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vasoactive intestinal peptide-stimulated glycoconjugate secretion from conjunctival goblet cells.we have developed an in vitro and in vivo method to determine if vip-stimulates conjunctival goblet cell secretion in the rat as nerves which contain vasoactive intestinal peptide (vip) and are most likely parasympathetic are localized around these cells. for the in vitro method, pieces of rat conjunctiva were incubated for 1 hr with no additions or increasing concentrations of vip (10(-10)-10(-6)m). goblet cell secretion was measured by determining the amount of helix pomatia agglutinin (hpa)-d ...19968983961
synapsin-like molecules in aplysia punctata and helix pomatia: identification and distribution in the nervous system and during the formation of synaptic contacts in vitro.the distribution and biochemical features of the synapsin-like peptides recognized in aplysia and helix by various antibodies directed against mammalian synapsins were studied. the peptides can be extracted at low ph and are digested by collagenase; further, they can be phosphorylated by both protein kinase a and ca2+/calmodulin-dependent protein kinase ii. in the ganglia of both snails, they are associated with the soma of most neurons and with the neuropil; punctate immunostaining is present a ...19968996802
blood group glycolipids as epithelial cell receptors for candida albicans.the role of glycosphingolipids as possible epithelial cell receptors for candida albicans was examined by investigating the binding of biotinylated yeasts to lipids extracted from human buccal epithelial cells and separated on thin-layer chromatograms. binding was visualized by the addition of 125i-streptavidin followed by autoradiography. five c. albicans strains thought from earlier work to have a requirement for fucose-containing receptors all bound to the same three components in the lipid e ...19968641797
lectin histochemistry of pregnant rat uterine tissues.glycoconjugate residues were examined in the rat uterus at days 10, 12 and 15 of pregnancy using 17 biotinylated lectins with specificities for a variety of carbohydrate moieties. a wide variety of glycoconjugate residues were detected in the cytoplasm of some antimesometrial and mesometrial decidual cells with lectins from canavalia ensiformis (con a), lens culinaris (lch) culinaris (lch), pisum sativum (psa), griffonia simplicifolia ii (gs-ii), triticum vulgaris (wga), griffonia simplicifolia ...19968655407
binding properties of a blood group le(a+) active sialoglycoprotein, purified from human ovarian cyst, with applied lectins.studies on the structures and binding properties of the glycoproteins, purified from human ovarian cyst fluids, will aid the understanding of the carbohydrate alterations occurring during the biosynthesis of blood group antigens and neoplasm formation. these glycoproteins can also serve as important biological materials to study blood group a, b, h, le(a), le(b), le(x), le(y), t and tn determinants, precursor type i and ii sequences and cold agglutinin i and i epitopes. in this study, the bindin ...19968672551
lectin histochemistry in the rats brain in experimental postresuscitation syndrome. (early and late changes).the morphological and histochemical changes in the rat brain, resulting from global cerebral ischemia due to cardiac arrest and cessation of respiratory function, connected with the disturbances of blood-brain barrier mechanisms inclined us to perform a series of studies on the localization of specific sugar residues in the membrane glycoprotein chains, using lectin techniques. chosen lectins, represented by synthetic plant glycoproteins which are specifically bound to particular sugar residues ...19968791896
generation of in vitro natural cytotoxicity of horse lymphocytes against sarcoid-derived tumor cells not expressing major histocompatibility complex antigens.to analyze in vitro lymphocyte-mediated immune responses of horses with sarcoids against allogeneic sarcoid cells containing endogenous retrovirus but not expressing major histocompatibility complex antigens.19968807009
identification of beta-glucuronidase-resistant diastereomeric glucuronides of 3-hydroxy-3-methyloxindole formed during 3-methylindole metabolism in goats.goats were jugularly infused with the pneumotoxin 3-methylindole (3mi; 15 mg/kg, 0.5 microci/kg) dissolved in cremophor-el to characterize the urinary metabolites of 3mi in a ruminant specie. urine was collected for 36 hr after the beginning of a 2-hr infusion period, and 3mi metabolites were purified using reversed-phase hplc. goats excreted 3mi as at least 11 distinct metabolites. metabolites were characterized using a combination of uv spectroscopy, 1h- and 13c-nmr spectroscopy, and negative- ...19968825199
guinea pig intestinal phospholipase b: protein expression during enterocyte maturation and effects of n-oligosaccharide removal on enzymatic activities and protein stability.guinea pig phospholipase b (plb) is an intestinal brush-border hydrolase displaying a broad substrate specificity towards various dietary lipids. plb was detected by immunoblotting as a single 140-kda polypeptide in all cell populations isolated from guinea pig intestinal mucosa, but increased in parallel to its activity from undifferentiated to mature cells, the specific activity of the enzyme remaining constant. moreover, n-glycosylation, which contributed to 23% of the apparent molecular mass ...19968856041
identification, purification and analysis of a 55 kda lectin binding glycoprotein present in breast cancer tissue.the helix pomatia agglutinin (hpa)-binding glycoproteins from primary breast cancers and their metastases were compared with appropriate normal control tissues on western blots. from these studies a single glycoprotein of 55 kda was found to bind hpa in tumours but not in normal control tissues. the glycoprotein was identified by protein sequencing as being homologous to human immunoglobulin heavy chain variable region. subsequent immunostaining showed it to be immunoglobulin subclass a. iga1 wa ...19968894309
localization of binding sites of ulex europaeus i, helix pomatia and griffonia simplicifolia i-b4 lectins and analysis of their backbone structures by several glycosidases and poly-n-acetyllactosamine-specific lectins in human breast carcinomas.several studies have shown the deletion of blood group a or b antigens and the accumulation of h antigens in human breast carcinomas. other studies have independently demonstrated that the binding sites of lectins such as helix pomatia agglutinin (hpa) and griffonia simplicifolia agglutinin i-b4 (gsai-b4) are highly expressed in these cells. in order to clarify the molecular mechanisms of malignant transformation and metastasis of carcinoma cells, it is important to understand the relationship b ...19968897074
specific effect of maleate on an apical membrane glycoprotein (gp330) in proximal tubule of rat kidneys.maleate treatment of rats induces transport defects similar to those seen in the fanconi syndrome (glycosuria, aminoaciduria, phosphaturia, proteinuria, etc.) and causes an accumulation of apical vesicles in proximal tubule epithelial cells. because the apical membrane glycoprotein, gp330, is a receptor associated with the apical endocytotic and recycling apparatus in these cells, we examined the effect of maleate on the distribution of this protein and other brush border markers. rats received ...19968898022
prediction of neurotoxic potency of hazardous substances with a modular in vitro test battery.neurotoxic action was investigated on different model nervous systems linked to a modular in vitro test battery. voltage operated potassium channels and glutamate operated ion channels expressed in oocytes of the clawed frog xenopus laevis by injection of crna (cloned rna) or mrna, respectively, as well as isolated neurons and isolated neuronal networks from the buccal ganglia of the snail helix pomatia, were used as consecutive modules of different complexity. lead (pb2+) was chosen as a known ...19968920725
lectins and antibodies against blood-group antigens as tools for studying the cellular source of glycoproteins in human bronchial fluid: a comparison of morphological and biochemical observations.we used nine lectins and three antibodies directed against abo blood-group antigens in morphological and western-blot experiments to investigate the source of secretory products of human large airways. in tissue sections, the lectins from griffonia simplicifolia (type i b4), dolichos biflorus, and helix pomatia, and the antibodies to the a, b, and/or h-antigen bound to mucous gland cells and to goblet cells; the binding of these substances was dependent on secretor status and abo blood group. th ...19968929348
distribution of lectin binding sites in human bone marrow. identification by use of an ultrastructural postembedding technique.the purpose of this ultrastructural study was to detect various carbohydrate residues on mature elements of the major human haematopoietic cell lines (granulopoiesis, erythropoiesis and megakaryopoiesis), sinus endothelium and plasma cells under comparable experimental conditions. marrow specimens were processed according to a modified postembedding technique with unicryl as embedding resin. a broad panel of 10 digoxigenin (dig)-conjugated lectins was applied for staining and specificity was eva ...19968933737
the human muc2 mucin apoprotein appears to dimerize before o-glycosylation and shares epitopes with the 'insoluble' mucin of rat small intestine.rabbit antiserum against a synthetic peptide corresponding to a tandemly repeated amino acid sequence in the human intestinal mucin apoprotein muc2 was used in immunoprecipitation to study the biosynthesis of muc2 in the colon-carcinoma cell line ls 174t. under non-reducing conditions, two bands were precipitated, the smaller with an apparent size of about 700 kda on sds/page. when analysed by two-dimensional electrophoresis after reduction, the larger band migrated to the same position as the s ...19958948445
suitability of different staining methods for the identification of isolated and cultured cells from guinea pig (cavia aperea porcellus) stomach.cell suspensions from the guinea pig gastric mucosa were obtained using a pronase/collagenase isolation method, and cultured on petri dishes in minimum essential medium at 37 degrees c. for proper identification of different gastric cell types in cytospots, cell suspensions or culture, selective staining methods were employed, modified and evaluated. mucous cells and mucous neck cells were detected by use of lectins. mucous cells were stained on cytospots and in primary cultures with lectins fro ...19958835136
a new subgroup of lectin-bound biliary proteins binds to cholesterol crystals, modifies crystal morphology, and inhibits cholesterol crystallization.biliary proteins inhibiting or promoting cholesterol crystallization are assumed to play a major role in cholesterol gallstone pathogenesis. we now report a new group of biliary proteins that bind to cholesterol crystals, modify crystal morphology, and inhibit cholesterol crystallization. various glycoprotein mixtures were extracted from abnormal human gallbladder bile using lectin affinity chromatography on concanavalin a, lentil, and helix pomatia columns and were added to supersaturated model ...19958675674
a histological, lectin and s-100 histochemical study of the developing prenatal human sublingual salivary gland.lectin and s-100 protein histochemistry during fetal growth and development (10-38th gestational weeks) of these glands was studied. the histological development of glandular structures followed the known pattern for other salivary glands. using biotinylated lectins ulex europeus-i, dolichos biflorus, glycine maximus (soyabean), helix pomatia, arachis hypogaea (peanut) and triticum vulgare (wheatgerm), the binding level and, by implication, the concentration of associated specific oligosaccharid ...19958670026
glycoconjugates and keratin 18 define subsets of taste cells.sections of neonatal, normal adult and denervated adult rat tongue were examined with lectin histochemistry. attention was focused upon intragemmal cells (cells within the taste bud) and the surrounding perigemmal cells. informative staining patterns were observed with four of 12 lectins: ulex europaeus (uea-i), bauhinia purpurea (bpa), helix pomatia (hpa) and lotus tetragonolobus (lta) agglutinins. in normal adult tongues, bpa bound to those lingual epithelial cells lacking contact with the bas ...19958789401
lectin-binding properties of human breast cancer cell lines and human milk with particular reference to helix pomatia agglutinin.several studies have shown binding of a variety of lectins to breast cancer cells in tissue sections. in particular, binding of the lectin from the roman snail, helix pomatia agglutinin (hpa), to breast cancer cells is linked with a poor prognosis. the molecular basis for lectin binding to metastatic breast cancers is not known. to elucidate this in a model system, lectin-binding patterns of seven human breast cancer cell lines were investigated, their cell membranes were isolated, and hpa bindi ...19957868857
cannabinoids in humans. ii. the influence of three methods of hydrolysis on the concentration of thc and two metabolites in urine.glucuronide conjugates of cannabinoids were previously identified in humans. for gas chromatographic-mass spectrometric (gc-ms) analysis of the unconjugated compounds in human urine, it is necessary to cleave the glucuronide moiety. base hydrolysis and two forms of enzymatic hydrolysis were compared in this study to examine any quantitative differences between the hydrolysis methods. human volunteers (n = 8) each smoked one marijuana cigarette containing 3.58% delta 9-tetrahydrocannabinol (thc) ...19957500615
carbohydrate and peptide antigens in macrophage populations derived from human bone marrow and milk: an immunomorphological and immunochemical analysis.an immunomorphological and immunochemical study was performed to elucidate the pattern of carbohydrate antigens and their relationships to the cluster differentiation (cd) 68 epitopes on macrophages derived from human bone marrow and milk. core and backbone antigens recognized by lectins from bauhinia purpurea (bpa), helix pomatia (hpa), arachis hypogaea (pna), glycine max. (sba), griffonia simplicifolia (gsa-i-b4), lycopersicon esculentum (lea) and erythrina cristagalli (eca) were expressed by ...19958550384
intermediate forms of glycoconjugates are present in the envelope of herpes simplex virions during their transport along the exocytic pathway.in cells infected with herpes simplex virus 1, intracellular virions in transit along the exocytic pathway carry glycoconjugates that react, in fracture-label technique, with helix pomatia lectin. this lectin is specific for unsubstituted n-acetylgalactosamine, an intermediate sugar added in o-linkage to ser/thr residues in cis-golgi and then substituted with galactose and sialic acid in the trans-golgi. virions in the perinuclear space do not react with helix pomatia lectin. in intracellular tr ...19958553564
lectin binding to extracellularly melanized microfilariae of brugia malayi from the hemocoel of anopheles quadrimaculatus.binding patterns of fluorescein isothiocyanate (fitc)- and gold-conjugated lectins to extracellularly melanized sheathed and exsheathed microfilariae of subperiodic brugia malayi, isolated from and in situ in the abdominal hemocoel of anopheles quadrimaculatus 72-hr postinfection, were examined. five fitc-conjugated lectins [helix pomatia agglutinin (hpa), arachis hypogaea (peanut agglutinin-pna), triticum vulgaris (wheat germ agglutinin-wga), lens culinaris (lentil-lch), and concanavalin a (con ...19958568282
growth related changes in sugar determinants on the surface of c6 glioma cells in culture: a cytochemical lectin-binding study.the cell surface sugar determinants (cssd) were examined in c6 glioma cells in cultures at different conditions of growth by peroxidase conjugates of the lectins: peanut agglutinin (pna), ricinus communis agglutinin (rca), helix pomatia agglutinin (hpa), wheat germ agglutinin (wga), lentil agglutinin (lca), laburnum bork agglutinin (laba), and lotus agglutinin (tpa). it was found that the cells bound more intensively wga, lca, and rca compared to pna, hpa; the weakest staining was provided by la ...19958568919
monoclonal antibody recognizing a determinant on type 2 chain blood group a and b oligosaccharides detects oncodevelopmental changes in azoxymethane-induced rat colon tumors and human colon cancer cell lines.altered expression of abh blood group substances is a common feature of human colorectal carcinoma, yet it remains unclear how these structural changes influence the biological properties of tumor cells. azoxymethane-induced rat colon tumors display many features of the human disease, thereby providing a potentially useful model to study the role of blood group substances in colon cancer progression. we have prepared monoclonal antibodies to a microsomal fraction isolated from an azoxymethane-in ...19957536750
lectin and immunohistochemical comparison of glycoconjugates in the conjunctiva of patients with and without exfoliation syndrome.aims--the study was carried out to search for labelling similar to that of intraocular exfoliation material in the conjunctiva by light microscopy using lectin and immunohistochemistry. methods--ten formalin fixed and paraffin embedded conjunctival biopsy specimens both from patients with and without exfoliation syndrome were studied with a panel of 11 lectins and with three monoclonal antibodies to the hnk-1 carbohydrate epitope, all of which react with intraocular exfoliation material. results ...19957542024
comparative effects on biliary concanavalin a-bound glycoproteins and calcium ion on cholesterol crystal nucleation and growth in model bile.the concanavalin a-bound glycoproteins in human gallbladder bile have recently been demonstrated to be strong promoters of cholesterol crystal nucleation. in the present study, we investigated the mechanism(s) whereby such promoters affect cholesterol crystal nucleation and/or growth, and compared these mechanisms with those of another promoter, calcium ion. concanavalin a-bound glycoproteins were isolated from the helix pomatia-unbound fraction of gallbladder bile from stone-free patients, and ...19957550861
comparison of the hydrolysis rates of morphine-3-glucuronide and morphine-6-glucuronide with acid and beta-glucuronidase.the acid and enzymatic hydrolysis rates for two morphine metabolites, morphine-3-glucuronide (m3g) and morphine-6-glucuronide (m6g), were compared. urine fortified with m3g or m6g was hydrolyzed in a boiling water bath or with beta-glucuronidases. optimal temperatures for beta-glucuronidases were 60 degrees c for helix pomatia and abalone and 50 degrees c for escherichia coli. a 3-h incubation with 1,000-1,500 fishman units of enzyme gave complete hydrolysis of m3g. the enzymatic hydrolysis rate ...19957564293
purification of murine pulmonary type ii cells for flow cytometric cell cycle analysis.mice are widely used as animal models for in vivo lung disease. despite this fact, few methods exist for isolation of type ii pneumocytes from mouse lung, limiting the study of alveolar epithelial characteristics in these models. this study investigated several methods for labeling murine lung cell suspensions for flow cytometric identification and sorting of type ii pneumocytes. crude lung cell suspensions were prepared after intratracheal instillation of dispase and were labeled using phosphin ...19957621777
lectins inhibit the aujeszky's disease virus-induced interferon-alpha production of porcine peripheral blood mononuclear cells.the interaction between virus and peripheral blood mononuclear cells (pbmc) required to elicit the production of interferon-alpha (ifn-alpha) by the so-called natural interferon-producing cell is unknown. however, results from inhibition experiments suggest that viral glycoproteins are essential in this ifn induction process. we demonstrate here that cellular glycoproteins also appear to be involved in the initiation of ifn-alpha production. lectins, that is, sugar binding glycoproteins, inhibit ...19957627804
isolation of iga1 from human serum by affinity chromatography using an immobilized extract of the albumin gland of helix pomatia.an extract of the albumin gland of helix pomatia was linked to sepharose-4b and used to prepare iga from group o human serum; immunoelectrophoresis showed that the preparation was free of igg and igm. from studies with specific iga subclass antisera and by comparison with the activity of jacalin-produced material the helix pomatia extract was found to be iga1 specific. the preparation had red cell anti-a, b specificity and was suitable for standardizing and controlling anti-human iga reagents. p ...19957655574
comparison of procedures for measuring the quaternary n-glucuronides of amitriptyline and diphenhydramine in human urine with and without hydrolysis.the activities of beta-glucuronidases from helix pomatia, escherichia coli and rat towards the n-glucuronides of amitriptyline and diphenhydramine were considerably lower than those towards standard substrates. the two n-glucuronides were analysed in urine samples by the following procedures: hplc of the intact conjugate after solid-phase extraction on a cation exchanger cartridge or after direct injection of urine; hplc of the aglycone after hydrolysis with beta-glucuronidase from h. pomatia or ...19957674140
structure and genetic polymorphism of blood group a-active glycosphingolipids of the rat large intestine.study of blood group a- and b-active glycosphingolipid content of the epithelium of the large intestine of 16 strains of inbred rats led to the discovery of two related strains, shr and wky, devoid of a-active glycolipids, whereas all strains expressed b-active glycolipids. this finding evidenced a new a/non-a genetic polymorphism in the rat. blood group a-active glycolipids were isolated from the large intestine of f344 rats and purified by affinity chromatography on immobilized helix pomatia l ...19957696327
identification of a sperm penetration factor in the oviduct of the golden hamster.previously, we found oviductal eggs to be significantly more penetrable and fertilizable in vitro than ovulated eggs collected from the ovarian bursa, while bursal eggs were comparable to mature (unovulated) follicular eggs. incubation of follicular eggs with a soluble eluate of oviductal egg cumulus complexes (cof) increased sperm penetration: the activity was macromolecular, was destroyed at 56 degrees c, and was produced in the oviduct. we now report purification of this oviductal factor that ...19957711179
identification of a novel glycosaminoglycan core-like molecule. ii. alpha-galnac-capped xylosides can be made by many cell types.the accompanying article (manzi, a., salimath, p. v., spiro, r. c., keifer, p. a., and freeze, h. h. (1995) j. biol. chem. 270, 9154-9163) reported the complete structure of a novel molecule made by human melanoma cells incubated with 1 mm 4-methylumbelliferyl-beta xyl (xyl beta mu). the product resembles a common pentasaccharide core region found in chondroitin/dermatan sulfate glycosaminoglycans, except that a terminal alpha-gal-nac residue is found in a location normally occupied by beta-galn ...19957721831
a novel cdna sequence encoding the precursor of the d-amino acid-containing neuropeptide fulicin and multiple alpha-amidated neuropeptides from achatina fulica.fulicin (phe-d-asn-glu-phe-val-nh2) is an endogenous neuropeptide containing a d-amino acid from ganglia of the african giant snail achatina fulica. we have cloned a novel cdna (1,995 nucleotides) encoding a fulicin precursor from the snail cerebral and subesophageal ganglia. the fulicin precursor protein (357 amino acids) contains one copy of fulicin and at least nine other putative alpha-amidated neuropeptides composed of four to six amino acid residues. seven of the nine neuropeptides were no ...19957722509
native and asialo-tamm-horsfall glycoproteins as important ligands for the detection of galnac beta 1-->and gal beta 1-->4glcnac active lectins.the binding properties of human tamm-horsfall sd(a+) urinary glycoprotein(thgp) and asialo-thgp with various applied lectins was investigated by quantitative precipitin and precipitin inhibition assays. both glycoproteins completely precipitated abrus precatorius agglutinin(apa). they also reacted well with wistaria floribunda (wfa), glycine max (soybean, sba), and ricinus communis agglutinins and precipitated over 78% of the lectin nitrogen added, but reacted poorly or weakly with all alpha-ano ...19957726822
ultrastructural localization of helix pomatia agglutinin (hpa)-binding sites in human breast cancer cell lines and characterization of hpa-binding glycoproteins by western blotting.ultrastructurally, cells of five human breast cancer cell lines (mcf7, bt549, bt20, t47d, and hbl100) generally displayed many characteristics of their epithelial origin. the most distinctive features were observed in mcf7 cells, which consistently showed microvilli and submembranous granules. these ultrastructural findings served as a basis for localizing the binding sites of the lectin helix pomatia agglutinin (hpa). after use of the pre-embedding method consistent hpa labeling of the cell mem ...19957770962
histochemical study of lectin binding in the human fetal minor salivary glands.the emerging synthesis of glycoconjugates containing specific oligosaccharides in developing human fetal labial and lingual salivary glands has been investigated by lectin histochemistry. an avidin-biotin technique was used to study the binding of lectins from ulex europeus i (uea-i), dolichos biflorus (dba), glycine maximus (sba), helix pomatia (hpa), arachis hypogaea (pna) and triticum vulgare (wga) to specific sugars on sections of tissue from labial glands, glands of blandin and nuhn, glands ...19957776265
use of sialylated or sulfated derivatives and acrylamide copolymers of gal beta 1,3galnac alpha- and galnac alpha- to determine the specificities of blood group t- and tn-specific lectins and the copolymers to measure anti-t and anti-tn antibody levels in cancer patients.sialylated or sulfated derivatives and acrylamide copolymers of blood group t-(gal beta 1,3galnac alpha-) and tn-(galnac alpha) haptens were studied for their interaction with the lectins of peanut (pna), agaricus bisporus-(aba), helix pomatia-(hpa) and vicia villosa b4-(vva), using asialo cowper's gland mucin (acgm), which contains both t and tn epitopes, as the coating substrate in enzyme linked lectin assay. both t and tn copolymers (-40 haptens) showed high affinity and strict specificity; a ...19957795413
distribution of catch-relaxing peptide (carp)-like immunoreactive neurons in the central and peripheral nervous system of helix pomatia.immunocytochemistry was performed on the nervous system of helix by the use of an antibody raised against a myotropic neuropeptide, the catch-relaxing peptide (carp), isolated from mytilus edulis. in each ganglion of the central nervous system of helix pomatia, numerous carp-immunoreactive cell bodies and a dense immunoreactive fiber system could be observed with a dominancy in the cerebral and pedal ganglia. the majority of the immunoreactive neurons are unipolar, although multipolar neurons al ...19957781031
neurons with different immunoreactivity form clusters in the cns of helix pomatia.the distribution of serotonin (5ht-ir), fmrf amide (fmrf a-ir), catch-relaxing-peptide (carp-ir), dopamine (da-ir), gamma-amino-butyric-acid (gaba-ir), and leucokinin (lk-ir) immunoreactive neurons were compared in the ganglia of helix cns. these neurons are not distributed randomly, but their location outlines distinct groups in the ganglia. in a few groups only da-ir, gaba-ir and lk-ir neurons can be seen, whereas in the majority of groups fmrfa-ir, carp-ir and 5ht-ir neurons are localized tog ...19958853698
tachykinin- and leucokinin-related peptides in the molluscan nervous system.distribution of locustatachykinin-like immunoreactive (lomtkli) and leucokinin like immunoreactive (lkli) neurons in the central nervous system (cns) of the pond snail, lymnaea stagnalis was investigated and compared to that found in helix pomatia. occurrence of lomtkli neurons in different ganglia of the freshwater bivalve, anodonta cygnea, was also studied. similar to helix, the lymnaea cns contained lomtkli and lkli neurons mainly in the cerebral and pedal ganglia, but the number of labelled ...19958853699
detection of dihydrotestosterone (dht) doping: alterations in the steroid profile and reference ranges for dht and its 5 alpha-metabolites.dihydrotestosterone (dht), a biologically active metabolite of testosterone, may be misused in sports to benefit from its anabolic and psychotropic effects. after dht application, a significant increase of the glucuronides of dht and its metabolites can be expected for a certain time period depending upon dose, formulation, route of administration, and in case of percutaneous administration the chainlength of the ester. dht and its metabolites can be monitored by gas-chromatography/mass spectrom ...19958776070
interaction of hamster submaxillary sialyl-tn and tn glycoproteins with gal, galnac and glcnac specific lectins.hamster submaxillary glycoprotein (hsm), one of the simplest glycoproteins among mammalian salivary mucins, is composed of approximately equivalent amounts of protein, hexosamine and sialic acid. the thr and ser residues in the protein core account for more than half of all of the amino acid residues, while lys, glu, pro and ala are the major components of the remaining portion of amino acids. the carbohydrate side chains of this mucous glycoprotein have mainly the neuac-galnac-(sialyl-tn) seque ...19948183285
interaction of a novel tn (galnac alpha 1-->ser/thr) glycoprotein with gal, galnac and glcnac specific lectins.a naturally occurring tn glycoprotein (native asg-tn) with galnac alpha 1-->ser/thr as the only carbohydrate side chains, has been prepared from armadillo submandibular glands. in a quantitative precipitin assay, this glycoprotein completely precipitated maclura pomifera (mpa), vicia villosa b4 (vvl-b4) and artocarpus integrifolia (jacalin, ail). it also reacted well with helix pomatia (hpl) and wistaria floribunda (wfl) and precipitated over 75% of the lectin nitrogen added, but poorly with ric ...19948292029
nm23 expression versus helix-pomatia lectin-binding in human breast-cancer metastases.the relationship between nm23 expression, hpa staining, and other clinicopathological variables, i.e., axillary (ax) and internal mammary lymph node (imn) metastases was assessed, and their prognostic values were analyzed. positive hpa staining was found to be significantly correlated with tumor size, and with ax and imn metastases, whereas negative nm23 expression was significantly correlated with ax and imn metastases. a univariate study revealed that overall survival was correlated significan ...199421567061
[identification of group c streptococcus with helix pomatia lectin in use for blood grouping].the lectin anti-ahp (helix lectin) obtained from the albumen gland of an edible snail, helix pomatia, was used for the identification of group c streptococci. all 1,045 strains of group c streptococci (s. equisimilis, s. zooepidemicus, and s. dysgalactiae) were agglutinated with the helix lectin, but none of the 12,264 strains of group a, 1,346 strains of group g, 330 strains of group b, and 121 strains of other streptococcal groups was not. a few strains of s. equisimilis which belongs to group ...19947830321
biliary proteins from hepatic bile of rats fed curcumin or capsaicin inhibit cholesterol crystal nucleation in supersaturated model bile.following our earlier observations that curcumin and capsaicin are antilithogenic in mice and hamsters, attempts were now made to understand the manner in which these spice principles were acting. for this purpose, the hepatic biles of rats fed a control, lithogenic, and lithogenic diet supplemented with curcumin or capsaicin were subjected to gel filtration chromatography (sepharose-4b-cl) and the lmw protein fractions were tested for their ability to influence cholesterol crystal growth in mod ...19947851942
optimal enzymatic hydrolysis of urinary benzodiazepine conjugates.conditions for the enzymatic hydrolysis of benzodiazepine conjugates were systematically examined. optimal recovery of the free drugs occurs when 1 ml of urine buffered to ph 4.5 is incubated with 5000 u of helix pomatia beta-glucuronidase at 56 degrees c for 2 h. urine specimens containing conjugates of temazepam, oxazepam, lorazepam, alpha-hydroxyalprazolam, 2-hydroxyethylflurazepam, and n-desalkyl-3-hydroxyflurazepam as targeted benzodiazepines were used. the freed drugs were quantitated by g ...19947861750
studies on steroids in fetuses and neonates: identification of 16-dehydropregnenolone in the circulation of pre-term neonates.unconjugated steroids were extracted with dichloromethane from the serum of pre-term neonates. conjugated steroid fraction in the residue was separated and purified by high performance liquid chromatography. the purified steroid fraction was subjected to enzyme (arylsulfatase from helix pomatia) or acid hydrolysis. liberated steroid was analyzed by gas chromatography-mass spectrometry (gc/ms) and gas chromatography-fourier transformation infrared spectroscopy (gc/ft-ir). all of the results obtai ...19947703972
histochemical characteristics of the goblet cell mucins and their role in defence mechanisms against nippostrongylus brasiliensis infection in the small intestine of mice.the histochemical characteristics of the small intestinal goblet cell mucins of mice and their protective role during the expulsion of nippostrongylus brasiliensis adult worms were examined. lectin histochemistry revealed that, before infection, strongly positive stainability of helix pomatia agglutinin (hpa) and limax flavus agglutinin (lfa) was observed in all of the small intestinal goblet cell mucins of mice. when 'normal' or 'damaged' n. brasiliensis adult worms were implanted separately in ...19947708431
growth hormone regulates an n-acetylgalactosamine component in odontogenesis: a specific lectin-binding study in the lewis dwarf rat.dental organs of incisors from normal, dwarf and growth hormone-treated dwarf rats were analysed histochemically using a panel of lectins. a distinctive pattern of differential staining was obtained with helix pomatia agglutinin, a lectin specific for n-acetylgalactosamine. in bouin's perfused and paraffin-embedded undecalcified tissues from normal rats, reaction product for n-acetylgalactosamine was visible in the odontogenic cells and some extracellular matrices. in the growth hormone-deficien ...19947519266
radioimmunoassay of 2-hydroxyestrone in urine.catechol estrogens such as 2-oh estrone are interesting estrogen metabolites formed in several human tissues and excreted in urine. we developed and thoroughly validated a radioimmunoassay for urinary 2-oh estrone that has several advantages over published rias. because we developed a relatively specific antiserum, we did not include a preliminary chromatographic step to eliminate cross-reacting urinary steroids. we hydrolyzed urinary steroid conjugates with beta-glucuronidase from helix pomatia ...19948287549
characterization of the budding compartment of mouse hepatitis virus: evidence that transport from the rer to the golgi complex requires only one vesicular transport step.mouse hepatitis coronavirus (mhv) buds into pleomorphic membrane structures with features expected of the intermediate compartment between the er and the golgi complex. here, we characterize the mhv budding compartment in more detail in mouse l cells using streptolysin o (slo) permeabilization which allowed us to better visualize the membrane structures at the er-golgi boundary. the mhv budding compartment shares membrane continuities with the rough er as well as with cisternal elements on one s ...19948294506
heterogeneity of human gallbladder mucin in bile.1. human gallbladder mucin has been implicated to play a role in gallstone disease. in spite of this fact relatively little is known about the structure of human gallbladder mucin. in this study we have investigated the possible heterogeneity of mucin. for this purpose polyclonal and monoclonal antibodies against gallbladder mucin were raised. all antibodies reacted primarily with carbohydrate antigenic determinants. with these antibodies the immunoreactivity of gallbladder mucin from 60 patient ...19948306554
[the human spleen in idiopathic thrombocytopenic purpura].idiopathic (autoimmune) thrombocytopenic purpura (itp, aitp) represents a relatively frequent impairment. it involves a syndrome of various diseases with a shortened thrombocytes survival caused by anti-platelet antibodies. the majority of cases are of secondary character. spleenectomy often evokes a complete remission of thrombocytopenia. the study describes morphologic findings in spleens of 30 patients with the clinical diagnosis of itp/aitp. the findings were gained by light microscopy from ...19947882065
modes of entry of the first-stage larvae of elaphostrongylus cervi (nematoda: protostrongylidae) into pulmonate snails arianta arbustorum and helix pomatia.experimental infection of the pulmonate snails arianta arbustorum l. and helix pomatia l. with first-stage larvae of protostrongylid nematode elaphostrongylus cervi cameron, 1931 was performed in order to determine modes of larval entry into the body of the snail intermediate host. groups by four individuals of both snail species were examined histologically 30, 60, 90, and 120 minutes after the beginning of exposure and 1, 2, 4, and 7 days post infection. all 64 snails examined were found to be ...19947883253
lectin binding in the anterior segment of the bovine eye.eleven different fluorescent lectin-conjugates were used to reveal the location of carbohydrate residues in frozen sections of the anterior segment of bovine eyes. the lectins were specific for the following five major carbohydrate groups: (1) glucose/mannose group (concanavalin a (con a)); (2) n-acetylglucosamine group (wheat germ agglutinin (wga)); (3) galactose/n-acetylgalactosamine group (dolichos biflorus agglutinin (dba), helix pomatia agglutinin (hpa), helix aspersa agglutinin (haa), psop ...19947883589
interactions with lectins and agglutination profiles of clinical, food, and environmental isolates of listeria.on the basis of preliminary trials with 14 collection strains of listeria, five lectins (canavalia ensiformis, concanavalin a; griffonia simplicifolia lectin i; helix pomatia agglutinin; ricinus communis agglutinin; and triticum vulgaris wheat germ agglutinin) were selected to set up a microtiter agglutination assay. the lectin agglutination profiles of 174 clinical, food, and environmental strains of listeria monocytogenes, listeria innocua, and listeria seeligeri were investigated. data on the ...19947883879
hyperplasia, hypertrophy, and phenotypic alterations in the distal nephron after acute proximal tubular injury in the rat.little is known about the impact of acute proximal tubular injury and dysfunction on the distal nephron.19947909858
glycosylation patterns of the human colon cancer cell line ht-29 detected by helix pomatia agglutinin and other lectins in culture, in primary tumours and in metastases in scid mice.human colonic cancer cells (ht-29, 10(7) cells/dose) were injected subcutaneously between the scapulae of 19 severe combined immunodeficient (scid) mice. after 19 days, large tumours had developed in 18 out of the 19 animals and the mice were then killed. metastases were detected in the lungs of 16 animals but not in other organs investigated. surgical removal of the primary tumour in another group of five animals led to a prolonged survival and further growth of metastases in the lungs. ht-29 i ...19947923992
role of cell surface o-linked oligosaccharides in adhesion of hl60 cells to fibronectin: regulation of integrin-dependent cell adhesion by o-linked oligosaccharide elongation.the adhesion of the myelogenous leukemia cell line, hl60, to fibronectin and its fragments, heparin binding fragment (40 kda) and cell attachment fragment (120 kda), was enhanced by culturing with benzyl-alpha-galnac (bz alpha galnac). enhancement of cell adhesion to fibronectin was also observed on treatment of hl60 cells with 12-o-tetradecanoylphorbol 13-acetate (tpa). however, an additive effect of bz alpha galnac and tpa treatments was not observed. the expression of vla4 and vla5 did not ch ...19947925647
comparative aspects of neoplastic invasion of the brain.this review of the invasion of the human brain by tumors is based upon a comparative approach. the taxonomic distribution of a structure of the cns to be considered a brain is present in the following taxonomic groups: mollusca (cephalopoda, pulmonata) sipunculida, echiurida, annelida, arthropoda and vertebrata (fishes, amphibians, reptiles, birds and mammals). the comparative approach (inter-, and intraspecies specific) provides a more informative, indicative, understanding of invasion of the h ...19947964978
helix pomatia agglutinin binding is a useful prognostic indicator in colorectal carcinoma.most deaths from colorectal carcinoma are due to metastases. a relatively reliable prognostic indicator at surgery to date is the dukes' stage, but this is a morphologic approach that does not elucidate biochemical changes to explain why cells became metastatic. the binding sites for the lectin from the roman snail helix pomatia (hpa) were shown to be good prognostic indicators in breast and gastric cancer, and accordingly, this study was performed to evaluate the use of hpa binding sites as pro ...19947982174
galactose-containing glycoconjugates of the ciliary body and lens in capsular glaucoma: a lectin histochemical study.this study was carried out in order to obtain information on galactose-containing glycoconjugates in the lens and ciliary body of human eyes with capsular glaucoma and thus on the etiopathogenesis of exfoliation syndrome.19948001826
differential lectin binding of onchocerca lienalis and onchocerca ochengi infective larvae following their development in simulium ornatum s.l.lectins have been used to investigate species specific differences in carbohydrate moieties on the surface of the infective larvae of two onchocerca species following their development in simulium ornatum. of the seven fitc-labelled lectins used in this study only two, arachis hypogea (pna) and helix pomatia (hpa), bound to the surface of o. lienalis, whereas no lectin binding could be detected on the surface of o. ochengi infective larvae. this indicates that in principal lectins might be a pot ...19948066375
alpha-n-acetylgalactosaminidase deficiency with mild clinical manifestations and difficult biochemical diagnosis.two additional patients with alpha-n-acetylgalactosaminidase (alpha-naga) deficiency are described. an 11-month-old girl with nonconsanguineous parents had generalized seizures and no angiokeratoma. biochemical investigation showed persistent slight oligosacchariduria; enzymatic analysis of plasma, leukocytes, and fibroblasts revealed profound alpha-naga deficiency. heterozygote enzyme levels were found in both parents. the mother has epilepsy, and epilepsy is present in the father's family. a y ...19948071745
immunocytochemical distribution of neuropeptide f (npf) in the gastropod mollusc, helix aspersa, and in several other invertebrates.the distribution of neuropeptide f (npf) immunoreactivity in the snail, helix aspersa, has been demonstrated by immunocytochemistry using 2 region-specific antisera. one, designated npf3, was raised against a synthetic n-terminal fragment of helix aspersa npf; the other, designated pp221, was raised against the c-terminal hexapeptide amide of mammalian pancreatic polypeptide (pp) but cross-reacts fully with the analogous c-terminal region of helix aspersa npf. the distribution of npf immunoreact ...19948111844
identification of a major poly-n-acetyllactosamine-containing cell-surface glycoprotein of mouse teratocarcinoma cells. appearance on cells induced to primitive endoderm but not parietal endoderm differentiation.mouse teratocarcinoma f9 cells were induced to primitive endoderm differentiation with retinoic acid, and poly-n-acetyllactosamine-containing surface glycoproteins were identified by radiolabelling endo-beta-galactosidase-cleavable glycans with galactosyltransferase and radiolabelled udp-galactose. one major radiolabelled band with an apparent size of 250-500 kda was identified which differed from the known poly-n-acetyllactosamine-containing glycoproteins laminin, fibronectin, lysosome-associat ...19948125095
helix pomatia agglutinin binding in human tumour cell lines: correlation with pulmonary metastases in nude mice.the extent of lectin binding by three human melanoma (lox, femx-1 and sesx) and two sarcoma lines (mhmx and ohsx) was related to their potential for experimental metastasis formation in athymic nude mice. the helix pomatia agglutinin (hpa), which recognises the n-acetyl-d-galactosamine ligand, showed differential binding to the cell lines in a manner that correlated with their ability to give lung colonies after i.v. injection in the mice (p < 0.005). the degree of hpa binding and lung colony fo ...19948198963
carbohydrate antigens of human megakaryocytes and platelet glycoproteins: a comparative study.until now, carbohydrate antigens of human megakaryocytes have not been studied very extensively. for this reason, we investigated the staining pattern of 25 lectins and carbohydrate-specific monoclonal antibodies on paraffin-embedded trephine biopsies and acetone-fixed smears from patients with reactive and neoplastic bone marrow lesions. a biotin-streptavidin-alkaline phosphatase assay was used to visualize the binding of lectins or antibodies. ulex europaeus agglutinin i (uea-i) stained megaka ...19947532635
the use of lectins to characterize and separate living canine spermatozoa: a preliminary study.this study was designed as a preliminary attempt to develop a methodology for relating the glucidic structure of the sperm membrane to sperm morphology. differences in plasma membrane glycoconjugates between motile and nonmotile spermatozoa were studied by using 7 lectins. fresh spermatozoa from 3 dogs were analyzed by fluorescein isothiocyanate (fitc) labeled lectins. the binding of lectins to the sperm membrane and the capability of the lectins to agglutinate spermatozoa were estimated semi-qu ...199416727393
[long term survival of first stage larvae of elaphostrongylus cervi (nematoda, metastrongyloidea) in a frozen state].faeces of red deer from białowieza forest containing first stage larvae of elaphostrongylus cervi have been stored during five years in -20 degrees c. after this time 52% of the larvae survived. these larvae were fully alive and in experimentally infected snails helix pomatia developed to the infectious stage.19938237006
differentiation of pancreatic acinar cells into duct-like cells in vitro.previous studies have shown that a combination of both the extracellular matrix and secretagogues plays critical roles in the maintenance of well-differentiated pancreatic acinar cells in culture. in the present study, we have shown that, upon proper stimulation, acinar cells change their growth pattern and morphologic appearance to a duct like phenotype.19938246444
histochemistry with helix pomatia agglutinin in human germ cell tumors: detection of nongerminomatous components and correlation between hpa reactivity and radiosensitivity in germinomas.binding sites of helix pomatia agglutinin (hpa) were examined in 32 patients with intracranial human germ cell tumors. hpa reactivity was found in vascular endothelial cells and erythrocytes of patients with blood type a or ab. hpa-positive neoplastic cells were seen in one yolk sac carcinoma in a patient with blood group a, and in embryonal carcinomas and teratomas irrespective of blood group type. although in 10 out of 18 germinomas neoplastic cells were totally negative for hpa, another 8 ger ...19938252515
developmental changes in neutral glycosphingolipids of mouse placenta.the mammalian placenta is a unique organ for the study of developmental changes. placentas of laboratory animals such as the mouse allow for the determination of the exact stage of pregnancy, which cannot be achieved with human placenta. in this study, neutral glycosphingolipids were isolated from mouse (inbred strain c57bl/6) placentas, from day 10 to day 18 of gestation, and were separated by high performance thin layer chromatography. densitometric measurements after orcinol staining showed, ...19938257854
affinity methods with lectins: a tool to identify canine alkaline phosphatase isoenzymes.affinity methods were used to characterize selective interactions of alkaline phosphatase (alp) isoenzymes from different dog tissues with lectins. specific lectins were used to identify liver, intestinal and steroid-induced alp isoenzymes in serum from dogs with cushing syndrome or steroid-treated dogs. for the first approach, 12 lectins were assayed by affinity dots. selective interactions were found among wheat germ agglutinin (wga), jacalin, con a (concanavalin a) and helix pomatia agglutini ...19938258637
histochemical analysis of rat testicular glycoconjugates. 3. non-reducing terminal residues in seminiferous tubules.lectins of helix pomatia (hpa), glycine max (sba), vicia villosa (vva), dolichos biflorus (dba), ulex europaeus (uea-1), tetragonolobus purpureus (lta), griffonia simplicifolia (bsa-1b4), maclura pomifera (mpa), sambucus nigra (sna) and maackia amurensis (maa) were used to explore the distribution of saccharides characteristic of non-reducing termini of o- and n-linked glycoprotein glycans in the seminiferous tubules of rat testis. sialyl residues (both alpha 2,3- and alpha 2,6-linked, as shown ...19938282567
prognostic significance of helix pomatia lectin and c-erbb-2 oncoprotein in human breast cancer.we investigated the prognostic significance of helix pomatia lectin (hpa) staining on disease-free and overall survival in 120 primary breast carcinomas. hpa staining was present in 58 (48%) of these carcinomas. it was significantly associated with axillary lymph node metastases (p < 0.001) and c-erbb-2 expression (p < 0.01). a univariate study revealed that disease-free and overall survival were significantly correlated with clinical stage, tumour size, axillary lymph node metastases. hpa stain ...19938102537
lectin histochemistry of normal and neoplastic peripheral nerve sheath. 1. lectin binding pattern of normal peripheral nerve in man.the binding patterns of lectins to normal peripheral nerves were examined. twelve biotinylated lectins were used in this study; canavalia ensiformis (con a), pisum sativum (psa), lens culinaris (lca), ricinus communis 1 (rca-1), arachis hypogaea (pna), glycine max (sba), sophora japonica (sja), bandeiraea simplicifolia 1 (bsl-1), triticum vulgaris (wga), succinylated wga (s-wga), ulex europaeus 1 (uea-1) and helix pomatia (hpa). cytoplasm of schwann cells and perineurial cells was stained by con ...19938310810
the use of lectins identified with specific antibodies in lectin histochemistry of nzb/w f1 mouse kidney.the affinity of helix pomatia, peanut, pisum sativum, soy bean, and wheat germ agglutinins to various nephron parts of nzb/w f1 mice was different and is assumed to be age dependent. the affinity of pisum sativum agglutinin to basal membranes of small renal vessels increased with the age of nzb/w f1 mice. the wheat germ agglutinin bound to structures with alkaline phosphatase activity.19938351979
neurons in a variety of molluscs react to antibodies raised against the vd1/rpd2 alpha-neuropeptide of the pond snail lymnaea stagnalis.the vd1 and rpd2 neurons of lymnaea stagnalis innervate other central neurons, certain skin areas, the pneumostome area, and the auricle of the heart. recently, a set of four (delta, epsilon, alpha, beta) neuropeptides produced by these giant neurons and by certain other central neurons has been characterized. although alternative splicing of the preprohormone of these neurons yields at least 10 different alpha neuropeptides, an affinity-purified antiserum directed against a domain common to all ...19938364974
fragmentation and dispersal of golgi proteins and redistribution of glycoproteins and glycolipids processed through the golgi apparatus after infection with herpes simplex virus 1.in vero monkey cells and hep-2 human epidermoid carcinoma cells infected with herpes simplex virus 1 the proteins beta-cop, galactosyltransferase, and alpha-mannosidase ii associated with the golgi apparatus appear to be associated with numerous smaller structures dispersed throughout the cytoplasm. concomitantly, the intracytoplasmic ligands of lectins normally associated wholly (helix pomatia or ricinus communis agglutinin) or in part (wheat germ agglutinin) with the golgi apparatus increased ...19938385343
immune-mediated alteration of the terminal sugars of goblet cell mucins in the small intestine of nippostrongylus brasiliensis-infected rats.alteration of terminal sugars of goblet cell mucins in the small intestinal mucosa was examined by lectin histochemistry in rats infected with nippostrongylus brasiliensis. to see whether alteration of the nature of mucins was regulated by the local immune system, adult worms were implanted intraduodenally into recipient rats of various immune status. when 7-day-old ('normal') adult worms were implanted into naive euthymic rats, about 60% remained until day 7 and then were expelled. the number o ...19938473019
cytochemical characterization of basement membranes in the enamel organ of the rat incisor.ameloblasts are unique epithelial cells, in that once they have deposited the entire thickness of enamel and the process of maturation begins, they reform a basal lamina-like structure at their apical surface. in order to characterize further this basal lamina, its composition was analysed using (1) lectin-gold cytochemistry for glycoconjugates, (2) high-iron diamine (hid) staining for sulfated glycoconjugates and (3) immunogold labeling for collagen type iv and laminin. the labeling patterns we ...19938500995
intracisternal crystals in pancreatic acinar cells: failure in the distinct aggregation of secretory proteins.mechanisms leading to the formation of crystalline inclusions in the cisternal space of the rough endoplasmic reticulum are poorly understood. this phenomenon was investigated in pancreatic acinar cells using two different experimental models: 1) intraperitoneal injection of dl-p-chlorophenylalanine methyl ester, and 2) culture of isolated acinar cells within the matrigel basement membrane in the presence of 2% dimethyl sulfoxide. features and composition of induced crystals were analyzed by pro ...19937523126
lectin histochemistry of normal and neoplastic peripheral nerve sheath. 2. lectin binding patterns of schwannoma and neurofibroma.lectin binding patterns of 31 schwannomas and 6 neurofibromas were examined using 12 lectins, and the results were compared with those of normal peripheral nerves. tumors obtained from 10 cases of neurofibromatosis and 4 recurrent schwannomas were included. changes of glycoconjugates were observed in association with a neoplastic transformation of schwann cells; arachis hypogaea (pna) staining after neuraminidase treatment seen in normal schwann cells was reduced in schwannoma of antoni type a, ...19938310811
developmental changes in the distribution of cecal lectin-binding sites of balb-c mice.the existence of lectin-binding sites was investigated in the cecum of balb-c mice at seven developmental stages ranging from 18 days post conception (p.c.) to 8 weeks after birth. nine horseradish-peroxidase-conjugated lectins (concanavalin a, triticum vulgaris, dolichus biflorus, helix pomatia, arachis hypogaea, glycine maximus, lotus tetragonolobus, ulex europaeus, limulus polyphemus) were applied to 5- to 7-microns thin paraffin sections of bouin-fixed tissue. after dab staining the sections ...199210457768
ultrastructural, biochemical and electrophysiological changes induced by 5,6-dihydroxytryptamine in the cns of the snail helix pomatia l.the serotonin neurotoxin, 5,6-dihydroxytryptamine (5,6-dht), was injected into the body cavity of snails. changes induced in the central nervous system (cns) by the neurotoxin were studied by morphological, electrophysiological and biochemical techniques for up to 90 days following injection. the neurotoxin induced a variety of ultrastructural alterations during the early phase (1st to 6th days) following treatment. on day 6 after treatment, membranous structures first appeared in the synaptic-l ...19921324760
modulation of voltage-activated ion currents on identified neurons of helix pomatia l. by interleukin-1.1. the effect of interleukin-1 (il-1) was studied on voltage-activated ion currents of the identified central neurons of helix pomatia l. using a two-microelectrode voltage clamp. the voltage-activated inward current (ica) was decreased, whereas the outward current (i(net) k) was increased by il-1. 2. il-1 affects both the transient and the delayed rectifying potassium currents. the il-1 modulatory effect on the voltage-activated ion currents was voltage and dose dependent. the threshold concent ...19921281756
comparative study of prostatic carcinoma bone metastasis among japanese in japan and japanese americans and whites in hawaii.metastatic prostate carcinomas in autopsy cases from three populations 49 cases of indigenous japanese, 29 cases of japanese americans and 14 from whites in hawaii) were compared in terms of their clinicopathological, immunohistochemical (tenascin and ras p21) and lectin binding (helix pomatia antigen, hpa) properties. only the clinicopathological features were analyzed in the cases of whites in hawaii. the results indicate that poorly differentiated carcinoma is less common, whereas distant met ...19921283503
lectin histochemistry and alkaline phosphatase activity in the pia mater vessels of spontaneously hypertensive rats (shr).some lectins were used to study the localization of sugar residues on the endothelial cell surface in the pia mater blood vessels of control (wky) and hypertensive rats (shr). the lectins tested recognized the following residues: beta-d-galactosyl (ricinus communis agglutinin 120, rca-1), alpha-l-fucosyl (ulex europaeus agglutinin, uea-1), n-acetylglucosaminyl and sialyl (wheat germ agglutinin, wga), n-glycolyl-neuraminic acid (limax flavus agglutinin, lfa), and n-acetyl-d-galactosaminyl (helix ...19921297933
early increase in lymphocyte cyclic nucleotide phosphodiesterase activity upon mitogenic activation of human peripheral blood mononuclear cells.cyclic nucleotide phosphodiesterase activity of human peripheral blood mononuclear cells was significantly increased following a short (30 min) incubation with the mitogenic lectin concanavalin a. con a stimulated phosphodiesterase activity to the same extent whatever the subcellular compartment (homogenate, cytosol or pellet). further separation of the con a-activated mononuclear cells into lymphocyte-enriched and monocyte-enriched populations showed that the con a-induced increase of phosphodi ...19921309823
duck lymphocytes--v. transformation responses to phorbol ester and calcium ionophore.1. lymphocytes purified from duck blood and spleen were cultured in the presence of phorbol 12-myristate 13-acetate (pma) and the calcium ionophore a23187. stimulation was assessed by the incorporation of [3h]thymidine after 3 days' culture. 2. pma stimulated over a wide range of concentrations, with maximum stimulation at final concentrations of 5 x 10(-7)-5 x 10(-8) m/litre. a23187 was effective in the range 5 x 10(-6)-5 x 10(-7) m/litre and also, in some experiments using spleen lymphocytes, ...19921354147
quantitative changes of ricinus communis agglutinin i and helix pomatia lectin binding sites in the acrosome of rat spermatozoa during epididymal transit.during passage through the epididymis, spermatozoa undergo a number of changes which result in their acquisition of fertility and motility. some of the changes that occur include loss of the cytoplasmic droplet and changes in sperm morphology, metabolism and properties of the nucleus and plasma membrane. changes have also been reported in the acrosomic system of mammalian spermatozoa during their transit through the epididymis. in the present study, the quantitative changes of the glycoconjugate ...19921385371
mannose-induced dysmorphogenesis of metanephric kidney. role of proteoglycans and adenosine triphosphate.because various fetal anomalies are seen in diabetic offspring, we examined the effects of sugars on proteoglycans (pgs): extracellular matrix (ecm) macromolecules modulating morphogenesis. 13-d-old mouse metanephric kidney explants were exposed to mannose for 7 d and labeled with [35s]sulfate, [35s]-methionine, or [3h]thymidine. mannose exposure caused reduction in kidney size and disorganization of ureteric bud branches with inhibition of glomerulogenesis. tissue autoradiographic and immunoflu ...19921401058
expression of glycoconjugates in the human fetal cochlea.the distribution of glycoconjugates in the human fetal cochlea was analyzed using six biotinylated lectins: wheat germ agglutinin (wga), abrus precatorius agglutinin (apa), ulex europaeus agglutinin i (uea-i), ricinus communis agglutinin 120 (rca120), helix pomatia agglutinin (hpa), concanavalin a (cona). the tectorial membrane (tm) in the 11- and 15-week-old human fetuses was labelled with wga, apa, rca120 and cona, but not with uea-i and hpa. in the 19-week-old fetuses, the reaction of the tm ...19921442009
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