Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| A comparison of three rotary systems and hand instrumentation technique for the elimination of Enterococcus faecalis from the root canal. | The aim of this study was to compare the in vitro reduction of a bacterial population in a root canal by mechanical instrumentation using three rotary systems and hand instrumentation technique. The root canals contaminated with a suspension of Enterococcus faecalis were instrumented using ProTaper, K3, HeroShaper and K-file hand instrumentation technique. Later the root canals were sampled. After serial dilutions, samples were incubated in culture media for 24 h. Bacterial colonies were counted ... | 2011 | 22117720 |
| determining the disinfection of textiles in compressed carbon dioxide using various indicator microbes. | aims: this paper presents a research on the disinfection efficiency of inoculated textile swatches by compressed carbon dioxide, an environmental friendly way to disinfect textiles as opposed to the conventional laundering procedures using water. the disinfection efficiency was determined by using the following microbes inoculated on cotton test fabrics: enterococcus faecium, enterobacter aerogenes and candida albicans. methods and results: the experiments were performed using the high pressur ... | 2011 | 22151328 |
| structural biology. up close with membrane lipid-protein complexes. | 2011 | 22021848 | |
| Daily measures of microbes and human health at a non-point source marine beach. | Studies evaluating the relationship between microbes and human health at non-point source beaches are necessary for establishing criteria which would protect public health while minimizing economic burdens. The objective of this study was to evaluate water quality and daily cumulative health effects (gastrointestinal, skin, and respiratory illnesses) for bathers at a non-point source subtropical marine recreational beach in order to better understand the inter-relationships between these factors ... | 2011 | 21976192 |
| additive effect of a diode laser on the antibacterial activity of 2.5% naocl, 2% chx and mtad against enterococcus faecalis contaminating root canals: an in vitro study. | this in vitro study was performed to evaluate the effect of a diode laser and common disinfectants used in combination on mono-infected dental canals. one hundred and six single-rooted human premolars were prepared and contaminated with enterococcus faecalis. after two weeks of incubation, samples were divided into two experimental groups (n = 48) and two control groups (n = 5). in the first group, the teeth were rinsed for 5 min with either sterile saline, 2.5% naocl, or mtad, or for 1 min with ... | 2011 | 21959664 |
| potentially pathogenic bacteria and antimicrobial resistance in bioaerosols from cage-housed and floor-housed poultry operations. | background: antibiotics are used in animal confinement buildings, such as cage-housed (ch) and floor-housed (fh) poultry operations, to lower the likeliness of disease transmission. in fh facilities, antibiotics may also be used at sub-therapeutic levels for growth promotion. low levels of antibiotic create a selective pressure toward antimicrobial resistance (amr) in chicken fecal bacteria. objective: the objective of this study was to compare bacteria and amr genes in bioaerosols from ch and ... | 2011 | 22156572 |
| Hydrolytic fate of deoxynivalenol-3-glucoside during digestion. | Deoxynivalenol-3-ß-D-glucoside (D3G), a plant phase II metabolite of the Fusarium mycotoxin deoxynivalenol (DON), occurs in naturally contaminated wheat, maize, oat, barley and products thereof. Although considered as a detoxification product in plants, the toxicity of this substance in mammals is currently unknown. A major concern is the possible hydrolysis of the D3G conjugate back to its toxic precursor mycotoxin DON during mammalian digestion. We used in vitro model systems to investigate th ... | 2011 | 21878373 |
| Outbreak of vancomycin-resistant Enterococcus in a renal transplant unit. | 2011 | 21861018 | |
| fluoroquinolones, antimicrobial resistance and neutropenic cancer patients. | fluoroquinolone antibiotics are widely used in our communities and healthcare facilities. this review focuses upon the relationship between fluoroquinolone use and the rising prevalence in neutropenic cancer patients of multidrug resistant pathogens including methicillin-resistant staphylococcus aureus (mrsa), vancomycin-resistant enterococci, clostridium difficile, and aerobic gram-negative bacilli. | 2011 | 22001945 |
| agreement assessment of tigecycline susceptibilities determined by the disk diffusion and broth microdilution methods among commonly encountered resistant bacterial isolates: results from the tigecycline in-vitro surveillance in taiwan [tist] study, 2008-2010. | the tigecycline in-vitro surveillance in taiwan (tist) study, initiated in 2006, is a nationwide surveillance program designed to longitudinally monitor the in-vitro activity of tigecycline against commonly encountered drug-resistant bacteria. this study compared the in-vitro activity of tigecycline against 3,014 isolates of clinically important drug-resistant bacteria using the standard broth microdilution and disk diffusion methods. species studied included methicillin-resistant staphylococcus ... | 2011 | 22155819 |
| [Bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between April 2009 and March 2010]. | Bacteria isolated from surgical infections during the period from April 2009 to March 2010 were investigated in a multicenter study in Japan, and the following results were obtained. In this series, 671 strains including 16 strains of Candida spp. were isolated from 174 (79.1%) of 220 patients with surgical infections. Four hundred and eleven strains were isolated from primary infections, and 244 strains were isolated from surgical site infection. From primary infections, anaerobic Gram-negative ... | 2011 | 21861307 |
| Comparison of bacteria isolated from urinary tract infections in patients on transplant versus urologic wards. | Urinary tract infections (UTI) are the most common hospital infections. Complications include sepsis and shock. Immunosuppressed transplant surgery patients may experience loss of the graft due to UTI. The purpose of this study was to determine the main microorganisms responsible for UTIs among patients of transplant wards compared to urologic wards. Additionally, drug susceptibility profiles of the most frequent microorganisms were analyzed. | 2011 | 21996243 |
| purification and characterization of enterocin mc13 produced by a potential aquaculture probiont enterococcus faecium mc13 isolated from the gut of mugil cephalus. | a bacteriocin producer strain mc13 was isolated from the gut of mugil cephalus (grey mullet) and identified as enterococcus faecium. the bacteriocin of e. faecium mc13 was purified to homogeneity, as confirmed by tricine sodium dodecyl sulphate - polyacrylamide gel electrophoresis (sds-page). reverse-phase high-performance liquid chromatography (hplc) analysis showed a single active fraction eluted at 26 min, and matrix-assisted laser desorption ionization time of flight (maldi-tof) mass spectro ... | 2011 | 22112158 |
| antimicrobial resistance patterns to beta-lactams of gram-positive cocci isolated from bovine mastitis in lithuania. | the aim of the study was to isolate gram-positive cocci from cows with mastitis and to determine their resistance to beta-lactamic antibiotics. eight hundred and nine strains were isolated and identified as staphylococci (n=516), streptococci (n=199) and enterococci (n=94) from sub-clinical and clinical cases of bovine mastitis in lithuania. the most common causative agents of udder disease included: s. epidermidis (n=176), s. aureus (n=176), s. agalactiae (n=134), s. hyicus (136) and e. hirae ( ... | 2011 | 21957743 |
| Acanthamoeba polyphaga, a potential environmental vector for the transmission of food-borne and opportunistic pathogens. | The endosymbiotic relationship could represent for many bacteria an important condition favouring their spread in the environment and in foods. For this purpose we studied the behaviour of some food-borne and opportunistic pathogens (Listeria monocytogenes, Staphylococcus aureus, Enterococcus faecalis, Salmonella enterica serovar Enteritidis, Aeromonas hydrophila, Yersinia enterocolitica) when internalized in Acanthamoeba polyphaga. Our results confirm the capability of the bacteria tested to gr ... | 2011 | 21953544 |
| Method for the direct observation and quantification of survival of bacteria attached to negatively or positively charged surfaces in an aqueous medium. | The risk of groundwater contamination by microbial pathogens is linked to their survival in the subsurface. Although there is a large body of literature on the inactivation behavior of suspended (planktonic) microorganisms, little is known about the inactivation of bacteria when attached to sand grain surfaces in groundwater aquifers. The main goal of this study was to develop a fluorescence-based experimental technique for evaluating the extent of inactivation over time of bacteria adhered onto ... | 2011 | 21848280 |
| pan-european monitoring of susceptibility to human-use antimicrobial agents in enteric bacteria isolated from healthy food-producing animals. | objectives: to determine the antimicrobial susceptibility of escherichia coli, salmonella, campylobacter and enterococcus from cattle, pigs and chickens across the european union (eu) using uniform methodology. methods: intestinal samples (1624) were taken at slaughter across five eu countries. bacteria were isolated in national laboratories, whilst mics were determined in a central laboratory for key antimicrobials used in human medicine. clinical resistance was based on clsi breakpoints and de ... | 2011 | 22210758 |
| in vitro enterococcus faecalis biofilm formation on five adhesive systems. | objective: to determine the e. faecalis biofilm formation on the surface of five adhesive systems (as) and its relationship with roughness. study design: the formation of e. faecalis biofilms was tested on the surface of four dual-cure as: adhese dc, clearfil dc bond, futurabond dc and excite dsc and one light-cure antimicrobial as, clearfil protect bond, after 24 hours of incubation, using the mbec high-throughput device. results: e. faecalis biofilms grew on all the adhesives. the least growth ... | 2011 | 22143728 |
| Application of Baranyi function to model the antibacterial properties of solvent extract from Irish York cabbage against food spoilage and pathogenic bacteria. | Cabbage is a rich source of a number of bioactive compounds such as flavonoids, glucosinolates and their breakdown products which may have antibacterial, antioxidant and anticancer properties. Outer green leaves of York cabbage were extracted with 60% methanol, ethanol or acetone. Antibacterial activities of vacuum dried crude extracts were estimated against a number of Gram-positive and Gram-negative food spoilage and food pathogenic bacteria. The crude extracts showed a broad spectrum of antib ... | 2011 | 21954314 |
| Epidemiology of hospital-acquired urinary tract-related bloodstream infection at a university hospital. | Little is known about the epidemiology of nosocomial urinary tract-related bloodstream infection. In a case series from an academic medical center, Enterococcus (28.7%) and Candida (19.6%) species were the predominant microorganisms, which suggests a potential shift from gram-negative microorganisms. A case-fatality rate of 32.8% highlights the severity of this condition. | 2011 | 22011543 |
| Urinary tract infections in renal transplant recipients. | Urinary tract infections (UTIs) are most common infections in renal transplant recipients and are considered a potential risk factor for poorer graft outcomes. | 2011 | 21996206 |
| [Microbial diversity of a community for ensiling rice straw at low temperature and fermentation dynamics]. | To accelerate the conversion of rice straw into feeds in the low-temperature region, a microbial community was constructed by continuous enrichment cultivation. Microbial diversity and dynamics during the fermentation at 10 degrees C was analyzed. | 2011 | 22126081 |
| six-month incidence of bloodstream infections in intestinal transplant patients. | background: intestinal transplantation has emerged as an established treatment for life-threatening intestinal failure. the most common complication and cause of death is infection. risk of infection is highest during the first 6 months, as a consequence of maximal immunosuppression, greater than that required for any other organ allograft. methods: we performed a retrospective chart review of all (56) adult and pediatric (<18 years) small bowel transplant patients at our institution between nov ... | 2011 | 22093913 |
| N-O chemistry for antibiotics: discovery of N-alkyl-N-(pyridin-2-yl)hydroxylamine scaffolds as selective antibacterial agents using nitroso Diels-Alder and ene chemistry. | The discovery, syntheses, and structure-activity relationships (SAR) of a new family of heterocyclic antibacterial compounds based on N-alkyl-N-(pyridin-2-yl)hydroxylamine scaffolds are described. A structurally diverse library of ~100 heterocyclic molecules generated from Lewis acid-mediated nucleophilic ring-opening reactions with nitroso Diels-Alder cycloadducts and nitroso ene reactions with substituted alkenes was evaluated in whole cell antibacterial assays. Compounds containing the N-alky ... | 2011 | 21859126 |
| Evaluation of cleaning capacity and instrumentation time of manual, hybrid and rotary instrumentation techniques in primary molars. | Pinheiro SL, Araujo G, Bincelli I, Cunha R, Bueno C. Evaluation of cleaning capacity and instrumentation time of manual, hybrid and rotary instrumentation techniques in primary molars. International Endodontic Journal. ABSTRACT: Aim To compare the cleaning effectiveness of manual, hybrid and rotary instrumentation techniques in primary molar teeth. Methodology Fifteen primary molars were selected. After endodontic access, the teeth were immersed in a medium containing Enterococcus faecalis and ... | 2011 | 22188162 |
| [selection and spreading of antibiotic resistance in bacteria]. | use of an antibiotic may not only select for resistance against the agent itself, but may at the same time co-select for resistance against other antibiotics if resistance genes are linked on e.g. a plasmid. resistance plasmids may also carry genes mediating resistance against metals and disinfectants. therefore, abundant use of metals, e.g. copper and zinc for growth promotion in animals used for food, may also co-select for antibiotic resistance. the same applies to disinfectants, e.g. silver ... | 2011 | 22053807 |
| do dental impression materials play a role in cross contamination? | dentists are required to institute infectious control procedures. dental impression materials possessing antimicrobial properties may aid in reducing the risk of cross contamination since impression materials might play a role as carriers. the purpose of this study was to evaluate the antimicrobial properties of four impression materials. | 2011 | 22026004 |
| polycationic chitosan-conjugated photosensitizer for antibacterial photodynamic therapy(†). | the complex nature of bacterial cell membrane and structure of biofilm has challenged the efficacy of antimicrobial photodynamic therapy. this study was aimed to synthesize a polycationic chitosan-conjugated rose bengal (csrb) photosensitizer and test its antibiofilm efficacy on enterococcus faecalis (gram positive) and pseudomonas aeruginosa (gram negative) using photodynamic therapy. during experiments, csrb was tested along with an anionic photosensitizer rose bengal (rb) and a cationic photo ... | 2011 | 22044238 |
| is screening patients for antibiotic-resistant bacteria justified in the indian context? | infection with multi-antibiotic-resistant bacteria is a common clinical problem in india. in some countries and centres, screening patients to detect colonisation by these organisms is used to determine specific interventions such as decolonisation treatment, prophylactic antibiotics prior to surgical interventions or for selection of empirical antibiotic therapy, and to isolate patients so that transmission of these difficult to treat organisms to other patients could be prevented. in india, th ... | 2011 | 21860099 |
| high-level dietary fibre up-regulates colonic fermentation and relative abundance of saccharolytic bacteria within the human faecal microbiota in vitro. | background: health authorities around the world advise citizens to increase their intake of foods rich in dietary fibre because of its inverse association with chronic disease. however, a few studies have measured the impact of increasing mixed dietary fibres directly on the composition of the human gut microbiota. aims of the study: we studied the impact of high-level mixed dietary fibre intake on the human faec ... | 2011 | 21952691 |
| enterococcus plantarum sp. nov., isolated from plants. | a group of eight gram-positive, catalase negative bacterial strains isolated during a screening of enterococcal populations occurring on plants constituted a single cluster after the rep-pcr fingerprinting using the (gtg)5 primer and was separated from all known enterococcal species. 16s rrna gene sequence analysis of three selected representative strains classified the analysed group as members of the genus enterococcus and placed them to the enterococcus faecalis species phylogenetic group. fu ... | 2011 | 21856982 |
| a newly isolated streptomyces sp. cs392 producing three antimicrobial compounds. | with the aim of isolating new microbes capable of producing strong antimicrobial substances, strain cs392 was screened from 700 soil isolates preserved in our laboratory. the strain was related to genus streptomyces based on various characteristics. three highly active antimicrobial compounds, c1, c2 and c3, produced by the strain were purified by solvent extraction followed by silica gel column chromatography. these compounds were highly active against various gram-positive resistant bacteria s ... | 2011 | 21909674 |
| High-Resolution Melting Genotyping of Enterococcus faecium Based on Multilocus Sequence Typing Derived Single Nucleotide Polymorphisms. | We have developed a single nucleotide polymorphism (SNP) nucleated high-resolution melting (HRM) technique to genotype Enterococcus faecium. Eight SNPs were derived from the E. faecium multilocus sequence typing (MLST) database and amplified fragments containing these SNPs were interrogated by HRM. We tested the HRM genotyping scheme on 85 E. faecium bloodstream isolates and compared the results with MLST, pulsed-field gel electrophoresis (PFGE) and an allele specific real-time PCR (AS kinetic P ... | 2011 | 22195020 |
| Regional spread and control of vancomycin-resistant Enterococcus faecium and Enterococcus faecalis in Kyoto, Japan. | The purpose of this investigation was to control the post-outbreak prevalence of vancomycin-resistant enterococci (VRE) in the affected Kyoto region. The study period was from 2005 to 2010. Faecal samples were subjected to VRE screening, and vancomycin resistance genes were detected by polymerase chain reaction (PCR). The genotype was determined by pulsed-field gel electrophoresis (PFGE) of genomic DNA digested with SmaI and by multilocus sequence typing (MLST). A VRE control programme was estab ... | 2011 | 21968723 |
| Efficient reduction of nitrobenzene to aniline with a biocatalyzed cathode. | Nitrobenzene (NB) is a toxic compound that is often found as a pollutant in the environment. The present removal strategies suffer from high cost or slow conversion rate. Here, we investigated the conversion of NB to aniline (AN), a less toxic endproduct that can easily be mineralized, using a fed-batch bioelectrochemical system with microbially catalyzed cathode. When a voltage of 0.5 V was applied in the presence of glucose, 88.2 ± 0.60% of the supplied NB (0.5 mM) was transformed to AN within ... | 2011 | 21985580 |
| Development of an antibiogram in a long-term care facility. | To create an antibiogram-a profile of an organism's susceptibility/resistance to a panel of antibiotics- for a long-term care facility to assess the prevalence of resistance of bacteria present at the facility. | 2011 | 22079792 |
| microbial removal from the separated liquid fraction of anaerobically digested pig manure in meso-scale integrated constructed wetlands. | the aim was to investigate microbial removal from the liquid fraction of anaerobically digested pig manure in meso-scale integrated constructed wetlands (icw's) over a 13 month period. four treatments were investigated: t1 (standard), t2 (effluent recycling), t3 (high nutrient loading), and t4 (high flow rate). mean counts of yeasts and moulds and spore-forming bacteria were higher in t3 and t4 than in t1 and t2 (p<0.05). flow through the cells reduced mean counts of coliform, yeasts and moulds ... | 2011 | 21852121 |
| microbiological findings in endometrial specimen: our experience. | purpose: collection of an endometrial specimen for investigating infectious agents in the endometrial cavity is an invasive technique that is at times difficult and painful. in order to avoid the need for endometrial sampling in the cases of suspected or evident endometrial pathology, the aim of this study is to investigate the reliability of cervical cultures for detecting infectious agents present at the endometrial level, comparing the results between cervical cultures a ... | 2011 | 22113463 |
| chemical and antidiarrheal studies of plinia cauliflora. | plinia cauliflora (mart.) kausel, widespread in south america, has edible fruits, and its bark is commonly used against diarrhea and other disorders, on account of its astringency. because diarrhea is still one of the most important causes of illness and death among children in developing countries, where the population turns to traditional medicine for its treatment, the present study determined the composition of fruit and leaf extracts of p. cauliflora, analyzed the activity against diarrhea ... | 2011 | 21861716 |
| [high level of aminoglycoside resistance among enterococcus faecalis and enterococcus faecium strains]. | enterococcus sp. strains are believed as important reason of serious nosocomial infections currently. these infections are cured by using combination of beta-lactams and aminoglycosides for their treatment. enterococcus sp. resistant to high-level doses of aminoglycosides, beta-lactams and vancomycin are responsible for therapeutic failure. the aim of our study was to evaluate the incidence of isolation and susceptibility to antibiotics of hlar enterococcus sp. strains isolated between 2007 and ... | 2011 | 22184904 |
| successful control of vancomycin-resistant enterococcus faecium nosocomial outbreak in a teaching hospital in china. | in 2008, a vancomycin-resistant enterococci outbreak occurred across 2 intensive care units in our hospital. this outbreak prompted us to implement intensified control measures. we describe the molecular epidemiology of the outbreak, as well as the intervention strategies that resulted in successful control of the outbreak. | 2011 | 22036732 |
| Survival of host-associated Bacteroidales cells and their relationship with Enterococcus spp., Campylobacter jejuni, Salmonella Typhimurium and Adenovirus in freshwater microcosms as measured by PMA-qPCR. | The ideal host-associated genetic fecal marker would be capable of predicting the presence of specific pathogens of concern. Flow-through freshwater microcosms containing mixed feces and inocula of the pathogens Campylobacter jejuni, Salmonella Typhimurium and Adenovirus were placed at ambient temperature in the presence and absence of diurnal sunlight. The total Enterococcus DNA increased during the early periods (23 h) under sunlight exposure, even though cultivable Enterococcus and DNA in int ... | 2011 | 22139002 |
| enhanced antibacterial activity of bimetallic gold-silver core-shell nanoparticles at low silver concentration. | herein we report the development of bimetallic au@ag core-shell nanoparticles (nps) where gold nanoparticles (au nps) served as the seeds for continuous deposition of silver atoms on its surface. the core-shell structure and morphology were examined by uv-vis spectroscopy, transmission electron microscopy (tem), energy dispersive x-ray (edx) analysis and x-ray diffraction (xrd). the core-shell nps showed antibacterial activity against both gram negative (escherichia coli and pseudomonas aerugino ... | 2011 | 22057130 |
| successful therapy of treatment-emergent, non-clonal daptomycin-non-susceptible enterococcus faecium infections. | 2011 | 21846674 | |
| Bactericidal effects of two parameters of Er:YAG laser intracanal irradiation: ex-vivo study. | The success of endodontic treatment depends on the complete elimination of microorganisms from the root canal system, thus the search for new procedures to eliminate them is justified. The aim of this study was to assess bacterial reduction after intracanal irradiation with the Er:YAG laser. The canals of 70 extracted human maxillary canines were prepared up to file #40 using 1% NaOCl, irrigated with 17% EDTA, and then washed with physiological solution activated by ultrasound. The roots were st ... | 2011 | 22108869 |
| Rapid monitoring of Escherichia coli and Enterococcus spp. in bathing water using reverse transcription-quantitative PCR. | For accompanying actions to pollution crisis management of recreational waters, rapid methods providing a quantified faecal indicator which can be completed in less than half a day, are currently needed. Two sensitive rRNA-targeted Reverse Transcription-quantitative PCR (RT-qPCR) methods were developed for quantifying Escherichia coli and Enterococcus spp. cells in marine bathing waters. Threshold cycle (Ct) values were found to be linear with a target quantity over a 4log dynamic range (from 10 ... | 2011 | 21917514 |
| lower prevalence of antibiotic-resistant enterococci on u.s. conventional poultry farms that transitioned to organic practices. | background: in u.s. conventional poultry production, antimicrobials are used for therapeutic, prophylactic and non-therapeutic purposes. researchers have shown that this can select for antibiotic-resistant commensal and pathogenic bacteria on poultry farms and poultry-derived products. however, no u.s. studies have investigated on-farm changes in resistance as conventional poultry farms transition to organic practices and cease using antibiotics. objective: to evaluate the prevalence of antibiot ... | 2011 | 21827979 |
| adsorption of chlorhexidine on synthetic hydroxyapatite and in vitro biological activity. | the kinetic of chlorhexidine digluconate (chxdg) uptake from aqueous solution by hydroxyapatite (ha) was investigated by ultraviolet (uv) analysis performed in ha powder (uv-solid) after the chx adsorption. adsorption isotherm of chlorhexidine (chx) uptake was modeled by a combination of languimir and langmuir-freundlich mechanisms. strong molecule-molecule interactions and positive cooperativity predominated in the surface when chx concentration was above 8.6 ╬╝g(chx)/mg(ha). uv-solid spectra ( ... | 2011 | 21676601 |
| changes in vancomycin-resistant enterococcus faecium causing outbreaks in brazil. | enterococci have been implicated in severe human infections as a consequence of associated determinants of virulence and antimicrobial resistance. the majority of vancomycin-resistant enterococcus faecium (vre(fm)) connected to outbreaks worldwide pertains to the clonal complex 17 (cc17). in brazil, the majority of vre(fm) involved in outbreaks reported so far are not related to cc17. vre(fm) strains responsible for an outbreak and sporadic cases in hospitals located in the city of campinas, bra ... | 2011 | 21741112 |
| changes in bacterial resistance patterns in children with urinary tract infections on antimicrobial prophylaxis at university hospital in split. | we assessed prevalence and resistance of uropathogens on antimicrobial agents (aa) from urine cultures (uc) in children hospitalized with urinary tract infections (uti) at university hospital in split. | 2011 | 21709628 |
| evaluation of the heat-killed and dried cell preparation of enterococcus faecalis against villous atrophy in early-weaned mice and pigs. | early weaning induces villous atrophy in the small intestine (si) of piglets. oral administration of live lactic acid bacteria (lab) can improve villous shortening. in this study, we evaluated the oral administration of a heat-killed and dried cell preparation of enterococcus faecalis (a lab) strain ec-12 against villous atrophy in early-weaned mice (experiment 1) and pigs (experiments 2 and 3). twelve 16-days-old mice were divided into two groups in experiment 1: gavage of ec-12 (10 mg/kg body ... | 2011 | 21729210 |
| a new molecular approach based on qpcr for the quantification of fecal bacteria in contaminated marine sediments. | harbour sediments are periodically subjected to dredging operations and their management is mainly based on the assessment of the chemical contamination levels, but the potential risks posed by the presence of pathogenic microorganisms have been largely neglected. here we first developed new molecular protocols based on the use of real time quantitative pcr (qpcr), targeting both bacterial dna and the transcription product (rrna), for the identification and quantification of bacteria of fecal or ... | 2011 | 21839120 |
| detection of volatile compounds produced by microbial growth in urine by selected ion flow tube mass spectrometry (sift-ms). | selected ion flow tube-mass spectrometry has been used to measure the volatile compounds occurring in the headspace of urine samples inoculated with common urinary tract infection (uti)-causing microbes escherichia coli, proteus vulgaris, pseudomonas aeruginosa, staphylococcus aureus, staphylococcus epidermidis, klebsiella pneumoniae, enterococcus faecalis, or candida albicans. this technique has the potential to offer rapid and simple diagnosis of the causative agent of utis. | 2011 | 21741416 |
| investigation of the antimicrobial activity of rhaponticum (rhaponticum carthamoides d.c. iljin) and shrubby cinquefoil (potentilla fruticosa l.). | the aim of the study was to determine antimicrobial activity of rhaponticum and shrubby cinquefoil extracts. material and methods. ethanol extract from the leaves of rhaponticum (rhaponticum carthamoides d.c. iljin) and shrubby cinquefoil (potentilla fruticosa l.) was produced at the department of food technology, kaunas university of technology. the antimicrobial activity of the viscous extract or rhaponticum and shrubby cinquefoil was evaluated using standard microorganism cultures (bacteria s ... | 2011 | 21822040 |
| [the impact of uv radiation b and c in vitro on different of bacteria strains isolated from patients hospitalized in the warsaw medical university clinics]. | infections in human body caused by various microbes are a significant problem in modern medicine. special attention is put to infections of wounds, which are a significant threat to the life of patients. attempts to treat these wounds base mainly on the application of various chemical preparations (locally) and systematic antibiotic treatment. uv radiation, because of its anti-bacterial activity, appear a complementary issue in therapy. aim of the survey: the aim of this study was an examination ... | 2011 | 21735843 |
| Crystal structure of phosphopantetheine adenylyltransferase from Enterococcus faecalis in the ligand-unbound state and in complex with ATP and pantetheine. | Phosphopantetheine adenylyltransferase (PPAT) catalyzes the reversible transfer of an adenylyl group from ATP to 4'-phosphopantetheine (Ppant) to form dephospho-CoA (dPCoA) and pyrophosphate in the Coenzyme A (CoA) biosynthetic pathway. Importantly, PPATs are the potential target for developing antibiotics because bacterial and mammalian PPATs share little sequence homology. Previous structural studies revealed the mechanism of the recognizing substrates and products. The binding modes of ATP, A ... | 2011 | 21912874 |
| Photoactivated disinfection (PAD) in endodontics: an in vitro microbiological evaluation. | Purpose: The objective of the present study was the in vitro evaluation by MTT test of the antimicrobial effect of photoactivated disinfection (PAD) and, comparatively, of a conventional 5.25% NaOCl irrigating solution. Methods: Enterococcus faecalis, Streptococcus mutans and Streptococcus sanguis strains were selected for the test. Freshly extracted single-rooted human teeth were endodontically treated, inoculated with bacterial strains and then divided into different groups, each of them treat ... | 2011 | 22094571 |
| In situ disinfection of sewage contaminated shallow groundwater: a feasibility study. | Sewage-contaminated shallow groundwater is a potential cause of beach closures and water quality impairment in marine coastal communities. In this study we set out to evaluate the feasibility of several strategies for disinfecting sewage-contaminated shallow groundwater before it reaches the coastline. The disinfection rates of Escherichia coli (EC) and enterococci bacteria (ENT) were measured in mixtures of raw sewage and brackish shallow groundwater collected from a coastal community in southe ... | 2011 | 21906774 |
| RNA-mediated reciprocal regulation between two bacterial operons is RNase III dependent. | In bacteria, RNAs regulate gene expression and function via several mechanisms. An RNA may pair with complementary sequences in a target RNA to impact transcription, translation, or degradation of the target. Control of conjugation of pCF10, a pheromone response plasmid of Enterococcus faecalis, is a well-characterized system that serves as a model for the regulation of gene expression in bacteria by intercellular signaling. The prgQ operon, whose products mediate conjugation, is negatively regu ... | 2011 | 21954305 |
| [antimicrobial resistance monitoring in lower saxony (armin): first trends for mrsa, esbl-producing escherichia coli and vre from 2006 to 2010]. | antimicrobial resistance is one of the most important health topics of the past few years. to identify regional trends of antimicrobial resistance in inpatient and outpatient care, the governmental institute of public health of lower saxony (germany) launched the sentinel system armin (antimicrobial resistance monitoring in lower saxony). currently 9 laboratories participate as sentinel sites and contribute single case data of their microbiological results. data are presented by an interactive d ... | 2011 | 22113383 |
| Moving CLABSI prevention beyond the intensive care unit: risk factors in pediatric oncology patients. | Central line-associated bloodstream infections (CLABSIs) frequently complicate the use of central venous catheters (CVCs) among pediatric patients with cancer. Our objectives were to describe the microbiology and identify risk factors for hospital-onset CLABSI in this patient population. | 2011 | 22011534 |
| duodenal and faecal microbiota of celiac children: molecular, phenotype and metabolome characterization. | epidemiology of celiac disease (cd) is increasing. cd mainly presents in early childhood with small intestinal villous atrophy and signs of malabsorption. compared to healthy individuals, cd patients seemed to be characterized by higher numbers of gram-negative bacteria and lower numbers gram-positive bacteria. | 2011 | 21970810 |
| diagnosis of tuberculosis by trained african giant pouched rats and confounding impact of pathogens and microflora of the respiratory tract. | trained african giant-pouched rats (cricetomys gambianus) can detect mycobacterium tuberculosis and show potential for the diagnosis of tuberculosis (tb). however, rats' ability to discriminate between clinical sputum containing other mycobacterium spp. and nonmycobacterial species of the respiratory tract is unknown. it is also unknown whether nonmycobacterial species produce odor similar to m. tuberculosis and thereby cause the detection of smear-negative sputum. sputum samples from 289 subjec ... | 2011 | 22135255 |
| Trends in antimicrobial resistance in intensive care units in the United States. | Antimicrobial resistance and a paucity of new antimicrobial agents are ongoing challenges. This review focuses on the major epidemiologic trends and novel treatments, when available, for methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococcus, Clostridium difficile, and multidrug-resistant Gram-negative bacilli in the United States during 2010-2011. | 2011 | 21900766 |
| in vitro antibacterial activity of different endodontic irrigants. | abstract - aim: the objective of this study was to compare in vitro the antibacterial activity of tetraclean (mixture of doxycycline, citric acid and polypropylene glycol), niclor 5 (5.25% sodium hypochlorite solution), cloreximid (0.2% chlorhexidine and 0.2% cetrimide solution) and hydrogen peroxide 12 volumes on three endodontic pathogens associated with primary endodontic infections. enterococcus faecalis, streptococcus mutans and staphylococcus aureus strains were selected to evaluate the a ... | 2011 | 22051159 |
| Does the emergence of antibiotic resistance announce the return of the dark ages? | Antibiotic revolution changed the course of modern medicine, by decreasing mortality due to bacterial infections. However, bacteria have developed several ways of resistance against all antibiotics used. In view of the rise of resistance and of its dissemination to different bacterial families, new effective antibiotics are scare and the return in the pre-antibiotic era seems to be a reality for some parts of the world. This review revises the worrying phenomenon of antibiotic resistance focusin ... | 2011 | 22123562 |
| pseudonocardians a-c, new diazaanthraquinone derivatives from a deap-sea actinomycete pseudonocardia sp. scsio 01299. | pseudonocardians a-c (2-4), three new diazaanthraquinone derivatives, along with a previously synthesized compound deoxynyboquinone (1), were produced by the strain scsio 01299, a marine actinomycete member of the genus pseudonocardia, isolated from deep-sea sediment of the south china sea. the structures of compounds 1-4 were determined by mass spectrometry and nmr experiments (¹h, ¹³c, hsqc, and hmbc). the structure of compound 1, which was obtained for the first time from a natural source, wa ... | 2011 | 21892356 |
| in vitro evaluation of antimicrobial efficacy of endodontic irrigants. | the objective of this study was to compare in vitro, by mtt assay, the antimicrobial efficacy of niclor 5 (5% naocl solution), cloreximid (0.2% chlorhexidine and 0.2% cetrimide solution), 3% hydrogen peroxide and 17% edta against two microorganisms associated with primary endodontic infections. | 2011 | 22094574 |
| Characterization of temperature inducible promoters from a novel rolling circle replicating plasmid of Enterococcus faecium DJ1. | Limited studies have been performed on the characterization of small size plasmids of Enterococcus faecium with the intention of evaluating the strength of their promoters in Escherichia coli. The complete nucleotide sequence (3.825Kb) and structural organization of E. faecium DJ1 cryptic plasmid pNJAKD is presented. Seven promoter sequences from the pNJAKD plasmid of E. faecium have been identified. The regions coding for the putative promoters were either amplified using PCR based techniques o ... | 2011 | 22192842 |
| potential application of the nisin z preparation of lactococcus lactis w8 in preservation of milk. | aims: the aim of the study is to evaluate the effectiveness of the preparation of nisin z from lactococcus lactis w8-fermented milk in controlling the growth of spoilage bacteria in pasteurized milk. methods and results: spoilage bacteria isolated from pasteurized milk at 8 and 15°c were identified as enterococcus italicus, enterococcus mundtii, enterococcus faecalis, bacillus thuringiensis, bacillus cereus, lactobacillus paracasei, acinetobacter sp., pseudomonas fluorescens and enterobacter a ... | 2011 | 21554339 |
| cytotoxic and antimicrobial activity of pseudopterosins and seco-pseudopterosins isolated from the octocoral pseudopterogorgia elisabethae of san andrés and providencia islands (southwest caribbean sea). | to expand the potential of pseudopterosins and seco-pseudopterosins isolated from the octocoral pseudopterogorgia elisabethae of san andrés and providencia islands (southwest caribbean sea), we report the anti-microbial profile against four pathogenic microorganisms (staphylococcus aureus, enterococcus faecalis, pseudomonas aeruginosa and candida albicans) and report a more complete cytotoxic profile against five human cells lines (hela, pc-3, hct116, mcf-7 and bj) for the compounds psg, psp, ps ... | 2011 | 21556163 |
| in vitro antimicrobial activity of propolis, biopure mtad, sodium hypochlorite, and chlorhexidine on enterococcus faecalis and candida albicans. | to evaluate the antimicrobial effect by measuring the minimum inhibitory concentration mic and minimum bactericidal concentration mbc of propolis, biopure mtad, 5% sodium hypochlorite (naocl), and 2% chlorhexidine chx on enterococcus faecalis (e. faecalis) and candida albicans (c. albicans) in vitro. | 2011 | 21556468 |
| intra- and interspecies genomic transfer of the enterococcus faecalis pathogenicity island. | enterococci are the third leading cause of hospital associated infections and have gained increased importance due to their fast adaptation to the clinical environment by acquisition of antibiotic resistance and pathogenicity traits. enterococcus faecalis harbours a pathogenicity island (pai) of 153 kb containing several virulence factors including the enterococcal surface protein (esp). until now only internal fragments of the pai or larger chromosomal regions containing it have been transfered ... | 2011 | 21559082 |
| increased risk of vancomycin-resistant enterococcus (vre) infection among patients hospitalized for inflammatory bowel disease in the united states. | vancomycin-resistant enterococcus (vre) infection has become an increasingly common hospital-acquired infection in u.s. hospitals. patients with inflammatory bowel disease (ibd) frequently require hospitalization and therefore may be at increased risk of nosocomial infections. | 2011 | 21560197 |
| monitoring of water quality from roof runoff: interpretation using multivariate analysis. | the quality of harvested rainwater used for toilet flushing in a private house in the south-west of france was assessed over a one-year period. temperature, ph, conductivity, colour, turbidity, anions, cations, alkalinity, total hardness and total organic carbon were screened using standard analytical techniques. total flora at 22 °c and 36 °c, total coliforms, escherichia coli and enterococci were analysed. overall, the collected rainwater had good physicochemical quality but did not meet the r ... | 2011 | 21561638 |
| crystal structures of staphylococcus epidermidis mevalonate diphosphate decarboxylase bound to inhibitory analogs reveal new insight into substrate binding and catalysis. | the polyisoprenoid compound undecaprenyl phosphate is required for biosynthesis of cell wall peptidoglycans in gram-positive bacteria, including pathogenic enterococcus, streptococcus, and staphylococcus. in these organisms, the mevalonate pathway is used to produce the precursor isoprenoid, isopentenyl disphosphate (ipp). mevalonate diphosphate decarboxylase (mdd) catalyzes formation of ipp in an atp dependent, irreversible reaction, and is therefore an attractive target for inhibitor developme ... | 2011 | 21561869 |
| secondary transfer and expression of vana in enterococcus faecium derived from a commensal vancomycin-susceptible enterococcus faecium multi-component food isolate. | we investigated the potential for vancomycin-susceptible enterococcus (vse) from multi-component salads to disseminate vana from four clinical vancomycin-resistant enterococci to 14 streptogramin-resistant enterococci (sre) of food and animal origin. strains were selected from a previous study based on the presence of streptogramin susceptibility and/or vana, vanb, vatd, vate, agg, cpd, and gele genes. transconjugants were isolated on brain heart infusion agar containing vancomycin and selective ... | 2011 | 21563943 |
| genetic detection and multilocus sequence typing of vana-containing enterococcus strains from mullets fish (liza ramada). | enterococci have emerged as important nosocomial and community-acquired pathogens in humans. the presence of vana-enterococci was investigated in 103 fecal samples recovered from mullets fish (liza ramada). all fecal samples were inoculated in slanetz-bartley agar plates supplemented with 4 mg/l of vancomycin for vancomycin-resistant enterococci (vre) recovery and two isolates/sample were characterized. antibiotic susceptibility was tested for 11 antibiotics by disk diffusion and agar dilution m ... | 2011 | 21563953 |
| detection of the macrolide-efflux protein a gene mef(a) in enterococcus faecalis. | the mef(a) gene codes for an efflux protein that conveys resistance to 14- and 15-membered macrolides. enterococci are emerging pathogens, as well as indicator and reservoir bacteria that are known to have a strong tendency to acquire resistance genes. a total of 485 enterococcus faecalis strains of porcine (n = 239) and human origin (n = 246) were screened for the presence of the mef(a) gene by using polymerase chain reaction. in total, 29 e. faecalis of porcine (n = 10) and human (n = 19) orig ... | 2011 | 21563955 |
| [trends of causative and antimicrobial resistant organisms among patients with hematological malignancies in japan]. | 2011 | 21566402 | |
| enterococcus populations in artisanal manchego cheese: biodiversity, technological and safety aspects. | enterococci represent a considerable proportion of the microbiota in manchego cheeses. in this study, a total of 132 enterococci isolated from good quality manchego cheeses from two dairies at different ripening times were genotypically characterized and identified using molecular techniques. representative isolates from the clusters obtained after genotyping were assayed for some enzymatic activities considered to have a potential role in cheese ripening, and for 2,3-butanedione and acetoin pro ... | 2011 | 21569931 |
| microbiological characteristics of kumis, a traditional fermented colombian milk, with particular emphasis on enterococci population. | kumis is a traditional fermented cow milk produced and consumed in south west colombia. the main objective of this research was to studied the enterococcal population, present in 13 kumis samples traditionally manufactured, for their role as beneficial organisms or opportunistic pathogens. the molecular identification of 72 isolates evidenced that enterococcus faecalis and e. faecium were the dominant species. the genes gele, esp, asa1, cyl and hyl, all associated with virulence factors in enter ... | 2011 | 21569950 |
| evaluation of microbiological water quality in the pettaquamscutt river (rhode island, usa) using chemical, molecular and culture-dependent methods. | we evaluated microbiological water quality in the pettaquamscutt river (rhode island, usa), an estuarine river. fecal coliform (fc) and enterococci (fe) bacteria, presence of bifidobacterium adolescentis dna (indicating human fecal contamination), and optical brightener (ob) fluorescence (associated with laundry detergents) were determined for 14 stations from may to september 2010. six stations had high counts of fe and fc, and the presence of b. adolescentis dna and high ob fluorescence indica ... | 2011 | 21570698 |
| eight-year (2002-2009) summary of the linezolid (zyvox(r) annual appraisal of potency and spectrum; zaaps) program in european countries. | the linezolid surveillance network (zaaps program) has been monitoring linezolid activity and susceptibility rates for eight years (2002-2009) in european medical centers. samples from 12-24 sites annually in 11 countries were monitored by a central laboratory design using reference mic methods with international and regional interpretations (eucast). a total of 13,404 gram-positive pathogens were tested from 6 pathogen groups. linezolid remained without documented resistance from 2002 through 2 ... | 2011 | 21571621 |
| characterization of the mechanisms of fluoroquinolone resistance in vancomycin-resistant enterococci of different origins. | the mutations in gyra and parc genes were analyzed in 22 vancomycin-resistant enterococci of different origins and species, which had varying susceptibility to ciprofloxacin (minimum inhibitory concentration, mic: 0.5- >256 mg/l). all vana or vanb2-containing strains with ciprofloxacin mic of >32 mg/l presented amino acid changes in gyra protein (s83i, s83y, s83r or s83i-e87g) with/without changes in parc protein (s80i or s80r or s80l). strains with lower ciprofloxacin mics presented the gyra an ... | 2011 | 21571624 |
| new noncovalent inhibitors of penicillin-binding proteins from penicillin-resistant bacteria. | penicillin-binding proteins (pbps) are well known and validated targets for antibacterial therapy. the most important clinically used inhibitors of pbps β-lactams inhibit transpeptidase activity of pbps by forming a covalent penicilloyl-enzyme complex that blocks the normal transpeptidation reaction; this finally results in bacterial death. in some resistant bacteria the resistance is acquired by active-site distortion of pbps, which lowers their acylation efficiency for β-lactams. to address th ... | 2011 | 21573060 |
| efficacy of antibacterial sealing gel and o-ring to prevent micro leakage at the implant abutment interface - an invitro study. | abstract gaps and hollow spaces at the implant abutment interface will act as a bacterial reservoir which may cause peri implantitis . hence, the sealing ability of o-ring [an addition polysiloxane] and gapseal[ an antibacterial sealing gel] was evaluated .a total of 45 identical implant systems of adin company were taken and divided into 3 groups with 15 implants in each group. the group labeled u consisted of unsealed implants; the group o consisted of implants sealed with o-rings and the grou ... | 2011 | 21574837 |
| characterization and validation of a chemiluminescent assay based on a 1,2-dioxetane for rapid detection of enterococci in contaminated water and comparison with standard methods and qpcr. | aims: to evaluate the potential for using a novel chemiluminescence-based enzyme assay for rapid detection of enterococci in water contaminated with faecal waste. methods and results: the novel assay (entlight) was based on the enzymatic hydrolysis of the chemiluminescent 1,2-dioxetane [(4-methoxy-4(3-β-d-glucoside-4-chlorophenyl)]spiro[1,2-dioxetane-3-1,3-tricyclo[7·3·1·0(2,7) ]tridec-2,7-ene] specific for β-d-glucosidase. the specificity of the proposed entlight assay was characterized using ... | 2011 | 21575110 |
| [antibiotic resistance of pathogens isolated from 181 children with complicated urinary tract infection.] | objective: to investigate the distribution and antibiotic resistance of pathogens isolated from children with complicated urinary tract infection. methods: a retrospective analysis was performed on the distribution and antibiotic resistance of pathogens isolated from 181 children with complicated urinary tract infection (positive urine culture). the antibiotic resistance of common pathogens was determined by the antimicrobial susceptibility test. results: gram-negative bacilli were the main path ... | 2011 | 21575342 |
| antimicrobial properties of mx-2401, a second generation lipopeptide active in presence of lung surfactant. | mx-2401 is a second generation gram-positive-selective lipopeptide antibiotic that is a semi-synthetic analog of the naturally occurring lipopeptide amphomycin. it was active against enterococcus spp. including vancomycin-sensitive (vse), vana, vanb, and vanc positive vancomycin-resistant (vre), linezolid- and quinupristin/dalfopristin-resistant isolates (mic(90) of 4 μg/ml), methicillin-resistant (mrsa) and methicillin-sensitive (mssa) staphylococcus aureus (mic(90) of 2 μg/ml), coagulase-negat ... | 2011 | 21576435 |
| analysis of pbp5 of early united states enterococcus faecium isolates: sequence variation alone does not explain increasing ampicillin resistance over time. | recent studies have shown that ampicillin resistance has increased steadily over the past three decades within united states enterococcus faecium isolates. analysis of the predicted pbp5 protein of 41 isolates showed a consensus pbp5 pattern for the 9 isolates with mics < 4μg/ml that is distinctly different from the pbp5 consensus of the 32 isolates with mics > 4μg/ml with ∼5% difference between these; however, there were no consistent amino acid changes that correlated with specific increases i ... | 2011 | 21576454 |
| the microbiology of secondary and postoperative pancreatic infections: implications for antimicrobial management. | we reviewed our experience with secondary pancreatic infections with a focus on preemptive intervention and the potential alteration of the recovered microbial flora. the pathogens associated with postoperative pancreatic infections were analyzed with respect to nonenteric organisms, if any, that were recovered. we hypothesized that our findings might alter the antimicrobial management of these patients. | 2011 | 21576614 |
| susceptibility of gram-positive and -negative bacteria to novel nitric oxide-releasing nanoparticle technology. | the rapidly evolving crisis of antibiotic resistance among microorganisms has contributed to the rise of patient morbidity and mortality from nosocomial and community-acquired infections. therefore, innovative antimicrobial technology targeting mechanisms to which the bacteria are unlikely to evolve resistance is urgently needed. we have previously described a nitric oxide-releasing nanoparticle (no-np) with efficacy against methicillin-resistant staphylococcus aureus (mrsa) and acinetobacter ba ... | 2011 | 21577055 |
| enterococcus faecalis affects the proliferation and differentiation of ovine osteoblast-like cells. | enterococcus faecalis (e. faecalis) is a gram-positive bacterium, mostly recovered from root-filled teeth with persistent periapical lesions. bacterial contamination of root canals inevitably results in interaction between e. faecalis and periapical tissues during the dynamic process of periapical inflammation. this study investigated the impact of heat-inactivated endodontic e. faecalis on the proliferation and the differentiation of ovine osteoblast-like cells, in an attempt to elucidate its p ... | 2011 | 21584693 |
| from commensal to pathogen: translocation of enterococcus faecalis from the midgut to the hemocoel of manduca sexta. | abstract a dynamic homeostasis is maintained between the host and native bacteria of the gastrointestinal tract in animals, but migration of bacteria from the gut to other organs can lead to disease or death. enterococcus faecalis is a commensal of the gastrointestinal tract; however, enterococcus spp. are increasingly frequent causes of nosocomial infections with a high mortality rate. we investigated the commensal-to-pathogen switch undergone by e. faecalis og1rf in the lepidopteran model host ... | 2011 | 21586646 |
| as-48 bacteriocin: close to perfection. | bacteriocin as-48 is an intriguing molecule because of its unique structural characteristics, genetic regulation, broad activity spectrum, and potential biotechnological applications. it was the first reported circular bacteriocin and has been undoubtedly the best characterized for the last 25 years. thus, as-48 is the prototype of circular bacteriocins (class iv), for which the structure and genetic regulation have been elucidated. this review discusses the state-of-the-art in genetic engineeri ... | 2011 | 21590312 |
| structure-based design of a new series of d-glutamic acid based inhibitors of bacterial udp-n-acetylmuramoyl-l-alanine:d-glutamate ligase (murd). | murd ligase is one of the key enzymes participating in the intracellular steps of peptidoglycan biosynthesis and constitutes a viable target in the search for novel antibacterial drugs to combat bacterial drug-resistance. we have designed, synthesized, and evaluated a new series of d-glutamic acid-based escherichia coli murd inhibitors incorporating the 5-benzylidenethiazolidin-4-one scaffold. the crystal structure of 16 in the murd active site has provided a good starting point for the design o ... | 2011 | 21591605 |
| variation in antibiotic-induced microbial recolonization impacts on the host metabolic phenotypes of rats. | the interaction between the gut microbiota and their mammalian host is known to have far-reaching consequences with respect to metabolism and health. we investigated the effects of eight days of oral antibiotic exposure (penicillin and streptomycin sulfate) on gut microbial composition and host metabolic phenotype in male han-wistar rats (n = 6) compared to matched controls. early recolonization was assessed in a third group exposed to antibiotics for four days followed by four days recovery (n ... | 2011 | 21591676 |
| mycobacterium behavior in wastewater treatment plant, a bacterial model distinct from escherichia coli and enterococci. | mycobacteria are waterborne emerging pathogens causing infections in human. mycobacteria have been previously isolated from wastewater and sludge, but their densities were not estimated due to cultural biases. in order to evaluate the impact of wastewater treatment processes on mycobacteria removal, we used a real time pcr method. first we compared six dna extraction methods and second we used the more efficient dna extraction procedure (i.e., enzymatic lysis combined with hexadecyltrimethylammo ... | 2011 | 21591688 |
| oral administration of multispecies microbial supplements to sows influences the composition of gut microbiota and fecal organic acids in their post-weaned piglets. | the timings of the administration of microbial supplements to control the populations of gut microbiota of piglets have been poorly understood. here the effects of temporal administering multispecies microbial supplements to sows on the composition of gut microbiota and on the bacteria-mediated fecal metabolites in their offsprings were investigated. during gestation and lactation, pregnant sows were fed either a normal diet (group a) or a diet with multispecies supplements comprised of nine mic ... | 2011 | 21592857 |