Publications
Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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comparison of directigen flu a+b with real time pcr in the diagnosis of influenza. | early diagnosis and treatment of patients with influenza is the reason why physicians need rapid high-sensitivity influenza diagnostic tests that require no complex lab equipment and can be performed and interpreted within 15 min. the aim of this study was to compare the rapid directigen flu a+b test with real time pcr for detection of influenza viruses in the republic of macedonia. | 2016 | 26933779 |
robust lys63-linked ubiquitination of rig-i promotes cytokine eruption in early influenza b virus infection. | influenza a and b virus infections both cause a host innate immunity response. here, we report that the robust production of type i and iii interferons (ifns), ifn-stimulated genes, and proinflammatory factors can be induced by influenza b virus rather than influenza a virus infection in alveolar epithelial (a549) cells during early infection. this response is mainly dependent on the retinoic acid-inducible gene i (rig-i)-mediated signaling pathway. infection by influenza b virus promotes intens ... | 2016 | 27122586 |
evidence update: glaxosmithkline's inactivated quadrivalent influenza vaccines. | inactivated trivalent influenza vaccines (iiv3s) are designed to protect against illness caused by two influenza a virus subtypes and one influenza b virus lineage. they may provide inadequate protection due to the co-circulation of viruses from two antigenically distinct influenza b lineages. incorporating strains from both b lineages as in inactivated quadrivalent influenza vaccines (iiv4s) reduces this risk. we summarize the evidence supporting two iiv4s manufactured by gsk vaccines. compared ... | 2016 | 26641539 |
identification of pyrrolo[3,2-c]pyridin-4-amine compounds as a new class of entry inhibitors against influenza viruses in vitro. | various influenza virus entry inhibitors are being developed as therapeutic antiviral agents in ongoing preparation for emerging influenza viruses, particularly those that may possess drug resistance to the current fda-approved neuraminidase inhibitors. in this study, small molecules having the pyrrolopyridinamine (ppa), aminothiadiazole (atd), dihydrofuropyridine carboxamide (hpc), or imidazopyridinamine (ipa) moiety were selected from a target-focused chemical library for their inhibitory acti ... | 2016 | 27586275 |
geolocalization of influenza outbreak within an acute care population: a layered-surveillance approach. | we seek to use a novel layered-surveillance approach to localize influenza clusters within an acute care population. the first layer of this system is a syndromic surveillance screen to guide rapid polymerase chain reaction testing. the second layer is geolocalization and cluster analysis of these patients. we posit that any identified clusters could represent at-risk populations who could serve as high-yield targets for preventive medical interventions. | 2016 | 27772674 |
disruption of src homology 3-binding motif within non-structural protein 1 of influenza b virus unexpectedly enhances viral replication in human cells. | the influenza virus non-structural protein 1 (ns1) is a multifunctional virulence factor that plays a crucial role during infection by blocking the innate antiviral immune response of infected cells. in contrast to the well-studied ns1 protein of influenza a virus, knowledge about structure and functions of the influenza b virus homologue b/ns1, which shares less than 25 % sequence identity, is still limited. here, we report on a reverse genetic analysis to study the role of a highly conserved c ... | 2016 | 27654951 |
analytical performance of the bd veritor™ system for rapid detection of influenza virus a and b in a primary healthcare setting. | infections with influenza a virus cannot be clinically differentiated from infections caused by influenza b virus or other respiratory viruses. additionally, although antiviral treatment is available for influenza a virus, it is not effective for the other viruses and must be initiated early in the course of disease for it to be effective. for these reasons, there is a need for a rapid, accurate diagnostic test for use in physicians' offices at the time patients are seen. we report the first fie ... | 2016 | 27612949 |
direct comparison of alere i and cobas liat influenza a and b tests for rapid detection of influenza virus infection. | we compared two rapid, point-of care nucleic acid amplification tests for detection of influenza a and b viruses (alere i [alere] and cobas liat [roche diagnostics]) with the influenza a and b virus test components of the filmarray respiratory panel (biofire diagnostics) using 129 respiratory specimens collected in universal viral transport medium (80 influenza a virus and 16 influenza b virus positive) from both adult and pediatric patients. the sensitivities of the alere test were 71.3% for in ... | 2016 | 27582513 |
epidemiological and virological characterization of influenza b virus infections. | while influenza a viruses comprise a heterogeneous group of clinically relevant influenza viruses, influenza b viruses form a more homogeneous cluster, divided mainly into two lineages: victoria and yamagata. this divergence has complicated seasonal influenza vaccine design, which traditionally contained two seasonal influenza a virus strains and one influenza b virus strain. we examined the distribution of the two influenza b virus lineages in israel, between 2011-2014, in hospitalized and in n ... | 2016 | 27533045 |
recombinant production and characterization of human anti-influenza virus monoclonal antibodies identified from hybridomas fused with human lymphocytes. | in previous studies, hybridomas producing human immunoglobulin g, the antibodies 5e4 and 5a7 against influenza a and b virus were established using a novel human lymphocyte fusion partner, spymeg. in the present study, we succeeded in achieving the recombinant production and secretion of 5e4 and 5a7 in chinese hamster ovary cells. our n-glycan analysis by intact-mass detection and liquid chromatography mass spectrometry showed that recombinant 5e4 and 5a7 have one n-glycan and the typical mammal ... | 2016 | 27464991 |
development of high-yield influenza b virus vaccine viruses. | the burden of human infections with influenza a and b viruses is substantial, and the impact of influenza b virus infections can exceed that of influenza a virus infections in some seasons. over the past few decades, viruses of two influenza b virus lineages (victoria and yamagata) have circulated in humans, and both lineages are now represented in influenza vaccines, as recommended by the world health organization. influenza b virus vaccines for humans have been available for more than half a c ... | 2016 | 27930325 |
multiplex assay for subtyping avian influenza a viruses by cdna hybridization and adapter-mediated amplification. | multiple subtypes of influenza a viruses circulating in animals must be closely monitored to understand their risk to humans and animal populations. many molecular-based subtyping methods require constant monitoring of viral genomes for primer and/or probe mismatches and are prone to primer-primer interactions. this report presents a new approach that involves target enrichment through cdna hybridization followed by adapter-mediated amplification for subtyping influenza virus (amasiv). as a proo ... | 2016 | 27372075 |
long-term detection of seasonal influenza rna in faeces and intestine. | some cases of seasonal influenza virus (human influenza a virus (iav)/human influenza b virus (ibv)) are associated with abdominal symptoms. although virus rna has been detected in faeces, intestinal infection has not been clearly demonstrated. we aimed to provide evidence that iav/ibv infects the human intestine. this prospective observational study measured virus rna in faecal and sputum samples from 22 patients infected with iav/ibv (19 iav positive and three ibv positive). nineteen patients ... | 2016 | 27424942 |
acute liver failure: outcome and value of pediatric end-stage liver disease score in pediatric cases. | the aims of this study were to analyze the characteristics of patients with acute liver failure (alf) in our center and evaluate the prognostic value of the pediatric end-stage liver disease (peld) score calculated at admission. | 2016 | 27749626 |
tmprss2 independency for haemagglutinin cleavage in vivo differentiates influenza b virus from influenza a virus. | influenza a and b viruses show clear differences in their host specificity and pandemic potential. recent studies have revealed that the host protease tmprss2 plays an essential role for proteolytic activation of h1, h3, and h7 subtype strains of influenza a virus (iav) in vivo. iav possessing a monobasic cleavage site in the haemagglutinin (ha) protein replicates poorly in tmprss2 knockout mice owing to insufficient ha cleavage. in the present study, human isolates of influenza b virus (ibv) st ... | 2016 | 27389476 |
replication-competent fluorescent-expressing influenza b virus. | influenza b viruses (ibvs) cause annual outbreaks of respiratory illness in humans and are increasingly recognized as a major cause of influenza-associated morbidity and mortality. studying influenza viruses requires the use of secondary methodologies to identify virus-infected cells. to this end, replication-competent influenza a viruses (iavs) expressing easily traceable fluorescent proteins have been recently developed. in contrast, similar approaches for ibv are mostly lacking. in this repor ... | 2016 | 26590325 |
performance of a novel point-of-care molecular assay for detection of influenza a and b viruses and respiratory syncytial virus (enigma minilab) in children with acute respiratory infection. | the performance of the enigma minilab assay for influenza a and b viruses and respiratory syncytial virus (rsv) was compared to a centralized laboratory respiratory virus panel. the positive and negative percent agreement for influenza a virus, influenza b virus, and rsv were 79.2% (95% confidence interval [95% ci], 57.8 to 92.9%) and 99.4% (95% ci, 98.4 to 99.9), 100% (95% ci, 47.8 to 100%) and 100% (95% ci, 99.3 to 100%), 98.5% (95% ci, 94.6 to 99.8%) and 94.5% (95% ci, 91.9 to 96.4%), respect ... | 2016 | 26560540 |
[visual detection of human coronavirus nl63 by reverse transcription loop-mediated isothermal amplification]. | a simple and sensitive assay for rapid detection of human coronavirus nl63 (hcov-nl63) was developed by colorimetic reverse transcription loop-mediated isothermal amplification (rt-lamp). the method employed six specially designed primers that recognized eight distinct regions of the hcov-nl63 nucleocapsid protein gene for amplification of target sequences under isothermal conditions at 63 degrees c for 1 h amplification of rt-lamp was monitored by addition of calcein before amplification. a pos ... | 2016 | 27295884 |
[evaluation of the efficiency of differential diagnosis of influenza by multiplex real-time polymerase chain reaction]. | the paper gives the results of a comparative analysis of the detection of influenza viruses in clinical samples, by using multiplex real-time polymerase chain reaction (rt-pcr) and by virus isolation in mdck cell cultures. the investigation employed 267 nasopharyngeal swab specimens obtained from patients with influenza symptoms during two epidemic seasons (2008-2009 and 2009-2010). influenza viruses were found in 104 samples (48 with influenza a virus (iav) and 56 with influenza b virus (ibv)) ... | 2016 | 22624473 |
an investigation into respiratory tract viruses in children with acute lower respiratory tract infection or wheezing. | this study aimed to determine the frequencies of respiratory tract viruses in patient (acute lower respiratory tract infection (lrti) or wheezing) and control (history of asthma without symptoms) groups. | 2016 | 27854115 |
[detection of respiratory viruses in children with acute lower respiratory tract infection: an analysis of 5,150 children]. | to investigate the distribution of respiratory viruses on throat swabs in hospitalized children with acute lower respiratory tract infection (alrti). | 2016 | 26781413 |
spirometry filters can be used to detect exhaled respiratory viruses. | respiratory viruses are very common in the community and contribute to the burden of illness for patients with chronic respiratory diseases, including acute exacerbations. traditional sampling methods are invasive and problematic to repeat. accordingly, we explored whether respiratory viruses could be isolated from disposable spirometry filters and whether detection of viruses in this context represented presence in the upper or lower respiratory tract. discovery (n = 53) and validation (n = ... | 2016 | 27669334 |
spatial and temporal spread of acute viral respiratory infections in young children living in high-altitude rural communities: a prospective household-based study. | few studies have described patterns of transmission of viral acute respiratory infections (ari) in children in developing countries. we examined the spatial and temporal spread of viral ari among young children in rural peruvian highland communities. previous studies have described intense social interactions in those communities, which could influence the transmission of viral infections. | 2016 | 27404599 |
ineffectiveness of the 2014-2015 h3n2 influenza vaccine. | the seasonal influenza vaccine is currently the most effective preventive modality against influenza infection. nasopharyngeal samples of vaccinated and non-vaccinated patients presenting with influenza-like-illness (ili) were collected from over 20 outpatient clinics located in different geographic parts of israel and were tested for the presence of influenza viruses (influenza a and influenza b). here we show, that in the 2014-2015 season, the vaccine that included the a/texas/50/2012 h3n2 vir ... | 2016 | 26716420 |
viral etiology of medically attended influenza-like illnesses in children less than five years old in suzhou, china, 2011-2014. | limited information is available on the non-influenza etiology and epidemiology of influenza-like illness (ili) in china. from april 2011 to march 2014, we collected oropharyngeal swabs from children less than 5 years of age with symptoms of ili who presented to the outpatient departments of suzhou university affiliated children's hospital (sch). we used reverse transcription polymerase chain reaction (rt-pcr) or pcr to detect 11 respiratory viruses. among 3,662 enrolled ili patients, 1,292 (35. ... | 2016 | 26792409 |
prevalence of human metapneumovirus infection among patients with influenza-like illness: report from a tertiary care centre, southern india. | human metapneumovirus (hmpv), discovered in the 21st century, has emerged as an important cause of influenza-like illness in children and adults causing mild upper respiratory tract infection to severe bronchiolitis and community-associated pneumonia. the aim of this study was to determine the prevalence of hmpv in the union territory of puducherry, india, as part of national influenza surveillance programme. | 2016 | 26776115 |
exposure to common respiratory bacteria alters the airway epithelial response to subsequent viral infection. | colonization of the airways with potential pathogenic bacteria is observed in a number of chronic respiratory diseases, such as copd or cystic fibrosis. infections with respiratory viruses are known triggers of exacerbations of these diseases. we here investigated if pre-exposure to bacteria alters the response of lung epithelial cells to subsequent viral infection. | 2016 | 27259950 |
waning protection of influenza vaccine against mild laboratory confirmed influenza a(h3n2) and b in spain, season 2014-15. | the 2014/15 influenza season in spain was dominated by the circulation of drifted a(h3n2) and co-circulation of b viruses. we present the final estimates of influenza vaccine effectiveness (ive) against confirmed influenza a(h3n2) and b its evolution along the season and with time since vaccination. | 2016 | 27020713 |
influenza virus shedding in laninamivir-treated children upon returning to school. | the current school health and safety act in japan states that children with influenza infection should stay home until day 6(th) after symptoms onset. this was an amendment of a previous version recommending school return on day 3 after defervescence. here, we investigated the duration of fever and virus shedding after laninamivir treatment in 7 children infected with influenza a(h3n2) virus and 21 children with influenza b virus in relation to the school return timing recommended by the school ... | 2016 | 26806610 |
phenotypic and genotypic analysis of influenza viruses isolated from adult subjects during a phase ii study of intravenous zanamivir in hospitalised subjects. | intravenous zanamivir (ivz) is a neuraminidase (na) inhibitor (nai) under investigation for the treatment of subjects hospitalised with influenza. the study included 130 symptomatic, hospitalised adults with influenza. subjects received ivz for 5-10 days. viruses were cultured and analysed for susceptibility to zanamivir. mean ic50s (n = 50) (±sd) for influenza a/h1n1pdm09, a/h3n2 and influenza b were 0.20 ± 0.06, 0.26 ± 0.07 and 1.61 ± 0.35 nm, respectively, and are comparable to data observed ... | 2016 | 27568923 |
[prevalence characteristics of influenza viruses in children in beijing during 2014-2015 and 2015-2016 influenza seasons]. | to compare the prevalence characteristics of influenza viruses in children in beijing during 2014-2015 and 2015-2016 influenza seasons. | 2016 | 27510869 |
incidence and clinical course of respiratory viral coinfections in children aged 0-59 months. | clinical data available on coinfections are contradictory concerning both the number of viruses involved and the severity of the condition. a total of 114 patients aged 0-59 months with symptoms of respiratory tract infection were enrolled into the study. nasal and pharyngeal swabs were tested using the pcr method for the following 12 viruses: influenza a, influenza b, respiratory syncytial virus a (rsv a), respiratory syncytial virus b (rsv b), adenovirus, metapneumovirus, coronavirus 229e/nl63 ... | 2016 | 26801151 |
antibiotic prescription practices among children with influenza. | the important factor in the development of resistance to antibiotics is their overuse, especially for viral respiratory infections. the aim of the study was to find out the frequency of the antibiotic therapy administrated to children with influenza. a total of 114 children younger than 59 months seeking care for the acute respiratory tract infection was enrolled into the study. the patients had influenza-like symptoms: fever > 38 °c, cough, and sore throat of less than 4 days duration. nasal an ... | 2016 | 26801146 |
incidence of medically attended influenza among residents of shai-osudoku and ningo-prampram districts, ghana, may 2013 - april 2015. | influenza vaccination is recommended by the world health organization for high risk groups, yet few data exist on influenza disease burden in west africa. | 2016 | 27964716 |
in vitro neuraminidase inhibitory concentration (ic50) of four neuraminidase inhibitors against clinical isolates of the influenza viruses circulating in the 2010-2011 to 2014-2015 japanese influenza seasons. | to assess the extent of viral resistance to the four neuraminidase inhibitors (nais), we measured their 50% inhibitory concentration (ic50) for influenza virus isolates from the 2014-2015 influenza season for comparison with those circulating in the 2010-2011 to 2013-2014 influenza seasons. viral isolation was done with specimens obtained prior to treatment, and the type and subtype of influenza was determined by rt-pcr using type- and subtype-specific primers. the ic50 was determined by a neura ... | 2016 | 27346379 |
sialic acid content in human saliva and anti-influenza activity against human and avian influenza viruses. | it was shown previously that human saliva has higher antiviral activity against human influenza viruses than against h5n1 highly pathogenic avian influenza viruses, and that the major anti-influenza activity was associated with sialic-acid-containing molecules. to further characterize the differential susceptibility to saliva among influenza viruses, seasonal influenza a and b virus, pandemic h1n1 virus, and 15 subtypes of avian influenza virus were tested for their susceptibility to human and c ... | 2016 | 26671828 |
susceptibility of influenza viruses circulating in western saudi arabia to neuraminidase inhibitors. | to investigate the sensitivity of circulating influenza viruses in western saudi arabia to neuraminidase inhibitors (nais); mainly, zanamivir and oseltamivir. | 2016 | 27052292 |
trivalent inactivated influenza vaccine effective against influenza a(h3n2) variant viruses in children during the 2014/15 season, japan. | the 2014/15 influenza season in japan was characterised by predominant influenza a(h3n2) activity; 99% of influenza a viruses detected were a(h3n2). subclade 3c.2a viruses were the major epidemic a(h3n2) viruses, and were genetically distinct from a/new york/39/2012(h3n2) of 2014/15 vaccine strain in japan, which was classified as clade 3c.1. we assessed vaccine effectiveness (ve) of inactivated influenza vaccine (iiv) in children aged 6 months to 15 years by test-negative case-control design ba ... | 2016 | 27784529 |
2014-2015 influenza vaccine effectiveness in the united states by vaccine type. | circulating a/h3n2 influenza viruses drifted significantly after strain selection for the 2014-2015 vaccines. also in 2014-2015, the advisory committee on immunization practices recommended preferential use of live attenuated influenza vaccine (laiv) over inactivated influenza vaccine (iiv) among children aged 2-8 years. | 2016 | 27702768 |
surveillance for severe acute respiratory infections in southern arizona, 2010-2014. | the binational border infectious disease surveillance program began surveillance for severe acute respiratory infections (sari) on the us-mexico border in 2009. here, we describe patients in southern arizona. | 2016 | 26590069 |
[differential diagnosis of the viral etiology of suspected mumps in a population with high vaccine coverage]. | in this study, buccal swabs from patients with the clinical picture of parotitis epidemica in whom mumps virus (mv) infection was not confirmed by direct detection or serologically were tested. the aim was to detect by molecular methods nucleic acids (nas) of other respiratory viruses possibly involved in salivary gland swelling. at the same time, paired sera, if available, were tested. | 2016 | 28078898 |
[benign acute childhood myositis: clinical series and literature review]. | benign acute childhood myositis (bacm) is a rare clinical condition that mainly affects pre-school and school age-children. it is usually preceded by a viral illness, particularly influenza virus infection. | 2016 | 27542581 |
prediction of influenza b vaccine effectiveness from sequence data. | influenza is a contagious respiratory illness that causes significant human morbidity and mortality, affecting 5-15% of the population in a typical epidemic season. human influenza epidemics are caused by types a and b, with roughly 25% of human cases due to influenza b. influenza b is a single-stranded rna virus with a high mutation rate, and both prior immune history and vaccination put significant pressure on the virus to evolve. due to the high rate of viral evolution, the influenza b vaccin ... | 2016 | 27473305 |
competitive fitness of influenza b viruses possessing e119a and h274y neuraminidase inhibitor resistance-associated substitutions in ferrets. | neuraminidase (na) inhibitors (nais) are the only antiviral drugs recommended for influenza treatment and prophylaxis. although nai-resistant influenza b viruses that could pose a threat to public health have been reported in the field, their fitness is poorly understood. we evaluated in ferrets the pathogenicity and relative fitness of reverse genetics (rg)-generated influenza b/yamanashi/166/1998-like viruses containing e119a or h274y na substitutions (n2 numbering). ferrets inoculated with na ... | 2016 | 27466813 |
humoral response to influenza vaccination in relation to pre-vaccination antibody titres, vaccination history, cytomegalovirus serostatus and cd4/cd8 ratio. | annual vaccination against influenza virus is generally recommended to elderly and chronically ill, but the relative importance of factors influencing the outcome is not fully understood. | 2016 | 27030916 |
public health and economic impact of seasonal influenza vaccination with quadrivalent influenza vaccines compared to trivalent influenza vaccines in europe. | influenza b strains represent on average 23% of all circulating strains in europe and when there is a vaccine mismatch on b strains, additional influenza-related hospitalizations and deaths as well as substantial additional costs are observed. the objective was to estimate the public health and economic impact of seasonal influenza vaccination with quadrivalent influenza vaccines (qiv) compared to trivalent influenza vaccines (tiv) in europe (eu). based on data from 5 eu countries (france, germa ... | 2016 | 27166916 |
[development of the quadrivalent live attenuated influenza vaccine including two influenza b lineages--victoria and yamagata]. | this work is devoted to the research of the live attenuated influenza vaccine (laiv) comprising two reassortant b/ussr/60/69-based vaccine influenza viruses victoria and yamagata. the intranasal immunization of the cba mice with both victoria and yamagata strains induced 100% lung protection against the subsequent infection with the wild-type influenza b viruses of any antigen lineage. the quadrivalent laiv (qlaiv) comprising both reassortant influenza b viruses victoria and yamagata were safe a ... | 2016 | 27145595 |
cost-effectiveness of quadrivalent versus trivalent influenza vaccine in the united states. | designed to overcome influenza b mismatch, new quadrivalent influenza vaccines (qivs) contain one additional b strain compared with trivalent influenza vaccines (tivs). | 2016 | 27987647 |
parameter scaling for epidemic size in a spatial epidemic model with mobile individuals. | in recent years, serious infectious diseases tend to transcend national borders and widely spread in a global scale. the incidence and prevalence of epidemics are highly influenced not only by pathogen-dependent disease characteristics such as the force of infection, the latent period, and the infectious period, but also by human mobility and contact patterns. however, the effect of heterogeneous mobility of individuals on epidemic outcomes is not fully understood. here, we aim to elucidate how ... | 2016 | 27973605 |
available influenza vaccines: immunization strategies, history and new tools for fighting the disease. | the main public health strategy for containing influenza-related disease is annual vaccination, which is recommended for the elderly and others belonging to risk-factor categories, who present the highest morbidity and mortality, as reported by the world health organization (who) recommendations. the availability of different influenza vaccine formulations makes the choice of the best immunization strategy a challenge for stakeholders and public health experts. heterogeneity in at-risk categorie ... | 2016 | 27346939 |
immunogenicity and safety of a cell culture-based quadrivalent influenza vaccine in adults: a phase iii, double-blind, multicenter, randomized, non-inferiority study. | quadrivalent influenza vaccines (qivs), which include both b lineage strains, are expected to provide broader protection than trivalent influenza vaccines (tivs). the non-inferiority, immunogenicity, and safety of a cell culture-based investigational qivc and 2 tivs (tiv1c, tiv2c), in adults (≥18 y), were evaluated in this phase iii, double-blind, multicenter study. a total of 2680 age-stratified subjects were randomized (2:1:1) to receive 1 dose of qivc (n = 1335), tiv1c (n = 676), or tiv2c (n ... | 2016 | 27322354 |
the clinical impact and cost effectiveness of quadrivalent versus trivalent influenza vaccination in finland. | trivalent influenza vaccines encompass one influenza b lineage; however, predictions have been unreliable on which of two antigenically distinct circulating lineages will dominate. quadrivalent seasonal influenza vaccines contain strains from both lineages. this analysis assesses the cost effectiveness of switching from trivalent inactivated influenza vaccination (tiv) in finland to quadrivalent vaccination, using inactivated (qiv) or live-attenuated (q-laiv) vaccines. | 2016 | 27423657 |
flutracking weekly online community survey of influenza-like illness annual report, 2015. | flutracking is a national online community influenza-like illness (ili) surveillance system that monitors weekly ili activity and impact in the australian community. this article reports on the 2015 findings from flutracking. from 2014 to 2015 there was a 38.5% increase in participants to 27,824 completing at least 1 survey with a peak weekly response of 25,071 participants. the 2015 flutracking national ili weekly fever and cough percentages peaked in late august at 5.0% in the unvaccinated gro ... | 2016 | 28043226 |
estimating the lineage dynamics of human influenza b viruses. | the prediction of the lineage dynamics of influenza b viruses for the next season is one of the largest obstacles for constructing an appropriate influenza trivalent vaccine. seasonal fluctuation of transmissibility and epidemiological interference between the two major influenza b lineages make the lineage dynamics complicated. here we construct a parsimonious model describing the lineage dynamics while taking into account seasonal fluctuation of transmissibility and epidemiological interferenc ... | 2016 | 27829058 |
influenza epidemiology in patients admitted to sentinel australian hospitals in 2015: the influenza complications alert network. | the influenza complications alert network (flucan) is a sentinel hospital-based surveillance program that operates at sites in all states and territories in australia. this report summarises the epidemiology of hospitalisations with laboratory-confirmed influenza during the 2015 influenza season. in this observational study, cases were defined as patients admitted to one of the sentinel hospitals with an acute respiratory illness with influenza confirmed by nucleic acid detection. during the per ... | 2016 | 28043227 |
comparison of two new generation influenza rapid diagnostic tests with instrument-based digital readout systems for influenza virus detection. | influenza rapid diagnostic tests (rdts) have been developed to supply scientists with more sensitive and specific techniques. newly developed digital reader-based techniques require test evaluations before their clinical application. | 2016 | 27327199 |
detection of influenza by real time rt-pcr is not affected by delays in respiratory specimen processing. | sample processing for real-time reverse transcriptase polymerase chain reaction (rrt-pcr) based diagnostic assays requires stabilizing sample ribonucleic acid (rna) in lysis buffer prior to testing. the stability of viral rna prior to processing is difficult to assure. it is unknown whether clinical sample integrity is compromised by delays in processing, as may occur due to weekends and holidays. we sought to examine the integrity of respiratory specimens with variable processing times. upper r ... | 2016 | 27089468 |
molecular basis of mrna cap recognition by influenza b polymerase pb2 subunit. | influenza virus polymerase catalyzes the transcription of viral mrnas by a process known as "cap-snatching," where the 5'-cap of cellular pre-mrna is recognized by the pb2 subunit and cleaved 10-13 nucleotides downstream of the cap by the endonuclease pa subunit. although this mechanism is common to both influenza a (flua) and influenza b (flub) viruses, flub pb2 recognizes a wider range of cap structures including m(7)gpppgm-, m(7)gpppg-, and gpppg-rna, whereas flua pb2 utilizes methylated g-ca ... | 2016 | 26559973 |
factors associated with real-time rt-pcr cycle threshold values among medically attended influenza episodes. | we evaluated the cycle threshold (ct) values of 1,160 influenza a positive and 806 influenza b positive specimens from two seasons of the us flu ve network to identify factors associated with ct values. low ct values (high genomic load) were associated with shorter intervals between illness onset and specimen collection, young age (ages 3-8 years old), and self-rated illness severity for both influenza a and b. low ct values were also associated with reported fever/feverishness and age ≥65 years ... | 2016 | 26334765 |
effect of risk factors on the prevalence of influenza infections among children of slums of dhaka city. | influenza viruses may cause severe acute respiratory illness among human population. people of densely populated areas, e.g., slum, are mostly affected by influenza viruses. although potential vaccines to influenza viruses have been developed, infection rate is still high, therefore, increase the morbidity and mortality rate in slum areas. to treat these infections, slum dwellers including children and mothers do not get proper medication as well as vaccination. hence, prevention remains to be t ... | 2016 | 27247898 |
comparative epidemiology of influenza a and b viral infection in a subtropical region: a 7-year surveillance in okinawa, japan. | the epidemic patterns of influenza b infection and their association with climate conditions are not well understood. influenza surveillance in okinawa is important for clarifying transmission patterns in both temperate and tropical regions. using surveillance data, collected over 7 years in the subtropical region of japan, this study aims to characterize the epidemic patterns of influenza b infection and its association with ambient temperature and relative humidity, in a parallel comparison wi ... | 2016 | 27821090 |
temporal patterns of influenza a and b in tropical and temperate countries: what are the lessons for influenza vaccination? | determining the optimal time to vaccinate is important for influenza vaccination programmes. here, we assessed the temporal characteristics of influenza epidemics in the northern and southern hemispheres and in the tropics, and discuss their implications for vaccination programmes. | 2016 | 27031105 |
a prospective study to assess the diagnostic performance of the sofia(®) immunoassay for influenza and rsv detection. | respiratory viruses rsv and influenza a and b viruses are responsible for important disease outbreaks during the winter season in temperate climate regions. rapid diagnostic tests (rdts) are assays designed to yield a rapid diagnosis, which facilitates patient management. the sofia influenza a+b fluorescence immunoassay and sofia rsv fluorescence immunoassay are rdts for influenza and rsv detection that employ a new technology to enhance their sensitivity. | 2016 | 26872325 |
changes of immunogenic profiles between a single dose and one booster influenza vaccination in hemodialysis patients - an 18-week, open-label trial. | annual influenza vaccination is recommended, but its efficacy in dialysis population is still controversial. here we aimed to compare the dynamic changes of immune response between various influenza vaccination protocols in hemodialysis patients. a 18-week open label, non-randomized, controlled trial was conducted during 2011-2012. the efficacy between unvaccinated, one- and two-dose regimens were evaluated in 175 hemodialysis patients. immunogenic profiles were assessed by hemagglutination-inhi ... | 2016 | 26869526 |
trivalent and quadrivalent influenza vaccination effectiveness in australia and south africa: results from a modelling study. | a modelling study was conducted to determine the effectiveness of trivalent (tiv) and quadrivalent (qiv) vaccination in south africa and australia. | 2016 | 26663701 |
epidemiological features of influenza in canadian adult intensive care unit patients. | to identify predictive factors and mortality of patients with influenza admitted to intensive care units (icu) we carried out a prospective cohort study of patients hospitalized with laboratory-confirmed influenza in adult icus in a network of canadian hospitals between 2006 and 2012. there were 626 influenza-positive patients admitted to icus over the six influenza seasons, representing 17·9% of hospitalized influenza patients, 3·1/10,000 hospital admissions. variability occurred in admission r ... | 2016 | 26384310 |
differential age susceptibility to influenza b/victoria lineage viruses in the 2015 australian influenza season. | influenza b viruses make up an important part of the burden from seasonal influenza globally. the 2015 season in australia saw an unusual predominance of influenza b with a distinctive switch during the season from b/yamagata/16/88 lineage viruses to b/victoria/2/87 lineage viruses. we also noted significant differences in the age groups infected by the different b lineages, with b/victoria infecting a younger population than b/yamagata, that could not be explained by potential prior exposure. | 2016 | 26848118 |
mesenteric thrombosis complicating influenza b infection. | 2016 | 26836382 | |
influenza b burden in latin america and potential benefits of the new quadrivalent vaccines. | 2016 | 26803330 | |
thyroid storm presenting as psychosis: masked by diabetic ketoacidosis. | while extremely uncommon, diabetic ketoacidosis (dka) and thyroid storm (ts) are endocrine emergencies that can coexist. we describe a case with a confounding clinical presentation that identifies these two emergencies within the setting of sepsis and influenza. | 2016 | 27609719 |
an interesting case of viral pericarditis. | a previously healthy 14-year-old girl presented to the emergency department with a 3-day history of upper respiratory symptoms and 2 syncopal episodes. she was initially febrile, tachycardic, and tachypneic; the initial electrocardiogram showed diffuse t-wave inversions and right atrial enlargement. there was no pericardial effusion on bedside and formal echocardiography; the latter, however, revealed a hyperechogenic pericardium. a viral swab was positive for influenza b. treatment with intrave ... | 2016 | 26181497 |
a comprehensive review of the epidemiology and disease burden of influenza b in 9 european countries. | this review was undertaken to consolidate information on the epidemiology and burden of influenza b, as well as the circulation patterns of influenza b lineage in 9 european countries. following a comprehensive search of peer-reviewed and gray literature sources, we found that published data on influenza b epidemiology and burden are scarce. surveillance data show frequent co-circulation of both influenza b lineages during influenza seasons, but little is known about its impact, especially in ad ... | 2016 | 26890005 |
infant vaccination timing: beyond traditional coverage metrics for maximizing impact of vaccine programs, an example from southern nepal. | immunization programs currently measure coverage by assessing the proportion of children 12-24 months who have been immunized but this does not address the important question of when the scheduled vaccines were administered. data capturing the timing of vaccination in first 6 months, when severe disease is most likely to occur, are limited. | 2016 | 26788880 |
influenza b-associated acute necrotizing encephalopathy of childhood: a report from north america. | 2016 | 26627900 | |
assessment of potential public health impact of a quadrivalent inactivated influenza vaccine in thailand. | each year, an influenza b strain representing only one influenza b lineage is included in the trivalent inactivated influenza vaccine (iiv3); a mismatch between the selected lineage and circulating viruses can result in suboptimal vaccine effectiveness. we modeled the added potential public health impact of a quadrivalent inactivated influenza vaccine (iiv4) that includes strains from both influenza b lineages compared to iiv3 on influenza-associated morbidity and mortality in thailand. | 2016 | 26588892 |
vaccinations and childhood type 1 diabetes mellitus: a meta-analysis of observational studies. | the aim of this study was to investigate the association between routine vaccinations and the risk of childhood type 1 diabetes mellitus by systematically reviewing the published literature and performing meta-analyses where possible. | 2016 | 26564178 |
[increased sensitivity of 1-layer tissue cultures to influenza b and se-polyoma viruses in a modified method of infection of cell suspensions]. | 2016 | 13884917 | |
[influenza b viruses]. | 2016 | 13915025 | |
[identification of atypical strains of a virus probably belonging to influenza b isolated during the 1962 epidemic]. | 2016 | 13978103 | |
retrospective public health impact of a quadrivalent influenza vaccine in the united states. | vaccination is an effective preventive strategy against influenza. however, current trivalent influenza vaccines (tivs) contain only one of the two influenza b lineages that circulate each year. vaccine mismatches are frequent because predicting which one will predominate is difficult. recently licensed quadrivalent influenza vaccines (qivs) containing the two b lineages should address this issue. our study estimates their impact by assessing what would have been the us public health benefit of ... | 2015 | 26256294 |
an assessment of the expected cost-effectiveness of quadrivalent influenza vaccines in ontario, canada using a static model. | ontario, canada, immunizes against influenza using a trivalent inactivated influenza vaccine (iiv3) under a universal influenza immunization program (uiip). the uiip offers iiv3 free-of-charge to all ontarians over 6 months of age. a newly approved quadrivalent inactivated influenza vaccine (iiv4) offers wider protection against influenza b disease. we explored the expected cost-utility and budget impact of replacing iiv3 with iiv4, within the context of ontario's uiip, using a probabilistic and ... | 2015 | 26222538 |
a case of polymyalgia rheumatica following influenza b infection. | polymyalgia rheumatica (pmr) is relatively common among the elderly, and is characterized by multiple body aches with an elevated erythrocyte sedimentation rate. even though the etiology of pmr remains unknown, a number of infectious agents have been suggested to cause pmr. also, there are reports of pmr after influenza vaccination. the exact role of influenza vaccination on the development of pmr remains unknown, but may be associated with specific human leukocyte antigens (hlas), such as hla-d ... | 2015 | 26527896 |
cost-effectiveness evaluation of quadrivalent influenza vaccines for seasonal influenza prevention: a dynamic modeling study of canada and the united kingdom. | the adoption of quadrivalent influenza vaccine (qiv) to replace trivalent influenza vaccine (tiv) in immunization programs is growing worldwide, thus helping to address the problem of influenza b lineage mismatch. however, the price per dose of qiv is higher than that of tiv. in such circumstances, cost-effectiveness analyses provide important and relevant information to inform national health recommendations and implementation decisions. this analysis assessed potential vaccine impacts and cost ... | 2015 | 26503131 |
a novel dynamic model for health economic analysis of influenza vaccination in the elderly. | new vaccines are being developed to improve the efficacy of seasonal influenza immunization in elderly persons aged ≥65 years. these products require clinical and economic evaluation to aid policy decisions. | 2015 | 26350238 |
non-inferiority of mammalian cell-derived quadrivalent subunit influenza virus vaccines compared to trivalent subunit influenza virus vaccines in healthy children: a phase iii randomized, multicenter, double-blind clinical trial. | the safety and immunogenicity of mammalian cell-derived quadrivalent influenza vaccine (qivc) as compared with trivalent influenza vaccines (tiv1c/tiv2c) was evaluated in children aged ≥4 to <18 years. | 2015 | 26585940 |
mapping epitopes of u1-70k autoantibodies at single-amino acid resolution. | the mechanisms underlying development of ribonucleoprotein (rnp) autoantibodies are unclear. the u1-70k protein is the predominant target of rnp autoantibodies, and the rna binding domain has been shown to be the immunodominant autoantigenic region of u1-70k, although the specific epitopes are not known. to precisely map u1-70k epitopes, we developed silicon-based peptide microarrays with >5700 features, corresponding to 843 unique peptides derived from the u1-70k protein. the microarrays featur ... | 2015 | 26333287 |
immunogenicity and safety of a cell culture-derived inactivated trivalent influenza vaccine (nbp607): a randomized, double-blind, multi-center, phase 3 clinical trial. | cell culture-derived influenza vaccines (ccivs) have several important advantages over egg-based influenza vaccines, including shorter production time, better preservation of wild-type virus antigenicity and large-scale production capacity. | 2015 | 26314625 |
increased prevalence of influenza b/victoria lineage viruses during early stages of the 2015 influenza season in new south wales, australia: implications for vaccination and planning. | during the early weeks of the 2015 australian influenza season, influenza b accounted for 67% (821/1,234) of all positive influenza tests in new south wales. of 81 successive influenza b viruses characterised, 33 (41%) were from children aged < 16 years; 23/81 (28%) belonged to the b/victoria lineage. this lineage is not contained in the southern hemisphere's 2015 trivalent influenza vaccine. the significant b/victoria lineage activity in the southern hemisphere suggests that the quadrivalent va ... | 2015 | 26290426 |
[susceptibility of influenza b viruses to neuraminidase inhibitors isolated during 2013-2014 influenza season in mainland china]. | data based on the antiviral-resistant phenotyping characteristics of 884 influenza b viruses circulating in mainland china from october 2013 to march 2014 were analyzed to assess the susceptibility of influenza b viruses to neuraminidase inhibitors. all 884 viruses were sensitive to oseltamivir; two viruses (0.23%) had reduced sensitivity to zanamivir and all other viruses were sensitive to zanamivir. among the 38 viruses with a b/victoria lineage, b/shandong-kuiwen/1195/2014 exhibited a half-ma ... | 2015 | 26164940 |
reassortment between influenza b lineages and the emergence of a coadapted pb1-pb2-ha gene complex. | influenza b viruses make a considerable contribution to morbidity attributed to seasonal influenza. currently circulating influenza b isolates are known to belong to two antigenically distinct lineages referred to as b/victoria and b/yamagata. frequent exchange of genomic segments of these two lineages has been noted in the past, but the observed patterns of reassortment have not been formalized in detail. we investigate interlineage reassortments by comparing phylogenetic trees across genomic s ... | 2015 | 25323575 |
interactions of lipids and detergents with a viral ion channel protein: molecular dynamics simulation studies. | structural studies of membrane proteins have highlighted the likely influence of membrane mimetic environments (i.e., lipid bilayers versus detergent micelles) on the conformation and dynamics of small α-helical membrane proteins. we have used molecular dynamics simulations to compare the conformational dynamics of bm2 (a small α-helical protein from the membrane of influenza b) in a model phospholipid bilayer environment with its behavior in protein-detergent complexes with either the zwitterio ... | 2015 | 25286030 |
only a touch of the flu? the simultaneous manifestation of acute necrotizing encephalopathy in two consanguineous patients. | this case report describes the simultaneous manifestation of acute necrotizing encephalopathy in 2 consanguineous patients after infection with influenza b based on the autosomal dominant missense mutation of the ranbp2-gene. differential diagnosis of acute encephalopathy, clinical and radiological clues, and treatment strategies are outlined. | 2015 | 26110162 |
toward improved influenza control through vaccination. | in august 27/2014, slipe organized the master class "towards improved influenza control through vaccination", a panel with international influenza experts who shared their understanding of the disease and the control measures available, focusing on the most recent information about this serious diseases. in this report dr falleiros and dr bricks summarized the following topics: global influenza epidemiology, presented by dr puig-barbera; influenza vaccine recommendations and coverage in latin am ... | 2015 | 26065453 |
surveillance of acute respiratory infections among outpatients: a pilot study in isfahan city. | considering that there was not any regional survey in isfahan, iran regarding the epidemiology of acute respiratory tract infections (arti) in different age groups of general population, the aim of this study was to determine the epidemiologic feature of artis in isfahan using multiplex polymerase chain reaction (pcr) method. | 2015 | 25983761 |
plastic bronchitis arising from solitary influenza b infection: a report of two cases in children. | plastic bronchitis (pb) is characterized by thick, inspissated, tracheobronchial casts. it is classified as either inflammatory or acellular based on the content of the endobronchial casts. pb has never been reported in a healthy child with solitary influenza b infection. this study is a retrospective case series of two children who presented to our institution in acute respiratory distress. emergency rigid bronchoscopy was performed with extraction of casts from the l mainstem bronchus in both ... | 2015 | 25957780 |
a case of acute kidney injury and disseminated intravascular coagulation associated with influenza b viral infection. | there are few reports of acute kidney injury (aki) associated with influenza viral infection. we treated a case of aki that developed after an influenza b viral infection. a 35-year-old man visited a local physician for a fever and was diagnosed with influenza b. he was prescribed laninamivir, then returned to the physician 5 days later with dyspnea and was referred to hospital a. upon admission, respiratory arrest developed, for which he received tracheal intubation and mechanical ventilation. ... | 2015 | 28509280 |
oseltamivir-warfarin interaction in hypoplastic left heart syndrome: case report and review. | an 8-year-old boy with hypoplastic left heart syndrome with a previous history of thrombosis within the inferior vena cava receiving stable warfarin dosing for anticoagulation was diagnosed with influenza b. he was subsequently placed on oseltamivir therapy according to the centers for disease control and prevention clinical practice guidelines. during the hospitalization, his international normalized ratio steadily increased to supratherapeutic levels and returned to baseline after discontinuat ... | 2015 | 25917992 |
[benign acute childhood myositis as a complication of influenza b and its differential diagnosis]. | analysis of the course of benign acute childhood myosistis during the influenza epidemic of 2012/2013, with a focus on the clinical specificities, laboratory findings, diagnostic and therapeutic approaches, and prognosis. | 2015 | 26795228 |
live attenuated influenza vaccine tetravalent: a clinical review. | live attenuated influenza vaccine (laiv) has been available as a trivalent formulation in the eu since 2012. influenza b strains from two lineages have co-circulated outside asia in europe, israel and north america since the early 2000s. the trivalent vaccine contained a single influenza b lineage virus chosen primarily on the basis of the previous year's circulating lineage. failure to align the vaccine virus with the circulating virus leaves even vaccinated patients, particularly children, at ... | 2015 | 25864428 |
cost-effectiveness of quadrivalent influenza vaccine in hong kong - a decision analysis. | trivalent influenza vaccine (tiv) selects one of the 2 co-circulating influenza b lineages whereas quadrivalent influenza vaccine (qiv) includes both lineages. we examined potential cost-effectiveness of qiv versus tiv from perspectives of healthcare provider and society of hong kong. a decision tree was designed to simulate the outcomes of qiv vs. tiv in 6 age groups: 0-4 years, 5-9 years, 10-14 years, 15-64 years, 65-79 y and ≥80 years. direct cost alone, direct and indirect costs, and quality ... | 2015 | 25714506 |