Publications

functional and structural analysis of a highly-expressed yersinia pestis small rna following infection of cultured macrophages.	non-coding small rnas (srnas) are found in practically all bacterial genomes and play important roles in regulating gene expression to impact bacterial metabolism, growth, and virulence. we performed transcriptomics analysis to identify srnas that are differentially expressed in yersinia pestis that invaded the human macrophage cell line thp-1, compared to pathogens that remained extracellular in the presence of host. using ultra high-throughput sequencing, we identified 37 novel and 143 previou ...	2016	28030576
plague vaccine development: current research and future trends.	plague is one of the world's most lethal human diseases caused by yersinia pestis, a gram-negative bacterium. despite overwhelming studies for many years worldwide, there is no safe and effective vaccine against this fatal disease. inhalation of y. pestis bacilli causes pneumonic plague, a fast growing and deadly dangerous disease. f1/lcrv-based vaccines failed to provide adequate protection in african green monkey model in spite of providing protection in mice and cynomolgus macaques. there is ...	2016	28018363
structure-activity relationships of the mepicides: n-acyl and o-linked analogs of fr900098 as inhibitors of dxr from mycobacterium tuberculosis and yersinia pestis.	despite continued research efforts, the threat of drug resistance from a variety of bacteria continues to plague clinical communities. discovery and validation of novel biochemical targets will facilitate development of new drugs to combat these organisms. the methylerythritol phosphate (mep) pathway to make isoprene units is a biosynthetic pathway essential to many bacteria. we and others have explored inhibitors of the mep pathway as novel antibacterial agents. mycobacterium tuberculosis, the ...	2016	27676224
paleogenetics and past infections: the two faces of the coin of human immune evolution.	with the advent of next-generation sequencing, paleogenetics has considerably expanded over the past few years and notably encompassed the characterization of the genomes of archaic humans who lived more than 30,000 years ago. these paleogenetics investigations have revealed that admixture between modern and archaic humans occurred, with neanderthals having contributed to 1.5% to 2.1% of modern eurasian genomes, and denisovans to 3% to 6% of modern melanesian genomes and to approximately 0.2% of ...	2016	27337483
selectivity of pyridone- and diphenyl ether-based inhibitors for the yersinia pestis fabv enoyl-acp reductase.	the enoyl-acp reductase (enr) catalyzes the last reaction in the elongation cycle of the bacterial type ii fatty acid biosynthesis (fas-ii) pathway. while the fabi enr is a well-validated drug target in organisms such as mycobacterium tuberculosis and staphylococcus aureus, alternate enr isoforms have been discovered in other pathogens, including the fabv enzyme that is the sole enr in yersinia pestis (ypfabv). previously, we showed that the prototypical enr inhibitor triclosan was a poor inhibi ...	2016	27136302
beyond helper phage: using "helper cells" to select peptide affinity ligands.	peptides are important affinity ligands for microscopy, biosensing, and targeted delivery. however, because they can have low affinity for their targets, their selection from large naïve libraries can be challenging. when selecting peptidic ligands from display libraries, it is important to: 1) ensure efficient display; 2) maximize the ability to select high affinity ligands; and 3) minimize the effect of the display context on binding. the "helper cell" packaging system has been described as a ...	2016	27626637
bacterial pathogens activate plasminogen to breach tissue barriers and escape from innate immunity.	both coagulation and fibrinolysis are tightly connected with the innate immune system. infection and inflammation cause profound alterations in the otherwise well-controlled balance between coagulation and fibrinolysis. many pathogenic bacteria directly exploit the host's hemostatic system to increase their virulence. here, we review the capacity of bacteria to activate plasminogen. the resulting proteolytic activity allows them to breach tissue barriers and evade innate immune defense, thus pro ...	2016	26485450
host-parasite associations in small mammal communities in semiarid savanna ecosystems of east africa.	despite the established importance of rodents as reservoirs of vector-borne zoonoses in east africa, there is relatively limited information regarding the infestation parameters and host associations of ectoparasites that vector many such pathogens among small mammals in this region. between 2009 and 2013, small mammals were live-trapped in the semiarid savanna of kenya. a subset of these individual hosts, including 20 distinct host taxa, was examined for ectoparasites, which were identified to ...	2016	27113102
replacing arginine 33 for alanine in the hemophore hasa from pseudomonas aeruginosa causes closure of the h32 loop in the apo-protein.	previous characterization of hemophores from serratia marcescens (hasas), pseudomonas aeruginosa (hasap), and yersinia pestis (hasayp) showed that hemin binds between two loops, where it is axially coordinated by h32 and y75. the y75 loop is structurally conserved in all three hemophores and harbors conserved ligand y75. the other loop contains h32 in hasas and hasap, but a noncoordinating q32 in hasayp. the h32 loop in apo-hasas and apo-hasap is in an open conformation, which places h32 about 3 ...	2016	27074415
a replication-defective human type 5 adenovirus-based trivalent vaccine confers complete protection against plague in mice and nonhuman primates.	currently, no plague vaccine exists in the united states for human use. the capsular antigen (caf1 or f1) and two type 3 secretion system (t3ss) components, the low-calcium-response v antigen (lcrv) and the needle protein yscf, represent protective antigens of yersinia pestis we used a replication-defective human type 5 adenovirus (ad5) vector and constructed recombinant monovalent and trivalent vaccines (rad5-lcrv and rad5-yfv) that expressed either the codon-optimized lcrv or the fusion gene d ...	2016	27170642
occurrence and analysis of irp2 virulence gene in isolates of klebsiella pneumoniae and enterobacter spp. from microbiota and hospital and community-acquired infections.	eighty-five isolates of klebsiella pneumoniae and enterobacter spp., originating from hospital- and community-acquired infections and from oropharyngeal and faecal microbiota from patients in recife-pe, brazil, were analyzed regarding the presence of irp2 gene. this is a yersinia typical gene involved in the synthesis of siderophore yersiniabactin. dna sequencing confirmed the identity of irp2 gene in five k. pneumoniae, five enterobacter aerogenes and one enterobacter amnigenus isolates. to our ...	2016	27133266
structural and functional characterization of the lps transporter lptde from gram-negative pathogens.	incorporation of lipopolysaccharide (lps) into the outer membrane of gram-negative bacteria is essential for viability, and is accomplished by a two-protein complex called lptde. we solved crystal structures of the core lptde complexes from yersinia pestis, klebsiella pneumoniae, pseudomonas aeruginosa, and a full-length structure of the k. pneumoniae lptde complex. our structures adopt the same plug and 26-strand β-barrel architecture found recently for the shigella flexneri and salmonella typh ...	2016	27161977
intranasal delivery of a protein subunit vaccine using a tobacco mosaic virus platform protects against pneumonic plague.	yersinia pestis, one of history's deadliest pathogens, has killed millions over the course of human history. it has attributes that make it an ideal choice to produce mass casualties and is a prime candidate for use as a biological weapon. when aerosolized, y. pestis causes pneumonic plague, a pneumonia that is 100% lethal if not promptly treated with effective antibiotics. currently, there is no fda approved plague vaccine. the current lead vaccine candidate, a parenterally administered protein ...	2016	27745954
β-hydroxyacyl-acyl carrier protein dehydratase (fabz) from francisella tularensis and yersinia pestis: structure determination, enzymatic characterization, and cross-inhibition studies.	the bacterial system for fatty acid biosynthesis (fas) contains several enzymes whose sequence and structure are highly conserved across a vast array of pathogens. this, coupled with their low homology and difference in organization compared to the equivalent system in humans, makes the fas pathway an excellent target for antimicrobial drug development. to this end, we have cloned, expressed, and purified the β-hydroxyacyl-acyl carrier protein dehydratase (fabz) from both francisella tularensis ...	2016	26818694
novel engineered cationic antimicrobial peptides display broad-spectrum activity against francisella tularensis, yersinia pestis and burkholderia pseudomallei.	broad-spectrum antimicrobials are needed to effectively treat patients infected in the event of a pandemic or intentional release of a pathogen prior to confirmation of the pathogen's identity. engineered cationic antimicrobial peptides (ecaps) display activity against a number of bacterial pathogens including multi-drug-resistant strains. two lead ecaps, wlbu2 and wr12, were compared with human cathelicidin (ll-37) against three highly pathogenic bacteria: francisella tularensis, yersinia pesti ...	2016	26673248
a self-quenching-resistant carbon nanodot powder with multicolored solid-state fluorescence for ultra-fast staining of various representative bacterial species within one minute.	in this study, we prepared self-quenching-resistant solid-state fluorescent carbon nanodots (sfcds) without any other solid matrices. the sfcds were prepared using a one-step microwave synthesis method through precise control of the heating power and time. the resulting sfcd powder showed excitation-dependent emission behavior with a maximum fluorescence quantum yield of 40%. the multicolored sfcds were successfully used as fluorescent agents for rapid staining of 14 representative bacterial spe ...	2016	27874136
fast, sensitive point of care electrochemical molecular system for point mutation and select agent detection.	point of care molecular diagnostics benefits from a portable battery-operated device capable of performing a fast turnaround using reliable inexpensive cartridges. we describe a prototype device for performing a molecular diagnostics test for clinical and biodefense samples in 16 minutes using a prototype capable of an 8 minute pcr reaction, followed by hybridization and detection on an electrochemical microarray based on the i-stat® system. we used human buccal swabs for hemochromatosis testing ...	2016	27280174
an internal standard approach for homogeneous tr-fret immunoassays facilitates the detection of bacteria, biomarkers, and toxins in complex matrices.	the recent development of a homogeneous time-resolved förster resonance energy transfer (tr-fret) immunoassay enables one-step, rapid (minutes), and direct detection compared to the multistep, time-consuming (hours), heterogeneous elisa-type immunoassays. the use of the time-resolved effect of a donor lanthanide complex with a delay time of microseconds and large stokes shift enables the separation of positive signals from the background autofluorescence of the sample. however, this study shows ...	2016	27236318
rapid antimicrobial susceptibility testing of bacillus anthracis, yersinia pestis, and burkholderia pseudomallei by use of laser light scattering technology.	rapid methods to determine antimicrobial susceptibility would assist in the timely distribution of effective treatment or postexposure prophylaxis in the aftermath of the release of bacterial biothreat agents such as bacillus anthracis, yersinia pestis, or burkholderia pseudomallei conventional susceptibility tests require 16 to 48 h of incubation, depending on the bacterial species. we evaluated a method that is based on laser light scattering technology that measures cell density in real time. ...	2016	26984973
a personal view of how paleomicrobiology aids our understanding of the role of lice in plague pandemics.	we have been involved in the field of paleomicrobiology since 1998, when we used dental pulp to identify yersinia pestis as the causative agent of the great plague of marseille (1720). we recently designed a specific technique, "suicide pcr," that can prevent contamination. a controversy arose between two teams, with one claiming that dna must be altered to amplify it and the other group claiming that demographic data did not support the role of y. pestis in the black death (i.e., the great plag ...	2016	27726806
molecular survey of bartonella species and yersinia pestis in rodent fleas (siphonaptera) from chihuahua, mexico.	rodent fleas from northwestern chihuahua, mexico, were analyzed for the presence of bartonella and yersinia pestis. in total, 760 fleas belonging to 10 species were tested with multiplex polymerase chain reaction analysis targeting the glta (338-bp) and pla genes (478-bp) of bartonella and y. pestis, respectively. although none was positive for y. pestis, 307 fleas were infected with bartonella spp., resulting in an overall prevalence of 40.4%. a logistic regression analysis indicated that the p ...	2016	26576933
an update on the hazards and risks of forensic anthropology, part ii: field and laboratory considerations.	this paper focuses on potential hazards and risks to forensic anthropologists while working in the field and laboratory in north america. much has changed since galloway and snodgrass published their seminal article addressing these issues. the increased number of forensic practitioners combined with new information about potential hazards calls for an updated review of these pathogens and chemicals. discussion of pathogen hazards (brucella, borrelia burgdorferi, yersinia pestis, clostridium tet ...	2016	26389711
evolution and structural dynamics of bacterial glycan binding adhesins.	infectious disease processes like bacterial adherence or the activity of secreted toxins frequently gain host and tissue specificity by glycan binding interactions with the host glycome. recent functional and structural studies highlight the high niche specialization of bacterial lectins, but also reveal a remarkable plasticity in their glycan binding sites and mechanisms, to adapt to host glycome dynamics or changing environmental conditions at the site of infection. in this review we put empha ...	2016	28043017
a recombinant trivalent fusion protein f1-lcrv-hsp70(ii) augments humoral and cellular immune responses and imparts full protection against yersinia pestis.	plague is one of the most dangerous infections in humans caused by yersinia pestis, a gram-negative bacterium. despite of an overwhelming research success, no ideal vaccine against plague is available yet. it is well established that f1/lcrv based vaccine requires a strong cellular immune response for complete protection against plague. in our earlier study, we demonstrated that hsp70(ii) of mycobacterium tuberculosis modulates the humoral and cellular immunity of f1/lcrv vaccine candidates indi ...	2016	27458447
pterin-sulfa conjugates as dihydropteroate synthase inhibitors and antibacterial agents.	the sulfonamide class of antibiotics has been in continuous use for over 70years. they are thought to act by directly inhibiting dihydropteroate synthase (dhps), and also acting as prodrugs that sequester pterin pools by forming dead end pterin-sulfonamide conjugates. in this study, eight pterin-sulfonamide conjugates were synthesized using a novel synthetic strategy and their biochemical and microbiological properties were investigated. the conjugates were shown to competitively inhibit dhps, a ...	2016	27423480
[pilot-scale purification of rf1-v fusion protein of yersinia pestis and characterization of its immunogenicity].	recombinant fl-v (rfl-v) fusion protein is the main ingredient of the current candidate vaccine against yersinia pestis infection, which has been under investigation in clinical trial in usa. we investigated the soluble expression conditions of rf1-v in escherichia coli bl21 (de3) that we constructed before. after scale-up and optimization of fermentation processes, we got the optimized fermentation process parameters: the culture was induced at the middle exponential phase with 50 µmol/l of ipt ...	2016	27363202
highly effective soluble and bacteriophage t4 nanoparticle plague vaccines against yersinia pestis.	plague caused by yersinia pestis is an ancient disease, responsible for millions of deaths in human history. unfortunately, there is no fda-approved vaccine available. recombinant subunit vaccines based on two major antigens, caf 1 (f1) and lcrv (v), have been under investigation and showed promise. however, there are two main problems associated with these vaccines. first, the yersinia capsular protein f1 has high propensity to aggregate, particularly when expressed in heterologous systems such ...	2016	27076150
quaternary structure of fur proteins, a new subfamily of tetrameric proteins.	the ferric uptake regulator (fur) belongs to the family of the dna-binding metal-responsive transcriptional regulators. fur is a global regulator found in all proteobacteria. it controls the transcription of a wide variety of genes involved in iron metabolism but also in oxidative stress or virulence factor synthesis. when bound to ferrous iron, fur can bind to specific dna sequences, called fur boxes. this binding triggers the repression or the activation of gene expression, depending on the re ...	2016	26886069
the yersiniabactin-associated atp binding cassette proteins ybtp and ybtq enhance escherichia coli fitness during high-titer cystitis.	the yersinia high-pathogenicity island (hpi) is common to multiple virulence strategies used by escherichia coli strains associated with urinary tract infection (uti). among the genes in this island are ybtp and ybtq, encoding distinctive atp binding cassette (abc) proteins associated with iron(iii)-yersiniabactin import in yersinia pestis in this study, we compared the impact of ybtpq on a model e. coli cystitis strain during in vitro culture and experimental murine infections. a ybtpq-null mut ...	2016	26883590
bacterial effector nanoparticles as breast cancer therapeutics.	bacterial pathogens trigger cell death by a variety of mechanisms, including injection of effector proteins. effector proteins have great potential as anticancer agents because they efficiently subvert a variety of eukaryotic signaling pathways involved in cancer development, drug resistance, and metastasis. in breast cancer, mapk and nfκb pathways are known to be dysregulated. yopj, an effector from yersinia pestis, downregulates mapk and nfκb pathways to induce cell death in specific cell type ...	2016	26800341
recombinant murine toxin from yersinia pestis shows high toxicity and β-adrenergic blocking activity in mice.	yersinia pestis murine toxin (ymt) encoded on pmt1 is a 61-kda protein, a member of the phospholipase d superfamily, which is found in all the domains of life. it is considered to be an intracellular protein required for the survival of y. pestis in the midgut of the flea, but the exact role of ymt in the pathogenesis of y. pestis has not been clarified. purified ymt is highly toxic to mice and rats, but the exact mechanism of the animals' death is unclear. here, we prepared a recombinant ymt in ...	2016	26774329
navigable rivers facilitated the spread and recurrence of plague in pre-industrial europe.	infectious diseases have become a rising challenge to mankind in a globalizing world. yet, little is known about the inland transmission of infectious diseases in history. in this study, we based on the spatio-temporal information of 5559 plague (yersinia pestis) outbreaks in europe and its neighboring regions in ad1347-1760 to statistically examine the connection between navigable rivers and plague outbreak. our results showed that 95.5% of plague happened within 10 km proximity of navigable ri ...	2016	27721393
inhibition of pseudomonas aeruginosa exsa dna-binding activity by n-hydroxybenzimidazoles.	the pseudomonas aeruginosa type iii secretion system (t3ss) is a primary virulence determinant and a potential target for antivirulence drugs. one candidate target is exsa, a member of the arac family of dna-binding proteins required for expression of the t3ss. a previous study identified small molecules based on an n-hydroxybenzimidazole scaffold that inhibit the dna-binding activity of several arac proteins, including exsa. in this study, we further characterized a panel of n-hydroxybenzimidaz ...	2016	26574012
engineered nanoconstructs for the multiplexed and sensitive detection of high-risk pathogens.	many countries categorize the causative agents of severe infectious diseases as high-risk pathogens. given their extreme infectivity and potential to be used as biological weapons, a rapid and sensitive method for detection of high-risk pathogens (e.g., bacillus anthracis, francisella tularensis, yersinia pestis, and vaccinia virus) is highly desirable. here, we report the construction of a novel detection platform comprising two units: (1) magnetic beads separately conjugated with multiple capt ...	2016	26462853
crp is an activator of yersinia pestis biofilm formation that operates via a mechanism involving gmha and waaae-coad.	gmha encodes a phosphoheptose isomerase that catalyzes the biosynthesis of heptose, a conserved component of lipopolysaccharide (lps). gmha plays an important role in yersinia pestis biofilm blockage in the flea gut. waaa, waae, and coad constitute a three-gene operon waaae-coad in y. pestis. waaa encodes a transferase that is responsible for binding lipid-a to the core oligosaccharide of lps. waaa is a key determinant in y. pestis biofilm formation, and the waaa expression is positively regulat ...	2016	27014218
nqrm (duf539) protein is required for maturation of bacterial na+-translocating nadh:quinone oxidoreductase.	na(+)-translocating nadh:quinone oxidoreductase (na(+)-nqr) catalyzes electron transfer from nadh to ubiquinone in the bacterial respiratory chain, coupled with na(+) translocation across the membrane. na(+)-nqr maturation involves covalent attachment of flavin mononucleotide (fmn) residues, catalyzed by flavin transferase encoded by the nqr-associated apbe gene. analysis of complete bacterial genomes has revealed another putative gene (duf539, here renamed nqrm) that usually follows the apbe ge ...	2016	26644436
detection of a yersinia pestis gene homologue in rodent samples.	a homologue to a widely used genetic marker, pla, for yersinia pestis has been identified in tissue samples of two species of rat (rattus rattus and rattus norvegicus) and of mice (mus musculus and apodemus sylvaticus) using a microarray based platform to screen for zoonotic pathogens of interest. samples were from urban locations in the uk (liverpool) and canada (vancouver). the results indicate the presence of an unknown bacterium that shares a homologue for the pla gene of yersinia pestis, so ...	2016	27602258
genomic insights into a new citrobacter koseri strain revealed gene exchanges with the virulence-associated yersinia pestis ppcp1 plasmid.	the history of infectious diseases raised the plague as one of the most devastating for human beings. far too often considered an ancient disease, the frequent resurgence of the plague has led to consider it as a reemerging disease in madagascar, algeria, libya, and congo. the genetic factors associated with the pathogenicity of yersinia pestis, the causative agent of the plague, involve the acquisition of the ppcp1 plasmid that promotes host invasion through the expression of the virulence fact ...	2016	27014253
reciprocal regulation of yersinia pestis biofilm formation and virulence by rovm and rova.	rova is known to enhance yersinia pestis virulence by directly upregulating the psa loci. this work presents a complex gene regulatory paradigm involving the reciprocal regulatory action of rovm and rova on the expression of biofilm and virulence genes as well as on their own genes. rovm and rova enhance and inhibit y. pestis biofilm production, respectively, whereas rovm represses virulence in mice. rovm directly stimulates the transcription of hmst, hmscde and rovm, while indirectly enhancing ...	2016	26984293
new role for fda-approved drugs in combating antibiotic-resistant bacteria.	antibiotic resistance in medically relevant bacterial pathogens, coupled with a paucity of novel antimicrobial discoveries, represents a pressing global crisis. traditional drug discovery is an inefficient and costly process; however, systematic screening of food and drug administration (fda)-approved therapeutics for other indications in humans offers a rapid alternative approach. in this study, we screened a library of 780 fda-approved drugs to identify molecules that rendered raw 264.7 murine ...	2016	27067323
biocidal and sporicidal efficacy of pathoster(®) 0.35% and pathoster(®) 0.50% against bacterial agents in potential bioterrorism use.	the use of products that can neutralize or significantly reduce the microbial load and that are not harmful to human health and the environment represents a milestone in the fight against the spread of infectious diseases. peracetic acid, besides being an excellent sterilizing and sporicidal agent, is harmless to humans and the environment when it is used in a common dosage. however, the high costs and loss of efficacy of the product very quickly after its reconstitution limit its use. we evalua ...	2016	27482880
how old are bacterial pathogens?	only few molecular studies have addressed the age of bacterial pathogens that infected humans before the beginnings of medical bacteriology, but these have provided dramatic insights. the global genetic diversity of helicobacter pylori, which infects human stomachs, parallels that of its human host. the time to the most recent common ancestor (tmrca) of these bacteria approximates that of anatomically modern humans, i.e. at least 100 000 years, after calibrating the evolutionary divergence withi ...	2016	27534956
targeted next-generation sequencing for the detection of ciprofloxacin resistance markers using molecular inversion probes.	antibiotic resistance (ar) is an epidemic of increasing magnitude requiring rapid identification and profiling for appropriate and timely therapeutic measures and containment strategies. in this context, ciprofloxacin is part of the first-line of countermeasures against numerous high consequence bacteria. significant resistance can occur via single nucleotide polymorphisms (snp) and deletions within ciprofloxacin targeted genes. ideally, use of ciprofloxacin would be prefaced with ar determinati ...	2016	27174456
genetic variation at the mhc drb1 locus is similar across gunnison's prairie dog (cynomys gunnisoni) colonies regardless of plague history.	yersinia pestis was introduced to north america around 1900 and leads to nearly 100% mortality in prairie dog (cynomys spp.) colonies during epizootic events, which suggests this pathogen may exert a strong selective force. we characterized genetic diversity at an mhc class ii locus (drb1) in gunnison's prairie dog (c. gunnisoni) and quantified population genetic structure at the drb1 versus 12 microsatellite loci in three large arizona colonies. two colonies, seligman (se) and espee ranch (es), ...	2016	27066243
development of liposomal ciprofloxacin to treat lung infections.	except for management of pseudomonas aeruginosa (pa) in cystic fibrosis, there are no approved inhaled antibiotic treatments for any other diseases or for infections from other pathogenic microorganisms such as tuberculosis, non-tuberculous mycobacteria, fungal infections or potential inhaled biowarfare agents including francisella tularensis, yersinia pestis and coxiella burnetii (which cause pneumonic tularemia, plague and q fever, respectively). delivery of an antibiotic formulation via the i ...	2016	26938551
[infection prevention and surgical management of deep insidious infection in total endoprosthesis].		2016	5084870
cost containment--and quality assurance, too. 2.		2016	5082628
the epidermal component of pityriasis lichenoides.		2016	5057378
national health care.		2016	5059462
[comparative estimation of the corticoid and immunosuppressive therapy in nephrotic syndrome].		2016	5023105
mesenteric fibrosis simulating the angiographic appearance of ileal carcinoid tumor.		2016	5015843
[serum concentrations of phenoxymethylpenicillin in a controlled study on volunteers].		2016	5001919
cephaloridine in the treatment and prophylaxis of infection in the newborn.		2016	4964001
[histological study of autografts of dental roots in the eyelid of macacus rhesus. ii].		2016	4991838
cerebral electrical activity following soman administration.		2016	4973155
placentation in the garter snake, thamnophis sirtalis.		2016	4912310
[problems of drug research].		2016	4874723
individual differences at birth. implications for child-care practices.		2016	4852409
vegetables?		2016	4847369
[current status of experience and concepts concerning the therapy of progressive muscular dystrophies].		2016	4889952
psychology and neuropsychology in migraine.		2016	4949988
[x-ray diagnosis of early functional gallbladder disorders using a computer].		2016	4931629
chemical conversion of the psychotomimetic amine 1-(2,5-dimethoxy-4-methylphenyl)-2-aminopropane to 5-hydroxy-2,6-dimethylindole.		2016	4836406
urinary tract fibrinolysis.		2016	4831731
clomipramine and amitriptyline in depressed outpatients. a controlled study.		2016	4812659
comparison of oral prednisolone and intramuscular methylprednisolone in classical rheumatoid arthritis in out-patients.		2016	4931625
a resuscitation trolley designed for a communication problem.		2016	4821283
preparing lower-class patients for group psychotherapy: development and evaluation of a role-induction film.		2016	4803270
[neurologic, electroencephalographic and brain scintigraphic findings in supratentorial space-requiring processes].		2016	4811132
neonatal cold injury in the tropics.		2016	4782010
[umberto carpi de resmini].		2016	4918468
[changes in mitotic activity of cells of the epidermis in pseudoepitheliomatous hyperplasia and spinocellular cancer of the skin].		2016	4767949
ivory-tower humanist.		2016	4763433
proceedings: levels of blood pressure in children: a family study.		2016	4759734
neurologic manifestations of rheumatic fever.		2016	4759729
significance of the curriculum and prerequisites for the certification of corrective therapists.		2016	4752267
experimental studies on the value of archery in paraplegia.		2016	4750964
unexpected vitamin k deficiency in hospitalized patients.	twenty-seven patients developed vitamin k deficiency unexpectedly in the postoperative period or during hospitalization for a variety of medical conditions. twenty-two of the patients were on antibiiotics. nineteen were on no oral intake during part of their hospital stay, and the remainder had a poor food intake. fifty percent developed vitamin k deficiency within seven days. it is suggested that the rapid onset of vitamin k deficiency in these patients may have been due to a combination of the ...	2016	4750303
shock and septic complications.		2016	4746120
whitefringed beetles: dichlorvos applied as a postharvest treatment to protect wheat.		2016	4734780
[pruritus therapy in the light of spa methods].		2016	4728797
[synovectomy of the hand].		2016	4712009
granulomatous hepatitis and prolonged fever of unknown origin.		2016	4729250
the twin ion technique for detection of metabolites by gas chromatography-mass spectrometry: intermediates in estrogen biosynthesis.		2016	4711114
[synoptic demonstration of antiepidemic measures].		2015	4641580
evidence for biosynthetical equivalence of the epimeric isoleucine residues in angolide.		2015	4337566
transfer of metabolites across the nuclear membrane. a microfluorometric study.		2015	4327736
the effect of paroven (hr) on the clearance of sodium-24 from the subcutaneous tissues of the foot in patients with varicose veins.		2015	4745138
[acute adaline poisoning].		2015	4384384
[massive doses of thiamine in neurosurgical resuscitation].		2015	4690563
the preoperative interview and psychological evaluation of the orthognathic surgery patient.		2015	4526365
prognosis of cutaneous malignant melanoma: a clinicopathological study.		2015	4422540
[administration of corticosteroids to patients with hormone-producing tumors of the adrenal cortex].		2015	4457702
the vascular supply of eosinophilic and mixed pituitary adenomas in acromegalic patients.		2015	4363893
non-filarial elephantiasis of the lower legs in ethiopia. a simple method for rapid survey by school enquiry.		2015	4693995
non-filarial elephantiasis of the lower legs in ethiopia. a simple method for rapid survey by school enquiry.		2015	4693995
maxillary arch correction in cleft lip and palate cases.		2015	4913662
maxillary arch correction in cleft lip and palate cases.		2015	4913662
[effects of 2 antimetabolites on the development of aedes aegypti larvae].		2015	4662010
ethanol sleep times and hepatic alcohol and aldehyde dehydrogenase activities in mice.		2015	4658590