Publications

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manipulation of neutrophils by porphyromonas gingivalis in the development of periodontitis.the pathogenesis of the chronic periodontal disease is associated with a skewed host inflammatory response to periodontal pathogens, such as porphyromonas gingivalis, that accounts for the majority of periodontal tissue damage. neutrophils are the most abundant leukocytes in periodontal pockets and depending on the stage of the disease, also plentiful pmns are present in the inflamed gingival tissue and the gingival crevice. they are the most efficient phagocytes and eliminate pathogens by a var ...201728589098
differential expression of transcription factors nf-κb and stat3 in periodontal ligament fibroblasts and gingiva of healthy and diseased individuals.pathogens and host mediators can activate transcription factors in periodontal cells to bring about gene level alterations, thereby accentuating the periodontal disease process. nuclear factor-kappa b (nf-κb) and signal transducers and activators of transcription 3 (stat3) are two pivotal transcription factors implicated in chronic inflammatory diseases. but their importance in periodontal pathogenesis has not been investigated in detail. the aim of the present study was to evaluate the expressi ...201728578028
effects of cinnamoyloxy-mammeisin from geopropolis on osteoclast differentiation and porphyromonas gingivalis-induced periodontitis.bone-loss-related diseases such as rheumatoid arthritis, osteomyelitis, osteoporosis, and periodontitis are associated with high rates of morbidity worldwide. these disorders are characterized by an imbalance between the formation and activity of osteoblasts and osteoclasts, leading to bone loss. in this context, we evaluated the effect of cinnamoyloxy-mammeisin (cnm), an anti-inflammatory coumarin found in melipona scutellaris geopropolis, on key targets related to bone remodeling. in the prese ...201728570825
association between anti-porphyromonas gingivalis antibody, anti-citrullinated protein antibodies, and rheumatoid arthritis : a meta-analysis.the aim of this study was to determine the relationship between anti-porphyromonas gingivalis (anti-p. gingivalis) antibody levels and rheumatoid arthritis (ra) and its correlation with anti-citrullinated protein antibodies (acpa).201728555277
plumbagin suppresses chronic periodontitis in rats via down-regulation of tnf-α, il-1β and il-6 expression.chronic periodontitis (cp) is one of the most common oral diseases, which causes alveolar bone absorption and tooth loss in adults. in this study we aimed to investigate the potential of plumbagin (pl), a widely-investigated active compound extracted from the traditional chinese herb plumbago zeylanica l in treating cp. human periodontal ligament stem cells (pdlscs) were used for in vitro studies, whereas an animal model of cp was established in sd rats by ligation+porphyromonas gingivalis (pg) ...201728552911
involvement of cell surface 90 kda heat shock protein (hsp90) in pattern recognition by human monocyte-derived macrophages.heat shock proteins (hsps) are typical intracellular chaperones which also appear on the cell surface and in extracellular milieu. hsp90, which chaperones many proteins involved in signal transduction, is also a regular component of lps-signaling complexes on mϕ. as lps is a prototypical pamp, we speculated that hsp90 is engaged in pattern recognition by professional phagocytes. in this report, we provide the first evidence, to our knowledge, of the geldanamycin (ge)-inhibitable hsp90 on the sur ...201728550115
metformin inhibits p. gingivalis lipopolysaccharide (lps)-influenced inflammatory response in human gingival fibroblasts via regulating activating transcription factor-3 (atf3) expression.chronic periodontitis is one of the most prevalent oral diseases, which is associated with porphyromonas gingivalis (p. gingivalis) lipopolysaccharide (lps) infection, and has profound effects on type 2 diabetes mellitus (dm2). metformin, a well-known antidiabetic agent, has been reported to exert anti-inflammatory effects on various cells. this study aimed to investigate the role of metformin on lps-influenced inflammatory response in human gingival fibroblasts (hgfs).201728548885
dental and periodontal health, and microbiological and salivary conditions in patients with or without diabetes undergoing haemodialysis.the aim of this cross-sectional study was to evaluate the dental and periodontal health, as well as the microbiological and salivary conditions, of patients with and without diabetes mellitus (dm) who are receiving haemodialysis.201728547773
antimicrobial activity of a cationic guanidine compound against two pathogenic oral bacteria.this study evaluated the potential antimicrobial properties of a polyguanidine (catdex) on two oral bacteria. chlorhexidine gluconate 1340 μmol l(-1) (chx 0.12%) was used as control. streptococcus mutans (s. mutans) and porphyromonas gingivalis (p. gingivalis) were grown in bhi media. bacterial sensitivity and antimicrobial activity were determined by the minimum inhibitory concentration (mic) and kirby-bauer methods. to study side effects, that is, toxicity, dental pulp stem cells (dpscs) were ...201728546811
phenotype and function of myeloid-derived suppressor cells induced by porphyromonas gingivalis infection.porphyromonas gingivalis, a major etiologic agent of periodontitis, has been reported to induce the expansion of myeloid-derived suppressor cells (mdsc); however, little is known regarding the subpopulations of mdsc expanded by p. gingivalis infection. flow cytometry was used to evaluate bone marrow and spleen cells from mice infected with p. gingivalis and controls for surface expression of cd11b, ly6g, and ly6c. to characterize the phenotype of mdsc subpopulations induced by infection, cells w ...201728533469
impact of docosahexaenoic acid therapy on subgingival plaque microbiota.oral docosahexaenoic acid (dha) + aspirin therapy has been shown to reduce periodontal probing depth (pd) and local inflammatory mediators in gingival crevicular fluid (gcf) among patients with untreated chronic periodontal disease. whether dha + aspirin therapy influences specific bacterial burden in this setting is unknown. thus, the aim of this study is to evaluate the impact of dha with low-dose aspirin therapy on periodontal bacterial profile in patients with periodontitis.201728517974
one-step hydrothermal synthesis of photoluminescent carbon nanodots with selective antibacterial activity against porphyromonas gingivalis.carbon dots (cds) with high quantum yield (qy), low toxicity and good biocompatibility are the major quests of biomedical research. in this paper, we develop a green, simple hydrothermal approach to synthesize water-soluble, nontoxic, highly photoluminescent carbon nanodots (cnds) from metronidazole, which possess selective antibacterial activity against obligate anaerobes for the first time. metronidazole was used as a sole precursor to prepare cnds at 250 °c (cnds-250) for 8 h. after the cnds- ...201728513713
conditioned medium from relapsing-remitting multiple sclerosis patients reduces the expression and release of inflammatory cytokines induced by lps-gingivalis in thp-1 and mo3.13 cell lines.the present research was aimed at evaluating the effect of the conditioned medium (cm) from human periodontal ligament stem cells (hpdlscs) obtained from healthy donors (hpdlscs-cm) and from relapsing-remitting multiple sclerosis patients (rr-ms-cm) on inflammatory response induced by porphyromonas gingivalis lipopolysaccharide (lps-g) in a monocytoid human cell line (thp-1) and human oligodendrocyte cell line (mo3.13). human periodontal ligament biopsies were carried out from control donor pati ...201728511117
the role of proteinase-activated receptors 1 and 2 in the regulation of periodontal tissue metabolism and disease.proteinase-activated receptors 1 (par1) and 2 (par2) are the most highly expressed members of the par family in the periodontium. these receptors regulate periodontal inflammatory and repair processes through their activation by endogenous and bacterial enzymes. par1 is expressed by the periodontal cells such as human gingival fibroblasts, gingival epithelial cells, periodontal ligament cells, osteoblasts, and monocytic cells and can be activated by thrombin, matrix metalloproteinase 1 (mmp-1), ...201728503577
the aggravation of arthritis by periodontitis is dependent of il-17 receptor a activation.to evaluate whether porphyromonas gingivalis-induced periodontitis aggravates the antigen-induced arthritis (aia) model, and whether this effect is dependent on the th17/il-17 signalling pathway.201728498497
inactive gingipains from p. gingivalis selectively skews t cells toward a th17 phenotype in an il-6 dependent manner.gingipain cysteine proteases are considered key virulence factors of porphyromonas gingivalis. they significantly influence antibacterial and homeostatic functions of macrophages, neutrophils, the complement system, and cytokine networks. recent data indicate the role of p. gingivalis in t cell differentiation; however, the involvement of gingipains in this process remains elusive. therefore, the aim of this study was to investigate the contribution of danger signals triggered by the gingipains ...201728497028
pkc, erk/p38 map kinases and nf-κb targeted signalling play a role in the expression and release of il-1β and cxcl8 in porphyromonas gingivalis-infected thp1 cells.porphyromonas gingivalis is a keystone pathogen in periodontitis and is gaining importance in cardiovascular pathogenesis. protease-activated receptors (pars), toll-like receptors (tlrs) and nucleotide-binding oligomerization domain (nod) on monocytes recognize the structural components on p. gingivalis, inducing inflammatory intermediates. here, we elucidate the modulation of pars, tlrs, nods, and the role of mapk and nf-κb in il-1β and cxcl8 release. thp1 cells were stimulated with p. gingival ...201728493507
theoretical insights into the protonation states of active site cysteine and citrullination mechanism of porphyromonas gingivalis peptidylarginine deiminase.porphyromonas gingivalis peptidylarginine deiminase (ppad) catalyzes the citrullination of peptidylarginine, which plays a critical role in the rheumatoid arthritis (ra) and gene regulation. for a better understanding of citrullination mechanism of ppad, it is required to establish the protonation states of active site cysteine, which is still a controversial issue for the members of guanidino-group-modifying enzyme superfamily. in this work, we first explored the transformation between the two ...201728486790
using tn-seq to identify pigmentation-related genes of porphyromonas gingivalis: characterization of the role of a putative glycosyltransferase.cellular pigmentation is an important virulence factor of the oral pathogen porphyromonas gingivalis pigmentation has been associated with many bacterial functions, including but not limited to colonization, maintaining a local anaerobic environment by binding oxygen molecules, and defense against reactive oxygen species (ros) produced by immune cells. pigmentation-associated loci identified to date have involved lipopolysaccharide, fimbriae, and heme acquisition and processing. we utilized a tr ...201728484050
[study on the role of nalp3 inflammasome in porphyromonas gingivalis lipopolysaccharide induced raw264.7].objective: to illuminate the effect of nalp3 inflammasome on regulating the expression of cytokines of macrophages in periodontitis. methods: raw264.7 cells were cultured and divided into three groups. the first group stayed normal as control, the second group was stimulated by 1 mg/l porphyromonas gingivalis (pg) lipopolysaccharide (lps), the third group was pretreated with ac-yvad-cmk (caspase-1 inhibitor) before stimulated with 1 mg/l pg lps. raw264.7 cells pretreated with various concentrati ...201728482444
enhanced bioactivity and bacteriostasis of surface fluorinated polyetheretherketone.although polyetheretherketone (peek) has been considered as a potential orthopedic and dental application material due to its similar elastic modulus as bones, inferior osseointegration and bacteriostasis of peek hampers its clinical application. in this work, fluorinated peek was constructed via plasma immersion ion implantation (piii) followed by hydrofluoric acid treatment to ameliorate the osseointegration and antibacterial properties of peek. the surface microstructure, composition, and hyd ...201728474880
new approaches to combat porphyromonas gingivalis biofilms.in nature, bacteria predominantly reside in structured, surface-attached communities embedded in a self-produced, extracellular matrix. these so-called biofilms play an important role in the development and pathogenesis of many infections, as they are difficult to eradicate due to their resistance to antimicrobials and host defense mechanisms. this review focusses on the biofilm-forming periodontal bacterium porphyromonas gingivalis. current knowledge on the virulence mechanisms underlying p. gi ...201728473880
camelid nanobodies used as crystallization chaperones for different constructs of porm, a component of the type ix secretion system from porphyromonas gingivalis.porm is a membrane protein that is involved in the assembly of the type ix secretion system (t9ss) in porphyromonas gingivalis, a major bacterial pathogen that is responsible for periodontal disease in humans. in the context of structural studies of porm to better understand t9ss assembly, four camelid nanobodies were selected, produced and purified, and their specific interaction with the n-terminal or c-terminal part of the periplasmic domain of porm was investigated. diffracting crystals were ...201728471361
identification of streptococcus cristatus peptides that repress expression of virulence genes in porphyromonas gingivalis.dental plaque is a complex multispecies biofilm, and is a direct precursor of periodontal disease. the virulence of periodontal pathogens, such as porphyromonas gingivalis, is expressed in the context of this polymicrobial community. previously, we reported an antagonistic relationship between streptococcus cristatus and p. gingivalis, and identified arginine deiminase (arca) of s. cristatus as the signaling molecule to which p. gingivalis responds by repressing the expression and production of ...201728469253
chair-side detection of prevotella intermedia in mature dental plaque by its fluorescence.prevotella intermedia/nigrescens is one of the well-known pathogens causing periodontal diseases, and the red florescence excited by the visible blue light caused by the protoporphyrin ix in the bacterial cells could be useful for the chair-side detection. the aim of this study was to evaluated levels of periodontal pathogen, especially p. intermedia in clinical samples of red fluorescent dental plaque.201728457849
can better management of periodontal disease delay the onset and progression of alzheimer's disease?a risk factor relationship exists between periodontal disease and alzheimer's disease (ad) via tooth loss, and improved memory following dental intervention. this links the microbial contribution from indigenous oral periodontal pathogens to the manifestation of chronic conditions, such as ad. here, we use porphyromonas gingivalis infection to illustrate its effect on mental health. p. gingivalis infection, in its primary sub-gingival niche, can cause polymicrobial synergy and dysbiosis. dysbios ...201728453484
oral infection with porphyromonas gingivalis induces peri-implantitis in a murine model: evaluation of bone loss and the local inflammatory response.peri-implantitis is a major health concern, with unclear pathogenesis, and with no accessible animal models. our aim was to establish a mouse model for peri-implantitis and to investigate mediators of inflammation.201728453225
distinct roles for dietary lipids and porphyromonas gingivalis infection on atherosclerosis progression and the gut microbiota.mounting evidence in humans supports an etiological role for the microbiota in inflammatory atherosclerosis. atherosclerosis is a progressive disease characterized by accumulation of inflammatory cells and lipids in vascular tissue. while retention of lipoprotein into the sub-endothelial vascular layer is believed to be the initiating stimulus leading to the development of atherosclerosis, activation of multiple pathways related to vascular inflammation and endothelial dysfunction sustain the pr ...201728442421
periodontal-derived mesenchymal cell sheets promote periodontal regeneration in inflammatory microenvironment.in this study, we investigated the periodontal regenerative potential of dental follicle cell (dfc) sheets and periodontal ligament cell (pdlc) sheets in the simulating inflammatory microenvironment of periodontitis, to confirm their regenerative potential for clinical application and explain the possible mechanism. the biological characteristics of dfc sheets and pdlc sheets were explored in lipopolysaccharide from porphyromonas gingivalis (p. gingivalis lps)-induced inflammation microenvironme ...201728437177
inhibitory activities of selected sudanese medicinal plants on porphyromonas gingivalis and matrix metalloproteinase-9 and isolation of bioactive compounds from combretum hartmannianum (schweinf) bark.periodontal diseases are one of the major health problems and among the most important preventable global infectious diseases. porphyromonas gingivalis is an anaerobic gram-negative bacterium which has been strongly implicated in the etiology of periodontitis. additionally, matrix metalloproteinases-9 (mmp-9) is an important factor contributing to periodontal tissue destruction by a variety of mechanisms. the purpose of this study was to evaluate the selected sudanese medicinal plants against p. ...201728427461
detection of th17/treg cells and related factors in gingival tissues and peripheral blood of rats with experimental periodontitis.this study aimed to investigate the role and the possible mechanisms involved in the immunoregulation of experimental periodontitis by th17/treg.201728392902
subchronic infection of porphyromonas gingivalis and tannerella forsythia stimulates an immune response but not arthritis in experimental murine model.studies have proposed that porphyromonas gingivalis (pg) and tannerella forsythia (tf) promote a nonspecific inflammatory response that could produce systemic disease. oral inoculation of pg and tf on the immune and arthritis response was evaluated in balb/c mice divided into four groups: (1) sham; (2) food contaminated with pg/tf; (3) complete freund's adjuvant (cfa) + pg/tf; and (4) cfa alone. cfa was administered subcutaneously on days 1 and 14. the arthritis response was monitored for 21 day ...201728676826
black-pigmented anaerobic bacteria associated with ovine periodontitis.periodontitis is a polymicrobial infectious disease that causes occlusion change, tooth loss, difficulty in rumination, and premature culling of animals. this study aimed to detect species of the genera porphyromonas and prevotella present in the periodontal pocket of sheep with lesions deeper than 5mm (n=14) and in the gingival sulcus of animals considered periodontally healthy (n=20). the presence of microorganisms was evaluated by polymerase chain reaction (pcr) using specific primers for por ...201728619155
disulfide bonds: a key modification in bacterial extracytoplasmic proteins.disulfide bonds are a common posttranslational modification that contributes to the folding and stability of extracytoplasmic proteins. almost all organisms, from eukaryotes to prokaryotes, have evolved enzymes to make and break these bonds. accurate and efficient disulfide bond formation can be vital for protein function; therefore, the enzymes that catalyze disulfide bond formation are involved in multiple biological processes. recent advances clearly show that oral bacteria also have the abil ...201728797211
risk of superinfection in peri-implantitis after systemic broad spectrum antibiotics.peri-implant disease has developed over the last few years as a complication that is often difficult to resolve. the disease process is mainly attributed to bacterial infection. proposed combined therapies use broad-spectrum antibiotics to halt its progression. a major associated risk is the undetected development of superinfections that are difficult to eradicate. a group of healthy individuals with advanced peri-implantitis (pi) were referred for evaluation due to severe, rapidly progressive b ...201728854287
quantification by qpcr of pathobionts in chronic periodontitis: development of predictive models of disease severity at site-specific level.currently, there is little evidence available on the development of predictive models for the diagnosis or prognosis of chronic periodontitis based on the qpcr quantification of subgingival pathobionts. our objectives were to: (1) analyze and internally validate pathobiont-based models that could be used to distinguish different periodontal conditions at site-specific level within the same patient with chronic periodontitis; (2) develop nomograms derived from predictive models. subgingival plaqu ...201728848499
microbiological response to periodontal therapy and multivariable prediction of clinical outcome.this study assessed the microbiological effects of a 2-phase antimicrobial periodontal therapy and tested microbiological, clinical, and biological markers as prognostic indicators for clinical success.201728844191
relatively low invasive capacity of porphyromonas gingivalis strains into human gingival fibroblasts in vitro.bacterial invasion into host cells is a common strategy to escape the host immune system. gingival fibroblasts (gfs) are the most predominant non-phagocytic cell type in gingival connective tissue. therefore, invasion into gfs was thought to be the first strategy for the survival of porphyromonas gingivalis. the present study compared the invasive ability of p. gingivalis into gfs with those of other red-complex and relatively less pathogenic bacterial strains, especially fusobacterium nucleatum ...201728841474
the oral microbiome and adverse pregnancy outcomes.significant evidence supports an association between periodontal pathogenic bacteria and preterm birth and preeclampsia. the virulence properties assigned to specific oral pathogenic bacteria, for example, fusobacterium nucleatum, porphyromonas gingivalis, filifactor alocis, campylobacter rectus, and others, render them as potential collaborators in adverse outcomes of pregnancy. several pathways have been suggested for this association: 1) hematogenous spread (bacteremia) of periodontal pathoge ...201728848365
periodontal microbioma and rheumatoid arthritis: the role of porhyromonas gingivalis.rheumatoid arthritis is a disease, which can be described as an autoimmune response after molecular mimicry caused by infective agents. the current study aims at evaluating the correlation between rhematoid arthritis (ra) and periodontal disease (pd), with special attention to the microbioma detected in the gums. thirty-four patients with rd were recruited into the current study. among rheumatic parameters, rheumatoid factor (rf), anti-citrullinated protein antibody (ccp), hla-bdr1 and das28 wer ...201728691459
hospital indicators. 20164820306
technical aspects of dr. kryter's paper "impairment to hearing from exposure to noise" with respect to the niosh statistics. 20164712552
the effects of diethylstilboestrol on obesity in mice. 20164760785
the traditional shona's attitude to medicine. 20165080379
[incision for reduction of tension in burned hands]. 20165010206
characterization of the factor deficient in the hunter syndrome by polyacrylamide gel electrophoresis. 20164987382
pyrophosphate phosphohydrolase in c3hba mammary tumors of mice. 20164956748
physicians and medical electronic equipment: a critical review of our modus operandi. 20164812377
[traumatic lesions of the aorta and the large basal vessels]. 20164925815
rna synthesis during mammalian myogenesis in culture. 20165066247
oliguric acute renal failure in malignant hypertension. 20165058504
the ionic requirements for the initiation of action potentials in insect muscle fibers.electrical properties of locust leg muscle fibers were studied by means of intracellular electrodes. in most fibers, a depolarizing current pulse initiated a local response. a delayed decrease in membrane resistance appeared with more than about 10 mv depolarization. in some fibers a regenerative response also was found. membrane constants were measured, applying the short cable model. the value of the space constant lambda was 1.6 mm and the calculated value of r(m) was about 1750 ohm cm(2). ac ...20165058471
population screening for coronary risk factors. 20165075313
[stereochemistry of ephedrine-like compounds. absolute configuration of the four isomeric cis- and trans-6-amino-6,7,8,9-tetrahydro-5h-benzocycloheptenols-(5)]. 20165053529
clinical application of anal sphincter electromyography. 20165047524
correlation of endometrial biopsy and plasma progesterone in infertile women. 20165039235
[evaluation of the toxicity of new n-chloroamides of aromatic sulfonic acids. effect of selected compounds on internal organs in white mice]. 20165034476
applied oxygen therapy. 20165027532
cholesterol embolization and spinal infarction following aortic catheterization. 20165025015
chronic synovitis as a manifestation of calcium crystal deposition disease. 20165100635
demand for hospital confinement in birmingham. 20165037517
managing delayed puberty. 20165025219
the public health nurse and the long-term neurologic patient. 20164976368
structure and mode of action of glycoproteins from an antarctic fish. 20165031164
a cesarean section on a gelada baboon. 20164967714
leukemic reticuloendotheliosis. 20164943601
[20th anniversary of professor slobodan djordjević as editor of the serbian archives for general medicine]. 20164928203
historical review of the z- and w-plasty. revisions of linear scars. 20164935913
septal dermoplasty--its several uses. 20164919060
the management of obesity: some recent concepts. 20164897933
clinical and laboratory study of a new diuretic agent ("vectren") in pregnancy: a comparison with a diuretic agent in current use ("enduron"). 20164889612
letter: animal experiments. 20164832241
fresh-water near-drowning: radiological aspects. 20164832512
intestinal crypt survival and total and per crypt levels of proliferative cellularity following irradiation: single x-ray exposures. 20165087758
[pulmonary resection in tuberculosis. bronchial healing: a comparative study]. 20164906878
letter: isoniazid. 20164843589
the isolation and identification of the ergoline alkaloids from ipomoea muelleri. 20164818014
lymphocyte toxicity test in clinical melanoma. 20164804311
simple and specific assay of penicillins by ir spectrophotometry in deuterium oxide and dimethyl sulfoxide solutions. 20164813242
on the development of the girl's wish for a child. 20164807508
origin of margarine. 20164929301
president's message. 20165062586
the spread of comprehensive care through under-fives' clinics. 20164784052
selective sacral nerve blocks in the management of "uninhibited neurogenic bladder". 20164836921
letter: the solubility and nucleation of cholesterol in bile. 20164810921
[clinical and functional study of aerosol dexamethasone isonicotinate in chronic bronchial asthma (author's transl)]. 20164775917
[intestinal dystopia]. 20164785262
clinical studies on radiation inquires resulting from accidental exposure to an iridium-192 radiographic source. 20164766246
proceedings: detection and management of "early breast cancer". 20164764907
the medical social worker in the coronary care unit. 20164776208
pancoast tumour. report of a case. 20164773545
patient exposures in diagnostic radiology: protection problems of current concern. a statement issued by the international commission on radiological protection (icrp). july 1973. 20164771834
smallpox in the newborn. a case report. 20164746253
the effects of low doses of depot estradiol and testosterone in teenagers with ovarian failure and turner's syndrome. 20164742538
experimental investigation of lsd as a psychotherapeutic adjunct. 20164879643
the effect of continuous epidural blockade on plasma 11-hydroxycorticosteroid concentrations in labour. 20164752989
concurrent schizophrenia-like psychosis in monozygous twins suffering from cns disorder. 20164716065
transplant problems. 20164739346
[the porphyric liver. correlations among the histopathology, clinical manifestations and the laboratory. personal experience]. 20164805248
chaotic atrial rhythm. 20165033407
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