Publications

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draft genome sequence of non-shiga toxin-producing escherichia coli o157 nccp15738.the non-shiga toxin-producing escherichia coli (non-stec) o157 is a pathogenic strain that cause diarrhea but does not cause hemolytic-uremic syndrome, or hemorrhagic colitis. here, we present the 5-mb draft genome sequence of non-stec o157 nccp15738, which was isolated from the feces of a korean patient with diarrhea, and describe its features and the structural basis for its genome evolution.201627096008
evaluation of pcr based assays for the improvement of proportion estimation of bacterial and viral pathogens in diarrheal surveillance.diarrhea can be caused by a variety of bacterial, viral and parasitic organisms. laboratory diagnosis is essential in the pathogen-specific burden assessment. in the pathogen spectrum monitoring in the diarrheal surveillance, culture methods are commonly used for the bacterial pathogens' detection whereas nucleic acid based amplification, the non-cultural methods are used for the viral pathogens. different methodology may cause the inaccurate pathogen spectrum for the bacterial pathogens because ...201627065958
microbiological quality assessment by pcr and its antibiotic susceptibility in mangrove crabs (ucides cordatus) from guanabara bay, rio de janeiro, brazil.the bacteriological quality of crabs from three different mangroves (itaóca, suruí, and piedade) from rio de janeiro state, brazil, was investigated using conventional and molecular methods. the results revealed high counts for total coliforms in meat and hepatopancreas samples. pcr analyses identified 25 escherichia coli colonies in the itaóca, piedade, and suruí samples, detecting 13 enterotoxigenic colonies and 9 enteroaggregative colonies. respectively, 12, 11, and 21 vibrio parahaemolyticus ...201627065187
the first description of complete invertebrate arginine metabolism pathways implies dose-dependent pathogen regulation in apostichopus japonicus.in this study, three typical members representative of different arginine metabolic pathways were firstly identified from apostichopus japonicus, including nitric oxide synthase (nos), arginase, and agmatinase. spatial expression analysis revealed that the ajnos transcript presented negative expression patterns relative to those of ajarginase or ajagmatinase in most detected tissues. furthermore, vibrio splendidus-challenged coelomocytes and intestine, and lps-exposed primary coelomocytes could ...201627032691
molecular and immune response characterizations of il-6 in large yellow croaker (larimichthys crocea).interleukin-6 (il-6) is a multifunctional inflammatory cytokine which exists in multiple tissues and cell lines. in the present study, the full-length cdna and the genomic sequence of il-6 (lcil-6) were cloned from large yellow croaker, larimichthys crocea. the full-length cdna of lcil-6 was 1066 base pairs (bp), containing an open reading frame (orf) of 678 bp encoding for 225 amino acids, a 5' untranslated region (utr) of 71 bp and a 3' utr of 317 bp. the predicted lcil-6 protein included a 24 ...201626868214
functions of vpa1418 and vpa0305 catalase genes in growth of vibrio parahaemolyticus under oxidative stress.the marine foodborne enteropathogen vibrio parahaemolyticus has four putative catalase genes. the functions of two kate-homologous genes, kate1 (vpa1418) and kate2 (vpa0305), in the growth of this bacterium were examined using gene deletion mutants with or without complementary genes. the growth of the mutant strains in static or shaken cultures in a rich medium at 37°c or at low temperatures (12 and 4°c), with or without competition from escherichia coli, did not differ from that of the parent ...201626746716
structural characterization and biomedical properties of sulfated polysaccharide from the gladius of sepioteuthis lessoniana (lesson, 1831).sulfated polysaccharide was extracted from the internal shell (gladius) of sepioteuthis lessoniana. the sulfated polysaccharide contained 61.3% of carbohydrate, 0.8% of protein, 28.2% of ash and 1.33% of moisture respectively. the elemental composition was analyzed using chns/o analyzer. the molecular weight of sulfated polysaccharide determined through page was found to be as 66 kda. monosaccharides analysis revealed that sulfated polysaccharide was composed of rhamnose, galactose, xylose and g ...201626724686
green synthesis of silver nanoparticles by bacillus methylotrophicus, and their antimicrobial activity.the synthesis of silver nanoparticles (agnps) by microorganisms is an area attracting growing interest in nanobiotechnology, due to the applications of these nanoparticles in various products including cosmetics and biosensors, and in the biomedical, clinical, and bioimaging fields as well. various microorganisms have been found to be able to synthesize agnps when silver salts are supplied in the reaction system. the main objectives of this study were to evaluate the efficiency of synthesis of a ...201625749281
case-control study of diarrheal disease etiology in individuals over 5 years in southwest china.acute diarrhea is one of the major public health problems worldwide. most of studies on acute diarrhea have been made on infants aged below 5 years and few efforts have been made to identify the etiological agents of acute diarrhea in people over five, especially in china.201627891182
impact of co-infections with enteric pathogens on children suffering from acute diarrhea in southwest china.acute diarrhea is a global health problem, resulting in high morbidity and mortality in children. it has been suggested that enteric pathogen co-infections play an important role in gastroenteritis, but most research efforts have only focused on a small range of species belonging to a few pathogen groups. this study aimed to assess the impact of co-infections with a broad range of enteric pathogens on children aged below five years who suffer from acute diarrhea in southwest china.201627349521
an improved multiplex real-time sybr green pcr assay for analysis of 24 target genes from 16 bacterial species in fecal dna samples from patients with foodborne illnesses.here, we developed a new version of our original screening system (rapid foodborne bacterial screening 24; rfbs24), which can simultaneously detect 24 genes of foodborne pathogens in fecal dna samples. this new version (rfbs24 ver. 5) detected all known stx2 subtypes, enterotoxigenic escherichia coli (sth genotype), and vibrio parahaemolyticus (trh2), which were not detected by the original rfbs24 assay. the detection limits of rfbs24 ver. 5 were approximately 5.6 × 10(-2)-5.6 × 10(-5) (ng dna)/ ...201626166499
a specific antimicrobial protein cap-1 from pseudomonas sp. isolated from the jellyfish cyanea capillata.a bacterium strain, designated as cmf-2, was isolated from the jellyfish cyanea capillata and its culture supernatant exhibited a significant antimicrobial activity. the strain cmf-2 was identified as pseudomonas sp. based on the morphological, biochemical and physiological characteristics as well as 16s rrna sequence analysis. in this study, an antimicrobial protein, named as cap-1, was isolated from the culture of cmf-2 through ammonium sulfate precipitation and gel filtration chromatography. ...201626529191
recombinant expression of a modified shrimp anti-lipopolysaccharide factor gene in pichia pastoris gs115 and its characteristic analysis.anti-lipopolysaccharide factors (alfs) with a lps-binding domain (lbd) are considered to have broad spectrum antimicrobial activities and certain antiviral properties in crustaceans. fcalf2 was one isoform of alfs isolated from the chinese shrimp fenneropenaeus chinensis. our previous study showed that a modified lbd domain (named lbdv) of fcalf2 exhibited a highly enhanced antimicrobial activity. in the present study, a modified fcalf2 gene (mfcalf2), in which the lbd was substituted by lbdv, w ...201627517939
gfp tagged vibrio parahaemolyticus dahv2 infection and the protective effects of the probiotic bacillus licheniformis dahb1 on the growth, immune and antioxidant responses in pangasius hypophthalmus.in this study, the pathogenicity of gfp tagged vibrio parahaemolyticus dahv2 and the protective effect of the probiotic strain, bacillus licheniformis dahb1 was studied on the asian catfish, pangasius hypophthalmus. the experiment was carried out for 24 days with three groups and one group served as the control (without treatment). in the first group, p. hypophthalmus was orally infected with 1 ml of gfp tagged v. parahaemolyticus dahv2 at two different doses (10(5) and 10(7) cfu ml(-1)). in the ...201626952171
biosynthesis of silver nanoparticles using a probiotic bacillus licheniformis dahb1 and their antibiofilm activity and toxicity effects in ceriodaphnia cornuta.in the present study, we synthesized and characterized a probiotic bacillus licheniformis cell free extract (blcfe) coated silver nanoparticles (blcfe-agnps). these blcfe-agnps were characterized by uv-visible spectrophotometer, xrd, edx, ftir, tem and afm. a strong surface plasmon resonance centered at 422 nm in uv-visible spectrum indicates the formation of agnps. the xrd spectrum of silver nanoparticles exhibited 2θ values corresponding to the silver nanocrystal. tem and afm showed the agnps ...201626802520
biogenic silver and gold nanoparticles synthesized using red ginseng root extract, and their applications.in the present study, we report a green methodology for the synthesis of silver and gold nanoparticles, using the root extract of the herbal medicinal plant korean red ginseng. the silver and gold nanoparticles were synthesized within 1 h and 10 min respectively. the nanoparticles generated were not aggregated, and remained stable for a long time, which suggests the nature of nanoparticles. the phytochemicals and ginsenosides present in the root extract assist in reducing and stabilizing the syn ...201625706249
the development of a green approach for the biosynthesis of silver and gold nanoparticles by using panax ginseng root extract, and their biological applications.the biosynthesis of nanoparticles has received attention because of the development of economic and environmentally friendly technology for the synthesis of nanoparticles. the study develops a convenient method for the green synthesis of silver and gold nanoparticles by utilizing fresh root extract of the four-year old panax ginseng plant, and evaluated the antimicrobial applications of silver nanoparticles against pathogenic microorganisms. p. ginseng is a well-known herbal medicinal plant, and ...201625771716
a preliminary study on the antibacterial mechanism of tegillarca granosa hemoglobin by derived peptides and peroxidase activity.the blood clam, tegillarca granosa, is one of the few bivalve molluscs containing hemoglobin (hb). in the present study, we purified two types of t. granosa hemoglobin, tg-hbi and tg-hbii, using size exclusion chromatography and measured their antibacterial and peroxidase activities. we also tested antibacterial activities of peptides prepared by trypsin digestion of purified tg-hb and reversed-phase high-performance liquid chromatography purification. purified tg-hbi and tg-hbii showed antibact ...201626876330
a rapid and simple real-time pcr assay for detecting foodborne pathogenic bacteria in human feces.a rapid, simple method for detecting foodborne pathogenic bacteria in human feces is greatly needed. here, we examined the efficacy of a method that employs a combination of a commercial pcr master mix, which is insensitive to pcr inhibitors, and a dna extraction method which used sodium dodecyl benzene sulfonate (sdbs), and tween 20 to counteract the inhibitory effects of sdbs on the pcr assay. this method could detect the target genes (stx1 and stx2 of enterohemorrhagic escherichia coli, inva ...201626902211
green synthesis of multifunctional silver and gold nanoparticles from the oriental herbal adaptogen: siberian ginseng.pharmacologically active stem of the oriental herbal adaptogen, siberian ginseng, was employed for the ecofriendly synthesis of siberian ginseng silver nanoparticles (sg-agnps) and siberian ginseng gold nanoparticles (sg-aunps). first, for metabolic characterization of the sample, liquid chromatography-tandem mass spectrometry analysis (indicated the presence of eleutherosides a and e), total phenol content, and total reducing sugar were analyzed. second, the water extract of the sample mediated ...201627468232
extracellular synthesis of silver and gold nanoparticles by sporosarcina koreensis dc4 and their biological applications.the present study highlights the microbial synthesis of silver and gold nanoparticles by sporosarcina koreensis dc4 strain, in an efficient way. the synthesized nanoparticles were characterized by ultraviolet-visible spectrophotometry, which displayed maximum absorbance at 424nm and 531nm for silver and gold nanoparticles, respectively. the spherical shape of nanoparticles was characterized by field emission transmission electron microscopy. the energy dispersive x-ray spectroscopy and elemental ...201626992796
a strategic approach for rapid synthesis of gold and silver nanoparticles by panax ginseng leaves.the study highlights the synthesis of gold nanoparticles and silver nanoparticles by fresh leaves of panax ginseng, an herbal medicinal plant. the reduction of auric chloride and silver nitrate led to the formation of gold and silver nanoparticles within 3 and 45 min, at 80°c, respectively. the developed methodology was rapid, facile, ecofriendly and the utmost significant is quite economical, which did not require subsequent processing for reduction or stabilization of nanoparticles. the nanopa ...201626698271
characterization and antimicrobial application of biosynthesized gold and silver nanoparticles by using microbacterium resistens.various microorganisms were found to be cable of synthesizing gold and silver nanoparticles when gold and silver salts were supplied in the reaction system. the main objective of this study was to evaluate the extracellular synthesis of gold and silver nanoparticles by the type strain microbacterium resistens(t) [kacc14505]. the biosynthesized gold and silver nanoparticles were characterized by ultraviolet-visible spectroscopy (uv-vis), field emission transmission electron micrograph (fe-tem), e ...201626597594
weissella oryzae dc6-facilitated green synthesis of silver nanoparticles and their antimicrobial potential.nanoparticles and nanomaterials are at the prominent edge of the rapidly developing field of nanotechnology. recently, nanoparticle synthesis using biological resources has been found to be a new area with considerable prospects for development. biological systems are the masters of ambient condition chemistry and are able to synthesize nanoparticles by utilizing metal salts. in the perspective of the current initiative to develop green technologies for the synthesis of nanoparticles, microorgan ...201626212222
[monitoring and research on pathogen spectrum in patients with acute diarrhea from sentinel hospital of zhejiang province during 2009 to 2014].objective: to explore pathogen spectrum constitution of acute diarrhea in outpatient and emergency of zhejiang province, and provide basis for treatment, prevention and control of the disease. methods: during january 2009 to december 2014, we selected seven sentinel hospitals in different regions of zhejiang, monitored and researched on pathogen spectrum in patients with acute diarrhea from outpatient and emergency. we recorded patients' personal basic information, the main symptoms and signs, a ...201628057113
two endoplasmic reticulum proteins (calnexin and calreticulin) are involved in innate immunity in chinese mitten crab (eriocheir sinensis).calnexin (cnx) and calreticulin (crt), which are important chaperones in the endoplasmic reticulum (er), participate in the folding and quality control of client proteins. cnx and crt identified from chinese mitten crab (eriocheir sinensis) are designated as escnx and escrt, respectively. escnx and escrt are expressed in the hemocyte, hepatopancrea, gill, and intestine at the mrna and protein level. immunofluorescence analysis indicated that escnx and escrt are located in the er. moreover, the m ...201627279413
antimicrobial activities of secondary metabolites and phylogenetic study of sponge endosymbiotic bacteria, bacillus sp. at agatti island, lakshadweep archipelago.twenty-one species of sponges were recorded under the class of demospongiae and calcareous sponges of which 19 species were new to agatti reef. a total of 113 sponge endosymbiotic bacterial strains were isolated from twenty-one species of sponges and screened for antimicrobial activity. five bacterial strains of sponge endosymbiotic bacteria (seb) namely seb32, seb33, seb36, seb43 and seb51 showed antimicrobial activity against virulent marine fish pathogens such as vibrio alginolyticus, vibrio ...201628352539
establishment and characterization of a skin epidermal cell line from mud loach, misgurnus anguillicaudatus, (mase) and its interaction with three bacterial pathogens.a continuous skin epidermal cell line from mud loach (misgurnus anguillicaudatus) (mase cell line) was established with its application in bacteria infection demonstrated in this study. primary mase cell culture was initiated at 26 °c in dulbecco's modified eagle medium/f12 medium (1:1; ph7.2) supplemented with 20% fetal bovine serum (fbs). the primary mase cells in spindle morphology proliferated into a confluent monolayer within 2 weeks, and were continuously subcultured even in 10% fbs- dmem/ ...201627288257
molecular characteristics of lysozyme g in euryglossa orientalis; cdna cloning, phylogenic analysis, physicochemical properties and tissue gene expression.lysozymes are the key molecules of innate immune system against bacterial infections. in the present study, we identified the molecular characteristics, physicochemical properties, antibacterial activity, evolutionary relationship and tissue expression pattern of g-type lysozyme in euryglossa orientalis (euor lysg). the full-length euor lysg cdna is composed of 588 nucleotides and an open reading frame encoding a protein with 195 amino acids with more than 65 % identity to g-type lysozyme of sol ...201627393386
effect of acidic electrolyzed water-induced bacterial inhibition and injury in live clam (venerupis philippinarum) and mussel (mytilus edulis).the effect of acidic electrolyzed water (aew) on inactivating escherichia coli o104:h4, listeria monocytogenes, aeromonas hydrophila, vibrio parahaemolyticus and campylobacter jejuni in laboratory contaminated live clam (venerupis philippinarum) and mussel (mytilus edulis) was investigated. the initial levels of bacterial contamination were: in clam 4.9 to 5.7log10cfu/g, and in mussel 5.1 to 5.5log10cfu/g. two types of aew were used for treatment time intervals of 1 and 2h: strong (saew) with an ...201627208583
antibacterial activity of litsea cubeba (lauraceae, may chang) and its effects on the biological response of common carp cyprinus carpio challenged with aeromonas hydrophila.the aims of this study were to characterize the antibacterial activity and the chemotype of litsea cubeba leaf essential oil (eo) harvested in north vietnam and to investigate the biological effects induced by the leaf powder on growth, nonspecific immunity and survival of common carp (cyprinus carpio) challenged with aeromonas hydrophila.201627124660
identification of polyvalent protective immunogens from outer membrane proteins in vibrio parahaemolyticus to protect fish against bacterial infection.vaccination is one of the most effective and economic way to prevent infectious diseases in aquaculture. the development of effective vaccines, however, is still limited, especially for polyvalent vaccines, which are against multiple species. with this regard, identification of polyvalent protective immunogens, serving as polyvalent vaccines, became a key step in vaccine development. in the current study, 17 outer membrane proteins from vibrio parahaemolyticus were identified as immunogens. furt ...201627071519
characterization and functional analysis of a tandem-repeat galectin-9 in large yellow croaker larimichthys crocea.galectins are a family of endogenous lectins with β-galactosides affinity, playing significant roles in the innate immunity of vertebrates and invertebrates. in this report, a new galectin-9 cdna was identified and characterized in large yellow croaker larimichthys crocea (designated as lcgal-9). the complete cdna sequence of lcgal-9 was 1795 bp, with an open reading frame (orf) of 1032 bp encoding 343 amino acids. the putative lcgal-9 protein contained two carbohydrate recognition domains (crds ...201626997199
characterization of blooming algae and bloom-associated changes in the water quality parameters of traditional pokkali cum prawn fields along the south west coast of india.in tropical shrimp farms, especially in traditional pokkali shrimp ponds, poor water quality management can lead to serious threats like heavy algal blooms and frequent disease outbreaks. an investigation regarding the frequent disease outbreaks in selected pokkali shrimp pond adjoining the cochin backwaters has been conducted. water quality parameters were analyzed during the study period. results indicate a considerable fluctuation in the concentration of dissolved oxygen (do) in the study in ...201626850711
a novel c-type lectin, nattectin-like protein, with a wide range of bacterial agglutination activity in large yellow croaker larimichthys crocea.c-type lectins (ctls) are generally recognized as a superfamily of ca(2+)-dependent carbohydrate-binding proteins, which serve as pattern recognition receptors (prrs) in innate immunity of vertebrates. in this study, the molecular characterization and immune roles of a novel ctl from larimichthys crocea (designated as lcntc) were investigated. lcntc is a novel protein that shared 33%-49% homology with other teleosts ctls. the full-length cdna of lcntc was composed of 859 bp with a 465 bp open re ...201626828263
a novel c-type lectin is involved in the innate immunity of macrobrachium nipponense.c-type lectins (ctls) play important roles in invertebrate innate immunity by recognizing and eliminating pathogens. in the present study, a low-density lipoprotein receptor class a (ldla) domain-containing ctl was identified from the oriental river prawn macrobrachium nipponense, designated as mnctldcp1. the full-length cdna of mnctldcp1 was composed of 1462 bp, with a 999-bp orf encoding a 332-aa protein. an ldla and a single c-type lectin-like domain (ctld) were found. the mrna transcripts of ...201626804648
inhibition of pseudomonas aeruginosa exsa dna-binding activity by n-hydroxybenzimidazoles.the pseudomonas aeruginosa type iii secretion system (t3ss) is a primary virulence determinant and a potential target for antivirulence drugs. one candidate target is exsa, a member of the arac family of dna-binding proteins required for expression of the t3ss. a previous study identified small molecules based on an n-hydroxybenzimidazole scaffold that inhibit the dna-binding activity of several arac proteins, including exsa. in this study, we further characterized a panel of n-hydroxybenzimidaz ...201626574012
purification and host specificity of predatory halobacteriovorax isolates from seawater.halobacteriovorax (formerly bacteriovorax) is a small predatory bacterium found in the marine environment and modulates bacterial pathogens in shellfish. four strains of halobacteriovorax originally isolated in vibrio parahaemolyticus o3:k6 host cells were separated from their prey by an enrichment-filtration-dilution technique for specificity testing in other bacteria. this technique was essential, since 0.45-μm filtration alone was unable to remove infectious vibrio minicells, as determined by ...201626590288
the brief case: safe to go back in the water? vibrio parahaemolyticus wound infection associated with brackish water. 201627217385
a novel pcr-based approach for accurate identification of vibrio parahaemolyticus.a pcr-based assay was developed for more accurate identification of vibrio parahaemolyticus through targeting the bla carb-17 like element, an intrinsic β-lactamase gene that may also be regarded as a novel species-specific genetic marker of this organism. homologous analysis showed that bla carb-17 like genes were more conservative than the tlh, toxr and atpa genes, the genetic markers commonly used as detection targets in identification of v. parahaemolyticus. our data showed that this bla car ...201626858713
the new antimicrobial peptide sphyastatin from the mud crab scylla paramamosain with multiple antimicrobial mechanisms and high effect on bacterial infection.sphyastatin was first identified as a new cationic antimicrobial peptide in hemocytes of the mud crab scylla paramamosain. based on the amino acid sequences deduced, it was predicted that this peptide was composed of two different functional domains, a proline-rich domain (prd) and a cysteine-rich domain (crd). the recombinant product of sphyastatin displayed potent antimicrobial activities against the human pathogen staphylococcus aureus and the aquatic animal pathogens aeromonas hydrophila and ...201627493644
development of a quadruplex loop-mediated isothermal amplification assay for field detection of four vibrio species associated with fish disease.a quadruplex loop-mediated isothermal amplification (lamp) method was developed to detect four vibrio species, including vibrio ichthyoenteri, vibrio parahaemolyticus, vibrio scophthalmi, and vibrio vulnificus, simultaneously. four sets of species-specific primers were designed with different restriction sites contained in the inner primers. the quadruplex lamp method could distinguish four vibrio species via the subsequent restriction enzyme analysis. the sensitivity of the quadruplex lamp meth ...201627468405
sensitive visual detection of ahpnd bacteria using loop-mediated isothermal amplification combined with dna-functionalized gold nanoparticles as probes.acute hepatopancreatic necrosis disease (ahpnd) is a component cause of early mortality syndrome (ems) of shrimp. in 2013, the causative agent was found to be unique isolates of vibrio parahaemolyticus (vpahpnd) that contained a 69 kbp plasmid (pap1) carrying binary pir-like toxin genes pirvpa and pirvpb. in thailand, ahpnd was first recognized in 2012, prior to knowledge of the causative agent, and it subsequently led to a precipitous drop in shrimp production. after vpahpnd was characterized, ...201627003504
polar flagella rotation in vibrio parahaemolyticus confers resistance to bacteriophage infection.bacteriophage has been recognized as a novel approach to treat bacterial infectious diseases. however, phage resistance may reduce the efficacy of phage therapy. here, we described a mechanism of bacterial resistance to phage infections. in gram-negative enteric pathogen vibrio parahaemolyticus, we found that polar flagella can reduce the phage infectivity. deletion of polar flagella, but not the lateral flagella, can dramatically promote the adsorption of phage to the bacteria and enhances the ...201627189325
lptd is a promising vaccine antigen and potential immunotherapeutic target for protection against vibrio species infection.outer membrane proteins (omps) are unique to gram-negative bacteria. several features, including surface exposure, conservation among strains and ability to induce immune responses, make omps attractive targets for using as vaccine antigens and immunotherapeutics. lptd is an essential omp that mediates the final transport of lipopolysaccharide (lps) to outer leaflet. the protein in vibrio parahaemolyticus was identified to have immunogenicity in our previous report. in this study, broad distribu ...201627922123
nqrm (duf539) protein is required for maturation of bacterial na+-translocating nadh:quinone oxidoreductase.na(+)-translocating nadh:quinone oxidoreductase (na(+)-nqr) catalyzes electron transfer from nadh to ubiquinone in the bacterial respiratory chain, coupled with na(+) translocation across the membrane. na(+)-nqr maturation involves covalent attachment of flavin mononucleotide (fmn) residues, catalyzed by flavin transferase encoded by the nqr-associated apbe gene. analysis of complete bacterial genomes has revealed another putative gene (duf539, here renamed nqrm) that usually follows the apbe ge ...201626644436
influence of oxyr on growth, biofilm formation, and mobility of vibrio parahaemolyticus.vibrio parahaemolyticus is a common marine food-borne enteropathogen. in this study, we examined the antioxidative activity, growth, biofilm formation, and cell mobility of an oxyr deletion mutant and its genetically complementary strain of v. parahaemolyticus. oxyr is the regulator of catalase and ahpc genes. protection against extrinsic h2o2 and against the organic peroxides cumene hydroperoxide and tert-butyl hydroperoxide was weaker in the deletion mutant than in its parent strain. expressio ...201626590276
techniques in guided group interaction programs. 20165084867
the introduction of a token economy in a psychiatric ward with extremely withdrawn chronic schizophrenics. 20165030250
[studies on the thyroid gland. 3. cholinesterase activity of the blood and serum in patients before and after resection of the goiter]. 20165006160
a double-blind clinical trial with a digestive enzyme product. 20164935696
the development and mature structure of dentine in the pike, esox lucius, analyzed by bright field, phase and polarization microscopy. 20164918183
psychosomatic study and treatment of asthmatic children. 20164885827
[anton musger on his 70th birthday]. 20164880808
introduction of g. robert coatney as charles franklin craig lecturer. 20164869107
editorial: towards medical audit. 20164856376
[statokinesiometry. technic and use]. 20164845332
[esophageal ectasia in the course of achalasia of the cardia]. 20164840284
development of sympathetic uveitis in spite of corticosteroid therapy. 20164835058
"what is a general internist"? 20164832171
ciliogenesis and ciliary anomalies in thymic cysts of "nude" mice. 20164831955
[morphologic picture of senile elastosis surrounding krompecher carcinoma]. 20165035616
[digestive intolerance of cow's milk proteins]. 20164801881
studies in bioluminescence. xii. metal catalysed oxidation of pholad luciferin and of luminol. 20164772295
[problems of sexual psychophysiology and psychopathology in children]. 20164775212
new procedure for training chinchillas for psychoacoustic experiments. 20164754390
impacts of climatic variability on vibrio parahaemolyticus outbreaks in taiwan.this study aimed to investigate and quantify the relationship between climate variation and incidence of vibrio parahaemolyticus in taiwan. specifically, seasonal autoregressive integrated moving average (arima) models (including autoregression, seasonality, and a lag-time effect) were employed to predict the role of climatic factors (including temperature, rainfall, relative humidity, ocean temperature and ocean salinity) on the incidence of v. parahaemolyticus in taiwan between 2000 and 2011. ...201626848675
multiple cross displacement amplification combined with gold nanoparticle-based lateral flow biosensor for detection of vibrio parahaemolyticus.vibrio parahaemolyticus (v. parahaemolyticus) is a marine seafood-borne pathogen causing severe illnesses in humans and aquatic animals. in the present study, multiple cross displacement amplification was combined with a lateral flow biosensor (mcda-lfb) to detect the toxr gene of v. parahaemolyticus in dna extracts from pure cultures and spiked oyster homogenates. amplification was carried out at a constant temperature (62°c) for only 30 min, and amplification products were directly applied to ...201628066368
the 12 gastrointestinal pathogens spectrum of acute infectious diarrhea in a sentinel hospital, shenzhen, china.acute infectious gastroenteritis is one of the most common diseases among all ages, particularly in developing countries. the pathogen spectrum may differ among different regions and seasons. to investigate the etiology of acute diarrhea in shenzhen, a prospective study was conducted from august 2014 to september 2015. stools from 412 patients with diarrhea (286 of whom were adults) including the general epidemiological information of the patients were collected. the 19 pathogens were detected b ...201627965649
colorimetric integrated pcr protocol for rapid detection of vibrio parahaemolyticus.rapid detection of pathogens is of great significance for food safety and disease diagnosis. a new colorimetric method for rapid and easy detection of vibrio parahaemolyticus (v. parahaemolyticus or vp) has been developed in this research. a specific sequence was designed and integrated with the forward primer for molecular detection of vp. this specific sequence was tested and treated as the horseradish peroxidase (hrp)-mimicking dnazyme and could be amplified during the polymerase chain reacti ...201627690041
characteristics of bacterial pathogens associated with acute diarrhea in children under 5 years of age: a hospital-based cross-sectional study.acute diarrhea is a leading cause of morbidity and mortality in children, particularly in those under the age of 5 years. rotavirus is recognized as the leading cause of acute diarrhea in children, however, the contribution of bacterial pathogens as causative agents varies throughout the world. here we report a hospital-based prospective study to analyze the characteristics of bacterial pathogens associated with acute diarrhea in children under 5 years of age.201627267601
host, pathogen and the environment: the case of macrobrachium rosenbergii, vibrio parahaemolyticus and magnesium.macrobrachium rosenbergii is well-known as the giant freshwater prawn, and is a commercially significant source of seafood. its production can be affected by various bacterial contaminations. among which, the genus vibrio shows a higher prevalence in aquatic organisms, especially m. rosenbergii, causing food-borne illnesses. vibrio parahaemolyticus, a species of vibrio is reported as the main causative of the early mortality syndrome. vibrio parahaemolyticus infection in m. rosenbergii was studi ...201627114742
genomic features of environmental and clinical vibrio parahaemolyticus isolates lacking recognized virulence factors are dissimilar.vibrio parahaemolyticus is a bacterial pathogen that can cause illness after the consumption or handling of contaminated seafood. the primary virulence factors associated with v. parahaemolyticus illness are thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh). however, clinical strains lacking tdh and trh have recently been isolated, and these clinical isolates are poorly understood. to help understand the emergence of clinical tdh- and trh-negative isolates, a genomic approach w ...201626637607
the autonomic nervous system and the peripheral circulation. 20164911050
[racemization during peptide synthesis]. 20164881545
[double electron-nuclear resonance of free radicals in gamma-irradiated glycine single crystals]. 20164701432
is jump to population-explosion bandwagon justified? 20165009981
[early postoperative rehabilitation after maxillary resection]. 20164951118
[measurement of central venous pressure: only in recumbent position?]. 20164829023
[subphrenic abscess and its x-ray differential diagnosis]. 20165076712
[circumstantial study of accidents in minors under 15 years of age (study of 100 cases)]. 20164791562
[the bioelectrical activity of the muscles in patients following poliomyelitis]. 20164775888
primary angle-closure glaucoma in urbanized south african caucasoid and negroid communities. 20164725852
[examinations on the influence of arterial blood pressure on the course of glaucoma simplex]. 20165095602
[successfully treated case of traumatic ventricular septal perforation caused by stabbing]. 20165090175
contrast laryngography in diagnosis. 20165067765
[late results of surgery on the thyroid gland]. 20165068155
simultaneous dispersion and decay of radioactive wastes. 20165014874
are you receiving me? 20165014404
[chromatoclastic and mitoclastic activity of some fractions of tars of cigarette smoke]. 20164987574
rejection of the generic name dendrocystis rees, 1962, and its replacement by arborocystis nov.nom. protozoa: gregarinomorpha. 20164960245
[transduodenal papillotomy and sphincterotomy in biliary surgery: general review of 132 cases]. 20164913238
[death of dr. guillermo mann fischer]. 20164877508
subacute sclerosing encephalitis. 20164862504
[letter: homologous insemination of poole sperma]. 20164849080
[our equipment for cortical audiometry]. 20164837373
letter: how to stamp out creativity. 20164820304
environmental lead pollution. 20164821235
letter: on lid loading in the management of lagophthalmos. 20164843124
letter: beyond cost estimating, another ball game. 20164820302
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