Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| sylvatic foci of the chagas disease vector triatoma infestans in chile: description of a new focus and challenges for control programs. | triatoma infestans is one of the main domestic vectors of chagas disease. reports of wild habitat occurrences have recently increased. in chile, after a successful elimination campaign of t. infestans domestic infestation, a sylvatic focus was reported in bromeliads in the metropolitan region. here, we report a new focus of sylvatic t. infestans inhabiting rock piles in the valparaíso region in central chile. all t. infestans captured were nymphal instars living among the stones, which were inha ... | 2010 | 20835609 |
| statistical phylogeography of chagas disease vector triatoma infestans: testing biogeographic hypotheses of dispersal. | chagas disease is one of the most important vector-borne diseases in latin america. the disease, caused by the flagellate protozoan trypanosoma cruzi, is commonly transmitted to humans by triatoma infestans in south america. using mitochondrial dna sequences, we assessed alternative biogeographic scenarios of dispersal of t. infestans using coalescence simulations. we also assessed phylogeographic structure and spatial genetics of t. infestans in chile. two major routes of dispersal in southern ... | 2010 | 20920609 |
| phenotypic variability and population structure of peridomestic triatoma infestans in rural areas of the arid chaco (western argentina): spatial influence of macro- and microhabitats. | abstract triatoma infestans, one of the main vectors of trypanosoma cruzi, is strongly associated with rural human ecotopes. infested peridomestic places could act as a source of house infestation as a result of the movement of vectors among habitats. the quantitative study of phenotypic traits and nutritional variables could be informative about the population structure and the relative mobility of vectors. this is the first approach toward analyzing the phenetic characteristics of t. infestans ... | 2010 | 20925525 |
| residual effect of a micro-encapsulated formulation of organophosphates and piriproxifen on the mortality of deltamethrin resistant triatoma infestans populations in rural houses of the bolivian chaco region. | the bolivian chaco is part of the endemic region of chagas disease and an area where pyrethroid resistant triatoma infestans (hemiptera: reduviidae) populations has been reported. the world health organization identified these resistant populations as an important focus for research. the objective of this study was to evaluate the residual effect of a micro-encapsulated formulation containing organophosphate active ingredients and a juvenile hormone analogue (inesfly 5a igr) on the mortality of ... | 2010 | 20944988 |
| vectorial capacity of triatoma guasayana (wygodzinsky & abalos) (hemiptera: reduviidae) compared with two other species of epidemic importance. | triatoma guasayana (wygodzinsky & abalos) is a peridomestic triatomine with epidemiological importance in bolivia, that may play an important role in the transmission of trypanosoma cruzi (chagas). in this study, two parameters of vectorial capacity were evaluated: the interval of feeding-defecation time and metacyclogenesis, in adult males and females and nymphal instars ii to v of t. guasayana with comparisons with triatoma infestans (klug) and t. sordida (stal). the results showed a close rel ... | 2010 | 21120391 |
| new findings of insecticide resistance in triatoma infestans (heteroptera: reduviidae) from the gran chaco. | chagas disease is a major health concern in latin america, and triatoma infestans (klug, 1834) is responsible for the majority of cases of chagas disease in the continent. after the discovery of deltamethrin highly resistant populations in the neighboring areas to yacuiba (bolivia) and salvador mazza (argentina), we studied t. infestans populations north and southward, with the aim of describing the range of the resistant area. in addition, tests were conducted to describe the susceptibility to ... | 2010 | 21175056 |
| the effects of cypermethrin pour-on and piperonyl butoxide on triatoma infestans under laboratory conditions. | the effect of exposing triatoma infestans to chickens treated with cypermethrin pour-on combined with piperonyl butoxide (pbo) was studied. four groups of treated chickens and one control group were used. each treatment received 1 or 2 ml of the cypermethrin formulation with and without pbo. independent groups of nymphs were fed 1, 7, 15, 30, and 45 d after the treatment application. blood intake was estimated after each feeding occasion. up to 15 d after the pour-on application, high mortality ... | 2010 | 21175064 |
| entomological factors affecting the low endemicity of chagas disease in nazca, southwestern peru. | chagas disease is prevalent in peru. the province of nazca, in the southwestern region of the country, shows a high intradomiciliary infestation rate of triatoma infestans bugs. although the vector is present, the number of chagas disease cases appears to be much lower than those reported in the neighboring region of arequipa. we examined 624 t. infestans from nazca to determine the current trypanosoma cruzi infection rates, and found that no bugs were infected with this parasite. these results ... | 2010 | 19874184 |
| vertical transmission of trypanosoma cruzi in the province of choapa, iv region, chile: preliminary report (2005-2008). | congenital chagas disease acquired special importance in chile after the certification of the control of triatoma infestans and transmission by blood donors affected with trypanosoma cruzi. in order to establish adequate protocols for intervention and control in infected mother-neonate pairs in endemic zones of chagas disease, we present partial results (2005-2008) of a pilot project which is being carried out in the province of choapa, iv region, chile, whose objectives are: determine the curre ... | 2010 | 21249297 |
| infection and invasion mechanisms of trypanosoma cruzi in the congenital transmission of chagas' disease: a proposal. | chagas' disease is produced by the haemophlagelated protozoan trypanosoma cruzi and transmitted by haematophages insects such as triatoma infestans (vinchuca). due to vector control, congenital transmission gains importance and is responsible for the presence and expansion of this disease in non-endemic areas. the mechanisms of congenital infection are uncertain. it has been suggested that the parasite reaches the fetus through the bloodstream by crossing the placental barrier, and that congenit ... | 2010 | 21249302 |
| allatotropin-like peptide in malpighian tubules: insect renal tubules as an autonomous endocrine organ. | malpighian tubules (mts) are recognised as the main excretory organ in insects, ensuring water and mineral balance. haematophagous insects incorporate with each meal a large quantity of blood, producing a particularly large volume of urine in a few hours. in the present study, we report the presence of an allatotropin-like (at-like) peptide in mts of triatoma infestans (klug). the at-like content in mts decreased during the first hours after blood-intake, correlating with the post-prandial diure ... | 2009 | 19118556 |
| mitochondrial 16s dna variation in populations of triatoma infestans from argentina. | variation in the mtdna 16s ribosomal rna gene in populations of triatoma infestans (klug) was surveyed. dna sequence comparisons yielded 18 haplotypes among 130 individuals from 16 localities that represent a large proportion of the range of t. infestans in argentina. the most common genotype in all populations was found in 76.9% of individuals and two other haplotypes were shared among different populations. the remaining 15 haplotypes were present exclusively in one of the populations, suggest ... | 2009 | 19067796 |
| changes in the corpora allata and epidermal proliferation along the fourth instar of the chagas disease vector triatoma infestans. | triatoma infestans, a blood-feeding insect, synchronises physiological mechanisms leading to moult with food intake. since the corpora allata are important in moult and metamorphosis regulation, we have studied morphological changes in 4th instar nymphs (gland size, cell density, percent of animals showing mitoses and cell size). changes were correlated with the effect of precocene ii, epidermal proliferation, and with the extent of the "head critical period". based on morphological grounds, thr ... | 2009 | 20067030 |
| behavioral and electrophysiological responses of triatoma brasiliensis males to volatiles produced in the metasternal glands of females. | in many insects, mate finding is mediated by volatile sex pheromones, but evidence for this phenomenon in triatomines (heteroptera: reduviidae) is still fragmentary. recently, it was shown that metasternal glands (mgs) are involved in producing signals related to the sexual communication of triatoma infestans and rhodnius prolixus. based on this, we tested whether mg volatiles could be involved in the sexual communication of triatoma brasiliensis. odor-mediated orientation responses were studied ... | 2009 | 19902303 |
| effects of topical application of fipronil spot-on on dogs against the chagas disease vector triatoma infestans. | we assessed the insecticidal effects of fipronil spot-on applied to experimental dogs on the blood-feeding success and other vital parameters of the trypanosoma cruzi vector triatoma infestans. in the first trial, the cumulative mortality of 30 third or fourth instar nymphs exposed to eight fipronil-treated dogs differed significantly from those exposed to untreated dogs at 1 week post-treatment, but not at baseline or at 2-6 weeks post-treatment. in the second trial, the effects of multiple exp ... | 2009 | 19004462 |
| differential distribution of trypanosoma cruzi clones in human chronic chagasic cardiopathic and non-cardiopathic individuals. | pcr and southern blot hybridization were used to determine the distribution of trypanosoma cruzi clones in 37 chronic chagasic cardiopathic and non-cardiopathic patients. parasite dna amplified from peripheral blood or dejections of triatoma infestans fed on patient blood was hybridized with probes containing hypervariable minicircle nucleotide sequences capable of detecting three sublineages of t. cruzi. probes z-i and z-iib detect unique sequences in lineages tci and tciib, respectively. probe ... | 2009 | 19059372 |
| trypanosoma cruzi: multiplex pcr to detect and classify strains according to groups i and ii. | a multiplex pcr was developed for simultaneous detection of trypanosoma cruzi dna and classification of the parasite strain into groups i and ii. as little as 10fg of t. cruzi dna could be detected by multiplex pcr. the technique was shown to be specific for t. cruzi dna, since no pcr amplification products were obtained with dna from other tripanosomatid species. multiplex pcr was validated by assaying genomic dna from 34 strains of t. cruzi that had been previously characterized; 24 blood samp ... | 2009 | 19133262 |
| cost-effectiveness of chagas disease vector control strategies in northwestern argentina. | control and prevention of chagas disease rely mostly on residual spraying of insecticides. in argentina, vector control shifted from a vertical to a fully horizontal strategy based on community participation between 1992 and 2004. the effects of such strategy on triatoma infestans, the main domestic vector, and on disease transmission have not been assessed. | 2009 | 19156190 |
| epicuticular lipids induce aggregation in chagas disease vectors. | abstract: | 2009 | 19173716 |
| prevalence and distribution of parasites and pathogens of triatominae from argentina, with emphasis on triatoma infestans and triatoma virus trv. | chagas' disease is the most important endemic arthropod-zoonosis in argentina with an estimated 1.6 million people infected with the causative agent trypanosoma cruzi. triatoma infestans is the main vector of chagas' disease in argentina. a survey for parasites and pathogens of triatominae was conducted from august 2002 to february 2005. collections of insects were made in domiciles, peridomiciles, and in the natural habitats of the triatominae. insects from these collections were dissected and ... | 2009 | 19660466 |
| susceptibility of triatoma infestans to deltamethrin in rio grande do sul, brazil. | strategies for controlling chagas disease are based on spraying infested houses with pyrethroid insecticides. however, the intense use of these insecticides has promoted resistance of triatoma infestans and, in argentina, bolivia and southern brazil, low levels of resistance have been reported. due to the persistence of t. infestans in the state of rio grande do sul (rs), we evaluated the occurrence of deltamethrin resistance in four strains from different municipalities in comparison to two sus ... | 2009 | 19722097 |
| biology, diversity and strategies for the monitoring and control of triatomines--chagas disease vectors. | despite the relevant achievements in the control of the main chagas disease vectors triatoma infestans and rhodnius prolixus, several factors still promote the risk of infection. the disease is a real threat to the poor rural regions of several countries in latin america. the current situation in brazil requires renewed attention due to its high diversity of triatomine species and to the rapid and drastic environmental changes that are occurring. using the biology, behaviour and diversity of tri ... | 2009 | 19753457 |
| sustainability of vector control strategies in the gran chaco region: current challenges and possible approaches. | sustainability has become a focal point of the international agenda. at the heart of its range of distribution in the gran chaco region, the elimination of triatoma infestans has failed, even in areas subject to intensive professional vector control efforts. chagas disease control programs traditionally have been composed of two divorced entities: a vector control program in charge of routine field operations (bug detection and insecticide spraying) and a disease control program in charge of scr ... | 2009 | 19753458 |
| wild triatoma infestans, a potential threat that needs to be monitored. | the current persistence of triatoma infestans, and therefore of chagas disease transmission, in the andean valleys of bolivia and the gran chaco (precisely where wild populations of the vector are widespread), indicates a possible relationship between these two occurrences. this paper provides an overview of the current knowledge regarding wild t. infestans in bolivia. the different morphs of the wild vector, their known distributions and some traits of their biology and ecology are presented. p ... | 2009 | 19753459 |
| sylvatic triatominae: a new challenge in vector control transmission. | over the last 10 years, uruguay, chile and brazil have been certified as being free from disease transmission by triatoma infestans, the main domiciliated vector for chagas disease in the southern cone countries. this demonstrates that programmes addressing the vector for the disease's transmission are effective. these programmes have resulted in a dramatic decrease in the incidence of chagas disease in latin america. guatemala was certified a few months ago as being free from disease transmissi ... | 2009 | 19753461 |
| temporal variations of wing size and shape of triatoma infestans (hemiptera: reduviidae) populations from northwestern argentina using geometric morphometry. | wing geometric morphometry of triatoma infestans (klug) (hemiptera: reduviidae) populations in northwestern argentina showed that individual collection sites represent the discrete unit where metric differentiation took place. here we studied temporal variations in wing size and shape of t. infestans populations from defined capture sites on three occasions between 2000 and 2003. bugs collected from domiciles and/or storerooms had significantly larger right-wing centroid size than bugs collected ... | 2009 | 19769028 |
| temporal dynamics of flight muscle development in triatoma infestans (hemiptera: reduviidae). | triatoma infestans klug is dimorphic for flight muscles. this dimorphism may affect flight dispersal, reinfestation patterns, and transmission risk. to understand the contributions of genes and environment to morph determination, it is first necessary to characterize the temporal dynamics of flight muscle development. field-collected t. infestans adults were dissected 20 or 100 d after collection, and those collected as nymphs were dissected at approximately 4, 15, or 44 d after the imaginal mol ... | 2009 | 19769031 |
| first finding of melanic sylvatic triatoma infestans (hemiptera: reduviidae) colonies in the argentine chaco. | triatoma infestans (klug), the most important vector of chagas disease in southern south america, is a highly domiciliated species with well-known sylvatic foci only in the bolivian andean valleys and in the bolivian chaco, where melanic insects designated as "dark morphs" were found. after the tentative identification of two melanic bugs collected from parrot nests in a forest reserve in the argentine chaco as t. infestans, we conducted an intensive search there using mouse-baited sticky traps ... | 2009 | 19769054 |
| immunogenic salivary proteins of triatoma infestans: development of a recombinant antigen for the detection of low-level infestation of triatomines. | triatomines are vectors of trypanosoma cruzi, the etiological agent of chagas disease in latin america. the most effective vector, triatoma infestans, has been controlled successfully in much of latin america using insecticide spraying. though rarely undertaken, surveillance programs are necessary in order to identify new infestations and estimate the intensity of triatomine bug infestations in domestic and peridomestic habitats. since hosts exposed to triatomines develop immune responses to sal ... | 2009 | 19841746 |
| antibody responses of domestic animals to salivary antigens of triatomainfestans as biomarkers for low-level infestation of triatomines. | hematophagous arthropods such as triatoma infestans, the vector of trypanosoma cruzi, elicit host-immune responses during feeding. characterization of antibody responses to salivary antigens offers the potential to develop immunologically based monitoring techniques for exposure to re-emergent triatomine bug populations in peridomestic animals. igg-antibody responses to the salivary antigens of t.infestans have been detected in chickens as soon as 2 days after the first exposure to five adult bu ... | 2009 | 19248784 |
| spatial stratification of house infestation by triatoma infestans in la rioja, argentina. | vectorial transmission of chagas disease has been decreasing over the past few decades because of effective vector control programs in the southern cone of south america. however, the disease is still actively transmitted within the gran chaco region. in this area, vector populations are abundant and highly prevalent in poor rural houses. this study analyses the spatial pattern of rural house infestation by triatoma infestans in a 56,000 km(2) area in the province of la rioja, argentina, before ... | 2009 | 19270290 |
| predominance of trypanosoma cruzi genotypes in two reservoirs infected by sylvatic triatoma infestans of an endemic area of chile. | we report results of trypanosoma cruzi infection and parasite genotypes in the wild octodon degus and synantropic reservoir rattus rattus from an endemic area with sylvatic triatoma infestans as the only detected vector. the infection status was determined by hemi-nested pcr directed to minicircles dna and genotyping by hybridization tests with a panel of five specific probes, including two probes for tci subgroups (clones 19 and 20). o. degus was found infected with 13.3% and mainly with sublin ... | 2009 | 19426670 |
| control of pyrethroid-resistant chagas disease vectors with entomopathogenic fungi. | triatoma infestans-mediated transmission of tripanosoma cruzi, the causative agent of chagas disease, remains as a major health issue in southern south america. key factors of t. infestans prevalence in specific areas of the geographic gran chaco region-which extends through northern argentina, bolivia, and paraguay-are both recurrent reinfestations after insecticide spraying and emerging pyrethroid-resistance over the past ten years. among alternative control tools, the pathogenicity of entomop ... | 2009 | 19434231 |
| strong host-feeding preferences of the vector triatoma infestans modified by vector density: implications for the epidemiology of chagas disease. | understanding the factors that affect the host-feeding preferences of triatomine bugs is crucial for estimating transmission risks and predicting the effects of control tactics targeting domestic animals. we tested whether triatoma infestans bugs prefer to feed on dogs vs. chickens and on dogs vs. cats and whether vector density modified host choices and other vital rates under natural conditions. | 2009 | 19478849 |
| differential effects of alpha-helical and beta-hairpin antimicrobial peptides against acanthamoeba castellanii. | in this work we evaluated the ability of different types of antimicrobial peptides to promote permeabilization and growth inhibition of acanthamoeba castellanii trophozoites, which cause eye keratitis. we used cationic alpha-helical peptides p5 and p6, corresponding to the n-terminus of the pore-forming protein from triatoma infestans, a blood-sucking insect, and a beta-hairpin amphipathic molecule (gomesin), of the spider acanthoscurria gomesiana haemocytes. a. castellanii permeabilization was ... | 2009 | 19490729 |
| response of triatoma infestans to pour-on cypermethrin applied to chickens under laboratory conditions. | this article reports the effects of a pour-on formulation of cypermethrin (6% active ingredient) applied to chickens exposed to triatoma infestans, the main vector of chagas disease in rural houses of the gran chaco region of south america. this study was designed as a completely random experiment with three experimental groups and five replicates. third instar nymphs were fed on chickens treated with 0, 1 and 2 cc of the formulation. nymphs were allowed to feed on the chickens at different time ... | 2009 | 19547876 |
| experimental evaluation of insecticidal paints against triatoma infestans (hemiptera: reduviidae), under natural climatic conditions. | abstract: | 2009 | 19586532 |
| spatial re-establishment dynamics of local populations of vectors of chagas disease. | prevention of chagas disease depends mainly on control of the insect vectors that transmit infection. unfortunately, the vectors have been resurgent in some areas. it is important to understand the dynamics of reinfestation where it occurs. here we show how continuous- and discrete-time models fitted to patch-level infestation states can elucidate different aspects of re-establishment. triatoma infestans, the main vector of chagas disease, reinfested sites in three villages in northwest argentin ... | 2009 | 19636363 |
| molecular population genetics and phylogeography of the chagas disease vector triatoma infestans in south america. | knowledge of the genetic variability, population structure, and evolutionary history of triatoma infestans may be useful for developing rational vector control strategies. a 661-bp fragment of the mitochondrial gene cytochrome oxidase i (coi) was sequenced and analyzed in bugs from argentina, uruguay, peru, and bolivia, including peridomestic, domestic, andean, and chaco sylvatic bugs. a total of 48 polymorphic sites among 37 haplotypes were described. nucleotide variation fluctuated among sampl ... | 2009 | 19645282 |
| differential tissue and flight developmental expression of glycerol-3-phosphate dehydrogenase isozymes in the chagas disease vector triatoma infestans. | glycerol-3-phosphate dehydrogenase (gpdh) isozymes are differentially expressed among tissues and during flight development. gpdh-1 is involved in the flight-muscle metabolism and gpdh-2 provides precursors for lipid biosynthesis in many tissues. we have isolated and characterized from triatoma infestans, a chagas disease vector, two cdnas encoding for gpdh-1 and gpdh-2 isozymes. the inferred amino acid sequences showed high identity with other gpdh sequences from flying insects. a gpdh-2 transc ... | 2008 | 18606760 |
| phenotypic variability associated to genomic changes in the main vector of chagas disease in the southern cone of south america. | this study shows for the first time, the relationship between chromosomic and meristic quantitative characters of triatoma infestans, the main vector of chagas disease in the southern cone of south america. the antennal phenotype of 225 adults from five countries and three cytotypes, were analyzed using univariate and multivariate techniques. the study found a clear association between cytotypes and antennal phenotypes. the complexity of the antennal sensilla pattern was higher in the non-andean ... | 2008 | 18328454 |
| study of the nucleolar cycle and ribosomal rna distribution during meiosis in triatomines (triatominae, heteroptera). | aspects of nucleolar activity during spermatogenesis were assessed in three triatomine species (panstrongylus megistus, rhodnius pallescens and triatoma infestans) using cytochemical and fluorescent staining techniques. toluidine blue and a variant of critical electrolytic concentration (cec) allowed the discrimination of rrna providing structural details of the nucleolus and rna distribution during meiotic cell division. acridine orange fluorochrome staining permitted the differentiation of nuc ... | 2008 | 17976997 |
| nuclear phenotype changes after heat shock in panstrongylus megistus (burmeister). | the nuclear phenotypes of malpighian tubule epithelial cells of male nymphs of the blood-sucking insect, panstrongylus megistus, subjected to short- and long-duration heat shocks at 40oc were analyzed immediately after the shock and 10 and 30 days later. normal nuclei with a usual heterochromatic body as well as phenotypes indicative of survival (unravelled heterochromatin, giants) and death (apoptosis, necrosis) responses were observed in control and treated specimens. however, all nuclear phen ... | 2008 | 10733752 |
| survival and molting incidence after heat and cold shocks in panstrongylus megistus burmeister. | survival and molting incidence were studied after heat (40 degrees c) and cold (0 degree c) shocks in specimens of panstrongylus megistus with the aim of establishing its response to temperature stress under laboratory rearing conditions and to understand occasional changes in the biological characteristics of specimens captured in nature. the response to the thermal shocks was found to vary as a function of the temperature and duration of the shock, developmental phase and sex of the specimens, ... | 2008 | 10029925 |
| localization of cholecystokinin-like immunoreactivity in the central nervous system of triatoma infestans (insecta: heteroptera). | the distribution of cholecystokinin-like immunoreactivity was studied in the central nervous system of the heteropteran insect triatoma infestans using high-sensitivity immunocytochemistry. in the protocerebrum, cck-ir somata were observed in the anteromedial, anterolateral and posterior cell-body layers. the neuropils displayed different densities of immunoreactive neurites. few immunoreactive somata were found in the optic lobe in both the medial and lateral soma rinds, as well as in the proxi ... | 2008 | 18028972 |
| sequence characterization of an unusual lysozyme gene expressed in the intestinal tract of the reduviid bug triatoma infestans (insecta). | antibacterial proteins like lysozyme are important components of the insect non-specific immune response against bacteria. the complementary deoxyribonucleic acid (cdna) encoding a new lysozyme from triatoma infestans, named lysozyme2, has been amplified by polymerase chain reaction and the rapid amplification of cdna ends technique. the gene is expressed in the small intestine of the insect. the deduced protein sequence shows up to 70% similarity to lysozymes from other species. furthermore, th ... | 2008 | 17899195 |
| [on the discovery of triatoma infestans (klug) in pernambuco]. | 2008 | 4881101 | |
| investigations concerning the symbiosis of bacteria in triatoma infestans (klug). | 2008 | 18104272 | |
| a short proregion of trialysin, a pore-forming protein of triatoma infestans salivary glands, controls activity by folding the n-terminal lytic motif. | triatoma infestans (hemiptera: reduviidae) is a hematophagous insect that transmits the protozoan parasite trypanosoma cruzi, the etiological agent of chagas' disease. its saliva contains trialysin, a protein that forms pores in membranes. peptides based on the n-terminus of trialysin lyse cells and fold into alpha-helical amphipathic segments resembling antimicrobial peptides. using a specific antiserum against trialysin, we show here that trialysin is synthesized as a precursor that is less ac ... | 2008 | 18221493 |
| phasing of the triatoma virus diffraction data using a cryo-electron microscopy reconstruction. | the blood-sucking reduviid bug triatoma infestans, one of the most important vector of american human trypanosomiasis (chagas disease) is infected by the triatoma virus (trv). trv has been classified as a member of the cripavirus genus (type cricket paralysis virus) in the dicistroviridae family. this work presents the three-dimensional cryo-electron microscopy (cryo-em) reconstruction of the trv capsid at about 25 a resolution and its use as a template for phasing the available crystallographic ... | 2008 | 18308357 |
| genetic transformation of a corynebacterial symbiont from the chagas disease vector triatoma infestans. | insect-borne diseases have experienced a troubling resurgence in recent years. emergence of resistance to pesticides greatly hampers control efforts. paratransgenesis, or the genetic transformation of bacterial symbionts of disease vectors, is an alternative to traditional approaches. previously, we developed paratransgenic lines of rhodnius prolixus, a vector of chagas disease in central america. here, we report identification of a corynebacterial species as a symbiont of triatoma infestans, a ... | 2008 | 18331732 |
| microsatellites reveal a high population structure in triatoma infestans from chuquisaca, bolivia. | for chagas disease, the most serious infectious disease in the americas, effective disease control depends on elimination of vectors through spraying with insecticides. molecular genetic research can help vector control programs by identifying and characterizing vector populations and then developing effective intervention strategies. | 2008 | 18365033 |
| [about an insecticidal paint for controlling triatoma infestans, in bolivia]. | preliminary evaluations of an insecticidal paint based on diazinon, chlorpyrifos and pyriproxyfen in a micro-encapsulated formulation (inesfly 5a igr) have shown that it has effective and persistent activity against triatoma infestans inside homes and in areas surrounding homes, in a highly infested region of the bolivian chaco. furthermore, the evaluations have highlighted that the product presents good handling characteristics and gives a good appearance to houses and outhouses that have been ... | 2008 | 18368277 |
| molecular genetics reveal that silvatic rhodnius prolixus do colonise rural houses. | rhodnius prolixus is the main vector of chagas disease in venezuela. here, domestic infestations of poor quality rural housing have persisted despite four decades of vector control. this is in contrast to the southern cone region of south america, where the main vector, triatoma infestans, has been eliminated over large areas. the repeated colonisation of houses by silvatic populations of r. prolixus potentially explains the control difficulties. however, controversy surrounds the existence of s ... | 2008 | 18382605 |
| relative contribution of monooxygenase and esterase to pyrethroid resistance in triatoma infestans (hemiptera: reduviidae) from argentina and bolivia. | recently, high resistance to pyrethroid insecticides has been associated with ineffective field treatments against triatoma infestans (klug) (hemiptera: reduviidae) in northern argentina. samples were collected from two areas in argentina (salta and la rioja) and one are in bolivia (yacuiba), and they were subjected to toxicological and biochemical assays. all populations were resistant to deltamethrin, but they showed different profiles to nonpyrethroid insecticides. the salta population showed ... | 2008 | 18402146 |
| differential patterns of insecticide resistance in eggs and first instars of triatoma infestans (hemiptera: reduviidae) from argentina and bolivia. | previous work at our laboratory has indicated high resistance levels to deltamethrin correlated with failures of chemical control in field populations of triatoma infestans (klug, 1834) (hemiptera: reduviidae) in northern argentina and southern bolivia. the aim of the present work was to study the resistance patterns in eggs and first instars of t. infestans in populations from argentina and bolivia. at the egg stage, a population from salvador mazza, argentina, showed the highest resistance rat ... | 2008 | 18533435 |
| molecular epidemiology of domestic and sylvatic trypanosoma cruzi infection in rural northwestern argentina. | genetic diversity of trypanosoma cruzi populations and parasite transmission dynamics have been well documented throughout the americas, but few studies have been conducted in the gran chaco ecoregion, one of the most highly endemic areas for chagas disease, caused by t. cruzi. in this study, we assessed the distribution of t. cruzi lineages (identified by pcr strategies) in triatoma infestans, domestic dogs, cats, humans and sylvatic mammals from two neighbouring rural areas with different hist ... | 2008 | 18585717 |
| phenotypic diversity of triatoma infestans at the microgeographic level in the gran chaco of argentina and the andean valleys of bolivia. | triatoma infestans (klug) is the main vector of trypanosoma cruzi, etiologic agent of chagas disease. the phenotype of the species varies at different geographic scales. the objective of this study was to compare the antennal phenotype of spatially close t. infestans populations and to evaluate its usefulness as a marker of exchange of individuals between populations. the antennal phenotype of 190 t. infestans from argentina and bolivia was analyzed using uni- and multivariate techniques. this s ... | 2008 | 18714865 |
| cytochemical characterization of triatoma infestans and panstrongylus megistus salivary gland cells (hemiptera, reduviidae, triatominae). | the triatominae subfamily has medical sanitary importance, since these insects are vectors of trypanosoma cruzi, etiologic agent of chagas disease, and trypanosoma rangeli, which develops in the salivary glands and it is frequently found in mixed infections with t. cruzi. triatomines of triatoma and panstrongylus genera possess a salivary gland complex composed of a pair with three well differentiated units: the anterior (d1), median (d2) and posterior (d3). saliva is secreted during blood meal ... | 2008 | 18722780 |
| genetic structure of triatoma infestans populations in rural communities of santiago del estero, northern argentina. | to gain an understanding of the genetic structure and dispersal dynamics of triatoma infestans populations, we analyzed the multilocus genotype of 10 microsatellite loci for 352 t. infestans collected in 21 houses of 11 rural communities in october 2002. genetic structure was analyzed at the community and house compound levels. analysis revealed that vector control actions affected the genetic structure of t. infestans populations. bug populations from communities under sustained vector control ... | 2008 | 18773972 |
| rural triatoma rubrovaria from southern brazil harbors trypanosoma cruzi of lineage iic. | triatoma infestans, the main vector of chagas disease, has nearly been eliminated from brazil. nevertheless, other triatominae species are involved in the domiciliation process, including triatoma rubrovaria in rio grande do sul state (rs). previous studies showed that 1.6% of the t. rubrovaria specimens collected at the rural district of quaraí, rs, were naturally infected by trypanosoma cruzi. in this study, five t. cruzi isolates obtained from infected triatomines were characterized molecular ... | 2008 | 18784237 |
| urban transmission of chagas disease in cochabamba, bolivia. | chagas disease is a major public health problem in bolivia. in the city of cochabamba, 58% of the population lives in peripheral urban districts ("popular zones") where the infection prevalence is extremely high. from 1995 to 1999, we studied the demographics of chagas infections in children from five to 13 years old (n = 2218) from the south zone (sz) and north zone (nz) districts, which differ in social, environmental, and agricultural conditions. information gathered from these districts demo ... | 2008 | 18797753 |
| impregnated netting slows infestation by triatoma infestans. | we used sentinel animal enclosures to measure the rate of infestation by the chagas disease vector, triatoma infestans, in an urban community of arequipa, peru, and to evaluate the effect of deltamethrin-impregnated netting on that rate. impregnated netting decreased the rate of infestation of sentinel enclosures (rate ratio, 0.23; 95% confidence interval, 0.13-0.38; p < 0.001), controlling for the density of surrounding vector populations and the distance of these to the sentinel enclosures. mo ... | 2008 | 18840739 |
| comparison of feeding and defecation patterns between fifth-instar nymphs of triatoma patagonica (del ponte, 1929) and triatoma infestans (klug, 1934) under laboratory conditions. | among the vectors of chagas disease, triatoma patagonica is a species in the process of adaptation to the human environment. however, its vector competence is not well known. this study had the aim of evaluating and comparing feeding and defecation patterns among fifth-instar nymphs of triatoma patagonica and triatoma infestans that were fed ad libitum. the results showed that nymphs of triatoma patagonica had a feeding pattern similar to that of triatoma infestans. sixty nine percent and 58% of ... | 2008 | 18853002 |
| a new method for forensic dna analysis of the blood meal in chagas disease vectors demonstrated using triatoma infestans from chuquisaca, bolivia. | feeding patterns of the vector are important in the epidemiology of chagas disease, the leading cause of heart disease in latin america. chagas disease is caused by the parasite, trypanasoma cruzi, which is transmitted by blood feeding insects. historically, feeding behaviours of haematophagous insects have been investigated using serological reactions, which have detection limits in terms of both taxonomic resolution, and quantity and quality of the blood meal. they are labor intensive, require ... | 2008 | 18974787 |
| suitability of a rapid dna isolation and amplification for detection of trypanosoma cruzi in triatoma infestans dry fecal spots collected on filter paper. | a rapid dna extraction was used for t. cruzi detection in triatomines dry fecal spots collected on filter paper and analyzed by pcr. fifty t. infestans were fed on experimentally infected balb/c mice with high t. cruzi parasitemia and divided into five groups of ten triatomines, and 100 triatomines were infected with lower parasitemia and divided into five groups of 20 triatomines. one dry fecal spot was analyzed per group on days 1, 2, 3, 4 and 5 post feeding. amplification targeted t. cruzi tc ... | 2008 | 19202767 |
| further evidence of spontaneous cure in human chagas disease. | an acute case of chagas disease was studied in 1944, with clinical and laboratory follow-up until 2007, in bambuí, minas gerais, brazil. a five-year-old girl living in a rural hut that was highly infested with triatoma infestans presented a febrile clinical condition compatible with the acute form of trypanosomiasis. she presented a positive thick blood smear, but never again showed serological and/or parasitological evidence of trypanosoma cruzi infection, on several occasions. this patient nev ... | 2008 | 19009195 |
| macrogeographic and microgeographic genetic structure of the chagas' disease vector triatoma infestans (hemiptera: reduviidae) from catamarca, argentina. | the genetic structure in populations of the chagas' disease vector triatoma infestans from six localities belonging to areas under the same insecticide treatment conditions of catamarca province (argentina) was examined at macrogeographical and microgeographical scales. a total of 238 insects were typed for 10 polymorphic microsatellite loci. the average observed and expected heterozygosities ranged from 0.319 to 0.549 and from 0.389 to 0.689, respectively. the present results confirm that popul ... | 2008 | 17885811 |
| the elimination of chagas' disease from brazil. | on 9 june 2006 the pan american health organization (paho) presented the minister of health of brazil with the international elimination of transmission of chagas' disease certificate. this act was the culmination of an intensive process that began in 1991 with the southern cone initiative, a joint agreement between the governments of argentina, bolivia, brazil, chile, paraguay, uruguay and peru, to control chagas' disease by the elimination of the main vector, triatoma infestans. this initiativ ... | 2008 | 18053273 |
| volatile compounds secreted by brindley's glands of adult triatoma infestans: identification and biological activity of previously unidentified compounds. | volatile emissions of adult male triatoma infestans were collected on non-polar spme fibers and analyzed by gas chromatography linked to a mass spectrometer. a complex mixture of 16 short-chain esters and acids were identified. the composition of short-chain aliphatic acids (ethanoic to nonanoic acids) was similar to previously reported results. the most abundant aliphatic acid was 2-methylpropanoic acid, constituting 18% of the total volatile content. also abundant were the esters 2- and 3-meth ... | 2007 | 17633428 |
| mating behavior of the hematophagous bug triatoma infestans: role of brindley's and metasternal glands. | we investigated if brindley's and metasternal glands are involved in the sexual behavior of triatoma infestans. in laboratory assays, we analyzed the effect of selective occlusion of brindley's and metasternal glands of the female (separately and together) on the behavior of males. control assays without occlusion of glands were also performed. we quantitatively tested if such glands affect mating occurrence, the copulatory attempts of males, and the aggregation of males around a mating couple. ... | 2007 | 17509610 |
| distribution and characterization of nitric oxide synthase in the nervous system of triatoma infestans (insecta: heteroptera). | the biochemical characterization of nitric oxide synthase (nos) and its distribution in the central nervous system (cns) were studied in the heteropteran bug triatoma infestans. nos-like immunoreactivity was found in the brain, subesophageal ganglion, and thoracic ganglia by using immunocytochemistry. in the protocerebrum, nos-immunoreactive (ir) somata were detected in the anterior, lateral, and posterior soma rinds. in the optic lobe, numerous immunostained somata were observed at the level of ... | 2007 | 17235602 |
| identification and characterization of plasma kallikrein-kinin system inhibitors from salivary glands of the blood-sucking insect triatoma infestans. | two plasma kallikrein-kinin system inhibitors in the salivary glands of the kissing bug triatoma infestans, designated triafestin-1 and triafestin-2, have been identified and characterized. reconstitution experiments showed that triafestin-1 and triafestin-2 inhibit the activation of the kallikrein-kinin system by inhibiting the reciprocal activation of factor xii and prekallikrein, and subsequent release of bradykinin. binding analyses showed that triafestin-1 and triafestin-2 specifically inte ... | 2007 | 17645545 |
| differential characterization of holocentric chromosomes in triatomines (heteroptera, triatominae) using different staining techniques and fluorescent in situ hybridization. | a comparative study of holocentric chromosomes in the triatomine species panstrongylus megistus, rhodnius pallescens and triatoma infestans was carried out in order to characterize heterochromatin, rdna active sites and nucleolar proteins. cytological preparations of seminiferous tubules were stained by silver impregnation, c banding, fluorochromes cma3/da and dapi/da, and fluorescent in situ hybridization (fish) with drosophila melanogaster 28s rdna probe. our results showed interesting aspects ... | 2007 | 18050092 |
| allatotropin-like peptide released by malpighian tubules induces hindgut activity associated with diuresis in the chagas disease vector triatoma infestans (klug). | haematophagous insects incorporate a large amount of blood with each meal, producing a big quantity of urine in a few hours to eliminate the excess water and na(+). malpighian tubules (mts) have traditionally been seen as a system that responds to neuroendocrine stimulus. in a related paper, we demonstrated that mts of triatoma infestans produce an autonomous endocrine secretion of an allatotropin-like (at-like) peptide. in the present study, we report a myostimulatory activity of at at the leve ... | 2007 | 17515423 |
| cuticular hydrocarbons of triatomines. | triatomine insects (hemiptera) are the vectors of chagas disease. their cuticular surface is covered by a thin layer of lipids, mainly hydrocarbons, wax esters, fatty alcohols, and free or esterified fatty acids. these lipids play a major role in preventing a lethal desiccation, altering the absorption of chemicals and microorganism penetration, they also participate in chemical communication events. lipid components are biosynthetically related, the synthesis of long chain and very long chain f ... | 2007 | 17046303 |
| daily rhythm of aggregation in the haematophagous bug triatoma infestans (heteroptera: reduviidae). | triatomine bugs show a temporal modulation of many activities. here, we analyse the daily modulation of the aggregation behaviour of triatoma infestans larvae and its chronobiological basis. in the laboratory, groups of six bugs were released over an experimental arena during six consecutive days, where their aggregation behaviour was quantified every hour. when submitted to a 12/12 h photoperiod (l/d), the larvae of t. infestans exhibited a cyclic pattern of aggregation with a 24 h period, evin ... | 2007 | 17612764 |
| impact of community-based vector control on house infestation and trypanosoma cruzi infection in triatoma infestans, dogs and cats in the argentine chaco. | the relative impact of two community-based vector control strategies on house infestation by triatoma infestans and trypanosoma cruzi infection in bugs, domestic dogs and cats was assessed in two neighboring rural areas comprising 40 small villages and 323 houses in one of the regions most endemic for chagas disease in northern argentina. the prevalence and abundance of domestic infestation were 1.5- and 6.5-fold higher, respectively, in the area under pulsed, non-supervised control actions oper ... | 2007 | 17686448 |
| re-establishment of local populations of vectors of chagas disease after insecticide spraying. | 1. prevention of chagas disease is mainly dependent on control of the insect vectors that transmit infection. unfortunately, this control is not wholly successful and the vectors have been resurgent in some areas. where re-infestation has occurred, it is important to understand the dynamics of the process. we investigated how a metapopulation framework can elucidate key aspects of re-infestation and thereby contribute to more efficient disease control.2.triatoma infestans, the main vector of cha ... | 2007 | 17710182 |
| southern cone initiative for the elimination of domestic populations of triatoma infestans and the interruption of transfusional chagas disease. historical aspects, present situation, and perspectives. | created in 1991 by the governments of argentina, bolivia, brazil, chile, paraguay, and uruguay, the southern cone initiative (sci) has been extremely important for chagas disease control in this region. its basic objective was to reach the interruption of this disease, chiefly by means of the elimination of the principal vector triatoma infestans and by the selection of safe donors in the regional blood banks. after a summarized historic of sci, the text shows the advance of technical and operat ... | 2007 | 17891281 |
| susceptibility of mepraia spinolai and triatoma infestans to different trypanosoma cruzi strains from naturally infected rodent hosts. | trypanosoma cruzi is the causative agent of chagas disease, a zoonosis involving domestic and sylvatic mammalian reservoirs. since scarce information has been published about the susceptibility of t. cruzi lineages to other triatomine species besides triatoma infestans, we evaluate the susceptibility of t. infestans and mepraia spinolai to different t. cruzi lineages, originated from naturally infected octodon degus rodents as mammal host. xenodiagnosis-pcr methods to detect t. cruzi positive ro ... | 2007 | 17904090 |
| sustainable vector control and management of chagas disease in the gran chaco, argentina. | chagas disease remains a serious obstacle to health and economic development in latin america, especially for the rural poor. we report the long-term effects of interventions in rural villages in northern argentina during 1984-2006. two community-wide campaigns of residual insecticide spraying immediately and strongly reduced domestic infestation and infection with trypanosoma cruzi in triatoma infestans bugs and dogs and more gradually reduced the seroprevalence of children <15 years of age. be ... | 2007 | 17913895 |
| a method for the identification of guinea pig blood meal in the chagas disease vector, triatoma infestans. | in a sine-based pcr assay, a primer set specific for guinea pig genome short interspersed elements dna was used to test the utility of genomic markers for identifying the source of vertebrate blood meals of triatoma infestans. | 2007 | 17222347 |
| infestation of rural houses by triatoma infestans in the region of los llanos (la rioja, argentina). | vectorial transmission of chagas disease has been strongly reduced in most parts of the southern cone countries of south america, except in the gran chaco region of argentina, bolivia, and paraguay. given periodical interruptions of the vector control programmes in the endemic region of the gran chaco of argentina, the vectorial transmission of the disease has been increasing during the last years. from the beginning of 2004, the provincial ministry of health of la rioja, argentina, started a ve ... | 2007 | 17294001 |
| trypanosoma cruzi detection in blood by xenodiagnosis and polymerase chain reaction in the wild rodent octodon degus. | we detected trypanosoma cruzi in blood samples of the wild rodent octodon degus by xenodiagnosis and a polymerase chain reaction (pcr) using the domestic and wild vectors of chagas disease, triatoma infestans and mepraia spinolai, respectively. we captured 35 rodents and extracted dna from blood samples and intestinal contents of vectors fed on o. degus. our results indicate that the percentage of rodents naturally infected with t. cruzi depends on the biologic sample used for pcr and on the vec ... | 2007 | 17297043 |
| vaccination with trypanosoma rangeli reduces the infectiousness of dogs experimentally infected with trypanosoma cruzi. | the goal of this work was to test the efficacy of the vaccination with trypanosoma rangeli in dogs. mongrel dogs received three subcutaneous injections of fixed t. rangeli epimastigotes at 6-week intervals. such immunisation induced antibodies against trypanosoma cruzi. while both control and immunised dogs developed detectable parasitemia, this was lower and shorter in vaccinated animals. interestingly, feeding of triatoma infestans nymphs on vaccinated and chronically infected dogs led to a sh ... | 2007 | 17349724 |
| microsatellite analysis of genetic structure in natural triatoma infestans (hemiptera: reduviidae) populations from argentina: its implication in assessing the effectiveness of chagas' disease vector control programmes. | the genetic structure in populations of the chagas' disease vector triatoma infestans was examined. comparisons of the levels of genetic variability in populations of this species from areas with different periods since last insecticide treatment and from areas that never received treatment were also carried out. a total of 598 insects from 19 populations were typed for 10 polymorphic microsatellite loci. the average observed and expected heterozygosities ranged from 0.186 to 0.625 and from 0.17 ... | 2007 | 17391265 |
| sylvatic triatoma infestans (reduviidae, triatominae) in the andean valleys of bolivia. | triatoma infestans is the main vector of chagas disease in the southern cone countries. wild populations of t. infestans appear widespread throughout the andean valleys of bolivia. in cotapachi (2750 m asl), all sorts of rocky outcrops, regardless of their size, provided good refuges for t. infestans. of the 1120 ecotopes investigated, 330 (29.5%) contained triatomines and 92% of the collected insects were nymphal instars. in the cold season, triatomine densities were similar in small and large ... | 2007 | 17397789 |
| flight muscle dimorphism and heterogeneity in flight initiation of field-collected triatoma infestans (hemiptera: reduviidae). | recent experiments demonstrated that most field-collected triatoma infestans (klug) (hemiptera: reduviidae) adults from northern argentina either never initiated flight or did so repeatedly in both sexes. this pattern could not be explained by sex, adult age, weight, weight-to-length ratio (w/l), or chance. we examined whether bugs that never initiated flight possessed developed flight muscles, and whether flight muscle mass relative to total body mass (fmr) was related to the probability of fli ... | 2007 | 17427685 |
| acid phosphatase activity distribution in salivary glands of triatomines (heteroptera, reduviidae, triatominae). | acid phosphatase activity (gömori technique) in salivary gland cells was investigated in adult insects (males and females) of four species of triatomines: triatoma infestans, panstrongylus megistus, rhodnius neglectus, and rhodnius prolixus. binucleated cells with bulky and polyploidy nuclei were detected, with acid phosphatase activity in the heterochromatin and nucleolus, which showed the most intense response. thus, the activity of these phosphatases during rrna molecule transcription, possib ... | 2007 | 17469069 |
| evidence of congenital transmission of trypanosoma cruzi in a vector-free area of bolivia. | a seroprevalence survey of trypanosoma cruzi was carried out in two areas of south bolivia. triatoma infestans, the main vector of tryp. cruzi, was abundant in the first area, but absent in the second one. titration of tryp. cruzi antibodies was carried out in children aged 6-24 months and their mothers. the seroprevalence of chagas' disease was significantly higher in the area with the vector, but also high in the second area. chagas' infection in children under 2 years old could be linked to c ... | 2007 | 17499827 |
| active dispersal by wild triatoma infestans in the bolivian andes. | triatoma infestans is the main vector of chagas disease and target of control programmes in the southern cone countries. so far bolivia is the only country where true t. infestans wild foci are documented. the dispersal ability for wild t. infestans was studied at microgeographical scale in bolivian andes, to assess the possibility for wild populations to actively recolonize insecticide-treated villages. nine microsatellite loci were used to detect the extent of gene flow between neighbouring co ... | 2007 | 17550473 |
| the detection of trypanosoma cruzi in triatoma infestans: comparison of a pcr-based assay with microscopical examination. | 2007 | 17550653 | |
| [geographical distribution and indicators entomologic of sinantropic triatomines captured in the state of goiás]. | the geographic distribution of triatomines in goiás and entomological indicators in the home environment are presented: home infestation, home triatomine density and vector infection by trypanosoma cruzi. the indicators studied were from triatomines captured inside and outside homes in 201 municipalities in the state of goiás, brazil, between 2000 and 2003. a total of 249,868 home units were investigated and 51,570 triatomines were captured, and 335 specimens were found to be infected with trypa ... | 2007 | 17568889 |
| pcr reveals significantly higher rates of trypanosoma cruzi infection than microscopy in the chagas vector, triatoma infestans: high rates found in chuquisaca, bolivia. | the andean valleys of bolivia are the only reported location of sylvatic triatoma infestans, the main vector of chagas disease in this country, and the high human prevalence of trypanosoma cruzi infection in this region is hypothesized to result from the ability of vectors to persist in domestic, peri-domestic, and sylvatic environments. determination of the rate of trypanosoma infection in its triatomine vectors is an important element in programs directed at reducing human infections. traditio ... | 2007 | 17597541 |
| domestic dogs and cats as sources of trypanosoma cruzi infection in rural northwestern argentina. | the reservoir capacity of domestic cats and dogs for trypanosoma cruzi infection and the host-feeding patterns of domestic triatoma infestans were assessed longitudinally in 2 infested rural villages in north-western argentina. a total of 86 dogs and 38 cats was repeatedly examined for t. cruzi infection by serology and/or xenodiagnosis. the composite prevalence of infection in dogs (60%), but not in cats, increased significantly with age and with the domiciliary density of infected t. infestans ... | 2007 | 17032467 |
| biochemistry of insect epicuticle degradation by entomopathogenic fungi. | the biochemical interaction between fungal pathogens and their insect host epicuticle was studied by examining fungal hydrocarbon degrading ability. as a contact insecticide, entomopathogenic fungi invade their host through the cuticle, covered by an outermost lipid layer mainly composed of highly stable, very long chain structures. strains of beauveria bassiana and metarhizium anisopliae (deuteromycotina: hyphomycetes), pathogenic both to the blood-sucking bug triatoma infestans (hemiptera: red ... | 2007 | 17052960 |
| the pcr-based detection of trypanosoma cruzi in the faeces of triatoma infestans fed on patients with chronic american trypanosomiasis gives higher sensitivity and a quicker result than routine xenodiagnosis. | in the xenodiagnosis (xd) of american trypanosomiasis (chagas disease), trypanosoma cruzi in the triatomine bugs fed on the patient can now be detected using pcr (xd-pcr) as well as by microscopy (xd-m). in a study to compare xd-pcr with xd-m, triatomine bugs were fed on 50 cases of chronic american trypanosomiasis, of whom only 25 were ever found positive by xd-m. overall, the bugs fed on 34 of the patients (all 25 cases found positive by xd-m and nine of the other patients) were found pcr-posi ... | 2007 | 18028728 |
| an insight into the sialome of the blood-sucking bug triatoma infestans, a vector of chagas' disease. | triatoma infestans is a hemiptera, vector of chagas' disease that feeds exclusively on vertebrate blood in all life stages. hematophagous insects' salivary glands (sg) produce potent pharmacological compounds that counteract host hemostasis, including anticlotting, antiplatelet, and vasodilatory molecules. to obtain a further insight into the salivary biochemical and pharmacological complexity of this insect, a cdna library from its sg was randomly sequenced. also, salivary proteins were submitt ... | 2007 | 18207082 |
| chemical communication in chagas disease vectors. source, identity, and potential function of volatiles released by the metasternal and brindley's glands of triatoma infestans adults. | compounds from the metasternal and brindley's glands of the blood-sucking bug, triatoma infestans, were identified by solid phase microextraction (spme) and gas chromatography-mass spectrometry. volatile compounds released by adult bugs during copulation or after mechanical disturbance were also characterized. six compounds were identified and found consistently in all samples from metasternal glands. the most abundant were 3-pentanone, 2-methylbutanol, 3-pentanol, and an unidentified compound. ... | 2006 | 16902820 |