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active transport of na + and k + through animal cell membranes. 20144336410
[knee symptoms in children]. 20143974742
[fracture of the coracoid process]. 20144261574
[two- and three dimensional ultrasonic technics in medical diagnosis]. 20144895631
regional migratory osteoporosis.the authors describe the features of regional migratory osteoporosis, a rare form of idiopathic algodystrophy, with special reference to the fact that 8 of the 13 patients in this series belonged to two families. the clinical, radiological and histological manifestations are discussed and the treatment described. the latter involves a combination of chemotherapy, orthopaedic measures and physiotherapy. the fact that so many different clinical manifestations of the same pathological entity occurr ...20144086283
[the dysplastic hip joint. a report of 4 cases (author's transl)]. 20144277292
[unverricht-lundborg myoclonic epilepsy in 3 siblings]. 20144252636
[problems posed by compensation of sequelae of poliomyelitis within the framework of the law concerning military pensions]. 20144262381
permanent cardiac pacemakers in alaska natives with complete heart block. 20144247686
measurement of cavitation activity in ultrasonic cleaners. 20144227629
correlation of electroencephalographic, reaction time and auditory thresholds on 24 hour recording. 20144187381
clinical significance of rheumatoid vasculitis and the incidence of digital vascular lesions. 20144313127
audiovisual education in dermatology. 20144132225
on the alleged ineffability of death.a number of recent theorists, all proceeding on the assumption that there is no afterlife, argue that the notion of one's own death defies comprehension in ways that have important implications. some argue this "ineffability" of death precludes a rational decision to die. dr. edwin shneidman maintains that it makes the notion of our own death unintelligible to each of us. in this paper, these arguments are criticized. it is maintained that death is not so inscrutible as these theorists would hav ...20144089936
australia antigen. 20144143602
[electromyographic study of 250 cases of surgically treated lumbar radicular syndromes]. 20144418640
cefamandole levels in serum and necrotic bone.seven patients with chronic osteomyelitis were treated by surgical debridement. cefamandole was administered intravenously before surgery. during the debridement, cefamandole concentrations were measured in serum and necrotic bone. although adequate levels of antibiotic were achieved in the serum, minimal or no concentration of antibiotic was found in the necrotic bone. there was only minimal penetration of cefamandole into necrotic bone.20144042490
[diagnostic difficulties in subdural hematoma]. 20143993034
effect of postpartum lactation on lipoprotein lipids and apoproteins.to determine if postpartum lactation alters plasma lipoprotein lipid and apoprotein concentrations and composition, we studied 56 overnight fasting lactating and 16 nonlactating women approximately 6 weeks postpartum. postpartum results are presented as absolute concentrations and as the difference from antepartum values determined at 36 weeks gestation. antepartum lipoprotein lipid and apoprotein concentrations were generally not different in the 2 groups, with the single exception of whole pla ...20143972965
radioenzymatic assay for trimethoprim in very small serum samples.a modification of the methotrexate radioassay kit (supplied by new england enzyme center) enabled determination of trimethoprim levels in 5-microliter serum samples. an excellent correlation between this assay and high-pressure liquid chromatography assay was found. these preliminary results suggest that with this method rapid determination of trimethoprim levels in very small samples (5 to 10 microliters) can be achieved.20143972993
[air pollution]. 20144858019
restoration of missing or misplaced canines.restorative treatments for canines were discussed to correct three clinical abnormalities: (1) fully erupted permanent canine in the lateral incisor position, (2) missing permanent canines, and (3) partially exposed canines in normal arch position. the primary concerns are the development of esthetics, anterior guidance, and adequate support for fixed restorations.20143891977
dental hygiene's new initiative--how can we work together? reaction paper. 20143866858
entangled urinary catheters--prevention of a "knotty" problem. 20144063747
drugs and dreams. iv. tranquilizers and their effects upon dreams and dreaming in schizophrenic patients. 20144313272
[changes in some biochemical indices in workers exposed to the risk of lead poisoning]. 20144187855
[modes of case-finding of tuberculous patients in rural areas]. 20144042781
personal view: in-hospital patient management strategies after acute myocardial infarction.the coronary patient after the striking experience of a myocardial infarction faces a completely new situation possibly affecting lifestyle, occupation and life expectancy. a dedicated post-infarction diagnostic strategy for the evaluation of the individual risk of suffering from a future coronary event, or of dying, requires profound knowledge of the impact of medical and surgical therapies on the survival rate and on the quality of life after infarction.20144054133
omental protection of autogenous arterial reconstruction following femoral prosthetic graft infection.vascular graft infections in the femoral region that require synchronous revascularization are generally reconstructed with prosthetic grafts via extra-anatomic routes. if in situ revascularization is required, then autologous tissue provides optimal results. a particular challenge in this circumstance is achieving soft tissue coverage of the reconstruction where wide and radical débridement has removed the tissues ordinarily used for this purpose. if muscle flaps are not available or possible, ...20144009844
[microsurgery in gynecology--indications, results and limitations]. 20133616963
preventive dentistry update--dental sealants. 20133590771
[clear announcement from nurses: wages must go up]. 20133644577
gonadotropin releasing hormone elicits abnormal hormone responses in schizophrenia.we studied the non-specific responses of gh and prl to gonadotropin releasing hormone (gnrh) in eleven male patients aged 18-30 in whom a diagnosis of acute schizophrenia was made according to crow's criteria. gnrh administration was followed by a significant increase in plasma gh in five patients; plasma prl increased in two patients. the two prolactin responders were also gh responders. non-specific gh response was confirmed on repeated testing in two patients in whom gnrh stimulation was perf ...20133911248
gonadotropin releasing hormone elicits abnormal hormone responses in schizophrenia.we studied the non-specific responses of gh and prl to gonadotropin releasing hormone (gnrh) in eleven male patients aged 18-30 in whom a diagnosis of acute schizophrenia was made according to crow's criteria. gnrh administration was followed by a significant increase in plasma gh in five patients; plasma prl increased in two patients. the two prolactin responders were also gh responders. non-specific gh response was confirmed on repeated testing in two patients in whom gnrh stimulation was perf ...20133911248
electrophysiologic study in supraventricular arrhythmias. 20133842327
[the importance of group conferences for team nursing]. 20134494435
[dioxins--a health hazard?]. 20133726834
[intestinal parasitoses].while intestinal parasites are infrequent causes of acute diarrhea, investigations in travellers, children, homosexuals and immunocompromised persons who have had diarrhea for more than 2-4 weeks should include stool analyses for intestinal parasites. intestinal helminthiases are treated with pyrantel pamoate, mebendazole, thiabendazole, albendazole, praziquantel or niclosamide. for treatment of intestinal protozoa, ornidazole (or other benzimidazoles), diloxanide furoate and co-trimoxazole are ...20133726513
two-hour versus 22-hour creatinine clearance in critically ill patients. 20133688525
monomer-oligomer equilibrium of bacteriorhodopsin in reconstituted proteoliposomes. a freeze-fracture electron microscope study.an improved freeze-fracture electron microscope procedure has been developed and applied to the study of the association of bacteriorhodopsin in large proteoliposomes reconstituted by reverse-phase evaporation with egg lecithin. due to the improved accuracy and resolution of this procedure, intramembrane particles, the diameter of which (4.5 nm) closely matched that of bacteriorhodopsin monomer, could be observed at high lipid to protein ratios (greater than or equal to 40 w/w). at lower lipid t ...20133680213
[the patients who accidentally learned the nature of their illnesses]. 20133640964
nursing in the 21st century. proceedings of the american association of colleges of nursing (aacn) and the american organization of nurse executives (aone) conference, aspen, colorado july 9-11, 1985. 20133639099
[a thought on family education for smoother return of apoplexy patients to home]. 20133637287
assessing childhood depression: do parents and children agree? 20133633069
ultrastructure and changes during metamorphosis of the lympho-hemopoietic tissue of the larval anadromous sea lamprey petromyzon marinus.the ultrastructure of the lympho-hemopoietic tissue of the typhlosole and opisthonephros of larval petromyzon marinus was studied throughout the life cycle. in both locations, lympho-hemopoietic tissue houses a connective tissue stroma and is irrigated by a system of enlarged blood sinuses lined by a continuous endothelium. the typhlosolar and opisthonephric lympho-hemopoietic tissue consists of mature and developing blood cells belonging to erythroid, lymphoid, monocytic and granulocytic lineag ...20133595947
an approach to client satisfaction measurement as an attribute of health service quality.client satisfaction plays an important role in the assessment of health services quality used to establish institutional loyalty, usage patterns, word-of-mouth communications, and presumably to enhance the rate by which clients attain satisfactory levels of health. the makeup of attitude, its measurement, and a methodology for the production of a relevant health service-specific client satisfaction survey are presented.20133623908
[primary mesangiocapillary glomerulonephritis]. 20133599858
solvent effects on reversible formation and oxidative stability of heme-oxygen complexes. 20134854321
coliphage p2 late control gene ogr. dna sequence and product identification.the bacteriophage p2 late control gene ogr was cloned and precisely localized by deletion analysis in vitro. the dna sequence of the ogr gene containing the ogr1 mutation was determined. the sequence translates into a basic protein of a molecular weight of 8300. plasmids overproducing the ogr gene product were constructed, and the ogr gene product was identified by polyacrylamide gel electrophoresis.20133735430
changing role of the veterinary nurse. 20133716091
[pre and postoperative care of aged patients with metabolic or endocrine disorders]. 20133637325
reduction of intraocular pressure with dexamethasone. 20134105071
mediation of cellular events by thrombin. 20133536791
[magnesium and calcium metabolism in peptic ulcer patients]. 20133798889
ruptured abdominal aortic aneurysm in a 24-year-old african woman. unusual presentation--unknown aetiology.24-year-old black female presenting with progressive paralysis of the left lower limb. the clinical examination and investigations describe an extensive retroperitoneal mass. an exploratory laparotomy reveals a ruptured saccular infrarenal aortic aneurysm, a narrowing of the distal aorta and a complete obstruction of the left common iliac artery. the aneurysm was resected, an aortoiliac bypass and femorofemoral crossover bypass were performed, with success. the clinical presentation of this rupt ...20133766024
parental anxiety related to the care of a child with apnea. 20133638291
[comparative study on the bioavailability of progesterone by radioimmunoassay after vaginal and intramuscular administration]. 20133591325
smoking behavior and attitudes toward smoking among hospital nurses.we examined smoking prevalence, smoking behavior, and attitudes toward smoking in hospitals in 1,380 respondents among 1,719 registered nurses in a large urban teaching hospital. in this group, current prevalence of smoking in hospital nurses (22 per cent) was less than women in the general population (29 per cent). smoking nurses were more likely than nonsmokers to hold attitudes which potentially reduce their efficacy in helping patients to stop smoking.20133777295
[surgical treatment of pain]. 20133719653
self-poisoning: outcome and complications in the community hospital.one hundred sixty cases of self-poisoning of patients aged 15 years and older were treated on the wards of the four community hospitals of lansing, michigan, in 1981. most cases (91 percent) were intentional and represented suicide attempts. the complication rate was 13.8 percent and the overdose-related mortality rate 0.6 percent. aspiration pneumonia was the most common complication, followed by respiratory failure and seizures. no patient with a level of consciousness stage 0 or 1 in the emer ...20133746211
[rhabdomyosarcoma in childhood. ii. ultrastructure].the diagnosis of rhabdomyosarcoma was established or proved by electron microscopy in two thirds of 21 cases. histological classification was not adequate or formulated as "undifferentiated sarcoma" in half the cases. electron microscopy did not succeed in one third of cases and rhabdomyosarcoma was classified according to simultaneous or successive study. undifferentiated tumour cells resembled myoblastema cells of the 5th-6th gestation weeks. differentiation of myoblasts and myotubes correspon ...20134064135
[rhabdomyosarcoma in childhood. ii. ultrastructure].the diagnosis of rhabdomyosarcoma was established or proved by electron microscopy in two thirds of 21 cases. histological classification was not adequate or formulated as "undifferentiated sarcoma" in half the cases. electron microscopy did not succeed in one third of cases and rhabdomyosarcoma was classified according to simultaneous or successive study. undifferentiated tumour cells resembled myoblastema cells of the 5th-6th gestation weeks. differentiation of myoblasts and myotubes correspon ...20134064135
intracranial hypertension in behçet's disease: demonstration of sinus occlusion with use of digital subtraction angiography.we describe two patients with behçet's disease who had symptomatic intracranial hypertension due to cerebral venous sinus thrombosis. the sinus thrombosis was demonstrated by digital subtraction angiography. in those patients with behçet's disease who have unexplained headaches, papilledema, and elevated cerebrospinal fluid pressure, venous digital subtraction angiography is an expedient, accurate, and safe procedure for demonstrating intracranial venous thrombosis.20133889512
[treatment of mushroom poisoning]. 20134590880
hospital design follows the crowd to ambulatory care. 20133804279
[how can the norwegian nurses' association use professional resources better?]. 20133649971
paracetamol and phenacetin.since their synthesis in the late 1800s paracetamol (acetaminophen) and phenacetin have followed divergent pathways with regard to their popularity as mild analgesic/antipyretic drugs. initially, paracetamol was discarded in favour of phenacetin because the latter drug was supposedly less toxic. today the opposite is true, and paracetamol, along with aspirin, has become one of the two most popular 'over-the-counter' non-narcotic analgesic agents. this marked increase in the wide approval attaine ...20133552585
immunocytochemical markers of uncommon pancreatic tumors. acinar cell carcinoma, pancreatoblastoma, and solid cystic (papillary-cystic) tumor.nine acinar cell carcinomas of the pancreas, 2 pancreatoblastomas, 16 solid-cystic (papillary-cystic) tumors, and 20 ductal adenocarcinomas were immunocytochemically investigated using antisera against four pancreatic enzymes (alpha-amylase, lipase, trypsinogen, chymotrypsinogen), four pancreatic hormones, neuron specific enolase (nse), alpha-1-antitrypsin (aat), carcinoembryonic antigen (cea), and ca 19-9. lipase, trypsinogen, and chymotrypsinogen, but no alpha-amylase were detected in all acin ...20133542187
a non-computer expert's experience with the computer.in summary, i have tried to illustrate how the computer is rapidly becoming indispensable to the physician. this is true for both the physician in private practice as well as the physician involved in research. computer technology has progressed to the point that a personal computer is readily affordable. technologic advances and new software packages permit individuals who possess no computer skills to operate almost any system without limitations. there are no age limits to learn how to use th ...20133536224
the role of cyclic nucleic acid adducts in carcinogenesis and mutagenesis. proceedings of a meeting. lyon, 17-19 september 1984. 20133793166
molecular detection and characterization of tick-borne pathogens in dogs and ticks from nigeria.only limited information is currently available on the prevalence of vector borne and zoonotic pathogens in dogs and ticks in nigeria. the aim of this study was to use molecular techniques to detect and characterize vector borne pathogens in dogs and ticks from nigeria.201323505591
molecular detection of ehrlichia canis in dogs in malaysia.an epidemiological study of ehrlichia canis infection in dogs in peninsular malaysia was carried out using molecular detection techniques. a total of 500 canine blood samples were collected from veterinary clinics and dog shelters. molecular screening by polymerase chain reaction (pcr) was performed using genus-specific primers followed by pcr using e. canis species-specific primers. ten out of 500 dogs were positive for e. canis. a phylogenetic analysis of the e. canis malaysia strain showed th ...201323301114
frequency and clinical epidemiology of canine monocytic ehrlichiosis in dogs infested with ticks from sinaloa, mexico.ehrlichia canis is a rickettsial intracellular obligate bacterial pathogen and agent of canine monocytic ehrlichiosis. the prevalence of this disease in veterinary medicine can vary depending on the diagnostic method used and the geographic location. one hundred and fifty-two canine blood samples from six veterinary clinics and two shelters from sinaloa state (mexico) were analyzed in this study. all animals were suspected of having canine monocytic ehrlichiosis (cme). the diagnostic methods use ...201326464910
seropositivity rates for agents of canine vector-borne diseases in spain: a multicentre study.controlling canine vector-borne diseases (cvbd) is a major concern, since some of these diseases are serious zoonoses. this study was designed to determine seropositivity rates in spain for agents causing the following five cvbd: leishmaniosis (leishmania infantum: li), heartworm (dirofilaria immitis: di), ehrlichiosis (ehrlichia canis: ec), anaplasmosis (anaplasma phagocytophilum/anaplasma platys: an) and lyme disease (borrelia burgdorferi: bb).201323607428
isolation, in vitro propagation, genetic analysis, and immunogenic characterization of an ehrlichia canis strain from southeastern brazil.amplification of the 16s rrna gene from a blood sample obtained from a dog in southeastern brazil was used to confirm a naturally acquired ehrlichia (e.) canis infection. following isolation and culturing of the new bacterial strain called uberlandia, partial sequences of the dsb and p28 genes were obtained. the dsb partial sequence of the novel strain was 100% similar to dsb gene sequences of e. canis obtained from different geographic areas around the world. conversely, the p28 partial sequenc ...201324136211
serosurvey of tick-borne pathogens in dogs from urban and rural areas from parana state, brazil.considering the zoonotic potential of tick-borne disease (tbd) agents and the fact that dogs may act as sentinels for human infection, the aim of the present study was to determine the seroprevalence of tbd agents and risk factors for exposure in two different canine populations from parana state, southern brazil. a total of 138 dog serum samples from urban (ua) (n=68) and rural (ra) (n=70) areas were tested with commercial elisa rapid test for anaplasma phagocytophilum, ehrlichia canis and borr ...201324252955
prevalence of borreliosis, anaplasmosis, ehrlichiosis and dirofilaria immitis in dogs and vectors in voronezh reserve (russia).most of the dogs studied for the prevalence of cvbd have previously received acaricidal and insecticidal treatments. in the present work, a very specific population of dogs (group 1) that had never been treated against ticks and mosquitoes was studied. moreover, the territory occupied by this population has also never been treated, because it is a protected area--voronezh natural reserve. canine patients from veterinary clinics (group 2) that had been treated against vbd vectors were studied for ...201324054985
vector-borne disease surveillance in puerto rico: pathogen prevalence rates in canines ? implications for public health and the u.s. military ? applying the one health concept.vector-borne diseases (vbds) make up a large number of emerging infectious and zoonotic diseases. vectors such as ticks, fleas, and mosquitoes parasitize dogs, thus making canine populations adequate reservoirs for infectious disease and zoonoses. the u.s. military deploys its personnel and military working dogs (mwds) throughout the world with possible risk of exposure to vbds. canine vbds continue to have veterinary and public health significance for the host nations as well as for deployed u. ...201323817880
prevalence of antibodies to spotted fever group rickettsia spp. and ehrlichia spp. in coyotes (canis latrans) in oklahoma and texas, usa.coyotes (canis latrans) are commonly infested with ticks, including amblyomma americanum, the predominant vector of ehrlichia chaffeensis and ehrlichia ewingii; dermacentor variabilis, an important vector of rickettsia rickettsii; and amblyomma maculatum, a major vector of rickettsia parkeri, a spotted fever group (sfg) rickettsia. to determine the degree to which coyotes are infected with or exposed to tick-borne bacterial disease agents, serum samples collected from coyotes in oklahoma and tex ...201323778619
molecular evidence for bacterial and protozoan pathogens in hard ticks from romania.the aim of the present study was to provide a preliminary insight into the diversity of tick-borne pathogens circulating at the domestic host-tick interface in romania. for this, feeding and questing ticks were analyzed by real-time polymerase chain reaction (pcr) for the presence of anaplasma phagocytophilum, anaplasma platys, ehrlichia canis, borrelia burgdorferi sensu latu, and by pcr and subsequent sequencing for rickettsia spp., babesia spp. and theileria spp. a total of 382 ticks, encompas ...201323428204
the efficacy of advantix® to prevent transmission of ehrlichia canis to dogs by rhipicephalus sanguineus ticks.the capacity of a topical combination of imidacloprid and permethrin (advantix(®)) to prevent transmission of ehrlichia canis was studied in two groups of six dogs. one group served as controls, whereas the other group was treated. all dogs were exposed to e. canis-infected rhipicephalus sanguineus ticks on days 7, 14, 21 and day 28 post acaricidal treatment. the adult r. sanguineus ticks were released into the individual kennels of the dogs to simulate natural tick exposure. in situ tick counts ...201324135158
molecular epidemiologic survey of bartonella, ehrlichia, and anaplasma infections in japanese iriomote and tsushima leopard cats.the iriomote cat (ic; prionailurus iriomotensis) and the tsushima leopard cat (tlc; prionailurus bengalensis euptilura) are endangered wild felids in japan. as a part of ongoing conservation activities, we conducted a molecular, epidemiologic survey of bartonella, ehrlichia, and anaplasma infections in wild ic and tlc populations. blood samples (47 from 33 individual ic; 22 from 13 tlc) were collected between august 2002 and january 2011. using pcr analysis, we confirmed the presence of bartonel ...201323778615
a molecular survey of anaplasma spp., rickettsia spp., ehrlichia canis and babesia microti in foxes and fleas from sicily.fleas (insecta: siphonaptera) are obligate bloodsucking insects, which parasitize birds and mammals, and are distributed throughout the world. several species have been implicated in pathogen transmission. this study aimed to monitor red foxes and the fleas isolated from them in the palermo and ragusa provinces of sicily, italy, as these organisms are potential reservoirs and vectors of pathogens. thirteen foxes (vulpes vulpes) and 110 fleas were analysed by polymerase chain reaction (pcr) to de ...201324589112
molecular detection of ehrlichia canis and anaplasma platys in dogs in southern brazil.the aims of this study were to determine the occurrence of anaplasma platys and ehrlichia canis infection in dogs in porto alegre, southern brazil; and to investigate their association with hematological abnormalities. serum samples from 196 dogs were first tested using dot-elisa for antibodies against anaplasma spp. and ehrlichia canis. peripheral blood samples from 199 dogs were subjected to 16s rrna nested pcr (npcr) for a. platys and e. canis, followed by dna sequencing to ensure pathogen id ...201324142166
babesia canis vogeli, ehrlichia canis, and anaplasma platys infection in a dog.a 12-month-old male neutered mixed breed dog was presented with a history of diarrhea, lethargy, emaciation, polydypsia, and sniffling. physical examination findings included pale mucous membranes, increased heart and respiratory rates, and normal rectal temperature (38°c). hematologic abnormalities included anemia and thrombocytopenia. biochemical abnormalities included hypoalbuminemia, hyperbilirubinemia, and elevated alp and alt activities. a snap 4dx test result was positive for ehrlichia ca ...201324138512
detection of ehrlichia canis and anaplasma platys dna using multiplex pcr.we hereby propose a novel sensitive, specific, and cost-efficient method to detect ehrlichia canis and anaplasma platys dna from canine whole blood samples by multiplex pcr. blood samples from hemoparasited dogs attending the veterinary hospital at the universidade federal rural da amazônia-ufra, belém, brazil, were collected in tubes containing edta. amplification of e. canis and a. platys 16s rdna by nested (n) pcr was successfully achieved by using primers specific to the anaplasmataceae in t ...201324107211
study of ehrlichiosis in kennel dogs under treatment and prevention during seven months in dakar (senegal).in dakar kennels where morbidity and mortality attributed to diseases transmitted by ticks were high, we conducted a field study to assess the prevalence of ehrlichia canis, anaplasma platys and babesia spp. infections in two kennels (n = 34 dogs) and to study the impact of tick protection. the first day of the study, the e. canis pcr were positive in 18 dogs (53%). a. platys was found in one dog and all dogs were negative for babesia spp. after one month of doxycycline treatment, the number of ...201324018180
chemical characterization and acaricide potential of essential oil from aerial parts of tagetes patula l. (asteraceae) against engorged adult females of rhipicephalus sanguineus (latreille, 1806).rhipicephalus sanguineus, commonly known as the brown dog tick, is one of the most widely distributed species of tick. in dogs, it can cause anemia and provide the transmission of pathogenic microorganisms such as babesia canis, ehrlichia canis, hepatozoon canis, anaplasma platys, and mycoplasma haemocanis. to man, it can transmit the intracellular parasites rickettsia rickettsii and rickettsia conorii, the causative agents of the rocky mountain spotted fever in the americas and mediterranean an ...201323508761
tick-borne pathogens and disease in dogs on st. kitts, west indies.between 2009 and 2011, we conducted a case-control study of ticks and tick-associated pathogens affecting dogs on the island of st. kitts, eastern caribbean, including 55 cases of clinically suspected tick-borne disease (tbd) and 110 presumably healthy animals presented for elective surgeries. rhipicephalus sanguineus caused year-round infestations of dogs, and 36% of the dogs in the study were infested at the time of examination. overall, 62% of suspected tbd cases and 24% of presumably healthy ...201323481028
occurrence of ehrlichia canis and anaplasma platys in household dogs from northern parana.canine monocytic ehrlichiosis caused primarily by ehrlichia canis and canine thrombocytic anaplasmosis induced by anaplasma platys are important emerging zoonotic tick-borne diseases of dogs. there is evidence that these pathogens can also affect humans. this study evaluated the presence of e. canis and a. platys in blood samples collected from 256 domiciled dogs in the municipality of jataizinho, located in north region of the state of parana, brazil, by pcr assay. the occurrence of e. canis an ...201323207986
evaluation of a prototype flow cytometry test for serodiagnosis of canine visceral leishmaniasis.diagnosing canine visceral leishmaniasis (cvl) is a critical challenge since conventional immunoserological tests still present some deficiencies. the current study evaluated a prototype flow cytometry serology test, using antigens and fluorescent antibodies that had been stored for 1 year at 4°c, on a broad range of serum samples. noninfected control dogs and leishmania infantum-infected dogs were tested, and the prototype test showed excellent performance in differentiating these groups with h ...201324108778
serosurvey for tick-borne diseases in dogs from the eastern amazon, brazil.canine ehrlichiosis and babesiosis are the most prevalent tick-borne diseases in brazilian dogs. few studies have focused attention in surveying tick-borne diseases in the brazilian amazon region. a total of 129 blood samples were collected from dogs living in the brazilian eastern amazon. seventy-two samples from dogs from rural areas of 19 municipalities and 57 samples from urban stray dogs from santarém municipality were collected. serum samples were submitted to indirect immunofluorescence a ...201323802236
arthropod-borne infections in travelled dogs in europe.pet animal movement is ever increasing within the european union and in that context canine vectorborne infections gained a considerable importance. information on these infections in travelled dogs is nevertheless limited. a first prospective study on vector-borne infections was conducted in 106 dogs travelling from germany to countries in south and south-east europe. the dogs were screened prior to and consecutively up to three times after travel by haematological (giemsa-stained buffy coat sm ...201323477298
identification of babesia species infecting dogs using reverse line blot hybridization for six canine piroplasms, and evaluation of co-infection by other vector-borne pathogens.canine infection by vector-borne hemoparasites is frequent in tropical and sub-tropical areas where exposure to hematophageous ectoparasites is intensive. a reverse line blot (rlb) assay was designed to improve the simultaneous detection of all named canine piroplasm species combined with other vector-borne pathogens of dogs including ehrlichia canis, hepatozoon canis and leishmania infantum common in the mediterranean basin. blood samples of 110 dogs from spain (n=21), portugal (n=14) and israe ...201323017370
mortality in virulent canine babesiosis is associated with a consumptive coagulopathy.the inflammatory response to infection can activate the coagulation system via complex interactions. if uncontrolled, this may lead to a consumptive coagulopathy, a major risk factor for a poor clinical outcome. this prospective observational study was conducted to determine whether consumptive coagulopathy in dogs with babesia rossi infection is related to mortality. seventy-two client-owned dogs diagnosed with canine babesiosis were included. diagnosis was confirmed by polymerase chain reactio ...201323098634
mimicry epitope from ehrlichia canis for interphotoreceptor retinoid-binding protein 201-216 prevents autoimmune uveoretinitis by acting as altered peptide ligand.we report here identification of novel mimicry epitopes for interphotoreceptor retinoid-binding protein (irbp) 201-216, a candidate ocular antigen that causes experimental autoimmune uveoretinitis (eau) in a/j mice. one mimicry epitope from ehrlichia canis (ehc), designated ehc 44-59, induced cross-reactive t cells for irbp 201-216 capable of producing t helper (th)1 and th17 cytokines, but failed to induce eau in a/j mice. in addition, animals first primed with suboptimal doses of irbp 201-216 ...201324029580
are vector-borne pathogen co-infections complicating the clinical presentation in dogs?infection by two or more canine vector-borne disease (cvbd)-causing pathogens is common in subtropical and tropical regions where vectors are plentiful. co-infections may potentiate disease pathogenesis, thereby altering clinical manifestations typically associated with singular infections. these factors complicate diagnosis, treatment and can adversely influence prognosis if the practitioner fails to suspect, document, and treat each concurrent infection. the spectrum of pathogens co-infecting ...201323587324
in vitro culture of a novel genotype of ehrlichia sp. from brazil.ehrlichiae are obligate intracytoplasmic gram-negative, tick-borne bacteria belonging to the anaplasmataceae family. ehrlichioses are considered emerging diseases in both humans and animals. several members of the genus ehrlichia have been isolated and propagated in vitro. this study describes the continuous propagation of a brazilian ehrlichia sp. isolate in ide8 tick cells, canine dh82 cells and bovine aorta cells. initially, the organisms were isolated from the haemolymph of a rhipicephalus ( ...201324589106
inhibitory effect of interferon gamma on frequency of ehrlichia canis-infected cells in vitro.ehrlichia canis is an obligate intracellular bacterium that infects the macrophage-monocyte cells of dogs, causing canine monocytic ehrlichiosis. interferon-γ (ifn-γ), along with other cytokines, mediates the immune response to such intracellular bacterial invasions. to determine the role of ifn-γ in the immunity of dogs to e. canis infection, peripheral blood mononuclear cells (pbmc) and white blood cells (wbc) were collected from e. canis-infected dogs and added to a culture of e. canis in dh8 ...201324148826
ultrastructure of ehrlichia mineirensis, a new member of the ehrlichia genus.recently, we reported the in vitro isolation and the molecular characterization of a new species of ehrlichia (ehrlichia mineirensis) from haemolymph of brazilian rhipicephalus (boophilus) microplus ticks. this organism shows an ortholog of ehrlichia canis major immunogenic protein gp36 with a new structure of tandem repeats. in the present study, we used electron microscopy (high pressure freezing and freeze substitution preparative techniques) to characterize morphologically this new agent gro ...201323998429
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