Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| anti-neospora caninum antibody detection and vertical transmission rate in pregnant zebu beef cows (bos indicus): neospora caninum in pregnant beef cows (bos indicus). | the aim of the present study was to evaluate anti-neospora caninum antibodies and the vertical transmission rate in naturally infected pregnant zebu beef cows (bos indicus) reared on pasture. the present study began with 200 cows from four farms (50 cows from each farm), and these animals were submitted to timed artificial insemination (tai). after ultrasonography, 76 pregnant cows were selected, 22, 15, 22, and 17, respectively, from farms 1, 2, 3, and 4. blood samples were taken from cows thri ... | 2014 | 25193073 |
| toxoplasma gondii and neospora caninum infections in goat abortions from argentina. | the aims of this study were to identify the occurrence of toxoplasma gondii and neospora caninum abortions in goats from argentina by serological, macroscopical and microscopical examination and bioassay, and to characterize the obtained isolates by molecular techniques. for this purpose, 25 caprine fetal fluids, 18 caprine fetal brains and 10 caprine placentas from 8 dairy/meat goat farms from argentina were analyzed. gestational age of the aborted fetuses was determined in 18 cases. protozoal ... | 2014 | 25128663 |
| the chloramphenicol acetyltransferase vector as a tool for stable tagging of neospora caninum. | neospora caninum is an obligate intracellular apicomplexa, a phylum where one of the current methods for functional studies relies on molecular genetic tools. for toxoplasma gondii, the first method described, in 1993, was based on resistance against chloramphenicol. as in t. gondii, we developed a vector constituted of the chloramphenicol acetyltransferase gene (cat) flanked by the n. caninum dihydrofolate reductase-thymidylate synthase (dhfr-ts) 5' coding sequence flanking region. five weeks a ... | 2014 | 25127750 |
| fluorescent ester dye-based assays for the in vitro measurement of neospora caninum proliferation. | techniques for the measurement of parasite loads in different experimental models have evolved throughout the years. the quantification of stained slides using regular cytological stains is currently the most common technique. however, this modality of evaluation is labor-intensive, and the interpretation of the results is subjective because the successes of the assays mainly rely on the abilities of the professionals involved. moreover, the novel genetic manipulation techniques that are commonl ... | 2014 | 25095733 |
| cytokine expression in the placenta of pregnant cattle after inoculation with neospora caninum. | neospora caninum is recognized as a major cause of reproductive losses worldwide but its pathogenesis is not completely understood. immune mediated placental pathology has been reported as being responsible for compromising pregnancy probably due to the adverse effects of exacerbated th1 type response at the maternal-foetal interface. different clinical outcomes are known to occur following experimental infections of cattle at different stages of gestation, with foetal death being the most commo ... | 2014 | 25091332 |
| occurrences of anti-toxoplasma gondii and anti-neospora caninum antibodies in barbary sheep at curitiba zoo, southern brazil. | barbary sheep (ammotragus lervia) have the potential to act as hosts of important infectious diseases, particularly zoonoses. blood samples from 17 barbary sheep at the curitiba zoo were collected to evaluate occurrences of anti-toxoplasma gondii and anti-neospora caninum antibodies, tested using the indirect immunofluorescence antibody test (ifat). anti-t. gondii and anti-n. caninum antibodies were detected in 4/17 (23.5%) and 4/17 (23.5%) samples, respectively. the present study has shown that ... | 2014 | 25054509 |
| comparison of host cell invasion and proliferation among neospora caninum isolates obtained from oocysts and from clinical cases of naturally infected dogs. | in a previous study we have shown that the in vitro invasion rate (ir) and tachyzoite yield (ty) are associated with the virulence phenotypes of neospora caninum isolates of bovine origin. in addition, we recently observed marked differences in virulence when canine isolates were compared in a pregnant balb/c mouse model. in this study, we investigated whether invasion and proliferation capacities could be used as virulence-related n. caninum phenotypic traits. of the isolates compared in mice, ... | 2014 | 25045851 |
| discovering a vaccine against neosporosis using computers: is it feasible? | a vaccine is urgently needed to prevent cattle neosporosis. this infectious disease is caused by the parasite neospora caninum, a complex biological system with multifaceted life cycles. an in silico vaccine discovery approach attempts to transform digital abstractions of this system into adequate knowledge to predict candidates. researchers need current information to implement such an approach, such as understanding evasion mechanisms of the immune system, type of immune response to elicit, av ... | 2014 | 25028089 |
| toxoplasmosis-associated abortion in an alpaca (vicugna pacos) fetus. | a near-full-term alpaca (vicugna pacos) was stillborn 2 days before the expected date of delivery; necropsy examination was conducted within 6 hr of delivery. gross lesions were enlarged liver and hydrocephalus. on histologic examination, mild inflammatory lesions were identified in the placenta, liver, and lungs, although no etiology was recognized. within the brain, there was a mild nonsuppurative meningoencephalitis, hydrocephalus, and hydromyelia. both kidneys had inflammatory foci in cortex ... | 2014 | 25000722 |
| diagnosis and treatment of neospora caninum--associated dermatitis in a red fox (vulpes vulpes) with concurrent toxoplasma gondii infection. | a 3-mo-old red fox (vulpes vulpes) developed generalized crusty plaques on its body during rehabilitation after an automobile accident requiring amputation of one leg. histologic examination of skin lesion biopsy revealed granulomatous dermatitits with many intralesional protozoal tachyzoites. the protozoa stained positively with antibodies to neospora caninum but not to toxoplasma gondii. treatment with clindamycin hydrochloride (10 mg/kg, twice daily, s.c.) for 1 mo completely resolved lesions ... | 2014 | 25000720 |
| neosporosis in naturally infected pregnant dairy cattle. | neosporosis caused by caused by the apicomplexan parasite neospora caninum is one of the major causes of infectious abortion in bovines worldwide. a long-term prospective study was performed in a dairy herd endemic for n. caninum in order to analyze the impact of neosporosis on the proportion of aborting cows. a total of 1078 pregnant cows were tested for presence of antibodies and the proportion of abortions was calculated. the overall seroprevalence of n. caninum found in the herd was 35.5%. t ... | 2014 | 24986462 |
| metabolic footprinting of extracellular metabolites of brain endothelium infected with neospora caninum in vitro. | the survival of the intracellular protozoan parasite neospora caninum depends on its ability to adapt to changing metabolic conditions of the host cell. thus, defining cellular and metabolic changes in affected target tissues may aid in delineating pathogenetic mechanism. we undertook this study to assess the metabolic response of human brain microvascular endothelial cells (hbmecs) to n. caninum infection in vitro. | 2014 | 24973017 |
| prevalence and correlates of antibodies to neospora caninum in dogs in portugal. | neosporosis, caused by neospora caninum, is an important cause of abortion in cattle and of neurological disease in dogs. this study investigated the prevalence and correlates of antibodies to n. caninum in 441 dogs from the five regions of mainland portugal. a commercial competitive enzyme-linked immunosorbent assay (celisa) was used and specific antibodies were detected in 35 (7.9%) dogs. seroprevalence levels were significantly different among some of the studied regions, as well as between s ... | 2014 | 24972327 |
| two distinct populations of bovine il-17⁺ t-cells can be induced and wc1⁺il-17⁺γδ t-cells are effective killers of protozoan parasites. | il-17 has emerged as a key player in the immune system, exhibiting roles in protection from infectious diseases and promoting inflammation in autoimmunity. initially thought to be cd4 t-cell-derived, the sources of il-17 are now known to be varied and belong to both the innate and adaptive arms of the immune system. mechanisms for inducing il-17 production in lymphoid cells are thought to rely on appropriate antigenic stimulation in the context of tgf-β1, il-6 and/or il-1β. using culture protoco ... | 2014 | 24961164 |
| neospora caninum prevalence in dogs raised under different living conditions. | neospora caninum is an important cause of abortion in dairy cattle worldwide. dogs are important in the epidemiology of n. caninum because they act as definitive hosts shedding oocysts in the environment. vertical transmission of the parasite is well recognized as an important aspect of the epidemiology of the parasite but the importance of horizontal transmission has been less studied. a n. caninum competitive elisa was used to examine serum samples from 600 dogs that were raised under 4 differ ... | 2014 | 24957000 |
| first isolation of neospora caninum from blood of a naturally infected adult dairy cow in beijing, china. | neospora caninum is an important cause of abortion in cattle worldwide, but the isolation of a viable parasite from an abortus is difficult, and viable n. caninum has not been isolated from any host in china. in the present study, peripheral blood samples were collected from a jugular vein of an adult dairy cow that had aborted; the cow was seropositive to n. caninum antibodies by elisa. white blood cells were separated and seeded onto vero cell monolayer cultures for parasite isolation. tachyzo ... | 2014 | 24945568 |
| rop18 is a key factor responsible for virulence difference between toxoplasma gondii and neospora caninum. | toxoplasma gondii (t. gondii) and neospora caninum (n. caninum) are both obligate intracellular protozoan parasites and share many common morphological and biological features. despite these similarities the two parasites differ dramatically in virulence in mice, but the factors involved in virulence differences between the two parasites remain unknown. a secreted serine-threonine kinase called rhoptry protein 18 (rop18) was identified to play a crucial role on virulence differences among differ ... | 2014 | 24927100 |
| experimental ruminant models for bovine neosporosis: what is known and what is needed. | at present, bovine neosporosis is an important worldwide concern because of its wide geographic distribution and economic impact. abortion is the main clinical sign of bovine neosporosis in both dairy and beef cattle. ruminant challenge models are critical to evaluate potential vaccine candidates to help tackle bovine neosporosis and to study pathogenesis and host responses to infection. several research groups have developed ruminant models of neospora caninum infection independently of others, ... | 2014 | 24926962 |
| genetic characterisation of neospora caninum strains from clinical samples of zebuine foetuses obtained in abattoirs in goiás, brazil. | the neospora caninum microsatellite markers were applied to clinical samples. genotyping technology involving fluorescently labelled dna fragment analysis was used in combination with dna sequencing for markers with complex repetitive sequences. nineteen dna samples from 15 brains and four hearts of naturally infected non-aborted zebuine foetuses from abattoirs in goiás, brazil. n. caninum had been detected in these foetuses by nested-pcr of the internal transcribed spacer-1 rrna region, and the ... | 2014 | 24893690 |
| maternal and fetal immune response patterns in heifers experimentally infected with neospora caninum in the second trimester of pregnancy--a descriptive study. | fetal and maternal immune responses 3, 6 and 9 weeks post infection (wpi) were investigated in cows experimentally infected with neospora caninum on day 110 of gestation. descriptive analysis showed that the fetuses had lower percentages of spleen t cell subpopulations (cd3+, cd4+ and cd8+) at 6 wpi compared to 3 wpi and/or 9 wpi, with the lowest percentages observed in a dead fetus found upon euthanasia at that time. increased expression of most cytokines over levels recorded at 3 and 9 wpi wer ... | 2014 | 24880649 |
| macrophage depletion prior to neospora caninum infection results in severe neosporosis in mice. | we observed that murine macrophages showed greater activation and increased interleukin 6 (il-6), il-12p40, and interferon gamma (ifn-γ) production during neospora caninum infection. many macrophages migrated to the site of infection. furthermore, macrophage-depleted mice exhibited increased sensitivity to n. caninum infection. this study indicates that macrophages are required for achieving protective immunity against n. caninum. | 2014 | 24872515 |
| statistical evaluation of a commercial neospora caninum competitive elisa in the absence of a gold standard: application to wild elk (cervus elaphus) in alberta. | neospora caninum is an important abortive agent of domestic ruminants, but few diagnostic tools are available to reliably assess the exposure of wild cervid species such as elk (cervus elaphus) to this pathogen, which limits our ability to understand their role in the life cycle of this parasite. in the absence of a gold standard test or panels of samples from individuals of known infection status, classical laboratory-based validation methods are not applicable. however, there are a number of s ... | 2014 | 24861013 |
| epidemiology of leishmania infantum, toxoplasma gondii, and neospora caninum in rattus rattus in absence of domestic reservoir and definitive hosts. | isolated environments are privileged settings to study transmission of infection. montecristo is a small island where no wild or domestic carnivores are present. invasive black rats rattus rattus (n=78) were captured and tested by pcr for leishmania infantum, toxoplasma gondii and neospora caninum. we wanted to test, for these parasites, the existence of a sylvatic cycle independent of reservoir or definitive hosts. none of the rats tested positive by pcr for either t. gondii or n. caninum. we r ... | 2014 | 24295953 |
| prevalence of immunoglobulin g (igg) antibody to neospora caninum in dairy cattle of hamedan province, west of iran. | bovine neosporosis caused by the apicomplexan protozoan parasite n. caninum, was initially recognized in 1989 and is now reported as a leading infectious cause of reproductive failure in dairy cattle in world wide. the aim of this study was to determine the seroprevalence of n. caninum infection in industrial dairy cattle of hamedan province (west of iran) by elisa method. blood samples were collected from 492 cattle in 41 farms. antibodies to n. caninum were found in 63(12.80%) sera. a signific ... | 2014 | 25568710 |
| seroprevalence of neospora caninum in slaughtered native cattle in kurdistan province, iran. | neospora caninum is a worldwide distributed pathogen which causes abortion in cattle leading to economic loss in the cattle industry. the aim of this study was to determine the seroprevalence of n. caninum antibodies in the native cattle slaughtered in various areas of kurdistan province (western iran) from september 2010 to september 2011. serum samples from 368 cattle slaughtered in seven slaughterhouses in this region were taken for detection of anti-n. caninum antibodies using commercial n. ... | 2014 | 25568696 |
| isolation, identification, and pathogenicity of neospora caninum china yanbian strain. | the aim of the study was to provide a point of reference to study the neospora caninum infections in china. | 2014 | 25678924 |
| differential locus expansion distinguishes toxoplasmatinae species and closely related strains of toxoplasma gondii. | toxoplasma gondii is a human obligate intracellular parasite that has infected over 20% of the world population and has a vast intermediate host range compared to those of its nearest relatives neospora caninum and hammondia hammondi. while these 3 species have highly syntenic genomes (80 to 99%), in this study we examined and compared species-specific structural variations, specifically at loci that have undergone local (i.e., tandem) duplication and expansion. to do so, we used genomic sequenc ... | 2014 | 24496792 |
| management practices as risk factors for the presence of bulk milk antibodies to salmonella, neospora caninum and leptospira interrogans serovar hardjo in irish dairy herds. | a survey of management practices in 309 irish dairy herds was used to identify risk factors for the presence of antibodies to salmonella, neospora caninum and leptospira interrogans serovar hardjo in extensively managed unvaccinated dairy herds. a previous study documented a herd-level seroprevalence in bulk milk of 49%, 19% and 86% for salmonella, neospora caninum and leptospira interrogans serovar hardjo, respectively in the unvaccinated proportion of these 309 herds in 2009. association analy ... | 2014 | 24661904 |
| detection of neospora caninum-dna in feces collected from dogs in shenyang (china) and its1 phylogenetic analysis. | neospora caninum is an intracellular protozoan that infects many domestic and wild animals. dog is known as a definitive host of n. caninum and involved in transmitting infections to intermediate hosts by shedding oocysts. to investigate the epidemiology of dog neosporosis in china, 212 dog feces specimens in shenyang were screened by nested-pcr using nc5 primers and confirmed by n. caninum its1 pcr. the positive rate of n. caninum dna was 34.90% (74/212). there were no significant correlations ... | 2014 | 25047704 |
| a neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge. | the aim of the present study was to evaluate the immunogenicity and protective efficacy of rncsag1, rnchsp20 and rncgra7 recombinant proteins formulated with immune stimulating complexes (iscoms) in pregnant heifers against vertical transmission of neospora caninum. twelve pregnant heifers were divided into 3 groups of 4 heifers each, receiving different formulations before mating. immunogens were administered twice subcutaneously: group a animals were inoculated with three recombinant proteins ... | 2014 | 25467890 |
| cloning and expression of neospora caninum dense-granule 7 in e. coli. | neospora caninum is an obligate intracellular protozoan which induces abortion, still birth and neuromuscular disorders in cattle and is an important problem in dairy and beef industry worldwide. the dense granule protein 7 (gra7) of n. caninum is an immune-dominant protein shared by both tachyzoite and bradyzoites. this study was conducted to produce recombinant gra7 of n. caninum using a plasmid with high level of expression of this protein in e. coli. for this purpose, a segment of n. caninum ... | 2014 | 24808652 |
| epidemiology of reproductive pathogens in semi-intensive lamb-producing flocks in north-west spain: a comparative serological study. | the aim of this study was to better understand the epidemiology of five major reproductive pathogens in semi-intensive lamb-producing flocks in north-west spain. two thousand four hundred serum samples were collected from 44 flocks, and several factors were studied to assess their influence on seropositivity. farms that tested seropositive for more than one pathogen were common (84.1%), but seroprevalence was high only for toxoplasma gondii (38.1%), and low for the other four pathogens, namely, ... | 2014 | 24685472 |
| evaluation of spore wall protein 1 as an alternative antigen for the diagnosis of encephalitozoon cuniculi infection of farmed foxes using an enzyme-linked immunosorbent assay. | the sequence encoding swp1 was cloned from the genome of encephalitozoon cuniculi. recombinant swp1 (rswp1) was expressed in escherichia coli and used to detect e. cuniculi infections in farmed foxes and dogs with an indirect enzyme-linked immunosorbent assay (elisa) in the present study. the sera of foxes infected with e. cuniculi could be distinguished from the sera of foxes infected with toxoplasma gondii, neospora caninum, and cryptosporidium parvum using the elisa. in total, 198 fox samples ... | 2014 | 24830821 |
| using open-access taxonomic and spatial information to create a comprehensive database for the study of mammalian and avian livestock and pet infections. | what are all the species of pathogen that affect our livestock? as 6 out of every 10 human pathogens came from animals, with a good number from livestock and pets, it seems likely that the majority that emerge in the future, and which could threaten or devastate human health, will come from animals. only 10 years ago, the first comprehensive pathogen list was compiled for humans; we still have no equivalent for animals. here we describe the creation of a novel pathogen database, and present outp ... | 2014 | 23906393 |
| pathogens of zoonotic and biological importance in roe deer (capreolus capreolus): seroprevalence in an agro-system population in france. | antibody prevalence for several infectious and parasitic diseases in a population of roe deer (capreolus capreolus) inhabiting a mixed agricultural landscape (south of france) has been analyzed. serological analyses with elisa in 245 animals captured from 2008 to 2012 has been performed. we found a high prevalence of toxoplasma gondii (46.4%), chlamydophila abortus (17.27%) and coxiella burnetii (11.26%) compared to other studies in europe. seroprevalence varied strongly among years for t. gondi ... | 2014 | 24576494 |
| neospora caninum infection as a cause of reproductive failure in a sheep flock. | neospora caninum has been detected only sporadically in cases of ovine abortion, and it has therefore traditionally been considered as an unimportant parasite in small ruminants. this study was carried out with the aim of identifying the pathogen causing serious reproductive problems on a commercial sheep farm. sera from all rams and ewes tested negative for antibodies against border disease virus, schmallenberg virus and coxiella burnetii, and infections by these agents were therefore ruled out ... | 2014 | 25158756 |
| serosurvey on schmallenberg virus and selected ovine reproductive pathogens in culled ewes from southern spain. | after the first case of schmallenberg virus (sbv) was reported in southern spain (march 2012), a retrospective serological study was carried out in extensive sheep flocks from nearby areas to assess the history of exposure to sbv and other selected ovine reproductive pathogens (chlamydophila abortus, coxiella burnetii, border disease virus 'bdv', toxoplasma gondii and neospora caninum). secondly, the presence of antibodies was investigated in meat juice samples against selected pathogens to vali ... | 2014 | 24219149 |
| abortion in cattle due to infection with staphylococcus lugdunensis. | an aborted fetus of 7 months gestation, the associated placenta, and a single blood sample from the dam were submitted for diagnostic investigation to the diagnostic laboratory of the lombardy and emilia-romagna experimental zooprophylactic institute in parma, italy. the serum was negative for neospora caninum, coxiella burnetii, chlamydophila abortus, bovine herpesvirus 1 (bhv-1), bovine viral diarrhea virus (bvdv), brucella abortus, and brucella melitensis. fetal tissues and placental cotyledo ... | 2014 | 25292193 |
| blood collected on filter paper for wildlife serology: evaluating storage and temperature challenges of field collections. | filter-paper (fp) blood sampling can facilitate wildlife research and expand disease surveillance. previous work indicated that nobuto fp samples from caribou and reindeer (rangifer tarandus subspecies) had comparable sensitivity and specificity to serum samples (≥ 80% for both) in competitive enzyme-linked immunosorbent assays (celisas) for brucella spp., neospora caninum, and west nile virus. the same sensitivity and specificity criteria were met in indirect elisas for brucella spp., bovine he ... | 2014 | 24499329 |
| health impact evaluation of alternative management systems in vicuña (vicugna vicugna mensalis) populations in peru. | to determine the impact of farming over vicuña population in peru, serum samples were collected from 207 vicuñas (126 captive vicuñas and 81 free-ranging vicuñas) and 614 domestic south american camelids (571 alpacas and 43 llamas), in ten andean communities at the salinas y aguada blanca reserve, province of arequipa, southern peru. samples were tested for the presence of leptospirosis, foot and mouth disease (fmd), bovine viral diarrhea (bvd), bovine herpesvirus type 1 (bhv-1), brucellosis, bl ... | 2014 | 24492977 |
| blood collected on filter paper for wildlife serology: detecting antibodies to neospora caninum, west nile virus, and five bovine viruses in reindeer. | we compared nobuto filter paper (fp) whole-blood samples to serum for detecting antibodies to seven pathogens in reindeer (rangifer tarandus tarandus). serum and fp samples were collected from captive reindeer in 2008-2009. sample pairs (serum and fp eluates) were assayed in duplicate at diagnostic laboratories with the use of competitive enzyme-linked immunosorbent assays (celisas) for neospora caninum and west nile virus (wnv); indirect elisa (ielisas) for bovine herpesvirus type 1 (bhv-1), pa ... | 2014 | 24484497 |
| serological cross-reactivity of trypanosoma cruzi, ehrlichia canis, toxoplasma gondii, neospora caninum and babesia canis to leishmania infantum chagasi tests in dogs. | the aim of this study was to evaluate the serological cross-reactivity between leishmania sp. and other canine pathogens. | 2014 | 24603745 |
| the parasitic fauna of the european bison (bison bonasus) (linnaeus, 1758) and their impact on the conservation. part 1. the summarising list of parasites noted. | during the current century, 88 species of parasites have been recorded in bison bonasus. these are 22 species of protozoa (trypanosoma wrublewskii, t. theileri, giardia sp., sarcocystis cruzi, s. hirsuta, s. hominis, s. fusiformis, neospora caninum, toxoplasma gondii, cryptosporidium sp., eimeria cylindrica, e. subspherica, e. bovis, e. zuernii, e. canadensis, e. ellipsoidalis, e. alabamensis, e. bukidnonensis, e. auburnensis, e. pellita, e. brasiliensis, babesia divergens), 4 trematodes species ... | 2014 | 25119348 |
| the gatekeeper residue and beyond: homologous calcium-dependent protein kinases as drug development targets for veterinarian apicomplexa parasites. | specific roles of individual cdpks vary, but in general they mediate essential biological functions necessary for parasite survival. a comparative analysis of the structure-activity relationships (sar) of neospora caninum, eimeria tenella and babesia bovis calcium-dependent protein kinases (cdpks) together with those of plasmodium falciparum, cryptosporidium parvum and toxoplasma gondii was performed by screening against 333 bumped kinase inhibitors (bkis). structural modelling and experimental ... | 2014 | 24927073 |
| infectious pathogens potentially transmitted by semen of the black variety of the manchega sheep breed: health constraints for conservation purposes. | conservation of genetic resources from endangered breeds may be conducted through germinal banks. preservation of healthy samples is paramount to avoid preserving pathogens shed with germinal products. the black variety of manchega sheep (bms), and endangered breed endemic to south-central spain, is the subject of a conservation program; a germinal bank has been recently established. however, several pathogens circulating in bms flocks may be shed with semen and threaten bms preservation. theref ... | 2014 | 25066603 |
| prevalence survey of selected bovine pathogens in water buffaloes in the north region of brazil. | although the largest buffalo herd in the occident is in the north region of brazil, few studies have been conducted to assess the prevalence of selected parasitic diseases in buffalo herd. the present study was therefore conducted to investigate the epidemiological of toxoplasma gondii, neospora caninum, anaplasma marginale, babesia bigemina, and babesia bovis in water buffaloes in the north region of brazil. a total of 4796 buffalo blood samples were randomly collected from five provinces and s ... | 2014 | 24563780 |
| serosurveillance of infectious agents in equines of the central valley of costa rica. | blood samples from 181 equines from the central valley of costa rica were collected in the year 2012 to determine the presence of antibodies against selected infectious agents in horses and to determine the risk factors associated with these agents. the presence of antibodies against equine infectious anemia virus (eiav), equine herpes virus 1 and 4 (ehv-1 and ehv-4), west nile virus (wnv), influenza a virus (iav), equine viral arteritis virus (evav), babesia caballi, theileria equi, neospora ca ... | 2014 | 26623349 |
| survey of toxoplasma gondii and neospora caninum, haemotropic mycoplasmas and other arthropod-borne pathogens in cats from albania. | albania is a country on the western part of the balkan peninsula. the mediterranean climate is favourable for the stable development of many arthropod species, which are incriminated as vectors for various agents. recently, several papers have reported on epidemiological aspects of parasitic diseases including vector-borne disease agents of dogs with zoonotic characteristics in albania. however, data on the epidemiology of feline parasitic and bacterial agents in albania is scarce. | 2014 | 24517118 |
| generation and characterization of the first immortalized alpaca cell line suitable for diagnostic and immunization studies. | raising of alpacas as exotic livestock for wool and meat production and as companion animals is growing in importance in the united states, europe and australia. furthermore the alpaca, as well as the rest of the camelids, possesses the peculiarity of producing single-chain antibodies from which nanobodies can be generated. nanobodies, due to their structural simplicity and reduced size, are very versatile in terms of manipulation and bio-therapeutic exploitation. in fact the biotech companies i ... | 2014 | 25140515 |
| pathogens at the livestock-wildlife interface in western alberta: does transmission route matter? | in southwestern alberta, interactions between beef cattle and free-ranging elk (cervus elaphus) may provide opportunities for pathogen transmission. to assess the importance of the transmission route on the potential for interspecies transmission, we conducted a cross-sectional study on four endemic livestock pathogens with three different transmission routes: bovine viral diarrhea virus and bovine herpesvirus 1 (predominantly direct transmission), mycobacterium avium subsp. paratuberculosis (ma ... | 2014 | 24517283 |
| seroprevalence of neospora caninum antibodies in chicken samples from delta egypt using a recombinant ncsag1 protein-based elisa. | neospora caninum is an obligate intracellular protozoan that causes abortion and economic loss in the cattle industry. this study aimed to estimate the prevalence of anti-n. caninum antibodies in chicken using elisa methods based on the surface antigen 1 of n. caninum (ncsag1t). the overall prevalence of n. caninum in chicken was 15.51%. the seroprevalence was high in qalyoubiya, minufiya, kafr el-shaykh, gharbiya, dakahlia provinces; (34%, 17.39%, 14.75%, 14.29%, and 12.25% respectively). in co ... | 2013 | 23888555 |
| first report of antibodies to neospora caninum in nigerian cattle. | 2013 | 23857393 | |
| gc/ms analysis of high-performance liquid chromatography fractions from sophora flavescens and torilis japonica extracts and their in vitro anti-neosporal effects on neospora caninum. | we analyzed alcoholic extracts of herbs possessing anti-neosporal activity against neospora (n.) caninum. to identify the chemical components of sophora (s.) flavescens and torilis (t.) japonica associated with anti-neosporal activity, specific fractions were isolated by high-performance liquid chromatography (hplc). in vitro activity of the fractions against n. caninum was then assessed. gas chromatography/ mass spectrometry (gc/ms) was used to identify and quantify specific anti-neosporal mole ... | 2013 | 23820198 |
| detection of neospora caninum dna in semen of experimental infected rams with no evidence of horizontal transmission in ewes. | recent reports from new zealand indicate neospora caninum has a possible role in causing abortions in sheep. transmission of n. caninum via semen has been documented in cattle. this study aimed to investigate if horizontal transmission through semen was also possible in sheep. initially, 6-month old crossbred ram lambs (n=32), seronegative to n. caninum, were divided into 4 equal groups. group 1 remained uninoculated whilst the remainder were inoculated with n. caninum tachyzoites intravenously ... | 2013 | 23819894 |
| prevalence and dynamics of antibodies against ncsag1 and ncgra7 antigens of neospora caninum in cattle during the gestation period. | bovine abortion caused by the apicomplexan parasite neospora caninum is a major economic problem in the livestock industry worldwide. our study measured the prevalence and temporal changes in levels of antibodies specific for two n. caninum derived antigens, ncsag1 and ncgra7, to determine an appropriate strategy for serodiagnosis. using an enzyme-linked immunosorbent assay (elisa), blood samples showed that 71 cows out of 129 were positive for anti-ncsag1 antibodies and that only nine cows were ... | 2013 | 23782543 |
| first molecular detection of neospora caninum in european brown bear (ursus arctos). | neospora caninum is an important intracellular protozoan parasite with an affinity to the central nervous system of many animals and a major causative agent of repeated abortions in cattle. in total, 45 muscle, liver, or spleen samples of brown bear sampled in different locations of central slovakia were examined by pcr. genotyping of n. caninum was based on amplification of nc-5 gene and its1 rrna fragments and subsequent sequencing. presence of n. caninum dna was confirmed in 24.4% (11/45) of ... | 2013 | 23770068 |
| molecular cloning and characterization of ncrop2fam-1, a member of the rop2 family of rhoptry proteins in neospora caninum that is targeted by antibodies neutralizing host cell invasion in vitro. | recent publications demonstrated that a fragment of a neospora caninum rop2 family member antigen represents a promising vaccine candidate. we here report on the cloning of the cdna encoding this protein, n. caninum rop2 family member 1 (ncrop2fam-1), its molecular characterization and localization. the protein possesses the hallmarks of rop2 family members and is apparently devoid of catalytic activity. ncrop2fam-1 is synthesized as a pre-pro-protein that is matured to 2 proteins of 49 and 55 k ... | 2013 | 23743240 |
| oligomannose-coated liposome-entrapped dense granule protein 7 induces protective immune response to neospora caninum in cattle. | neospora caninum is an intracellular protozoan parasite that causes abortion in cows. vaccination is an important strategy for control of neosporosis, and a safe and effective vaccine suitable for cattle is required. dense granule protein 7 of n. caninum (ncgra7) is a secretory protein with high antigenicity in hosts. we demonstrated previously that ncgra7 entrapped in liposomes coated with mannotriose (m3-ncgra7) could induce a parasite-specific t-helper type 1 immune response and produce humor ... | 2013 | 23742998 |
| sag2a protein from toxoplasma gondii interacts with both innate and adaptive immune compartments of infected hosts. | toxoplasma gondii is an intracellular parasite that causes relevant clinical disease in humans and animals. several studies have been performed in order to understand the interactions between proteins of the parasite and host cells. sag2a is a 22 kda protein that is mainly found in the surface of tachyzoites. in the present work, our aim was to correlate the predicted three-dimensional structure of this protein with the immune system of infected hosts. | 2013 | 23735002 |
| gra 14, a novel dense granule protein from neospora caninum. | 2013 | 23722878 | |
| generation and immunity testing of a recombinant adenovirus expressing ncsrs2-ncgra7 fusion protein of bovine neospora caninum. | neospora caninum is the etiologic agent of bovine neosporosis, which affects the reproductive performance of cattle worldwide. the transmembrane protein, ncsrs2, and dense-granule protein, ncgra7, were identified as protective antigens based on their ability to induce significant protective immune responses in murine neosporosis models. in the current study, ncsrs2 and ncgra7 genes were spliced by overlap-extension pcr in a recombinant adenovirus termed ad5-ncsrs2-ncgra 7, expressing the ncsrs2- ... | 2013 | 23710096 |
| dose-dependent immunogenicity of a soluble neospora caninum tachyzoite-extract vaccine formulated with a soy lecithin/β-glucan adjuvant in cattle. | mice immunized with a soluble extract of neospora caninum tachyzoites (sncag) formulated with providean-avec, an aqueous soy-based adjuvant, are fully protected from n. caninum multiplication. here we evaluated the dose-dependent immunogenicity of this vaccine formulation in cattle. cattle (n=3 per group) were immunized with two applications (30 days apart) of formulations containing providean-avec and different payloads of sncag (100, 50 and 10 μg), that were five to fifty times lower than the ... | 2013 | 23692926 |
| comparison of use of vero cell line and suspension culture of murine macrophage to attenuation of virulence of neospora caninum. | in this study the tachyzoite yields of neospora caninum were compared in two cell lines: vero (african green monkey kidney) and suspension culture of murine macrophage (j774) cell lines. then, n. caninum were continuously passaged in these cell lines for 3 months and the effect of host cells on virulence of tachyzoites was assessed by broiler chicken embryonated eggs. inoculation was performed in the chorioallantoic (ca) liquid of the embryonated eggs with different dilutions (0.5 × 10(4), 1.0 × ... | 2013 | 23684321 |
| expression of neospora caninum ncsrs2 surface protein in pichia pastoris and its application for serodiagnosis of neospora infection. | neospora caninum is considerd a major cause of abortion in cattle worldwide. the antigenic domain of ncsrs2 in n. caninum is an important surface antigen present in the membrane of this parasite. in the present study, the pichia pastoris expression system proved to be a useful tool for the production of recombinant protein. the truncated ncsrs2 gene (by removal of the n-terminal hydrophobic sequence), was cloned in the vector ppiczalphab, and integrated on the genome of the methylotrophic yeast ... | 2013 | 23683365 |
| neospora caninum nc-6 argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams. | neospora caninum infection is a major cause of abortion in cattle. the objectives of this study were to genetically characterize the n. caninum nc-6 argentina isolate using a multilocus microsatellite analysis approach and to study its biological behavior by experimental inoculations into seronegative and seropositive pregnant cattle, evaluating the humoral and cellular immune response elicited and the occurrence of transplacental transmission and fetopathy. pregnant cows (65 days of gestation) ... | 2013 | 23666227 |
| parasiticidal activity of a novel synthetic peptide from the core α-helical region of nk-lysin. | nk-lysin is an anti-microbial peptide that plays a critical role in innate immunity against infectious pathogens through its selective membrane disruptive property. we previously expressed and purified a full-length chicken nk-lysin (cnkl) recombinant protein, and demonstrated its in vitro anti-parasitic activity against the apicomplexan protozoan, eimeria, the etiologic agent of avian coccidiosis. this study evaluated the in vitro and in vivo anti-parasitic properties of a synthetic peptide (cn ... | 2013 | 23664157 |
| bovine neosporosis: clinical and practical aspects. | neospora caninum is a protozoan parasite with a wide host range but with a preference for cattle and dogs. since the description of n. caninum as a new genus and species in 1988, bovine neosporosis has become a disease of international concern as it is among the main causes of abortion in cattle. at present there is no effective treatment or vaccine. this review focuses on the epidemiology of the disease and on prospects for its control in cattle. finally, based on the implications of clinical f ... | 2013 | 23659742 |
| neospora caninum: infection induces high lysosomal activity. | neospora caninum is a protozoan that causes abortion in cattle and neuromuscular lesions in dogs, making it an important target of veterinary medicine. lysosomes are cellular organelles responsible for important biological functions as cellular defense mechanisms. the aim of this work was to evaluate the lysosomal stability of rat gliocytes infected in vitro with n. caninum. rat glial cultures were infected at a ratio of 1:1 (cell/parasite). the enzymatic activity of acid phosphatase (orthophosp ... | 2013 | 23648665 |
| a novel dense granule protein, gra22, is involved in regulating parasite egress in toxoplasma gondii. | the intracellular protozoan parasite toxoplasma gondii is capable of invading any nucleated cell and replicates within a parasitophorous vacuole (pv). this microenvironment is modified by secretory proteins from organelles named rhoptries and dense granules. in this report, we identify a novel dense granule protein, which we refer to as gra22. gra22 has no significant homology to any other known proteins. gra22 possesses a signal peptide at the n-terminal end which is responsible for dense granu ... | 2013 | 23623919 |
| congenital transmission of neospora caninum in white-tailed deer (odocoileus virginianus). | neosporosis is an important cause of bovine abortion worldwide. many aspects of transmission of neospora caninum in nature are unknown. the white-tailed deer (odocoileus virginianus) is considered one of the most important wildlife reservoirs of n. caninum in the usa. during the hunting seasons of 2008, 2009, and 2010, brains of 155 white-tailed deer fetuses were bioassayed in mice for protozoal isolation. viable n. caninum (ncwtdmn1, ncwtdmn2) was isolated from the brains of two fetuses by bioa ... | 2013 | 23566408 |
| diagnosis and isolation of toxoplasma gondii in horses from brazilian slaughterhouses. | this study aimed to investigate anti-toxoplasma gondii antibodies and to isolate the parasite from the brains of horses processed at slaughterhouses in brazil. we collected brain and blood samples from 398 horses of various ages, from six brazilian states. serum samples were evaluated by indirect fluorescent antibody test (ifat cut-off titre ≥ 1:64), and brains were submitted to mouse bioassay. among the 398 horses, positivity for t. gondii was identified in 46 (11.6%) by ifat and in 14 (3.5%) b ... | 2013 | 23538498 |
| antibody kinetics in goats and conceptuses naturally infected with neospora caninum. | neospora caninum is a protozoan which can cause abortions in caprines. however, information regarding the humoral immune response and the occurrence of reproductive disorders is scarce. this is the first study in which the kinetics of antibodies is studied in pregnant goats naturally infected by n. caninum, as well as their respective conceptuses. the subclasses of igg (igg1 and igg2) were also evaluated in pregnant goats. reproductive problems related to neosporosis (abortion and stillbirth) oc ... | 2013 | 23537945 |
| efficacy of decoquinate against sarcocystis neurona in cell cultures. | decoquinate is a quinolone anticoccidial approved for use in the prevention of intestinal coccidiosis in farm animals. this compound has good activity against toxoplasma gondii and neospora caninum in cell cultures. the drug acts on the parasites' mitochondria. the activity of decoquinate against developing merozoites of 2 isolates of sarcocystis neurona was examined in cell culture. merozoite production at 10 days was completely inhibited when decoquinate was used at 20 or 240 nm. the ic50 of d ... | 2013 | 23523012 |
| display of neospora caninum surface protein related sequence 2 on rous sarcoma virus-derived gag protein virus-like particles. | virus-like particles (vlps) displaying antigen have been increasingly recognized as a potential vaccine in the livestock industry. in this study, neospora caninum surface protein related sequence (ncsrs)2 was displayed on the surface of rous sarcoma virus group-antigen protein (rsv-gag) vlps. two types of bombyx mori nucleopolyhedrovirus (bmnpv) bacmids, encoding rsv-gag and ncsrs2 genes, were co-injected into silkworm larvae to produce vlps-ncsrs2. at 7 days post-injection, vlps-ncsrs2 were col ... | 2013 | 23511652 |
| mice congenitally infected with low-to-moderate virulence neospora caninum isolates exhibited clinical reactivation during the mating period without transmission to the next generation. | endogenous transplacental transmission (entt) is the major transmission route of neospora caninum in cattle. thus, the development of a standardised experimental model of entt is needed for more appropriate testing of parasite biology and control strategies. a recent study reported up to 40-50% entt rates in chronically infected dams with either high or low-to-moderate virulence isolates, although low fertility rates were observed in dams inoculated with the high virulence isolate. therefore, lo ... | 2013 | 23501806 |
| first report of seroprevalence of toxoplasma gondii and neospora caninum in dairy sheep from humid pampa, argentina. | the aim of this study was to describe the occurrence of antibodies to toxoplasma gondii and neospora caninum in dairy sheep from the humid pampa region, argentina. blood samples from 704 dairy sheep belonging to six flocks were collected. using a cut off titer of 1:50, an indirect fluorescence antibody test was used. antibodies to t. gondii or n. caninum were detected in 17.3 % (n = 122) and 3 % (n = 21), respectively. all the flocks had at least one seropositive animal to t. gondii but two of t ... | 2013 | 23494577 |
| development of a disperse dye immunoassay technique for detection of antibodies against neospora caninum in cattle. | in this study a disperse dye immunoassay method was standardized and evaluated for detection of antibodies against neospora caninum in cattle. sera from 150 cattle with a recent history of abortion were collected and tested by commercial elisa kit and a standardized in-house dye immunoassay system. the positivity rate for the sera used in this study was 34.6% for the disperse dye immunoassay (ddia) compared to 32% obtained by elisa kit. this study showed no significant difference between ddia an ... | 2013 | 23467930 |
| risk factors associated with seroprevalence of neospora caninum in dogs from urban and rural areas of milk and coffee production in minas gerais state, brazil. | this study was conducted to determine the seroprevalence of anti-neospora caninum antibodies and to investigate the risk factors related to seroprevalence in dogs from urban and rural areas with distinct economic activities (milk and coffee production) in minas gerais state, brazil. for this purpose, blood samples from 703 dogs were collected and questionnaires addressing epidemiological aspects were completed by dog-owners. the sera were analysed for anti-n. caninum antibodies by indirect fluor ... | 2013 | 23419686 |
| development of an immunochromatographic assay based on dense granule protein 7 for serological detection of toxoplasma gondii infection. | dense granule antigen proteins derived from toxoplasma gondii (tggras) are potential antigens for the development of diagnostic tools. tggra7 and tggra14 were detected in the peritoneal fluid of t. gondii-infected mice, suggesting that tggras may be highly antigenic proteins. here, tggra7 and tggra14 were evaluated as candidates for the development of a marker for a rapid diagnostic test. the specificity and sensitivity of purified recombinant proteins of tggra7 and tggra14 were compared in an i ... | 2013 | 23408523 |
| effects of neospora caninum infection on brain microvascular endothelial cells bioenergetics. | the brain is the most commonly affected organ during neospora caninum infection but the mechanisms utilized by this protozoan parasite for traversal of the blood-brain barrier (bbb) are not yet understood. herein, we investigate the cellular pathogenicity of n. caninum infection on bioenergetics of human brain microvascular endothelial cells (hbmecs), a fundamental component of the bbb. | 2013 | 23351811 |
| specific antibody responses against neospora caninum recombinant rncgra7, rncsag4, rncbsr4 and rncsrs9 proteins are correlated with virulence in mice. | the intraspecific diversity of neospora caninum is a determinant for in vivo parasite virulence and in vitro parasite behaviour. the relationship between isolate virulence and specific antibody responses against key parasite proteins has not been well characterized. the response kinetics and the differences in specific anti-rncgra7, -rncsag4, -rncbsr4 and -rncsrs9 antibody levels were analysed by recombinant protein-based elisa in groups of mice inoculated with 10 different n. caninum isolates t ... | 2013 | 23343779 |
| neospora caninum causes severe economic losses in cattle in the humid pampa region of argentina. | this work estimates the economic losses due to neospora abortions in the humid pampa region of argentina.the total dairy and beef cattle population at risk of abortion is 1,771,326 and 9,726,684 head, respectively. in dairy cattle, there was an 8 % risk of experiencing abortion due to a variety of causes, but 16.5 % of them were due to neospora caninum. the economic losses were estimated at us$1,415 (1,400-1,431) per abortion, which equates to a total loss of us$33,097,221 (15,622,600-119,349,69 ... | 2013 | 23325013 |
| development of two murine antibodies against neospora caninum using phage display technology and application on the detection of n. caninum. | neosporosis, caused by an intracellular parasite, neospora caninum, is an infectious disease primarily of cattle and dogs. it occurs worldwide and causes huge damages to dairy farms. in this study, we immunized mice with recombinant surface-associated protein 1 of n. caninum (rncsag1) and developed two novel monoclonal antibodies, a10 and h3, against ncsag1 using phage-display technology. both clones bound to purified rncsag1 and the half maximal inhibitory concentrations of a10 and h3 are 50 an ... | 2013 | 23308179 |
| proteins mediating the neospora caninum-host cell interaction as targets for vaccination. | neospora caninum is an apicomplexan parasite that is capable of infecting, a wide range of tissues. the fact that neospora represents an important abortion-causing parasite in cattle has transformed neosporosis research from an earlier, rather esoteric field, to a significant research topic, and considerable investments have been made in the last years to develop an efficacious vaccine or other means of intervention that would prevent infection and abortion due to n. caninum infection in cattle. ... | 2013 | 23276967 |
| differential effects on survival, humoral immune responses and brain lesions in inbred balb/c, cba/ca, and c57bl/6 mice experimentally infected with neospora caninum tachyzoites. | c57bl/6, balb/c, and cba/ca mouse strains with different mhc-i haplotypes were compared with respect to susceptibility to neospora caninum infection. groups of 5 mice received 1 × 10(6), 5 × 10(6), or 25 × 10(6) tachyzoites of the nc-liverpool isolate by intraperitoneal injection and were observed for disease symptoms. humoral responses, splenocyte interferon-γ (ifn-γ) production, cerebral parasite loads, and histopathology were evaluated at human end points or the latest at 34 days postinfectio ... | 2013 | 27335863 |
| use of a th1 stimulator adjuvant for vaccination against neospora caninum infection in the pregnant mouse model. | vertical transmission from an infected cow to its fetus accounts for the vast majority of new neospora caninum infections in cattle. a vaccine composed of a chimeric antigen named recncmic3-1-r, based on predicted immunogenic domains of the two microneme proteins ncmic1 and ncmic3, the rhoptry protein ncrop2, and emulsified in saponin adjuvants, significantly reduced the cerebral infection in non-pregnant balb/c mice. protection was associated with a mixed th1/th2-type cytokine response. however ... | 2013 | 25437035 |
| seroprevalence of neospora caninum and associated risk factors in intensive or semi-intensively managed dairy and breeding cattle of ethiopia. | a cross-sectional study of neospora caninum infection was conducted in major milksheds of ethiopia. cattle (n=2334) from 273 farms were bled and the sera screened for antibodies against n. caninum using a commercial elisa kit. herd and individual animal level data were collected from farm records and a semi-structured questionnaire format. the overall animal level seroprevalence was 13.3%, while the prevalence at farm level was 39.6%. in urban and peri-urban smallholder dairy farms, the seroprev ... | 2013 | 23261087 |
| what is the global economic impact of neospora caninum in cattle - the billion dollar question. | neospora caninum is regarded as one of the most important infectious causes of abortions in cattle worldwide, yet the global economic impact of the infection has not been established. a systematic review of the economic impact of n. caninum infections/abortions was conducted, searching pubmed with the terms 'cattle' and 'neospora'. this yielded 769 publications and the abstracts were screened for economically relevant information (e.g. abortion prevalence and risk, serological prevalence). furth ... | 2013 | 23246675 |
| tissue distribution of neospora caninum in experimentally infected cattle. | histopathology and quantitative pcr (qpcr) were used to determine the tissue distribution of neospora caninum in calves at 80 days postinfection. our findings revealed that the most appropriate brain areas for researching n. caninum pathogenesis were the amygdala and hippocampus for qpcr and the corpus striatum and diencephalon for histopathology. | 2013 | 23239805 |
| comparative study of protective activities of neospora caninum bradyzoite antigens, ncbag1, ncbsr4, ncmag1, and ncsag4, in a mouse model of acute parasitic infection. | neospora caninum is an obligate intracellular protozoan parasite that causes severe neuromuscular diseases, repeated abortion, stillbirth, and congenital infection in livestock and companion animals. the development of an effective vaccine against neosporosis in cattle is an important issue due to the significant worldwide economic impact of this disease. we evaluated the immunogenicity of four bradyzoite antigens, ncbag1 (first described in this study), ncbsr4, ncmag1, and ncsag4, using an acut ... | 2013 | 23229317 |
| plasma concentrations of pregnancy-associated glycoproteins measured using anti-bovine pag-2 antibodies on day 120 of gestation predict abortion in dairy cows naturally infected with neospora caninum. | the present study sought to determine: (i) the effects of neospora caninum infection and twin pregnancy on plasma pregnancy-associated glycoprotein-2 (pag-2) concentrations throughout pregnancy and (ii) whether plasma pag-2 concentrations could predict abortion in n. caninum-infected cows. the study was performed on a commercial holstein-friesian dairy herd in northeastern spain and the final data included those recorded in 53 non-aborting and 19 aborting animals. blood samples were collected im ... | 2013 | 23228018 |
| factors associated with seroprevalence of neospora caninum in dairy cattle in southeastern brazil. | neosporosis is an infectious disease caused by neospora caninum, an obligate intracellular cyst-forming protozoan considered a major cause of miscarriage in dairy cattle in many parts of the world. this cross-sectional study evaluated the relationship between reproductive abnormalities and seropositivity to n. caninum in 1,204 dairy cows from 40 farms located in the state of minas gerais, southeastern brazil. producers were interviewed, and blood samples were collected to perform indirect immuno ... | 2013 | 23212838 |
| seroprevalence for neospora caninum in goats of minas gerais state, brazil. | this study was aimed at determining the prevalence of anti-neospora caninum antibodies in serum samples from 667 goats, collected from 90 herds in 90 municipalities in eight of the twelve mesoregions of minas gerais state. the presence of anti-n. caninum antibodies was determined by ifat with a cut-off value of 1:50. the true herd-level seroprevalence in the entire study area was 75.2% (ci 95%: 59.5-90.9%). the true individual-level seroprevalence in the entire study area was 10.7% (ci 95%: 8.3- ... | 2013 | 23200513 |
| unravelling the neospora caninum secretome through the secreted fraction (esa) and quantification of the discharged tachyzoite using high-resolution mass spectrometry-based proteomics. | the apicomplexan parasite neospora caninum causes neosporosis, a disease that leads to abortion or stillbirth in cattle, generating an economic impact on the dairy and beef cattle trade. as an obligatory intracellular parasite, n. caninum needs to invade the host cell in an active manner to survive. the increase in parasite cytosolic ca2+ upon contact with the host cell mediates critical events, including the exocytosis of phylum-specific secretory organelles and the activation of the parasite i ... | 2013 | 24267406 |
| neospora caninum: evaluation of vertical transmission in slaughtered dairy cows (bos taurus). | neospora caninum is a worldwide parasite recognized as one of the main parasites responsible for abortion in cattle. the objective of this study was to evaluate vertical transmission of n. caninum in dairy cows (bos taurus) that were slaughtered at an abattoir in the state of santa catarina, southern brazil. blood samples (with and without edta) from 60 pregnant dairy cows and blood and tissue samples (brain, lung, heart and liver) from their fetuses were collected and used for pcr and serologic ... | 2013 | 24252950 |
| hepatic neosporosis in a dog treated for pemphigus foliaceus. | a 4-year-old, female, spayed border collie dog was presented for progressive lethargy, inappetence, and weakness of 4 days duration. the animal had been diagnosed with pemphigus foliaceus 3 months prior and was receiving combination immunosuppressive therapy. serum biochemistry revealed severely elevated liver enzymes and bilirubin, and humane euthanasia was elected. gross postmortem examination revealed a diffusely pale tan to slightly yellow, enlarged, markedly friable liver with an enhanced r ... | 2013 | 24202993 |
| prevalence of antibodies against toxoplasma gondii and neospora caninum in dogs. | toxoplasmosis and neosporosis are parasitic diseases of global importance. the present study had the objective to determine the influence of age, sex and breed in the prevalence of antibodies against both diseases in dogs from brotas city, são paulo state, brazil. blood samples of 342 dogs were collected, and the age, sex and breed of each animal were recorded. the serological diagnosis for toxoplasmosis and neosporosis were performed using the immunofluorescent antibody test (ifat). the fischer ... | 2013 | 24688530 |
| neospora caninum exposure in overlapping populations of coyotes (canis latrans) and feral swine (sus scrofa). | limited information exists on neospora caninum transmission dynamics in wildlife. this coccidian parasite, whose presence can lead to substantial economic losses in cattle operations, requires a canid definitive host for reproduction. we examined exposure in a definitive host, coyotes (canis latrans), and in overlapping populations of feral swine (sus scrofa) to determine if spatial proximity between a definitive and incidental host influences the likelihood of parasite exposure. eighteen percen ... | 2013 | 24502735 |
| toxoplasma gondii and neospora caninum seroprevalence in dairy sheep and goats mixed stock farming. | toxoplasma and neospora infections are important causes of abortions and economic losses in animal production. mixed stock farming of sheep and goats is a common practice in mediterranean countries and could serve as a suitable model for the evaluation of differences between the two animal species regarding parasitic infections. in order to investigate the seroprevalence of t. gondii and n. caninum among flocks of small ruminants in greece and to evaluate any prevalence difference between sheep ... | 2013 | 24103737 |
| study on outbreak of neospora caninum-associated abortion in dairy cows in tabriz (northwest iran) by serological, molecular and histopathologic methods. | to determine neospora caninum (n. caninum) as a cause of bovine abortion in dairy cows by elisa, pcr and pathological methods in tabriz, northwest of iran. | 2013 | 24144024 |