Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| crystal structure of an aromatic ring opening dioxygenase ligab, a protocatechuate 4,5-dioxygenase, under aerobic conditions. | sphingomonas paucimobilis syk-6 utilizes an extradiol-type catecholic dioxygenase, the ligab enzyme (a protocatechuate 4,5-dioxygenase), to oxidize protocatechuate (or 3,4-dihydroxybenzoic acid, pca). the enzyme belongs to the family of class iii extradiol-type catecholic dioxygenases catalyzing the ring-opening reaction of protocatechuate and related compounds. the primary structure of ligab suggests that the enzyme has no evolutionary relationship with the family of class ii extradiol-type cat ... | 1999 | 10467151 |
| characterization of the ybdt gene product of bacillus subtilis: novel fatty acid beta-hydroxylating cytochrome p450. | we have characterized the gene encoding fatty acid alpha-hydroxylase, a cytochrome p450 (p450) enzyme, from sphingomonas paucimobilis. a database homology search indicated that the deduced amino acid sequence of this gene product was 44% identical to that of the ybdt gene product that is a 48 kda protein of unknown function from bacillus subtilis. in this study, we cloned the ybdt gene and characterized this gene product using a recombinant enzyme to clarify function of the ybdt gene product. th ... | 1999 | 10529095 |
| crystallization and preliminary x-ray diffraction analysis of haloalkane dehalogenase linb from sphingomonas paucimobilis ut26. | haloalkane hydrolytic dehalogenase linb from sphingomonas paucimobilis ut26, an enzyme which releases chloride or bromide anion from n-halogenated alkanes and has a broad range of substrate specificity, was crystallized using the hanging-drop vapour-diffusion method at 278 k. the best crystals were obtained by microseeding with a precipitant containing 18-20%(w/v) peg 6000, 0.2 m calcium acetate and 0.1 m tris-hcl ph 8.9. the crystals diffract to at least 1.60 a using synchrotron x-ray under cry ... | 1999 | 10329794 |
| survival of enterobacter cloacae and pseudomonas paucimobilis in yoghurts manufactured from cow's milk and soymilk during storage at different temperatures. | the survival of two microbial contaminants, enterobacter cloacae and pseudomonas paucimobilis, in yoghurts manufactured from cow's milk and soymilk was investigated during storage for 45 days at 4 and 12 degrees c. sensory panel tests performed before microbiological investigation, showed that the flavor of soy-yoghurts made with cocoa powder or malt added did not have the beany taste of soy beans. both contaminants were significantly resistant to low ph values during storage for 32 days at 4 de ... | 1999 | 10356792 |
| genetic and biochemical characterization of a 2-pyrone-4, 6-dicarboxylic acid hydrolase involved in the protocatechuate 4, 5-cleavage pathway of sphingomonas paucimobilis syk-6. | sphingomonas paucimobilis syk-6 is able to grow on a wide variety of dimeric lignin compounds with guaiacyl moieties, which are converted into protocatechuate by the actions of lignin degradation enzymes in this strain. protocatechuate is a key metabolite in the syk-6 degradation of lignin compounds with guaiacyl moieties, and it is thought that it degrades to pyruvate and oxaloacetate via the protocatechuate 4,5-cleavage pathway. in a 10.5-kb ecori fragment carrying the protocatechuate 4,5-diox ... | 1999 | 9864312 |
| complement activation by bacterial surface glycolipids: a study with planar bilayer membranes. | planar asymmetric glycolipid/phospholipid bilayer membranes were used as a reconstitution model of the lipid matrix of the outer membrane of gram-negative bacteria to study complement (c) activation by various bacterial surface glycolipids with the aim of defining the c activation pathway. as glycolipids the lipopolysaccharides of salmonella enterica serovar minnesota r mutant strains r595 (re lps) and r4 (rd2 lps), pentaacyl lipid a from the lps of the escherichia coli re mutant f515, and glyco ... | 1999 | 9929374 |
| identification of the catalytic triad in the haloalkane dehalogenase from sphingomonas paucimobilis ut26. | the haloalkane dehalogenase from sphingomonas paucimobilis ut26 (linb) is the enzyme involved in the gamma-hexachlorocyclohexane degradation. this enzyme hydrolyses a broad range of halogenated aliphatic compounds via an alkyl-enzyme intermediate. linb is believed to belong to the family of alpha/beta-hydrolases which employ a catalytic triad, i.e. nucleophile-histidine-acid, during the catalytic reaction. the position of the catalytic triad within the sequence of linb was probed by a site-direc ... | 1999 | 10100638 |
| two different types of dehalogenases, lina and linb, involved in gamma-hexachlorocyclohexane degradation in sphingomonas paucimobilis ut26 are localized in the periplasmic space without molecular processing. | gamma-hexachlorocyclohexane (gamma-hch) is one of several highly chlorinated insecticides that cause serious environmental problems. the cellular proteins of a gamma-hch-degrading bacterium, sphingomonas paucimobilis ut26, were fractionated into periplasmic, cytosolic, and membrane fractions after osmotic shock. most of two different types of dehalogenase, lina (gamma-hexachlorocyclohexane dehydrochlorinase) and linb (1,3,4,6-tetrachloro-1,4-cyclohexadiene halidohydrolase), that are involved in ... | 1999 | 10464214 |
| [bacteremia by sphingomonas paucimobilis]. | 1998 | 9586371 | |
| cloning of a sphingomonas paucimobilis syk-6 gene encoding a novel oxygenase that cleaves lignin-related biphenyl and characterization of the enzyme. | sphingomonas paucimobilis syk-6 transforms 2,2'-dihydroxy-3,3'-dimethoxy-5,5'-dicarboxybiphenyl (ddva), a lignin-related biphenyl compound, to 5-carboxyvanillic acid via 2,2',3-trihydroxy-3'-methoxy-5,5'-dicarboxybiphenyl (oh-ddva) as an intermediate (15). the ring fission of oh-ddva is an essential step in the ddva degradative pathway. a 15-kb ecori fragment isolated from the cosmid library complemented the growth deficiency of a mutant on oh-ddva. subcloning and deletion analysis showed that a ... | 1998 | 9647824 |
| alpha-l-rhamnosidase of sphingomonas sp. r1 producing an unusual exopolysaccharide of sphingan. | a soil bacterium with alpha-l-rhamnosidase was isolated from a cumulative mixed culture containing a polysaccharide of gellan as a carbon source and identified to be sphingomonas paucimobilis, known as a potent producer of gellan. the isolate (designated sphingomonas sp. r1) produced an unusual exopolysaccharide of sphingan (denoted hwr1) distinct from gellan. the rhamnose in gellan was replaced with mannose in hwr1. the bacterium had a peculiar cell surface covered with many complicated plaits. ... | 1998 | 9692187 |
| impact of inoculation protocols, salinity, and ph on the degradation of polycyclic aromatic hydrocarbons (pahs) and survival of pah-degrading bacteria introduced into soil. | degradation of polycyclic aromatic hydrocarbons (pahs) and survival of bacteria in soil was investigated by applying different inoculation protocols. the soil was inoculated with sphingomonas paucimobilis ba 2 and strain bp 9, which are able to degrade anthracene and pyrene, respectively. cfu of soil bacteria and of the introduced bacteria were monitored in native and sterilized soil at different phs. introduction with mineral medium inhibited pah degradation by the autochthonous microflora and ... | 1998 | 9435090 |
| characterization of marine temperate phage-host systems isolated from mamala bay, oahu, hawaii. | to understand the ecological and genetic role of viruses in the marine environment, it is critical to know the infectivity of viruses and the types of interactions that occur between marine viruses and their hosts. we isolated four marine phages from turbid plaques by using four indigenous bacterial hosts obtained from concentrated water samples from mamala bay, oahu, hawaii. two of the rod-shaped bacterial hosts were identified as sphingomonas paucimobilis and flavobacterium sp. all of the phag ... | 1998 | 9464390 |
| molecular mechanisms of polymyxin b-membrane interactions: direct correlation between surface charge density and self-promoted transport. | we have studied the interaction of the polycationic peptide antibiotic polymyxin b (pmb) with asymmetric planar bilayer membranes via electrical measurements. the bilayers were of different compositions, including those of the lipid matrices of the outer membranes of various species of gram-negative bacteria. one leaflet, representing the bacterial inner leaflet, consisted of a phospholipid mixture (pl; phosphatidylethanolamine, -glycerol, and diphosphatidylglycerol in a molar ratio of 81:17:2). ... | 1998 | 9538506 |
| effect of calcium on the surfactant tolerance of a fluoranthene degrading bacterium. | surfactants are known to increase the apparent aqueous solubility of polycyclic aromatic hydrocarbons (pahs) and may thus be used to enhance the bioavailability and thereby to stimulate the biodegradation of these hydrophobic compounds. however, surfactants may in some cases reduce or inhibit biodegradation because of toxicity to the bacteria. in this study, toxicity of surfactants on sphingomonas paucimobilis strain epa505 and the effect on fluoranthene mineralization were investigated using tr ... | 1998 | 10192897 |
| phytanic acid alpha-hydroxylation by bacterial cytochrome p450. | fatty acid alpha-hydroxylase, a cytochrome p450 enzyme, from sphingomonas paucimobilis, utilizes various straight-chain fatty acids as substrates. we investigated whether a recombinant fatty acid alpha-hydroxylase is able to metabolize phytanic acid, a methyl-branched fatty acid. when phytanic acid was incubated with the recombinant enzyme in the presence of h2o2, a reaction product was detected by gas chromatography, whereas a reaction product was not detected in the absence of h2o2. when a hea ... | 1998 | 9930407 |
| microbial contamination of 'sterile water' used in japanese hospitals. | we examined the following samples of water from 10 hospitals for microbial contamination: water obtained using an ultra filtration system (uf water), a reverse osmosis system (ro water), a water distillation system (distilled water) and tap water. uf water and ro water are used for handwashing before surgery, and distilled water for the preparation of drugs. all 10 samples of tap water examined were contaminated with < 10 colony forming units (cfu)/ml. thirteen (68%) of 19 samples of uf water, n ... | 1998 | 9513069 |
| cloning and sequencing of a 2,5-dichlorohydroquinone reductive dehalogenase gene whose product is involved in degradation of gamma-hexachlorocyclohexane by sphingomonas paucimobilis. | sphingomonas (formerly pseudomonas) paucimobilis ut26 utilizes gamma-hexachlorocyclohexane (gamma-hch), a halogenated organic insecticide, as a sole carbon and energy source. in a previous study, we showed that gamma-hch is degraded to 2,5-dichlorohydroquinone (2,5-dchq) (y. nagata, r. ohtomo, k. miyauchi, m. fukuda, k. yano, and m. takagi, j. bacteriol. 176:3117-3125, 1994). in the present study, we cloned and characterized a gene, designated lind, directly involved in the degradation of 2,5-dc ... | 1998 | 9515900 |
| cloning and sequencing of the sphingomonas (pseudomonas) paucimobilis gene essential for the o demethylation of vanillate and syringate. | sphingomonas (pseudomonas) paucimobilis syk-6 is able to grow on 5,5'-dehydrodivanillic acid (ddva), syringate, vanillate, and other dimeric model compounds of lignin as a sole carbon source. nitrosoguanidine mutagenesis of s. paucimobilis syk-6 was performed, and two mutants with altered ddva degradation pathways were isolated. the mutant strain nt-1 could not degrade ddva, but could degrade syringate, vanillate, and 2,2',3'-trihydroxy-3-methoxy-5,5'-dicarboxybiphenyl (oh-ddva). strain dc-49 co ... | 1998 | 9501423 |
| nosocomial infections caused by sphingomonas paucimobilis: clinical features and microbiological characteristics. | from january 1995 to september 1996, 14 isolates of sphingomonas paucimobilis, including 11 from clinical specimens from six patients with nosocomial infection and three from environmental sources, were collected. two of the six patients had intravascular catheter-related bacteremia and one each had bacteremic biliary tract infection, urinary tract infection, ventilator-associated pneumonia, and wound infection. the s. paucimobilis isolates were identified according to biochemical profiles estab ... | 1998 | 9524843 |
| oxidation of methyl-substituted naphthalenes: pathways in a versatile sphingomonas paucimobilis strain | aromatic compounds with alkyl substituents are abundant in fossil fuels. these compounds become important environmental sources of soluble toxic products, developmental inhibitors, etc. principally through biological activities. to assess the effect of methyl substitution on the completeness of mineralization and accumulation of pathway products, an isolate from a phenanthrene enrichment culture, sphingomonas paucimobilis 2322, was used. washed cell suspensions containing cells grown on 2,6-dime ... | 1998 | 9572967 |
| further characterization of hydrogen peroxide-dependent fatty acid alpha-hydroxylase from sphingomonas paucimobilis. | although fatty acid alpha-hydroxylase (faah) activity has been detected in various species, faah has not been sufficiently characterized. in this report, we describe the properties of faah highly purified from sphingomonas paucimobilis. the faah was purified by about 5,200-fold. blotting analysis with a specific antibody against the faah showed that its apparent molecular mass was approximately 43 kda. faah showed alpha-hydroxylation activity in the presence of h2o2, but little if any activity w ... | 1998 | 9644252 |
| [bacteremia caused by sphingomonas (pseudomonas) paucimobilis]. | 1998 | 9835164 | |
| emergence of cu(++)-tolerant mutants defective in gellan synthesis in cu(++)-stressed cultures of sphingomonas paucimobilis. | cells defective in gellan synthesis appeared during cultivation of the gellan gum-producing strain sphingomonas paucimobilis r40 with inhibitory concentrations of copper, supplied as cucl2. the percentage of less mucoid colonial variants dramatically increased with the increase in cu++ supplementation, reaching 85% of total viable cells at the maximal concentration for growth. results reported in this work indicate that emergence of colonial variants defective in gellan synthesis results from cu ... | 1997 | 9765805 |
| glutathione s-transferase-encoding gene as a potential probe for environmental bacterial isolates capable of degrading polycyclic aromatic hydrocarbons. | homologs of the glutathione s-transferase (gst)-encoding gene were identified in a collection of aromatic hydrocarbon-degrading sphingomonas spp. isolated from new zealand, antarctica, and the united states by using pcr primers designed from the gst-encoding gene of sphingomonas paucimobilis epa505. sequence analysis of pcr fragments generated from these isolates and of the gst gene amplified from dna extracted from polycyclic aromatic hydrocarbon (pah)-contaminated soil revealed a high degree o ... | 1997 | 9251217 |
| molecular cloning and expression of fatty acid alpha-hydroxylase from sphingomonas paucimobilis. | fatty acid alpha-hydroxylase (faah) catalyzes the initial reaction in alpha-oxidation of fatty acid to produce 2-hydroxy fatty acid. faah activity has been detected in a wide range of organisms from prokaryotes to eukaryotes. here, we describe cloning of the faah gene from sphingomonas paucimobilis, a sphingolipid- and 2-hydroxymyristic acid-rich bacterium. the isolated gene encoded 415 amino acids. a homology search revealed that amino acid sequences highly conserved in cytochrome p450 (p450) w ... | 1997 | 9295298 |
| purification and some properties of lignostilbene-alpha, beta-dioxygenase isozyme iv from pseudomonas paucimobilis tmy1009. | lignostilbene-alpha, beta-dioxygenase isozyme iv was purified from ultrasonic extracts of pseudomonas paucimobilis tmy1009 through four steps of column chromatography. the fraction obtained gave a single band on sds-page and a single peak on reversed-phase hplc and deae-hplc. the specific activity and km of purified isozyme iv were 110 mu kat/g and 4.2 microm for 4.4'-dihydroxy-3,3'-dimethoxystilbene, 150 mu kat/g and 3.3 microm for 4,2'-dihydroxy-3,3'-dimethoxy-5'-(2"-carboxyvinyl)stilbene. the ... | 1997 | 9339561 |
| purification and characterization of a haloalkane dehalogenase of a new substrate class from a gamma-hexachlorocyclohexane-degrading bacterium, sphingomonas paucimobilis ut26. | the linb gene product (linb), 1,3,4,6-tetrachloro-1,4-cyclohexadiene halidohydrolase, which is involved in the degradation of gamma-hexachlorocyclohexane in sphingomonas paucimobilis ut26 (y. nagata, t. nariya, r. ohtomo, m. fukuda, k. yano, and m. takagi, j. bacteriol. 175:6403-6410, 1993), was overproduced in e. coli and purified to homogeneity. the molecular mass of linb was deduced to be 30 kda by gel filtration chromatography and 32 kda by electrophoresis on sodium dodecyl sulfate-polyacryl ... | 1997 | 9293022 |
| phylogenetic and physiological comparisons of pah-degrading bacteria from geographically diverse soils. | the diversity of bacteria isolated from creosote- contaminated soils in the united states, norway, and germany was determined by comparing their ability to degrade polycyclic aromatic hydrocarbons (pahs), their phospholipid ester-linked fatty acid (gc-fame) profiles, sole carbon source utilization patterns (biolog assays), and 16s rrna sequences. bacteria were initially obtained by enrichment with phenanthrene and fluoranthene. many were capable of degrading a broad range of the pahs found in cr ... | 1997 | 9195008 |
| bacterial degradation of low concentrations of phenanthrene and inhibition by naphthalene | phenanthrene-degrading bacteria were isolated from enrichment cultures of soils contaminated with creosote and jet fuel. the isolates from the creosote enrichments were classified by fatty acid methyl ester profiles as acidovorax delafieldii and sphingomonas paucimobilis; the bacterium from the jet fuel-contaminated soil was not identified and was designated strain jfd11. all three isolates used phenanthrene as a sole carbon and energy source, and two of the isolates used fluoranthene as a sole ... | 1996 | 8661535 |
| halopicolinic acids, novel products arising through the degradation of chloro- and bromo-biphenyl by sphingomonas paucimobilis bpsi-3. | sphingomonas paucimobilis bpsi-3 was previously isolated from a mixed microbial consortium growing on biphenyl as the sole source of carbon and energy. transformation of 4-chlorobiphenyl (4cbp) was demonstrated by this strain, although little or no growth was observed. in minimal salts medium supplemented with 4cbp or bromobiphenyl and dextrose, yellow coloured product(s) were rapidly formed. gas chromatography-mass spectrometry (gc-ms) revealed single-ring n-heterocyclic compounds that were ide ... | 1996 | 8595598 |
| novel polypeptides induced by the insecticide lindane (gamma-hexachlorocyclohexane) are required for its biodegradation by a sphingomonas paucimobilis strain. | when exposed to the potent insecticide gamma-hexachlorocyclohexane or lindane, a sphingomonas paucimobilis strain rapidly synthesized 7 novel polypeptides and concomitantly gained the ability to degrade lindane. synthesis of these proteins was switched-off subsequent to the disappearance of lindane from the medium. treatments which induced the synthesis of identical proteins also conferred on cells the ability to degrade lindane. in contrast, cells blocked in protein synthesis could not be induc ... | 1996 | 8630034 |
| tracheal colonization with sphingomonas paucimobilis in mechanically ventilated neonates due to contaminated ventilator temperature probes. | sphingomonas paucimobilis was isolated from tracheal secretions of a total of 85 mechanically ventilated babies in a neonatal intensive-care unit (nicu) during a two-year-period. none of the neonates developed pneumonia or sepsis. after each increase in the fluctuating number of s. paucimobilis isolates, extra attention was paid to hand hygiene and to the maintenance of the ventilation equipment. this resulted in a reduction of the frequency of isolation each time. cultures of all liquids in use ... | 1996 | 8690883 |
| bacteremia due to glucose non-fermenting gram-negative bacilli in patients with hematological neoplasias and solid tumors. | twenty-six patients with hematological or solid tumors who developed bacteremia caused by stenotrophomonas maltophilia (n = 10), pseudomonas putida (n = 6), sphingomonas paucimobilis complex (n = 4) or alcaligenes xylosoxidans (n = 6) in the period between 1993 and 1995 were studied. seventeen patients were neutropenic during the infection, and 13 were undergoing bone marrow or peripheral blood stem cell transplantation. twenty-three patients had catheter-related infections; only 3 of the 26 pat ... | 1996 | 8874083 |
| planar asymmetric lipid bilayers of glycosphingolipid or lipopolysaccharide on one side and phospholipids on the other: membrane potential, porin function, and complement activation. | we have determined some physicochemical properties of the monosaccharide-type fraction (gsl-1) of glycosphingolipids, the major glycolipid components of the outer leaflet of the gram-negative species sphingomonas paucimobilis. these properties included the state of order of the hydrocarbon moiety, the effective molecular area, surface charge density, and intrinsic transmembrane potential profile of reconstituted planar asymmetric gsl-1/phospholipid bilayer membranes. we have, furthermore, invest ... | 1996 | 8770208 |
| isolation and characterization of a novel gamma-hexachlorocyclohexane-degrading bacterium. | the natural biotic capacity of soils to degrade gamma-hexachlorocyclohexane (gamma-hch, lindane) was estimated using an enrichment technique based on the ability of soil bacteria to develop on synthetic media and degrade the xenobiotic compound, used as the sole source of carbon and energy. bacterial inocula from relatively highly contaminated soils (from wood treatment factories) were found to promote efficiently the degradation of gamma-hch, which subsequently permitted isolation of a competen ... | 1996 | 8830705 |
| structural studies of the exocellular polysaccharide from sphingomonas paucimobilis strain i-886. | the exocellular polysaccharide from sphingomonas paucimobilis strain i-886 has been studied using methylation analysis, smith degradation, partial acid hydrolysis, nmr spectroscopy, and mass spectrometry as the principal methods. it is concluded that the repeating unit has the following structure: [formula: see text] the absolute configuration of the uronic acid was deduced from 1h nmr chemical shifts and is most likely d. some preparations of the polysaccharide also contain phosphate and o-acyl ... | 1996 | 9011378 |
| catechol dioxygenases from escherichia coli (mhpb) and alcaligenes eutrophus (mpci): sequence analysis and biochemical properties of a third family of extradiol dioxygenases. | the nucleotide sequence of the escherichia coli mhpb gene, encoding 2,3-dihydroxyphenylpropionate 1,2-dioxygenase, was determined by sequencing of a 3.1-kb fragment of dna from kohara phage 139. the inferred amino acid sequence showed 58% sequence identity with the sequence of an extradiol dioxygenase, mpci, from alcaligenes eutrophus and 10 to 20% sequence identity with protocatechuate 4,5-dioxygenase from pseudomonas paucimobilis, with 3,4-dihydroxyphenylacetate 2,3-dioxygenase from e. coli, a ... | 1996 | 8752345 |
| description of chlorophenol-degrading pseudomonas sp. strains kf1t, kf3, and nkf1 as a new species of the genus sphingomonas, sphingomonas subarctica sp. nov. | gram-negative polychlorophenol-degrading bacterial strains kf1t (t = type strain), kf3, and nkf1, which were described previously as pseudomonas saccharophila strains, were studied by chemotaxonomic, genetic, and physiological methods and by electron microscopy and compared with selected xenobiotic compound-degrading bacteria. these strains contained sphingolipids with d-18:0, d-20:1, and d-21:1 as the main dihydrosphingosines, ubiquinone 10 as the main respiratory quinone, and spermidine as the ... | 1996 | 8863434 |
| degradation of diclofop-methyl by pure cultures of bacteria isolated from manitoban soils. | pure cultures of chryseomonas luteola and sphingomonas paucimobilis isolated from manitoban soils were able to utilize diclofop-methyl (methyl-2-[4-(2,4-dichlorophenoxy)phenoxy] propanoate) as the sole source of carbon and energy. an actively growing culture of c. luteola completely degraded 1.5 micrograms diclofop-methyl.ml-1 to diclofop acid and 4-(2,4-dichlorophenoxy)phenol within 71 h, as determined by gas chromatographic analysis. the accumulation of these metabolites in the growth medium r ... | 1996 | 8868229 |
| isolation and characterization of soil microorganisms capable of utilizing the herbicide diclofop-methyl as a sole source of carbon and energy. | six nonfermentative gram-negative bacilli were isolated from manitoban soils after enrichment with diclofopmethyl. microscopic examination and physiological and biochemical tests have identified the organisms as sphingomonas paucimobilis, acinetobacter baumannii, chryseomonas luteola, pseudomonas aureofaciens, pseudomonas cepacia, and pseudomonas fluorescens. growth curve studies showed that each of the isolates was able to grow in minimal medium with diclofop-methyl as the sole source of carbon ... | 1996 | 8868228 |
| computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral water. | the microflora of a natural mineral water was studied immediately after bottling (t0) and after 7 d storage (t7) during 6 months, and isolates were clustered by sds-page of whole-cell protein profiles. isolates from each cluster were further characterized by api 20ne, fatty acid composition and quinone profiles. the numerical analysis of the electrophoregrams of all bacteria isolated from the mineral water formed 15 clusters and five unclustered strains. except for five minor clusters, all clust ... | 1996 | 9072519 |
| direct involvement of hydrogen peroxide in bacterial alpha-hydroxylation of fatty acid. | we have reported that fatty-acid alpha-hydroxylase partially purified from sphingomonas paucimobilis required nadh and molecular oxygen. in this study, we found that the reaction was greatly inhibited by catalase. glutathione and glutathione peroxidase also inhibited alpha-hydroxylation, but superoxide dismutase and mannitol did not. replacement of nadh and molecular oxygen by hydrogen peroxide increased the alpha-hydroxylation activity. in the presence of hydrogen peroxide, molecular oxygen was ... | 1996 | 8647293 |
| kinetics of chromate reduction during naphthalene degradation in a mixed culture. | a mixed culture of bacillus sp. k1 and sphingomonas paucimobilis epa 505 was exposed to chromate and naphthalene. batch experiments showed that chromate was reduced and naphthalene was degraded by the mixed culture. chromate reduction occurred initially at a high rate followed by a decrease in rate until chromate reduction ceased. chromate reduction decreased in the mixed culture when a lower ratio of s. paucimobilis epa 505 to bacillus sp. k1 was utilized. a kinetic model incoporating a term fo ... | 1996 | 18629905 |
| studies on a viscous, gel-forming exopolysaccharide from sphingomonas paucimobilis gs1. | a new strain, sphingomonas paucimobilis gs1, accumulated 6.5 g of a highly viscous exopolysaccharide per liter, using sucrose as a substrate. the anionic heteropolysaccharide contained the following, in grams per gram: glucose, 0.7; galacturonic acid, 0.11; glucuronic acid, 0.07; and acetate, 0.12. the viscosity of the exopolysaccharide (4.0 g/liter; 4,200 cp) was 5.5 times that of xanthan gum and was stable over a wide ph and temperature range as well as in the presence of nacl. deacetylated po ... | 1995 | 16534966 |
| stimulation of phagocytosis and phagosome-lysosome fusion by glycosphingolipids from sphingomonas paucimobilis. | sphingomonas paucimobilis, a gram-negative opportunistic pathogen, is actively phagocytosed by human peripheral polymorphonuclear leukocytes (pmn) in vitro. however, when live or killed cells were delipidated, the phagocytic rate was clearly decreased. therefore, we have investigated the physiological role of membrane lipids in phagocytic processes. s. paucimobilis type strain 2395 produces four classes of acidic glycosphingolipids (gl-1, gl-2, gl-3, and gl-4) with the common components of glucu ... | 1995 | 8543558 |
| self-induced bacteremia. case report. | we describe a patient with perforated appendicitis who postoperatively suffered repeated episodes of shaking chills and temperature spikes. initial blood cultures yielded growth of flavobacterium meningosepticum, pseudomonas putida and pseudomonas paucimobilis, and succeeding blood cultures growth of pseudomonas acidoverans. these bacteria in combination led to a suspicion of self-inoculation of contaminated water through an intravenous catheter. antibiotic treatment had no effect on the symptom ... | 1995 | 7546651 |
| catheter-related bacteremia due to pseudomonas paucimobilis in neutropenic cancer patients: report of two cases. | 1995 | 7548522 | |
| screening and characterization of microorganisms with glutaryl-7adca acylase activity. | a screening of microorganisms producing glutaryl-7adca acylase, an enzyme able to hydrolyse glutaric acid selectively from glutaryl-3-deacetoxy-7-aminocephalosporanic acid (glutaryl-7adca), has been carried out in soil samples. five microorganisms expressing acylase activity were isolated and classified as bacillus cereus, achromobacter xylosooxidans, bacillus sp., pseudomonas sp. and pseudomonas paucimobilis. the screening was carried out by preparing enrichment cultures containing glutaryl-7-a ... | 1995 | 7632401 |
| glycosphingolipids from sphingomonas paucimobilis induce monokine production in human mononuclear cells. | glycosphingolipids (gsl) isolated from the gram-negative lipopolysaccharide (lps)-free bacterium sphingomonas paucimobilis have remarkable structural similarities with lps and its hydrophobic part, termed lipid a. like lps, but in contrast to the structurally related ceramides and cerebrosides, gsl contain an alpha-linked, negatively charged pyranosidic glycosyl component adjacent to the lipid portion and are capable of forming membranes. because of these similarities, it was of interest to inve ... | 1995 | 7542635 |
| aromatic-degrading sphingomonas isolates from the deep subsurface. | an obligately aerobic chemoheterotrophic bacterium (strain f199) previously isolated from southeast coastal plain subsurface sediments and shown to degrade toluene, naphthalene, and other aromatic compounds (j. k. fredrickson, f. j. brockman, d. j. workman, s. w. li, and t. o. stevens, appl. environ. microbiol. 57:796-803, 1991) was characterized by analysis of its 16s rrna nucleotide base sequence and cellular lipid composition. strain f199 contained 2-oh14:0 and 18:1 omega 7c as the predominan ... | 1995 | 7544095 |
| pyrimidine nucleoside catabolism in sphingomonas paucimobilis: role of cytidine deaminase and uridine phosphorylase. | pyrimidine nucleoside catabolism in the human pathogen sphingomonas paucimobilis was studied. it was observed that s. paucimobilis was only capable of utilizing cytidine or deoxycytidine as a sole nitrogen source when glucose served as the carbon source. thinlayer chromatographic analyses of cytidine and uridine catabolic products revealed that the enzymes cytidine deaminase and uridine phosphorylase were active in the extracts prepared from s. paucimobilis cells. the levels of cytidine deaminas ... | 1995 | 7600008 |
| microbiological degradation of the herbicide dicamba. | pseudomonas paucimobilis was isolated from a consortium which was capable of degrading dicamba (3,6-dichloro-2-methoxybenzoic acid) as the sole source of carbon. the degradation of dicamba by p. paucimobilis and the consortium was examined over a range of substrate concentration, temperature, and ph. in the concentration range of 100-2000 mg dicamba l-1 (0.5-9.0 mm), the degradation was accompanied by a stoichiometric release of 2 mol of cl- per mol of dicamba degraded. the cultures had an optim ... | 1995 | 7612214 |
| cloning of a lignostilbene-alpha,beta-dioxygenase isozyme gene from pseudomonas paucimobilis tmy1009. | a genomic dna library of pseudomonas paucimobilis tmy1009 was constructed using a cosmid vector pwe15. screening of the library for lignostilbene-alpha,beta-dioxygenase (lsd) isozyme genes was done with a common probe for the alpha, beta, and gamma subunits, which composed lsd isozymes. the positive clones obtained by colony hybridization were further confirmed by southern hybridization. a 4.2-kb bamhi-hindiii fragment hybridized with the probe was subcloned into puc118 to yield the plasmid pkhe ... | 1995 | 8534977 |
| characterization of pseudomonas paucimobilis fp2001 which forms flagella depending upon the presence of rhamnose in liquid medium. | a bacterial strain fp2001 isolated from the exudate of land reclaimed for municipal waste was identified as pseudomonas paucimobilis. cells of strain fp2001 were mobile by means of polar monotrichous flagellum, only when rhamnose was added as a carbon source in the liquid medium. the replacement of rhamnose by arabinose, galactose, glucose or xylose did not lead to the formation of flagella. | 1995 | 8569527 |
| bacterial contamination of cellular blood components. a retrospective review at a large cancer center. | concern over increased bacterial contamination prompted us to conduct a retrospective review of all bacterial cultures performed on cellular blood components at our institution between december 1989 and june 1993. sterility checks were accomplished by using the bactec blood culture system. the breakdown of the units cultured versus units produced was as follows: packed red blood cells (prbcs): 626 (0.6 percent)/102,593; random donor platelets (rdps): 523 (0.6 percent)/95,005; and single donor pl ... | 1995 | 7668812 |
| gellan lyases--novel polysaccharide lyases. | a number of bacterial strains capable of degrading the bacterial exopolysaccharide gellan have been isolated by standard enrichment procedures. they include several pink-pigmented gram-negative rod-shaped bacteria. a red-pigmented gram-positive bacillus earlier found to degrade the exopolysaccharide xanthan from xanthomonas campestris also showed slight gellanase activity. all the gram-negative bacteria are non-fermentative, motile and amylase-producing. the gellan degradation in each case is du ... | 1994 | 7812440 |
| structure and physical properties of the extracellular polysaccharide ps-p4 produced by sphingomonas paucimobilis p4 (dsm 6418). | a new strain, sphingomonas paucimobilis p4 (dsm 6418), was found during a screening programme for exopolysaccharide-producing bacteria. the highly viscous fermentation broth yields a polysaccharide (up to 10 kg/m3), named ps-p4, and shows thixotropic flow behaviour. in the presence of phosphate ions, ps-p4 forms aqueous gels after heating and cooling at alkaline ph. after isolation and purification of the exopolysaccharide, structural analysis by 1d and 2d 1h nmr spectroscopy and mass spectromet ... | 1994 | 8149377 |
| the cell envelope structure of the lipopolysaccharide-lacking gram-negative bacterium sphingomonas paucimobilis. | from the cell envelope preparation of sphingomonas paucimobilis two membrane fractions with different densities were separated by sucrose density gradient ultracentrifugation. the high-density fraction contained several major proteins, phospholipids, and glycosphingolipids, which are the only glycolipids of this lipopolysaccharide-lacking gram-negative bacterium. the low-density fraction showed many minor bands of proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and nadh ox ... | 1994 | 8288520 |
| use of gene probes to aid in recovery and identification of functionally dominant 2,4-dichlorophenoxyacetic acid-degrading populations in soil. | the herbicide 2,4-dichlorophenoxyacetic acid (2,4-d) was applied to soils in microcosms, and degradation was monitored after each of five repeated additions. total dnas were isolated from soil bacterial communities after each 2,4-d treatment. the dna samples were analyzed on slot blots and southern blots by using a tfda gene probe subcloned from plasmid pjp4 and a spa probe derived from a different 2,4-d-degrading isolate, a sphingomonas paucimobilis strain. 2,4-d applied to soil was quickly deg ... | 1994 | 8017908 |
| genetic and phenotypic diversity of 2,4-dichlorophenoxyacetic acid (2,4-d)-degrading bacteria isolated from 2,4-d-treated field soils. | forty-seven numerically dominant 2,4-dichlorophenoxyacetic acid (2,4-d)-degrading bacteria were isolated at different times from 1989 through 1992 from eight agricultural plots (3.6 by 9.1 m) which were either not treated with 2,4-d or treated with 2,4-d at three different concentrations. isolates were obtained from the most dilute positive most-probable-number tubes inoculated with soil samples from the different plots on seven sampling dates over the 3-year period. the isolates were compared b ... | 1994 | 8017907 |
| analysis of competition in soil among 2,4-dichlorophenoxyacetic acid-degrading bacteria. | competition among indigenous and inoculated 2,4-dichlorophenoxyacetic acid (2,4-d)-degrading bacteria was studied in a native kansas prairie soil following 2,4-d additions. the soil was inoculated with four different 2,4-d-degrading strains at densities of 10(3) cells per g of soil; the organisms used were pseudomonas cepacia dbo1(pjp4) and three michigan soil isolates, strain 745, sphingomonas paucimobilis 1443, and pseudomonas pickettii 712. following 2,4-d additions, total soil dna was extrac ... | 1994 | 8017909 |
| successful treatment of pseudomonas paucimobilis haemodialysis catheter-related sepsis without catheter removal. | infection is the most common complication of long-term central venous access lines. we report a case of central venous haemodialysis catheter infection with pseudomonas paucimobilis, successfully treated by prolonged antibiotic administration without catheter removal. the implications are discussed. | 1994 | 8140021 |
| cloning and sequencing of a 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase gene involved in the degradation of gamma-hexachlorocyclohexane in pseudomonas paucimobilis. | in pseudomonas paucimobilis ut26, gamma-hexachlorocyclohexane (gamma-hch) is converted to 2,5-dichloro-2,5-cyclohexadiene-1,4-diol (2,5-ddol), which is then metabolized to 2,5-dichlorohydroquinone. here, we isolated from the genomic library of ut26 two genes which expressed 2,5-ddol dehydrogenase activity when they were transformed into p. putida and escherichia coli. both gene products had an apparent molecular size of 28 kda by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the fir ... | 1994 | 7515041 |
| bacteriophages from bombyx mori. | preparations of silkworm larvae contained two large phages with contractile tails (myoviridae). one phage was active on pseudomonas paucimobilis. the other, not cultivated, was one of the largest viruses known. | 1994 | 7979992 |
| detection of polychlorinated biphenyl degradation genes in polluted sediments by direct dna extraction and polymerase chain reaction. | it was the aim of this study to specifically detect the dna sequences for the bphc gene, the meta-cleavage enzyme of the aerobic catabolic pathway for biphenyl and polychlorinated biphenyl degradation, in aquatic sediments without prior cultivation of microorganisms by using extraction of total dna, pcr amplification of bphc sequences, and detection with specific gene probes. the direct dna extraction protocol used was modified to enhance lysis efficiency. crude extracts of dna were further puri ... | 1993 | 8285706 |
| development of field application vectors for bioremediation of soils contaminated with polychlorinated biphenyls. | field application vectors (favs), which are a combination of a selective substrate, a host, and a cloning vector, have been developed for the purpose of expressing foreign genes in nonsterile, competitive environments in which the gene products provide no advantage to the host. such gene products are exemplified by the enzymes for the cometabolism of polychlorinated biphenyls (pcbs) through the biphenyl degradation pathway. attempts to use highly competent pcb-cometabolizing strains in the envir ... | 1993 | 8328798 |
| cloning and sequence analysis of a hemolysin-encoding gene from pseudomonas paucimobilis. | we report the cloning, expression and nucleotide (nt) sequence of a beta-hemolysin-encoding gene, termed hlya, from pseudomonas paucimobilis. a genomic dna library of the pseudomonad was constructed in escherichia coli using the plasmid vector, puc19. the hlya gene was cloned by screening for a beta-hemolytic phenotype in e. coli transformants and was mapped to a 1100-bp psti-smai fragment. the nt sequence analysis of the 1100-bp insert revealed a 789-bp open reading frame which is preceded by a ... | 1993 | 8344528 |
| [pseudomonas paucimobilis infections. an analysis of 2 cases]. | 1993 | 8497747 | |
| classification of rhizomonas suberifaciens, an unnamed rhizomonas species, and sphingomonas spp. in rrna superfamily iv. | thermal melting profiles of hybrids between 3h-labeled rrna of rhizomonas suberifaciens, the causal agent of corky root of lettuce, and chromosomal dnas from 27 species of gram-negative bacteria indicated that the genus rhizomonas belongs to superfamily iv of de ley. on the basis of the melting temperatures of dna hybrids with rrnas from the type strains of r. suberifaciens, sphingomonas paucimobilis, and sphingomonas capsulata, rhizomonas strains constitute a separate branch in superfamily iv, ... | 1993 | 8427800 |
| isolation and characterization of tn5-induced mutants of pseudomonas paucimobilis ut26 defective in gamma-hexachlorocyclohexane dehydrochlorinase (lina). | pseudomonas paucimobilis ut26 grows on gamma-hexachlorocyclohexane (gamma-hch) as a sole source of carbon and energy. tn5 mutation was introduced into ut26, and two kinds of mutants defective in gamma-hch degradation were phenotypically isolated; one (ut64) completely lacked the activity to degrade gamma-hch, while the other (ut61) retained a very low level of activity. tagging and sequencing analysis showed that both mutants had a tn5 insertion at the same site of the lina (gamma-hch dehydrochl ... | 1993 | 7686793 |
| cloning and sequencing of a dehalogenase gene encoding an enzyme with hydrolase activity involved in the degradation of gamma-hexachlorocyclohexane in pseudomonas paucimobilis. | in pseudomonas paucimobilis ut26, gamma-hexachlorocyclohexane (gamma-hch) is converted by two steps of dehydrochlorination to a chemically unstable intermediate, 1,3,4,6-tetrachloro-1,4-cyclohexadiene (1,4-tcdn), which is then metabolized to 2,5-dichloro-2,5-cyclohexadiene-1,4-diol (2,5-ddol) by two steps of hydrolytic dehalogenation via the chemically unstable intermediate 2,4,5-trichloro-2,5-cyclohexadiene-1-ol (2,4,5-dnol). to clone a gene encoding the enzyme responsible for the conversion of ... | 1993 | 7691794 |
| cloning, expression, and sequence analysis of a lignostilbene-alpha,beta-dioxygenase gene from pseudomonas paucimobilis tmy1009. | from the genomic library of pseudomonas paucimobilis tmy1009 constructed with the cosmid phc79, an 8-kb bamhi-kpni fragment encoding lignostilbene-alpha,beta-dioxygenase (lsd) was cloned into puc19 (designated pksn2510). e. coli jm109 having pksn2510 produced a small amount of lsd only when the lac promoter was induced by isopropyl-beta-d-thio-galactopyranoside (iptg). the 1.9-kb sali fragment containing the lsd gene on pksn2510 was subcloned into puc119 in opposite directions (designated pkhn25 ... | 1993 | 7763879 |
| structural and enzymatical comparison of lignostilbene-alpha,beta-dioxygenase isozymes, i, ii, and iii, from pseudomonas paucimobilis tmy1009. | three isozymes of lignostilbene-alpha,beta-dioxygenase (lsd) from pseudomonas paucimobilis tmy1009 were separated on qae-toyopearl chromatography. all active fractions were further chromatographed on deae-toyopearl, butyl-toyopearl, and sephacryl s-300 columns. then the isozymes i, ii, and iii were purified homogeneously. all three isozymes consisted of two subunits with the same mol. mass. according to the n-terminal amino acid sequences up to 25 residues of these three isozymes and the reverse ... | 1993 | 7763880 |
| characterization of the c alpha-dehydrogenase gene involved in the cleavage of beta-aryl ether by pseudomonas paucimobilis. | c alpha-dehydrogenase catalyzes the oxidation of arylglycerol-beta-aryl ether at the c alpha-position, and therefore this process produces the specific substrate for beta-etherase, which cleaves beta-ary ethers (c alpha carbonyl type). here we isolated the c alpha-dehydrogenase gene (lig d) and sequenced its nucleotides. this gene contains an open reading frame of 915 bp and the deduced amino acid sequence had a homology with the ribitol dehydrogenase family. lig d is about 1 kbp upstream of the ... | 1993 | 7764263 |
| acanthamoeba keratitis: synergy between amebic and bacterial cocontaminants in contact lens care systems as a prelude to infection. | we encountered a patient with acanthamoeba keratitis whose contact lens care solution contained numerous trophozoites and cysts admixed with xanthomonas maltophilia organisms, many of which were adherent to the trophozoite surface and internalized within endocytic vacuoles. because of this finding, we investigated the role of bacterial cocontaminants in contact lens care systems as substrates for the growth of acanthamoeba spp. individual cocultivation of acanthamoeba castellanii and a. polyphag ... | 1992 | 1401013 |
| infections with pseudomonas paucimobilis. | 1992 | 1562669 | |
| septic shock caused by pseudomonas paucimobilis. | 1992 | 1562670 | |
| development and use of field application vectors to express nonadaptive foreign genes in competitive environments. | many potential applications of genetically engineered microorganisms in environmental and agricultural biotechnology involve introducing genetic capabilities into nonsterile competitive environments in which they provide no advantage to the host. field application vectors have been designed for the purpose of creating a temporary niche for the host in such environments. this technique involves the addition to the target environment of a selective substrate readily utilizable by the host microorg ... | 1992 | 16348652 |
| determinants encoding resistance to several heavy metals in newly isolated copper-resistant bacteria. | three copper-resistant, gram-negative bacteria were isolated and characterized. of the three strains, alcaligenes denitrificans ah tolerated the highest copper concentration (mic = 4 mm cuso(4)). all three strains showed various levels of resistance to other metal ions. a. denitrificans ah contains sequences which cross-hybridized with the mer (mercury resistance) determinant of tn21 and the czc (cobalt, zinc, and cadmium resistance), cnr (cobalt and nickel resistance), and chr (chromate resista ... | 1991 | 16348575 |
| characterization of a beta-lactamase produced by pseudomonas paucimobilis. | a novel beta-lactamase enzyme produced by a strain of pseudomonas paucimobilis is described. the enzyme differs from other recorded beta-lactamases from gram-negative aerobic bacteria. it was constitutive, and had the characteristics of a penicillinase. one single band of beta-lactamase activity at pi 4.6 was seen on iso-electric focusing. the enzyme had a molecular mass of 30 kda. the beta-lactamase was strongly inhibited by tazobactam, sulbactam and clavulanic acid but not by the thiol residue ... | 1991 | 1655952 |
| pseudomonas paucimobilis contamination in haemodialysis fluid. | 1991 | 1672328 | |
| infections with pseudomonas paucimobilis: report of four cases and review. | pseudomonas paucimobilis (formerly cdc group iik, biotype 1) is a strictly aerobic, nonfermenting, oxidase- and catalase-positive, gram-negative bacillus that is widely distributed in water and soil. its name derives from the difficulty encountered in demonstrating its motility, even in liquid media. this microorganism is responsible for two types of infection in humans: sporadic or community-acquired infections, probably of endogenous or environmental origin (bacteremia, meningitis, urinary tra ... | 1991 | 1775840 |
| metabolism of naphthalene by the biphenyl-degrading bacterium pseudomonas paucimobilis q1. | pseudomonas paucimobilis q1 originally isolated as biphenyl degrading organism (furukawa et al. 1983), was shown to grow with naphthalene. after growth with biphenyl or naphthalene the strain synthesized the same enzyme for the ring cleavage of 2,3-dihydroxybiphenyl or 1,2-dihydroxynaphthalene. the enzyme, although characterized as 2,3-dihydroxybiphenyl dioxygenase (taira et al. 1988), exhibited considerably higher relative activity with 1,2-dihydroxynaphthalene. these results demonstrate that t ... | 1991 | 1368152 |
| cloning of a lignostilbene-alpha,beta-dioxygenase gene from pseudomonas paucimobilis tmy1009. | 1991 | 1368688 | |
| molecular cloning of a pseudomonas paucimobilis gene encoding a 17-kilodalton polypeptide that eliminates hcl molecules from gamma-hexachlorocyclohexane. | pseudomonas paucimobilis ut26 is capable of growing on gamma-hexachlorocyclohexane (gamma-hch). a genomic library of p. paucimobilis ut26 was constructed in pseudomonas putida by using the broad-host-range cosmid vector pks13. after 2,300 clones were screened by gas chromatography, 3 clones showing gamma-hch degradation were detected. a 5-kb fragment from one of the cosmid clones was subcloned into puc118, and subsequent deletion and gas chromatography-mass spectrometry analyses revealed that a ... | 1991 | 1718942 |
| pseudomeningitis caused by pseudomonas paucimobilis. | 1991 | 2032866 | |
| chemical structure of glycosphingolipids isolated from sphingomonas paucimobilis. | two novel glycosphingolipids were isolated from sphingomonas paucimobilis and their structures were completely elucidated. the glycosyl portion of the glycosphingolipid consists of an alpha-d-manp-[1----2)-alpha-d-galp-(1----6)-alpha-d-glcpn-(1 ----4)-alpha-d- glcpa-r tetrasaccharide. the hydrophobic residue r was found to be heterogeneous with respect to the dihydrosphingosine residue. erythro-1,3-dihydroxy-2-amino-octadecane and erythro-1,3-dihydroxy-2-amino-cis-13,14-methyleneoctadecane were ... | 1991 | 1959589 |
| multiplication of legionella spp. in tap water containing hartmannella vermiformis. | a model was developed to study the multiplication of various legionella spp. in tap water containing hartmannella vermiformis. tap water cultures prepared with the following components were suitable for the multiplication studies: legionella spp., 10(3) cfu/ml; h. vermiformis, 10(4.4) cysts per ml; and killed pseudomonas paucimobilis, 10(9) cells per ml. cocultures were incubated at 37 degrees c for at least 1 week. the following legionellae multiplied in tap water cocultures in each replicate e ... | 1991 | 1892386 |
| cloning and sequencing of the gene for a pseudomonas paucimobilis enzyme that cleaves beta-aryl ether. | we isolated pseudomonas paucimobilis syk-6, which was able to degrade various dimeric lignin compounds (y. katayama, s. nishikawa, m. nakamura, k. yano, m. yamasaki, n. morohoshi, and t. haraguchi, mokuzai gakkaishi 33:77-79, 1987). this metabolic process is a distinct characteristic of this bacterium, which is equipped with an enzymatic modification system for various dimeric lignin compounds involved in the tricarboxylic acid cycle. cleavage of the beta-aryl ether linkage is essential in this ... | 1991 | 1744051 |
| contamination during in vitro processing of bone marrow for transplantation: clinical significance. | we determined the prevalence and clinical significance of positive microbiologic cultures obtained from 194 consecutive bone marrow harvests intended for infusion in 188 consecutive adult bone marrow transplant patients. microbiologic cultures were obtained at harvest, after manipulation in vitro (for abo imcompatibility or purging procedures), and at the time of thawing and infusion (after earlier cryopreservation). only one of 194 marrow harvests was culture-positive intra-operatively (from an ... | 1991 | 2059759 |
| purification and characterization of a 1,2-dihydroxynaphthalene dioxygenase from a bacterium that degrades naphthalenesulfonic acids. | 1,2-dihydroxynaphthalene dioxygenase was purified to homogeneity from a bacterium that degrades naphthalenesulfonic acids (strain bn6). the enzyme requires fe2+ for maximal activity and consists of eight identical subunits with a molecular weight of about 33,000. analysis of the nh2-terminal amino acid sequence revealed a high degree of homology (22 of 29 amino acids) with the nh2-terminal amino acid sequence of 2,3-dihydroxybiphenyl dioxygenase from strain pseudomonas paucimobilis q1. 1,2-dihyd ... | 1991 | 2050635 |
| isolation and characterization of a fluoranthene-utilizing strain of pseudomonas paucimobilis. | a soil bacterium capable of utilizing fluoranthene as the sole source of carbon and energy for growth was purified from a seven-member bacterial community previously isolated from a creosote waste site for its ability to degrade polycyclic aromatic hydrocarbons. by standard bacteriological methods, this bacterium was characterized taxonomically as a strain of pseudomonas paucimobilis and was designated strain epa505. utilization of fluoranthene by strain epa 505 was demonstrated by increase in b ... | 1990 | 2339870 |
| proposals of sphingomonas paucimobilis gen. nov. and comb. nov., sphingomonas parapaucimobilis sp. nov., sphingomonas yanoikuyae sp. nov., sphingomonas adhaesiva sp. nov., sphingomonas capsulata comb. nov., and two genospecies of the genus sphingomonas. | based on the partial nucleotide sequence analysis of 16s ribosomal ribonucleic acid (rrna), presence of unique sphingoglycolipids in cellular lipid, and the major type of ubiquinone (q10), we propose sphingomonas gen. nov. with the type species sphingomonas paucimobilis (holmes et al, 1977) comb. nov. from the homology values of deoxyribonucleic acid-deoxyribonucleic acid hybridization and the phenotypic characteristics, three new species, sphingomonas parapaucimobilis, sphingomonas yanoikuyae, ... | 1990 | 2111872 |
| pseudomonas paucimobilis bacteraemia associated with haemodialysis. | we report a case of pseudomonas paucimobilis bacteraemia where the source was presumed to be related to haemodialysis. previous reports have identified this organism as a pathogen in a variety of disease states including bacteraemia of unknown origin and in patients undergoing peritoneal dialysis. | 1990 | 1972955 |
| degradation of phenanthrene, fluorene and fluoranthene by pure bacterial cultures. | bacterial mixed cultures able to degrade the polycyclic aromatic hydrocarbons (pah) phenanthrene, fluorene and fluoranthene, were obtained from soil using conventional enrichment techniques. from these mixed cultures three pure strains were isolated: pseudomonas paucimobilis degrading phenanthrene; p. vesicularis degrading fluorene and alcaligenes denitrificans degrading fluoranthene. the maximum rates of pah degradation ranged from 1.0 mg phenanthrene/ml per day to 0.3 mg fluoranthene/ml per da ... | 1990 | 1366395 |
| phosphonate utilization by bacteria in the presence of alternative phosphorus sources. | batch and continuous culture experiments were carried out to investigate the effect of orthophosphate and p-nitrophenylphosphate on the utilization of various phosphonates as a p source by bacteria. detailed tests with methylphosphonate as a model phosphonate and the phosphonate-degrading pseudomonas paucimobilis strain mmm101a revealed that, in contrast with the majority of literature data, the phosphates did not suppress phosphonate utilization. under conditions of p stress, strain mmm101a sim ... | 1990 | 1368141 |
| chemical structure and biological activity of lipooligosaccharide isolated from sphingomonas paucimobilis, a gram-negative bacterium lacking usual lipopolysaccharide. | 1990 | 2101144 | |
| molecular cloning of the protocatechuate 4,5-dioxygenase genes of pseudomonas paucimobilis. | we determined the nucleotide sequence of a 1.9-kilobase fragment of pseudomonas paucimobilis syk6 chromosomal dna that included genes encoding protocatechuate 4,5-dioxygenase, the enzyme responsible for the aromatic ring fission of protocatechuate. two open reading frames of 417 and 906 base pairs were found that had no homology with previously reported sequences, including those encoding protocatechuate 3,4-dioxygenase. since both open reading frames were indispensable for the enzyme activity, ... | 1990 | 2185230 |
| pseudomonas paucimobilis peritonitis in a patient on capd successfully treated with ciprofloxacin and netilmicin. | 1990 | 2209631 |