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extremely low doses of arsenic affect in vitro pollen germination.this study deals with the effects of extremely low doses or high dilutions of pharmacological compounds on in vitro pollen germination of kiwifruit (actinidia deliciosa).201324030446
tapetum and middle layer control male fertility in actinidia deliciosa.dioecism characterizes many crop species of economic value, including kiwifruit (actinidia deliciosa). kiwifruit male sterility occurs at the microspore stage. the cell walls of the microspores and the pollen of the male-sterile and male-fertile flowers, respectively, differ in glucose and galactose levels. in numerous plants, pollen formation involves normal functioning and degeneration timing of the tapetum, with calcium and carbohydrates provided by the tapetum essential for male fertility. t ...201323965617
the prevalence of pfs and prevalence and characteristics of reported food allergy; a survey of uk adults aged 18-75 incorporating a validated pfs diagnostic questionnaire.pollen-food syndrome (pfs), a food allergy affecting pollen-sensitized individuals, is likely to be the most prevalent food allergy in adults, estimated to affect 50-90% of people allergic to birch tree pollen.201323889246
mating behavior of cnephasia jactatana (lepidoptera: tortricidae), an important pest of kiwifruit.understanding mating behavioral sequence helps us identify the mechanisms of mate assessment and choice, and better evaluate behavior-based pest control strategies. here we describe the mating behavior of cnephasia jactatana walker whose females release a sex pheromone, and determine the effect of male mating status on reproductive success. the mating sequence starts when males approach females and display courtship behavior with antennation and fanning wings. both males and females may end a ma ...201323865204
over-accumulation of putrescine induced by cyclohexylamine interferes with chromium accumulation and partially restores pollen tube growth in actinidia deliciosa.both trivalent and hexavalent chromium, i.e., cr(iii) and cr(vi), respectively, were previously demonstrated to affect in vitro germination and ultrastructure of kiwifruit (actinidia deliciosa) pollen. in the present work, the response to chromium in germinating pollen was evaluated in terms of changes in the polyamine profile. slight, though significant, increases in free spermidine and spermine occurred after exposure to cr(iii), while the levels remained almost unchanged after cr(vi) treatmen ...201323835360
conformational mobility of active and e-64-inhibited actinidin.actinidin, a protease from kiwifruit, belongs to the c1 family of cysteine proteases. cysteine proteases were found to be involved in many disease states and are valid therapeutic targets. actinidin has a wide ph activity range and wide substrate specificity, which makes it a good model system for studying enzyme-substrate interactions.201323803410
light and abiotic stresses regulate the expression of gdp-l-galactose phosphorylase and levels of ascorbic acid in two kiwifruit genotypes via light-responsive and stress-inducible cis-elements in their promoters.ascorbic acid (asa) plays an essential role in plants by protecting cells against oxidative damage. gdp-l-galactose phosphorylase (ggp) is the first committed gene for asa synthesis. our research examined asa levels, regulation of ggp gene expression, and how these are related to abiotic stresses in two species of actinidia (kiwifruit). when leaves were subjected to continuous darkness or light, aba or meja, heat, or a hypoxic environment, we found some correlation between the relative levels of ...201323775440
effects of kivia powder on gut health in patients with occasional constipation: a randomized, double-blind, placebo-controlled study.to evaluate the efficacy of kivia powder on supporting overall gut health through the relief of the discomfort of occasional constipation.201323758673
isolation and developmental expression analysis of l-myo-inositol-1-phosphate synthase in four actinidia species.myo-inositol (mi) is an important polyol involved in cellular signal transduction, auxin storage, osmotic regulation, and membrane formation. it also serves as a precursor for the production of pinitol, ascorbic acid, and members of the raffinose family. the first committed step for mi formation is catalyzed by l-myo-inositol-1-phosphate synthase (mips). we isolated mips cdna sequences from actinidia eriantha, actinidia rufa, and actinidia arguta and compared them with that of actinidia delicios ...201324184456
a biophysical model of kiwifruit (actinidia deliciosa) berry development.a model of kiwifruit berry development is presented, building on the model of fishman and génard used for peach fruit. that model has been extended to incorporate a number of important features of kiwifruit growth. first, the kiwifruit berry is attached to the stem through a pedicel/receptacle complex which contributes significantly to the hydraulic resistance between the stem and the fruit, and this resistance changes considerably during the season. second, much of the carbohydrate in kiwifruit ...201324123250
dispersive liquid-liquid microextraction for the determination of three cytokinin compounds in fruits and vegetables by liquid chromatography with time-of-flight mass spectrometry.the paper presents a novel approach for the determination of three cytokinin compounds, thidiazuron (tdz), 1,3-diphenylurea (1,3-dpu) and forchlorfenuron (cppu), in fruit and vegetables samples using liquid chromatography with electrospray ionization and time-of-flight mass spectrometry (lc-esi-tofms). analytes were extracted from the sample matrix with ethanol, and the extract, after dilution with water, was submitted to dispersive liquid-liquid microextraction (dllme). once acetonitrile and 1, ...201324148418
changes in the bound aroma profiles of 'hayward' and 'hort16a' kiwifruit (actinidia spp.) during ripening and gc-olfactometry analysis.bound volatiles are recognised as a potential source of aroma compounds in fruits. in this study, the bound volatiles of actinidia deliciosa 'hayward' and a. chinensis 'hort16a' were studied at three different ripening stages. the bound volatile content tended to increase as the fruit ripened from under-ripe to ripe, and then decreased in over-ripe fruit. glycosides of (z)-3-hexen-1-ol and hexanol (green-note volatiles) were present in considerable amounts. β-glucosidase activity in 'hayward' an ...201323199989
identification and thermal stability of purple-fleshed sweet potato anthocyanins in aqueous solutions with various ph values and fruit juices.thirteen anthocyanins were identified in the purple-fleshed sweet potato cultivar jihei no. 1. the main anthocyanins were 3-sophoroside-5-glucoside derivatives from cyanidin and peonidin, acylated with p-hydroxybenzoic acid, ferulic acid, or caffeic acid. a unique anthocyanin, delphinidin-3,5-diglucoside was also found. the thermal stability of purple-fleshed sweet potato anthocyanins (pspas) followed a first-order kinetics model. aqueous solutions with various ph (2, 3, 4, 5, and 6) and fruit j ...201323194545
kiwifruit consumption favourably affects plasma lipids in a randomised controlled trial in hypercholesterolaemic men.the unique composition of green kiwifruit has the potential to benefit cvd risk. the aim of the present study was to investigate the effect of consuming two green kiwifruits daily in conjunction with a healthy diet on plasma lipids and other metabolic markers and to examine response according to apoe genotype in hypercholesterolaemic men. after undergoing a 4-week healthy diet, eighty-five hypercholesterolaemic men (ldl-cholesterol (ldl-c) > 3.0 mmol/l and tag < 3 mmol/l) completed an 8-week ran ...201323151354
kiwifruit allergy across europe: clinical manifestation and ige recognition patterns to kiwifruit allergens.kiwifruit is a common cause of food allergy. symptoms range from mild to anaphylactic reactions.201323141741
lc-ms-ms determination and pharmacokinetic study of the novel anti-tumor candidate drug teoa in rats.2α, 3α, 24-trihydroxyurs-12-en-28-oic acid (teoa) is a prominent ursane-type triterpenoid isolated from the root of actinidia deliciosa, which has numerous pharmacological activities. to date, there is no report on the pharmacokinetic characterization of teoa in biological samples. a specific, sensitive and robust liquid chromatography-tandem mass spectrometry (lc-ms-ms) method is reported for the fast assay of teoa in rat plasma. plasma sample preparation was based on liquid-liquid extraction w ...201323139245
maternal inheritance of mitochondrial genomes and complex inheritance of chloroplast genomes in actinidia lind.: evidences from interspecific crosses.the inheritance pattern of chloroplast and mitochondria is a critical determinant in studying plant phylogenetics, biogeography and hybridization. to better understand chloroplast and mitochondrial inheritance patterns in actinidia (traditionally called kiwifruit), we performed 11 artificial interspecific crosses and studied the ploidy levels, morphology, and sequence polymorphisms of chloroplast dna (cpdna) and mitochondrial dna (mtdna) of parents and progenies. sequence analysis showed that th ...201323337924
mood improvement in young adult males following supplementation with gold kiwifruit, a high-vitamin c food.enhanced intakes of fruit and vegetables have been associated with improved psychological well-being. we investigated the potential mood-enhancing effects of kiwifruit, a fruit rich in vitamin c and a number of other important micronutrients. young adult males (n 35) were supplemented with either half or two kiwifruit/d for 6 weeks. profile of mood states questionnaires were completed at baseline and following the intervention. no effect on overall mood was observed in the half a kiwifruit/d gro ...201325191573
characterisation of kiwifruit and asparagus enzyme extracts, and their activities toward meat proteins.two plant enzyme extracts from kiwifruit and asparagus were evaluated for their ability to hydrolyse commercially available substrates and proteins present in both beef connective tissue and topside myofibrillar extracts. the results show significant differences in protease activity depending on the assay used. protease assays with connective tissue and meat myofibrillar extracts provide a more realistic evaluation of the potential of the enzymes for application in meat tenderization. overall, t ...201323122154
clonal differences in antioxidant activity and bioactive constituents of hardy kiwifruit (actinidia arguta) and its year-to-year variability.hardy kiwifruit (actinidia arguta) is a new species, commercially grown in recent years. total phenolics (tpc), vitamin c (taa) content, antioxidant activity (aa) and their year-to-year variability in seven hardy kiwifruit clones were evaluated. tpc was determined using the folin-ciocalteu reagent assay. taa was estimated by determination of l-ascorbic acid and l-dehydroascorbic acid levels using high-performance liquid chromatography. aa was measured using diphenyl-1-picryl-hydrazyl (dpph), 2,2 ...201323070974
expression patterns and promoter characteristics of the gene encoding actinidia deliciosa l-galactose-1-phosphate phosphatase involved in the response to light and abiotic stresses.the gene encoding l-galactose-1-phosphate phosphatase (gpp) plays a central role in ascorbic acid (asa) biosynthesis in plants. here, we report asa contents, gpp expression, and functioning of its promoter in response to light, exogenous stress-signalling hormones, or abiotic stresses in kiwifruit (actinidia deliciosa). to identify the upstream region of gpp required for promoter activity, we constructed a series of promoter deletion derivatives. each construct was analyzed by agrobacterium-medi ...201323070919
molecular cloning and characterization of a cdna encoding kiwifruit l-myo-inositol-1-phosphate synthase, a key gene of inositol formation.l-myo-inositol-1-phosphate synthase (mips; ec 5.5.1.4) is the key enzyme involved in de novo synthesis of myo-inositol, leading to numerous cellular functions. we isolated an open reading frame of actinidia deliciosa mips (admips), which is 1,533 bp long and codes for 510 amino acids, with a predicted molecular weight of 56.3 kda. sequence analysis revealed its high similarity with mips proteins from other organisms. gene expression and enzyme activity were highest in flower and young fruit. tra ...201323065229
the effects of fruit maturation, delayed storage and ethylene treatment on the incidence of low-temperature breakdown of 'hayward' kiwifruit.low-temperature breakdown (ltb), a disorder inducing quality loss, during and after cold storage of 'hayward' kiwifruit was investigated. harvested kiwifruits during fruit maturation or after delayed storage (ds) at 20 °c for 0, 1, 2, 3 and 4 weeks and 1 µl l⁻¹ ethylene treatment for 24 h were stored at -0.5 °c for 24 weeks and additional ripening at 20 °c for 5 days. fruit quality indices and ltb incidence and severity were determined before and after treatments.201322777890
influence of cultivation parameters on the composition of volatile compounds and physico-chemical characteristics of kiwi fruit.the effect of four cultivation parameters (post-maturity harvest date, storage period at 0 °c and input of nitrogen or potassium fertilisers) on the physico-chemical characteristics and composition of volatile compounds in kiwi fruit (actinidia deliciosa) were evaluated. five physico-chemical parameters were selected, namely, ph, total acidity, dry matter, conductivity and refractive index. to our knowledge, no published data are available concerning the influence of nitrogen or potassium fertil ...201322936621
development of an immunochromatographic assay based on carbon nanoparticles for the determination of the phytoregulator forchlorfenuron.rapid analytical methods enabling the determination of diverse targets are essential in a number of research areas, from clinical diagnostics to feed and food quality and safety. herein, the development of a quantitative immunochromatographic assay for the detection of the synthetic phytoregulator forchlorfenuron (cppu) is described. the competitive lateral flow immunoassay (lfia) was based on the immobilization onto a nitrocellulose membrane of an ovalbumin-cppu conjugate (test line) and on the ...201323202348
headspace gas-liquid chromatographic determination of dithiocarbamate residues in fruits and vegetables with confirmation by conversion to ethylenethiourea.ethylene-bis-dithiocarbamate (ebdc) residues were determined as carbon disulfide (cs2) by an improved headspace gas-liquid chromatographic (glc) procedure. among 837 samples of 30 agricultural commodities tested, 43% contained residues above the detection limit for the method: 100% of broccoli samples; 80% of cabbage, kiwifruit, and grape samples; and 71% of cucumber samples. most of the residues in kiwifruit were on or near the skin. elimination of false-positive detections by the improved meth ...20138946720
in vitro studies of polyphenols, antioxidants and other dietary indices in kiwifruit (actinidia deliciosa).the main aim of the present study was the evaluation of proteins and antioxidant potential in ethylene-treated kiwifruit during the first 10 days of ripening. kiwifruit samples were randomly divided into two groups: treated and untreated. flesh firmness, sensory value, visual score, free sugars, soluble solids, ethylene biosynthesis, proteins, dietary fibers, total polyphenols and antioxidant potential were determined in both groups. ethylene (100 ppm) at 20 degrees c for 24 h was used in the tr ...201316849119
pseudomonas syringae pv. actinidiae from recent outbreaks of kiwifruit bacterial canker belong to different clones that originated in china.a recently emerged plant disease, bacterial canker of kiwifruit (actinidia deliciosa and a. chinensis), is caused by pseudomonas syringae pv. actinidiae (psa). the disease was first reported in china and japan in the 1980s. a severe outbreak of psa began in italy in 2008 and has spread to other european countries. psa was found in both new zealand and chile in 2010. to study the evolution of the pathogen and analyse the transmission of psa between countries, genomes of strains from china and jap ...201323555547
genomic analysis of the kiwifruit pathogen pseudomonas syringae pv. actinidiae provides insight into the origins of an emergent plant disease.the origins of crop diseases are linked to domestication of plants. most crops were domesticated centuries--even millennia--ago, thus limiting opportunity to understand the concomitant emergence of disease. kiwifruit (actinidia spp.) is an exception: domestication began in the 1930s with outbreaks of canker disease caused by p. syringae pv. actinidiae (psa) first recorded in the 1980s. based on snp analyses of two circularized and 34 draft genomes, we show that psa is comprised of distinct clade ...201323935484
using fundamental knowledge of induced resistance to develop control strategies for bacterial canker of kiwifruit caused by pseudomonas syringae pv. actinidiae. 201323437017
characterization of the complete genome of a novel citrivirus infecting actinidia chinensis.a ssrna virus from kiwifruit (actinidia spp.) was identified as a member of the family betaflexiviridae. it was mechanically transmitted to the herbaceous indicators nicotiana benthamiana, n. clevelandii, n. glutinosa and n. occidentalis. the complete genome was comprised of three orfs and a 3'poly (a) tail. phylogenetic analysis of the entire genome indicated it was a novel member of the genus citrivirus (family betaflexiviridae). the complete nucleotide sequence differed from that of citrus le ...201323494225
biochemical and immunological characterization of a recombinantly-produced antifungal cysteine proteinase inhibitor from green kiwifruit (actinidia deliciosa).plant proteinase inhibitors are considered important defense molecules against insect and pathogen attack. the cysteine proteinase inhibitor (cpi) from green kiwifruit (actinidia deliciosa) belongs to the cystatin family and shows potent antifungal activity (in vitro and in vivo). however, the low abundance of this molecule in fruit (6μg/g of fresh fruit) seems to limit further investigations on the interaction between phytocystatin and photopathogenic fungi. in this paper the cdna of the kiwi c ...201323830694
an r2r3 myb transcription factor determines red petal colour in an actinidia (kiwifruit) hybrid population.red colour in kiwifruit results from the presence of anthocyanin pigments. their expression, however, is complex, and varies among genotypes, species, tissues and environments. an understanding of the biosynthesis, physiology and genetics of the anthocyanins involved, and the control of their expression in different tissues, is required. a complex, the mbw complex, consisting of r2r3-myb and bhlh transcription factors together with a wd-repeat protein, activates anthocyanin 3-o-galactosyltransfe ...201323324587
structural and bioinformatic analysis of the kiwifruit allergen act d 11, a member of the family of ripening-related proteins.the allergen act d 11, also known as kirola, is a 17 kda protein expressed in large amounts in ripe green and yellow-fleshed kiwifruit. ten percent of all kiwifruit-allergic individuals produce ige specific for the protein. using x-ray crystallography, we determined the first three-dimensional structures of act d 11, produced from both recombinant expression in escherichia coli and from the natural source (kiwifruit). while act d 11 is immunologically correlated with the birch pollen allergen be ...201323969108
[establishment of high frequency regeneration via leaf explants of 'red sun' kiwifruit (actinidia chinensis)].a high efficient in vitro regeneration protocol was developed from leaf explants of the female 'red sun' kiwifruit (actinidia chinensis) and the multiplication coefficient and rooting rate of adventitious buds were also optimized. this method does not require formation of callus tissues which leads to somaclonal variations. the results show that the adventitious buds developing directly from explants tissue were noticed after 30 d of culture. the maximum regeneration frequency of adventitious bu ...201324701825
a randomised cross-over pharmacokinetic bioavailability study of synthetic versus kiwifruit-derived vitamin c.kiwifruit are a rich source of vitamin c and also contain numerous phytochemicals, such as flavonoids, which may influence the bioavailability of kiwifruit-derived vitamin c. the aim of this study was to compare the relative bioavailability of synthetic versus kiwifruit-derived vitamin c using a randomised cross-over pharmacokinetic study design. nine non-smoking males (aged 18-35 years) received either a chewable tablet (200 mg vitamin c) or the equivalent dose from gold kiwifruit (actinidia ch ...201324284610
draft genome of the kiwifruit actinidia chinensis.the kiwifruit (actinidia chinensis) is an economically and nutritionally important fruit crop with remarkably high vitamin c content. here we report the draft genome sequence of a heterozygous kiwifruit, assembled from ~140-fold next-generation sequencing data. the assembled genome has a total length of 616.1 mb and contains 39,040 genes. comparative genomic analysis reveals that the kiwifruit has undergone an ancient hexaploidization event (γ) shared by core eudicots and two more recent whole-g ...201324136039
a randomized steady-state bioavailability study of synthetic versus natural (kiwifruit-derived) vitamin c.whether vitamin c from wholefoods has equivalent bioavailability to a purified supplement remains unclear. we have previously showed that kiwifruit provided significantly higher serum and tissue ascorbate levels than synthetic vitamin c in a genetically vitamin c-deficient mouse model, suggesting a synergistic activity of the whole fruit. to determine if these results are translatable to humans, we carried out a randomized human study comparing the bioavailability of vitamin c from kiwifruit wit ...201324067392
biochemical and structural characterisation of dehydroquinate synthase from the new zealand kiwifruit actinidia chinensis.one of the novel aspects of kiwifruit is the presence of a high level of quinic acid which contributes to the flavour of the fruit. quinic acid metabolism intersects with the shikimate pathway, which is responsible for the de novo biosynthesis of primary and secondary aromatic metabolites. the gene encoding the enzyme which catalyses the second step of the shikimate pathway, dehydroquinate synthase (dhqs), from the new zealand kiwifruit actinidia chinensis was identified, cloned and expressed. a ...201323916589
kiwifruit: our daily prescription for health.kiwifruit are unequalled, compared with other commonly consumed fruit, for their nutrient density, health benefits, and consumer appeal. research into their health benefits has focussed on the cultivars actinidia deliciosa 'hayward' (green kiwifruit) and actinidia chinensis 'hort 16a', zespri(®) (gold kiwifruit). compared with other commonly consumed fruit, both green and gold kiwifruit are exceptionally high in vitamins c, e, k, folate, carotenoids, potassium, fibre, and phytochemicals acting i ...201323746068
total, soluble and insoluble oxalate contents of ripe green and golden kiwifruit.three bulk samples of two different cultivars of kiwifruit, green (actinidia deliciosa l.) and golden (actinidia chinensis l.) were bought ripe, ready to eat from a local market. the aim of the study was to determine the oxalate composition of each of the three fractions of kiwifruit, namely skin, pulp and seeds. the pulp consisted of 90.4% of the edible portion of the two cultivars while the skin and seeds made up a mean of 8.0% and 1.6% respectively. total oxalate was extracted with 2.0 m hcl ...201328239098
diversity and relative levels of actinidin, kiwellin, and thaumatin-like allergens in 15 varieties of kiwifruit (actinidia).in the last 30 years the incidence of kiwifruit allergy has increased with the three major allergenic proteins being identified as actinidin, kiwellin, and thaumatin-like protein (tlp). we report wide variation in the levels of actinidin and tlp in 15 kiwifruit varieties from the four most widely cultivated actinidia species. acidic and basic isoforms of actinidin were identified in actinidia deliciosa 'hayward' and actinidia arguta 'hortgem tahi', while only a basic isoform of actinidin was ide ...201323289429
proteomic changes in actinidia chinensis shoot during systemic infection with a pandemic pseudomonas syringae pv. actinidiae strain.a pandemic, very aggressive population of pseudomonas syringae pv. actinidiae is currently causing severe economic losses to kiwifruit crops worldwide. upon leaf attack, this gram-negative bacterium systemically reaches the plant shoot in a week period. in this study, combined 2-de and nanolc-esi-lit-ms/ms procedures were used to describe major proteomic changes in actinidia chinensis shoot following bacterial inoculation in host leaf. a total of 117 differentially represented protein spots were ...201323099348
metabolic profiling and outer pericarp water state in zespri, ci.gi, and hayward kiwifruits.the metabolic profiling of aqueous extracts of zespri gold ( actinidia chinensis ) and ci.gi (a controlled crossbreed from different species of actinidia deliciosa ) kiwifruits and the water state of the outer pericarp of entire fruits were monitored over the season by means of high-field nmr spectroscopy and t(2) relaxation time measurements, respectively, and compared with the corresponding ones of hayward kiwifruits previously investigated. a more complete assignment of the (1)h spectrum with ...201323083310
green and gold kiwifruit peel ethanol extracts potentiate pentobarbital-induced sleep in mice via a gabaergic mechanism.kiwifruit is one of the most popular fruits worldwide, and it has various biological properties, including antioxidant, anti-allergic, and cardiovascular protective effects. the peel of kiwifruit, which is a by-product of processing, is a good source of flavonoids; however, its bioactivity has not been widely investigated. in this study, we evaluated the hypnotic effects of green (grpe, actinidia deliciosa) and gold (gope, actinidia chinensis) kiwifruit peel ethanol extracts and their solvent fr ...201323017407
evaluation of antioxidant and antiproliferative properties of three actinidia (actinidia kolomikta, actinidia arguta, actinidia chinensis) extracts in vitro.the total phenolic content, total flavonoid content, vitamin c content, and antioxidant activities of ethanol extracts from different kiwifruit varieties (actinidia kolomikta, actinidia arguta, actinidia chinensis) were determined in this study. multiple scavenging activity assays including the hydroxyl radical, o(2) (-)·radical, dpph, and the abts(+) radical scavenging activity assays were used to identify the antioxidant activities of actinidia extracts. the cell viability of hepg2 and ht-29 c ...201222754311
the effect of 2n gametes on sex ratios in actinidia.sex can sometimes lead to complications. in some crops, 2n gametes have been exploited by plant breeders to transfer genetic variation between taxa of different ploidy levels. however, their role and use in dioecious genera have received relatively little attention. in the dioecious genus actinidia (kiwifruit), seedling populations usually segregate equally for females and males as sex is determined by an xx female/xy male system. while fertilization involving 2n egg cells is not expected to aff ...201222739644
bioavailability of vitamin c from kiwifruit in non-smoking males: determination of 'healthy' and 'optimal' intakes.vitamin c is an essential nutrient in humans and must be obtained through the diet. the aim of this study was to determine vitamin c uptake in healthy volunteers after consuming kiwifruit (actinidia chinensis var. hort. 16a), and to determine the amount of fruit required to raise plasma vitamin c to 'healthy' (i.e. >50 µmol/l) and 'optimal' or saturating levels (i.e. >70 µmol/l). leucocyte and urinary vitamin c levels were also determined. a total of fifteen male university students with below a ...201225191543
consumption of gold kiwifruit reduces severity and duration of selected upper respiratory tract infection symptoms and increases plasma vitamin c concentration in healthy older adults.in the elderly, immunosenescence and malnourishment can contribute to increased risk and severity of upper respiratory tract infections (urti). gold kiwifruit (actinidia chinensis 'hort16a') contains nutrients important for immune function and mitigation of symptoms of infection, including vitamins c and e, folate, polyphenols and carotenoids. the objective of the present study was to evaluate whether regular consumption of gold kiwifruit reduces symptoms of urti in older people, and determine t ...201222172428
identification, functional characterization, and regulation of the enzyme responsible for floral (e)-nerolidol biosynthesis in kiwifruit (actinidia chinensis).flowers of the kiwifruit species actinidia chinensis produce a mixture of sesquiterpenes derived from farnesyl diphosphate (fdp) and monoterpenes derived from geranyl diphosphate (gdp). the tertiary sesquiterpene alcohol (e)-nerolidol was the major emitted volatile detected by headspace analysis. contrastingly, in solvent extracts of the flowers, unusually high amounts of (e,e)-farnesol were observed, as well as lesser amounts of (e)-nerolidol, various farnesol and farnesal isomers, and linalool ...201222162874
vascular functioning and the water balance of ripening kiwifruit (actinidia chinensis) berries.indirect evidence suggests that water supply to fleshy fruits during the final stages of development occurs through the phloem, with the xylem providing little water, or acting as a pathway for water loss back to the plant. this inference was tested by examining the water balance and vascular functioning of ripening kiwifruit berries (actinidia chinensis var. chinensis 'hort16a') exhibiting a pre-harvest 'shrivel' disorder in california, and normal development in new zealand. dye labelling and m ...201222155631
phylogenetic relationships among global populations of pseudomonas syringae pv. actinidiae.abstract pseudomonas syringae pv. actinidiae, the causal agent of canker in kiwifruit (actinidia spp.) vines, was first detected in japan in 1984, followed by detections in korea and italy in the early 1990s. isolates causing more severe disease symptoms have recently been detected in several countries with a wide global distribution, including italy, new zealand, and china. in order to characterize p. syringae pv. actinidiae populations globally, a representative set of 40 isolates from new zea ...201222877312
bacterial canker on kiwifruit in italy: anatomical changes in the wood and in the primary infection sites.the bacterial canker of kiwifruit caused by pseudomonas syringae pv. actinidiae is a severe threat to kiwifruit production worldwide. many aspects of p. syringae pv. actinidiae biology and epidemiology still require in-depth investigation. the infection by and spread of p. syringae pv. actinidiae in xylem and phloem was investigated by carrying out artificial inoculation experiments with histological and dendrochronological analyses of naturally diseased plants in italy. we found that the bacter ...201222713076
pseudomonas syringae pv. actinidiae: a re-emerging, multi-faceted, pandemic pathogen.pseudomonas syringae pv. actinidiae is the causal agent of bacterial canker of green-fleshed kiwifruit (actinidia deliciosa) and yellow-fleshed kiwifruit (a. chinensis). a recent, sudden, re-emerging wave of this disease has occurred, almost contemporaneously, in all of the main areas of kiwifruit production in the world, suggesting that it can be considered as a pandemic disease. recent in-depth genetic studies performed on p. syringae pv. actinidiae strains have revealed that this pathovar is ...201222353258
meiotic chromosome pairing in actinidia chinensis var. deliciosa.polyploids are defined as either autopolyploids or allopolyploids, depending on their mode of origin and/or chromosome pairing behaviour. autopolyploids have chromosome sets that are the result of the duplication or combination of related genomes (e.g., aaaa), while allopolyploids result from the combination of sets of chromosomes from two or more different taxa (e.g., aabb, aabbcc). allopolyploids are expected to show preferential pairing of homologous chromosomes from within each parental sub- ...201223196697
low-temperature-modulated fruit ripening is independent of ethylene in 'sanuki gold' kiwifruit.fruit ripening in response to treatments with propylene, 1-methycyclopropene (1-mcp), and low temperature was characterized in 'sanuki gold' kiwifruit, actinidia chinensis planch. propylene treatment immediately induced rapid fruit softening, increased ac-pg (polygalacturonase) and ac-exp (expansin) mrna accumulation, and stimulated an increase in the soluble solid concentration (ssc) and a decrease in titratable acidity (ta). after 3 d exposure to propylene, ethylene production and ac-pl (pecta ...201222058408
mapping, complementation, and targets of the cysteine protease actinidin in kiwifruit.cysteine proteases (cps) accumulate to high concentration in many fruit, where they are believed to play a role in fungal and insect defense. the fruit of actinidia species (kiwifruit) exhibit a range of cp activities (e.g. the actinidia chinensis variety yellowa shows less than 2% of the activity of actinidia deliciosa variety hayward). a major quantitative trait locus for cp activity was mapped to linkage group 16 in a segregating population of a. chinensis. this quantitative trait locus coloc ...201222039217
induced polyploidy dramatically increases the size and alters the shape of fruit in actinidia chinensis.some otherwise promising selections of actinidia chinensis (kiwifruit) have fruit that are too small for successful commercialization. we have therefore made the first detailed study in diploid kiwifruit of the effects of chromosome doubling induced by colchicine on fruit size, shape and crop loading.201221980192
a novel polygalacturonase-inhibiting protein (pgip) from lathyrus sativus l. seeds.polygalacturonase-inhibiting proteins (pgips) are extracellular plant proteins bound to the plant cell wall containing leucine-rich repeats (lrr). they play an important role in plant defence being able to inhibit fungal endopolygalacturonases (epgs), the first enzymes secreted by phytopathogenic fungi during plant infection. in the present work, a novel pgip (lspgip) has been isolated from lathyrus sativus seeds. lspgip exhibited an inhibitory activity towards epgs from aspergillus niger and rh ...201222762184
functional properties of some new zealand fruit extracts towards selected probiotic and pathogenic bacteria.the functional properties of aqueous extracts of four fruits commonly consumed in new zealand, namely blueberry, strawberry, green kiwifruit and feijoa, towards the growth of probiotic and pathogenic bacteria were investigated in this study. a concentration range of 0.1-30.0 g/l was evaluated in a high-throughput assay to determine their effects. in general, the four fruit extracts exerted growth-stimulating effects on the probiotic bacteria, except with bifidobacterium longum, whereas they were ...201222968373
in vitro utilization of gold and green kiwifruit oligosaccharides by human gut microbial populations.we examined the effects of whole kiwifruit on gut microbiota using an in vitro batch model of gastric-ileal digestion and colonic fermentation. faecal fermentations of gold and green kiwifruit, inulin and water (control) digests were performed for up to 48 h. as compared to the control, gold and green kiwifruit increased bifidobacterium spp. by 0.9 and 0.8 log(10) cfu/ml, respectively (p < 0.001), and the bacteroides-prevotella-porphyromonas group by 0.4 and 0.5 log(10) cfu/ml, respectively. inu ...201222576129
in vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit.higher plants possess several mechanisms of defense against plant pathogens. proteins actively synthesized in response to those stresses are called defense-related proteins which, among others, include certain protease inhibitors. it is of particular relevance to investigate plant natural defense mechanisms for pathogen control which include cystatins-specific inhibitors of cysteine proteases.201222653546
kiwifruit decreases blood pressure and whole-blood platelet aggregation in male smokers.lifestyle modifications to reduce risk factors for cardiovascular diseases such as blood pressure (bp) and smoking have been emphasized. fruits and vegetables may modify such risk factors. the major aim of this randomized, controlled trial was to investigate the effects of (1) kiwifruits and (2) an antioxidant-rich diet compared with (3) a control group on bp and platelet aggregation (that is, whole-blood platelet aggregation) after 8 weeks in male smokers (age 44-74 years, n=102). the kiwifruit ...201222258209
analytical determination of bioactive compounds as an indication of fruit quality.the aim of this investigation was to determine the bioactive compounds in kiwifruit as an indication of quality after extraction using methanol and ethyl acetate. using ftir and three-dimensional fluorescence spectroscopy and electrospray ionization/ms, the contents of polyphenols, flavonoids, flavanols, and tannins, and the level of the antioxidant activity by 2, 2-azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) diammonium salt, 1, 1-diphenyl-2-picrylhydrazyl, ferric-reducing/ antioxidant p ...201223451390
in vitro antibacterial activity of cysteine protease inhibitor from kiwifruit (actinidia deliciosa).the need for replacing traditional pesticides with alternative agents for the management of agricultural pathogens is rising worldwide. in this study, a cysteine proteinase inhibitor (cpi), 11 kda in size, was purified from green kiwifruit to homogeneity. we examined the growth inhibition of three plant pathogenic gram-negative bacterial strains by kiwi cpi and attempted to elucidate the potential mechanism of the growth inhibition. cpi influenced the growth of phytopathogenic bacteria agrobacte ...201224426085
pseudomonas syringae pv. actinidiae (psa) isolates from recent bacterial canker of kiwifruit outbreaks belong to the same genetic lineage.intercontinental spread of emerging plant diseases is one of the most serious threats to world agriculture. one emerging disease is bacterial canker of kiwi fruit (actinidia deliciosa and a. chinensis) caused by pseudomonas syringae pv. actinidiae (psa). the disease first occurred in china and japan in the 1980s and in korea and italy in the 1990s. a more severe form of the disease broke out in italy in 2008 and in additional countries in 2010 and 2011 threatening the viability of the global kiw ...201222590555
reactive oxygen species are involved in pollen tube initiation in kiwifruit.the role of reactive oxygen species (ros) during pollen tube growth has been well established, but its involvement in the early germination stage is poorly understood. ros production has been reported in germinating tobacco pollen, but evidence for a clear correlation between ros and germination success remains elusive. here, we show that ros are involved in germination and pollen tube formation in kiwifruit. using labelling with dihydrofluorescein diacetate (h(2) fda) and nitroblue tetrazolium ...201221973108
composition of sugars, organic acids, and total phenolics in 25 wild or cultivated berry species.sugars, organic acids, and total phenolic content in fruit of 25 wild and cultivated berry species were identified and quantified with high-performance liquid chromatograph. the composition of sugars, organic acids, and total phenolic compounds in various species of vaccinium, rubus, ribes, and fragaria genus was evaluated. additonally, total phenolics of less known berry species of the morus, amelanchier, sorbus, sambucus, rosa, lycium, actinidia, and aronia genus were determined in wild growin ...201222924969
immunomodulatory and therapeutic potentials of herbal, traditional/indigenous and ethnoveterinary medicines.herbs/botanical plants are considered as god's gift to human beings in the form of natural medicines, like the one well known "sanjeevani booti" described in hindu mythology. the traditional and ethno-veterinary practices have been in use for centuries, transferring the knowledge from generation to generation and they are accessible, easy to prepare and administer, with little or no cost at all. even though the modern developments in therapeutic field brought about a rapid decline in traditional ...201224175417
applications of nmr metabolomics to the study of foodstuffs: truffle, kiwifruit, lettuce, and sea bass.in this review, four examples of the nmr metabolomic approach to foodstuff investigation are reported. different types of foodstuff of different origin (namely truffle, kiwifruit, lettuce, and sea bass), with different metabolite composition, processing, and storage procedures have been chosen to demonstrate the versatility and potentiality of nmr in the foodstuff analysis. fundamental aspects of nmr methodology such as sample preparation, metabolites extraction, quantitative elaboration of spec ...201222887151
digested and fermented green kiwifruit increases human β-defensin 1 and 2 production in vitro.the intestinal mucosa is constantly exposed to a variety of microbial species including commensals and pathogens, the latter leaving the host susceptible to infection. antimicrobial peptides (amp) are an important part of the first line of defense at mucosal surfaces. human β-defensins (hbd) are amp expressed by colonic epithelial cells, which act as broad spectrum antimicrobials. this study explored the direct and indirect effects of green kiwifruit (kf) on human β-defensin 1 and 2 (hbd-1 and 2 ...201222872469
the control of chlorophyll levels in maturing kiwifruit.chlorophyll is present in many plant organs, including immature fruit where it is usually degraded during ripening. mature green kiwifruit (actinidia deliciosa) are an exception, with high concentrations of chlorophyll remaining in the fruit flesh. in gold-fleshed kiwifruit (a. chinensis), chlorophyll is degraded to colourless catabolites upon fruit ripening, leaving yellow carotenoids visible. we have identified candidate genes for the control of chlorophyll degradation in kiwifruit and examine ...201222843245
kiwifruit floral gene apetala2 is alternatively spliced and accumulates in aberrant indeterminate flowers in the absence of mir172.in arabidopsis, the identity of perianth and reproductive organs are specified by antagonistic action of two floral homeotic genes, apetala2 (ap2) and agamous (ag). ap2 is also negatively regulated by an evolutionary conserved interaction with a microrna, mir172, and has additional roles in general plant development. a kiwifruit gene with high levels of homology to ap2 and ap2-like genes from other plant species was identified. the transcript was abundant in the kiwifruit flower, particularly pe ...201222290408
physiological and proteomic approaches to address the active role of ozone in kiwifruit post-harvest ripening.post-harvest ozone application has recently been shown to inhibit the onset of senescence symptoms on fleshy fruit and vegetables; however, the exact mechanism of action is yet unknown. to characterize the impact of ozone on the post-harvest performance of kiwifruit (actinidia deliciosa cv. 'hayward'), fruits were cold stored (0 °c, 95% relative humidity) in a commercial ethylene-free room for 1, 3, or 5 months in the absence (control) or presence of ozone (0.3 μl l(-1)) and subsequently were al ...201222268155
actinidin, a protease from kiwifruit, induces changes in morphology and adhesion of t84 intestinal epithelial cells.actinidin belongs to the papain-like family of cysteine proteases and is a major kiwifruit allergen. in this study, the effect of actinidin on cellular morphology and adhesion of t84 intestinal cells was investigated. both rounding and detachment of t84 cells were observed upon actinidin treatment. the morphological changes and cell desquamation was protease-dependent, as well as time- and concentration-dependent. changes of intercellular adhesion and adhesion of epithelial cells to collagen upo ...201222257750
evaluation of ige reactivity of active and thermally inactivated actinidin, a biomarker of kiwifruit allergy.actinidin, an abundant cysteine protease from kiwifruit, is a specific biomarker of isolated allergy to kiwifruit. this study evaluates the ige-binding properties of biologically active and thermally inactivated actinidin. employing two different activity assays (caseinolytic assay and zymogram with gelatin) we showed that actinidin obtained from kiwifruit extract under native conditions represents a mixture of inactive and active enzyme. the structural integrity of actinidin was confirmed by sd ...201222227218
kiwifruit (actinidia deliciosa) changes intestinal microbial profile.kiwifruit is high in pectic polysaccharides and dietary fiber. this study aimed to find out how the ingestion of kiwifruit will affect intestinal microbiota populations, namely lactobacillus, bacteroides, clostridium, bifidobacterium, and enterococcus.201223990838
measurement of bremsstrahlung radiation for in vivo monitoring of 14c tracer distribution between fruit and roots of kiwifruit (actinidia arguta) cuttings.in vivo measurements of (14)c tracer distribution have usually involved monitoring the β(-) particles produced as (14)c decays. these particles are only detectable over short distances, limiting the use of this technique to thin plant material. in the present experiments, x-ray detectors were used to monitor the bremsstrahlung radiation emitted since β(-) particles were absorbed in plant tissues. bremsstrahlung radiation is detectable through larger tissue depths. the aim of these experiments wa ...201222729822
organic and conventional kiwifruit, myths versus reality: antioxidant, antiproliferative, and health effects.comparison between organic and conventional kiwifruit cultivars 'hayward' and 'bidan', which was done by four radical scavenging assays, esi-ms, and dsc measurements, showed significant differences between the cultivars. such results were not estimated in kiwifruit growing under organic and conventional conditions. the extraction of bioactive compounds was done by two different methods: sequential extraction with ethyl acetate followed by methanol and maceration with methanol and ethyl acetate. ...201222712722
evaluation of pre-rigor injection of beef with proteases on cooked meat volatile profile after 1 day and 21 days post-mortem storage.this research was carried out to determine the effects of pre-rigor injection of beef semimembranosus muscle with nine proteases from plant and microbial sources, on the volatile profile of cooked beef after 1 day and 21 days post-mortem (pm) storage using solid-phase microextraction gas chromatography mass spectrometry analysis. a total of 23 aldehydes, 5 ketones, 3 furans, 8 nitrogen and sulphur compounds, 4 alkanes, 7 alcohols and 6 terpenes were detected. eleven volatile compounds characteri ...201222682685
a functional pectin methylesterase inhibitor protein (solypmei) is expressed during tomato fruit ripening and interacts with pme-1.a pectin methylesterase inhibitor (solypmei) from tomato has been identified and characterised by a functional genomics approach. solypmei is a cell wall protein sharing high similarity with actinidia deliciosa pmei (adpmei), the best characterised inhibitor from kiwi. it typically affects the activity of plant pectin methylesterases (pmes) and is inactive against a microbial pme. solypmei transcripts were mainly expressed in flower, pollen and ripe fruit where the protein accumulated at breaker ...201222610346
development and validation of a direct competitive monoclonal antibody-based immunoassay for the sensitive and selective analysis of the phytoregulator forchlorfenuron.forchlorfenuron is a synthetic phytohormone with cytokinin-like activity used worldwide as a plant growth regulator to increase fruit size in a number of crops, mostly in kiwifruit and grape vines. a monoclonal antibody-based enzyme-linked immunosorbent assay (elisa) for the determination of forchlorfenuron has been characterized and optimized. the selected immunoreagents afforded a highly selective assay with a limit of detection of 10 ng l(-1) in buffer. this direct competitive elisa was valid ...201222526663
the evaluation of chinese therapeutic food for the treatment of moderate dyslipidemia.the clinical efficacy of the chinese therapeutic food (specifically hawthorn fruit and chinese kiwifruit-extract compound) on dyslipidemia was evaluated in this placebo-controlled, double blind, paired clinical trial conducted in melbourne, australia. forty-three participants diagnosed with moderate dyslipidemia and met the study criteria were randomly assigned to group a or b, with baseline characteristics matched. twenty-seven participants completed all the tests, the blood lipid profile inclu ...201222474507
anaphylactic shock due to kiwifruit.a 44-year-old male patient was admitted to our emergency department (ed) with an episode of severe anaphylaxis displaying generalized urticaria and dyspnea 1 hour after consuming a kiwifruit. initially, the patient reported discrete itching of his abdominal skin and was in moderate respiratory distress. the patient's wheal response and itch were attenuated 30 minutes after emergency treatment with intravenous antianaphylaxis drugs. however, he had symptoms of the chest distress, dizzy, and dysph ...201222386357
a report from the american heart association scientific sessions 2011 (november 12-16, 2011, orlando, florida, usa).diet and lifestyle have a direct impact on cardiovascular health and may help improve the condition in many patients suffering from or at risk for cardiac diseases, and remote and in-person behavioral interventions were shown to prompt weight loss in obese individuals (appel, l.j., late-breaking clinical trial presentation), thus helping reduce the cardiovascular risk. in that regard, and as an example, kiwifruit was noted to lower 24-hour blood pressure (svendsen, m. et al., abst 16175), wherea ...201222384460
modelling chlorophyll fluorescence of kiwi fruit (actinidia deliciosa).kiwi fruit displays chlorophyll fluorescence. a physical model was developed to reproduce the observed original fluorescence for the whole fruit, from the emission of the different parts of the kiwi fruit. the spectral distribution of fluorescence in each part of the fruit, was corrected to eliminate distortions due to light re-absorption and it was analyzed in relation to photosystem ii-photosystem i ratio. kiwi fruit also displays variable chlorophyll-fluorescence, similar to that observed fro ...201222337099
studies related to norway spruce galactoglucomannans: chemical synthesis, conformation analysis, nmr spectroscopic characterization, and molecular recognition of model compounds.galactoglucomannan (ggm) is a polysaccharide mainly consisting of mannose, glucose, and galactose. ggm is the most abundant hemicellulose in the norway spruce (picea abies), but is also found in the cell wall of flax seeds, tobacco plants, and kiwifruit. although several applications for ggm polysaccharides have been developed in pulp and paper manufacturing and the food and medical industries, attempts to synthesize and study distinct fragments of this polysaccharide have not been reported prev ...201223008171
an efficient method for purification of nonspecific lipid transfer protein-1 from rice seeds using kiwifruit actinidin proteolysis and ion exchange chromatography.plant nonspecific lipid transfer proteins are small basic proteins that transport phospholipids between membranes and are subdivided into two subfamilies, nsltp(1) (9 kda) and nsltp(2) (7 kda). ltps have potential application in the defense reactions against pathogens and the drug delivery systems. many efforts have been made for purification of different nsltps from various plants; however, most of them used successive purification procedures. we have developed a relatively simple and efficient ...201222987614
kiwifruit remnants from digestion in vitro have functional attributes of potential importance to health.the physicochemical properties of plant cell walls in the gut are important in modulating processes that influence health. we investigated the physicochemical properties of kiwifruit cell walls digested under gastric and gastroileal conditions in vitro. soluble and insoluble undigested polymer fractions were measured, the hydration properties of the digested pulp, and the capacity to retard diffusion and mixing in a simulated small intestinal segment. undigested polymer (dietary fibre) fractions ...201222980789
hplc-msn identification and quantification of flavonol glycosides in 28 wild and cultivated berry species.berries and red fruits are rich dietary sources of polyphenols with reported health benefits. more than 50 different flavonols (glycosides of quercetin, myricetin, kaempferol, isorhamnetin, syringetin and laricitrin) have been detected and quantified with hplc-ms(n) in fruits of blueberry, bilberry, cranberry, lingonberry, eastern shadbush, japanese wineberry, black mulberry, chokeberry, red, black and white currants, jostaberry, red and white gooseberry, hardy kiwifruit, goji berry, rowan, dog ...201222980782
characterisation of bound volatile compounds of a low flavour kiwifruit species: actinidia eriantha.aroma compounds in fruit are known to occur in free and glycosidically bound forms. the bound volatile fraction of a low flavour kiwifruit species, actinidia eriantha, was studied. the fruit have a bland and grassy flavour. glycosidic precursors were isolated from juice by adsorption onto an amberlite xad-2 column. after enzymatic hydrolysis with rapidase ar2000, the released aglycones were analysed by gc-ms. alcohols, terpenoids and phenolics were the most numerously represented compound classe ...201223107675
characterizing kiwifruit carbohydrate utilization in vitro and its consequences for human faecal microbiota.it is well accepted that our gut bacteria have coevolved with us in relation to our genetics, diet and lifestyle and are integrated metabolically with us to affect our gut health adversely or beneficially. "who is there" may vary quite widely between individuals, as might "how they do it", but "what they make" may be less variable. many different individual species of bacteria can perform the same saccharolytic functions and so the availability of substrate (host or diet-derived) along with the ...201223072625
topical effectiveness of kiwifruit versus fibrinolysin ointment on removal of necrotic tissue of full-thickness burns in male rats.formation of necrotic tissues is a major issue affecting treatment of full-thickness burns. this study was designed to compare topical effectiveness of applying kiwifruit versus fibrinolysin on removal of necrotic tissue of burns. ten adult male wistar rats were randomly assigned to three groups. for group 1, the right-side wounds were treated with kiwifruit and the other side with fibrinolysin. for group 2, the wounds on the right side were treated with kiwifruit or fibrinolysin, and the left s ...201223210763
fruit transpiration in kiwifruit: environmental drivers and predictive model.in most fruit crops, storage quality varies greatly between regions and seasons, causing significant commercial loss. understanding the sources of this variability will contribute to the knowledge of fruit developmental physiology and may also benefit commercial fruit production via altered managements that reduce it or forecasts that predict it. a causal-chain relationship is proposed to help elucidate the sources of variability in fruit storage quality: the weather →(i)→ fruit transpiration →( ...201223136639
conservation and divergence of four kiwifruit svp-like mads-box genes suggest distinct roles in kiwifruit bud dormancy and flowering.mads-box genes similar to arabidopsis short vegetative phase (svp) have been implicated in the regulation of flowering in annual species and bud dormancy in perennial species. kiwifruit (actinidia spp.) are woody perennial vines where bud dormancy and out-growth affect flower development. to determine the role of svp-like genes in dormancy and flowering of kiwifruit, four mads-box genes with homology to arabidopsis svp, designated svp1, svp2, svp3, and svp4, have been identified and analysed in ...201222071267
effect of acid fruit juices combined with electric or sonic toothbrushing on root dentin permeability--an in vitro study.the purpose of this in vitro study was to quantify the alterations on human root dentin permeability after exposure to different acid fruit juices and to evaluate the effect of toothbrushing with electric or sonic toothbrush after acid exposure. the root dentin of 50 extracted third molars was exposed with a high speed bur. crowns were sectioned above the cementoenamel junction and root fragments were used to prepare dentin specimens. specimens were randomly assigned to 5 groups according to the ...201223338258
cutin plays a role in differentiation of endosperm-derived callus of kiwifruit.cutin fluorescence, after auramine o treatment, was detected on the surface of organogenic areas (protuberances) of endosperm derived callus induced on murashige and skoog medium with thidiazuron (0.5 mg l(-1)) in darkness. electron micrographs of the protuberances revealed cuticle, visible as a dark-staining layer, and amorphous waxes on the cell wall. in some cases the cells of the epidermis-like layer and shoot buds at early stages of development showed thick and characteristically wavy cutin ...201121750903
towards aspect-oriented functional--structural plant modelling.functional-structural plant models (fspms) are used to integrate knowledge and test hypotheses of plant behaviour, and to aid in the development of decision support systems. a significant amount of effort is being put into providing a sound methodology for building them. standard techniques, such as procedural or object-oriented programming, are not suited for clearly separating aspects of plant function that criss-cross between different components of plant structure, which makes it difficult t ...201121724653
proteomic changes and molecular effects associated with cr(iii) and cr(vi) treatments on germinating kiwifruit pollen.the present study is aimed at identifying molecular changes elicited by cr(iii) and cr(vi) on germinating kiwifruit pollen. to address this question, comparative proteomic and dna laddering analyses were performed. while no genotoxic effect was detected, a number of proteins whose accumulation levels were altered by treatments were identified. in particular, the upregulation of some proteins involved in the scavenging response, cell redox homeostasis and lipid synthesis could be interpreted as a ...201121708391
effect of kiwifruit consumption on sleep quality in adults with sleep problems.numerous studies have revealed that kiwifruit contains many medicinally useful compounds, among which antioxidants and serotonin may be beneficial in the treatment of the sleep disorders. the aim of this study was to evaluate the effects of kiwifruit on sleep patterns, including sleep onset, duration, and quality. in this study, we applied a free-living, self-controlled diet design. twenty-four subjects (2 males, 22 females) 20 to 55 years of age consumed 2 kiwifruits 1 hour before bedtime night ...201121669584
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