Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| histidine decarboxylases and their role in accumulation of histamine in tuna and dried saury. | histamine-producing bacteria (hpb) such as photobacterium phosphoreum and raoultella planticola possess histidine decarboxylase (hdc), which converts histidine into histamine. histamine fish poisoning (hfp) is attributable to the ingestion of fish containing high levels of histamine produced by hpb. because freezing greatly decreases the histamine-producing ability of hpb, especially of p. phosphoreum, it has been speculated that hfp is caused by hdc itself from hpb cells autolyzing during froze ... | 2007 | 17220267 |
| effect of heavy atoms in bioluminescent reactions. | bioluminescent reactions of luminous organisms are excellent models for studying the effects of heavy atoms on enzymatic processes. the effects of potassium halides with halide anions of different atomic weight were compared in bioluminescent reactions of the firefly (luciola mingrelica), a marine coelenterate (obelia longissima), and a marine bacterium (photobacterium leiognathi). two mechanisms of the effects of the halides were examined-the physicochemical effect of the external heavy atom, b ... | 2007 | 17237922 |
| cytochemical and ultrastructural study of anoikis and secondary necrosis in enterocytes detached in vivo. | detachment-induced apoptosis of enterocytes (anoikis) has not been investigated in vivo. here we describe anoikis of fish enterocytes following detachment in a septicemia by photobacterium damselae subsp. piscicida, or following injection of its exotoxin. the in vivo study was complemented with an ex vivo time-lapse analysis using conditions duplicating the in vivo situation. linings of enterocytes detached from intestine mucosa dissociate into isolated enterocytes which undergo caspase 3-mediat ... | 2007 | 17245641 |
| photobacterium lutimaris sp. nov., isolated from a tidal flat sediment in korea. | a gram-negative, motile, pale-yellow-pigmented, oval-shaped bacterial strain, df-42t, was isolated from a tidal flat sediment in korea. strain df-42t grew optimally at 25-30 degrees c and in the presence of 2-3 % (w/v) nacl. it contained q-8 as the predominant ubiquinone and c(16 : 0), c(18 : 1)omega7c and summed feature 3 (c(16 : 1)omega7c and/or iso-c(15 : 0) 2-oh) as the major fatty acids. the dna g+c content was 48.3 mol%. phylogenetic trees based on 16s rrna gene sequences showed that strai ... | 2007 | 17267974 |
| mechanistic evidence and efficiency of the cr(vi) reduction in water by different sources of zerovalent irons. | the objective of this research was to assess the mechanism and effectiveness of the zerovalent iron (zvi) to remediate the cr(vi) contaminated water. the mechanism of cr(vi) reduction by zvi was evaluated by characterising surface properties and chemical compositions of fe and cr products using sem-eds, xrd and xps analyses. the effectiveness of zvi in cr(vi) reduction was assessed by the luminescent bacteria (photobacterium phosphoreum). the cr(vi) was reduced to cr(iii), when the reactive fe(o ... | 2007 | 17305140 |
| [toxicity reduction of synthetic municipal wastewater treated by anaerobic, anoxic or aerobic reactors]. | bioassay with photobacterium phosphoreum was used to evaluate the toxicity of municipal wastewater. the toxicity reduction capabilities of three biological treatment processes (anaerobic reactor, anoxic reactor and aerobic reactor) treating the synthetic municipal wastewater were studied. seven compounds including toluene, p-xylene, o-xylene, indole, pyridine, cyclohexanone and benzenepropanoic acid were selected to represent the main organic toxicants in the municipal wastewater. the gc-ms anal ... | 2006 | 17326447 |
| differential annotation of trna genes with anticodon cat in bacterial genomes. | we have developed three strategies to discriminate among the three types of trna genes with anticodon cat (trna(ile), elongator trna(met) and initiator trna(fmet)) in bacterial genomes. with these strategies, we have classified the trna genes from 234 bacterial and several organellar genomes. these sequences, in an aligned or unaligned format, may be used for the identification and annotation of trna (cat) genes in other genomes. the first strategy is based on the position of the problem sequenc ... | 2006 | 17071718 |
| a siderophore biosynthesis gene cluster from the fish pathogen photobacterium damselae subsp. piscicida is structurally and functionally related to the yersinia high-pathogenicity island. | photobacterium damselae subsp. piscicida, the causative agent of fish pasteurellosis, produces a siderophore which is distinct from that produced by p. damselae subsp. damselae. using suppression subtractive hybridization, a subsp. piscicida-specific dna region of 35 kb was identified in strain di21, and 11 genes were defined: dahp, arac1, arac2, frpa, irp8, irp2, irp1, irp3, irp4, irp9 and irp5. the sequence of the predicted proteins encoded by these genes showed significant similarity with the ... | 2006 | 17074903 |
| [joint toxicity on multi-component mixtures of sds and substituted aromatic compounds]. | the toxicity to photobacterium phosphoreum of different mixture systems of sodium dodecyl sulfate (sds) and three substituted aromatic compounds according to equiconcentration ratio of 1 : 1, as well as individual toxicity, were determined respectively. the joint toxicity was evaluated by toxicity unit (tu), additive index (ai), similarity parameter (lambda), and mixtures toxicity index (mti). four evaluating methods were compared and the modes of joint action of different systems were analyzed ... | 2006 | 17111627 |
| toxicity assessment of contaminated sediments after dredging. | 2006 | 17219312 | |
| photolyase confers resistance to uv light but does not contribute to the symbiotic benefit of bioluminescence in vibrio fischeri es114. | recent reports suggest that the selective advantage of bioluminescence for bacteria is mediated by light-dependent stimulation of photolyase to repair dna lesions. despite evidence for this model, photolyase mutants have not been characterized in a naturally bioluminescent bacterium, nor has this hypothesis been tested in bioluminescent bacteria under natural conditions. we have now characterized the photolyase encoded by phr in the bioluminescent bacterium vibrio fischeri es114. consistent with ... | 2006 | 17021211 |
| influence of storage temperature on microbial spoilage characteristics of haddock fillets (melanogrammus aeglefinus) evaluated by multivariate quality prediction. | the proliferation of specific spoilage organisms (sso) and quality changes were evaluated in haddock fillets stored in styrofoam boxes at 0, 7 and 15 degrees c and under temperature fluctuations. a rapid electronic nose technique was used to monitor different classes of compounds, representing microbial metabolites that were characteristic for the onset of spoilage odors. photobacterium phosphoreum predominated among the spoilage bacteria and high levels of tvb-n were observed at sensory rejecti ... | 2006 | 16889858 |
| cloning and sequencing of the histidine decarboxylase gene from photobacterium phosphoreum and its functional expression in escherichia coli. | the major causative agent of scombroid poisoning is histamine formed by bacterial decarboxylation of histidine. we reported previously that histamine was exclusively formed by the psychrotrophic halophilic bacteria photobacterium phosphoreum in scombroid fish during storage at or below 10 degrees c. moreover, histamine-forming ability was affected by two histidine decarboxylases (hdcs): constitutive and inducible enzymes. in this study, the gene encoding p. phosphoreum hdc was cloned into escher ... | 2006 | 16924898 |
| stability of chloramphenicol residues in shrimp subjected to heat processing treatments. | the stability of chloramphenicol residues in white shrimp (penaeus indicus) subjected to cooking (100 degrees c) for 10, 20 and 30 min (c1, c2 and c3) as well as retorting (121 degrees c) for 10 and 15 min (r1 and r2) was studied by a microbial assay method using photobacterium leiognathi as the test organism. the microbial assay method was found to have a good sensitivity of 1 microg/ml the loss of chloramphenicol in shrimp subjected to cooking for 10, 20 and 30 min was 6%, 12% and 29%, respect ... | 2006 | 16942985 |
| ompw and ompv are required for nacl regulation in photobacterium damsela. | photobacterium damsela is a marine pathogen to both fish and human beings. the bacterium can shift between the ambient seawater and hosts, suggesting the existence of proteins rapidly responding to salt concentration. in the current study, proteomic methodologies were applied to screen the outer membrane proteins (omps) related to salt stress. ompw and ompv were determined in the response in this bacterium as ompc and ompf did in e. coli. furthermore, the two genes were overexpressed in e. coli ... | 2006 | 16944937 |
| effect of bacterial interactions on the spoilage of cold-smoked salmon. | cold-smoked salmon is a lightly preserved fish product in which a mixed microbial flora develops during storage and where the interactive behaviour of micro-organisms may contribute to their growth and spoilage activity. the aim of this study was to assess the effect of the bacterial interactions between the main species contaminating the cold-smoked salmon on bacterial growth, chemical and sensory changes, and spoilage. first, carnobacterium piscicola, photobacterium phosphoreum, lactobacillus ... | 2006 | 16949172 |
| control of the bioluminescence starting time by inoculated cell density. | in this study, we attempted to control the timing of light-emission from bioluminescent bacteria, by changed cell numbers inoculated into medium. luminous bacteria express bioluminescence when the number of cells reached a threshold. inoculated cell density had an effect on the time of bioluminescence starting. samples were prepared by varying cell density of inoculation. in the results, all the vials showed different luminescence profiles in the order of inoculated cell population. | 2006 | 16966816 |
| eliminating toxic compounds by composting olive mill wastewater-straw mixtures. | the present work studies the changes occurring in organic matter, phenols and biotoxicity on composting olive mill wastewater with barley straw. the total organic matter decreased, a drop of 25% was reached after the stabilization phase and 52% at the end of the maturation phase. degradation of the phenols reached 54% and 95%, respectively, after these periods. the toxicity of the water extract, evaluated by the photobacterium phosphoreum fluorescence, decreased to disappear after only 2 months ... | 2006 | 16973264 |
| [heterogeneity of the populations of marine luminescent bacteria photobacterium leiognathi under different conditions of cultivation]. | manifestation of pleiotropic effects in the isogenic variants of luminescent bacteria photobacterium leiognathi 54 was investigated. the decrease or increase of the expression level of bioluminescence was caused by changes in lux operon regulation. the dynamics of the bioluminescence of dark and dim variants did not differ from the dynamics of the initial luminescent variant, but dependence of the level of luminescence intensity on the exogenous autoinductor of the lux operon was revealed. the i ... | 2006 | 16871801 |
| spinal deformity in a sandtiger shark, carcharias taurus rafinesque: a clinical-pathological study. | the present study investigated spinal deformity in a captive sandtiger shark and describes the clinical and histopathological features of the lesion. clinically, the shark presented a marked spinal curvature in the region between the pectoral girdle and cranial dorsal fin. radiographic studies revealed scoliosis and kyphosis of the vertebral column with a modification of the basic structure of five vertebrae. post-mortem examination confirmed the results of radiographic studies and histological ... | 2006 | 16351698 |
| photobacterium ganghwense sp. nov., a halophilic bacterium isolated from sea water. | a marine bacterial strain, designated fr1311t, was isolated from a sea-water sample from ganghwa island, south korea. cells were gram-negative, facultatively anaerobic, catalase- and oxidase-positive, motile, oval or rod-shaped and halophilic (optimum sea-salt concentration for growth of 5-6 %). phylogenetic analysis of its 16s rrna gene sequence revealed that it represented a distinct line of descent within the genus photobacterium. the major fatty acids were straight-chain saturated (c(16 : 0) ... | 2006 | 16585687 |
| piezophilic adaptation: a genomic point of view. | two-thirds of earth's surface is covered by oceans, yet the study of this massive integrated living system is still in its infancy. various environmental variables, such as high salinity, low and changeable nutrient availability and depth-correlated gradients of light, temperature, nutrients and pressure shape the diversity, physiology and ecology of marine species. as oceans present an average depth of 3800 m, deep-sea ecosystems represent the most common marine ecological niche. one of the key ... | 2006 | 16780980 |
| larval settlement of the common australian sea urchin heliocidaris erythrogramma in response to bacteria from the surface of coralline algae. | bacterial biofilms are increasingly seen as important for the successful settlement of marine invertebrate larvae. here we tested the effects of biofilms on settlement of the sea urchin heliocidaris erythrogramma. larvae settled on many surfaces including various algal species, rocks, sand and shells. settlement was reduced by autoclaving rocks and algae, and by treatment of algae with antibiotics. these results, and molecular and culture-based analyses, suggested that the bacterial community on ... | 2006 | 16794830 |
| cloning and sequencing analysis of alginate lyase genes from the marine bacterium vibrio sp. o2. | we isolated a new marine bacteria, which displayed alginate-depolymerizing activity in plate assays, from seawater in mihonoseki harbor, japan. analysis of the 16s ribosomal rna gene sequence of one of the isolates proved that this alginate-depolymerizing bacterium belonged to the genus vibrio and it was named vibrio sp. o2. the alginate lyase genes of vibrio sp. o2 were cloned and expressed in escherichia coli. two alginate lyase-producing clones, pvoa-a4 and pvoa-b5, were obtained. the alginat ... | 2006 | 16810458 |
| biogenic amine formation and microbial spoilage in chilled garfish (belone belone belone)--effect of modified atmosphere packaging and previous frozen storage. | to evaluate biogenic amine formation and microbial spoilage in fresh and thawed chilled garfish. | 2006 | 16834594 |
| bioavailability and toxicity of heavy metals in the presence of natural organic matter. | the effect of humic acids (ha) on the toxicity of copper, zinc and lead was investigated in this study, using the photobacterium vibrio fischeri (microtox test) as a test organism. the metal species and the complexation capacity of ha were predicted using model winhumicv for speciation modeling. the toxicities of copper and lead were generally comparable, while the toxicity of zinc was lower than the ones of the other two method. the toxicity of copper decreased with the addition of ha, while th ... | 2006 | 16835107 |
| application of leaching tests for toxicity evaluation of coal fly ash. | the toxic properties of coal fly ash samples obtained from various coal combustion power plants were evaluated in this work using physicochemical analyses and bioassays. physicochemical analyses showed that heavy metals present in solid samples included cr, cu, mn, ni, pb, and zn. the results of the chemical analysis of eluates deduced by the application of standard leaching tests according to en 12457-2 and toxicity characteristic leaching procedure (tclp) methods indicated that the compounds c ... | 2006 | 16841327 |
| luxa gene of light organ symbionts of the bioluminescent fish acropoma japonicum (acropomatidae) and siphamia versicolor (apogonidae) forms a lineage closely related to that of photobacterium leiognathi ssp. mandapamensis. | a molecular phylogenetic analysis of luxa gene sequences of light organ symbionts of the fish acropoma japonicum (acropomatidae) and siphamia versicolor (apogonidae) revealed that the sequences were related to those of photobacterium leiognathi ssp. mandapamensis, which is not known to occur as a light organ symbiont among bioluminescent p. leiognathi clades. the presence of another lux gene element, luxf, coding for nonfluorescent protein, provided additional support for the identity of the lig ... | 2006 | 16842343 |
| dual function of fish hepcidin: response to experimental iron overload and bacterial infection in sea bass (dicentrarchus labrax). | the role of hepcidin in iron metabolism regulation and bacterial infection has been the focus of recent attention. however, in spite of the growing number of hepcidin genes known from different organisms, little is known about its putative dual function in fish. the aim of this study was to characterize the sea bass hepcidin gene and to study its role in iron metabolism and infection. the novel sea bass hepcidin gene was found to be organized into two introns and three exons with several copies ... | 2006 | 16616368 |
| photobacterium halotolerans sp. nov., isolated from lake martel in spain. | a halotolerant bacterium was isolated from a saline lake located in mallorca, spain. cells of the strain, designated macl01t, were gram-negative, rod-shaped and motile by means of polar flagella. colonies of strain macl01t were white to cream in tsa medium, turning brown after 7 days of incubation; they were blue in thiosulphate/citrate/bile salts/sucrose agar medium. a neighbour-joining phylogenetic analysis based on 16s rrna gene sequences showed that strain macl01t belongs to the genus photob ... | 2006 | 16627656 |
| formation of histamine and biogenic amines in cold-smoked tuna: an investigation of psychrotolerant bacteria from samples implicated in cases of histamine fish poisoning. | two outbreaks and a single case of histamine fish poisoning associated with cold-smoked tuna (cst) were reported in denmark during 2004. the bacteria most likely responsible for histamine formation in cst implicated in histamine fish poisoning was identified for the first time in this study. product characteristics and profiles of biogenic amines in the implicated products were also recorded. in the single poisoning case, psychrotolerant morganella morganii-like bacteria most likely was responsi ... | 2006 | 16629036 |
| aldolase-catalyzed synthesis of beta-d-galp-(1-->9)-d-kdn: a novel acceptor for sialyltransferases. | [reaction: see text] beta-d-galp-(1-->9)-d-kdn, a disaccharide component of the cell wall of streptomyces sp. mb-8, was synthesized from beta-d-galp-(1-->6)-d-manp and pyruvate using a sialic acid aldolase. the obtained kdn-containing compound was a novel acceptor for bacterial sialyltransferases. unusual alpha2,3- and alpha2,6-linked sialyltrisaccharides and a tetrasaccharide were synthesized using a one-pot two-enzyme system containing a neisseria meningitidis cmp-sialic acid synthetase and a ... | 2006 | 16706534 |
| drug resistance and random amplified polymorphic dna analysis of photobacterium damselae ssp. piscicida isolates from cultured seriola (yellowtail, amberjack and kingfish) in japan. | to investigate the antimicrobial susceptibility and genetic characteristics of photobacterium damselae ssp. piscicida isolates obtained from cultured seriola in japan. | 2006 | 16706907 |
| highly efficient chemoenzymatic synthesis of naturally occurring and non-natural alpha-2,6-linked sialosides: a p. damsela alpha-2,6-sialyltransferase with extremely flexible donor-substrate specificity. | 2006 | 16721893 | |
| infection with photobacterium damselae subspecies damselae and vibrio harveyi in snapper, pagrus auratus with bloat. | to diagnose the cause of chronic, low mortality associated with bloat in tanks of snapper at an aquaculture facility. | 2006 | 16739527 |
| [proteolytic activity of luminous bacteria]. | 2006 | 16758880 | |
| superoxide dismutase and catalase activities in photobacterium damselae ssp. piscicida. | the ability of a set of photobacterium damselae ssp. piscicida strains isolated from different fish species to produce different superoxide dismutase (sod) and catalase enzymes was determined. unlike other bacterial pathogens, p. damselae ssp. piscicida is not able to produce different isoforms of sod or catalase containing different metal cofactors when cultured under oxidative stress induced by hydrogen peroxide or methyl viologen, or under iron depleted conditions. however, iron content of th ... | 2006 | 16768716 |
| effect of salt stress on pigment production of serratia rubidaea n-1: a potential indicator strain for screening quorum sensing inhibitors from marine microbes. | 2006 | 16778355 | |
| complement consumption by photobacterium damselae subsp. piscicida in seabream, red porgy and seabass normal and immune serum. effect of the capsule on the bactericidal effect. | a virulent strain of photobacterium damselae subsp. piscicida (pdp) was grown without (c form) or with (c+ form) glucose supplementation, the latter to enhance capsule formation. both forms were resistant to killing by normal serum of seabream, red porgy and seabass. however, the c form was killed by immune serum of all three fish species while the c+ form was killed only by seabream and red porgy sera and to a lesser extent than the c form. both c and c+ forms consumed complement in normal seru ... | 2006 | 16263309 |
| oxidation of resin and fatty acids by ozone: kinetics and toxicity study. | the ozonation of resin and fatty acids (rfas) found in pulp mill effluents was investigated using rapid-scan stopped-flow spectrophotometry. rfas oxidation (i.e., degradation) efficiency increased with increasing the amount of used ozone and temperature. the degradation process with respect to the acid was found to follow first-order kinetics. the ozonation of rfas was modeled as an overall second-order reaction for both reactants. the apparent overall second-order rate constants were calculated ... | 2006 | 16376965 |
| bioluminescence and exogenous compounds: physico-chemical basis for bioluminescent assay. | bioluminescent systems are convenient objects to study mechanisms of influence of exogenous molecules on living organisms. classification of physical and physico-chemical mechanisms of the effects of luminous bacteria photobacterium leiognathi on bioluminescent reactions is suggested. five mechanisms are discussed: (1) change of electron-excited states' population and energy transfer, (2) change of efficiency of s-t conversion in the presence of external heavy atom, (3) change of rates of couple ... | 2006 | 16413195 |
| the sos-lux-toxicity-test on the international space station. | for the safety of astronauts and to ensure the stability and integrity of the genome of microorganisms and plants used in bioregenerative life support systems, it is important to improve our knowledge of the combined action of (space) radiation and microgravity. the sos-lux-toxicity test, as part of the triple-lux project (accepted for flight at biolab in columbus on the international space station, (iss)), will provide an estimation of the health risk resulting from exposure of astronauts to th ... | 2006 | 16431084 |
| effects of chlorine disinfection on toxicity formation in reclaimed water. | 2006 | 16468000 | |
| rapid identification of marine bioluminescent bacteria by amplified 16s ribosomal rna gene restriction analysis. | to rapidly identify natural isolates of marine bioluminescent bacteria, we developed amplified ribosomal dna restriction analysis (ardra) methods. ardra, which is based on the restriction patterns of 16s rrna gene digested with five enzymes (ecori, ddei, hhai, hinfi, rsai), clearly distinguished the 14 species of marine bioluminescent bacteria currently known, which belong to the genera vibrio, photobacterium, and shewanella. when we applied ardra to 129 natural isolates from two cruises in saga ... | 2006 | 16499620 |
| first isolation of photobacterium damselae ssp. damselae from cultured redbanded seabream, pagrus auriga valenciennes, in spain. | 2006 | 16533303 | |
| interactive toxic effects of heavy metals and humic acids on vibrio fischeri. | the effect of humic acids (has) on the toxicity of copper, zinc, and lead was investigated using the photobacterium vibrio fischeri (microtox test) as a test organism. the effects of has on metal toxicity were evaluated as functions of time and concentration in pure compound solutions. the toxicities of copper and lead were generally comparable, while the toxicity of zinc was lower than those of the other two metals. the toxicity of copper decreased with the addition of has, while the toxicity o ... | 2006 | 15939470 |
| coagulant toxicity and effectiveness in a slaughterhouse wastewater treatment plant. | attempts were made in this study to examine the toxicity of polymer/alum addition to the aeration tank effluent prior to sludge flotation as practiced in a slaughterhouse wastewater treatment plant. based on the microtox toxicity assay, alum at concentrations 100-200 mg/l was found to slightly increase the toxicity level of slaughterhouse wastewater effluent. however, at higher concentrations (300-1000 mg/l), significant residual chronic toxicity remained in all slaughterhouse wastewater effluen ... | 2006 | 16040124 |
| new cold-adapted lipase from photobacterium lipolyticum sp. nov. that is closely related to filamentous fungal lipases. | a photobacterium strain, m37, showing lipolytic activity, was previously isolated from an intertidal flat of the yellow sea in korea and identified as photobacterium lipolyticum sp. nov. in the present study, the corresponding gene was cloned using the shotgun method. the amino acid sequence deduced from the nucleotide sequence (1,023 bp) corresponded to a protein of 340 amino acid residues with a molecular weight of 38,026. no sequence similarity was found with any known bacterial lipases/ester ... | 2006 | 16088345 |
| toxicity measurements in aqueous solution during ozonation of mono-chlorophenols. | the microtox toxicity and oxygen uptake rate (our) inhibition tests were conducted to monitor the variation of toxicity during ozonation of 2-chlorophenol (2-cp), 3-chlorophenol (3-cp) and 4-chlorophenol (4-cp) under neutral conditions. the results revealed that the oxidized 2-cp solution exhibited new toxicity to pure bacteria and mixed microorganisms in the early stage of ozonation. the largest inhibition of our appeared at one mol of applied ozone dosage per mol of initial 2-cp, and the perce ... | 2006 | 16122866 |
| purification and characterization of recombinant caulobacter crescentus cu,zn superoxide dismutase. | recombinant cu,zn superoxide dismutase from caulobacter crescentus has been expressed in escherichia coli and characterized. the corresponding recombinant protein has a molecular weight typical of a homodimeric cu,znsods and an activity comparable to that of other prokaryotic enzymes. the copper active site is characterized by a peculiar axial geometry as evidenced by its electron paramagnetic resonance spectrum, moreover, the copper atom displays a low accessibility toward external chelating ag ... | 2006 | 16213200 |
| effect of salinity on the immune response of tiger shrimp penaeus monodon and its susceptibility to photobacterium damselae subsp. damselae. | addition of nacl at 2.5% to tryptic soy broth (tsb) significantly increased the growth of photobacterium damselae subsp. damselae. tiger shrimp penaeus monodon held in 25 per thousand seawater were injected with p. damsela subsp. damselae grown in tsb containing nacl at 0.5%, 1.5%, 2.5% and 3.5% at a dose of 8.48 x 10(4)colony-forming units (cfu)shrimp(-1). over 24-96 h, the cumulative mortality was significantly higher for the shrimp challenged with p. damselae subsp. damselae grown in 2.5% nac ... | 2006 | 16213751 |
| an unusual case of urinary tract infection in a pregnant woman with photobacterium damsela. | we describe a case of a urinary tract infection with an unusual pathogen, photobacterium damsela, in a pregnant female. this pathogen has been described as having a virulent life threatening nature, so a detailed history and prompt treatment is needed. | 2006 | 17485811 |
| one-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities. | chemoenzymatic synthesis, which combines the flexibility of chemical synthesis and the high selectivity of enzymatic synthesis, is a powerful approach to obtain complex carbohydrates. it is a preferred method for synthesizing sialic acid-containing structures, including those with diverse naturally occurring and non-natural sialic acid forms, different sialyl linkages and different glycans that link to the sialic acid. starting from n-acetylmannosamine, mannose or their chemically or enzymatical ... | 2006 | 17406495 |
| [obtaining of bacterial bioluminescent strain protobacterium phosphoreum b7071 (lux+) for the determination of zinc ion concentration]. | the transconjugate containing hybrid plasmid (te(r)zn(r)lux+) was obtained using the conjugation method on pseudomonasfragi t2(5) (te(r)znr) strain and bioluminescent strain protobacterium phosphoreum b7071 (lux+). the expression regulation of lux-genes on the obtained plasmid is carried out by promotor-operational area conjointly with zinc resistance genes. the cells of the obtained genetically modified bacteria have the ability to specific induced luminescence, which is a respond to zinc ions' ... | 2006 | 17147278 |
| on-line determination of serum bactericidal activity using recombinant luminescent bacteria. | intensity of luminescence quenching in recombinant strains of escherichia coli with cloned lux-operones by human blood serum is directly proportional to the degree of bactericidal effect assessed by nephelometric and bacteriological methods. this correlation was most characteristic of e. coli with luminescence genes from photobacterium leiognathi, which substantiates its use in the development of the kinetic bioluminescent method to determine of serum bactericidal activity. the possibility of us ... | 2006 | 17369948 |
| vaccination trials of sea bass (dicentrarchus labrax) against pasteurellosis using oral, intraperitoneal and immersion methods. | photobacterium damsela subsp. piscicida (phdp) is the aetiological agent of fish pasteurellosis, causing heavy losses in intensive mariculture plants. the present work compares the protective efficacy of five different vaccine formulation: oral, intraperitoneal, immersion, bivalent immersion (vibrio anguillarum) and immersion associated with immunostimulants. each of these vaccine formulations containing whole cells of phdp formalin inactivated (fkc), was administered to 100 sea bass weighing ap ... | 2005 | 20437375 |
| vibrionaceae as a possible source of qnr-like quinolone resistance determinants. | to gain insight into the functionality of qnr-like genes of several bacterial species of vibrionaceae that may encode quinolone resistance determinants. | 2005 | 16227349 |
| detoxication of solutions of organic oxidants by humic substances: bioluminescence monitoring. | 2005 | 16229147 | |
| phylogeny and molecular identification of vibrios on the basis of multilocus sequence analysis. | we analyzed the usefulness of rpoa, reca, and pyrh gene sequences for the identification of vibrios. we sequenced fragments of these loci from a collection of 208 representative strains, including 192 well-documented vibrionaceae strains and 16 presumptive vibrio isolates associated with coral bleaching. in order to determine the intraspecies variation among the three loci, we included several representative strains per species. the phylogenetic trees constructed with the different genetic loci ... | 2005 | 16151093 |
| phylogenetic resolution and habitat specificity of members of the photobacterium phosphoreum species group. | substantial ambiguity exists regarding the phylogenetic status of facultatively psychrophilic luminous bacteria identified as photobacterium phosphoreum, a species thought to be widely distributed in the world's oceans and believed to be the specific bioluminescent light-organ symbiont of several deep-sea fishes. members of the p. phosphoreum species group include luminous and non-luminous strains identified phenotypically from a variety of different habitats as well as phylogenetically defined ... | 2005 | 16156737 |
| detection of quinolone-resistance genes in photobacterium damselae subsp. piscicida strains by targeting-induced local lesions in genomes. | quinolone-resistant strains of the fish-pathogenic bacterium, photobacterium damselae subsp. piscicida are distributed widely in cultured yellowtail, seriola quinqueradiata (temminck & schlegel), in japan. the quinolone resistance-determining region (qrdr) was amplified with degenerate primers, followed by cassette ligation-mediated pcr. open reading frames encoding proteins of 875 and 755 amino acid residues were detected in the gyra and parc genes, respectively. resistant strains of p. damsela ... | 2005 | 16159364 |
| laterally transferred elements and high pressure adaptation in photobacterium profundum strains. | oceans cover approximately 70% of the earth's surface with an average depth of 3800 m and a pressure of 38 mpa, thus a large part of the biosphere is occupied by high pressure environments. piezophilic (pressure-loving) organisms are adapted to deep-sea life and grow optimally at pressures higher than 0.1 mpa. to better understand high pressure adaptation from a genomic point of view three different photobacterium profundum strains were compared. using the sequenced piezophile p. profundum strai ... | 2005 | 16162277 |
| pathogenicity of vibrios to rainbow trout (oncorhynchus mykiss, walbaum) and artemia nauplii. | the taxonomy of marine vibrios has changed rapidly over the last two decades, and a wealth of new species have been identified. many vibrio species are pathogenic to fish and crustaceans; however, little is known about the virulence of many of the novel species. the purpose of this study was to determine the ability of various recent isolates of vibrios to cause disease in rainbow trout (oncorhynchus mykiss, walbaum) and crustacea, i.e. artemia nauplii. of 56 isolates, representing 26 species of ... | 2005 | 16104871 |
| experimental and simulative dissociation of dimeric cu,zn superoxide dismutase doubly mutated at the intersubunit surface. | the equilibrium properties of dimeric photobacterium leiognathi cu,zn superoxide dismutase mutant bearing two negative charges in the amino acid clusters at the association interface has been studied, experimentally and computationally, and compared to those of the native enzyme. pressure-dependent dissociation is observed for the mutant, as observed by the fluorescence shift of the unique tryptophan residue located at the intersubunit surface. the spectral shift occurs slowly, reaching a platea ... | 2005 | 15681652 |
| bioluminescence-mediated stimulation of photoreactivation in bacteria. | although biochemistry and genetics of light emission by cells have been investigated in detail, a biological role for bacterial luminescence has remained obscure for a long time. it was proposed recently that luminescence may stimulate dna repair, but the specific mechanism of this phenomenon was not investigated. moreover, experiments showing decreased survival of uv-irradiated lux mutants relative to luminescent cells were performed previously using only one bacterial species, vibrio harveyi. ... | 2005 | 16040205 |
| cloning and characterization of the rpoe gene encoding an rna polymerase sigmae factor from the deep-sea piezophilic shewanella violacea strain dss12. | the rpoe gene encoding an rna polymerase sigmae subunit was isolated from a gamma-phage library of the deep-sea piezophilic and psychrophilic bacterium shewanella violacea strain dss12. structual analysis showed that the gene organization of the fragment containing s. violacearpoe was the l-aspartate oxidase-coding gene, rpoe, rsea, rseb and rsec in that order, the same as in the case of photobacterium profundum ss9 and escherichia coli k-12. the cloned gene, 576 bp in length, was found to encod ... | 2005 | 16040350 |
| quorum sensing signal molecules (acylated homoserine lactones) in gram-negative fish pathogenic bacteria. | the aim of the present study was to investigate the production of quorum sensing signals (specifically acylated homoserine lactones, ahls) among a selection of strains of gram-negative fish bacterial pathogens. these signals are involved in the regulation of virulence factors in some human and plant-pathogenic bacteria. a total of 59 strains, representing 9 different fish pathogenic species, were tested against 2 ahl monitor bacteria (agrobacterium tumefaciens nt1 [pzlr4] and chromobacterium vio ... | 2005 | 16042042 |
| direct identification of photobacterium damselae subspecies piscicida by pcr-rflp analysis. | fish pasteurellosis is an infectious disease that affects several teleost species living in temperate marine waters. the pathogen responsible, photobacterium damselae subspecies piscicida, shows high genetic similarity with p. damselae subsp. damselae, making subspecies discrimination extremely laborious. here we report for the first time a pcr-rflp method for the identification of p. damselae subsp. piscicida without prior isolation in pure culture. genomic sequence information was obtained thr ... | 2005 | 16042043 |
| biochemical characterization of a cambialistic superoxide dismutase isozyme from diatom thallassiosira weissflogii: cloning, expression, and enzyme stability. | a cdna clone of 1081 bp encoding a second putative superoxide dismutase (sod) from diatom thallassiosira weissflogii was cloned by the polymerase chain reaction technique. the cdna encodes a protein of 286 amino acid residues. alignment of the truncated sod sequence containing 217 amino acid residues with mn-sods from vibrio mimicus and escherichia coli, as well as two fe-sods from e. coli and photobacterium leiognathi, this sod showed greater homology to mn-sod. the residues required to coordin ... | 2005 | 16076113 |
| subtractive hybridization reveals a high genetic diversity in the fish pathogen photobacterium damselae subsp. piscicida: evidence of a sxt-like element. | photobacterium damselae subsp. piscicida is the causative agent of fish pasteurellosis, a severe disease affecting cultured marine fish worldwide. in this study, suppression subtractive hybridization was used to identify dna fragments present in the virulent strain pc554.2, but absent in the avirulent strain epoy 8803-ii. twenty-one genomic regions of this type (that included twenty-six distinct putative orfs) were analysed by dna sequencing. twenty orfs encoded proteins with homology to protein ... | 2005 | 16079344 |
| conjugal vectors for cloning, expression, and insertional mutagenesis in gram-negative bacteria. | 2005 | 15945369 | |
| bacterial vaccines for fish--an update of the current situation worldwide. | during the last few years, the use of vaccines for disease prevention in aquaculture has expanded both with regard to the number of fish species and number of microbial diseases. according to the responses to a questionnaire received from 41 countries, vaccination is used in the commercial aquaculture of species like atlantic salmon (salmo salar), rainbow trout (oncorhynchus mykiss), sea bass (dicentrarchus labrax), sea bream (sparus aurata), barramundi (lates calcarifer), tilapia (tilapia spp), ... | 2005 | 15962470 |
| update on bacterial vaccines: photobacterium damselae subsp. piscicida. | photobacterium damselae subsp. piscicida is the causative agent of pasteurellosis in wild and farmed marine fish worldwide. although serologically homogeneous, recent molecular advances have led to the discovery of distinct genetic clades, depending on geographical origin. further details of the strategies for host colonisation have arisen including information on the role of capsule, susceptibility to oxidative stress, confirmation of intracellular survival in host epithelial cells, and induced ... | 2005 | 15962471 |
| photobacterium damsela bacteremia in a child with sickle-cell disease. | photobacterium damsela was identified in a blood culture taken from a child with sickle-cell disease. this is the first report of this organism in humans in the caribbean. the microbiology of this organism and its identification are discussed. the clinical presentation in humans and the role of the immune status of the patient are reviewed. | 2005 | 15999016 |
| development of a simple and rapid fluorogenic procedure for identification of vibrionaceae family members. | we describe a simple colony overlay procedure for peptidases (copp) for the rapid fluorogenic detection and quantification of vibrionaceae from seawater, shellfish, sewage, and clinical samples. the assay detects phosphoglucose isomerase with a lysyl aminopeptidase activity that is produced by vibrionaceae family members. overnight cultures are overlaid for 10 min with membranes containing a synthetic substrate, and the membranes are examined for fluorescent foci under uv illumination. fluoresce ... | 2005 | 16000757 |
| photobacterium frigidiphilum sp. nov., a psychrophilic, lipolytic bacterium isolated from deep-sea sediments of edison seamount. | a gram-negative, motile, rod-shaped, psychrophilic and weakly halophilic bacterial strain, sl13(t), was isolated from deep-sea sediments (1450 m depth) of edison seamount in the western pacific ocean. optimal growth of sl13(t) requires the presence of 1.5% (w/v) nacl, a ph of 6.0 and a temperature of 14 degrees c. the whole-cell fatty acid profile of the isolate includes c16:1 and c16:0 as major fatty acids and contains c20:5omega3. this is consistent with corresponding data for photobacterium p ... | 2005 | 16014498 |
| synthesis, characterization, biocide and toxicological activities of di-n-butyl- and diphenyl-tin(iv)-salicyliden-beta-amino alcohol derivatives. | the one pot reaction of salicylaldehyde 1, beta-amino alcohols 2a-2c, and di-n-butyltin(iv) oxide 3a or diphenyltin(iv) oxide 3b produced five diorganotin(iv) compounds, 4a-4c, 5a, and 5c, in good yields. all compounds were characterized by ir, (1)h, (13)c, and (119)sn nmr spectroscopy, and elemental analysis; furthermore, compounds 4b, 4c, 5a, and 5c were characterized by x-ray diffraction analysis. after the structural characterization, all of the compounds were tested in vitro against bacillu ... | 2005 | 16022535 |
| nonbioluminescent strains of photobacterium phosphoreum produce the cell-to-cell communication signal n-(3-hydroxyoctanoyl)homoserine lactone. | bioluminescence is a common phenotype in marine bacteria, such as vibrio and photobacterium species, and can be quorum regulated by n-acylated homoserine lactones (ahls). we extracted a molecule that induced a bacterial ahl monitor (agrobacterium tumefaciens nt1 [pzlr4]) from packed cod fillets, which spoil due to growth of photobacterium phosphoreum. interestingly, ahls were produced by 13 nonbioluminescent strains of p. phosphoreum isolated from the product. of 177 strains of p. phosphoreum (i ... | 2005 | 15812045 |
| adhesion to sole, solea senegalensis kaup, mucus of microorganisms isolated from farmed fish, and their interaction with photobacterium damselae subsp. piscicida. | abstract most studies carried out to select microorganisms as candidate probiotics have focused on in vitro antagonism tests, such as the production of inhibitory compounds against pathogenic microorganisms. however, attachment to mucous surfaces could be another criterion to be considered when selecting potential probiotics for aquaculture. nineteen isolates obtained from farmed senegalese sole, solea senegalensis kaup, and gilthead sea bream, sparus aurata l., have been evaluated for their cap ... | 2005 | 15813865 |
| the effect of sea bream (sparus aurata) broodstock and larval vaccination on the susceptibility by photobacterium damsela subsp. piscicida and on the humoral immune parameters. | sea bream broodstock were immunised 1 or 2 months before spawning with a novel photobacteriosis vaccine. sixty-seven-day-old larvae (mean weight 22.3 mg) originating from immunised and non-immunised parents were experimentally infected with the photobacterium damsela subsp. piscicida (phdp). larvae from immunised fish showed delayed onset and lower mortality (66.67%) compared with larvae from control fish (80%). eighty-nine-day-old larvae (mean weight 162.2 mg) from both groups were bath vaccina ... | 2005 | 15863015 |
| heme uptake genes in human and fish isolates of photobacterium damselae: existence of huta pseudogenes. | the marine bacterium photobacterium damselae includes strains classified into two distinct subspecies, namely subsp. damselae and subsp. piscicida, which have been reported to cause disease in a variety of marine animals and in humans. p. damselae strains utilize heme compounds as sole iron sources. in the present study, ten potential heme uptake and utilization genes are described in p. damselae subsp. damselae and subsp. piscicida. one gene cluster includes the genes coding for putative protei ... | 2005 | 15918073 |
| characteristics of endogenous flavin fluorescence of photobacterium leiognathi luciferase and vibrio fischeri nad(p)h:fmn-oxidoreductase. | the bioluminescent bacterial enzyme system nad(p)h:fmn-oxidoreductase-luciferase has been used as a test system for ecological monitoring. one of the modes to quench bioluminescence is the interaction of xenobiotics with the enzymes, which inhibit their activity. the use of endogenous flavin fluorescence for investigation of the interactions of non-fluorescent compounds with the bacterial luciferase from photobacterium leiognathi and nad(p)h:fmn-oxidoreductase from vibrio fischeri has been propo ... | 2005 | 15924327 |
| significant histamine formation in tuna (thunnus albacares) at 2 degrees c--effect of vacuum- and modified atmosphere-packaging on psychrotolerant bacteria. | occurrence and importance of psychrotolerant histamine producing bacteria in chilled fresh tuna were demonstrated in the present study. the objective was to evaluate microbial formation of histamine and biogenic amines in chilled fresh tuna from the indian ocean and stored either vacuum-packed (vp) or modified atmosphere-packed (map). firstly, biogenic amines and the dominating microbiota were determined in vp tuna involved in an outbreak of histamine fish poisoning in denmark. secondly, the mic ... | 2005 | 15925710 |
| a simple hydrophobicity-based approach to predict the toxicity of unknown organic micropollutant mixtures in marine water. | in this study, we propose a simple hydrophobicity-based approach to predict the toxicity of mixtures of marine organic micropollutants. fifty mixtures were chosen, comprising individual chemicals randomly chosen from three halogenated benzenes, three phenols, two petroleum hydrocarbons, three polychlorinated biphenyls (pcbs), four organochlorine pesticides (ocps) and two herbicides, with the intention of simulating the mixtures of organic micropollutants that may exist in some marine waters. the ... | 2005 | 15935177 |
| [conditions that influence bacterial luminescence in the presence of blood serum]. | conditions that influence the luminescence of natural and recombinant luminescent bacteria in the presence of blood serum were studied. in general, blood serum quenched the luminescence of the marine photobacterium phosphoreum and the recombinant escherichia coli strains harboring the luminescent system genes of photobacterium leiognathi, but enhanced the luminescence of the soil bacterium photorhabdus luminescens zm1 and the recombinant e. coli strain harboring the lux operon of p. luminescens ... | 2005 | 15938395 |
| random mutagenesis of bacterial luciferase: critical role of glu175 in the control of luminescence decay. | bacterial luciferases (luxab) can be readily classed as slow or fast decay luciferases based on their rates of luminescence decay in a single turnover assay. luciferases from vibrio harveyi and xenorhabdus (photorhabdus) luminescens have slow decay rates, and those from the photobacterium genus, such as photobacterium fisheri, p. phosphoreum and p. leiognathi, have rapid decay rates. by substitution of a 67-amino-acid stretch of p. phosphoreum luxa in the central region of the luxa subunit, the ... | 2005 | 15352872 |
| influence of vaccination on the nitric oxide response of gilthead seabream following infection with photobacterium damselae subsp. piscicida. | the nitric oxide (no) response of vaccinated and non-vaccinated juvenile gilthead seabream was studied in vivo and the no response of isolated kidney macrophages of fish was studied in vitro. fish were vaccinated with formalin-killed photobacterium damselae subsp. piscicida (pdp) with or without freund's incomplete adjuvant (fia) and control fish received phosphate buffered saline (pbs). thirty days later, fish were injected with a sublethal dose of pdp and 3 fish/group were bled at time periods ... | 2005 | 15450966 |
| characteristic analysis of the ampc gene encoding beta-lactamase from photobacterium phosphoreum. | the ampc gene of photobacterium phosphoreum atcc 11040 was cloned and identified. nucleotide sequence of the regulatory region r&r and the ampc gene (genbank accession no. ay787792) from p. phosphoreum has been determined, and the encoded beta-lactamase is deduced. the beta-lactamase encoded by the ampc gene has a calculated m(r) 31,198 and comprises 285 amino acid residues (pi 7.35). there is a signal peptide of 20 amino acid residues mklrfiastlllsfsqlasa to lead the beta-lactamase secretion, a ... | 2005 | 15596133 |
| genome update: proteome comparisons. | 2005 | 15632419 | |
| photobacterium lipolyticum sp. nov., a bacterium with lipolytic activity isolated from the yellow sea in korea. | a gram-negative, motile, non-spore-forming, pleomorphic and lipolytic bacterial strain, m37t, was isolated from an intertidal sediment of the yellow sea in korea. this organism grew optimally at 25-28 degrees c and in the presence of 1-2 % nacl. it did not grow without nacl or in the presence of more than 6 % nacl. strain m37t was characterized chemotaxonomically by having q-8 as the predominant respiratory lipoquinone and c(16 : 1)omega7c and/or iso-c(15 : 0) 2-oh and c(16 : 0) as the major fat ... | 2005 | 15653897 |
| effectiveness of a divalent vaccine for sole, solea senegalensis (kaup), against vibrio harveyi and photobacterium damselae subsp. piscicida. | the protection of cultured sole, solea senegalensis, against vibrio harveyi and photobacterium damselae subsp. piscicida was evaluated following the use of a divalent vaccine prepared with formalized whole cells and extracellular products of virulent strains of both pathogenic microorganisms and administered by the immersion route. two prolonged immersions of 5-10 g fish in the divalent bacterin at a 1-month interval gave high levels of protection similar to those obtained when the respective mo ... | 2005 | 15660791 |
| influence of hydroxypropylcyclodextrins on the toxicity of mixtures. | we studied the influence of hydroxypropylcyclodextrins (hpcds) on the toxicity of some mixtures. using the photobacterium phosphoreum toxicity test, the joint toxicological effect for mixture i (containing p-nitrobenzaldehyde and 1-nitronaphthalene) and mixture ii (containing p-nitrobenzaldehyde and malononitrile) were determined in water and in aqueous solutions of hpcds. the results indicate that, although the toxicological joint effect for mixture i (simple addition) differs from that of mixt ... | 2005 | 15667850 |
| variation in 16s-23s rrna intergenic spacer regions in photobacterium damselae: a mosaic-like structure. | phenotypically, photobacterium damselae subsp. piscicida and p. damselae subsp. damselae are easily distinguished. however, their 16s rrna gene sequences are identical, and attempts to discriminate these two subspecies by molecular tools are hampered by their high level of dna-dna similarity. the 16s-23s rrna internal transcribed spacers (its) were sequenced in two strains of photobacterium damselae subsp. piscicida and two strains of p. damselae subsp. damselae to determine the level of molecul ... | 2005 | 15691912 |
| genomic and phylogenetic characterization of luminous bacteria symbiotic with the deep-sea fish chlorophthalmus albatrossis (aulopiformes: chlorophthalmidae). | bacteria forming light-organ symbiosis with deep-sea chlorophthalmid fishes (aulopiformes: chlorophthalmidae) are considered to belong to the species photobacterium phosphoreum. the identification of these bacteria as p. phosphoreum, however, was based exclusively on phenotypic traits, which may not discriminate between phenetically similar but evolutionarily distinct luminous bacteria. therefore, to test the species identification of chlorophthalmid symbionts, we carried out a genomotypic (repe ... | 2005 | 15691950 |
| molecular cloning and functional analysis of photobacterium damselae subsp. piscicida haem receptor gene. | a haem receptor gene from photobacterium damselae subsp. piscicida (formerly known as pasteurella piscicida) has been cloned, sequenced and analysed for its function. the gene, designated as pph, has an open reading frame consisting of 2154 bp, a predicted 718 amino acid residues and exists as a single copy. it is homologous with the haem receptors of vibrio anguillarum hupa, v. cholerae huta, v. mimicus mhua and v. vulnificus hupa at 32.7, 32.7, 45.6 and 30.9%, respectively, and is highly conse ... | 2005 | 15705153 |
| sensitivity of dark mutants of various strains of luminescent bacteria to reactive oxygen species. | recent studies indicated that bioluminescence of the marine bacterium vibrio harveyi may both stimulate dna repair and contribute to detoxification of deleterious oxygen derivatives. therefore, it was also proposed that these reactions can be considered biological roles of bacterial luminescence and might act as evolutionary drives in development of luminous systems. however, experimental evidence for the physiological role of luciferase in protection of cells against oxidative stress has been d ... | 2005 | 15717160 |
| side effects in sea bass (dicentrarchus labrax l.) due to intraperitoneal vaccination against vibriosis and pasteurellosis. | sea bass (dicentrarchus labrax l.) were injected intraperitoneally with monovalent (photobacterium damselae subsp. piscicida or vibrio anguillarum) and divalent (ph. damselae subsp. piscicida and v. anguillarum) vaccine formulations, with or without adjuvants (mineral oil, liposome or alginate), to evaluate the short and long-term pathological effects. eight animals from each group were sampled one, two, four and 11 months after intraperitoneal injection. the acute peritoneal response and the pr ... | 2005 | 15722227 |
| characterization of fe/mn-superoxide dismutase from diatom thallassiosira weissflogii: cloning, expression, and property. | a cdna clone of 1114 bp encoding a putative mn-superoxide dismutase (mn-sod) from diatom thallassiosira weissflogii was cloned by the pcr technique. nucleotide sequence analysis of this cdna clone revealed that it was translated into 201 amino acid residues. when the sequence was compared with mn-sods from vibrio mimicus and escherichia coli, as well as two fe-sods from e. coli and photobacterium leiognathi, this sod showed higher homology to mn-sod. the amino acid residues required to coordinat ... | 2005 | 15740026 |
| life at depth: photobacterium profundum genome sequence and expression analysis. | deep-sea life requires adaptation to high pressure, an extreme yet common condition given that oceans cover 70% of earth's surface and have an average depth of 3800 meters. survival at such depths requires specific adaptation but, compared with other extreme conditions, high pressure has received little attention. recently, photobacterium profundum strain ss9 has been adopted as a model for piezophily. here we report its genome sequence (6.4 megabase pairs) and transcriptome analysis. the result ... | 2005 | 15746425 |
| toxicity of agricultural dust extract using microtox. | 2005 | 15768505 |