Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
two ovine mitochondrial dnas harboring a fifth 75/76 bp repeat motif without altered gene expression in northern spain.the basque country is home to the latxa sheep breed, which is divided in several varieties such as latxa black face (lbkf) and latxa blonde face (lblf). mitochondrial dna control region analysis of 174 male sheep (97 lbkf and 77 lblf) was performed with the objective of characterizing the maternal lineages of these two varieties that are the basis to produce the cheese with idiazabal quality label. the percentage of unique haplotypes was 77.32% in lbkf and 67.53% in lblf. most of the individuals ...201727990652
detecting signatures of selection within the tibetan sheep mitochondrial genome.tibetan sheep, a chinese indigenous breed, are mainly distributed in plateau and mountain-valley areas at a terrestrial elevation between 2260 and 4100 m. the herd is genetically distinct from the other domestic sheep and undergoes acclimatization to adapt to the hypoxic environment. to date, whether the mitochondrial dna modification of tibetan sheep shares the same feature as the other domestic breed remains unknown. in this study, we compared the whole mitogenome sequences from 32 tibetan she ...201727937004
characterization of the complete mitochondrial genome sequence of ujumuqin sheep (ovis aries).sheep is one of the most important and widespread domestic animals. nevertheless, the origins of most sheep breeds are poorly understood, specifically the fat-tailed sheep that maternally originates from a specific group. here, we reported the whole mitochondrial dna of ujumuqin sheep (ovis aries), derived from one of the oldest fat-tailed sheep breed-mongolian sheep in china. it harbors 2 ribosomal rna genes, 13 protein-coding genes, 22 transfer rna genes, and a d-loop region. the mitogenome is ...201726713817
accounting for female space sharing in st. kilda soay sheep (ovis aries) results in little change in heritability estimates.when estimating heritability in free-living populations, it is common practice to account for common environment effects, because of their potential to generate phenotypic covariance among relatives thereby biasing heritability estimates. in quantitative genetic studies of natural populations, however, philopatry, which results in relatives being clustered in space, is rarely accounted for. the two studies that have been carried out so far suggest absolute declines in heritability estimates of u ...201727747954
meta-analysis evidence of maternal lineages in chinese tibetan sheep using mtdna d-loop panel.the tibetan sheep is an indigenous breed living in the entire tibetan plateau, and its origin and phylogenic relationships are still uncertain and controversial. in this study, we analyzed partial mtdna d-loop sequences of 156 chinese tibetan sheep individuals from 12 distributed geographic ecotype populations. phylogenetic analysis indicated that three maternal lineages (haplogroups a, b and c) were found in this breed and that ovis vignei and ovis ammon have possibly contributed to the origina ...201727159711
biallelic β-carotene oxygenase 2 knockout results in yellow fat in sheep via crispr/cas9.the recently emerged crispr/cas9 approach represents an efficient and versatile genome editing tool for producing genetically modified animals. β-carotene oxygenase 2 (bco2) is a key enzyme in the progress of β-carotene metabolism and is associated with yellow adipose tissue color in sheep. we have recently demonstrated targeted multiplex mutagenesis in sheep and have generated a group of bco2-disrupted sheep by zygote injection of the crispr/cas9 components. here, we show that biallelic modific ...201727862083
valence of facial cues influences sheep learning in a visual discrimination task.sheep are one of the most studied farm species in terms of their ability to process information from faces, but little is known about their face-based emotion recognition abilities. we investigated (a) whether sheep could use images of sheep faces taken in situation of varying valence as cues in a simultaneous discrimination task and (b) whether the valence of the situation affects their learning performance. to accomplish this, we photographed faces of sheep in three situations inducing emotion ...201729164143
new insights into mitogenomic phylogeny and copy number in eight indigenous sheep populations based on the atp synthase and cytochrome c oxidase genes.the origins and phylogeny of different sheep breeds has been widely studied using polymorphisms within the mitochondrial hypervariable region. however, little is known about the mitochondrial dna (mtdna) content and phylogeny based on mtdna protein-coding genes. in this study, we assessed the phylogeny and copy number of the mtdna in eight indigenous (population size, n=184) and three introduced (n=66) sheep breeds in china based on five mitochondrial coding genes (cox1, cox2, atp8, atp6 and cox ...201729143714
genome-wide scan for runs of homozygosity identifies potential candidate genes associated with local adaptation in valle del belice sheep.because very large numbers of single nucleotide polymorphisms (snps) are now available throughout the genome, they are particularly suitable for the detection of genomic regions where a reduction in heterozygosity has occurred and they offer new opportunities to improve the accuracy of inbreeding ([formula: see text]) estimates. runs of homozygosity (roh) are contiguous lengths of homozygous segments of the genome where the two haplotypes inherited from the parents are identical. here, we invest ...201729137622
draft genome of the marco polo sheep (ovis ammon polii).the marco polo sheep (ovis ammon polii), a subspecies of argali (ovis ammon) that is distributed mainly in the pamir mountains, provides a mammalian model to study high altitude adaptation mechanisms. due to over-hunting and subsistence poaching, as well as competition with livestock and habitat loss, o. ammon has been categorized as an endangered species on several lists. it can have fertile offspring with sheep. hence, a high-quality reference genome of the marco polo sheep will be very helpfu ...201729112761
short communication: milk production and lamb development in saint croix and katahdin hair sheep breeds (ovis aries).the aim of the present study was to characterize milk production and preweaning development of lambs from katahdin and saint croix sheep. milk production was measured weekly, from birth to 8 weeks using oxytocin technique, in 12 katahdin (62.0 ± 5.0 kg) and 11 saint croix (46.2 ± 4.83 kg) multiparous ewes. the mean daily milk production for the katahdin ewes was 1.38 ± 0.54 l/day and for the saint croix ewes it was 1.26 ± 0.49 l/day (p > 0.05) (mean ± se for each group). however, more colostrum ...201729079944
expected net benefit of vaccinating rangeland sheep against bluetongue virus using a modified-live versus killed virus vaccine.recurring outbreaks of bluetongue virus in domestic sheep of the us intermountain west have prompted questions about the economic benefits and costs of vaccinating individual flocks against bluetongue (bt) disease. we estimate the cost of a bt outbreak on a representative rangeland sheep operation in the big horn basin of the state of wyoming using enterprise budgets and stochastic simulation. the latter accounts for variability in disease severity and lamb price, as well as uncertainty about wh ...201729075635
spread of domestic animals across neolithic western anatolia: new zooarchaeological evidence from uğurlu höyük, the island of gökçeada, turkey.the zooarchaeological research presented here investigates neolithic and chalcolithic (ca. 6500-5000 cal. bc) animal exploitation strategies at uğurlu höyük on the turkish island of gökçeada in the northeastern aegean sea. toward this end, we first discuss the results of our analysis of the zooarchaeological assemblages from uğurlu höyük and then consider the data within a wider regional explanatory framework using a diachronic approach, comparing them with those from western and northwestern an ...201729045476
long-term dynamics of mycoplasma conjunctivae at the wildlife-livestock interface in the pyrenees.functional roles of domestic and wild host populations in infectious keratoconjunctivitis (ikc) epidemiology have been extensively discussed claiming a domestic reservoir for the more susceptible wild hosts, however, based on limited data. with the aim to better assess ikc epidemiology in complex host-pathogen alpine systems, the long-term infectious dynamics and molecular epidemiology of mycoplasma conjunctivae was investigated in all host populations from six study areas in the pyrenees and on ...201729016676
nutritional and metabolic stressors on ovine oocyte development and granulosa cell functions in vitro.the present study was undertaken to study the effect of ammonia, urea, non-esterified fatty acid (nefa), and β-hydroxybutyric acid (β-ohb) on oocyte development and granulosa cell (gc) growth parameter of ovine (ovis aries). ovine oocytes were matured in vitro in the presence of different concentration of ammonia, urea, nefa, and β-ohb for 24 h, in vitro inseminated and evaluated for cleavage and blastocyst yield. same concentrations of ammonia, urea, nefa, and β-ohb were examined on growth para ...201728986745
variation in recombination rate and its genetic determinism in sheep populations.recombination is a complex biological process that results from a cascade of multiple events during meiosis. understanding the genetic determinism of recombination can help to understand if and how these events are interacting. to tackle this question, we studied the patterns of recombination in sheep, using multiple approaches and data sets. we constructed male recombination maps in a dairy breed from the south of france (the lacaune breed) at a fine scale by combining meiotic recombination rat ...201728978774
association of heat stress protein 90 and 70 gene polymorphism with adaptability traits in indian sheep (ovis aries).heat stress proteins assist cellular proteins in the acquisition of native structure. the present research was conducted to study how thermo-tolerance is modulated by hsp90 and hsp70 gene polymorphism and its association with hemato-physio-biochemical parameters, supported by their expression profiles in chokla, magra, marwari, and madras red sheep breeds. least square analysis revealed significant effect (p < 0.05) of season and breed on all the physiological parameters, i.e., temperature, resp ...201728265807
[primary malignant melanoma in the brain of a 7-month-old sheep (ovis aries f. domestica)].a case of malignant melanoma in a sheep's brain is described for the first time. in a 7-month-old sheep that had been euthanized due to ataxia, post-mortem and histopathologic examinations were performed. both the brain and the calvarium were heavily infiltrated with neoplastic tissue. metastases were found in the liver and kidneys. histomorphology confirmed the gross pathologic impression of malignancy. congenital melanosis, which is regularly present in the meninx of sheep, could have been the ...201728197623
calm merino ewes have a higher ovulation rate and more multiple pregnancies than nervous ewes.in 1990, two selection lines of merino sheep were established for low and high behavioural reactivity (calm and nervous temperament) at the university of western australia. breeding records consistently showed that calm ewes weaned 10% to 19% more lambs than the nervous ewes. we hypothesise that calm ewes could have a higher ovulation rate than nervous ewes and/or calm ewes could have a lower rate of embryo mortality than nervous ewes. we tested these hypotheses by comparing the ovulation rate a ...201728183365
mitochondrial dna sequence analysis of the mexican creole sheep (ovis aries) reveals a narrow iberian maternal origin.the creole sheep in america is supposed to have originated specifically from the iberian peninsula and introduced by the spaniards during the colonization. however, it is not clear their genetic relationship with iberian breeds. the genetic origin and diversity of the mexican creole sheep (mcs) were investigated by mitochondrial dna control region nucleotide sequences. dna sequence from 33 mcs samples from three regions of méxico revealed 21 different haplotypes. phylogenetic analysis including ...201728141972
expression of adenosine 5'-monophosphate-activated protein kinase (ampk) in ovine testis (ovis aries): in vivo regulation by nutritional state.in the present study, we identified ampk and investigated its potential role in steroidogenesis in vivo in the ovine testis in response to variation in nutritional status (fed control vs. restricted). we performed immunoblotting to show that both active and non-active forms of ampk exist in ovine testis and liver. in testis, we confirmed these results by immunohistochemistry. we found a correlation between atp (adenosine-triphosphate) levels and the expression of ampk in liver. also, low and hig ...201728122665
joint associations of blood plasma proteins with overwinter survival of a large mammal.in many wild animal populations, hosts are at risk of parasites and malnutrition and resource costs of defence may be difficult to afford. we postulate that proteins, important in homeostasis and immunity, play a complex but central role in condition dependence and resource costs of mammalian immune defence. to test this, we measured plasma concentrations of albumin, total proteins. self-reactive antibodies and parasite-specific igg in female soay sheep. using a principal component analysis, we ...201728111903
genetic diversity of haemonchus contortus isolated from sympatric wild blue sheep (pseudois nayaur) and sheep in helan mountains, china.haemonchus contortus is known among parasitic nematodes as one of the major veterinary pathogens of small ruminants and results in great economic losses worldwide. human activities, such as the sympatric grazing of wild with domestic animals, may place susceptible wildlife hosts at risk of increased prevalence and infection intensity with this common small ruminant parasite. studies on phylogenetic factors of h. contortus should assist in defining the amount of the impact of anthropogenic factor ...201728927469
a high resolution atlas of gene expression in the domestic sheep (ovis aries).sheep are a key source of meat, milk and fibre for the global livestock sector, and an important biomedical model. global analysis of gene expression across multiple tissues has aided genome annotation and supported functional annotation of mammalian genes. we present a large-scale rna-seq dataset representing all the major organ systems from adult sheep and from several juvenile, neonatal and prenatal developmental time points. the ovis aries reference genome (oar v3.1) includes 27,504 genes (2 ...201728915238
pharmacokinetics of a transdermal fentanyl solution in suffolk sheep (ovis aries).sheep used as surgical models require appropriate pain management, and the commonly used transdermal fentanyl patches require a long predosing period to achieve adequate plasma concentrations. the aim of this study was to assess the pharmacokinetic parameters of an fda-approved transdermal fentanyl solution (tfs) that has yet to be tested in sheep. in this study, we compared tfs at 2.7 mg/kg (n = 2), 1.7 mg/kg (n = 3), and 0.5 mg/kg (n = 3) with the control fentanyl patch at 2 μg/kg/h (n = 1); b ...201728903827
alteration of sheep coat color pattern by disruption of asip gene via crispr cas9.coat color is an important characteristic and economic trait in domestic sheep. aiming at alteration of chinese merino sheep coat color by genome manipulation, we disrupted sheep agouti signaling protein gene by crispr/cas9. a total of seven indels were identified in 5 of 6 born lambs. each targeted lamb happened at least two kinds of modifications, and targeted lambs with multiple modifications displayed variety of coat color patterns. three lambs with 4 bp deletion showed badgerface with black ...201728811591
robust extraction of quantitative structural information from high-variance histological images of livers from necropsied soay sheep.quantitative information is essential to the empirical analysis of biological systems. in many such systems, spatial relations between anatomical structures is of interest, making imaging a valuable data acquisition tool. however, image data can be difficult to analyse quantitatively. many image processing algorithms are highly sensitive to variations in the image, limiting their current application to fields where sample and image quality may be very high. here, we develop robust image processi ...201728791138
genomic signatures of adaptive introgression from european mouflon into domestic sheep.mouflon (ovis aries musimon) became extinct from mainland europe after the neolithic, but remnant populations from the mediterranean islands of corsica and sardinia have been used for reintroductions across europe since the 19th-century. mouflon x sheep hybrids are larger-bodied than mouflon, potentially showing increased male reproductive success, but little is known about genomic levels of admixture, or about the adaptive significance of introgression between resident mouflon and local sheep b ...201728790322
uranium and associated heavy metals in ovis aries in a mining impacted area in northwestern new mexico.the objective of this study was to determine uranium (u) and other heavy metal (hm) concentrations (as, cd, pb, mo, and se) in tissue samples collected from sheep (ovis aries), the primary meat staple on the navajo reservation in northwestern new mexico. the study setting was a prime target of u mining, where more than 1100 unreclaimed abandoned u mines and structures remain. the forage and water sources for the sheep in this study were located within 3.2 km of abandoned u mines and structures. ...201728788090
sarcocystis spp. in domestic sheep in kunming city, china: prevalence, morphology, and molecular characteristics.sheep (ovis aries) are intermediate hosts for at least six named species of sarcocystis: s. tenella, s. arieticanis, s. gigantea, s. medusiformis, s. mihoensis, and s. microps. here, only two species, s. tenella and s. arieticanis, were found in 79 of 86 sheep (91.9%) in kunming, china, based on their morphological characteristics. four genetic markers, i.e., 18s rrna gene, 28s rrna gene, mitochondrial cox1 gene, and its-1 region, were sequenced and characterized for the two species of sarcocyst ...201728766501
genetic polymorphism of growth differentiation factor 9 (gdf9) gene related to fecundity in two egyptian sheep breeds.this study explores polymorphisms in the growth differentiation factor 9 (gdf9) gene (exon 1) with respect to fertility in egyptian sheep.201728762037
physiological proteins in resource-limited herbivores experiencing a population die-off.nutrient availability is predicted to interact with herbivore population densities. competition for low quality food at high density may reduce summer food intake, and in turn winter survival. conversely, low population density may favor physiological recovery through better access to better quality spring forage. here, we take advantage of the long-term study of the soay sheep population of st. kilda (scotland) to measure plasma protein markers and immunity in two consecutive summers with contr ...201728761976
underdominant kcc3b r31i association with blood sodium concentration in domestic sheep suggests role in oligomer function. 201728748545
plant community chemical composition influences trembling aspen (populus tremuloides) intake by sheep.nutrients and plant secondary compounds in aspen (populus tremuloides) may interact with nutrients in the surrounding vegetation to influence aspen use by herbivores. thus, this study aimed to determine aspen intake and preference by sheep in response to supplementary nutrients or plant secondary compounds (psc) present in aspen trees. thirty-two lambs were randomly assigned to one of four molasses-based supplementary feeds to a basal diet of tall fescue hay (n = 8) during three experiments. the ...201728744731
estimation of linkage disequilibrium and effective population size in new zealand sheep using three different methods to create genetic maps.investments in genetic selection have played a major role in the new zealand sheep industry competitiveness. selection may erode genetic diversity, which is a crucial factor for the success of breeding programs. better understanding of linkage disequilibrium (ld) and ancestral effective population size (ne) through quantifying this diversity and comparison between populations allows for more informed decisions with regards to selective breeding taking population genetic diversity into account. t ...201728732466
alzheimer's disease markers in the aged sheep (ovis aries).this study reports the identification and characterization of markers of alzheimer's disease (ad) in aged sheep (ovis aries) as a preliminary step toward making a genetically modified large animal model of ad. importantly, the sequences of key proteins involved in ad pathogenesis are highly conserved between sheep and human. the processing of the amyloid-β (aβ) protein is conserved between sheep and human, and sheep aβ1-42/aβ1-40ratios in cerebrospinal fluid (csf) are also very similar to human. ...201728728117
understanding the inguinal sinus in sheep (ovis aries)-morphology, secretion, and expression of progesterone, estrogens, and prolactin receptors.post-parturient behavior of mammalian females is essential for early parent-offspring contact. after delivery, lambs need to ingest colostrum for obtaining the related immunological protection, and early interactions between the mother and the lamb are crucial. despite visual and auditory cues, olfactory cues are decisive in lamb orientation to the mammary gland. in sheep, the inguinal sinus is located bilaterally near the mammary gland as a skin pouch (igs) that presents a gland that secretes a ...201728703772
genome-wide association analysis identifies the genetic basis of fat deposition in the tails of sheep (ovis aries).fat-tailed sheep (ovis aries) can survive in harsh environments and satisfy human's intake of dietary fat. however, the animals require more feed, which increases the cost of farming. thus, most farmers currently prefer thin-tailed, short-tailed or docked sheep. to date, the molecular mechanism of the formation of fat tails in sheep has not been completely elucidated. here, we conducted a genome-wide association study using phenotypes and genotypes (the ovine infinium hd snp beadchip genotype da ...201728677334
occipital condylar dysplasia in a jacob lamb (ovis aries).jacob sheep (ovis aries) are a pedigree breed known for their "polycerate" (multihorned) phenotype. we describe a four-horned jacob lamb that exhibited progressive congenital hindlimb ataxia and paresis, and was euthanased four weeks post-partum. necropsy and ct-scan revealed deformity and asymmetry of the occipital condyles, causing narrowing of the foramen magnum and spinal cord compression. histopathology demonstrated wallerian degeneration of the cervical spinal cord at the level of the fora ...201728652978
genomic reconstruction of the history of native sheep reveals the peopling patterns of nomads and the expansion of early pastoralism in east asia.china has a rich resource of native sheep (ovis aries) breeds associated with historical movements of several nomadic societies. however, the history of sheep and the associated nomadic societies in ancient china remains poorly understood. here, we studied the genomic diversity of chinese sheep using genome-wide snps, mitochondrial and y-chromosomal variations in > 1,000 modern samples. population genomic analyses combined with archeological records and historical ethnic demographics data reveal ...201728645168
scanning electron microscopy-energy-dispersive x-ray (sem/edx): a rapid diagnostic tool to aid the identification of burnt bone and contested cremains.this study investigates the use of scanning electron microscopy-energy-dispersive x-ray (sem-edx) as a diagnostic tool for the determination of the osseous origin of samples subjected to different temperatures. sheep (ovis aries) ribs of two experimental groups (fleshed and defleshed) were burned at temperatures of between 100°c and 1100°c in 100°c increments and subsequently analyzed with the sem-edx to determine the atomic percentage of present elements. three-factor anova analysis showed that ...201728605021
the ovine motor cortex: a review of functional mapping and cytoarchitecture.in recent years, sheep (ovis aries) have emerged as a useful animal model for neurological research due to their relatively large brain and blood vessel size, their cortical architecture, and their docile temperament. however, the functional anatomy of sheep brain is not as well studied as that of non-human primates, rodents, and felines. for example, while the location of the sheep motor cortex has been known for many years, there have been few studies of the somatotopy of the motor cortex and ...201728595827
determination of renal handling of marbofloxacin in lohi sheep (ovis aries) following a single intravenous administration.the objective of present study was to investigate renal clearance, urinary excretion and underlying excretory mechanism of marbofloxacin in lohi sheep. for this purpose, marbofloxacin was administered intravenously (iv) as single bolus dose (2.5 mg/kg body weight) to eight healthy sheep of lohi breed. after start of experiment, blood and urine samples were drawn at predetermined time intervals and marbofloxacin concentrations in the samples were measured by reverse phase high performance liquid ...201728588633
so sheep may safely graze: honing in on culprit behind fleece variation in domesticated sheep. 201728482052
erratum to: association of heat stress protein 90 and 70 gene polymorphism with adaptability traits in indian sheep (ovis aries). 201728470623
genome-wide identification of the mutation underlying fleece variation and discriminating ancestral hairy species from modern woolly sheep.the composition and structure of fleece variation observed in mammals is a consequence of a strong selective pressure for fiber production after domestication. in sheep, fleece variation discriminates ancestral species carrying a long and hairy fleece from modern domestic sheep (ovis aries) owning a short and woolly fleece. here, we report that the "woolly" allele results from the insertion of an antisense eif2s2 retrogene (called aseif2s2) into the 3' utr of the irf2bp2 gene leading to an abnor ...201728379502
the sheep (ovis aries) muscle proteome: decoding the mechanisms of tolerance to seasonal weight loss using label-free proteomics.seasonal weight loss (swl) is one of the most pressing issues in animal production in the tropics and mediterranean. this work aims to characterize muscle proteome changes as a consequence of swl in meat producing sheep, using a label-free proteomics approach. we compare three breeds: the australian merino (swl susceptible), the damara (swl tolerant) and the dorper (swl intermediate tolerance). we identified 668 proteins of the sheep proteome, 95 with differential regulation. also we observe tha ...201728366878
provision of physiological data and reference values in awake and anaesthetized female sheep aged 6-12 months.to provide physiological data and reference values in awake and anaesthetized sheep aged 6-12 months.201728330726
prospective transfrontal sheep model of skull-base reconstruction using vascularized mucosa.no high-fidelity animal model exists to examine prospective wound healing following vascularized reconstruction of the skull base. such a model would require the ability to study the prospective behavior of vascularized mucosal repairs of large dural and arachnoid defects within the intranasal environment. the objective of this study was to therefore develop and validate a novel, in vivo, transfrontal sheep model of cranial base repair using vascularized sinonasal mucosa.201729227037
occurrence of tongue worm,linguatula cf. serrata(pentastomida: linguatulidae) in wild canids and livestock in south-eastern australia.pentastomids are obligate zoonotic arthropod parasites utilising canids and vulpids as their definitive hosts and several herbivorous species as their intermediate hosts. reported only 10 times in australia over the last 150 years as incidental findings, adult pentastomids referred to aslinguatula serratahave been encountered in nasal cavities of domestic and wild dogs, and foxes. nymphs have been reported in cattle and rabbits. in the present study, a number of potential definitive hosts, inclu ...201728971014
corrigendum: bacterial community composition and fermentation in the rumen of xinjiang brown cattle (bos taurus), tarim red deer (cervus elaphus yarkandensis), and karakul sheep (ovis aries). 201728925282
interlaboratory validation of a real-time pcr detection method for bovine- and ovine-derived material.in this work we performed interlaboratory validation of a taqman real-time pcr method for the identification of bovine and ovine material. the bos taurus beta-actin gene (actb) and ovis aries prolactin receptor gene (prlr) were selected as the bovine and ovine species-specific amplifying target sequences, and primers and taqman probes were designed accordingly. the precision, efficiency, false positive rate, limit of detection (lod95%) and probability of detection (pod) were determined, and the ...201728780135
proteomic analysis of seminal plasma from locally-adapted "curraleiro pé-duro bulls" (bos taurus): identifying biomarkers involved in sperm physiology in endangered animals for conservation of biodiversity.the present study was aimed at evaluating the seminal plasma proteins and sperm parameters of curraleiro pé-duro bulls. semen was collected from 10 bulls by electroejaculation, and sperm parameters were evaluated in fresh and frozen-thawed semen. seminal plasma proteins were analyzed by 2-d sds-page and mass spectrophotometry. tools in computational biology were used to generate bioinformatic knowledge and evaluate gene ontology, protein-protein interactions, phylogenetic trees and multiple sequ ...201728625714
sheep recognize familiar and unfamiliar human faces from two-dimensional images.one of the most important human social skills is the ability to recognize faces. humans recognize familiar faces easily, and can learn to identify unfamiliar faces from repeatedly presented images. sheep are social animals that can recognize other sheep as well as familiar humans. little is known, however, about their holistic face-processing abilities. in this study, we trained eight sheep (ovis aries) to recognize the faces of four celebrities from photographic portraits displayed on computer ...201729291109
functional role of cyclin-dependent kinase 5 in the regulation of melanogenesis and epidermal structure.the mammalian integumentary system plays important roles in body homeostasis, and dysfunction of melanogenesis or epidermal development may lead to a variety of skin diseases, including melanoma. skin pigmentation in humans and coat color in fleece-producing animals are regulated by many genes. among them, microphthalmia-associated transcription factor (mitf) and paired-box 3 (pax3) are at the top of the cascade and regulate activities of many important melanogenic enzymes. here, we report for t ...201729062096
developmental morphology and topography of the components of the cervical sympathetic trunk in sheep (ovis aries) during the fetal period.the objective of this study was to clarify the typical architecture and morphological variations of cervical sympathetic trunk (cst) in sheep during fetal period. components of cst were examined on both sides of 40 male and female sheep fetuses aged from 60 to 140 days under a stereomicroscope. skeletotopy and frequency of presence of cranial cervical ganglion (ccg), syntopy of cervical ganglia, and composition and topography of vagosympathetic trunk were consistent among specimens whereas the s ...201728865185
a genome-wide association study reveals candidate genes for the supernumerary nipple phenotype in sheep (ovis aries).genome-wide association studies (gwass) have been widely applied in livestock to identify genes associated with traits of economic interest. here, we conducted the first gwas of the supernumerary nipple phenotype in wadi sheep, a native chinese sheep breed, based on ovine infinium hd snp beadchip genotypes in a total of 144 ewes (75 cases with four teats, including two normal and two supernumerary teats, and 69 control cases with two teats). we detected 63 significant snps at the chromosome-wise ...201728703336
gastrointestinal nematodes and the deworming of mouflon (ovis aries musimon) from goleniowska forest in west pomerania province, polandruminants often live in environments where the natural balance has been disturbed by humans. as a result, there is a transfer of parasitosis to domestic animals and sometimes humans. the aim of the study was to determine the annual species composition and level of gastrointestinal nematode infection of mouflon (ovis aries musimon) from the half-open breeding colonizing areas of the goleniowska forest, and to assess the effectiveness of deworming. the course of the parasitic infection of mouflon ...201728574237
mitochondrial dna analysis of ancient sheep from altai.a comparative analysis of the genetic diversity of ancient and modern sheep can shed light on the origin of these animals and their distribution as well as help to evaluate the role of humans at each formation stage of different sheep breeds. here we isolated ancient dna and performed sequencing of the mitochondrial dna d-loop from 17 sheep bone remains (~4000-1000 years old) found in the archaeological complexes in the south of altai (western siberia). the length of the sequences obtained range ...201728568904
ovis aries papillomavirus 3 in ovine cutaneous squamous cell carcinoma.squamous cell carcinoma (scc) is a common malignancy affecting humans and other animals. papillomaviruses (pvs) are frequently reported as causal agents of cutaneous benign and malignant epithelial lesions in different animal species, but only few studies have investigated their role in ovine scc. in this study, we explore the possible involvement of the ovine aries pvs (oapv1, oapv2, oapv3) in cutaneous scc using an integrated histological and molecular approach. forty cutaneous sccs from diffe ...201728494708
a metagenomics investigation of carbohydrate-active enzymes along the gastrointestinal tract of saudi sheep.the digestive microbiota of humans and of a wide range of animals has recently become amenable to in-depth studies due to the emergence of dna-based metagenomic techniques that do not require cultivation of gut microbes. these techniques are now commonly used to explore the feces of humans and animals under the assumption that such samples are faithful proxies for the intestinal microbiota. sheep (ovis aries) are ruminant animals particularly adapted to life in arid regions and in particular naj ...201728473812
sex differences in leucocyte telomere length in a free-living mammal.mounting evidence suggests that average telomere length reflects previous stress and predicts subsequent survival across vertebrate species. in humans, leucocyte telomere length (ltl) is consistently shorter during adulthood in males than in females, although the causes of this sex difference and its generality to other mammals remain unknown. here, we measured ltl in a cross-sectional sample of free-living soay sheep and found shorter telomeres in males than in females in later adulthood (>3 ye ...201728027420
cytochrome b conservation between six camel breeds reared in egypt.this study was aimed to assess cytochrome b conservation in six breeds of camels reared in egypt and to compare its sequence with those of other livestock species. the 208-bp fragments from camel mtdna cyto b were amplified using pcr for 54 camels belonging to 6 camel breeds reared in egypt. the alignment of camel cyto b sequences showed the presence of two polymorphic sites resulting in four haplotypes and their nucleotide sequences were submitted to genbank under the accession numbers: kx90989 ...201730647635
infection levels of protostrongylid nematodes in definitive caprine and intermediate gastropod hosts from uzbekistan.morphological analysis of lungworms collected among caprinae from uzbekistan resulted in the identification of four species of protostrongylidae: protostrongylus rufescens, protostrongylus hobmaieri, spiculocaulus leuckarti and cystocaulus ocreatus. the following species were recorded as definitive hosts: ovis aries, ovis ammon, ovis vignei, capra hircus, capra falconeri and capra sibirica. the prevalence of p. rufescens reached 45.3%, followed by s. leuckarti and c. ocreatus with 31.7% and p. h ...201727018914
corynebacterium pseudotuberculosis infection in patagonian huemul ( hippocamelus bisulcus ).corynebacterium pseudotuberculosis is an intracellular bacteria and the etiologic agent of caseous lymphadenitis in domestic and wildlife species. we report c. pseudotuberculosis infection in patagonian huemul ( hippocamelus bisulcus ) from the cerro castillo national reserve, region of aysen, chile. subcutaneous abscesses in the abdominal and pectoral regions from two animals were sampled and bacteriologic isolation was performed. in both cases, we isolated a c. pseudotuberculosis strain belong ...201728323562
wide exposure to coxiella burnetii in ruminant and feline species living in a natural environment: zoonoses in a human-livestock-wildlife interface.assessment of the role of wild and domestic hosts as potential reservoirs of misdiagnosed zoonoses, such as q fever by coxiella burnetii, is an important public health issue today both for wildlife conservation and management of disease in human-livestock-wildlife interface. this study used elisa, an indirect antibody, to research (2003-2013) c. burnetii infection in seven free-living wild and domestic ruminant species and in european wildcats (felis silvestris). the animals studied were 0 europ ...201727776577
differential susceptibility of bighorn sheep (ovis canadensis) and domestic sheep (ovis aries) neutrophils to mannheimia haemolytica leukotoxin is not due to differential expression of cell surface cd18.bighorn sheep ( ovis canadensis ) are more susceptible to pneumonia caused by mannheimia haemolytica than are domestic sheep ( ovis aries ). leukotoxin produced by m. haemolytica is the principal virulence factor involved in pneumonia pathogenesis. although leukotoxin is cytolytic to all subsets of ruminant leukocytes, neutrophils are the most susceptible subset. bighorn sheep neutrophils are 4- to 8-fold more susceptible to leukotoxin-induced cytolysis than are domestic sheep neutrophils. we hy ...201728323564
infectious keratoconjunctivitis in wild caprinae: merging field observations and molecular analyses sheds light on factors shaping outbreak dynamics.infectious keratoconjunctivitis (ikc) is an ocular infectious disease caused by mycoplasma conjunctivae which affects small domestic and wild mountain ruminants. domestic sheep maintain the pathogen but the detection of healthy carriers in wildlife has raised the question as to whether m. conjunctivae may also persist in the wild. furthermore, the factors shaping the dynamics of ikc outbreaks in wildlife have remained largely unknown. the aims of this study were (1) to verify the etiological rol ...201728259139
a novel astrovirus associated with encephalitis and ganglionitis in domestic sheep.in june 2013, a 4-year-old welsh mountain ewe and in march 2014 a 10-day-old lamb of the same breed and the same flock presented progressive neurological signs including depressed sensorium, tremor, and unusual behaviour. neuropathological examination of the brain and spinal cord detected non-suppurative polioencephalomyelitis and dorsal root ganglionitis, characteristic of a neurotropic viral agent in both sheep. metagenomic analysis of different tissue samples from both animals identified a no ...201728224712
diet, dietary selectivity and density of south american grey fox, lycalopex griseus, in central chile.the south american grey fox (lycalopex griseus) is a canid widely distributed in southern south america, however some aspects of its biology are still poorly known. we studied the diet and density of l. griseus in the lago peñuelas biosphere reserve, in central chile. the trophic niche breadth was b = 6.16 (bsta = 0.47) and prey diversity was h ' = 2.46 (hmax' = 3.17, j' = 0.78). the highest proportions of prey consumed in the diet were oryctolagus cuniculus (52.21%) and other mammals (32.78%). ...201728262006
comparative genomic identification and validation of β-defensin genes in the ovis aries genome.β-defensins are small, cationic, antimicrobial peptides found in species across the plant and animal kingdoms. in addition to microbiocidal activity, roles in immunity as well as reproduction have more recently been documented. β-defensin genes in ovis aries (domestic sheep) have been poorly annotated, having been identified only by automatic gene prediction algorithms. the objective of this study was to use a comparative genomics approach to identify and characterise the β-defensin gene reperto ...201728376793
an aanat/asmt transgenic animal model constructed with crispr/cas9 system serving as the mammary gland bioreactor to produce melatonin-enriched milk in sheep.melatonin as a potent antioxidant exhibits important nutritional and medicinal values. to produce melatonin-enriched milk will benefit the consumers. in this study, a sheep bioreactor which generates melatonin-enriched milk has been successfully developed by the technology that combined crispr/cas9 system and microinjection. the aanat and asmt were cloned from pineal gland of dorper sheep (ovis aries). the in vitro studies found that aanat and asmt were successfully transferred to the mammary ep ...201728273380
bacterial community composition and fermentation in the rumen of xinjiang brown cattle (bos taurus), tarim red deer (cervus elaphus yarkandensis), and karakul sheep (ovis aries).the rumen microbiota plays a major role in the metabolism and absorption of indigestible food sources. xinjiang brown cattle (bos taurus), tarim red deer (cervus elaphus yarkandensis), and karakul sheep (ovis aries) are important ruminant species for animal husbandry in the tarim basin. however, the microbiota and rumen fermentation of these animals are poorly understood. here, we apply high-throughput sequencing to examine the bacterial community in the rumen of cattle, red deer and sheep and m ...201728177790
a brief and critical review on hydrofluorosis in diverse species of domestic animals in india.india is one of the fluoride-endemic countries where the maximum numbers of ground or drinking water sources are naturally fluoridated. in india, a total of 23, out of 36 states and union territories have drinking water contaminated with fluoride in varying concentration. in the present scenario, especially in rural india, besides the surface waters (perennial ponds, dams, rivers, etc.), bore wells and hand pumps are the principal drinking water sources for domestic animals such as cattle (bos t ...201728144801
molecular characterization of hoxc8 gene and methylation status analysis of its exon 1 associated with the length of cashmere fiber in liaoning cashmere goat.homeobox protein hox-c8 (hoxc8) is a member of hox family. it is expressed in the dermal papilla of the skin and is thought to be associated with the hair inductive capacity of dermal papilla cells. in the present study, we isolated and characterized a full-length open reading frame of hoxc8 cdna from the skin tissue of liaoning cashmere goat, as well as, established a phylogenetic relationship of goat hoxc8 with that of other species. also, we investigated the effect of methylation status of ho ...201728093668
validation of a fast real-time pcr method to detect fraud and mislabeling in milk and dairy products.fast real-time pcr taqman assays were developed and validated for species identification in dairy products. based on the amplification of 12s rrna and cytb partial genes of mitochondrial dna, the methods were demonstrated to be sensitive, fast, and species-specific for bos taurus, ovis aries, bubalus bubalis, and capra hircus. the limit of detection calculated was lower than 1%, and the efficiency was reported to be higher than 96% in every assay. an internal amplification control was used to de ...201727865504
ged: a manually curated comprehensive resource for epigenetic modification of gametogenesis.reproductive infertility affects seventh of couples, which is most attributed to the obstacle of gametogenesis. characterizing the epigenetic modification factors involved in gametogenesis is fundamental to understand the molecular mechanisms and to develop treatments for human infertility. although the genetic factors have been implicated in gametogenesis, no dedicated bioinformatics resource for gametogenesis is available. to elucidate the relationship of epigenetic modification and mammalian ...201726851225
molecular characterisation, genetic variability and detection of a functional polymorphism influencing the promoter activity of oxt gene in goat and sheep.the purpose of the study described in this research communication was to report the full characterisation of the goat and sheep oxytocin-neurophysin i gene (oxt), their promoters and amino acid sequences. using the genomic dna as template, we sequenced and compared the whole oxt gene (3 exons), plus 958/960 nucleotides at the 5' flanking region and 478/477 nucleotides at the 3' flanking region, in 46 sheep and 24 goats belonging to different breeds/genetic types reared in italy, greece and germa ...201728290268
home-made cost effective preservation buffer is a better alternative to commercial preservation methods for microbiome research.the investigation of wildlife gastrointestinal microbiomes by next-generation sequencing approaches is a growing field in microbial ecology and conservation. such studies often face difficulties in sample preservation if neither freezing facilities nor liquid nitrogen (lqn) are readily available. thus, in order to prevent microbial community changes because of bacterial growth after sampling, preservation buffers need to be applied to samples. however, the amount of microbial community variation ...201728197142
species identification in meat products: a new screening method based on high resolution melting analysis of cyt b gene.meat adulteration by substitution with lower value products and/or mislabeling involves economic, health, quality and socio-religious issues. therefore, identification and traceability of meat species has become an important subject to detect possible fraudulent practices. in the present study the development of a high resolution melt (hrm) screening method for the identification of eight common meat species is reported. samples from bos taurus, ovis aries, sus scrofa domestica, equus caballus, ...201728764056
ethnomedicinal and cultural practices of mammals and birds in the vicinity of river chenab, punjab-pakistan.although, use of animal species in disease treatment and culture practices is as ancient as that of plant species; however ethnomedicinal uses and cultural values of animal species have rarely been reported. present study is the first report on the medicinal uses of mammals and bird species in pakistan.201728701165
molecular epidemiological survey and genetic characterization of anaplasma species in mongolian livestock.anaplasma species are obligate intracellular rickettsial pathogens that cause great economic loss to the animal industry. few studies on anaplasma infections in mongolian livestock have been conducted. this study examined the prevalence of anaplasma marginale, anaplasma ovis, anaplasma phagocytophilum, and anaplasma bovis by polymerase chain reaction assay in 928 blood samples collected from native cattle and dairy cattle (bos taurus), yaks (bos grunniens), sheep (ovis aries), and goats (capra a ...201728678004
validation of an improved anaplasma antibody competitive elisa for detection of anaplasma ovis antibody in domestic sheep.an accurate and simple-to-perform new version of a competitive elisa (celisa) kit that became commercially available in 2015 for testing of cattle for antibody to anaplasma marginale was validated for detection of anaplasma ovis antibody in domestic sheep. true positives and negatives were identified using nested pcr (npcr) as the gold standard. negative bovine control sera supplied with the kit were used to calculate % inhibition (%i), designated bovine control elisa (bcelisa), and this was com ...201728480803
effect of vaccination against pneumonia on the survival of bighorn sheep (ovis canadensis) commingled with carrier animals.leukotoxin producing (lkt+) members of pasteurellaceae, particularly mannheimia haemolytica and bibersteinia trehalosi are important pathogens of pneumonia in bighorn sheep (bhs; ovis canadensis), causing fatal disease. predisposing or concurrent infection with mycoplasma ovipneumoniae enhances the severity of the disease, resulting in increased morbidity and mortality. several studies have investigated the effectiveness of vaccines against lkt+ members of pasteurellaceae in preventing fatal pne ...201728619167
description of diploplastron dehorityi sp. nov. (entodiniomorphida, ophryoscolecidae), a new rumen ciliate from brazilian sheep (ovis aries).to date the genus diploplastron comprised only one species of ophryoscolecid ciliate, diploplastron affine, which is characterized by having two retractable ciliary zones in the anterior end of the body, two slender and juxtaposed skeletal plates on the right side, a rod shape macronucleus, and two contractile vacuoles. during study on the characterization of rumen ciliate community composition in brazilian domestic sheep, we observed ciliates with atypical morphology but with diagnostic feature ...201728609901
endogenous retroviral insertions indicate a secondary introduction of domestic sheep lineages to the caucasus and central asia between the bronze and iron age.sheep were one of the first livestock species domesticated by humans. after initial domestication in the middle east they were spread across eurasia. the modern distribution of endogenous jaagsiekte sheep retrovirus insertions in domestic sheep breeds suggests that over the course of millennia, successive introductions of improved lineages and selection for wool quality occurred in the mediterranean region and most of asia. here we present a novel ancient dna approach using data of endogenous re ...201728632161
exposure of bighorn sheep to domestic goats colonized with mycoplasma ovipneumoniae induces sub-lethal pneumonia.bronchopneumonia is a population limiting disease of bighorn sheep (ovis canadensis) that has been associated with contact with domestic caprinae. the disease is polymicrobial but is initiated by mycoplasma ovipneumoniae, which is commonly carried by both domestic sheep (o. aries) and goats (capra aegagrus hircus). however, while previous bighorn sheep comingling studies with domestic sheep have resulted in nearly 100% pneumonia mortality, only sporadic occurrence of fatal pneumonia was reported ...201728591169
reliable molecular differentiation of trichuris ovis and trichuris discolor from sheep (ovis orientalis aries) and roe deer (capreolus capreolus) and morphological characterisation of their females: morphology does not work sufficiently.the main aim of the study was to evaluate associations between morphological variability of trichuris females from sheep and roe deer and their rdna polymorphism in whipworm populations from the czech republic. the results introduced the use of new molecular markers based on the internal transcribed spacer (its)1-5.8s rna-its2 region polymorphisms, as useful tools for the unambiguous differentiation of congeners trichuris ovis and trichuris discolor. these markers revealed both parasites in roe ...201728624874
tick-borne pathogens and their reservoir hosts in northern italy.the aim of this study was to determine the occurrence of anaplasma phagocytophilum, rickettsia spp., babesia spp., and candidatus neoehrlichia mikurensis in ixodes spp. ticks removed from wildlife, domestic animals and humans in the province of trento (northern italy) in order to better understand their ecology and provide public health professionals with an updated list of pathogens which should be considered during their diagnostic procedures after a tick bite. during 2011-2012, 848 feeding ti ...201728890111
first record of gongylonema nepalensis in domestic and wild ruminants in europe.the gullet worm, gongylonema pulchrum, is cosmopolitan in distribution, infecting a variety of mammals including domestic and wild ruminants. gongylonema nepalensis recently collected from the esophageal epithelium of water buffaloes in nepal was separated from g. pulchrum based on its distinctly shorter left spicule relative to body length and unique nucleotide sequences of the ribosomal rna gene (rdna) and the mitochondrial cytochrome c oxidase subunit 1 gene (cox-1). during meat inspections a ...201728969772
analysis of codon usage bias of crimean-congo hemorrhagic fever virus and its adaptation to hosts.crimean-congo hemorrhagic fever virus (cchfv) is a negative-sense, single stranded rna virus with a three-segmented genome that belongs to the genus nairovirus within the family bunyaviridae. cchfv uses hyalomma ticks as a vector to infect humans with a wide range of clinical signs, from asymptomatic to zika-like syndrome. despite significant progress in genomic analyses, the influences of viral relationships with different hosts on overall viral fitness, survival, and evading the host's immune ...201729198972
the effects of acaricide treatment of sheep on red grouse lagopus lagopus scotica tick burdens and productivity in a multi-host system.ixodes ricinus (ixodida: ixodidae) ticks are of economic and pathogenic importance across europe. within the uplands of the u.k., management to reduce ticks is undertaken to benefit red grouse lagopus lagopus scotica (galliformes: phasianidae). management strategies focus on the acaricide treatment of domestic sheep ovis aries (artiodactyla: bovidae), but the effectiveness of this is less certain in the presence of wild hosts, particularly red deer cervus elaphus (artiodactyla: cervidae) and mou ...201729194726
thermoregulation of male sheep of indigenous or exotic breeds in a tropical environment.climate change has intensified the frequency of heat waves in the world, thereby exposing farm animals to stressful conditions. for better productive performance it is important to identify the most resilient genotypes. thus, our objective was to evaluate the thermoregulatory responses of rams of tropical indigenous (morada nova and santa inês) and exotic breeds (dorper and texel), by monitoring the environmental and physiological indicators related to heat tolerance. the experiment was carried ...201729037398
high systemic and testicular thermolytic efficiency during heat tolerance test reflects better semen quality in rams of tropical breeds.this study aimed to assess the capacity of morada nova (mn) and santa inês (sin) rams to maintain body and testicular homeothermy under thermal challenge. for 5 days in the summer, 16 males (sin = 7 and mn = 9) underwent a heat tolerance test, i.e., period 1-animals maintained in the shade (11 to 12 h); period 2-animals exposed to sunlight (12 to 13 h); and period 3-animals returned to the shade (13 to 14 h). the respiratory rate, heart rate, rectal temperature, and infrared surface temperatures ...201728497170
qpcr and hrm-based diagnosis of snps on growth differentiation factor 9 (gdf9), a gene associated with sheep (ovis aries) prolificacy.prolificacy is a desirable trait for genetic improvement of sheep flocks, since it holds the potential to improve productivity. animals carrying single-nucleotide polymorphisms (snps) in genes associated with this trait can be identified and employed to increase prolificacy in flocks. in this study, we report a diagnostic method based on quantitative pcr and high-resolution melting curves to detect different snps in the prolificacy-associated gene growth differentiation factor 9 (gdf9). the diag ...201728667646
domestic sheep show average coxiella burnetii seropositivity generations after a sheep-associated human q fever outbreak and lack detectable shedding by placental, vaginal, and fecal routes.coxiella burnetii is a globally distributed zoonotic bacterial pathogen that causes abortions in ruminant livestock. in humans, an influenza-like illness results with the potential for hospitalization, chronic infection, abortion, and fatal endocarditis. ruminant livestock, particularly small ruminants, are hypothesized to be the primary transmission source to humans. a recent netherlands outbreak from 2007-2010 traced to dairy goats resulted in over 4,100 human cases with estimated costs of mor ...201729141023
new polymorphism in the 5' flanking region of igf-1 gene and its association with wool traits in egyptian barki sheep.insulin-like growth factor-1 gene (igf-1) is considered as a major candidate gene for the economic traits of animal production. polymorphism of 5' flanking region of igf-1 gene in barki sheep (n = 91) and its association with wool traits were studied using the polymerase chain reaction coupled with single-strand conformation polymorphism technique (pcr-sscp), pcr-restriction fragment length polymorphism (pcr-rflp), sequence analysis and different measurements of wool traits (clean fleece weight ...201730647684
isolation and identification of mycoplasma agalactiae by culture and polymerase chain reaction methods in the sheep herds in guilan province, iran.contagious agalactia is an infectious syndrome of sheep that is characterized by mastitis with reduction of milk production, arthritis, abortion, and keratoconjunctivitis. the disease is rapidly spread by the contact of the infected animals with the healthy ones. domestic sheep and goats of both sexes can be infected at an equivalent frequency. most of the researchers use culture and molecular methods for the isolation and identification of mycoplasma. mycoplasma agalactiae is the main cause of ...201730315697
differentiation potential of neural stem cells derived from fetal sheep.neural stem cells (nscs) are multipotent stem cells that can differentiate into many cell types in vitro. in this study, we isolated and established an nsc line from fetal ovis aries. based on the results of immunofluorescence staining, nscs expressed nestin, pax6 and map2. moreover, a reverse transcription-polymerase chain reaction assay was used to biologically characterize the cell line. nscs were induced to differentiate into neurogenic cells in vitro. they expressed map2, glial fibrillary a ...201730460074
the importance of the altricial - precocial spectrum for social complexity in mammals and birds - a review.various types of long-term stable relationships that individuals uphold, including cooperation and competition between group members, define social complexity in vertebrates. numerous life history, physiological and cognitive traits have been shown to affect, or to be affected by, such social relationships. as such, differences in developmental modes, i.e. the 'altricial-precocial' spectrum, may play an important role in understanding the interspecific variation in occurrence of social interacti ...201728115975
Displaying items 301 - 400 of 121503