Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| rotavirus genotypes in belarus, 2008-2012. | this study describes group a rotavirus (rva) genotype prevalence in belarus from 2008 to 2012. in 2008, data from 3 sites in belarus (brest, mogilev, minsk) indicated that g4p[8] was the predominant genotype. data from minsk (2008-2012) showed that g4p[8] was the predominant rva genotype in all years except in 2011 when g3p[8] was most frequently detected. other rva genotypes common in europe (g1p[8], g2p[4]) were detected each year of the study. this study reveals the dominance of genotype g4p[ ... | 2014 | 25218086 |
| molecular surveillance of rotavirus infection in bangui, central african republic, october 2011-september 2013. | the world health organization (who) recommends the introduction of rotavirus vaccine in the immunization program of all countries. in the central african republic (car), sentinel surveillance for rotavirus gastroenteritis was established in 2011 by the ministry of health, with the support of the surveillance en afrique centrale project (survac). the purpose of this study was to assess the burden of rotavirus gastroenteritis and to identify rotavirus strains circulating in car before the introduc ... | 2014 | 25193563 |
| metagenomic identification of novel enteric viruses in urban wild rats and genome characterization of a group a rotavirus. | rats are known as reservoirs and vectors for several zoonotic pathogens. however, information on the viruses shed by urban wild rats that could pose a zoonotic risk to human health is scare. here, intestinal contents from 20 wild norway rats (rattus norvegicus) collected in the city of berlin, germany, were subjected to metagenomic analysis of viral nucleic acids. the determined faecal viromes of rats consisted of a variety of known and unknown viruses, and were highly variable among the individ ... | 2014 | 25121550 |
| whole genomic analysis of human g12p[6] and g12p[8] rotavirus strains that have emerged in kenya: identification of porcine-like nsp4 genes. | g12 rotaviruses are globally emerging rotavirus strains causing severe childhood diarrhea. however, the whole genomes of only a few g12 strains have been fully sequenced and analyzed, of which only one g12p[4] and one g12p[6] are from africa. in this study, we sequenced and characterized the complete genomes of three g12 strains (rva/human-tc/ken/kdh633/2010/g12p[6], rva/human-tc/ken/kdh651/2010/g12p[8], and rva/human-tc/ken/kdh684/2010/g12p[6]) identified in three stool specimens from children ... | 2014 | 25111611 |
| molecular epidemiology of rotavirus in children and animals and characterization of an unusual g10p[15] strain associated with bovine diarrhea in south india. | rotaviruses are enteric pathogens causing acute, watery, dehydrating diarrhea in various host species, including birds and mammals. this study collected data on the disease burden and strain prevalence of group a rotavirus in animals and humans in vellore and investigated interspecies transmission by comparison of circulating genotypes. stool samples from children aged less than 5 years, admitted to the hospital between january 2003 and may 2006 for diarrhea and diarrheal samples from animals th ... | 2014 | 25091687 |
| characterisation of rotavirus strains identified in adolescents and adults with acute gastroenteritis highlights circulation of non-typeable strains: 2008-2012. | group-a rotavirus (rv) is the main causative agent of acute gastroenteritis in children < 5 years of age. its role as a pathogen in adults needs to be monitored. the aim of this study was to characterise the group-a rv strains that cause infections of acute gastroenteritis in adolescents and adults and determine the temporal variations in the circulating strains during 2008-2012 in continuation of an earlier study conducted in 2004-2007, in pune, india. | 2014 | 25091684 |
| group a rotavirus gastroenteritis in older children and adults at a hospital in southern india. | there is limited data on the spectrum and prevalence of rotavirus genotypes in older children and adults in asia. this pilot study conducted between november 2012 and april 2013 tested for rotavirus in older children (>12 years of age), and adults with gastroenteritis from southern india. stool samples from patients who were hospitalized or attended out-patient units with diarrhea were screened for rotavirus using premier™ rotaclone(®). confirmatory testing was by another antigen detection sandw ... | 2014 | 25091677 |
| acute gastroenteritis and enteric viruses in hospitalised children in southern brazil: aetiology, seasonality and clinical outcomes. | viral acute gastroenteritis (ag) is a significant cause of hospitalisation in children younger than five years. group a rotavirus (rva) is responsible for 30% of these cases. following the introduction of rva immunisation in brazil in 2006, a decreased circulation of this virus has been observed. however, ag remains an important cause of hospitalisation of paediatric patients and only limited data are available regarding the role of other enteric viruses in these cases. we conducted a prospectiv ... | 2014 | 25075782 |
| human group a rotavirus infections in children in denmark: detection of reassortant g9 strains and zoonotic p[14] strains. | one of the leading causes of severe childhood gastroenteritis are group a rotaviruses, and they have been found to be associated with ∼40% of the annual gastroenteritis-associated hospitalizations in young danish children <5years of age (fischer et al., 2011). in this study, we investigated the diversity of rotavirus strains circulating among young children <5years of age, presenting with gastroenteritis disease either at the general practitioner or in the hospital, during the period 2009-2013. ... | 2014 | 25038295 |
| gastroenteric virus dissemination and influence of rainfall events in urban beaches in brazil. | this study was conducted to assess rotavirus a (rv-a), genogroup ii (gii) norovirus (nov), and human adenovirus (hadv) dissemination in recreational water in an urban beach located in the city of rio de janeiro and their persistence during rainfall events. | 2014 | 24980661 |
| rotavirus surveillance in kisangani, the democratic republic of the congo, reveals a high number of unusual genotypes and gene segments of animal origin in non-vaccinated symptomatic children. | group a rotavirus (rva) infections form a major public health problem, especially in low-income countries like the democratic republic of the congo (cod). however, limited data on rva diversity is available from sub-saharan africa in general and the cod in particular. therefore, the first aim of this study was to determine the genetic diversity of 99 rvas detected during 2007-2010 in kisangani, cod. the predominant g-type was g1 (39%) and the most predominant p-type was p[6] (53%). a total of ei ... | 2014 | 24968018 |
| complete genome analysis of a rare group a rotavirus, g11p[25], isolated from a child in mumbai, india, reveals interspecies transmission and reassortment with human rotavirus strains. | hospital-based rotavirus surveillance was carried out in mumbai during 2005-2009. an isolate (b08299) with a rare genotype combination (g11p[25]) was detected. the present study was undertaken to characterize the complete genome of the isolate. b08299 exhibited a g11-p[25]-i12-r1-c1-m1-a1-n1-t1-e1-h1 genotype constellation. phylogenetic analysis of the 11 gene segments of b08299 revealed that the vp2 and nsp5 genes of b08299 had a human origin, while the vp6 gene represented an i12 genotype of o ... | 2014 | 24951672 |
| [whole genome analysis of human group a rotavirus g9p[8] strains in hebei lulong region, 2009-2011]. | abstract:this study aims to investigate the genetic characteristics of group a rotavirus (garv) g9p[8] strains from infantile diarrhea samples in hebei lulong region from 2009 to 2011. we randomly selected five garv g9p[8] strains in hebei lulong region from 2009 to 2011, amplified the 11 gene fragments of garvs by rt-pcr, and analyz their full-genome sequences by homology and phylogenetic analysis with dnastar and mega. the nucleotide homology between strains ll11131077 and ll11131083 in 2011 w ... | 2014 | 24923163 |
| surveillance of group a rotavirus in buenos aires 2008-2011, long lasting circulation of g2p[4] strains possibly linked to massive monovalent vaccination in the region. | group a rotaviruses (rva) are the most frequent single etiological agents of severe diarrhea in infants. since 2006 rva vaccines have been introduced in national schedules of middle and high income countries with substantial declines in rotavirus associated disease burden. however, surveillance must be maintained to, eventually, detect emerging types or variants selected by the new pressure imposed by vaccination. | 2014 | 24875137 |
| comparison of pathogenicities and nucleotide changes between porcine and bovine reassortant rotavirus strains possessing the same genotype constellation in piglets and calves. | although reassortment is one of the most important characteristics of group a rotavirus (rva) evolution, the host range restriction and/or virulence of reassortant rvas remain largely unknown. the porcine 174-1 strain isolated from a diarrheic piglet was identified as a reassortant strain, harboring the same genotype constellation as the previously characterized bovine strain kj56-1. owing to its same genotype constellation, the pathogenicity of the porcine strain 174-1 in piglets and calves was ... | 2014 | 24861840 |
| gastroenteritis outbreaks caused by a ds-1-like g1p[8] rotavirus strain, japan, 2012-2013. | rotavirus a (rva) genotype g1p[8], a hallmark of the wa-like strain, typically contains only genotype 1 genes. however, an unusual rva g1p[8] with genotype 2 genes was recently detected in japan. we determined the complete genomic constellation of this rva. our findings suggest that mixed rvas may be more competitive than once thought. | 2014 | 24856174 |
| molecular detection of human noroviruses in influent and effluent samples from two biological sewage treatment plants in the region of monastir, tunisia. | noroviruses (novs) are responsible for numerous cases of waterborne and foodborne gastroenteritis every year. they are released in the sewage and their detection in this environment can reflect the epidemiology of the viral strains circulating in the community. a three-year (2007-2010) survey was conducted in order to evaluate the presence of human novs using rt-pcr in 518 sewage samples collected at the entrance and exit of two biological sewage treatment plants located in monastir region, tuni ... | 2014 | 24818568 |
| occurrence and characterization of rotavirus a in broilers, layers, and broiler breeders from brazilian poultry farms. | rotaviruses are a major cause of diarrhea in humans and animals, including several mammalian and avian species. using different pcr protocols, we report the occurrence of rotavirus a in 21 (53.84%; 21/39) from 39 fecal pool samples of broilers, layers, and broiler breeders from brazilian avian farms. we typed the g5, g8, g11, g19, and p[31] genotypes. | 2014 | 24758129 |
| distinct evolutionary origins of g12p[8] and g12p[9] group a rotavirus strains circulating in brazil. | g12 group a rotavirus (rva) are currently recognized as a globally emerging genotype and have been described in combination with several p-types. in brazil, g12 rva strains have been described in the southern (2003) and northern (2008-2010) regions, in combination with the p[9] and p[6] genotype, respectively. to date, few complete genomes of g12 rva strains have been described (none from brazilian strains), considering g12p[9] genotype just one strain, rva/human-tc/tha/t152/1998/g12p[9], has th ... | 2014 | 24747965 |
| [prognosis of rotavirus infection in children]. | the aim of the research was to develop the method for predicting the course of rotavirus gastroenteritis in children.under the supervision were 3607 children aged from 9 days to 5 years with the diagnosis of "acute gastroenteritis" and "acute gastroenterocolitis". the diagnosis of rotavirus infection was on the basis of a set of clinical, epidemiological data and the results of para-clinical and bacteriological studies and data of detection of rotavirus antigen strain. genotyping of rotavirus gr ... | 2014 | 24743127 |
| the first caprine rotavirus detected in argentina displays genomic features resembling virus strains infecting members of the bovidae and camelidae. | rotavirus group a (rva) is a major cause of diarrhea in humans and young animals including small ruminants. the purpose of this study was to identify rva in dairy goat kids, and to characterize the complete genomic constellation and genetic relatedness with other rva strains. four out of twenty fecal samples from diarrheic and non-diarrheic goat kids were positive for rva by elisa. a representative sample was selected for further genome analyses. the rva strain rva/goat-wt/arg/0040/2011/g8p[1] d ... | 2014 | 24742949 |
| identification of novel bovine group a rotavirus g15p[14] strain from epizootic diarrhea of adult cows by de novo sequencing using a next-generation sequencer. | there are few reports describing diarrhea of adult cattle caused by group a rotaviruses. here, we report the identification of a novel bovine group a rotavirus from diarrhea of adult cows. a group a rotavirus was detected from an epizootic outbreak of diarrhea in adult cows with a decrease in milk production in japan in 2013. the comprehensive genomic analyses from fecal samples by viral metagenomics using a next-generation sequencer revealed that it had an unreported genotype combination g15p[1 ... | 2014 | 24725447 |
| evaluation of the rapid test for human rotavirus a in thai children with acute gastroenteritis. | rapid tests are widely used to detect rotavirus a; however, pediatricians are concerned whether the rapid test can still accurately detect the virus. therefore, this study evaluated the performance of the rotavirus rapid test by comparing it to the one-step rt-pcr method. | 2014 | 24697131 |
| virucidal effect of commercially available disinfectants on equine group a rotavirus. | although many disinfectants are commercially available in the veterinary field, information on the virucidal effects of disinfectants against equine group a rotavirus (rva) is limited. we evaluated the performance of commercially available disinfectants against equine rva. chlorine- and iodine-based disinfectants showed virucidal effects, but these were reduced by the presence of organic matter. glutaraldehyde had a virucidal effect regardless of the presence of organic matter, but the effect wa ... | 2014 | 24681569 |
| sequence and structural analyses of nsp4 proteins from human group a rotavirus strains detected in tunisia. | the nsp4 protein of group a rotavirus (rva) has been recognized as a viral enterotoxin and plays important roles in viral pathogenesis and morphogenesis. domains involved in structural and functional interactions have been proposed mainly based on the simian sa11 strain. | 2014 | 24679587 |
| full-genome sequence of a rare human g3p[9] rotavirus strain. | this is a report of the complete genomic sequence of a rare rotavirus group a g3-p[9]-i2-r2-c2-m2-a3-n2-t1-e2-h3 strain designated rva/human-wt/usa/12us1134/2012/g3p[9]. | 2014 | 24675848 |
| predominance of rotavirus g8 genotype in hospitalized children with acute gastroenteritis in yasuj, iran. | rotavirus infection is the most common cause of gastroenteritis among children worldwide. in this study, we were evaluated the occurrence of rotavirus infection and genotypes causing gastroenteritis among children under 5 years in yasuj, iran. | 2014 | 24668711 |
| diarrhoea-related hospitalizations in children before and after implementation of monovalent rotavirus vaccination in mexico. | to assess, by socioeconomic setting, the effect of nationwide vaccination against species a rotavirus (rva) on childhood diarrhoea-related hospitalizations in mexico. | 2014 | 24623905 |
| surveillance of pathogens causing gastroenteritis and characterization of norovirus and sapovirus strains in shenzhen, china, during 2011. | viral gastroenteritis is one of the most common diseases in humans, and it is primarily caused by rotaviruses (rvs), astroviruses (astvs), adenoviruses (advs), noroviruses (novs), and sapoviruses (savs). in this study, we determined the distribution of viral gastroenteritis and human calicivirus (hucvs) in acute gastroenteritis patients in shenzhen, china, during 2011. real-time rt-pcr was used to detect norovirus (nov), group a rotavirus (rv), adenovirus (adv), and astrovirus (astv). from a tot ... | 2014 | 24610551 |
| complete genomic sequence analyses of the first group a giraffe rotavirus reveals close evolutionary relationship with rotaviruses infecting other members of the artiodactyla. | group a rotaviruses (rva) have been established as significant contributory agents of acute gastroenteritis in young children and many animal species. in 2008, we described the first rva strain detected in a giraffe calf (rva/giraffe-wt/irl/girrv/2008/g10p[11]), presenting with acute diarrhoea. molecular characterisation of the vp7 and vp4 genes revealed the bovine-like genotypes g10 and p[11], respectively. to further investigate the origin of this giraffe rva strain, the 9 remaining gene segme ... | 2014 | 24582453 |
| diversity of rotavirus strain circulated in gwangju, republic of korea. | the introduction of new rotavirus vaccines into the public sphere makes it necessary to maintain constant surveillance and to heighten public awareness of the appearance of new rotavirus strains. we describe the molecular epidemiology of circulating rotavirus strains after vaccine introduction. | 2014 | 25562046 |
| rotavirus infection in children and adults with acute gastroenteritis in thailand. | and young children, but rotavirus gastroenteritis in adults is uncommon. in this study, 260 stool samples collected in thailand from january 2006 to february 2007 from patients, of all ages with acute gastroenteritis, were tested for group a rotavirus and compared with rotavirus infections in children and adults. rota- virus was detected in 42% of the patients' samples, but children (< 18 years old) have a significantly higher prevalence (57%) of rotavirus infection than adults (≥ 18 years old) ... | 2014 | 25507599 |
| full genome sequence of a reassortant human g9p[4] rotavirus strain. | this is a report of the complete genomic sequence of a reassortant rotavirus group a g9-p[4]-i2-r2-c2-m2-a2-n2-t2-e6-h2 strain designated rva/human-wt/usa/ lb1562/2010/g9p[4]. | 2014 | 25502675 |
| rotavirus strains in neglected animal species including lambs, goats and camelids. | surveillance of rotavirus infections and circulating strains in small ruminants (i.e. lambs, goats and camelids) has been a neglected research area in the past. however, recent years that have seen an intensification of surveillance in humans and livestock animals, where vaccines to reduce disease burden caused by rotavirus a (rva) are available, led to the efforts to better understand the epidemiology, ecology and evolution of rva strains in other hosts, including lambs, goats and camelids. the ... | 2014 | 25674588 |
| epidemiological and clinical studies of rotavirus-induced diarrhea in china from 1994-2013. | group a rotavirus infections cause diarrhea in infants and primarily occur in winter. these viruses are characterized by the viral structural proteins vp7 (g subtypes) and vp4 (p subtypes). current treatments employ vaccines combined with symptomatic treatments. through a review of published papers from 2003-2013, which included articles, theses, dissertations and academic conference proceedings in pubmed and cnki (china national knowledge infrastructure), we examined the epidemiological and cli ... | 2014 | 25668671 |
| molecular characterization of human g8p[4] rotavirus strains in italy: proposal of a more complete subclassification of the g8 genotype in three major lineages. | in 2011, two children with acute rotavirus gastroenteritis were hospitalized in sardinia, italy. two rva strains with g8p[4] genotype were detected in their stools, and were named ss56/2011 and ss65/2011. the aim of the study was to characterize these two rare strains, collected within a national rva gastroenteritis surveillance program. eight of the 11 rva genes were sequenced and phylogenetic analysis performed. vp7 amino acid sequence was also analyzed. sequencing of genes encoding the vp4, v ... | 2014 | 24252348 |
| detection of rotavirus in children with acute gastroenteritis in porto velho, rondonia, brazil. | rotavirus group a (rva) infection was ascertained in 591 fecal samples from children 0 to 6 years old with acute gastroenteritis. the vaccination status was also verified in all 591 subjects, with 302 (51.0 % ± 4.0 %) participants fully vaccinated against rotavirus. forty-two of the vaccinated children (13.9 % ± 3.9 %) tested positive for rva infection. of the 289 unvaccinated children (49.0 % ± 4.0 %), 61 (21.1 % ± 4.7 %) had stools positive for rva. this study suggests that the proportion of a ... | 2014 | 24158345 |
| epidemiological and genetic analysis of human group c rotaviruses isolated from outbreaks of acute gastroenteritis in yokohama, japan, between 2006 and 2012. | group c rotavirus (gcrv) infection has been described in several parts of the world, predominantly as sporadic cases of acute gastroenteritis. little is known about the yearly changes in the gcrv strains from diarrheal outbreaks. stool samples collected from outbreaks of acute gastroenteritis in yokohama, japan, between 2006 and 2012 that were negative for norovirus, sapovirus, and group a rotavirus, were screened for gcrv using a reverse passive hemagglutination method. the gcrv strains were ch ... | 2014 | 24154950 |
| diversity of rotavirus strains circulating in northern brazil after introduction of a rotavirus vaccine: high prevalence of g3p[6] genotype. | rotavirus a (rva) is the most common cause of severe acute gastroenteritis in infants and young children worldwide, causing 453,000 deaths annually. in brazil, the most frequent genotype identified was g1 during almost three decades in the pre-vaccination period; however, after anti-rotavirus vaccine introduction, there was a predominance of g2 genotype. the aim of this study was to determine the g and p genotypes of rotaviruses isolated from children under 5 years of age with acute gastroenteri ... | 2014 | 24136444 |
| detection and molecular characterization of diarrhea causing viruses in single and mixed infections in children: a comparative study between bangladesh and turkey. | the incidence and mortality caused by diarrhea differ among countries. the prevalence of different enteric viruses, their molecular characteristics, and infections with multiple viruses might affect the disease incidence and mortality caused by diarrhea. the objective of this study was to determine the distribution and molecular characteristics of enteric viruses in children with diarrhea in turkey and bangladesh. a total of 288 stool samples that were negative for group a rotavirus were collect ... | 2014 | 24105741 |
| rotavirus in adults, brazil, 2004-2011: g2p[4] dominance and potential impact on vaccination. | the aim of this study was to monitor rotavirus (rv) infections in adults >18 years with acute gastroenteritis during 2004-2011 national brazilian rv surveillance. in addition, to characterize the rv group a (rva) strains in order to gain insight into the supposed vaccine selective pressure imposed to brazilian children population. | 2014 | 24076114 |
| genetic diversity of rotavirus strains circulating in environmental water and bivalve shellfish in thailand. | rotavirus is a common cause of acute diarrhea in young children worldwide. this study investigated the prevalence and molecular characterization of rotavirus in environmental water and oyster samples in thailand. a total of 114 water samples and 110 oyster samples were collected and tested for group a rotavirus using rt-nested pcr. rotavirus genotype was identified by phylogenetic analysis of the vp7 genetic sequences. group a rotavirus was detected in 21 water samples (18.4%) and six oyster sam ... | 2014 | 24469269 |
| characterization of the nsp4 gene of group a human rotavirus g1p[8] strains circulating in sapporo, japan from 1987 to 2000. | the genetic diversity of the nsp4 gene of rotavirus g1p[8] strains obtained in sapporo was analyzed, japan from 1987 to 2000. sixty-four strains, which were distributed across the whole study period, were included. all g1p[8] nsp4 genes detected in this study belonged to genotype e1, which divided into at least three lineages. the sapporo rotavirus g1p[8] isolates were found in each lineage. the mean estimated substitution rate was 1.40 × 10(-3) nucleotide substitutions per site per year, which ... | 2014 | 24026858 |
| rotavirus inhibits ifn-induced stat nuclear translocation by a mechanism that acts after stat binding to importin-α. | the importance of innate immunity to rotaviruses is exemplified by the range of strategies evolved by rotaviruses to interfere with the ifn response. we showed previously that rotaviruses block gene expression induced by type i and ii ifns, through a mechanism allowing activation of signal transducer and activator of transcription (stat) 1 and stat2 but preventing their nuclear accumulation. this normally occurs through activated stat1/2 dimerization, enabling an interaction with importin α5 tha ... | 2014 | 24814927 |
| equine g3p[3] rotavirus strain e3198 related to simian rrv and feline/canine-like rotaviruses based on complete genome analyses. | equine group a rotavirus (rva) strains are the most important cause of gastroenteritis in equine neonates and foals worldwide, and g3p[12] and g14p[12] are epidemiologically the most important genotypes. the genotype constellation of an unusual argentinean g3p[3] rva strain (rva/horse-wt/e3198/2008/g3p[3]) detected in fecal samples of a diarrheic foal in 2008 was shown to be g3-p[3]-i3-r3-c3-m3-a9-n3-t3-e3-h6. each of these genotypes has been found typically in feline and canine rva strains, and ... | 2013 | 22959604 |
| identification of an avian group a rotavirus containing a novel vp4 gene with a close relationship to those of mammalian rotaviruses. | group a rotaviruses (rvas) are an important cause of diarrhoeal illness in humans, as well as in mammalian and avian animal species. previous sequence analyses indicated that avian rvas are related only distantly to mammalian rvas. here, the complete genomes of rva strain 03v0002e10 from turkey (meleagris gallopavo) and rva strain 10v0112h5 from pheasant (phasianus colchicus) were analysed using a combination of 454 deep sequencing and sanger sequencing technologies. an adenine-rich insertion si ... | 2013 | 23052396 |
| the validation and utility of a quantitative one-step multiplex rt real-time pcr targeting rotavirus a and norovirus. | rotavirus (rov) and norovirus (nov) are the main causes of viral gastroenteritis. currently, there is no validated multiplex real-time pcr that can detect and quantify rov and nov simultaneously. the aim of the study was to develop, validate, and internally control a multiplex one-step rt real-time pcr to detect and quantify rov and nov in stool samples. pcr sensitivity was assessed by comparing amplification against the current gold standard, enzyme immunoassay (eia), on stool samples from 94 i ... | 2013 | 23046990 |
| analysis of rotavirus species diversity and evolution including the newly determined full-length genome sequences of rotavirus f and g. | rotaviruses are a leading cause of viral acute gastroenteritis in humans and animals. eight different rotavirus species (a-h) have been defined based on antigenicity and nucleotide sequence identities of the vp6 gene. here, the first complete genome sequences of rotavirus f (strain 03v0568) and g (strain 03v0567) with lengths of 18,341 and 18,186bp, respectively, are described. both viruses have open reading frames for rotavirus proteins vp1 to vp7 and nsp1 to nsp5 located at the 11 genome segme ... | 2013 | 23237956 |
| the origin of two rare human p[10] rotavirus strains. | the group a rotavirus (rva) p[10] is a rare genotype of the rva vp4 gene. to date, the whole genome sequence of only a single p[10] rva strain, rva/human-tc/idn/69m/1980/g8p4[10], has been determined, revealing a ds-1-like genotype constellation. whole genomic analyses of p[10] rva strains with other vp7 genotypes are essential to obtain conclusive data on the origin and genetic diversity of the p10] rvas. in the present study, the whole genome of a human g4p[10] rva strain, rva/human-tc/idn/57m ... | 2013 | 23178318 |
| multiplex real-time pcr assays for the detection of group c rotavirus, astrovirus, and subgenus f adenovirus in stool specimens. | group c rotavirus (gcrv), astrovirus (astv), and adenovirus (subgenus f adenov) are etiologic agents of acute nonbacterial gastroenteritis, which often represents community outbreaks. for the efficient detection of gcrv, astv, and subgenus f adenov in stool specimens, a multiplex real-time pcr assay was developed to detect these three viruses simultaneously, with high sensitivity and specificity. in total, 8404 clinical specimens were collected between april 2008 and march 2011 and tested for gc ... | 2013 | 23159674 |
| identification of circulating porcine-human reassortant g4p[6] rotavirus from children with acute diarrhea in china by whole genome analyses. | p[6] group a rotavirus (rva) strains identified in four stool specimens collected from children with acute diarrhea in guangxi province, southern china in 2010, with unknown g type were further analyzed by full genomic analysis. it was revealed by whole genome sequencing that 11 genomic cognate gene segments of these p[6] rva strains shared almost 100% nucleotide identities and all exhibited an identical g4-p[6]-i1-r1-c1-m1-a8-n1-t1-e1-h1 genotype constellation. phylogenetic analyses of vp7, vp1 ... | 2013 | 24012957 |
| circulation of group a rotaviruses among neonates of human, cow and pig: study from assam, a north eastern state of india. | rotavirus (rv) infections are worldwide in distribution causing high morbidity and mortality in human and animal neonates. human settlements in close proximity of animals aids for genetic re-assortment of the virus by interspecies transmission and consequent emergence of new viral antigenic strain. therefore, the present study was designed to explore rv incidence in a single approach from human and animal neonates sharing similar environment. altogether, 200 diarrheal samples from children (50), ... | 2013 | 24426283 |
| group a rotaviruses in children with gastroenteritis in a canadian pediatric hospital: the prevaccine era. | a publicly funded, group a rotavirus (rva) vaccination program was implemented in quebec in november 2011. | 2013 | 24421793 |
| characterization of a novel g3p[3] rotavirus isolated from a lesser horseshoe bat: a distant relative of feline/canine rotaviruses. | bats are considered important animal reservoirs for many viruses pathogenic to humans. an approach based on viral metagenomics was used to study gut specimens from 78 insectivorous bats in yunnan province, china. seventy-four reads were found to be related to group a rotavirus (rva). further reverse transcription-pcr screening and viral isolation on cell cultures confirmed the presence of a novel rva strain, named rva/bat-tc/mslh14/2012/g3p[3], in 1 (6%) of 16 lesser horseshoe bats. full genomic ... | 2013 | 24027312 |
| identification of cellular calcium binding protein calmodulin as a regulator of rotavirus a infection during comparative proteomic study. | rotavirus (rv) being the major diarrhoegenic virus causes around 527000 children death (<5 years age) worldwide. in cellular environment, viruses constantly adapt and modulate to survive and replicate while the host cell also responds to combat the situation and this results in the differential regulation of cellular proteins. to identify the virus induced differential expression of proteins, 2d-dige (two-dimensional difference gel electrophoresis) based proteomics was used. for this, ht-29 cell ... | 2013 | 23437200 |
| comparison of different rt-qpcr assays for the detection of human and bovine group a rotaviruses and characterization by sequences analysis of genes encoding vp4 and vp7 capsid proteins. | the aim of this study was to compare the performance of four rt-qpcr assays for the detection of human and bovine group a rotaviruses and to characterize the positive samples by sequence analysis of vp4 and vp7 genes. | 2013 | 23421708 |
| complex evolutionary patterns of two rare human g3p[9] rotavirus strains possessing a feline/canine-like h6 genotype on an au-1-like genotype constellation. | the group a rotavirus (rva) g3p[9] is a rare vp7-vp4 genotype combination, detected occasionally in humans and cats. other than the prototype g3p[9] strain, rva/human- tc/jpn/au-l/1982/g3p3[9], the whole genomes of only two human g3p[9] rva strains and two feline g3p[9] rva strains have been analyzed so far, revealing complex evolutionary patterns, distinct from that of au-1. we report here the whole genomic analyses of two human g3p[9] rva strains, rva/human-tc/chn/l621/2006/g3p[9] and rva/huma ... | 2013 | 23403096 |
| sequence analysis of the whole genomes of five african human g9 rotavirus strains. | the g9 rotaviruses are amongst the most common global rotavirus strains causing severe childhood diarrhoea. however, the whole genomes of only a few g9 rotaviruses have been fully sequenced and characterised of which only one g9p[6] and one g9p[8] are from africa. we determined the consensus sequence of the whole genomes of five african human group a g9 rotavirus strains, four g9p[8] strains and one g9p[6] strain collected in cameroon (central africa), kenya (eastern africa), south africa and zi ... | 2013 | 23369762 |
| detection and genetic diversity of porcine group a rotaviruses in historic (2004) and recent (2011 and 2012) swine fecal samples in ohio: predominance of the g9p[13] genotype in nursing piglets. | epidemiological surveillance of porcine group a rotavirus (rva) strains was conducted in five swine herds in ohio using historical (2004) and recent (2011 to 2012) fecal samples. of the 371 samples examined, 9.4% (35/371) were positive for rva. the rva detection rates increased from 5.9% in 2004 and 8.5% in 2011 to 13.8% in 2012. a total of 23 positive samples were analyzed for rva g and p genotypes. the dominant g-p combination was g9p[13] found in 60.9% of positive samples. the other combinati ... | 2013 | 23363823 |
| changes in anti-group a rotavirus antibody seroprevalence and levels in the western gyeongnam province of korea over 16 years. | to observe how anti-group a rotavirus antibody seropositivity rates and levels have changed in the western region of gyeongnam province, 2,030 serum samples collected at four collection periods (1989-1990, 1994-1995, 1999-2000, and 2004-2005) were tested by enzyme-linked immunosorbent assay for igg, and iga antibodies reacting to recombinant vp6 protein. the seroprevalences exhibit no regular patterns over a 16-yr period. for all four collection periods, the anti-rvp6 igg levels rose steadily du ... | 2013 | 23341712 |
| characterization of human rotaviruses circulating in iraq in 2008: atypical g8 and high prevalence of p[6] strains. | fecal samples from 976 children with gastroenteritis were collected and analyzed for group a rotavirus (rva), in three different cities in iraq between january 2008 and december 2008. rva antigen was detected in 394 (40%) of the samples, and 98 samples were available for further genotype analyses using multiplex rt-pcr and sequence analyses for untypeable strains. the g/p-genotype combination was determined for 69 samples, and 19, 2 and 8 samples remained p-untypeable, g-untypeable and g/p-untyp ... | 2013 | 23340225 |
| evolutionary time-scale of primate bocaviruses. | human bocavirus (hbov) is associated with acute gastroenteritis in humans, occurring mostly in young children and elderly people. four bocavirus genotypes (hbov1-hbov4) have been found so far. since there were no data on the contribution of hbov to gastroenteritis in russia, 1781 fecal samples collected from infants hospitalized with acute gastroenteritis in novosibirsk, russia during one year were tested for the presence of nucleic acids from hbov and three major gastrointestinal viruses (rotav ... | 2013 | 23313830 |
| rotavirus infection among sudanese children younger than 5 years of age: a cross sectional hospital-based study. | in sudan, rotavirus has been one of the important causative agents of diarrhea among children. rotavirus a is well known as the leading cause of diarrhea in young children worldwide. it was estimated to account for 41% of hospitalized cases of acute gastroenteritis among children in sub-saharan africa. this study aimed to determine the prevalence and the common clinical presentations of rotavirus a infection among sudanese children with gastroenteritis seeking management in hospitals. | 2013 | 24711878 |
| discovery and molecular characterization of a group a rotavirus strain detected in an argentinean vicuña (vicugna vicugna). | the wild vicuña (vicugna vicugna) is one of the four species of native south american camelids (sacs) in addition to the wild guanaco, and their domesticated counterparts, alpaca and llama, respectively. serological data have indicated the presence of group a rotaviruses (rva) specific antibodies in all 4 members of the sac, and so far, rva has been detected from alpacas, llamas and guanacos. a total of 59 fecal samples from healthy wild newborn and juvenile vicuñas, raised in captivity in jujuy ... | 2013 | 22877519 |
| [research progress of real-time quantitative pcr method for group a rotavirus detection]. | group a rotavirus is one of the most significant etiological agents which causes acute gastroenteritis among infants and young children worldwide. so far, several method which includes electron microscopy (em), enzyme immunoassay (eia), reverse transcription-polymerase chain reaction (rt-pcr)and real-time quantitative pcr has been established for the detection of rotavirus. compared with other methods, real-time quantitative pcr have advantages in specificity, sensitivity, genotyping and quantit ... | 2013 | 24520772 |
| [isolation and characterization of rotavirus from bat]. | bats are considered as important animal reservoirs for many pathogenic viruses to humans. the viral metagenomic analysis was performed to study gut and lung tissues of 30 insectivorous bats collected in yunnan province and 26 reads were noted to group a rotavirus (rva). further rt-pcr screening on bat samples and in vitro viral isolation on cell cultures confirmed the presence of a novel rva, named as rva/bat-tc/myas33/2013/g3p[10], in one of 30 stoliczka's trident bats. the vp7 gene of this str ... | 2013 | 24520769 |
| first molecular detection of group a rotavirus in urban and hospital sewage systems by nested-rt pcr in shiraz, iran. | group a rotaviruses are the most significant cause of acute gastroenteritis in children worldwide. rotaviruses are shed in high numbers and dispersed widely throughout bodies of water in the environment. this represents a significant health hazard for humans, mainly due to the stability of the viruses during wastewater treatment processes. this study was conducted to evaluate the prevalence of rotaviruses, to determine g genotypes of circulating rotaviruses and to assess the efficiency of rotavi ... | 2013 | 24499551 |
| complete genome analysis of a rare human g3p[9] rotavirus posing as an au-1 like strain. | we performed phylogenetic and sequence analysis by basic local alignment search tool (blast) of a complete human rotavirus (hrv) genome isolated from a hospitalized child with acute gastroenteritis in thailand. | 2013 | 24255863 |
| detection of picobirnavirus and rotavirus in diarrhoeic faecal samples of cattle and buffalo calves in mumbai metropolis, western india. | in this study 113 diarrhoeic faecal samples obtained from buffalo (n = 68) and cattle (n = 45) calves under 1 years of age were analysed in order to determine the presence of rotavirus infection and the frequency of picobirnavirus excretion. eleven (9.73%) samples positive for group a rotavirus were identified through rna-polyacrylamide gel electrophoresis (rna-page), while 4 (3.53%) samples showed a bisegmented genome with a typical picobirnavirus pattern. this is the first report of picobirnav ... | 2013 | 24362776 |
| prevalence of rotavirus genotypes in south korea in 1989-2009: implications for a nationwide rotavirus vaccine program. | the epidemiology of human group a rotavirus was analyzed by examining genotypic data acquired from 1989 to 2009 in south korea. this information was derived from all the available published articles on rotavirus studies in south korea, retrieved from both the pubmed and koreamed databases. four common g types (g1, g2, g3, and g4) and three common p types (p[8], p[4], and p[6]) accounted for approximately 93% and 99% of the rotavirus reports, respectively. the g9 type was frequently detected afte ... | 2013 | 24348658 |
| global distribution of group a rotavirus strains in horses: a systematic review. | group a rotavirus (rva) is a major cause of diarrhea and diarrhea-related mortality in foals in parts of the world. in addition to careful horse farm management, vaccination is the only known alternative to reduce the rva associated disease burden on horse farms. the precise evaluation of vaccine effectiveness against circulating strains needs enhanced surveillance of equine rvas in areas where vaccine is already available or vaccine introduction is anticipated. therefore, we undertook the overv ... | 2013 | 23994380 |
| molecular characterization of the porcine group a rotavirus nsp2 and nsp5/6 genes from são paulo state, brazil, in 2011/12. | rotaviruses are responsible for the acute diarrhea in various mammalian and avian species. the nonstructural proteins nsp2 and nsp5 are involved in the rotavirus replication and the formation of viroplasm, cytoplasmic inclusion bodies within which new viral particles morphogenesis and viral rna replication occur. there are few studies on the genetic diversity of those proteins; thus this study aims at characterizing the diversity of rotavirus based on nsp2 and nsp5 genes in rotaviruses circulati ... | 2013 | 23970830 |
| evidence of natural transmission of group a rotavirus between domestic pigs and wild boars (sus scrofa) in japan. | group a rotaviruses (rvas) are a major cause of acute dehydrating diarrhea in infants and young animals worldwide. rvas have also been detected in several wild and zoo animals, indicating wide susceptibility of wild animals. however, the role of wild animals in the infection cycle of rvas is unclear. wild boars are indigenous in many countries in the world. japanese wild boars (sus scrofa leucomystax) have been migrating close to human habitats in japan, indicating the possibility of natural tra ... | 2013 | 23958658 |
| group a rotavirus and norovirus display sharply distinct seasonal profiles in belém, northern brazil. | several viruses have been associated with acute gastroenteritis (age), and group a rotavirus (rva) and norovirus (nov) are the most prevalent. this study aimed to assess their prevalence among children hospitalised for diarrhoea during a three-year surveillance study. from may 2008-april 2011, overall positivity rates of 21.6% (628/2904) and 35.4% (171/483) were observed for rva and nov, respectively. the seasonality observed indicated distinct patterns when both viruses were compared. this find ... | 2013 | 23903985 |
| novel g10p[14] rotavirus strain, northern territory, australia. | we identified a genotype g10p[14] rotavirus strain in 5 children and 1 adult with acute gastroenteritis from the northern territory, australia. full genome sequence analysis identified an artiodactyl-like (bovine, ovine, and camelid) g10-p[14]-i2-r2-c2-m2-a11-n2-t6-e2-h3 genome constellation. this finding suggests artiodactyl-to-human transmission and strengthens the need to continue rotavirus strain surveillance. | 2013 | 23876354 |
| detection of unusual g6 rotavirus strains in italian children with diarrhoea during the 2011 surveillance season. | two rare g6 rotavirus a (rva) strains, designated as rva/human-wt/ita/cec06/2011/g6p[6] and rva/human-wt/ita/pg05/2011/g6p[9], were identified in stool specimens from children hospitalized in central italy. after pcr genotyping, the samples cec06 and pg05 gave g-ud-p[6] and g-ud-p[9] genotypes, respectively. to determine the g-type and to characterize further the two strains, sequencing of 8 of the 11 genomic segments was performed. cec06 and pg05 strains were found to possess unusual genotype c ... | 2013 | 23868788 |
| rabbit colony infected with a bovine-like g6p[11] rotavirus strain. | group a rotaviruses (rvas) are the main etiological agent of infantile diarrhea in both humans and animals worldwide. a limited number of studies have investigated the molecular characteristics of rva strains in stool specimens of rabbits, with only a few lapine rva strains isolated and (partially) characterized to date. the most common g/p-genotype combinations found in rabbits are g3p[14] and g3p[22]. in this study a rva strain was isolated from the small intestine of a 9-week-old rabbit from ... | 2013 | 23830050 |
| zoonotic transmission of reassortant porcine g4p[6] rotaviruses in hungarian pediatric patients identified sporadically over a 15 year period. | genotype g4p[6] rotavirus a (rva) strains collected from children admitted to hospital with gastroenteritis over a 15 year period in the pre rotavirus vaccine era in hungary were characterized in this study. whole genome sequencing and phylogenetic analysis was performed on eight g4p[6] rva strains. all these rva strains shared a fairly conservative genomic configuration (g4-p[6]-i1/i5-r1-c1-m1-a1/a8-n1-t1/t7-e1-h1) and showed striking similarities to porcine and porcine-derived human rva strain ... | 2013 | 23792183 |
| [genetic diversity of rotavirus group a: correlation between g3 type and severity of the infection. valencia, venezuela]. | genetic and antigenic rotavirus (rv) variabilities may have implications in the severity of the infection caused by these agents; however the studies are not conclusive. for that purpose, the mean severity scores of diarrhea episodes caused by rv types g1 and g3 were compared, at ciudad hospitalaria "dr. enrique tejera" in valencia, venezuela, between 2001-2005. rv were identified by elisa, g and p types by rt-pcr. the severity of infection was determined using the ruuska-vesikari system and the ... | 2013 | 23781711 |
| rotavirus shedding in symptomatic and asymptomatic children using reverse transcription-quantitative pcr. | reverse transcription-real-time polymerase chain reaction (rt-qpcr) for the vp6 gene was used to study group a rotavirus shedding in children with symptomatic and asymptomatic rotavirus infection. sequential stool samples (n = 345) from 10 children with rotavirus associated diarrhea and from five children (n = 161) with asymptomatic rotavirus infection were collected over a period of 2 months. a rt-qpcr assay on the samples using a rotavirus vp6 plasmid standard demonstrated high reproducibility ... | 2013 | 23775335 |
| human g9p[8] rotavirus strains circulating in cameroon, 1999-2000: genetic relationships with other g9 strains and detection of a new g9 subtype. | group a rotaviruses (rv-a) are the leading cause of viral gastroenteritis in children worldwide and genotype g9p[8] is one of the five most common genotypes detected in humans. in order to gain insight into the degree of genetic variability of g9p[8] strains circulating in cameroon, stool samples were collected during the 1999-2000 rotavirus season in two different geographic regions in cameroon (southwest and western regions). by rt-pcr, 15 g9p[8] strains (15/89=16.8%) were identified whose gen ... | 2013 | 23770141 |
| simultaneous detection of group a rotavirus in swine and rat on a pig farm in brazil. | this study investigated the occurrence of rotavirus in porcine and rattus norvegicus, at the same time, on a pig farm in the city of jaguariúna, são paulo, brazil. swine (n = 21) and rat (n = 6) fecal samples were analyzed by nested rt-pcr assay. rotavirus occurred in seven porcine and two rat samples. a total of three pig and one rat samples were further submitted to genetic sequencing. the partial nsp5 gene phylogeny showed that all strains were segregated in the genotype h1. these results poi ... | 2013 | 23766702 |
| one year survey of human rotavirus strains suggests the emergence of genotype g12 in cameroon. | in this study the emergence of rotavirus a genotype g12 in children <5 years of age is reported from cameroon during 2010/2011. a total of 135 human stool samples were p and g genotyped by reverse transcriptase pcr. six different rotavirus vp7 genotypes were detected, including g1, g2, g3, g8, g9, and g12 in combinations with p[4], p[6] and p[8] vp4 genotypes. genotype g12 predominated in combination with p[8] (54.1%) and p[6] (10.4%) genotypes followed by g1p[6] (8.2%), g3p[6] (6.7%), g2p[4] (5 ... | 2013 | 23765785 |
| phylogenetic analyses of the vp4 and vp7 genes of porcine group a rotaviruses in sao paulo state, brazil: first identification of g5p[23] in piglets. | this study determined the group a rotavirus occurrence in pig farms from 7 different cities in são paulo state, brazil. out of 143 samples, 70 tested positive. sequence analyses of 37 strains indicated that the strains had the g3, g5, g9, and p[6], p[13]/p[22]-like, and p[23] genotypes. | 2013 | 23761160 |
| molecular characterization of rotavirus genotypes in immunosuppressed and non-immunosuppressed pediatric patients. | to describe the genotypic variability of group a rotavirus (rva) found in immunosuppressed and non-immunosuppressed pediatric patients treated at the hospital de clínicas da universidade federal do paraná (hc-ufpr), curitiba, paraná. | 2013 | 23684457 |
| whole-genomic analysis of g3p[23], g9p[23] and g3p[13] rotavirus strains isolated from piglets with diarrhea in thailand, 2006-2008. | group a rotavirus (rva) is the most common cause of severe acute viral gastroenteritis in humans and animals worldwide. this study characterized the whole genome sequences of porcine rvas, 2 g3p[23] strains (cmp40/08 and cmp48/08), 1 g9p[23] strain (cmp45/08), and 1 g3p[13] strain (cmp29/08). these strains were collected from diarrheic piglets less than 7weeks of age in 4 pig farms in chiang mai, thailand, in 2008. the vp7-vp4-vp6-vp1-vp2-vp3-nsp1-nsp2-nsp3-nsp4-nsp5 genes of cmp40/08 and cmp48/ ... | 2013 | 23681022 |
| recombinant monovalent llama-derived antibody fragments (vhh) to rotavirus vp6 protect neonatal gnotobiotic piglets against human rotavirus-induced diarrhea. | group a rotavirus (rva) is the leading cause of severe diarrhea in children. the aims of the present study were to determine the neutralizing activity of vp6-specific llama-derived single domain nanoantibodies (vhh nanoabs) against different rva strains in vitro and to evaluate the ability of g6p[1] vp6-specific llama-derived single domain nanoantibodies (vhh) to protect against human rotavirus in gnotobiotic (gn) piglets experimentally inoculated with virulent wa g1p[8] rotavirus. supplementati ... | 2013 | 23658521 |
| review of group a rotavirus strains reported in swine and cattle. | group a rotavirus (rva) infections cause severe economic losses in intensively reared livestock animals, particularly in herds of swine and cattle. rva strains are antigenically heterogeneous, and are classified in multiple g and p types defined by the two outer capsid proteins, vp7 and vp4, respectively. this study summarizes published literature on the genetic and antigenic diversity of porcine and bovine rva strains published over the last 3 decades. the single most prevalent genotype combina ... | 2013 | 23642647 |
| feline origin of rotavirus strain, tunisia, 2008. | in tunisia in 2008, an unusual g6p[9] rotavirus, rva/human-wt/tun/17237/2008/g6p[9], rarely found in humans, was detected in a child. to determine the origin of this strain, we conducted phylogenetic analyses and found a unique genotype constellation resembling rotaviruses belonging to the feline ba222-like genotype constellation. the strain probably resulted from direct cat-to-human transmission. | 2013 | 23631866 |
| impact of rotavirus vaccine on rotavirus genotypes and caliciviruses circulating in french cattle. | group a rotaviruses are a leading cause of neonatal calf diarrhoea worldwide and prevention of this disease includes vaccination against these viruses. in order to highlight the potential selection of rotavirus genotypes due to immune pressure driven by vaccination, the aim of this study was to compare group a rotavirus genotypes circulating in french diarrhoeic calves in rotavirus vaccinated herds (g6p[5] vaccine) with those in non-vaccinated herds during one calving season in 2010. this study ... | 2013 | 23579256 |
| phylogenetic analysis of g1p[6] group a rotavirus strains detected in northeast brazilian children fully vaccinated with rotarix™. | in 2009 the world health organization recommended the use of group a rotavirus (rva) vaccines in all national immunization programs (nips) in order to control severe rva gastroenteritis disease. in brazil, rotarix™ was introduced in the nip in march 2006, and a significant reduction in mortality rates among children ≤ 5 years old was observed, especially in the northern and northeastern brazil. in the current study the 11 gene segments of six brazilian g1p[6] rva strains, isolated in 2009 and 20 ... | 2013 | 23538335 |
| continued circulation of g12p[6] rotaviruses over 28 months in nepal: successive replacement of predominant strains. | rotavirus a causes severe diarrhoea in infants and young children worldwide. the migration pattern (electropherotype) of the double-stranded rna genome upon polyacrylamide gel electrophoresis has been used to define "strains" in molecular epidemiology. in temperate countries, distinct electropherotypes (strains) appear after the annual, off-seasonal interruption of rotavirus circulation. in nepal, rotavirus circulated year-round and an uncommon genotype g12p[6] predominated and persisted, provid ... | 2013 | 23533063 |
| evidence for genetic reassortment between human rotaviruses by full genome sequencing of g3p[4] and g2p[4] strains co-circulating in india. | rotavirus a causes severe diarrhoea in infants and young children worldwide. many unusual combinations of g and p genotypes have been observed in rotaviruses circulating in developing countries. mixed infection of a single individual with more than one strain is a mechanism by which genetic reassortants are formed with unusual g and p combinations. however, few studies have provided direct evidence for the formation of such unusual strains as a result of co-infection of co-circulating strains. h ... | 2013 | 23532829 |
| detection of bovine group a rotavirus using rapid antigen detection kits, rt-pcr and next-generation dna sequencing. | we investigated the sensitivity of human rotavirus rapid antigen detection (rad) kits, rt-pcr and next-generation dna sequencing (ngs) for detection of bovine group a rotavirus (rva). the dipstick 'eiken' rota (dipstick) showed the highest sensitivity out of the seven rad kits against all selected strains in limited dilution analyses, which was consistent with the results of equine rotavirus previously reported. rt-pcr had 10(0)-10(3)-fold higher sensitivity than the dipstick. the ngs using rt-p ... | 2013 | 23912876 |
| evaluation of luminex xtag gastrointestinal pathogen analyte-specific reagents for high-throughput, simultaneous detection of bacteria, viruses, and parasites of clinical and public health importance. | acute diarrheal disease (add) can be caused by a range of pathogens, including bacteria, viruses, and parasites. conventional diagnostic methods, such as culture, microscopy, biochemical assays, and enzyme-linked immunosorbent assays (elisa), are laborious and time-consuming and lack sensitivity. combined, the array of tests performed on a single specimen can increase the turnaround time (tat) significantly. we validated a 19plex laboratory-developed gastrointestinal pathogen panel (gpp) using l ... | 2013 | 23850948 |
| sensitive and specific quantitative detection of rotavirus a by one-step real-time reverse transcription-pcr assay without antecedent double-stranded-rna denaturation. | a real-time quantitative reverse transcription-pcr (qrt-pcr) assay using the recombinant thermostable thermus thermophilus (rtth) enzyme was developed to detect and quantify rotavirus a (rva). by using rtth polymerase, significant improvement was achieved over the existing real-time rt-pcr assays, which require denaturation of the rva double-stranded rna (dsrna) prior to assay setup. using a dsrna transcript for segment 7, which encodes the assay target nsp3 gene, the limit of detection for the ... | 2013 | 23850952 |
| mean protein evolutionary distance: a method for comparative protein evolution and its application. | proteins are under tight evolutionary constraints, so if a protein changes it can only do so in ways that do not compromise its function. in addition, the proteins in an organism evolve at different rates. leveraging the history of patristic distance methods, a new method for analysing comparative protein evolution, called mean protein evolutionary distance (meaped), measures differential resistance to evolutionary pressure across viral proteomes and is thereby able to point to the proteins' rol ... | 2013 | 23613826 |
| histophilus somni-induced infections in cattle from southern brazil. | the sudden death of three calves, one diarrheic calf, and one aborted fetus from four farms in southern brazil was investigated. two histophilus somni-associated syndromes were identified: systemic histophilosis (n = 4) and abortion (n = 1). the principal pathological findings included vasculitis, meningoencephalitis with thrombosis, necrotizing myocarditis, renal infarctions, hepatic abscesses, and bronchopneumonia. pcr assays were used to amplify specific amplicons of the ovine herpesvirus 2, ... | 2013 | 23526124 |
| phylogenetic analyses of typical bovine rotavirus genotypes g6, g10, p[5] and p[11] circulating in argentinean beef and dairy herds. | group a rotavirus (rva) is one of the main causes of neonatal calf diarrhea worldwide. rva strains affecting argentinean cattle mainly possess combinations of the g6, g10, p[5] and p[11] genotypes. to determine rva diversity among argentinean cattle, representative bovine rva strains detected in diarrheic calves were selected from a survey conducted during 1997-2009. the survey covered the main livestock regions of the country from dairy and beef herds. different phylogenetic approaches were use ... | 2013 | 23624201 |
| g8 rotaviruses with conserved genotype constellations detected in malawi over 10 years (1997-2007) display frequent gene reassortment among strains co-circulating in humans. | rotavirus a, the most common cause of severe diarrhoea in children worldwide, occurs in five major vp7 (g) and vp4 (p) genotype combinations, comprising g1p[8], g2p[4], g3p[8], g4p[8] and g9p[8]. however, g8, a common bovine rotavirus genotype, has been reported frequently among children in african countries. surveillance of rotavirus gastroenteritis conducted in a sentinel hospital in blantyre, malawi between 1997 and 2007 provided a rare opportunity to examine the whole genotype constellation ... | 2013 | 23407423 |