Publications

trypsin inhibitor iii from squash seeds (cucurbita maxima), its reactive site and proposed amino acid sequence.	the amino acid sequence of trypsin inhibitor iii isolated from squash seeds (cucurbita maxima) was established by edman degradation and standard enzymatic and chemical techniques. the inhibitor consists of 28 amino acids crosslinked by two disulfide bridges. arginine in position 5 was shown to be present at the reactive site of the inhibitor.	1981	7275008
calcium-binding protein in sieve tube exudate.	a calcium-binding macromolecule, with an estimated molecular weight greater than 100,000, was detected in phloem exudate from cucurbita maxima and related species. the macromolecule was a component of sieve tube sap, rather than a contaminant leached from cell walls or cut parenchyma cells during exudate collection. the protein nature of this macromolecule was deduced from its size, lability, susceptibility to proteolytic digestion, and by the dependence of calcium-binding activity on thiol-prot ...	1981	24302204
auxin-stimulated atpase in membrane fractions from pumpkin hypocotyls (cucurbita maxima l.).	membrane fractions from cucurbita maxima hypocotyls were isolated in a medium which inhibits the action of endogenous phospholipases. after removal of soluble phosphatases by sepharose 2b-cl column chromatography, an auxin-stimulated atpase activity was found in membrane fractions from linear sucrose gradients. in the presence of 10(-4) m phenylacetic acid (paa), the stimulation by indol-3-acetic acid (iaa) exhibited a bimodal concentration dependence with maximal stimulation of about 50% at 10( ...	1981	24302108
isolation and partial amino acid sequence of the trypsin inhibitor from the seeds of cucurbita maxima.	trypsin inhibitor from squash (cucurbita maxima) seed was extracted with 0.1 m-acetate buffer, ph 4.5, purified on immobilized trypsin, and separated by se-sephadex c-50 chromatography into three active fractions. all of them inhibited trypsin to the same extent, showed no antichymotrypsin or antikallikrein activity, had a similar molecular weight (about 3300), and contained no tryptophan, phenylalanine or threonine. the partial amino acid sequence of tryptic and peptic peptides of fraction iii ...	1980	6791422
calcium distribution in globoid crystals of cucurbita cotyledon protein bodies.	energy-dispersive x-ray analysis was used to investigate the location of globoid crystals with relatively high ca levels within cotyledons of cucurbita maxima, cucurbita mixta, and cucurbita andreana. the small globoid crystals in both upper and lower epidermal cells commonly contained ca. ca was present in globoid crystals of all provascular regions with the exception of the very small provascular regions of c. maxima. in c. maxima and c. mixta cotyledons, some cases were observed where ca was ...	1979	16660825
energy-dispersive x-ray analysis of phosphorus, potassium, magnesium, and calcium in globoid crystals in protein bodies from different regions of cucurbita maxima embryos.	the seeds of cucurbita maxima contain protein bodies with electrondense globoid crystals. because of their density globoid crystals are ideal material for energy-dispersive x-ray (edx) analysis studies of elemental composition. fixation trials were carried out to test globoid crystal extraction during glutaraldehyde fixation, water washing, and ethanol dehydration. glutaraldehyde fixation without subsequent washing or dehydration alone produced no significant changes in elemental composition of ...	1978	16660439
peroxidases do not move in phloem.	phloem exudates from grafts between queensland blue pumpkin (cucurbita maxima duch.) and candy red hawkesbury watermelon (citrullus vulgaris schrad.) were analysed by iso-electric focusing to detect iso-enzymes of peroxidase. these enzymes did not move in intact phloem but, when stems were cut, they surged rapidly through graft unions.	1978	24413951
the isolation and some properties of a lectin (haemagglutinin) from cucurbita phloem exudate.	the occurrence of high haemagglutinating (lectin) activity in phloem exudate from three cucurbit species is reported. the protein responsible for this lectin activity in cucurbita maxima duch. has been isolated by cation exchange chromatography on sepharose and identified by gel electrophoresis. the lectin showed agglutinating activity at concentrations as low as 0.1 μg/ml. no sugar, including those transported in the phloem of these species, interacted with agglutination. the lectin could not b ...	1978	24408004
correlation between oxygen availability, energy charge, and protein synthesis in squash cotyledons isolated from germinating seeds.	the influence of o(2) availability on the rate of protein synthesis, the levels of rna and of adenylates, and the value of the energy charge in squash (cucurbita maxima) cotyledons isolated from seeds germinated for 15 or 28 hours at different o(2) concentration (3% or 20% o(2)) has been investigated.the rate of protein synthesis is five times lower in cotyledons maintained in 3% o(2) than in those maintained in 20% o(2). also net rna synthesis is almost blocked in 3% o(2), while in 20% o(2) it ...	1978	16660244
a re-examination of the reputed control of cotyledonary metabolism by the axis.	a re-examination has been made of the supposed regulation by the axis of cotyledonary isocitrate lyase (ec 4.1.3.1) activity in cucumis sativas l. and cucurbita maxima duch. and greening in cucumis. when precautions are taken to ensure that isolated cotyledons and cotyledons attached to the axis have equal access to oxygen and/or water no axis effects can be observed. the results underline the importance of maintaining excised plant parts in comparable physical conditions if meaningful physiolog ...	1976	24425000
synthesis of p-protein in mature phloem of cucurbita maxima.	cotyledons of cucurbita maxima duch. seedlings were provided with (14)c-labeled amino acids for 12 h. besides the bulk of labeled amino acids the sieve-tube exudate also carried labeled proteins. 80% of the incorporated radioactivity was found in the p-protein, 20% in a neutral protein, and traces were found in acidic proteins after fractionation on diethyl-aminoethyl cellulose columns. the radioactive elutes were characterized by autoradiographs of both disc- and sodium dodecyl sulfate-gelelect ...	1976	24424987
the heterogeneity of phloem exudate proteins from different plants: a comparative survey of ten plants using polyacrylamide gel electrophoresis.	proteins in sieve tube exudate from ricinus communis l., acer pseudoplatanus l., aesculus hippocastanum l., cucumis melo l., and two cultivars each of cucumis sativus l., cucurbita pepo l. and cucurbita maxima duchesne were fractionated and compared using polyacrylamide gel electrophoresis. striking differences in major exudate proteins were displayed among the genera and species examined. even cultivars within a single species, although showing general similarities, differed in some prominent p ...	1976	24424985
a comparative analysis of phloem exudate proteins from cucumis melo, cucumis sativus and cucurbita maxima by polyacrylamide gel electrophoresis and isoelectric focusing.	proteins in sieve tube exudate from cucumis melo l., cucumis sativus l. and cucurbita maxima duch. were analysed by gel electrophoresis and isoelectric focusing. estimated molecular weights and isoelectric points for the major and minor proteins from each plant species are presented. electrophoresis revealed striking differences between the protein complements of exudatc from the two genera investigated. similarly, although a few exudate proteins from the two species of cucumis possessed identic ...	1976	24424601
changes in the cotyledons of cucurbita maxima during germination. v. the nuclear envelope.		1975	1152111
protein body composition in cuburbita maxima cotyledons as determined by energy dispersive x-ray analysis.	energy dispersive x-ray analysis was used to study the composition of certain protein body components in cucurbita maxima cotyledons. the globoid crystal was rich in phosphorus, potassium, and magnesium. this elemental composition provides further evidence that the globoid crystal in squash cotyledon protein bodies is composed of phytin, a myoinositol hexaphosphoric acid salt of potassium and magnesium. calcium, a common component of phytin in many species, was absent or present in only trace am ...	1975	16659190
filament formation in vitro of a sieve tube protein from cucurbita maxima and cucurbita pepo.	phloem proteins of the sieve tube exudate from cucurbita maxima duch. and cucurbita pepo l. were investigated as to their filament forming ability in vitro. from the two main proteins (116000 dalton, 30000 dalton) only the 116000 dalton protein was found to form reversibly distinct filaments of 6-7 nm diameter upon removal of sh-protecting agents from the buffer, whereas the 30000 dalton protein was precipitated as amorphous material under these conditions. the protein filaments were similar to ...	1975	24430373
structure and biochemistry of phloem-proteins isolated from cucurbita maxima.		1974	4210435
sieve-tube proteins from cucurbita maxima.	the two main proteins from the phloem exudate of cucurbita maxima duchesne have been isolated by ammonium-sulfate precipitation, deae-cellulose chromatography, and gel filtration, and have been characterized. they comprise about 40% each of the total protein. the amino-acid composition of these two proteins has been determined. both are highly basic with an iep above 9.5. the smaller protein has a molecular weight of ca. 30000 as determined by analytical ultracentrifugation, gel filtration, and ...	1974	24442451
the biosynthesis of a c19-gibberellin from mevalonic acid in a cell-free system from a higher plant.	incubation of a cell-free system from immature seeds of cucurbita maxima duch. with [(14)c]ga12-aldehyde derived from [(14)c]mevalonic acid in the same system yielded the c19-gibberellin, [(14)c]ga4, in addition to the c20-gibberellins, [(14)c]ga37, [(14)c]ga13 and [(14)c]ga43. (ga-gibberellin).	1974	24442706
adenosine triphosphatase in the phloem of cucurbita.	the distribution of adenosine triphosphatase (atpase) activity in the phloem of petioles and minor veins of cucurbita maxima has been studied using a lead phosphate precipitation procedure. atpase activity was localized in sieve elements, companion cells and parenchyma cells. activity was found at the cell surfaces, associated with the dispersed p-protein of mature sieve elements, in mitochondria, sieve-element reticulum, and at specific regions of the cell walls. it is suggested that the atpase ...	1973	24474397
p-protein distribution in mature sieve elements of cucurbita maxima.	portions of the hypocotyls of 16-day-old cucurbita maxima plants, from which the cotyledons and first foliage leaves had been removed 2 days earlier, were fixed in glutaraldehyde and postfixed in osmium tetroxide for electron microscopy. in well over 90% of the mature sieve elements examined the p-protein was entirely parietal in distribution in both the lumina and sieve-plate pores. in addition to the parietal p-protein, the unoccluded sieve-plate pores were lined by narrow callose cylinders an ...	1972	24474154
the amino acid sequence of cytochrome c from cucurbita maxima l. (pumpkin).	the amino acid sequence of pumpkin cytochrome c was determined on 2mumol of protein. some evidence was found for the occurrence of two forms of cytochrome c, whose sequences differed in three positions. pumpkin cytochrome c consists of 111 residues and is homologous with mitochondrial cytochromes c from other plants. experimental details are given in a supplementary paper that has been deposited as supplementary publication sup 50005 at the national lending library for science and technology, bo ...	1971	5131733
proteins of the sieve-tube exudate of cucurbita maxima.	sieve-tube exudate which appears on cut surfaces of stems of cucurbita maxima as distinct droplets has been depicted in electron micrographs of longitudinal sections of the phloem. the exudate, which was produced from mature sieve tubes only, contained filaments of p-protein, but no mitochondria or vesicles of endoplasmic reticulum. the water-soluble part of the exudate contained at least 12 proteins, as shown by disc-electrophoresis. enzymic activity was found for peroxidases, acid phosphatases ...	1971	24488194
molecular weights of cucurbita sieve tube proteins.	by means of sds-polyacrylamide-gel electrophoresis, molecular weights of 15000, 28000, 59000, 116000 and 220000 were determined for the main sieve tube proteins from cucurbita maxima.	1971	24487555
artificial tetraploids of squash (cucurbita maxima duch.).		1971	5163382
[spectrophotometric analysis of phytochrome in the embryo of cucurbita pepo and cucurbita maxima].	phytochrome detection and △ (△o.d.) measurements were made for the first time in a single embryo of pumpkin or gourd thanks to an apparatus adjusted to the differential spectrophotometer constructed by dr. spruit (wageningen). observed △ (△o.d.) values are comparable with usual values obtained by analysis of several seeds in a cuvette. the differential spectrum obtained has the same characteristics as the spectra of seedlings and seeds which can be found in the present literature. the time cours ...	1970	24499981
protein filaments-structural components of the phloem exudate : i. observations with cucurbita and nicotiana.	fine structure and chemical composition of the phloem exudate of cucurbita maxima and nicotaiana glauca x suaveolens are investigated. filamentous structures, several microns in length, are identified as structural components of the exudate by means of negative staining and electron microscopy. two types of filaments are described: one form measures nearly 40 å in diameter and shows a beaded appearance with regular spacings of about 50 å; it is termed "elementary filament". the second form has a ...	1970	24497023
[insoluble proteins of gourd seeds (cucurbita maxima duch)].		1970	5429060
[soluble proteins of the seed of gourd (cucurbita maxima duch.)].		1970	5429059
uptake of 3-o-methylglucose by healthy and hypomyces-infected squash hypocotyls.	rates of uptake of 3-o-methylglucose (meg) by squash (cucurbita maxima) hypocotyl sections from above lesions caused by hypomyces solani f. sp. cucurbitae, race 1, are 2-fold greater than uptake by comparable tissues from healthy plants. kinetic analyses indicate (i) that a single (constitutive) carrier system, with a michaelis constant (km) of 25 to 30 mm, mediates the transport of meg into healthy hypocotyl cells and (ii) that an additional (inducible) system with a much lower km (ca. 2 mm) is ...	1969	16657201
effect of infection by hypomyces solani f. sp. cucurbitac on apparent free space, cell membrane permeability, and respiration of squash hypocotyls.	initial symptoms and increases in respiration, apparent free space, and rate of leakage of amino acids occurred concomitantly in squash (cucurbita maxima dcne) hypocotyls infected by hypomyces solani f. sp. cucurbitae snyd. and hans. young, rapidly expanding lesions had greater respiratory rates and apparent free space than comparable tissues from healthy plants.hypocotyl tissues above (1-45 mm) lesions possessed greater endogenous respiratory rates (2-3 times) and lower respiratory quotients th ...	1968	16656953
cucurbitacin b delta 23-reductase from cucurbita maxima. ii. cofactor requirements, enzyme kinetics, substrate specificity and other characteristics.		1968	4384331
p protein in the phloem of cucurbita. ii. the p protein of mature sieve elements.	during maturation of sieve elements in cucurbita maxima duchesne, the p-protein bodies (slime bodies) usually disperse in the tonoplast-free cell. in some sieve elements the p-protein bodies fail to disperse. the occurrence of dispersal or nondispersal of p-protein bodies can be related to the position of the sieve elements in the stem or petiole. in the sieve elements within the vascular bundle the bodies normally disperse; in the extrafascicular sieve elements the bodies often fail to disperse ...	1968	5664205
fractionation of fatty acids of cucurbita maxima seed oil with urea.		1968	5641684
cucurbitacin b delta 23-reductase from cucurbita maxima. i. assay methods, isolation and purification.		1968	5640163
p protein in the phloem of cucurbita. i. the development of p-protein bodies.	light and electron microscopical observations of the cells of the phloem of cucurbita maxima have shown that two distinct types of p-protein bodies are formed: a larger type which arises as fine fibrils and a smaller type which apparently arises as groups of tubules. the tubules of the smaller type of body measure 242 +/- 3.6 (se) a (n = 48) and appear morphologically identical with the p1-protein tubules of nicotiana tabacum l. in some of these p1-protein bodies the tubules are arranged in a re ...	1968	5675415
anthelmintic activity of cucurbita maxima (kaddu) seeds.		1967	6076370
[on the soluble proteins in the water from seeds of cucurbita maxima duch].		1966	4225958
effect of blanching and frozen storage on ascorbic acid in baby blue winter squash.		1964	14133711
a mottled-leaf character in winter squash; inherited as a dominant mendelian character.		1946	21015950
bacterial leafspot on hubbard squash.		1926	17735959
crystal structure of korean pine (pinus koraiensis) 7s seed storage protein with copper ligands.	the prevalence of food allergy has increased in recent years, and korean pine vicilin is a potential food allergen. we have previously reported the crystallization of korean pine vicilin purified from raw pine nut. here we report the isolation of vicilin mrna and the crystal structure of korean pine vicilin at 2.40 å resolution. the overall structure of pine nut vicilin is similar to the structures of other 7s seed storage proteins and consists of an n-terminal domain and a c-terminal domain. ea ...	0	24328105