Publications

transformation by agrobacterium rhizogenes and regeneration of transgenic shoots of the wild soybean glycine argyrea.	glycine argyrea accession g1420 was evaluated for its response to inoculation with agrobacterium rhizogenes strains lba9402 and a4t, carrying wild type ri plasmids, and by strains r1601 and a4tiii with engineered plasmids. hypocotyls from young seedlings were the most responsive in producing roots at inoculation sites. root production was also dependent on bacterial concentration. excised, cultured roots produced green nodular callus which regenerated shoots on sc2 medium containing 1.1 mg l(-1) ...	1991	24221492
rflp mapping in soybean: association between marker loci and variation in quantitative traits.	we have constructed a genetic map for soybean and identified associations between genetic markers and quantitative trait loci. one-hundred-fifty restriction fragment length polymorphisms (rflps) were used to identify genetic linkages in an f2 segregating population from an interspecific cross (glycine max x glycine soja). twenty-six genetic linkage groups containing ca. 1200 recombination units are reported. progeny-testing of f2-derived families allowed quantitative traits to be evaluated in re ...	1990	1979039
genetic analysis of soybean hard seededness with molecular markers.	hard seededness in soybean [glycine max (l.) merr.] is a quantitative trait that affects the germination rate, viability, and quality of stored seeds. we have used 72 restriction fragment length polymorphisms (rflps) to identify genomic regions containing quantitative trait loci (qtl) affecting hard seededness in a segregating population from a g. max by a glycine soja (sieb. & zucc.) cross. five independent rflp markers were found to be associated with variation in the hard-seeded trait. these ...	1990	24226449
an attempt to elucidate the origin of cultivated soybean via comparison of nucleotide sequences encoding glycinin b4 polypeptide of cultivated soybean, glycine max, and its presumed wild progenitor, glycine soja.	nucleotide sequences of cdnas encoding soybean glycinin b4 polypeptide were compared for three soybean cultivars and two introductions of wild soybean, g. soja. for three g. max cultivars, only two nucleotide substitutions were found, while g. max and g. soja nucleotide sequences had four substitutions. these data give added proof that g. max originated from g. soja. on the other hand, the time required for the accumulation of four nucleotide substitutions (calculated from the parameters of 11s ...	1989	24226018
[nucleotide sequences encoding glycinin b4 polypeptide of the cultivated soybean glycine max and its presumed wild-type ancestor glycine soja in connection with the origin of cultivated soybean].	nucleotide sequences of cdnas encoding soybean glycinin b4 polypeptide were compared in three soybean cultivars and two plant introductions of wild soybean glycine soja. only two nucleotide substitutions were found in three cultivars g. max, as compared with g. max and g. soja having nucleotide sequences which contain four nucleotide substitutions. these data serve as additional evidence, at molecular level, indicating the origin of g. max from g. soja. on the other hand, the time period require ...	1989	2625198
plant regeneration of wildglycine species from suspension culture-derived protoplasts.	protoplasts isolated from four-week old cell suspension cultures ofglycine canescens f. j. herm andg. clandestina wendl. were cultured in 8p or modified 8p to a multicellular stage. colonies of 0.5 to 1.0 mm diameter were transferred to solid media for callus growth and regeneration. callus consisted of friable masses with compact green nodular areas. organogenesis of both species occurred primarily from the green nodular areas. shoot buds ofg. clandestina did not mature, but shoots ofg. canesce ...	1989	24232998
characterization of a neuraminidase from corynebacterium aquaticum responsible for th polyagglutination.	th polyagglutinability is characterized by the agglutination of the red blood cells (rbc) by arachis hypogaea, medicago disciformis, vicia cretica but, in contrast to the t phenomenon, not by glycine max (glycine soja). because th transformation of rbc has been obtained in vitro, the mechanism of th polyagglutinability expression has been studied and reproduced experimentally. an enzyme with neuraminidase specificity has been isolated from the culture supernatant of corynebacterium aquaticum, an ...	1989	2617954
[lectin-binding properties of the neuroepithelium of the vomeronasal organ, olfactory epithelium proper and the septal organ of masera in mice (semithin section study)].	the apical border of the vomeronasal neuroepithelium, the olfactory epithelium proper, and the septal organ possess varying lectin-binding properties. this can be judged by their ability to bind a peculiar lectin and/or by their reactivity to the given lectin. the following lectins have been used: triticum vulgaris agglutinin (wga), ulex europeus agglutinin (uea-1), arachis hypogea agglutinin (pna), lymbus polyphenus agglutinin (lpa), glycine soja agglutinin (sba) and dolchos diflerus agglutinin ...	1989	2604560
exposure of cryptantigens on erythrocytes in patients with breast cancer.	one hundred thirty-two patients with breast cancer were examined for exposure of cryptantigens on their erythrocytes (rbc) using a lectin panel consisting of arachis hypogaea and glycine soja. eight had exposed cryptantigens; of the eight, five were classified with additional lectins as t-polyagglutination type and three as th-polyagglutination. a control group of 300 healthy blood donors had no exposed cryptantigens on their rbc. these findings could not be correlated with the staging of the tu ...	1988	3365668
electrophoretic study of alpha-d-galactosidases from seeds of glycine soja and vigna radiata possessing erythroagglutinating activity.	polyacrylamide gel electrophoresis in an acidic buffer system was used to study the electrophoretic behaviour of two forms of alpha-d-galactosidase from seeds of soy bean (glycine soja) and mung bean (vigna radiata). the interaction of the enzymes with saccharides was monitored by affinity electrophoresis; for the preparation of affinity gels, water-soluble o-glycosyl polyacrylamide copolymers and polysaccharides were used. alpha-d-galactosidases from both sources interact with immobilized alpha ...	1988	2836454
haemoglobin m-hyde park associated with polyagglutinable red blood cells in a south african family.	twelve of 35 members tested in a large ethnically-mixed south african family were found to have both haemoglobin m type hyde park and persistent polyagglutinable red blood cells. the characteristics of the polyagglutination have not been recorded previously. the cells of affected family members were not agglutinated by arachis hypogea, dolichos biflorus or salvia sclarea, but were agglutinated weakly by salvia horminum and bsii (gsii) and reacted strongly with glycine soja and sophora japonica l ...	1988	3377987
influence of glycine spp. on competitiveness of bradyrhizobium japonicum and rhizobium fredii.	the displacement of indigenous bradyrhizobium japonicum in soybean nodules with more effective strains offers the possibility of enhanced n(2) fixation in soybean (glycine max (l.) merr.). our objective was to determine whether the wild soybean (g. soja sieb. & zucc.) genotype pi 468397 would cause reduced competitiveness of important indigenous b. japonicum strains usda 31, 76, and 123 and thereby permit nodulation by rhizobium fredii, the fast-growing microsymbiont of soybean. in an initial ex ...	1988	16347589
the genomic relationship between glycine max (l.) merr. and g. soja sieb. and zucc. as revealed by pachytene chromosome analysis.	this study was conducted with the objective of determining the genomic relationship between cultivated soybean (glycine max) and wild soybean (g. soja) of the subgenus soja, genus glycine. observations on cross-ability rate, hybrid viability, meiotic chromosome pairing, and pollen fertility in f 1 hybrids of g. max × g. soja and reciprocals elucidated that both species hybridized readily and set mature putative hybrid pods, generated vigorous f1 plants, had a majority of sporocytes that showed 1 ...	1988	24232348
tx polyagglutination in three members of one family.	a case of acute haemolytic anaemia is described in a child. tx polyagglutination of his red cells was observed, but no direct association with the anaemia could be proved. polyagglutination was suspected because of irregularities in the ab0 blood grouping. confirmation of the cryptantigen tx was made when the patient's red cells were tested with lectins including arachis hypogaea, glycine soja, and vicia cretica. examination of family members showed tx polyagglutination on the red cells of 2 sib ...	1987	3116808
two indirect methods for detecting ureide synthesis by nodulated legumes.	two methods were developed for the detection of altered ureide metabolism in legume nodules. both techniques are based on the positive correlation between the presence of high xanthine dehydrogenase (ec 1.2.1.37) specific activity in nodules and the ability of those nodules to produce the ureides, allantoin and allantoic acid. in the first method, nodulated legumes are treated for 2 weeks with a soil drench of allopurinol. after allopurinol treatment, leaves of n(2)-fed, ureide-producing legumes ...	1986	16664857
uneventful administration of plasma products in a recipient with t-activated red cells.	a patient with t-polyagglutinable red cells and a severe coagulopathy provided an opportunity to observe the results of plasma transfusion in the face of t-activation. the patient was a 52-year-old navajo indian with a perforated gall bladder and related sepsis due to clostridium perfringens. the gall bladder was removed surgically. postoperatively, he had severe thrombocytopenia, and prolonged partial thromboplastin and prothrombin times. the patient's red cells were agglutinated by arachis hyp ...	1986	2869600
screening of glycine max and glycine soja genotypes for multiple shoot formation at the cotyledonary node.	to identify genotypes which may give better plant regeneration responses in vitro, multiple shoots were induced from 155 glycine max and 13 glycine soja genotypes from maturity groups '000' to 'vii' on b5 medium supplemented with 1 or 5 μmol benzylaminopurine (bap). the average number of shoots formed show genotype specific and hormone concentration specific responses, with number of shoots ranging from 1 to 12 for different genotypes. the results were reproducible with different seed lots of th ...	1986	24247952
production, characterization, and applications of monoclonal antibodies reactive with soybean nodule xanthine dehydrogenase.	seven monoclonal antibodies were produced against soybean nodule xanthine dehydrogenase, an enzyme involved in ureide synthesis. specificity of the seven monoclonal antibodies for xanthine dehydrogenase was demonstrated by immunopurifying the enzyme to homogeneity from a crude nodule extract using antibodies immobilized to sepharose 4b beads. each monoclonal antibody was covalently bound to sepharose 4b beads for the preparation of immunoaffinity columns for each antibody. all seven antibodies w ...	1986	16664749
discrepancies among published amino acid sequences of soybean leghemoglobins: experimental evidence against cultivar differences as the sources of the discrepancies.	despite discrepancies among charged amino acid residues in published amino acid sequences, isoelectric focusing experiments failed to detect varietal differences in soybean leghemoglobins a, c1, c2, or c3. leghemoglobins from 69 domesticated soybean (glycine max) cultivars and plant introductions and 18 wild soybean (glycine soja) plant introductions were compared; the sources included soybean cultivars used by research groups in obtaining amino acid sequences and most of the ancestors of north ...	1985	4083896
evidence for plasmid- and chromosome-borne multiple nif genes in rhizobium fredii.	rhizobium fredii is a fast-growing rhizobium isolated from the primitive chinese soybean cultivar peking and from the wild soybean glycine soja. this rhizobium harbors nif genes on 150- to 200-megadalton plasmids. by passage on acridine orange plates, we obtained a mutant of r. fredii usda 206 cured of the 197-megadalton plasmid (usda 206c) which carries both nif and nod genes. this strain, however, has retained its symbiotic effectiveness. probing ecori digests of wild-type and cured plasmid dn ...	1985	2992376
genotypic variability of soybean response to agrobacterium strains harboring the ti or ri plasmids.	twenty four diverse cultivars of soybean (glycine max [l.] merrill) and three lines of its annual wild progenitor glycine soja sieb and zucc. were tested for their response to agrobacterium strains harboring either the ti (tumor-inducing) plasmid (pti) from agrobacterium tumefaciens or the ri (root-inducing) plasmid (pri) from agrobacterium rhizogenes following uniform wounding and inoculation. based upon gall weight at 8 weeks postinfection, three g. max cultivars (biloxi, jupiter, and peking) ...	1985	16664035
isolation and expression of rhizobium japonicum cloned dna encoding an early soybean nodulation function.	a first visible step in the nodulation of legumes by rhizobium spp. is the deformation and curling of root hairs. we have identified and cloned dna sequences encoding this function from two strains of rhizobium japonicum (usda 122 and usda 110) with a weakly homologous probe from rhizobium meliloti. root hair curling encoded by the cloned dna fragments was examined on soybeans (glycine soja ) after conjugative transfer of these sequences in broad-host-range vectors to various bacterial genera. p ...	1984	6327649
mode of infection, nodulation specificity, and indigenous plasmids of 11 fast-growing rhizobium japonicum strains.	eleven fast-growing strains of rhizobium japonicum were characterized with respect to indigenous plasmids and abilities to infect (inf+) and nodulate (nod+) cowpea, siratro, wild soybean, and three commercial cultivars of soybean. all strains caused infection via infection threads in root hairs and consistently nodulated cowpea, siratro, and wild soybean in growth pouches. interactions with commercial cultivars of soybean were strikingly strain specific. some combinations were nod-, and infectio ...	1984	6542099
fast-growing rhizobium japonicum that effectively nodulates several commercial glycine max l. merrill cultivars.	several isolates of fast-growing rhizobium japonicum that nodulate the wild soybean glycine soja have been recently described (keyser et al., science 215:1631-1632, 1982). we demonstrate that one of these isolates, designated prc 440 or usda 191, has a wider host range than that previously reported and is able to nodulate several commercial glycine max cultivars as effectively as does slow-growing r. japonicum 61a76. electron microscopic examination revealed no obvious differences between strain ...	1984	16346594
structure and chromosomal arrangement of leghemoglobin genes in kidney bean suggest divergence in soybean leghemoglobin gene loci following tetraploidization.	we have determined the structure of one of the leghemoglobin (lb) genes of phaseolus vulgaris (kidney bean) and deduced the chromosomal arrangement of leghemoglobin genes by genomic hybridizations with lb and two other sequences, each specific to the 5' or 3' region of the soybean leghemoglobin loci. by comparing this organization with two other species of legumes, glycine max (soybean) and g. soja (wild soybean), a phylogeny of leghemoglobin gene loci was traced. the intragenic structure of the ...	1984	16453577
isolation, purification, and partial characterization of formate dehydrogenase from soybean seed.	soybean (glycine soja var beeson) formate dehydrogenase has been isolated, purified, and partially characterized by affinity chromatography. the enzyme is a dimer having a total molecular weight of 100,000 and a subunit weight of 47,000. it has activity over a broad ph range, is stable for months at 4 degrees c, and has k(m) values of 0.6 millimolar and 5.7 micromolar for formate and nad, respectively.	1983	16663314
histochemical studies on lectin binding in reactive lymphoid tissues.	using the avidin-biotin-labeled peroxidase complex (abc) method, the staining reaction of a panel of 12 biotin-labeled lectins was studied in formalin-fixed, paraffin-embedded reactive lymph nodes and tonsils. varying degrees of lectin binding were observed in lymphoid cells and macrophage-histiocytes with concanavalin ensiformis (con a), lens culinaris (lca), phaseolus vulgaris (pha), pisum sativum (psa), ricinus communis (rca), and triticum vulgaris (wga) agglutinins, but no evidence of bindin ...	1983	6827084
organization and expression of leghaemoglobin genes.	leghaemoglobin genes in soybean (glycine max) are present as a moderately reiterated family of sequences. since there are identical restriction site patterns of these sequences in dna isolated from leaf, root, or nodule tissue, the data suggest that no major changes in the organization or methylation of leghaemoglobin genes occur during their induction. cloned soybean leghaemoglobin-cdna cross hybridized with rna from root nodules of kidney bean (phaseolus vulgaris), and to a lesser extent, of p ...	1982	6282418
fast-growing rhizobia isolated from root nodules of soybean.	fast-growing rhizobia have been isolated from soybean root nodules collected in china. these new isolates are physiologically distinct from slow-growing soybean rhizobia. they formed effective nitrogen-fixing associations with wild soybean and an unbred soybean cultivar from china, but were largely ineffective as nitrogen-fixing symbionts with common commercial cultivars of soybeans.	1982	17788491
studies on lectins. li. the role of mn2+ in the activity of the soybean lectin.	in contradistinction to previous reports, the lectin of soybeans (glycine soja) has been shown to retain its erythroagglutinating activity after complete removal of manganese from its molecule. the applied demetallization procedures (dialysis against 0.1 m hcl or 1m acetic acid and against 0.1 m edta following by dialysis against 1 m acetic acid) had no effect on the stability of the lectin subunit structure, as proved by ultracentrifugation analysis or thin-layer chromatography on sephadex g-20 ...	1981	7197555
intestinal absorption of d-galactose and l-leucine and intestinal disaccharidase activities in growing chickens fed different raw legume diets.	a significant (p less than .01) impairment in the rate of growth, along with a significant (p less than .01) inhibition in the rate of in vivo intestinal absorption of d-galactose and l-leucine, and in the in vitro intestinal absorption of d-galactose, was found in growing chickens fed ad libitum over a 60-day period, diets containing the raw legumes vicia faba, glycine soja, vicia ervilia, and phaseolus vulgaris as the main source of protein. furthermore, a significant (p less than .01) reducti ...	1981	7301750
effect of raw legume diets on intestinal absorption of d-galactose by chick.	the effect of four raw legume diets on the intestinal absorption of d-galactose and oxygen consumption were studied in chick. field beans (vicia faba), soybeans (glycine soja), bitter vetch (vicia ervilia), and navy beans (phaseolus vulgaris), were used. the intestinal absorption was determined by both in vivo and in vitro techniques. in vivo, only navy beans and soybeans inhibit intestinal transport of d-galactose, while in vitro all the diets do. oxygen consumption by intestinal rings increase ...	1980	7394275
effect of raw legume diets on disaccharidase activity in the small intestine of chicks.	the effect of four raw legume diets: field beans (vicia faba) (rfb), navy beans (phaseolus vulgaris) (rnb), soybeans (glycine soja) (rsb) and bitter vetch (vicia ervilia) (rbv), on disaccharidase activities in chick small intestine have been studied. maltase and sucrase activities, which vary with age, were determined in 1 to 60 day old animals, rfb and rbv diets had no effect on maltase activity and only increased sucrase activity in 60 day old chicks. both maltase and sucrase activities decrea ...	1980	7192009
host recognition in the rhizobium-soybean symbiosis.	polar binding of rhizobium japonicum to roots and root hairs of glycine soja (l.) sieb. and zucc. is specifically inhibited by d-galactose and n-acetyl-d-galactosamine, haptens of glycine max seed lectin. a protein, immunologically cross-reactive with the g. max seed lectin, is present in g. soja seed extracts. peptide mapping of the purified g. max and g. soja lectins indicates that the two are similar in structure. soybean lectin can be localized on the surface of both g. max and g. soja roots ...	1980	16661488
studies on lectins. xlix. the use of glycosyl derivatives of dextran t-500 for affinity electrophoresis of lectins.	p-aminophenyl glycosides and glycosylamines were coupled to periodate oxidized dextran t-500 either directly or through an epsilon-aminocaproic acid spacer. the new glycosylated derivatives of dextran specifically precipitate lectins having the appropriate carbohydrate specificity, and thus were used in the preparation of affinity gels for affinity electrophoresis of lectins. the apparent strength of interaction of several lectins with carbohydrate residues immobilized in this way was less than ...	1980	6161150
studies on lectins. xliv. the ph dependence of lectin interactions with sugars as determined by affinity electrophoresis.	the ph dependence of association constants of the lectin-sugar complexes was determined by means of affinity electrophoresis. all the lectins studied (from the seeds of dolichos biflorus, glycine soja, lens esculenta and vicia cracca and of the fruiting body of marasmius oreades) were characterized by a similar course of ph dependence of the association constants, with the maximum values at ph 7--9. for concanavalin a and the l-fucose binding ulex europaeus lectin only the association constants ...	1979	33723
studies on lectins. xxxvi. properties of some lectins prepared by affinity chromatography on o-glycosyl polyacrylamide gels.	a number of lectins has been purified by affinity chromatography on o-glycosyl polyacrylamide gels. the lectins isolated (and the particular sugar ligands used in the affinity carriers) are as follows: anguilla anguilla, serum (alpha-l-fucosyl-), vicia cracca, seeds; phaseolus lunatus, seeds; glycine soja, seeds; dolichos biflorus, seeds; maclura pomifera, seeds; sarothamnus scoparius, seeds; helix pomatia, ablumin glands; clitocybe nebularis, fruiting bodies (all n-acetyl-alpha-d-galactosaminyl ...	1978	563738
additional precipitation reactions of lectins with human serum glycoproteins.	highly purified human serum glycoproteins were treated with neuraminidase and examined for their cross reaction with several lectins with anti-galactosyl specificity: beta-d-galactosyl structures are thought to be the main terminal sugar residues that become attached de novo after removal of neuraminic acid. the following lectins were tested: tridacnin from the bivalve clams tridacna maxima and tridacna gigas, the agglutinin from the sponge axinella polypoides, the lectin from the roach rutilus ...	1978	564939
studies on lectins. xl. o-glycosyl derivatives of spheron in affinity chromatography of lectins.	free monosaccharides can be used for direct glycosylation of spheron, a spherical macroporous hydroxyalkyl methacrylate-ethylene dimethacrylate copolymer, in a reaction that proceeds at room temperature in dioxane medium under catalysis of dry hcl or bf3. derivatives of l-fucose, d-galactose, d-glucose, d-mannose, n-acetyl-d-galactosamine and n-acetyl-d-glucosamine thus prepared from spheron beads have been shown to be efficient affinity carriers in isolation of lectins from seeds of canavalia e ...	1978	638203
[examination and composition of some legume seeds (author's transl)].	the chemical composition of some legume seeds is reviewed. it was found that soy (glycine soja), field beans (vicia faba), vicia sativa and lens culingris (red seeds) have the highest protein content. there were only minor differences in amino acid composition except for glutamic acid. the lysine content of legume seeds was high. lysine is the most limiting essential amino acid in cereal proteins, therefore cereal products can be improved nutritionally by addition of legume seed flour. examinati ...	1977	560092
lipids in plant tissue cultures. v. effect of environmental conditions on the lipids of glycine soja and brassica napus cultures.	the environmental conditions prevailing during growth of plant tissue cultures affect the concentration of certain lipid classes and the fatty acid patterns of the total lipids. irrespective of whether the cultures are grown under continuous illumination or in the dark, aerated cultures contain larger proportions of sterols, steryl esters, steryl glycosides and various sterylglycolipids and lower concentrations of squalene than non-aerated cultures. the lipids of the latter contain larger propor ...	1975	1168102
physical and chemical studies of a low-molecular-weight form of urease.	1. a new form of enzymically active jack-bean [canavalia ensiformis (l.) dc] urease corresponding to an s(20,w) value of 11.8s and a molecular weight of 260000 was investigated. 2. conversion of 18s urease (ec 3.5.1.5) into the 12s form depends on both low protein concentration and ph. above ph5.3 urease exists in the 18s form and below ph4.8 in the 12s form; between these two ph values a 12s-18s rapid-equilibrium process is observed. 3. comparison of the properties of 18s and 12s urease indicat ...	1968	5691753
blood sugar and total cholesterol lowering effect of glycine soja (sieb and zucc.), mucuna pruriens (d.c.) and dolichos biflorus (linn.) seed diets in normal fasting albino rats.		1968	5732448
specificity of glycine soja agglutinins.		1963	14103469
influence of enzyme and substrate concentrations on the decomposition of urea by commercial urease and urease obtained from glycine soja beans in phosphate buffer.		1961	13685889
studies on the influence of stannous chloride on the decomposition of urea by soyabean (glycine soja) urease.		1960	13795787
haemagglutinins of glycine soja.		1955	13272759
crossing experiments with glycine soja and g. gracilis.		1952	12989433
differential expression of a wrky gene between wild and cultivated soybeans correlates to seed size.	soybean (glycine max) probably originated from the wild soybean (glycine soja). glycine max has a significantly larger seed size, but the underlying genomic changes are largely unknown. candidate regulatory genes were preliminarily proposed by data co-localizing rna sequencing with the quantitative loci (qtls) for seed size. the soybean gene locus soywrky15a and its orthologous genes from g. max (gmwrky15a) and g. soja (gswrky15a) were analyzed in detail. the coding sequences were nearly identic ...	0	28472462