease caused by the peste des petits ruminants virus (pprv) belonging to the genus morbillivirus and family paramyxoviridae. the disease results in high morbidity and mortality in goats, sheep and in some small wild ruminants. the presence of large number of small ruminants reared in endemic areas makes ppr a notorious disease threatening the livelihood of poor farmers. conventional vaccination using a live, attenuated vaccine give ... | 2010 | 23100840 |
| rapid detection of peste des petits ruminants virus by a reverse transcription loop-mediated isothermal amplification assay. | peste des petits ruminants virus (pprv) is the causative agent of peste des petits ruminants (ppr), an economically important viral disease of small ruminants. in this report, a one-step, single-tube, reverse transcription loop-mediated isothermal amplification (rt-lamp) assay was developed for the detection of pprv. a set of six lamp primers were designed based on the matrix gene sequence of pprv to amplify the target rna by incubation at 63°c for 60min with bst dna polymerase and reverse trans ... | 2010 | 20813134 |
| quantitative one-step real-time rt-pcr for the fast detection of the four genotypes of pprv. | a one-step real-time taqman rt-pcr assay (rrt-pcr) for peste des petits ruminants virus (pprv) was developed to detect the four lineages of pprv by targeting the nucleoprotein (n) gene of the virus. this new assay was compared to a conventional rt-pcr on reference strains and field materials. quantitation was performed against a standard based on a synthetic transcript of the nppr gene for which a minimum of 32 copies per reaction were detected with a corresponding c(t) value of 39. depending on ... | 2010 | 20116402 |
| effects of dietary chromium supplementation on glucose tolerance and primary antibody response against peste des petits ruminants in dwarf bengal goats ( capra hircus). | an experiment was conducted to exploit the immune-modulation effect of trivalent chromium (cr) for augmenting immunity against peste des petits ruminants (ppr) in dwarf black bengal goats (capra hircus). the goats (n = 6 per treatment group) either received a basal diet devoid of supplemental cr (control) or were supplemented with 0.5 mg cr as chromic chloride (+cr) for 60 days. live weight gain and feed conversion efficiency improved (p < 0.05) in the +cr dietary group. supplemental cr did not ... | 2009 | 22444223 |
| expression of peste des petits ruminants virus nucleocapsid protein in prokaryotic system and its potential use as a diagnostic antigen or immunogen. | in this study, both partial and full-length nucleocapsid (n) gene of peste des petits ruminants virus (pprv) were cloned into pet33b vector and expressed in escherichia coli (bl21) with the objective of replacing live pprv antigen with recombinant protein in elisa. the expressed proteins were characterized by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and western blot by using a pprv n protein specific monoclonal antibody. the expressed histidine-tagged fusion proteins were purif ... | 2009 | 19646481 |
| prevalence and distribution of peste des petits ruminants virus antibodies in various districts of tanzania. | despite the widespread prevalence of infection with peste des petits ruminants virus (pprv) in goats and sheep industry in asia and sub-saharan africa, there have been few, if any, structured population-based studies examining the epidemiology of this infection in tanzania. in this study, we investigated the seroprevalence, and risk factors, of peste des petitis ruminants(ppr) in sheep and goat flocks from seven different geographical administration authorities (ngorongoro, monduli, longido, kar ... | 2009 | 19705291 |
| antibody seroprevalences against peste des petits ruminants (ppr) virus in sheep and goats in sudan. | counter immnuo-electrophoresis (ciep) and competitive elisa (c-elisa) tests were employed for seroprevalence of peste des petits ruminants (ppr) infection in sudan. the result of both tests showed high prevalence of pprv antibodies in sheep and goats sera collected from six different regions of sudan. of the 519 serum samples examined for the presence of pprv antibodies 307(59.15%) were positive by ciep while 263(50.67%) were positive by c-elisa. ciep technique was shown to be more sensitive tha ... | 2009 | 19798772 |
| the early detection of peste-des-petits-ruminants (ppr) virus antigens and nucleic acid from experimentally infected goats using rt-pcr and immunocapture elisa techniques. | goats were infected subcutaneously with different african and indian isolates of peste-des-petits-ruminants virus. typical signs of disease were recorded from day 6 post infection for all isolates. ocular, nasal and mouth samples were tested for the presence of virus antigen or nucleic acid using the immunocapture elisa (ice) and the rt-pcr technique. using ice, virus antigen was detected at day 4 in ocular and nasal samples of goats infected with côte-d'ivoire 89 and in the ocular, nasal and mo ... | 2009 | 19339027 |
| a vero cell derived combined vaccine against sheep pox and peste des petits ruminants for sheep. | the combined sheep pox and peste des petits ruminants (ppr) vaccine was prepared in lyophilized form containing recommended doses of both vaccine viruses. safety and immunogenicity of this combined vaccine was evaluated in sheep. sheep immunized subcutaneously with 1ml of live attenuated vaccine consisting of 10(3)tcid(50) each of sheep pox virus (spv) romanian fanar (rf) strain and peste des petits ruminants virus (pprv-sungri/96 strain) were monitored for clinical and serological responses for ... | 2009 | 19428860 |
| peste des petits ruminants virus in tibet, china. | serologic and molecular evidence indicates that peste des petits ruminants virus (pprv) infection has emerged in goats and sheep in the ngari region of southwestern tibet, people's republic of china. phylogenetic analysis confirms that the pprv strain from tibet is classified as lineage 4 and is closely related to viruses currently circulating in neighboring countries of southern asia. | 2009 | 19193278 |
| [recombinant goat pox virus expressing pprv h protein]. | the purpose of the study is to construct recombinant goat pox virus (gpv) expressing peste des petits ruminants virus (pprv) h protein, and to evaluate the immunization effect. recombinant gpv containing pprv h gene (rgpv-pprv-h) was selected and purified by gpt and egfp utilizing plaque purification, and the final selected recombinant gpv was proved to be purified by pcr. immunofluorescence and western blotting showed that the recombinant virus could express h protein of pprv while infecting la ... | 2009 | 19637621 |
| evidence of mixed infection of peste des petits ruminants virus and bluetongue virus in a flock of goats as confirmed by detection of antigen, antibody and nucleic acid of both the viruses. | a mixed infection with peste des petits ruminants virus (pprv) and bluetongue virus (btv) occurred in goats which exhibited symptoms characteristic of ppr. a number of samples were collected from ailing or dead goats for labrotory diagnosis. antibody to btv and pprv was detected in sera samples by competitive elisa. no pprv antigen was detected in tissue samples like lung and spleen, however, presence of pprv antigen in some sera samples was confirmed by sandwich elisa. all the blood samples col ... | 2009 | 19430955 |
| synthesis and antiviral activity of 4,4'-(arylmethylene)bis(1h-pyrazol-5-ols) against peste des petits ruminant virus (pprv). | an efficient and eco-friendly method for the synthesis of 4,4'-(arylmethylene)bis(1h-pyrazol-5-ols) has been accomplished by tandem knoevenagel-michael reaction of two equivalents of 5-methyl-2-phenyl-2,4-dihydro-3h-pyrazol-3-one with various aromatic aldehydes catalyzed by ceric ammonium nitrate (can) in water. all the synthesized compounds 3a-j were evaluated for in vitro antiviral activity against peste des petits ruminant virus (pprv). compound 3i emerged as the most interesting compound in ... | 2009 | 19482473 |
| using participatory epidemiological techniques to estimate the relative incidence and impact on livelihoods of livestock diseases amongst nomadic pastoralists in turkana south district, kenya. | a participatory epidemiological (pe) study was carried out with turkana pastoralists in turkana south district, kenya, to determine the relative incidence of livestock diseases and their impact on livelihoods. a sub-location was used as the sampling unit. a sub-location is the smallest administrative unit and is occupied by clusters of families (called adakars) that share common grazing patterns. a total of 32 sub-locations were randomly selected for the study. at least one focus group discussio ... | 2009 | 19487039 |
| sequence analysis of the large (l) polymerase gene and trailer of the peste des petits ruminants virus vaccine strain nigeria 75/1: expression and use of the l protein in reverse genetics. | the large (l) polymerase gene and the 5'-terminal utr of the genome of peste des petits ruminants virus (pprv), vaccine strain nigeria 75/1, were cloned and sequenced. the l protein was also expressed in eukaryotic cells and its polymerase activity was quantitatively measured in a ppr reverse genetics assay using a reporter minigenome. comparative sequence analysis of this functional l gene with corresponding genes of other morbilliviruses showed a degree of conservation exceeding 70%. the multi ... | 2009 | 19540279 |
| peste des petitis ruminants morbillivirus infection in lambs and young goats at qassim region, saudi arabia. | an outbreak of peste des petits ruminants (ppr) in lambs and young goats of najdi breed of sheep and goats occurred during winter 2005 at qassim region of central saudi arabia. the ppr infection was confirmed by demonstration of antibodies against the virus in the serum of clinically-ill young sheep and goats using competitive elisa test. clinical examination of infected animals showed fever, salivation, lacrimation, mucopurulent nasal discharge, difficult breathing and diarrhoea. the disease wa ... | 2009 | 18504645 |
| comparative efficacy of standard agid and precipitinogen inhibition test with monoclonal antibodies based competitive elisa for the serology of peste des petits ruminants in sheep and goats. | this project was conducted to investigate the comparative efficiency of competitive elisa (celisa), standard agar gel immunodiffusion test (agid) and precipitinogen inhibition test (pit) for the diagnosis of peste des petits ruminants (ppr) in pakistan. to deal with this, serum samples from 198 sheep and 82 goats were collected from three different government livestock farms and all the samples were run simultaneously with the three serological tests. the samples found positive for ppr antibodie ... | 2009 | 18622735 |
| ppr virus infection on sheep in blacksea region of turkey: epidemiology and diagnosis by rt-pcr and virus isolation. | in this study, a totally 164 materials (lung, spleen, lymph node, nasal and ocular swap, blood and samples from oral lesions) from sheep and lambs (n = 57) in the 34 flocks suspected the pprv infection as clinically and macroscopic pathologic remarks, housed in the 4 different provinces in the middle and eastern blacksea region were used for rt-pcr and virus isolation. additionally, serum samples randomly collected from 892 sheep were tested for the detection of pprv seroprevalance in the same r ... | 2009 | 18787968 |
| utilization of protein-a in immuno-histochemical techniques for detection of peste des petits ruminants (ppr) virus antigens in tissues of experimentally infected goats. | this paper constitutes the first record of utilizing the s. aureus protein-a (pa), conjugated to peroxidase enzyme, for the detection of the peste des petits ruminants (ppr) virus antigens in tissues of experimentally infected goats. the goats were experimentally infected with a virulent ppr virus, which was previously isolated from a severe natural disease outbreak in gazelles, during 2002 in saudi arabia. the technique is rapid, and has the superiority over the peroxidase -anti-peroxidase (pap ... | 2009 | 19052895 |
| pathological, serological, and virological findings in sheep infected simultaneously with bluetongue, peste-des-petits-ruminants, and sheeppox viruses. | in this study, pathological, serological and virological examinations were performed on 15 sheep from a flock of 250 sheep and lambs that suffer from simultaneous naturally occurring btv, pprv and spv outbreaks. spv was diagnosed macroscopically and histopathologically, btv was diagnosed by elisa, and pprv was diagnosed pathologically and by elisa. clinically fever, diarrhea, depression, polypnea, conjunctivitis, lacrimation, rhinitis, erosive stomatitis, edema of eyelids, photophobia, cutaneous ... | 2009 | 19067219 |
| real-time rt-pcr assays for the rapid and differential detection of dolphin and porpoise morbilliviruses. | real-time rt-pcr (rtrt-pcr) assays for identifying and differentiating infections caused by dolphin morbillivirus (dmv) and porpoise morbillivirus (pmv) were developed by targeting the hypervariable c-terminal domain of the nucleocapsid (n) gene. total dmv and pmv rna extracted from infected vero cells expressing the canine signaling lymphocyte-activation molecule (slam) produced positive cycle threshold (c(t)) values after the 17th and 25th cycles, respectively. the assays were then validated u ... | 2009 | 19084557 |
| post-vaccination antibodies profile against peste des petits ruminants (ppr) virus in sheep and goats of punjab, pakistan. | a total of 70 sheep and 330 goats were selected randomly. all the animals were kept under same housing and management conditions. serum samples were collected from all the animals and tested for the presence of antibodies against peste des petits ruminants (ppr) virus using competitive elisa (celisa). all the animals were found negative showing percentage inhibition (pi) values <50. the animals were vaccinated against ppr with nig/75/1 strain vaccine of ppr serum samples were collected from rand ... | 2009 | 18758987 |
| peste des petits ruminants virus (pprv) infection; its association with species, seasonal variations and geography. | the present investigation included a detailed description of the factors directly associated with pprv infection in pakistan. a total of 1,056 suspected serum samples were analyzed for the presence of antibodies to pprv with no history of vaccination against ppr. the samples were collected from sixty two (62) suspected outbreaks from twenty five (25) major regions of the country. samples were collected from the animals suffering from diarrhea and showing severe respiratory signs. competitive enz ... | 2009 | 19130284 |
| quantitative pcr: a quality control assay for estimation of viable virus content in live attenuated goat pox vaccine. | efficacy of live viral vaccine and vaccine-induced sero-conversion depends on the optimum number of live virus particles in a vaccine dose, which is one of the important aspects of quality control. in the present study, taqman probe quantitative polymerase chain reaction (qpcr) based on conserved dna pol gene of capripoxvirus was developed for the quality control of attenuated monovalent goatpox and/or combined attenuated goatpox and peste des petits ruminants (ppr) vaccines. cells infected with ... | 2009 | 20099465 |
| possible control and eradication of peste des petits ruminants from india: technical aspects. | the peste des petits ruminants (ppr) is an acute and highly contagious, notifiable viral disease of sheep and goats that causes substantial morbidity and mortality. there are three cell culture-based live attenuated ppr vaccines available (one from an african isolate and two from indian isolates). the ppr vaccine produced by the indian veterinary research institute has been extensively evaluated in the field and found safe and potent in sheep and goats in india. diagnostic tests, such as the san ... | 2009 | 20391409 |
| a short note on peste des petits ruminants in karnataka, india. | retrospective quantitative analysis of epidemiological data on peste des petits ruminants (ppr) from the department of animal husbandry and veterinary sciences in karnataka, southern india, revealed significant information about the disease in this area. in the nine years between april 1998 and march 2007 a total of 624 outbreaks were reported in the state. with the exception of the 12-month period between april 2001 and march 2002 the disease occurred every year. the study shows clearly that in ... | 2009 | 20462160 |
| status of sheep sera to bluetongue, peste des petits ruminants and sheep pox in a few northern states of india. | bluetongue (bt), peste des petits ruminants (ppr) and sheep pox are the most economically important viral diseases of sheep in india. serum samples obtained from sheep in five northern states of the country were screened for antibody against these agents to explore the extent of spread of these infections. a total of 516 serum samples were screened for the presence of antibodies against bt and ppr viruses. of these, 155 samples were also tested for antibodies against sheep pox virus. bt antibodi ... | 2008 | 20405449 |
| the epidemiology of peste des petits ruminants in pakistan. | peste des petits ruminants (ppr) is an acute and highly contagious viral disease of small ruminants, which is newly emerging in pakistan. information provided by participatory disease surveillance teams was used to develop a database for ppr outbreaks in pakistan. twenty-four villages were selected throughout the country and field investigations were conducted in each village to study the dynamics of the disease. in each area, flocks with a history of ppr were identified and serological sampling ... | 2008 | 19284055 |
| peste des petits ruminants (ppr) in ethiopia: analysis of a national serological survey. | peste des petits ruminants (ppr) is a contagious viral disease of small ruminants in africa and asia. in 1999, probably the largest survey on ppr ever conducted in africa was initiated in ethiopia where 13 651 serum samples from 7 out of the 11 regions were collected and analyzed by competitive enzyme-linked immunosorbent assay (celisa). the objective of this paper is to present the results of this survey and discuss their practical implications for ppr-endemic regions. | 2008 | 18786275 |
| examples of probable host-pathogen co-adaptation/co-evolution in isolated farmed animal populations in the mountainous regions of north vietnam. | in vietnam, for a number of specific geographical and historical reasons, the mountainous areas have preserved an exceptional diversity of wild and domestic animal species of high socioeconomic interest. this endemic genetic diversity fosters a rapid response to environmental change in mostly isolated local communities and, in particular, fosters the constant adaptation of ecosystems common to humans and farmed and wild animal populations and pathogens. during a 2-year study carried out in sever ... | 2008 | 19120225 |
| identification and mapping of a region on the mrna of morbillivirus nucleoprotein susceptible to rna interference. | the morbillivirus genus includes important pathogens such as measles virus (mv), peste des petits ruminants virus (pprv), and rinderpest virus (rpv) and forms a group of antigenically related viruses. the viral nucleoprotein (n) is a well-conserved protein among the genus and plays a central role in the replication of the virus. using a comprehensive approach for sirna screening of the conserved sequences of the n gene, including sequence analysis and functional in vitro tests, we have identifie ... | 2008 | 18634829 |
| persistent detection of peste de petits ruminants antigen in the faeces of recovered goats. | peste des petits ruminants (ppr) disease was confirmed in west african dwarf goats. they were managed symptomatically with antibiotics and antidarrhoeics. following clinical recovery, faeces were collected every week from 40 recovered goats to monitor excretion of the ppr virus haemagglutinins in their faeces. all the 40 recovered goats shed the ppr virus haemagglutinins for 11 weeks post recovery. nine goats (22.5%) continued shedding the viral antigen 12 weeks post recovery. there was correlat ... | 2008 | 18716908 |
| the detection of antibody against peste des petits ruminants virus in sheep, goats, cattle and buffaloes. | monoclonal antibody-based competitive elisa (c-elisa) has been used for the specific measurement of antibodies to peste des petits ruminants (ppr) viruses in sheep, goats, cattle and buffalo. serum samples from sheep (n = 232), goats (n = 428), cattle (n = 43), buffalo (n = 89) were tested. the animals had not been vaccinated against rinderpest or ppr. findings suggested that the sero-positive cases were significantly higher in sheep (51.29%) than in goats (39.02%) (p = 0.002). the overall sero- ... | 2008 | 18716909 |
| rapid detection of peste des petits ruminants (ppr) virus antigen in sudan by agar gel precipitation (agpt) and haemagglutination (ha) tests. | agpt and ha tests were employed for rapid diagnosis of pprv infection in sheep and goats in sudan. forty lymph nodes and spleen samples from suspected cases of ppr in both sheep and goats were examined by agpt and ha tests for detection of pprv antigen. viral antigen was detected from (77.5%) of the samples tested by agpt and (92.5%) tested by ha test. the results of both tests revealed that ha test was more sensitive than agpt for detection of pprv antigen (kappa statistics 0.4366). another adv ... | 2008 | 18509945 |
| effect of sirna mediated suppression of signaling lymphocyte activation molecule on replication of peste des petits ruminants virus in vitro. | signaling lymphocyte activation molecule (slam) expression was inhibited in b95a cell line using sirna and the effect of slam inhibition on peste des petits ruminants virus (pprv) replication and infectivity titre was studied. slam suppression was assessed using real-time pcr and flow cytometry to confirm suppression at the m-rna and protein levels, respectively. three chemically synthesized sirnas were transfected individually using oligofectamine into b95a cell line. this resulted in slam supp ... | 2008 | 18550191 |
| standardization of large scale production of homologous live attenuated ppr vaccine in india. | live attenuated homologous vaccine against peste des petits ruminants of sheep and goats was produced on a large scale basis in roller culture bottles using seed virus developed at the indian veterinary research institute, muktheswar, india. vero cells between 130-150 passages with six percent foetal calf serum were used for the production of vaccine. the cells were infected with 0.01 multiplicity of infection and harvested when the cytopathic effect was 80%. the vaccine was freezedried in order ... | 2008 | 18551773 |
| in situ detection of ppr virus antigen in skin papules around the mouth of sheep experimentally infected with ppr virus. | 2008 | 18557185 | |
| full genome sequences of two virulent strains of peste-des-petits ruminants virus, the côte d'ivoire 1989 and nigeria 1976 strains. | peste-des-petits ruminants virus (pprv) causes acute febrile illness in both farmed and wild small ruminants, with associated mortality rates of 50-80%. pprv is a member of the morbillivirus genus within the paramyxovirus family and although there are many full length genome sequences available for members of this family, their availability for pprv in particular is limited. we have determined the full length sequences representing two virulent strains of pprv, the côte d'ivoire 1989 (ci/89) and ... | 2008 | 18541325 |
| [sequence analysis of the nucleocapsid gene and genome promoter region of peste des petits ruminants virus of chinese origin]. | the n gene and genome promoter nucleotide sequence of a chinese peste des petits rumiants virus (pprv) ("china/tib/gej/07-30") was firstly determined. the length of n gene was 1689 nucleotides with a single open reading frame (orf). the nucleotide and deduced amino acid sequence was compared with the homologous region of other pprv isolates. the nucleotide sequence of the "china/tib/gej/07-30" was 91.7%-97.6% identical to other pprv isolates, while a homology of 94.9%-98.5% could be observed at ... | 2008 | 19226956 |
| seroepidemiology of peste des petits ruminants in sheep and goats of southern peninsular india. | this paper presents the results of a seroepidemiological study carried out between july 2006 and march 2007 to detect the presence of antibodies to peste des petits ruminants (ppr) virus in randomly collected serum samples from sheep and goats in southern peninsular india. the authors used a competitive enzyme-linked immunosorbent assay with a monoclonal antibody developed against a neutralising epitope of the haemagglutinin (ha) protein of the virus. a total of 1,492 sheep sera and 2,068 goat s ... | 2008 | 19284053 |
| identification of functional domains of phosphoproteins of two morbilliviruses using chimeric proteins. | the paramyxovirus p protein is an essential component of the transcriptase and replicase complex along with l protein. in this article, we have examined the functional roles of different domains of p proteins of two closely related morbilliviruses, rinderpest virus (rpv) and peste des petits ruminants virus (pprv). the pprv p protein physically interacts with rpv l as well as rpv n protein when expressed in transfected cells, as shown by co-immunoprecipitation. the heterologous l-p complex is bi ... | 2008 | 18427968 |
| scalable culture systems using different cell lines for the production of peste des petits ruminants vaccine. | peste des petits ruminants (ppr) is considered as one of the major constraints to the productivity of small ruminants in africa and asian countries. currently ppr control is done by vaccination with an attenuated ppr strain (nigeria 75/1) produced in monolayers of vero cells grown in roller bottles or static flasks. this work focuses on the production of a ppr vaccine strain using stirred conditions as an advanced option for process scale-up. non-porous microcarriers (cytodex-1) were used to sup ... | 2008 | 18486286 |
| rapid quality control of a live attenuated peste des petits ruminants (ppr) vaccine by monoclonal antibody based sandwich elisa. | peste des petits ruminants (ppr) is a highly contagious and economically important viral disease of goats and sheep. a homologous vero cell-based attenuated ppr vaccine developed in our laboratory and used extensively throughout the country, is available for control of ppr. the presently used quality control test, titration in vero cells for ppr virus titre in vaccine batches, takes at least 6-8days to determine the quality and dose of vaccine. in this study, 74 freeze-dried ppr vaccine batches ... | 2008 | 17644410 |
| genetic characterization of indian peste des petits ruminants virus (pprv) by sequencing and phylogenetic analysis of fusion protein and nucleoprotein gene segments. | peste des petits ruminants (ppr) is an important viral disease of sheep and goats, endemic in india. the study was undertaken to characterize the local pprv by sequencing fusion (f) protein and nucleoprotein (n) gene segments and phylogenetic analysis, so as to focus on genetic variation in the field viruses. selected regions of pprv genome were amplified from clinical samples collected from 32 sheep and goats by rt-pcr and the resulting amplicons were sequenced for phylogenetic analysis. the ph ... | 2008 | 17850836 |
| potential of antiviral therapy and prophylaxis for controlling rna viral infections of livestock. | with intensification of trade, livestock are increasingly exposed to severe animal diseases caused by a range of rna viruses. recent prime examples include outbreaks of foot-and-mouth disease (fmd), peste des petits ruminants, rift valley fever and bluetongue. to minimise their impact, controlling the spread of virus is of utmost importance. good quality, reliable vaccines exist for some, although not all, of these diseases, but suffer from a set of drawbacks, not the least of which being the ti ... | 2008 | 18035428 |
| detection of peste des petits ruminants virus antigen using immunofiltration and antigen-competition elisa methods. | peste des petits ruminants (ppr) is one of the most economically important diseases affecting sheep and goats in india. an immunofiltration-based test has been developed using either mono-specific serum/monoclonal antibodies (mab) prepared against a recombinant truncated nucleocapsid protein of rinderpest virus (rpv) cross-reactive with ppr virus. this method consists of coating ocular swab eluate from suspected animals onto a nitrocellulose membrane housed in a plastic module, which is allowed ... | 2008 | 18182256 |
| development of one-step real-time rt-pcr assay for detection and quantitation of peste des petits ruminants virus. | in this study, a rapid and specific taqman-based, one-step real-time quantitative reverse transcription pcr (qrt-pcr) has been described for the detection of peste des petits ruminants virus (pprv). primers and probe were designed based on the nucleocapsid protein gene sequence. the real-time qrt-pcr assay was able to detect pprv isolates from very distinct geographical areas (africa, middle east and asia). the specificity of the assay was assessed by including rinderpest virus and other morbill ... | 2008 | 18243345 |
| seroprevalence of, and risk factors for, peste des petits ruminants in sheep and goats in northern jordan. | peste des petits ruminants (ppr) is an economically important disease that affect sheep and goat industry in asia and africa. in this study, we investigated the seroprevalence, and risk factors, of ppr in sheep and goat flocks from five different governorates (irbid, jarash, ajloun, mafraq and zarka) located in northern jordan. serum samples from 929 and 400 sheep and goats, respectively, corresponding to 122 sheep flock and 60 goats flock were collected. seroprevalence was determined using ppr ... | 2008 | 18291541 |
| concurrent peste des petits ruminants virus and pestivirus infection in stillborn twin lambs. | concurrent infection with peste des petits ruminants virus (pprv) and pestivirus was diagnosed in stillborn twin lambs. with the flock history, the findings of epidermal syncytial cells and necrotizing bronchitis/bronchiolitis prompted testing for pprv infection, and pprv antigen was detected by immunohistochemistry (ihc) in the skin, lungs, kidneys, rumen, and thymus. macroscopic anomalies that were typical of border disease included scoliosis, brachygnathism, prognathism, arthrogryposis, hydra ... | 2008 | 18424832 |
| relationship between the level of signaling lymphocyte activation molecule mrna and replication of peste-des-petits-ruminants virus in peripheral blood mononuclear cells of host animals. | basal signaling lymphocyte activation molecule (slam) expression in the peripheral blood mononuclear cells (pbmcs) of cattle, buffalo, sheep, and goats was correlated with peste-des-petits-ruminants virus (pprv) replication assessed by real-time pcr and virus titers. pbmcs from goats had the highest level of slam mrna followed by sheep, cattle, and buffalo. different breeds of goats had different basal levels of slam mrna. in the pbmcs of studied animals, basal slam expression had high correlati ... | 2008 | 19143479 |
| the epidemiology of peste des petits ruminants in pakistan and possible control policies. | peste des petits ruminants made its first clinical appearance in pakistan during an epidemic in punjab province in 1991, but its level of activity in subsequent years was never systematically recorded. in 2003, 2004 and 2005 teams trained in participatory disease surveillance methods visited a large number of villages throughout pakistan to record the prevalence and impact of key livestock diseases. peste des petits ruminants has emerged from their study as a common and economically damaging dis ... | 2008 | 19284054 |
| prevalence and mortality rate of peste des petitis ruminant (ppr): possible association with abortion in goat. | present study was designed to investigate the prevalence and mortality (%) caused by peste des petitis ruminant (ppr) and its possible association with abortion in goat flocks at different areas of pakistan. a total of 140 animals were samples in the population of 650 which was having 185 deaths (mortality rate = 28 %) from three different regions of the country. there were 58 abortions in the 140 pregnant goats of above said population one hundred & ten (110) serum samples from diseased, recove ... | 2008 | 18509938 |
| mapping the peste des petits ruminants virus nucleoprotein: identification of two domains involved in protein self-association. | for mononegavirales, the template for transcription and replication is not the naked rna but the nucleoprotein (n) encapsidated genomic and anti-genomic rna. because of this central role in the replication of these viruses, n has been the subject of numerous structural and functional mapping studies. here, we report on the cloning of the peste des petits ruminants virus (pprv) n gene into the baculovirus vector and the expression of the protein in insect cells. by electron microscopy observation ... | 2008 | 17875339 |
| laboratory diagnosis of transboundary animal diseases in pakistan. | control of animal and poultry diseases is one of the primary objectives of government livestock departments in pakistan. main emphasis is given to the prevention and control of transboundary animal diseases (tads), i.e. rinderpest, foot and mouth disease (fmd), peste des petits ruminants (ppr) and avian influenza (ai) in the country. recently (2007), the office international des epizooties has certified pakistan as a rinderpest-free country but other tads are still a threat to the livestock and ... | 2008 | 18666961 |
| participatory epidemiology in disease surveillance and research. | participatory epidemiology is the application of participatory methods to epidemiological research and disease surveillance. it is a proven technique which overcomes many of the limitations of conventional epidemiological methods, and has been used to solve a number of animal health surveillance and research problems. the approach was developed in small-scale, community animal health programmes, and then applied to major international disease control efforts. the global rinderpest eradication pr ... | 2007 | 18293603 |
| reverse genetics for peste-des-petits-ruminants virus (pprv): promoter and protein specificities. | peste-des-petits-ruminants virus (pprv) (family paramyxoviridae, genus morbillivirus) causes an acute febrile illness in sheep and goats resulting in significant morbidity and mortality in infected herds. the paramyxoviruses all have negative sense, non-segmented rna genomes and their host range and pathogenic determinants have been extensively studied using reverse genetics. this technology also enables a more rational approach to be taken with respect to vaccine design. in order to initiate th ... | 2007 | 17350130 |
| mixed infection of peste des petits ruminants and orf on a goat farm in shahjahanpur, india. | 2007 | 17384295 | |
| the threat of peste des petits ruminants: progress in vaccine development for disease control. | peste des petits ruminants (ppr) is a highly contagious animal disease caused by a virus in the genus morbillivirus, family paramyxoviridae. this infection is responsible for high morbidity and mortality in sheep and goats and in some small wild ruminant species. the huge number of small ruminants, which are reared in the endemic areas makes ppr a serious disease threatening the livelihood of poor farmers. taking advantage of the closely relationship between rinderpest and ppr viruses, the atten ... | 2007 | 17399862 |
| rescue of a chimeric rinderpest virus with the nucleocapsid protein derived from peste-des-petits-ruminants virus: use as a marker vaccine. | the nucleocapsid (n) protein of all morbilliviruses has a highly conserved central region that is thought to interact with and encapsidate the viral rna. the c-terminal third of the n protein is highly variable among morbilliviruses and is thought to be located on the outer surface and to be available to interact with other viral proteins such as the phosphoprotein, the polymerase protein and the matrix protein. using reverse genetics, a chimeric rinderpest virus (rpv)/peste-des-petits-ruminants ... | 2007 | 17554036 |
| natural peste des petits ruminants virus infection: novel pathologic findings resembling other morbillivirus infections. | the present study describes pathologic and virologic findings in 15 sheep and 6 goats that died of natural peste des petits ruminants virus infection in turkey. pathologic findings included erosive-ulcerative stomatitis, fibrino-necrotic tracheitis, bronchointerstitial pneumonia, multifocal coagulation necroses in the liver, and severe lymphocytolysis in lymphoid tissues. syncytial cells were conspicuous, especially in the oral mucosa, pulmonary alveoli, liver, and lymphoid tissues. in addition ... | 2007 | 17606509 |
| control of ruminant morbillivirus replication by small interfering rna. | peste-des-petits-ruminants virus (pprv) and rinderpest virus (rpv) are two morbilliviruses of economic relevance in african and asian countries. although efficient vaccines are available for both diseases, they cannot protect the animals before 14 days post-vaccination. in emergencies, it would be desirable to have efficient therapeutics for virus control. here, two regions are described in the nucleocapsid genes of pprv and rpv that can be targeted efficiently by synthetic short interfering rna ... | 2007 | 17622636 |
| early detection of viral excretion from experimentally infected goats with peste-des-petits ruminants virus. | we observed 15 goats for 9 days after subcutaneous infection with 10(3) tcid(50) with isolates of peste-des-petits ruminants virus from africa and india and five concurrent, uninfected control goats. typical clinical signs of the infection were present in all 15 infected goats by day 8 and in most by day 6 and some signs were present by day 4. however, 6 out of 15 goats already have detectable virus shedding by day 3 and four more were shedding by day 4 and every goat had virus shedding for at l ... | 2007 | 17064800 |
| development of 2 types of competitive enzyme-linked immunosorbent assay for detecting antibodies to the rinderpest virus using a monoclonal antibody for a specific region of the hemagglutinin protein. | a competitive enzyme-linked immunosorbent assay (c-elisa) has been developed and standardized for the detection of antibodies to the rinderpest virus (rpv) in sera from cattle, sheep, and goats. the test is specific for rinderpest because it does not detect antibodies to peste-des-petits-ruminants virus (pprv). the test depends on the ability of the monoclonal antibody (mab) directed against the hemagglutinin (h) protein of rpv to compete with the binding of rpv antibodies in the positive serum ... | 2007 | 17668032 |
| long-term storage at tropical temperature of dried-blood filter papers for detection and genotyping of rna and dna viruses by direct pcr. | in tropical countries the diagnosis of viral infections of humans or animals is often hampered by the lack of suitable clinical material and the necessity to maintain a cold chain for sample preservation up to the laboratory. this study describes the use of filter papers for rapid sample collection, and the molecular detection and genotyping of viruses when stored over long periods at elevated temperatures. infected blood was collected on filter papers, dried and stored at different temperatures ... | 2007 | 17714797 |
| synthesis, dna binding and antiviral activity of new uracil, xanthine, and pteridine derivatives. | some new 6-amino-1,3-dimethyl-5-(substituted methylidene)aminouracils were synthesized. most of them were cyclized with triethyl orthoformate as a one-carbon source to afford 1,3-dime-thyl-6-substituted pteridine derivatives. certain uracils gave xanthine instead of the expected pteridine derivatives upon using another one-carbon source such as triethyl orthoacetate or triethyl orthobenzoate. the nucleic acid binding assay revealed that some new compounds showed high affinity, chelation, and fra ... | 2007 | 17206606 |
| development of an indirect elisa for the detection of antibodies against peste-des-petits-ruminants virus in small ruminants. | peste des petits ruminants (ppr) is an acute, febrile, highly contagious and economically important viral disease of small ruminants. a polyclonal antibody based indirect elisa was developed for detection of antibodies to ppr virus in the serum samples of goats and sheep using purified ppr viral antigen propagated in vero cell culture. a threshold (cut-off) value was set as twice the mean of the negative population based on the distribution of known negative serum samples in respect of ppr virus ... | 2007 | 17216310 |
| peste des petits ruminants (ppr) outbreak in tajikistan. | the occurrence of outbreaks of peste des petits ruminants (ppr) in three districts of tajikistan is described. the causal strain (ppr tajikistan) was characterized and the sequence of its n gene was compared with that of 43 other strains isolated since 1968 in africa, the middle east and asia. the study demonstrated (1) the value of the n gene as a target in comparing isolates obtained over an extended period of evolution, and (2) that clustering was related to the geographical origin of strains ... | 2007 | 17321539 |
| evaluation of the virulence of some strains of peste-des-petits-ruminants virus (pprv) in experimentally infected west african dwarf goats. | different isolates of peste-des-petits-ruminants virus (pprv) from outbreaks in africa and india were investigated for virulence in west african dwarf goats in the ivory coast. six groups of five animals received a virulent suspension of various strains of virus at a concentration of 10(3) tcid(50)/ml and the goats were observed for 15 days after infection. the côte-d'ivoire 89 (ci89), guinea conakry and bissau guinea pprv strains caused a peracute disease; the india-calcutta strain caused acute ... | 2007 | 16310383 |
| importance of the extracellular and cytoplasmic/transmembrane domains of the haemagglutinin protein of rinderpest virus for recovery of viable virus from cdna copies. | a specific interaction between the f and h proteins is required to enable fusion of the virus and host cell membranes and in some cases these proteins are not interchangeable between related viruses of the family paramyxoviridae. for example, the f and h proteins of two ruminant morbilliviruses, rinderpest virus (rpv) and peste-des-petits-ruminants virus (pprv), are not interchangeable since viable virus could not be rescued from cdna constructs where an individual glycoprotein gene of rpv was r ... | 2006 | 16343677 |
| marmoset b95a cells: a sensitive system for cultivation of peste des petits ruminants (ppr) virus. | 2006 | 16362615 | |
| comparison of diagnostic efficacy of a monoclonal antibody-based competitive elisa test with a similar commercial test for the detection of antibodies to peste des petits ruminants (ppr) virus. | 2006 | 16437308 | |
| analysis of the matrix protein gene sequence of the asian lineage of peste-des-petits ruminants vaccine virus. | the m gene nucleotide sequence of an indian peste-des-petits ruminants (pprv) vaccine virus ("pprv sungri/96") belonging to asian lineage was determined. the gene is 1476 nucleotides long with a single open reading frame (orf). the nucleotide and predicted amino acid sequence was compared with the homologous region of the african lineage vaccine virus "pprv/nigeria/75/1". the nucleotide sequence of the "pprv sungri/96" was 86% identical to that of "pprv/nigeria/75/1", while a homology of 93% and ... | 2006 | 16297575 |
| matrix protein and glycoproteins f and h of peste-des-petits-ruminants virus function better as a homologous complex. | the matrix (m) protein of paramyxoviruses forms an inner coat to the viral envelope and serves as a bridge between the surface glycoproteins (f and h) and the ribonucleoprotein core. previously, a marker vaccine (rpv-pprfh) was produced for the control of peste des petits ruminants (ppr) disease, where the f and h genes of rinderpest virus (rpv) were replaced with the equivalent genes from peste-des-petits-ruminants virus (pprv); however, this virus grew poorly in tissue culture. the poor growth ... | 2006 | 16760405 |
| control of peste des petits ruminants and poverty alleviation? | peste des petits ruminants (ppr) is a contagious and often fatal viral disease of sheep and goats and also wild small ruminants. the ppr virus is distinct from but closely related to rinderpest virus and both belong to the morbivillivirus genus within the family paramyxoviridae. ppr is a contagious transboundary disease with a significant impact on rural poor farmers. its control should therefore be considered in programs that aim at alleviating poverty in developing countries. | 2006 | 17123358 |
| foot and mouth disease, rinderpest, peste des petits ruminants and avian influenza in the erbil, dohuk, and sulaimania governorates of iraq. | foot and mouth disease (fmd) outbreaks in the northern part of iraq have been reported. the outbreaks in 1998/1999 were due to type o virus and the disease was associated with significant mortalities especially in young calves and lambs. furthermore, 10 fmd-like incidents in cattle were reported in 2002 in the three northern governorates. however, these cases were not associated with mortalities and the disease remained localized in the primary foci. type a virus was isolated from the tested sam ... | 2006 | 17123362 |
| regional and international approaches on prevention and control of animal transboundary and emerging diseases. | transboundary animal diseases pose a serious risk to the world animal agriculture and food security and jeopardize international trade. the world has been facing devastating economic losses from major outbreaks of transboundary animal diseases (tads) such as foot-and-mouth disease, classical swine fever, rinderpest, peste des petits ruminants (ppr), and rift valley fever. lately the highly pathogenic avian influenza (hpai) due to h5n1 virus, has become an international crisis as all regions arou ... | 2006 | 17135498 |
| interference in the vaccination of cattle against rinderpest virus by antibodies against peste des petits ruminants (ppr) virus. | the ability of the attenuated vaccine 75/1 of peste des petits ruminants to interfere with rinderpest vaccination in cattle was investigated experimentally. young cattle (93) were selected and tested as being negative for antibodies against rp or ppr viruses. these were vaccinated with the peste des petits ruminants attenuated vaccine strain ppr75/1. all animals produced specific antibodies against the peste des petits ruminants vaccine after one or two doses. the cattle were then vaccinated wit ... | 2006 | 16757064 |
| comparative nucleotide sequence analysis of the phosphoprotein gene of peste des petits ruminants vaccine virus of indian origin. | the nucleotide sequences of the phosphoprotein (p) gene of peste des petits ruminants (pprv) vaccine virus (pprv sungri/96) belongs to asian lineage have been determined and the deduced amino acid sequences were compared with another vaccine strain pprv/nigeria75/1 and with those of the other morbilliviruses. the 1652 nucleotides of the p gene encode a phosphoprotein of 509 amino acid residues (from nucleotide numbers 60 to 1587), which is 91% identical to that of pprv/nigeria75/1. the c protein ... | 2006 | 16289265 |
| immunomodulatory properties of morbillivirus nucleoproteins. | morbillivirus infections have been known for a long time to be associated with an acute immunosuppression in their natural hosts. here, we show that recombinant morbillivirus nucleoproteins from canine distemper virus, peste-des-petits-ruminants virus, and rinderpest virus bind b-lymphocytes from dogs, goats, and cattle, respectively, similarly to measles virus nucleoprotein in humans. the use of surface plasmon resonance imaging allowed the real time detection of differential interactions betwe ... | 2006 | 16817775 |
| a bivalent vaccine against goat pox and peste des petits ruminants induces protective immune response in goats. | safety and immunogenicity of an experimental combined vaccine comprising attenuated strains of peste des petits ruminants virus (pprv) and goat poxvirus (gtpv) was evaluated in goats. goats immunized subcutaneously with 1 ml of vaccine consisting of 10(3) tcid(50) of each of pprv and gtpv were monitored for clinical and serological responses for a period of 4 weeks postimmunization (pi) and postchallenge (pc). specific antibodies directed to both gtpv and pprv could be demonstrated by indirect e ... | 2006 | 16828938 |
| one-step multiplex rt-pcr assay for the detection of peste des petits ruminants virus in clinical samples. | a single-tube one-step multiplex rt-pcr was standardized to amplify both 337 bp and 191 bp fragments of n and m genes of peste des petits ruminants virus (pprv), respectively, and only a 337 bp fragment of n gene of rinderpest virus (rpv). the rt-pcr using purified viral rna was easily adopted for direct detection of pprv in clinical field samples and its differentiation from rpv. the amplified n and m gene products were confirmed to be pprv- and rpv-specific by their size in 1.5% agarose gel an ... | 2006 | 16838207 |
| identification of t-helper and linear b epitope in the hypervariable region of nucleocapsid protein of pprv and its use in the development of specific antibodies to detect viral antigen. | peste des petits ruminants is a highly contagious viral disease of small ruminants making its diagnosis difficult from the similar symptoms of rinderpest. computer based prediction algorithms was applied to identify antigenic determinants on the nucleocapsid (n) protein of pprv. specificity and antigenicity of each peptide was evaluated by solid phase elisa. six specific peptide sequences were evaluated in multiple antigenic peptide (map) form and immune response was evaluated by supplementing u ... | 2006 | 16962260 |
| development and characterization of a stable vero cell line constitutively expressing peste des petits ruminants virus (pprv) hemagglutinin protein and its potential use as antigen in enzyme-linked immunosorbent assay for serosurveillance of pprv. | we developed and characterized a stable vero cell line constitutively expressing peste des petits ruminants virus (pprv) hemagglutinin (h) protein and assessed its potential use as diagnostic antigen in enzyme-linked immunosorbent assay (elisa). pprv h gene of the vaccine strain (sungri-96) was amplified by reverse transcription (rt)-pcr, cloned into a eukaryotic expression vector (ptarget), and subsequently transfected and expressed in vero cells. a stable vero cell line was developed after 20 ... | 2006 | 17050742 |
| sequence analysis of the nucleoprotein gene of asian lineage peste des petits ruminants vaccine virus. | the complete nucleotide sequence of the nucleocapsid (n) protein of the peste-des-petits ruminants vaccine virus (pprv sungri/96) belonging to the asian lineage was determined. the gene was 1692 nucleotides in length and encoded a polypeptide of 525 amino acids. the pprv sungri/96 n gene has a nucleotide homology of 92% for pprv nigeria 75/1 to 55.5% for canine distemper virus. at amino acid level the homology was 94.1% with pprv nigeria 75/1, while with other morbilliviruses, pprv sungri/96 had ... | 2006 | 17139548 |
| the m and n genes-based simplex and multiplex pcrs are better than the f or h gene-based simplex pcr for peste-des-petits-ruminants virus. | nucleocapsid (n), matrix (m) and hemagglutinin (h) genes-based simplex pcrs and an n and m genes-based multiplex pcr were developed for detection of peste-des-petits-ruminants virus (pprv). the m gene pcr was the most sensitive, followed by n, h and an already described fusion (f) gene pcrs, as they could detect the virus in samples with titers of 101, 102, 104and 105 tcid50/ml, respectively. the multiplex pcr was as sensitive as the m gene pcr, but it had the advantage of differentiating pprv f ... | 2006 | 17177605 |