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involvement of spleen components of mature fowl in the primary and secondary humoral antibody response following experimental eds'76 virus infection.cellular changes in spleens of mature fowl in relation to both the primary and secondary humoral antibody response following experimental eds'76 virus infection were studied. the influence of splenectomy on humoral antibody response was also examined. experimental fowl had been naturally infected with fowl adenovirus (fav) but did not possess precipitins to these viruses at the time of eds'76 virus infection. since eds'76 infection provokes a recall of the group antibody to fav, this infection s ...19863014709
pathology of avian adenovirus serotypes in the presence of escherichia coli in infectious-bursal-disease-virus-infected specific-pathogen-free chickens.ten strains of adenovirus representing 10 serotypes were administered intratracheally to 3-week-old specific-pathogen-free chickens, which also received 2.5 x 10(5) colony-forming units of a pathogenic escherichia coli intranasally. those birds had been inoculated by eye drop at 1 day of age with a virulent infectious bursal disease virus (ibdv). controls consisted of groups of chickens inoculated with: (a) ibdv and e. coli, (b) ibdv only, (c) e. coli only, and (d) no virus or e. coli. gross pul ...19863015109
a possible viral candidate for the aetiology of turkey rhinotracheitis. 19863006318
[detection of avian adenovirus antigens in chicken cell cultures]. 19863009130
va rnas from avian and human adenoviruses: dramatic differences in length, sequence, and gene location.human adenoviruses encode low-molecular-weight rnas, so-called va rnas, which are transcribed by rna polymerase iii. these rnas are required for an efficient translation of viral mrnas late after infection. the genes for the va rnas in the genome of celo virus were mapped and characterized. the results showed a number of surprising differences between celo virus and human adenovirus type 2 (ad2). thus, the celo virus genome encoded only one va rna species, in contrast to human ad2, which encoded ...19863009871
viruses associated with turkey rhinotracheitis in great britain. 19863012855
[stability of the structure and antigenic determinants of adenovirus type 1 native hexon to proteases].hexon capsomers of human adenovirus type 1 (h1) labeled by iodine 125 were digested in a native state (trimers) by trypsin, chymotrypsin or papain, and the resulting hydrolysates were analyzed by sds-page. in each case, a discrete and temporally stable pattern of relatively large fragments was revealed. the degree of hexon polypeptide hydrolysis was maximal for papain, intermediate for chymotrypsin and minimal for trypsin, the largest fragments in the digest being 32, 40 and 80 kd, respectively. ...19862429709
experimental infection of laying hens with an adenovirus isolated from ducks showing eds symptoms. 19853012988
adenovirus-like particles associated with intranuclear inclusion bodies in the kidney of a common murre (uria aalge).adenovirus-like particles were identified by transmission electron microscopy in intranuclear inclusion bodies in the renal collecting tubules of a male common murre. the bird had been trapped in an oil-spill and had been cleaned and held in captivity before euthanasia. the presence of the virus appeared to be causing little or no renal disease. it is thought that this bird suffered activation of a latent viral infection as a result of the stress of oil intoxication, handling, and confinement.19852985038
purification method for the avian adeno-associated virus.avian adeno-associated virus (aaav) pre-purified by uvasol extraction, one discontinuous and two cscl equilibrium density gradients, was still considerably contaminated with avian adenovirus (celo strain). four different approaches were investigated in attempts to improve the elimination of the contaminating celo virus. the contaminations were assayed by double immunodiffusion and indirect immunofluorescence. the immunoprecipitation of celo virus with antiserum and protein a-sepharose is the mos ...19852989313
increase in dna polymerase alpha in nuclei of chicken kidney cells after avian adenovirus infection.dna polymerase activity which sedimented at about 7s in the presence of 0.5m kc1 increased in cultured kidney cells after infection with a chicken embryo lethal orphan virus, an avian adenovirus. elevated levels of antigenic material to a monoclonal antibody specific for the large subunit of chicken dna polymerase alpha were also detected in nuclei of virus-infected cells by an immunofluorescence method. since the elevations of dna polymerase activity and of nuclear immunofluorescence was not in ...19852991062
an investigation of vertical transmission of a fowl adenovirus serotype 8. 19852992432
use of egg yolk to determine antibody levels in chickens inoculated with a hemagglutinating duck adenovirus (adenovirus 127-like).chickens were experimentally infected with a duck adenovirus that has been shown to be serologically indistinguishable from adenovirus 127. sera and eggs were collected at intervals after exposure for antibody determination by the hemagglutination-inhibition (hi) test, the enzyme-linked immunosorbent assay (elisa), and the immunodiffusion (id) test. egg yolks were processed for use in the serological tests by (a) dilution in phosphate-buffered saline (pbs), (b) extraction of the water-soluble fr ...19852992438
haemagglutinating adenovirus in victorian chicken flocks 1980-83. 19852994619
grouping of fowl adenoviruses based upon the restriction patterns of dna generated by bamhi and hindiii.based upon the restriction patterns of dnas generated by restriction endonucleases bamhi and hindiii, 17 fowl adenovirus strains representing 11 serotypes were placed into 5 groups: group a, strains celo and 112; group b, strains 340 and tr-22; group c, strains kr-5 and c-2b; group d, strains 380, 685, sr-48, sr-49, and a-2; and group e, strains yr-36, cr-119, x-11, h-6, tr-59, and 764.19846090337
viral hepatitis in domestic geese in saskatchewan.up to 25% mortality occurred in goslings between 8 and 28 days of age in three sequential hatches from a closed breeder flock on a single farm. the birds died suddenly with minor ascites, subcutaneous and myocardial hemorrhages, and pale, mottled livers with petechial hemorrhages. liver necrosis associated with intranuclear inclusion bodies was seen on histology. virus particles were found on examination of the inclusion bodies with an electron microscope. liver homogenates caused variable morta ...19846091609
the structural proteins of chick embryo lethal orphan virus (fowl adenovirus type 1).chick embryo lethal orphan (celo) virus (fowl adenovirus type 1) contains at least 14 structural proteins with polypeptide molecular weights ranging from 100k to about 6k. a nomenclature of the celo virion polypeptides is presented and the molar proportion of each polypeptide has been estimated. the celo virus pentons were specifically released from the virion by dialysis against borate-based calcium-magnesium saline. the penton base (polypeptide iii, mol. wt. 92k) and two fibres were separated, ...19846092524
dna-binding proteins of chick embryo lethal orphan virus: lack of complementation between early proteins of avian and human adenoviruses.chick cells infected by chick embryo lethal orphan (celo) virus (fowl adenovirus type 1) contained four prominent virus-specific, structurally related dna-binding proteins with mol. wt. of 74k, 64k, 56k, 52k, and two minor forms. the celo virus dna-binding proteins were phosphorylated, delayed-early nuclear proteins. celo virus early proteins were expressed in bhk cells, but did not complement human adenovirus type 5 mutants with lesions e1a, e2a or e2b. moreover, celo virus dna-binding proteins ...19846092525
structural organization and polypeptide composition of the avian adenovirus core.celo virus (fowl adenovirus 1) contained three core polypeptides of molecular weights 20,000, 12,000, and 9,500. the core was similar to that of human adenoviruses, with some evidence of compact subcore domains. micrococcal nuclease digestion of celo virus cores produced a smear of dna fragments of gradually decreasing size, with no nucleosome subunit or repeat pattern. moreover, when digested cores were analyzed without protease treatment, there was again no evidence of a nucleosome substructur ...19846092686
adenovirus-like agent in the bursa of fabricius of herring gulls (larus argentatus pontoppidan) from newfoundland, canada. 19846092730
purification and hemagglutinating properties of egg drop syndrome 1976 virus.we purified three populations of virus particles, f7, f9 and f17, with buoyant densities of 1.34, 1.33 and 1.29 g/ml, respectively, in cscl equilibrium density gradients from cultures of chick embryo liver cells infected with the h-162 strain of the virus of egg drop syndrome 1976. f9 particles were infectious complete virions and most f17 particles were empty particles. f7 particles were less infectious, and had little capacity of hemagglutination (ha). ha titers were the same at 4 degrees and ...19846322727
[amplification of virocellular sequences avian adenovirus-induced tumor cells].adenovirus-induced tumor cells (including transformed cells) examined contain a part of the viral genome. however, number of molecules of the virus dna persisting in a cell varies in the tumor cell lines. tumor cell lines which were induced by celo virus, an avian adenovirus, carried the virus dna in the range from 1 to 920 molecules per cell. of the tumor cell line which carried large numbers of molecules of the virus dnr, one tumor cell line, rt 13 (160 molecules/cell), was studied both at the ...19846324694
evidence of exposure of waterfowl and other aquatic birds to the hemagglutinating duck adenovirus identical to eds-76 virus.serum and fecal samples from 12 species of aquatic birds were studied for evidence of exposure to a hemagglutinating duck adenovirus (dav). dav is serologically indistinguishable from egg-drop syndrome-76 virus. a total of 285 serum samples were tested by the hemagglutination-inhibition (hi) test. forty-two percent of the birds had hi antibodies, with titers ranging from 8 to 256. wild ducks showed the highest frequency of antibodies (56%) while in coots and grebes, antibody was less frequent, 3 ...19846325725
pathogenicity of various adenovirus serotypes in the presence of escherichia coli in chickens.ten strains of adenovirus representing 10 serotypes were administered intratracheally to 3-week-old specific-pathogen-free chickens, which also received 2.9 x 10(5) colony-forming units of a pathogenic escherichia coli intranasally. one group was given only e. coli, and one was retained as an uninoculated control. gross pathologic alterations post-mortem were minimal and limited to multiple scattered, pale areas in the lungs of an occasional chicken in various groups. histopathologic changes in ...19846326735
experimental infection of laying chickens with adenovirus 127 and with a related virus isolated from ducks.infection of commercially reared white leghorn and white rock hens with adenovirus 127 was associated with decreases in total egg production, external egg quality, egg weight (p less than 0.01), and eggshell thickness (p less than 0.01). the egg-production and egg-quality disturbances were transient, and production returned to normal approximately 4 weeks postinfection. infection of white leghorns with a hemagglutinating adenovirus isolated from missouri ducks did not adversely affect egg produc ...19846326738
efficacy and safety of a cell-culture live virus vaccine for hemorrhagic enteritis of turkeys: laboratory studies.poults free from hemorrhagic enteritis (he) antibody were vaccinated by gavage at 1 day or 2 weeks of age with a live he vaccine virus that had been propagated in a marek's disease (md)-induced b-lymphoblastoid cell line of turkey origin. vaccinated and unvaccinated poults were challenged with a virulent he virus at various times postvaccination. one hundred tissue-culture-infectious doses of the vaccine virus per poult were sufficient to induce a serological response as well as to protect poult ...19846326740
the use of an enzyme-linked immunosorbent assay to detect igg antibodies to serotype-specific and group-specific antigens of fowl adenovirus serotypes 2, 3 and 4.a sensitive enzyme-linked immunosorbent assay (elisa) has been developed which can detect serotype and group-specific antibodies (igg) to fowl adenovirus serotypes 2, 3 and 4. the chickens produced principally type-specific antibodies after a single oral inoculation of virus which enabled that strain to be identified by the elisa. however, inoculation of an heterologous serotype, although inducing strain-specific antibodies to itself, also induced high levels of antibody to the group-specific an ...19836330150
isolation of chicken anemia agent with mdcc-msb1 cells from chickens in the field.an attempt was made to isolate chicken anemia agent (caa) from chickens suffering from anemia in the field by using mdcc - msb1 , which was an established cell line derived from marek's disease lymphoma. when 99 chickens of 15 flocks were examined, caa was isolated from 58 chickens of 12 flocks. the rate of caa isolation with mdcc - msb1 cells was almost the same as that determined by an in vivo method by chick inoculation. it was shown that caa was more closely concerned with anemic diseases of ...19836330575
isolation of fowl adenovirus from tendons and tendon sheaths of chickens with leg weakness. 19836314026
comparison of enzyme-linked immunosorbent assay with hemagglutination-inhibition and immunodiffusion tests for detection of antibodies to a hemagglutinating duck adenovirus in chickens.an indirect enzyme-linked immunosorbent assay (elisa) for the detection of antibody to a hemagglutinating duck adenovirus is described. optimum conditions for the test were determined, and the system was compared with procedures currently used. experimentally infected chickens were assayed for specific antibody by elisa, hemagglutination inhibition (hi), and immunodiffusion (id). the elisa was a sensitive and reliable method for detecting antibody, although positive titers were not always in agr ...19836314978
hemorrhagic enteritis in captive american kestrels (falco sparverius).hemorrhagic enteritis and hepatitis of suspected adenovirus etiology were the apparent cause of death of nine captive american kestrels. cloacal hemorrhage was the only prominent gross lesion: disseminated hepatocellular necrosis, and intranuclear inclusion bodies were evident microscopically. electron microscopy revealed numerous adenovirus-like particles associated with the hepatic lesions. attempts to serologically identify the agent were unsuccessful.19836315970
isolation and identification of avian adenovirus from a case of inclusion body hepatitis in broilers in malaysia. 19836317137
isolation of the virus of egg drop syndrome 1976 (eds-76) in a japanese outbreak. 19836317930
comparative sequence analysis of the inverted terminal repetition in the genomes of animal and avian adenoviruses.the nucleotide sequences at the inverted terminal repetitions from two animal adenoviruses, infectious canine hepatitis virus and equine adenovirus, and from one avian adenovirus, celo, were analyzed. dnas from infectious canine hepatitis virus and equine adenovirus contain a homologous region which is 23 nucleotides long from the terminus. the first 17 nucleotides of this region are identical to the ones in human adenovirus type 2 dna. the striking homologous sequence of 14 nucleotides, conserv ...19836301151
effects of experimental infection of fowl with eds'76 virus, infectious bronchitis virus and/or fowl adenovirus on laying performance. 19836302974
a comparison of the terminal protein and hexon polypeptides of avian and human adenoviruses.it was found that the virion terminal protein of chick embryo lethal orphan (celo) virus had a molecular weight of 46,000, and the hexon a molecular weight of 100,000. 125i-labelled tryptic and chymotryptic peptide maps of the hexons and terminal proteins from celo virus and human adenovirus type 5 (ad5) differed. however, limited proteolysis of celo virus and ad5 terminal proteins by protease v8 showed similarities which were not detected in the case of the two hexons.19836304239
biochemical studies of celo virus: an oncogenic avian adenovirus. 19836297961
[oncogenicity of adenoviruses]. 19826298900
survey on antibody against hemorrhagic enteritis virus among chicken flocks in japan.a survey on antibody against hemorrhagic enteritis virus was conducted by the agar-gel precipitation test among chicken flocks in japan. the positive rate of individual serum samples increased with the advance in age of chickens. ninety-one of 181 sera (50%) and 23 of 33 flocks (79%) were positive among chickens more than 181 days old.19826312333
[a contribution to the egg drop syndrome of laying chickens. 1: clinical and serological findings (author's transl)]. 19826280951
[a contribution to the egg drop syndrome of laying chickens. 2. macroscopic and histological findings (author's transl)]. 19826280952
isolation of a haemagglutinating adenovirus from commercial ducks. 19826281959
sequence homology between avian and human adenoviruses.studies of hybridization between fowl adenovirus type 1 (chicken embryo lethal orphan virus) dna and human adenovirus type 2 dna revealed two short but distinct regions which cross-hybridized under stringent conditions. one of the homologous regions was located between map positions 18.1 and 19.3 and did not correspond to any gene recognized so far. the second region mapped in the hexon gene between position 57 and 58.19826283148
is avian adeno-associated virus an endogenous virus of chicken cells?the adeno-associated viruses (aav) are defective parvoviruses which produce infective progeny only in cells co-infected with a 'helper' adenovirus (ad). both human and simian aav have been recovered from human and simian primary cell cultures following their inoculation with 'aav-free' ad. whereas some studies have suggested that aav exists in a latent state in these cells, others have indicated that the aav genome is capable of establishing and maintaining a latent state in defined laboratory c ...19826285201
the effect of incubation temperature on the propagation of duck adenovirus (virus 127-like) in duck and chicken cells.duck adenovirus (cornell strain) was propagated in duck and chicken embryo cells at 37.5 c and at 40 c. in duck cells, virus levels, as indicated by ha titers, peaked earlier at 40 c than at 37.5 c. high titers were eventually observed in duck cells at both temperatures. in chicken embryo fibroblasts, no titers were observed at 37.5 c, whereas low titers were observed at 40 c. evidence of virus propagation was not detected in chicken embryo liver and kidney cells.19826285881
the fibers of fowl adenoviruses.purified virions from 14 strains of fowl adenoviruses (fav) representing 11 serotypes were examined by electron microscopy. pentons of all strains turned out to have two projections at their penton bases. it is shown that the double fibers also protrude from the intact virion. the length of both fibers was measured on free pentons and is given for each serotype. double fibers seem to be a common feature of fav. the serologically unrelated egg drop syndrome (eds) avian adenovirus strain 127 was c ...19826287974
a common sequence in the inverted terminal repetitions of human and avian adenoviruses.the termini of the avian chick embryo lethal orphan (celo) virus dna have been sequenced. the results revealed a 63-bp-long inverted terminal repetition (itr) which shared the sequence ataata with all adenovirus termini, thus far analyzed. the celo virus itr differed from those of the mammalian adenoviruses in two major aspects: (i) it is not a perfect duplication; (ii) it begins with a 5'-guanylic acid residue instead of the cytidylic acid normally observed in adenoviruses.19826290323
survey on antibody against egg drop syndrome-1976 virus among chicken flocks in japan.a survey on antibody against egg drop syndrome-1976 virus was carried out with 4,518 sera collected from 667 flocks over a period from 1971 to 1981 in japan. antibody-positive sera were initially detected in 1976, and the number of positive sera increased suddenly in 1979, and a total of 270 sera from 23 flocks were positive. the positive flocks consisted of 20 broiler parent flocks belonging to 9 farms and 3 layer flocks belonging to 2 farms. it seemed from the results of this survey that the p ...19826290899
a survey of serum antibody to avian haemagglutinating adenovirus in queensland poultry flocks. 19826291500
serological examination and egg production of progeny of fowl experimentally infected with egg drop syndrome 1976 virus.following eds'76 virus (bc14 virus) infection of breeder chickens by the conjunctival route, vertical transmission occurred in the first week after infection. in the progeny which had been infected with eds'76 virus by the vertical route, increasing haemagglutination inhibiting (hi) titres to bc14 virus and increasing numbers of birds with hi titres were observed from 3 weeks to 15 weeks of age. sixty-one per cent of the hens and 77 per cent of the cocks had 2 log hi bc14 virus titres exceeding ...19826293156
lesions induced in the respiratory tract of chickens by serologically different adenoviruses.ten strains of adenovirus representing 10 serotypes were administered intratracheally to two groups of 3-week-old specific-pathogen-free chickens. birds in group 1 were given only adenovirus. birds in group 2 were inoculated with a virulent infectious bursal disease virus (ibdv) by eye-drop at one day of age as well as with the adenovirus at 3 weeks. the chicks were examined daily. respiratory rales were observed in some birds with the dual infection. gross pathologic alterations were minimal an ...19826293441
heat inactivation of a duck adenovirus serologically indistinguishable from adenovirus 127. 19826293448
pulmonary congestion and edema (marble spleen disease) of chickens produced by group ii avian adenovirus."marble spleen disease" of chickens was diagnosed in 22-week-old chickens. total mortality was 8.9%. deaths occurred over a period of 2 months. gross lesions included pulmonary congestion, splenomegaly, hepatomegaly, and congestion of egg follicles. microscopic lesions included pulmonary congestion and edema, and reticuloendothelial cell hyperplasia of the spleen with concurrent white-pulp necrosis and lymphocyte depletion. the pulmonary lesions were of sufficient intensity to have caused the de ...19826293450
serological cross-reactivity of avian adenovirus serotypes in an enzyme-linked immunosorbent assay.fowl adenoviruses free of avian adenovirus-associated virus, representing 10 serotypes, were tested for cross-reactivity in an enzyme-linked immunosorbent assay (elisa). all antigens and antisera prepared in chickens, along with uninfected control antigen and normal chicken serum, were reacted in a checkerboard pattern with elisa. there was considerable cross-reactivity among all serotypes tested. homologous reactions were generally, but not always, stronger than heterologous reactions. elisa wa ...19826297452
the oncogenicity of avian adenoviruses. iii. in situ dna hybridization of tumor line cells localized a large number of a virocellular sequence in few chromosomes. 19826278719
adenovirus-related rna sequences in human neurogenic tumours.thirty two human tumours, mainly neurogenic, have been investigated for the presence of adenovirus-related rna sequences. 3h-labelled tumour virus dna probes derived from human adenoviruses types 2 and 12, bovine adenovirus type 3, and avian adenovirus celo were hybridized in-situ on tumour kryostat sections under conditions that detect complementary rna. tumour virus-related rna was detected in 62% of all tumours tested, but was not detectable in normal human brain tissues. expression of tumour ...19826278813
characterization of two strains of fowl adenovirus type 1.an adenovirus isolated from the trachea of a chicken with respiratory disease was compared with an isolate obtained from the trachea of a clinically normal chicken. the isolates caused similar respiratory disease experimentally but differed in plaque morphology and fluorescent-antibody reactions in infected chick embryos.19816279068
histopathology of avian adenovirus group ii splenomegaly of chickens.chickens were infected with an avian adenovirus group ii isolate previously obtained from chickens exhibiting splenomegaly in commercial broiler flocks. the isolate was inoculated orally in 6-week-old experimental chickens, which were euthanatized and necropsied 6 days postinoculation. the primary gross lesions found were splenomegaly and splenic mottling. numerous tissues from 12 chickens were taken for histologic evaluation. histologic lesions included splenic reticuloendothelial cell hyperpla ...19816279073
[serological studies for the presence of hemagglutination-inhibiting antibodies against the "egg-drop syndrome 76" virus in south wuerttemberg]. 19816268058
studies on the egg-drop syndrome. 19816269409
short communications. 19816270874
isolation of haemagglutinating adeno-like virus related to virus 127 from an australian poultry flock with an egg drop syndrome.an egg drop syndrome within australian broiler poultry is described. the syndrome was characterised by delayed onset of laying, a lower peak in egg production and a drop in egg production shortly after reaching peak production. antibody to virus 127 was detected in 102 of 106 fowl serums tested. two haemagglutinating viruses were isolated from one affected flock and one was subjected to further study. it was adenovirus-like on electron-microscopic examination and haemagglutination was not inhibi ...19816271113
studies on egg drop syndrome (eds) and chick embryo lethal orphan (celo) avian adenovirus dnas by restriction endonucleases.restriction endonucleases ecori, bamhi, hindiii, bgli, bglii, hpai and psti recognized 48 cleavage sites in eds adenovirus strain b8/78 dna, whereas celo virus dna was cut into 61 fragments by the same enzymes. no similarity could be detected in the restriction patterns obtained from the two avian adenoviruses. the calculated mol. wt. of b8/78 dna was 22.6 x 10(6) [about 34.2 kilobases (kb)] and that of celo virus dna was 28.9 x 10(6) (about 43.7 kb). the fragments generated from b8/78 dna by ec ...19816271918
isolation of viruses from outbreaks of suspected tenosynovitis (viral arthritis) in chickens.specimens from the legs of chickens from 84 outbreaks of suspected tenosynovitis were examined for the presence of viruses by culture in chick embryo lung or liver cell monolayers. all samples were from broilers or broiler breeders, ranging in age from dead-in-shell embryos to 36 weeks old. twenty-five outbreaks (29.8 per cent) yielded viruses of which 12 were reoviruses alone, 12 adenoviruses alone, and one, a mixture of both types of virus. rupture of the gastrocnemius tendon was seen in 12 ou ...19816273982
outbreaks of egg-drop syndrome-1976 in japan and its etiological agent.a condition similar to egg-drop syndrome-1976 (eds-76) occurred in 14 broiler breeding flocks in 2 farms in japan from december 1978 to january 1980, and it was diagnosed as eds-76 by serologic and virological investigations. egg production fell suddenly when the hens were 30 to 55 weeks of age, and the depression lasted 3 to 7 weeks. production fell 6 to 25%. depressed egg production was accompanied by the laying of shell-less, soft-shelled, and thin-shelled eggs associated with loss of egg-she ...19816274291
pathogenicity and distribution of egg-drop syndrome-1976 virus (jpa-1) in inoculated laying hens.rhode island red laying hens that lacked hemagglutination-inhibition antibody were inoculated orally with the jpa-1 strain of adenovirus isolated from a flock affected with egg-drop syndrome-1976 in japan and observed for 80 days. inoculated hens laid abnormal eggs, such as shell-less, soft-shelled, and cracked eggs and those with loss of pigmentation, from 8 days postinoculation (pi). fifteen out of 16 hens laid abnormal eggs. egg-production rte fell from 94% to 50% between 13 and 16 days pi. w ...19816274292
adenovirus isolation and serology from wild bobwhite quail (colinus virginianus). 19816274295
egg transmission of avian adenovirus-associated virus and celo virus during experimental infections. 19816275751
avian adenoviruses and avian reoviruses isolated from diseased chickens. 19816275831
growth of adeno-associated virus in embryonated eggs. 19816262350
[study of viral contamination of japanese quail and cell cultures from their embryos].agents identified as adenovirus celo were isolated from organ suspensions of 1500 japanese quails (jq) in 4 experiments. no contaminating viruses were found in examinations of cell cultures prepared from the kidneys of 80 jq. negative results were obtained in examinations of 163 antigens from jq organs in the cofal test. agents identified as mycoplasma were isolated in 16 cases from 736 specimens of the virus-containing fluid used for manufacture of measles virus. according to the results of the ...19816266156
hemorrhagic enteritis: virus distribution and sequential development of antibody in turkeys.turkeys poults were inoculated intraperitoneally with hemorrhagic enteritis virus (hev) at 4-1/2 weeks of age. antibody response and sequential development of viral antigen in various tissues were monitored. an enzyme-linked immunosorbent assay (elisa) was developed to study antibody production, and immunoperoxidase staining was used to determined sites of localization of the viral antigens in tissues. results of elisa and immunodiffusion tests were compared. elisa detected antibody from day 3 p ...19816266390
turkey respiratory tract adenoviruses: oncogenic potential in neonatal hamsters (mesocricetus auratus).the tumorigenic properties of 3 turkey adenoviruses (cua, nc-k, and mst) isolated from turkeys with respiratory tract disease and injected into neonatal hamsters (mesocricetus auratus) have been determined. one of 30 adenovirus isolates (cua) induced tumors at the site of inoculation (subcutaneously or intracranially) in neonatal hamsters. the tumors were identified as fibrosarcomas and undifferentiated sarcomas. the tumors were found to be free of infective virus, but hamsters which had tumors ...19816267964
turkey respiratory tract adenoviruses: purification and some biochemical properties. 19816267965
turkey respiratory tract adenoviruses: in vitro infectivity of purified dna. 19816267966
some biological and physico-chemical properties of egg drop syndrome (eds) avian adenovirus strain b8/78.the eds adenovirus produced either in the allantoic cavity of embryonated duck eggs (a0 virus) or in chicken embryo liver cell cultures (tc0 virus) were comparatively studied by gradient ultracentrifugation in cscl. the a0 viral particles banded at densities of 1.36 and 1.31 g/ml, whereas, the tc0 viral particle were found at densities of 1.33 and 1.31 g/ml, respectively. the heavy populations contained infectious and hemagglutinating particles if they were not pelleted and sonicated before grad ...19816268020
avian haemagglutinating adenovirus antibodies in australian poultry. 19816282245
immunological relationship among adenoviruses of humans, simians, and nonprimates as determined by the neutralization test.98 antisera prepared against 25 simian adenoviruses and 31 adenovirus types from various nonprimate mammals and birds were tested for their ability to neutralize 35 human adenovirus prototypes. antisera to these human adenovirus types were similarly tested against 25 simian adenovirus prototypes. although very few reactions to the human adenoviruses were found in antisera to nonprimate viruses, cross-reactions among the simian and human adenoviruses were more frequent. the majority of these reac ...19816168602
egg production in relation to the results of a long term serological survey of 73 flocks of fowl.seventy-three flocks of fowl were tested at regular intervals for the presence of precipitins to fowl adenovirus (av) and infectious bronchitis virus (ibv), haemaggluinating inhibiting antibodies to bc in 14 virus, and of agglutinins to mycoplasma gallisepticum (m.g.) and mycoplasma synoviae (m.s.). in all the eight flocks affected with egg drop syndrome (eds '76), egg production problems were associated with increasing numbers of bc14 virus reactors and av reactors. in flocks showing production ...19806243424
[dynamics of maternal and post-infection adenovirus antibodies in fowl].the epizootological study of the adenovirus infections of poultry, conducted with the use of the serum virus-neutralizing test, gives a picture of the distribution of various serotypes of avian adenoviruses in the poultry population, or in the largest poultry stocks in czechoslovakia. there is a description of the occurrence of type-specific antibodies in serums of different age categories of poultry acting against seven available virus serotypes. an ascending trend in positive findings of adeno ...19806250273
[detection of neutralizing antibodies against avian adenoviruses by a mircotiter cell culture system]. 19806250531
populations of infectious virus produced during avian adenovirus-associated virus infection.multiple rounds of infection in vitro or in vivo with avian adenovirus-associated virus (ava-av) and avian adenovirus (avav) result in production of both heavy (h) and light (l) infectious forms. in this study, the infectious ava-av progeny produced at different hours during recombined dual infection of cells with either h or l ava-av and avav was determined by equilibrium cscl centrifugation and infectivity assay. in both types of infection, h virions were found early, both intra- and extracell ...19806250991
egg transmission of egg drop syndrome 1976 virus in fowl.egg transmission of egg drop syndrome 1976 virus (bc14 virus) in fowl was demonstrated in the second and third week after experimental infection. eggs of bc14 virus infected hens were incubated weekly after disinfection with formaline gas. after 18 days of incubation, eggs with live embryos were homogenized. this egg material was fed to adult hens, housed in isolators. seroconversion in these birds demonstrated egg transmission. it is suggested that egg transmission occurs as a result of viremia ...19806252649
the molecular weight of the egg drop syndrome (eds) avian adenovirus (strain b8/78) dna estimated by digestion with restriction endonuclease enzyme r x ecori. brief report.the restriction endonuclease r x ecori cleaved at two sites of the eds adenovirus (strain b8/78) dna generating 3 fragments with molecular weights of 13.5 x 10(6), 5.0 x 10(6) and 4.4 x 10(6) daltons, respectively. the estimated molecular weight of the whole undigested dna is about 22.9 x 10(6) daltons.19806252866
enhancement and inhibition of celo virus pathogenicity in quail by avian adenovirus-associated virus.dual infection of 12 day-old quail (colinus virginianus) with 10(6) plaque forming units of celo virus and low doses of avian adeno-associated virus (a-av), resulted in significant enhancement of celo virus-induced mortality, whereas dual infections with high doses of a-av resulted in a delay in mortality. a-av induced enhancement and inhibition of celo virus pathogenicity could be blocked by the addition of a-av antiserum prior to infection.19806253685
the oncogenicity of avian adenoviruses. i. an unusually large number of viral dna molecules in some tumors, and virus-specific t-antigenic proteins. 19806254256
the oncogenicity of avian adenoviruses. ii. the arrangement of the viral dna in tumors and transformed cells. 19806254257
an enzyme-linked immunosorbent assay for detection of antibodies to avian adenovirus and avian adenovirus-associated virus in chickens.an enzyme-linked immunosorbent assay system (elisa) was adapted for the detection of antibodies to avian adenovirus (av) and avian adenovirus-associated virus (a-av). both before and after exposure, sera from chickens undergoing natural and experimental infections were assayed by elisa, virus neutralization (vn), and immunodiffusion (id) for antibody to both celo virus and a-av. the elisa system was found to be comparable to vn for determining antibody concentrations to celo virus and a-av. in m ...19806254491
adult coturnix quail bronchitis.quail bronchitis was confirmed by recovery of fowl adenovirus serotype 1 from 2 flocks of adult birds (coturnix coturnix japonica) that were reared commercially for their eggs in the republic of singapore. both flocks were reported to have a fall in egg numbers of 10-15%. the main clinical signs were respiratory distress and soft-shelled and white eggs lacking shell pigmentation.19806254496
serologic detection of adenovirus infections in specific-pathogen-free chickens.a specific-pathogen-free flock of white leghorns, which were housed conventionally and were previously serologically negative for all common poultry pathogens including avian adenoviruses, incurred an outbreak of adenovirus that was detected at about 39 weeks of age. the infection was detected serologically through the agar-gel precipitin test (agpt) and also by a microneutralization test (mnt) adapted for 11 serotypes of avian adenovirus. the mnt detected specific antibodies to serotype-3 avian ...19806254497
isolation of infectious bronchitis, infectious bursal disease and avian adenoviruses from chickens with nephritis-nephrosis syndrome. 19806255706
incidence and distribution of "avian adenovirus group ii splenomegaly of chickens".forty-six percent of 3,585 serum samples from 174 broiler-breeder chicken flocks in 9 states were positive for the precipitating antibody of hemorrhagic enteritis (he) of turkeys/marble spleen disease (msd) of pheasants. seroconversion was detected only in flocks 19 weeks of age and older. the percent of individual serum samples positive within a flock increased with the age of the flock.19806255924
[significance of avian adeno-associated virus (aaav) in serological studies of adenovirus infections in chickens]. 19806258360
[antibody formation after the experimental infection of dogs with various avian adenovirus strains]. 19806260078
neutralizing antibodies to celo and avian adenovirus-associated viruses in the albumen of chicken eggs.neutralizing antibodies to celo virus and to avian adenovirus-associated virus (a-av) were detected in the albumen of eggs from four hens inoculated with these viruses. the antibody concentrations of serum, yolk, and albumen were determined before inoculation and at various times postinoculation (pi) by enzyme-linked immunosorbent assay (elisa) and virus-neutralization (vn) tests. the antibody concentration in albumen was 0.3% to 1.0% of that detected in serum and yolk. uninoculated hens showed ...19806268041
serological identification of avian adenoviruses isolated from cases of inclusion body hepatitis in victoria, australia.sixteen avian adenoviruses isolated from 12 cases of inclusion body hepatitis (ibh), 3 cases of respiratory disease, and a case of ruptured tendons were compared using antisera raised against 9 fowl adenovirus prototype strains. eleven isolates from livers of birds with ibh were classified into 4 different serological groups: 1) yr36 (type 7)-related; 2) hvi (type 8)-related; 3) variants--type 6-,7-, and s-related; and 4) type 50--not closely related to any of the prototype antisera tested. thes ...19806268046
adenovirus-associated virus-precipitating antibody as an early indicator of an adenovirus infection. 1979223478
susceptibility of ducks to the ote strain (celo) of avian adenovirus. 1979225595
transmission of acute respiratory disease (rhinotracheitis) of turkeys.clinical signs of acute respiratory disease of turkeys were transmitted to susceptible day-old poults by direct contact, litter contact, and drinking water. attempts failed to transmit the disease by air (cage-to-cage) or by oral and nasal inoculation with feces, nasal exudates, or nasal turbinate extracts. the disease was not transmitted by inoculation with white blood cells. chicks were not affected by the disease, but young quail developed signs of respiratory disease when exposed to contamin ...1979226051
[distribution of adenovirus in the organ system of chickens (preliminary report)]. 1979226060
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