Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| application of a targeted-enrichment methodology for full-genome sequencing of dengue 1-4, chikungunya and zika viruses directly from patient samples. | the frequency of epidemics caused by dengue viruses 1-4, zika virus and chikungunya viruses have been on an upward trend in recent years driven primarily by uncontrolled urbanization, mobility of human populations and geographical spread of their shared vectors, aedes aegypti and aedes albopictus. infections by these viruses present with similar clinical manifestations making them challenging to diagnose; this is especially difficult in regions of the world hyperendemic for these viruses. in thi ... | 2019 | 31022183 |
| dengue virus reduces expression of low-density lipoprotein receptor-related protein 1 to facilitate replication in aedes aegypti. | aedes aegypti is the primary vector of a number of viruses pathogenic to humans including dengue virus (denv). denv infection leads to widespread transcriptomic and proteomic alterations in mosquito cells. here we identified alterations to the mosquito cell secretome during denv infection by performing liquid chromatography tandem mass spectrometry. we found that an extracellular fragment of low-density lipoprotein receptor-related protein 1 (lrp-1) was present during infection. previous literat ... | 2019 | 31015516 |
| eutrophication governs predator-prey interactions and temperature effects in aedes aegypti populations. | mosquito population dynamics are driven by large-scale (e.g. climatological) and small-scale (e.g. ecological) factors. while these factors are known to independently influence mosquito populations, it remains uncertain how drivers that simultaneously operate under natural conditions interact to influence mosquito populations. we, therefore, developed a well-controlled outdoor experiment to assess the interactive effects of two ecological drivers, predation and nutrient availability, on mosquito ... | 2019 | 31014388 |
| localized control of aedes aegypti (diptera: culicidae) in miami, fl, via inundative releases of wolbachia-infected male mosquitoes. | as part of the response to autochthonous zika transmission in the united states, the city of south miami implemented a 6-mo period in which wolbachia-infected wb1 aedes aegypti (l.) males were released into an ~170-acre area. intracellular wolbachia bacteria infections in ae. aegypti cause early embryonic arrest (known as cytoplasmic incompatibility [ci]) and egg hatch failure, and inundative introductions have been suggested as a potential control tool. throughout the release period, the ae. ae ... | 2019 | 31008514 |
| hierarchical virtual screening of potential insectides inhibitors of acetylcholinesterase and juvenile hormone from temephos. | aedes aegypti (linnaeus, 1762; diptera: culicidae) is the main vector transmitting viral diseases such as dengue fever, dengue haemorrhagic fever, urban yellow fever, zika and chikungunya. worldwide, especially in the americas and brazil, many cases of dengue have been reported in recent years, which have shown significant growth. the main control strategy is the elimination of the vector, carried out through various education programs, to change human habits, but the most usual is biological co ... | 2019 | 31003398 |
| loss of cytoplasmic incompatibility in wolbachia-infected aedes aegypti under field conditions. | wolbachia bacteria are now being introduced into aedes aegypti mosquito populations for dengue control. when wolbachia infections are at a high frequency, they influence the local transmission of dengue by direct virus blocking as well as deleterious effects on vector mosquito populations. however, the effectiveness of this strategy could be influenced by environmental temperatures that decrease wolbachia density, thereby reducing the ability of wolbachia to invade and persist in the population ... | 2019 | 31002720 |
| incrimination of aedes aegypti for dengue virus serotype-1 in assam, northeast india. | 2019 | 30997897 | |
| larvicidal activity of ricinus communis extract against mosquitoes. | vector control strategies play significant role in reducing the transmission of malaria, dengue and other vector-borne diseases. the control of vector population using synthetic insecticides has resulted in development of insecticide resistance and negative effects on humans and environment. the present investigation evaluated the larvicidal potential of methanol, dichloromethane and hexane extracts of leaves and seeds of ricinus communis (castor) plant against the early iv instar larvae of the ... | 2019 | 30997888 |
| tunicates as a biocontrol tool for larvicides acute toxicity of zika virus vector aedes aegypti. | in this present study, we conducted untargeted metabolic profiling using gas chromatography-mass spectrometry (gc-ms) analysis of ascidian didemnum bistratum to assess the chemical constituents by searching in nist library with promising biological properties against anti-bacterial and zika virus vector mosquitocidal properties. metabolites, steroids and fatty acids are abundant in crude compounds of ascidian d. bistratum and showed potential zone growth inhibition against bacterial strains kluy ... | 2019 | 30997309 |
| geographic shifts in aedes aegypti habitat suitability in ecuador using larval surveillance data and ecological niche modeling: implications of climate change for public health vector control. | arboviral disease transmission by aedes mosquitoes poses a major challenge to public health systems in ecuador, where constraints on health services and resource allocation call for spatially informed management decisions. employing a unique dataset of larval occurrence records provided by the ecuadorian ministry of health, we used ecological niche models (enms) to estimate the current geographic distribution of aedes aegypti in ecuador, using mosquito presence as a proxy for risk of disease tra ... | 2019 | 30995228 |
| different susceptibilities of aedes aegypti and aedes albopictus larvae to plant-derived products. | aedes aegypti and aedes albopictus are important vectors that transmit arboviruses to human populations. | 2019 | 30994796 |
| workshop for priority-setting in aedes aegypti control interventions in latin america and the caribbean: a policy dialogue. | this article presents the results of a dialogue between decision-makers and experts in latin america and the caribbean on priority-setting for interventions and studies on aedes aegypti control. the article is part of a project that included a systematic review of mosquito control strategies and a qualitative study with key informants from the region. using a collective deliberative process assisted by the results of the above-mentioned projects, a list of priorities was developed by consensus f ... | 2019 | 30994739 |
| larvicidal activity of ocimum campechianum, ocotea quixos and piper aduncum essential oils against aedes aegypti. | aedes aegypti, the main arbovirus vector of the yellow fever, dengue, chikungunya and zika viruses, is widely distributed in tropical and subtropical areas throughout the world. preventive control efforts have been implemented worldwide aimed at reducing its impact on human health. the recent reduction of chemicals available for vector control due to their negative impact on the environment and human health and the increase in mosquito resistance to insecticides have driven the research communit ... | 2019 | 30994444 |
| downregulation of female doublesex expression by oral-mediated rna interference reduces number and fitness of anopheles gambiae adult females. | mosquito-borne diseases affect millions worldwide, with malaria alone killing over 400 thousand people per year and affecting hundreds of millions. to date, the best strategy to prevent the disease remains insecticide-based mosquito control. however, insecticide resistance as well as economic and social factors reduce the effectiveness of the current methodologies. alternative control technologies are in development, including genetic control such as the sterile insect technique (sit). the sit i ... | 2019 | 30992032 |
| gone in 60 seconds: sub-lethal effects of metofluthrin vapors on behavior and fitness of resistant and field strains of aedes aegypti (diptera: culicidae). | spatial repellents can reduce fecundity and interrupt oviposition behavior in aedes aegypti. yet, it is unclear if short exposure times, resistant phenotypes, and other aspects of spatial repellents can impact these effects on mosquito reproduction. to address these issues, pyrethroid susceptible, pyrethroid resistant, and field strains of ae. aegypti were used to evaluate the extent to which fecundity and oviposition behavior are affected following metofluthrin exposure. mosquitoes were exposed ... | 2019 | 30989189 |
| prevalence of kdr mutations and insecticide susceptibility among natural population of aedes aegypti in west bengal. | aedes albopictus and aedes aegypti are the major vectors of arboviral diseases. as effective vaccines are not available for most of the arboviral diseases, vector control by using insecticides play the key role to reduce the disease transmission. the emergence and spread of resistance to different classes of insecticides by the vectors is a major obstacle to control the disease transmission. information about vector susceptibility to different insecticides and their mechanisms are very important ... | 2019 | 30986273 |
| aedes aegypti hpx8c modulates immune responses against viral infection. | mosquitoes act as vectors of numerous pathogens that cause human diseases. dengue virus (denv) transmitted by mosquito, aedes aegypti, is responsible for dengue fever epidemics worldwide with a serious impact on human health. currently, disease control mainly relies on vector targeted intervention strategies. therefore, it is imperative to understand the molecular mechanisms underlying the innate immune response of mosquitoes against pathogens. in the present study, the expression profiles of im ... | 2019 | 30986216 |
| target and non-target botanical pesticides effect of trichodesma indicum (linn) r. br. and their chemical derivatives against the dengue vector, aedes aegypti l. | the effects of crude ethanol derived leaf extract trichodesma indicum (linn) (ex-ti) and their chief derivatives were accessed on the survival and development of the dengue mosquito ae. aegypti also their non-toxic activity against mosquito predator. t. indicum is recognized to be the vital weed plant and a promising herb in the traditional ayurvedic medicine. in this study, the gc-ms chromatogram of ex-ti showed higher peak area percentage for cis-10-heptadecenoic acid (21.83%) followed by cycl ... | 2019 | 30977009 |
| hsp superfamily of genes in the malaria vector anopheles sinensis: diversity, phylogenetics and association with pyrethroid resistance. | heat shock proteins (hsps) are molecular chaperones that are involved in many normal cellular processes and various kinds of environmental stress. there is still no report regarding the diversity and phylogenetics research of hsp superfamily of genes at whole genome level in insects, and the hsp gene association with pyrethroid resistance is also not well known. the present study investigated the diversity, classification, scaffold location, characteristics, and phylogenetics of the superfamily ... | 2019 | 30975215 |
| neem oil increases the persistence of the entomopathogenic fungus metarhizium anisopliae for the control of aedes aegypti (diptera: culicidae) larvae. | the entomopathogenic fungus metarhizium anisopliae is a candidate for the integrated management of the disease vector mosquito aedes aegypti. metarhizium anisopliae is pathogenic and virulent against ae. aegypti larvae; however, its half-life is short without employing adjuvants. here, we investigated the use of neem oil to increase virulence and persistence of the fungus under laboratory and simulated field conditions. | 2019 | 30975207 |
| a method for assessing mitochondrial physiology using mechanically permeabilized flight muscle of aedes aegypti mosquitoes. | aedes aegypti is the most important and widespread vector of arboviruses, including dengue and zika. insect dispersal through the flight activity is a key parameter that determines vector competence, and is energetically driven by oxidative phosphorylation in flight muscle mitochondria. analysis of mitochondrial function is central for a better understanding of cellular metabolism, and is mostly studied using isolated organelles. however, this approach has several challenges and methods for asse ... | 2019 | 30974092 |
| aedes aegypti nest1 protein enhances zika virus pathogenesis by activating neutrophils. | saliva from the mosquito vector of flaviviruses is capable of changing the local immune environment, leading to an increase in flavivirus-susceptible cells at the infected bite site. in addition, an antibody response to specific salivary gland (sg) components changes the pathogenesis of flaviviruses in human populations. to investigate whether antigenic sg proteins are capable of enhancing infection with zika virus (zikv), a reemerging flavivirus primarily transmitted by the aedes aegypti mosqui ... | 2019 | 30971475 |
| expanding the canon: non-classical mosquito genes at the interface of arboviral infection. | mosquito transmitted viruses cause significant morbidity and mortality in human populations. despite the use of insecticides and other measures of vector control, arboviral diseases are on the rise. one potential solution for limiting disease transmission to humans is to render mosquitoes refractory to viral infection through genetic modification. substantial research effort in drosophila, aedes and anopheles has helped to define the major innate immune pathways, including toll, imd, jak/stat an ... | 2019 | 30970277 |
| abiotic factors and population dynamic of aedes aegypti and aedes albopictus in an endemic area of dengue in brazil. | aedes aegypti and aedes albopictus are exotic species in the americas with high epidemiological relevance as they are vectors of many pathogens. this study aimed at understanding the population dynamics of a. aegypti and a. albopictus and the influence of abiotic factors in an endemic area of dengue. the study was conducted in the urban area of campo grande, mato grosso do sul, brazil, over one year. in seven regions of the city, 50 ovitraps were installed in each neighborhood. the development o ... | 2019 | 30970109 |
| evaluation of the larvicidal potential of the essential oil pogostemon cablin (blanco) benth in the control of aedes aegypti. | the objective of this work was to collect information on the chemical constituents that demonstrate the larvicidal activity against aedes aegypti, as well as the antioxidant, microbiological, and cytotoxicity potential of the essential oil of pogostemon cablin leaves. the chemical characterization was performed by gas chromatography coupled to mass spectrometer (gc-ms). the larvicidal activity was performed according to the protocol of the world health organization. the antioxidant activity was ... | 2019 | 30965561 |
| lack of evidence of sylvatic transmission of dengue viruses in the amazon rainforest near iquitos, peru. | dengue viruses (denv) are currently responsible for more human morbidity and mortality than any other known arbovirus, and all four denv are known to exist in sylvatic cycles that might allow these viruses to persist if the urban (aedes aegypti) cycle could be controlled. to determine whether denv were being maintained in a sylvatic cycle in a forested area about 14 km southwest of iquitos, peru, a city in which all 4 serotypes of denv circulate, we placed 20 denv seronegative aotus monkeys in c ... | 2019 | 30964397 |
| frequent sugar feeding behavior by aedes aegypti in bamako, mali makes them ideal candidates for control with attractive toxic sugar baits (atsb). | current tools and strategies are not sufficient to reliably address threats and outbreaks of arboviruses including zika, dengue, chikungunya, and yellow fever. hence there is a growing public health challenge to identify the best new control tools to use against the vector aedes aegypti. in this study, we investigated ae. aegypti sugar feeding strategies in bamako, mali, to determine if this species can be controlled effectively using attractive toxic sugar baits (atsb). | 2019 | 31206547 |
| the mosquito immune system and the life of dengue virus: what we know and do not know. | flaviviruses are largely transmitted to humans by their arthropod vectors such as mosquitoes or ticks. the dengue virus (denv) is one of the members of the family flaviviridae and is the causative agent of dengue fever. in the mosquito vector, denv enters through viremic blood meal and replicates in the mid-gut. newly formed virion particles circulate to various mosquito organs and get transmitted to the next host in subsequent bites. aedes aegypti and aedes albopictus have intricate immune cont ... | 2019 | 31200426 |
| entomo-virological surveillance strategy for dengue, zika and chikungunya arboviruses in field-caught aedes mosquitoes in an endemic urban area of the northeast of brazil. | aedes spp. are considered the main vectors of dengue (denv), zika (zikv) and chikungunya (chikv) viruses in the world. arbovirus detection in aedes mosquitoes can alert authorities to possible outbreaks, reducing the impact of these diseases. the purpose of this study was to perform an operational strategy for virological surveillance of denv, zikv and chikv in adult aedes aegypti and aedes albopictus mosquitoes captured at different key-sites in an endemic urban area of the northeast region of ... | 2019 | 31194961 |
| surveys of arboviruses vectors in four cities stretching along a railway transect of burkina faso: risk transmission and insecticide susceptibility status of potential vectors. | background: a severe outbreak of dengue occurred in burkina faso in 2016, with the most cases reported in ouagadougou, that highlights the necessity to implement vector surveillance system. this study aims to estimate the risk of arboviruses transmission and the insecticide susceptibility status of potential vectors in four sites in burkina faso. methods: from june to september 2016, house-to-house cross sectional entomological surveys were performed in four cities stretching along a southwest-t ... | 2019 | 31192232 |
| sustained wolbachia-mediated blocking of dengue virus isolates following serial passage in aedes aegypti cell culture. | wolbachia is an intracellular endosymbiont of insects that inhibits the replication of a range of pathogens in its arthropod hosts. the release of wolbachia into wild populations of mosquitoes is an innovative biocontrol effort to suppress the transmission of arthropod-borne viruses (arboviruses) to humans, most notably dengue virus. the success of the wolbachia-based approach hinges upon the stable persistence of the 'pathogen blocking' effect, whose mechanistic basis is poorly understood. evid ... | 2019 | 31191980 |
| phylogenetic analysis of dengue-2 serotypes circulating in mangroves in northern cordoba, colombia. | in this study, we aimed to identify denv-2 subtypes in aedes aegypti pools collected between 2011 and 2017 in a rural area of northern cordoba, colombia ("la balsa"). | 2019 | 31188919 |
| vector competence of aedes aegypti for different strains of zika virus in argentina. | the importance of zika virus (zikv) has increased noticeably since the outbreak in the americas in 2015, when the illness was associated with congenital disorders. although there is evidence of sexual transmission of the virus, the mosquito aedes aegypti is believed to be the main vector for transmission to humans. this species of mosquito has not only been found naturally infected with zikv, but also has been the subject of study in many vector competence assays that employ different strains of ... | 2019 | 31188869 |
| optimal control of vaccination in a vector-borne reaction-diffusion model applied to zika virus. | zika virus has acquired worldwide concern after a recent outbreak in latin america that started in brazil, with associated neurological conditions such as microcephaly in newborns from infected mothers. the virus is transmitted mainly by aedes aegypti mosquitoes, but direct (sexual) transmission has been documented. we formulate a reaction diffusion model that considers spatial movement of humans and vectors, with local contact transmission of zika virus. vaccination is introduced as a control v ... | 2019 | 31187254 |
| cartography of odor chemicals in the dengue vector mosquito (aedes aegypti l., diptera/culicidae). | this study was aimed to identify the chemical compounds of aedes aegypti that can be potentially used to develop pheromone-based vector control methods. in this study, we compared the chemical compounds collected from the organs of mosquitoes at different developmental stages in the life cycle. we also compared the composition and amount of extracts from the different tissues of male and female adult mosquito. interestingly, we found large amount of c17-c20 ethyl and methyl esters in the wings o ... | 2019 | 31186462 |
| male competition and the evolution of mating and life-history traits in experimental populations of aedes aegypti. | aedes aegypti is an important disease vector and a major target of reproductive control efforts. we manipulated the opportunity for sexual selection in populations of ae. aegypti by controlling the number of males competing for a single female. populations exposed to higher levels of male competition rapidly evolved higher male competitive mating success relative to populations evolved in the absence of competition, with an evolutionary response visible after only five generations. we also detec ... | 2019 | 31185872 |
| assessing the potential interactions between cellular mirna and arboviral genomic rna in the yellow fever mosquito, aedes aegypti. | although the role of exogenous small interfering rna (sirna) and p-element induced wimpy testis (piwi)-interacting rna (pirna) pathways in mosquito antiviral immunity is increasingly better understood, there is still little knowledge regarding the role of mosquito cellular microrna (mirna). identifying direct interactions between the mosquito mirnas and the rna genome of arboviruses and choosing the relevant mirna candidates to explore resulting antiviral mechanisms are critical. here, we carrie ... | 2019 | 31185697 |
| seasonal and geographic variation in insecticide resistance in aedes aegypti in southern ecuador. | insecticide resistance (ir) can undermine efforts to control vectors of public health importance. aedes aegypti is the main vector of resurging diseases in the americas such as yellow fever and dengue, and recently emerging chikungunya and zika fever, which have caused unprecedented epidemics in the region. vector control remains the primary intervention to prevent outbreaks of aedes-transmitted diseases. in many high-risk regions, like southern ecuador, we have limited information on ir. in thi ... | 2019 | 31181073 |
| screening aedes aegypti (diptera: culicidae) populations from pernambuco, brazil for resistance to temephos, diflubenzuron, and cypermethrin and characterization of potential resistance mechanisms. | resistance to chemical insecticides detected in aedes aegypti (l.) mosquitoes has been a problem for the national dengue control program (pncd) over the last years. in order to provide deeper knowledge of resistance to xenobiotics, our study evaluated the susceptibility profile of temephos, diflubenzuron, and cypermethrin insecticides in natural mosquito populations from the pernambuco state, associating these results with the local historical use of such compounds. furthermore, mechanisms that ... | 2019 | 31175834 |
| temperature impacts on dengue emergence in the united states: investigating the role of seasonality and climate change. | tropical mosquito-borne viruses have been expanding into more temperate regions in recent decades. this is partly due to the coupled effects of temperature on mosquito life history traits and viral infection dynamics and warming surface temperatures, resulting in more suitable conditions for vectors and virus transmission. in this study, we use a deterministic ordinary differential equations model to investigate how seasonal and diurnal temperature fluctuations affect the potential for dengue tr ... | 2019 | 31175008 |
| enhanced vector surveillance to control arbovirus epidemics in colombia. | in the wake of the zika epidemic, there has been intensified interest in the surveillance and control of the arbovirus vectors aedes aegypti and aedes albopictus, yet many existing surveillance systems could benefit from improvements. vector control programs are often directed by national governments, but are carried out at the local level, resulting in the discounting of spatial heterogeneities in ecology and epidemiology. furthermore, entomological and epidemiological data are often collected ... | 2019 | 31171922 |
| community assessments for mosquito prevention and control experiences, attitudes, and practices - u.s. virgin islands, 2017 and 2018. | aedes aegypti, the mosquito that carries dengue, chikungunya, and zika viruses, is present throughout the u.s. virgin islands (usvi). to reduce mosquitoborne disease transmission, the usvi department of health (vidoh) is responsible for integrated mosquito management. during january 2016-january 2018, usvi experienced its first zika outbreak, with most cases reported during january-december 2016, as well as two category 5 hurricanes (irma on st. thomas/st. john on september 6, 2017, and maria on ... | 2019 | 31170124 |
| the widespread occurrence and potential biological roles of endogenous viral elements in insect genomes. | modern genomic sequencing and bioinformatics approaches have detected numerous examples of dna sequences derived from dna and rna virus genomes integrated into both vertebrate and insect genomes. retroviruses encode rna-dependent dna polymerases (reverse transcriptases) and integrases that convert their rna viral genomes into dna proviruses and facilitate proviral dna integration into the host genome. surprisingly, dna sequences derived from rna viruses that do not encode these enzymes also occu ... | 2019 | 31167954 |
| rnaseq analysis of aedes albopictus mosquito midguts after chikungunya virus infection. | chikungunya virus (chikv) is an emerging pathogen around the world and causes significant morbidity in patients. a single amino acid mutation in the envelope protein of chikv has led to a shift in vector preference towards aedesalbopictus. while mosquitoes are known to mount an antiviral immune response post-infection, molecular interactions during the course of infection at the tissue level remain largely uncharacterised. we performed whole transcriptome analysis on dissected midguts of aedes a ... | 2019 | 31167461 |
| aedes aegypti microrna, mir-2944b-5p interacts with 3'utr of chikungunya virus and cellular target vps-13 to regulate viral replication. | rna interference is among the most important mechanisms that serve to restrict virus replication within mosquitoes, where micrornas (mirnas) are important in regulating viral replication and cellular functions. these mirnas function by binding to complementary sequences mostly in the untranslated regions of the target. chikungunya virus (chikv) genome consists of two open reading frames flanked by 5' and 3' untranslated regions on the two sides. a recent study from our laboratory has shown that ... | 2019 | 31166953 |
| utilizing larvicidal and pupicidal efficacy of eucalyptus and neem oil against aedes mosquito: an approach for mosquito control. | plant-based products can provide safe and biodegradable mosquito control agents. the essential oils have a strong odor due to complex secondary metabolites and exhibit lower density than that of water, which renders them suitable to form a thin layer above the water surface. the present study was designed to evaluate the larvicidal, pupicidal activity of eucalyptus and neem oils against aedes aegypti and aedes albopictus. | 2019 | 31161087 |
| wolbachia pipientis occurs in aedes aegypti populations in new mexico and florida, usa. | the mosquitoes aedes aegypti (l.) and ae. albopictus skuse are the major vectors of dengue, zika, yellow fever, and chikungunya viruses worldwide. wolbachia, an endosymbiotic bacterium present in many insects, is being utilized in novel vector control strategies to manipulate mosquito life history and vector competence to curb virus transmission. earlier studies have found that wolbachia is commonly detected in ae. albopictus but rarely detected in ae. aegypti. in this study, we used a two-step ... | 2019 | 31161026 |
| molecular evidence of sequential evolution of ddt- and pyrethroid-resistant sodium channel in aedes aegypti. | multiple mutations in the voltage-gated sodium channel have been associated with knockdown resistance (kdr) to ddt and pyrethroid insecticides in a major human disease vector aedes aegypti. one mutation, v1016g, confers sodium channel resistance to pyrethroids, but a different substitution in the same position v1016i alone had no effect. in pyrethroid-resistant ae. aegypti populations, v1016i is often linked to another mutation, f1534c, which confers sodium channel resistance only to type i pyre ... | 2019 | 31158225 |
| blood feeding activates the vitellogenic stage of oogenesis in the mosquito aedes aegypti through inhibition of glycogen synthase kinase 3 by the insulin and tor pathways. | most mosquitoes, including aedes aegypti, only produce eggs after blood feeding on a vertebrate host. oogenesis in a. aegypti consists of a pre-vitellogenic stage before blood feeding and a vitellogenic stage after blood feeding. primary egg chambers remain developmentally arrested during the pre-vitellogenic stage but complete oogenesis to form mature eggs during the vitellogenic stage. in contrast, the signaling factors that maintain primary egg chambers in pre-vitellogenic arrest or that acti ... | 2019 | 31153832 |
| vapor delivery of plant essential oils alters pyrethroid efficacy and detoxification enzyme activity in mosquitoes. | the use of synthetic insecticides to limit the spread of mosquito-borne disease faces a number of significant challenges, including insecticide resistance, concerns related to the environmental impact of widespread insecticide use, as well as slowed development of new insecticide chemistries. one important alternative to broadcast insecticides is the use of personal protection strategies to limit contact with vector species, including the use of spatial repellents that can employ synthetic pyret ... | 2019 | 31153481 |
| transmission of lumpy skin disease virus: a short review. | lumpy skin disease (lsd) is a viral transboundary disease endemic throughout africa and of high economic importance that affects cattle and domestic water buffaloes. since 2012, the disease has spread rapidly and widely throughout the middle eastern and balkan regions, southern caucasus and parts of the russian federation. before vaccination campaigns took their full effect, the disease continued spreading from region to region, mainly showing seasonal patterns despite implementing control and e ... | 2019 | 31152757 |
| barriers that limit the implementation of thermal fogging for the control of dengue in colombia: a study of mixed methods. | thermal fogging of insecticides is a vector control strategy used by the medellin secretary of health to combat dengue. this method is employed during outbreaks to curb populations of potentially infectious adult mosquitoes and interrupt transmission cycles. while this strategy has been used in medellin since 2007, in some years it has not reduced dengue cases as expected. difficulties in the implementation of fumigation strategies, such as lack of opportunity for treatment and public perception ... | 2019 | 31146722 |
| estimating the impact of city-wide aedes aegypti population control: an observational study in iquitos, peru. | during the last 50 years, the geographic range of the mosquito aedes aegypti has increased dramatically, in parallel with a sharp increase in the disease burden from the viruses it transmits, including zika, chikungunya, and dengue. there is a growing consensus that vector control is essential to prevent aedes-borne diseases, even as effective vaccines become available. what remains unclear is how effective vector control is across broad operational scales because the data and the analytical too ... | 2019 | 31145744 |
| effects of constant and fluctuating low temperatures on the development of aedes aegypti (diptera: culicidae) from a temperate region. | most studies of the effects of low temperature on the development of immature stages of aedes aegypti (l.) have been performed at constant temperatures in the laboratory, which may not accurately reflect the variable environmental conditions in the field. thus, the aim of this study was to assess the effect of constant temperatures (ct) and fluctuating low temperatures (ft) on the fitness of ae. aegypti of buenos aires, argentina. three ct treatments (12, 14, and 16°c) and three ft treatments (1 ... | 2019 | 31139823 |
| predatory efficacy of five locally available copepods on aedes larvae under laboratory settings: an approach towards bio-control of dengue in sri lanka. | many countries are in search of more effective and sustainable methods for controlling dengue vectors, due to undeniable inefficiencies in chemical and mechanical vector control methods. bio-control of vectors by copepods is an ideal method of using interactions in the natural ecosystem for vector management, with minimum consequences on the environment. current study determined the predatory efficacy of five locally abundant copepod species on, aedes larvae under laboratory conditions. copepods ... | 2019 | 31136574 |
| chemical composition and larvicidal activity of the essential oil from the leaves of onychopetalum periquino (rusby) d.m. johnson & n.a. murray. | the essential oil (eo) from the leaves of onychopetalum periquino, obtained by hydrodistillation, was analyzed by gas chromatography coupled with mass spectrometry (gc-ms), and also was investigated for its larvicidal activity against aedes aegypti larvae. thirteen compounds, representing 91.31% of the crude oil, were identified. major compounds were sesquiterpenes, including β-elemene (53.16%), spathulenol (11.94%) and β-selinene (9.25%). the eo showed high larvicidal activity with a lethal con ... | 2019 | 31135221 |
| sodium ascorbate as a potential toxicant in attractive sugar baits for control of adult mosquitoes (diptera: culicidae) and sand flies (diptera: psychodidae). | attractive toxic sugar baits (atsbs) can be an effective vector control tool, especially in areas where aerial or aquatic applications of pesticides are undesirable or impractical. in general, there is a need to develop novel or alternative insecticides for vector control, and there is a demand from consumers for more 'natural' pest control products. sodium ascorbate (sa) is a naturally occurring antioxidant compound, found in fruits and vegetables, and is available commercially in the united st ... | 2019 | 31134270 |
| high infestation of invasive aedes mosquitoes in used tires along the local transport network of panama. | the long-distance dispersal of the invasive disease vectors aedes aegypti and aedes albopictus has introduced arthropod-borne viruses into new geographical regions, causing a significant medical and economic burden. the used-tire industry is an effective means of aedes dispersal, yet studies to determine aedes occurrence and the factors influencing their distribution along local transport networks are lacking. to assess infestation along the primary transport network of panama we documented all ... | 2019 | 31133041 |
| the sulcatone receptor of the strict nectar-feeding mosquito toxorhynchites amboinensis. | controlling ae. aegypti populations and the prevention of mosquito bites includes the development of monitoring, repelling and attract-and-kill strategies that are based on understanding the chemical ecology of these pests. olfactory-mediated attraction to mammals has recently been linked to the mosquito aedes aegypti odorant receptor or4, which is activated by animal-released 6-methyl-5-hepten-2-one (sulcatone). this odorant is also a major component of flower scents and may play a role outside ... | 2019 | 31129164 |
| a genomic approach to inferring kinship reveals limited intergenerational dispersal in the yellow fever mosquito. | understanding past dispersal and breeding events can provide insight into ecology and evolution and can help inform strategies for conservation and the control of pest species. however, parent-offspring dispersal can be difficult to investigate in rare species and in small pest species such as mosquitoes. here, we develop a methodology for estimating parent-offspring dispersal from the spatial distribution of close kin, using pairwise kinship estimates derived from genome-wide single nucleotide ... | 2019 | 31125998 |
| fractional-dose yellow fever vaccination: how much more can we do with less? | climate change, deforestation, urbanization, and increased population mobility have made the risk of large outbreaks of yellow fever more likely than ever. yellow fever vaccine production barely meets demands. in this review, we address the causes of the recent yellow fever outbreaks, why fractional dose yellow fever vaccination works, the role of virus neutralizing antibodies in the protection against yellow fever, and the need for revaccination. | 2019 | 31335440 |
| using citizen science to enhance surveillance of aedes aegypti in arizona, 2015-17. | vector surveillance is an essential component of vector-borne disease prevention, but many communities lack resources to support extensive surveillance. the great arizona mosquito hunt (gamh) was a collaborative citizen science project conducted during 2015-17 to enhance surveillance for aedes aegypti in arizona. citizen science projects engage the public in scientific research in order to further scientific knowledge while improving community understanding of a specific field of science and the ... | 2019 | 31334498 |
| a novel densovirus isolated from the asian tiger mosquito displays varied pathogenicity depending on its host species. | mosquito-borne viral diseases (mbvds) continue to pose a significant global public health burden. mosquito control remains a core intervention strategy in integrated mosquito management (imm) programs to reduce the transmission of mbvds. mosquito densoviruses (mdvs) are mosquito-specific entomopathogenic viruses, and their attractive biological and pathogenic characteristics make mdvs potential biological control agents as alternatives to traditional chemical pesticides. however, different viral ... | 2019 | 31333635 |
| 'swimming mosquitoes': a key stepping stone to prevent dengue, zika and chikungunya: an educative experience in colima, mexico. | dengue, zika and chikungunya arboviruses (dzc), mainly transmitted by mosquito aedes aegypti, are global health public issues affecting 390 million people each year. in most endemic countries, vector control strategies have been focused on reducing breeding sites and adult mosquito populations. most health communication strategies have pointed out adult mosquito as the main responsible of the transmission, persuading people to eliminate domestic containers. however, it is not clear how should pe ... | 2019 | 31329869 |
| susceptibility and alterations by diflubenzuron in larvae of aedes aegypti. | benzoylphenyl ureas inhibit chitin synthesis and interfere with the molting process in arthropods. in this study, the effect of diflubenzuron on third-instar larvae of aedes aegypti was evaluated. the susceptibility to the product was determined, and the alterations generated were shown through light and electron microscopy. lc50 and lc90 were 0.23 and 0.47 ppm, respectively. the main alterations observed were the incapacity to complete the molt, a reduction of mobility, the fragmentation of the ... | 2019 | 31328823 |
| visual-olfactory integration in the human disease vector mosquito aedes aegypti. | mosquitoes rely on the integration of multiple sensory cues, including olfactory, visual, and thermal stimuli, to detect, identify, and locate their hosts [1-4]. although we increasingly know more about the role of chemosensory behaviors in mediating mosquito-host interactions [1], the role of visual cues is comparatively less studied [3], and how the combination of olfactory and visual information is integrated in the mosquito brain remains unknown. in the present study, we used a tethered-flig ... | 2019 | 31327719 |
| mosquito host-seeking regulation: targets for behavioral control. | female aedes aegypti mosquitoes require protein from blood to develop eggs. they have evolved a strong innate drive to find and bite humans and engorge on their blood. decades of research have revealed that attraction to hosts is suppressed for days after blood-feeding. during this time, females coordinate complex physiological changes, allowing them to utilize blood protein to develop eggs: clearing excess fluid, digesting protein, and egg maturation. how do mechanosensation, nutrient consumpti ... | 2019 | 31326312 |
| too "sexy" for the field? paired measures of laboratory and semi-field performance highlight variability in the apparent mating fitness of aedes aegypti transgenic strains. | evaluating and improving mating success and competitive ability of laboratory-reared transgenic mosquito strains will enhance the effectiveness of proposed disease-control strategies that involve deployment of transgenic strains. two components of the mosquito rearing process, larval diet quantity and aquatic environment - which are linked to physiological and behavioural differences in adults - are both relatively easy to manipulate. in mosquitoes, as for many other arthropod species, the quali ... | 2019 | 31324262 |
| hydrophobic nanoprecipitates formed by benzoylphenylureas and β-cyclodextrin inclusion compounds: synthesis, characterization and toxicity against aedes aegypti larvae. | the aim of this work was to synthesize and characterize the inclusion compounds formed by the complexation of β-cyclodextrin (βcd) with insecticides from the class of benzoylphenylureas (bpus), named novaluron (nov) and diflubenzuron (dif), beyond evaluate their larvicidal activity against aedes aegypti larvae. solid state characterization by ftir showed changes in the main peaks of bpus and βcd, suggesting the formation of inclusion compounds in solid phase. dta and tga thermal analysis showed ... | 2019 | 31321326 |
| mosquito vectors of arboviruses in french polynesia. | the circulation of arthropod-borne viruses (arboviruses) throughout the pacific is no exception in french polynesia (fp). we review here the mosquito vectors involved or potentially involved in the transmission of arboviruses in fp. we highlight aedes aegypti and aedes polynesiensis; species that are widely distributed in fp and whose ability to transmit dengue, zika and chikungunya viruses is well known. in addition, we discuss the contribution of those species and the abundant culex annulirost ... | 2019 | 31316821 |
| evaluation of inflammatory skin infiltrate following aedes aegypti bites in sensitized and non-sensitized mice reveals saliva-dependent and immune-dependent phenotypes. | during probing and blood feeding, haematophagous mosquitoes inoculate a mixture of salivary molecules into their vertebrate hosts' skin. in addition to the anti-haemostatic and immunomodulatory activities, mosquito saliva also triggers acute inflammatory reactions, especially in sensitized hosts. here, we characterize the oedema and the cellular infiltrate following aedes aegypti mosquito bites in the skin of sensitized and non-sensitized balb/c mice by flow cytometry. ae. aegypti bites induced ... | 2019 | 31315156 |
| agbr1 antibodies delay lethal aedes aegypti-borne west nile virus infection in mice. | west nile virus (wnv) is transmitted by mosquitoes and can cause severe disease, including meningoencephalitis. agbr1 is a mosquito salivary protein that enhances aedes aegypti mosquito-borne zika virus pathogenesis in mice. here, we show that agbr1 antibodies reduce the initial west nile viral load and delay lethal infection after feeding by an infected aedes aegypti mosquito. targeting agbr1 may therefore be incorporated into strategies to prevent mosquito-transmitted west nile virus infection ... | 2019 | 31312526 |
| natural vertical transmission of zika virus in larval aedes aegypti populations, morelos, mexico. | we characterized natural vertical transmission of zika virus in pools of aedes aegypti larvae hatched from eggs collected in jojutla, morelos, mexico. of the 151 pools analyzed, 17 tested positive for zika virus rna; infectious zika virus was successfully isolated from 1 of the larvae pools (31n) in c6/36 cells. real-time quantitative pcr and indirect immunofluorescence assays confirmed the identity of the isolate, named zika virus isolate 31n; plaque assays in vero cells demonstrated the isolat ... | 2019 | 31310224 |
| expression of bacillus thuringiensis toxin cyt2ba in the entomopathogenic fungus beauveria bassiana increases its virulence towards aedes mosquitoes. | the entomopathogenic fungus beauveria bassiana has been widely used to kill mosquito larvae and adults in the laboratory and field. however, its slow action of killing has hampered its widespread application. in our study, the b. bassiana fungus was genetically modified to express the bacillus thuringiensis (bt) toxin cyt2ba to improve its efficacy in killing mosquitoes. | 2019 | 31306427 |
| ai- modelling of molecular identification and feminization of wolbachia infected aedes aegypti. | the genetic control strategies of vector borne diseases includes the replacement of a vector population by "disease-refractory" mosquitoes and the release of mosquitoes with a gene to control the vector's reproduction rates. wolbachia are common intracellular bacteria that are found in arthropods and nematodes. wolbachia infected male mosquitos have been used in different experimental trials around the world to suppress the target population of aedes aegypti and this genetic control strategy has ... | 2019 | 31302148 |
| eucalyptus camaldulensis properties for use in the eradication of infections. | eucalyptus camaldulensis (e. camaldulensis), called the eucalyptus has so many characteristics such as antimicrobial features. common names include red gum, red chewing gum, river chewing gum, red chewing gum. its class is eucalyptus, which is comprised of 800 species worldwide, but three or four species are found in australia. this tree generally grows on the edge of rivers with continuous or seasonal water. most of the gray clay soils run along the riverside and are exposed to frequent floods, ... | 2019 | 31300120 |
| tracking genetic invasions: genome-wide single nucleotide polymorphisms reveal the source of pyrethroid-resistant aedes aegypti (yellow fever mosquito) incursions at international ports. | biological invasions are increasing globally in number and extent despite efforts to restrict their spread. knowledge of incursion pathways is necessary to prevent new invasions and to design effective biosecurity protocols at source and recipient locations. this study uses genome-wide single nucleotide polymorphisms (snps) to determine the origin of 115 incursive aedes aegypti(yellow fever mosquito) detected at international ports in australia and new zealand. we also genotyped mosquitoes at th ... | 2019 | 31297145 |
| location, seasonal and functional characteristics of water-holding containers with juvenile aedes albopictus in urban southern taiwan: a cross-sectional study. | urban kaohsiung city (kc), in southern taiwan, has experienced annual dengue epidemics for decades despite considerable vector control efforts. substantial research has been undertaken to characterize aedes aegypti breeding habitats, but no systematic assessment has been completed for aedes albopictus present in kc. | 2019 | 31294804 |
| development of the major arboviral vector aedes aegypti in urban drain-water and associated pyrethroid insecticide resistance is a potential global health challenge. | aedes aegypti were found developing in the water in open public drains (drain-water, dw) in jaffna city in northern sri lanka, a location where the arboviral diseases dengue and chikungunya are endemic. | 2019 | 31287014 |
| a mosquito workshop and community intervention: a pilot education campaign to identify risk factors associated with container mosquitoes in san pedro sula, honduras. | dengue poses a significant public health threat and results in ~96 million clinical cases every year. central america is a region burdened by neglected tropical diseases, including dengue. the primary vectors of dengue, aedes aegypti and aedes albopictus, are widely distributed in honduras. additionally, sustained and consistent mosquito control is lacking in the country. successful control of container mosquitoes relies heavily on participation from community leaders, stakeholders, and the comm ... | 2019 | 31284544 |
| intercepted mosquitoes at new zealand's ports of entry, 2001 to 2018: current status and future concerns. | mosquito vectors are extending their range via international travel and trade. climate change makes new zealand an increasingly suitable environment for less tropically adapted exotic mosquito vectors to become established. this shift will add a multiplier effect to existing risks of both the establishment of new species and of resident exotic species extending into new areas. we describe trends in the border interceptions of exotic mosquitoes and evaluate the role of imported goods as a pathway ... | 2019 | 31284464 |
| vertical transmission of zika virus (flaviviridae, flavivirus) in amazonian aedes aegypti (diptera: culicidae) delays egg hatching and larval development of progeny. | zika virus (zikv) has emerged as a globally important arbovirus and has been reported from all states of brazil. the virus is primarily transmitted to humans through the bite of an infective aedes aegypti (linnaeus, 1762) or aedes albopictus (skuse, 1895). however, it is important to know if zikv transmission also occurs from ae. aegypti through infected eggs to her offspring. therefore, a zikv and dengue virus (denv) free colony was established from eggs collected in manaus and maintained until ... | 2019 | 31278737 |
| using genetic variation in aedes aegypti to identify candidate anti-dengue virus genes. | transcriptomic profiling has generated extensive lists of genes that respond to viral infection in mosquitoes. these gene lists contain two types of genes; (1) those that are responsible for the insect's natural antiviral defense mechanisms, including some known innate immunity genes, and (2) genes whose change in expression may occur simply as a result of infection. as genetic modification tools for mosquitoes continue to improve, the opportunities to make refractory insects via allelic replace ... | 2019 | 31272403 |
| spatial distribution of aedes aegypti (diptera: culicidae) in vulnerable areas for the transmission of arboviruses. | areas at risk of transmission of arboviruses have been monitored using ovitraps. this study aimed to evaluate the spatial distribution of aedes aegypti in vulnerable areas for the transmission of arboviruses and assess the influence of climatic conditions on the infestation of these culicids. | 2019 | 31271613 |
| insecticide resistance status of aedes aegypti and aedes albopictus mosquitoes in papua new guinea. | aedes aegypti and ae. albopictus are important vectors of infectious diseases, especially those caused by arboviruses such as dengue, chikungunya and zika. aedes aegypti is very well adapted to urban environments, whereas ae. albopictus inhabits more rural settings. pyrethroid resistance is widespread in these vectors, but limited data exist from the southwest pacific region, especially from melanesia. while aedes vector ecology is well documented in australia, where incursion of ae. albopictus ... | 2019 | 31269965 |
| aedes vector surveillance in the southeastern united states reveals growing threat of aedes japonicus japonicus (diptera: culicidae) and aedes albopictus. | mosquito-borne diseases are on the rise globally, and have the potential to thrive along the gulf coast of the united states, where subtropical conditions may facilitate the introduction or movement of mosquito vectors. despite surveillance efforts, aedes aegypti (l.) had not been detected in the gulf state of alabama for nearly three decades. the detection of ae. aegypti in alabama may suggest remnant or reemergent populations of this vector. we conducted adult sampling between may and august o ... | 2019 | 31268136 |
| a salivary protein of aedes aegypti promotes dengue-2 virus replication and transmission. | although dengue is the most prevalent arthropod-borne viral disease in humans, no effective medication or vaccine is presently available. previous studies suggested that mosquito salivary proteins influence infection by the dengue virus (denv) in the mammalian host. however, the effects of salivary proteins on denv replication within the aedes aegypti mosquito remain largely unknown. in this study, we investigated the effect of a specific salivary protein (named aasg34) on denv serotype 2 (denv2 ... | 2019 | 31265906 |
| inhibition of juvenile hormone synthesis in mosquitoes by the methylation inhibitor 3-deazaneplanocin a (dznep). | juvenile hormone (jh), synthesized by the corpora allata (ca), controls development and reproduction in mosquitoes through its action on thousands of jh-responsive genes. these jh-dependent processes can be studied using tools that increase or decrease jh titers in vitro and in vivo. juvenile hormone acid methyl transferase (jhamt) is a critical jh biosynthetic enzyme. jhamt utilizes the methyl donor s-adenosyl-methionine (sam) to methylate farnesoic acid (fa) into methyl farnesoate (mf), releas ... | 2019 | 31265905 |
| combinations of plant essential oil based terpene compounds as larvicidal and adulticidal agent against aedes aegypti (diptera: culicidae). | insecticidal plant-based compound(s)in combinations may show synergistic or antagonistic interactions against insect pest. considering the rapid spread of the aedes borne diseases and increasing resistance among aedes population against conventional insecticides, twenty-eight combinations of plant essential oil-based terpene compounds were prepared and tested against larval and adult stages ofaedes aegypti. initially five plant essential oils (eos) were assessed for their larvicidal and adultici ... | 2019 | 31263222 |
| pluripotency of wolbachia against arboviruses: the case of yellow fever. | background: yellow fever outbreaks have re-emerged in brazil during 2016-18, with mortality rates up to 30%. although urban transmission has not been reported since 1942, the risk of re-urbanization of yellow fever is significant, as aedes aegypti is present in most tropical and sub-tropical cities in the world and still remains the main vector of urban yfv. although the yfv vaccine is safe and effective, it does not always reach populations at greatest risk of infection and there is an acknowle ... | 2019 | 31259313 |
| general visual and contingent thermal cues interact to elicit attraction in female aedes aegypti mosquitoes. | female aedes aegypti mosquitoes use multiple sensory modalities to hunt human hosts and obtain a blood meal for egg production. attractive cues include carbon dioxide (co2), a major component of exhaled breath [1, 2]; heat elevated above ambient temperature, signifying warm-blooded skin [3, 4]; and dark visual contrast [5, 6], proposed to bridge long-range olfactory and short-range thermal cues [7]. any of these sensory cues in isolation is an incomplete signal of a human host, and so a mosquito ... | 2019 | 31257144 |
| transcriptomic analysis of aedes aegypti innate immune system in response to ingestion of chikungunya virus. | aedes aegypti (l.) is the primary vector of emergent mosquito-borne viruses, including chikungunya, dengue, yellow fever, and zika viruses. to understand how these viruses interact with their mosquito vectors, an analysis of the innate immune system response was conducted. the innate immune system is a conserved evolutionary defense strategy and is the dominant immune system response found in invertebrates and vertebrates, as well as plants. rna-sequencing analysis was performed to compare targe ... | 2019 | 31252518 |
| estimating past, present, and future trends in the global distribution and abundance of the arbovirus vector aedes aegypti under climate change scenarios. | background:aedes aegypti is the principal vector for several important arbovirus diseases, including dengue, chikungunya, yellow fever, and zika. while recent empirical research has attempted to identify the current global distribution of the vector, the seasonal, and longer-term dynamics of the mosquito in response to trends in climate, population, and economic development over the twentieth and the twenty-first century remains to be elucidated. methods: in this study, we use a process-based ma ... | 2019 | 31249824 |
| wolbachia prevalence, diversity, and ability to induce cytoplasmic incompatibility in mosquitoes. | to protect humans and domestic animals from mosquito borne diseases, alternative methods to chemical insecticides have to be found. pilot studies using the vertically transmitted bacterial endosymbiont wolbachia were already launched in different parts of the world. wolbachia can be used either in incompatible insect technique (iit), to decrease mosquito population, or to decrease the ability of mosquitoes to transmit pathogens. not all mosquito species are naturally infected with wolbachia: whi ... | 2019 | 31247412 |
| the taste of water. | female mosquitos require a specific ion-channel protein to sense the presence of fresh water in which they can lay their eggs. | 2019 | 31246169 |
| the basic plan of the adult heart is conserved across different species of adult mosquitoes, but the morphology of heart-associated tissues varies. | the heart is a pivotal organ in insects because it performs a number of different tasks, such as circulating nutrients, hormones, and excreta. in this study, the morphologies of the heart and associated tissues, including pericardial cells (pcs) and alary muscles (ams), in the hematophagous mosquitoes anopheles aquasalis curry (diptera: culicidae), aedes aegypti l. (diptera: culicidae), and culex quinquefasciatus say (diptera: culicidae), and the phytophagous toxorhynchites theobaldi dyar & knab ... | 2019 | 31245826 |
| pcr-based bloodmeal analysis of aedes aegypti and culex quinquefasciatus (diptera: culicidae) in st. george parish, grenada. | blood-feeding patterns of mosquitoes affect the transmission and maintenance of arboviral diseases. in the caribbean, aedes aegypti (l.) and culex quinquefasciatus say mosquitoes are the dominant mosquito species in developed areas. however, no information is available on the bloodmeal hosts of these invasive vectors in grenada, where arboviral pathogens such as dengue, chikungunya, and zika viruses cause significant human suffering. to this end, ae. aegypti and cx. quinquefasciatus mosquitoes w ... | 2019 | 31245825 |
| mosquito larvicidal potential of hydroxy-2-methyl-4h-pyran-4-one (maltol) isolated from the methanol root extract of senecio laetus edgew. and its in-silico study. | in the present study, hydroxy-2-methyl-4h-pyran-4-one was isolated from the methanol root extract of senecio laetus and was identified by ft-ir, 1h, 13c nmr and gc-ms. the larvicidal potential of the compound 3-hydroxy-2-methyl-4h-pyran-4-one was evaluated against the 4th instar larvae of anopheles stephensi, aedes aegypti and culex quinquefasciatus at concentrations ranging from 20 to 1.25 ppm under laboratory conditions. the compound showed 100% mortality at 20 ppm against all the tested mosqu ... | 2019 | 31244340 |
| a t164s mutation in the dengue virus ns1 protein is associated with greater disease severity in mice. | dengue viruses cause severe and sudden human epidemics worldwide. the secreted form of the nonstructural protein 1 (sns1) of dengue virus causes vascular leakage, a hallmark of severe dengue disease. here, we reverse engineered the t164s mutation of ns1, associated with the severity of dengue epidemics in the americas, into a dengue virus serotype 2 mildly infectious strain. the t164s mutant virus decreased infectious virus production and increased sns1 production in mammalian cell lines and hum ... | 2019 | 31243154 |
| cell-fusing agent virus reduces arbovirus dissemination in aedes aegypti mosquitoes in vivo. | aedes aegypti mosquitoes are the main vectors of arthropod-borne viruses (arboviruses) of public health significance, such as the flaviviruses dengue virus (denv) and zika virus (zikv). mosquitoes are also the natural hosts of a wide range of viruses that are insect specific, raising the question of their influence on arbovirus transmission in nature. cell-fusing agent virus (cfav) was the first described insect-specific flavivirus, initially discovered in an a. aegypti cell line and subsequentl ... | 2019 | 31243123 |