Publications
Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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independent emergence of yersinia ruckeri biotype 2 in the united states and europe. | biotype 2 (bt2) variants of the bacterium yersinia ruckeri are an increasing disease problem in u.s. and european aquaculture and have been characterized as serovar 1 isolates that lack both peritrichous flagella and secreted phospholipase activity. the emergence of this biotype has been associated with an increased frequency of enteric redmouth disease (erm) outbreaks in previously vaccinated salmonid fish. in this study, four independent specific natural mutations that cause the loss of both m ... | 2011 | 21441334 |
diversity of somatic coliphages in coastal regions with different levels of anthropogenic activity in sao paulo state, brazil. | bacteriophages are the most abundant and genetically diverse viruses on earth, with their complex ecology in both quantitative and qualitative terms. somatic coliphages (sc) have been reported to be good indicators of fecal pollution in seawater. this study focused on determining the concentration of sc and their diversity by electron microscopy in seawater, plankton and bivalve samples collected at three coastal regions são paulo, brazil. the sc counts varied from <1 to 3.4×10(3) pfu/100ml in s ... | 2011 | 21531842 |
a yersinia effector with enhanced inhibitory activity on the nf-κb pathway activates the nlrp3/asc/caspase-1 inflammasome in macrophages. | a type iii secretion system (t3ss) in pathogenic yersinia species functions to translocate yop effectors, which modulate cytokine production and regulate cell death in macrophages. distinct pathways of t3ss-dependent cell death and caspase-1 activation occur in yersinia-infected macrophages. one pathway of cell death and caspase-1 activation in macrophages requires the effector yopj. yopj is an acetyltransferase that inactivates mapk kinases and ikkβ to cause tlr4-dependent apoptosis in naïve ma ... | 2011 | 21533069 |
utilization of nitrophenylphosphates and oxime-based ligation for the development of nanomolar affinity inhibitors of the yersinia pestis outer protein h (yoph) phosphatase. | our current study reports the first k(m) optimization of a library of nitrophenylphosphate-containing substrates for generating an inhibitor lead against the yersinia pestis outer protein phosphatase (yoph). a high activity substrate identified by this method (k(m) = 80 µm) was converted from a substrate into an inhibitor by replacement of its phosphate group with difluoromethylphosphonic acid and by attachment of an aminooxy handle for further structural optimization by oxime ligation. a cocrys ... | 2011 | 21443195 |
high catalytic efficiency and resistance to denaturing in bacterial rho gtpase-activating proteins. | abstract several major bacterial pathogens use the type iii secretion system (ttss) to deliver virulence factors into host cells. bacterial rho gtpase activating proteins (rhogaps) comprise a remarkable family of type iii secreted toxins that modulate cytoskeletal dynamics and manipulate cellular signaling pathways. we show that the rhogap activity of salmonella sptp and pseudomonas exos toxins is resistant to variations in the concentration of nacl or mgcl(2), unlike the known salt dependant na ... | 2011 | 21534865 |
[analysis of insect toxin complex gene variability of yersinia pestis and yersinia pseudotuberculosis strains]. | the nucleotide sequences of the tc's insect toxin complex genes have been analyzed in 18 natural strains of the main and non-main subspecies of yersinia pestis isolated in different natural foci in the russian federation, as well as neighboring and more remote countries, as compared to the data on y. pestis and y. pseudotuberculosis strains stored in the ncbi genbank database. the nucleotide sequences of these genes in plague agent strains have been found to be highly conserved, in contrast to t ... | 2011 | 21446179 |
shotgun sequencing of yersinia enterocolitica strain w22703 (biotype 2, serotype o:9): genomic evidence for oscillation between invertebrates and mammals. | yersinia enterocolitica strains responsible for mild gastroenteritis in humans are very diverse with respect to their metabolic and virulence properties. strain w22703 (biotype 2, serotype o:9) was recently identified to possess nematocidal and insecticidal activity. to better understand the relationship between pathogenicity towards insects and humans, we compared the w22703 genome with that of the highly pathogenic strain 8081 (biotype1b; serotype o:8), the only y. enterocolitica strain sequen ... | 2011 | 21453472 |
trimeric autotransporter adhesin-dependent adherence of bartonella henselae, bartonella quintana and yersinia enterocolitica to matrix components and endothelial cells under static and dynamic flow conditions. | trimeric autotransporter adhesins (taas) are important virulence factors of gram-negative bacteria responsible for adherence to extracellular matrix (ecm) and host cells. here, we analyzed three different taas [bartonella adhesin a (bada) of bartonella henselae, variably expressed outer membrane proteins (vomps) of bartonella quintana, yersinia adhesin a (yada) of yersinia enterocolitica] for mediating bacterial adherence to ecm and endothelial cells. using static (cell culture vials) and dynami ... | 2011 | 21536788 |
a comprehensive study on the role of the yersinia pestis virulence markers in an animal model of pneumonic plague. | we determined the role of yersinia pestis virulence markers in an animal model of pneumonic plague. eleven strains of y. pestis were characterized using pcr assays to detect the presence of known virulence genes both encoded by the three plasmids as well as chromosomal markers. the virulence of all y. pestis strains was compared in a mouse model for pneumonic plague. the presence of all known virulence genes correlated completely with virulence in the balb/c mouse model. strains which lacked hms ... | 2011 | 21468758 |
safety and efficacy of anti-tumor necrosis factor α therapy in ten patients with recent-onset refractory reactive arthritis. | 2011 | 21538314 | |
automated lipid a structure assignment from hierarchical tandem mass spectrometry data. | infusion-based electrospray ionization (esi) coupled to multiple-stage tandem mass spectrometry (ms(n)) is a standard methodology for investigating lipid a structural diversity (shaffer et al. j. am. soc. mass. spectrom. 18(6), 1080-1092, 2007). annotation of these ms(n) spectra, however, has remained a manual, expert-driven process. in order to keep up with the data acquisition rates of modern instruments, we devised a computational method to annotate lipid a ms(n) spectra rapidly and automatic ... | 2011 | 21472520 |
cra negatively regulates acid survival in yersinia pseudotuberculosis. | survival in acidic environments is important for successful infection of gastrointestinal pathogens. many bacteria have evolved elaborate mechanisms by inducing or repressing gene expression, which subsequently provide ph homeostasis and enable acid survival. in this study, we employed comparative proteomic analysis to identify the acid-responsive proteins of a food-borne enteric bacterium, yersinia pseudotuberculosis. the expression level of eight proteins involved in carbohydrate metabolism wa ... | 2011 | 21276044 |
hepatic yersiniosis caused by yersinia enterocolitica 4:o3 in an adult dog. | a 2-year-old pekinese dog was diagnosed with hepatic yersiniosis. grossly, white-to-yellow nodules consisting of degenerated inflammatory cells, cell debris, and bacterial clumps were scattered throughout the liver. histopathologically, suppurative and necrotizing hepatitis was apparent. yersinia enterocolitica biotype 4, serotype o3 (4:o3) was identified and confirmed in the liver immunohistochemically, using a monoclonal antibody. the virulence genes ysta and ail were detected, but the isolate ... | 2011 | 21398468 |
the main virulence determinant of yersinia entomophaga mh96 is a broad-host-range toxin complex active against insects. | through transposon mutagenesis and dna sequence analysis, the main disease determinant of the entomopathogenic bacterium yersinia entomophaga mh96 was localized to an ~32-kb pathogenicity island (pai) designated pai(ye96). residing within pai(ye96) are seven open reading frames that encode an insecticidal toxin complex (tc), comprising not only the readily recognized toxin complex a (tca), tcb, and tcc components but also two chitinase proteins that form a composite tc molecule. the central tc g ... | 2011 | 21278295 |
different enteropathogenic yersinia strains found in wild boars and domestic pigs. | yersinia enterocolitica and yersinia pseudotuberculosis strains isolated from wild boars and fattening pigs were characterized and compared with each other. in wild boars, ail-positive y. enterocolitica strains belonged to bioserotypes 4/o:3 (36%, 5/14), 2/o:9 (29%, 4/14), and 2/o:5,27 (21%, 3/14). additionally, two ail-positive strains were untypable. among fattening pigs, the bioserotype 4/o:3 was dominating (91%, 71/78), and bioserotypes 2/o:5,27 (8%, 6/78) and 2/o:9 (1%, 1/78) were rare. inv ... | 2011 | 21288132 |
dietary administration of beta-mercapto-ethanol treated saccharomyces cerevisiae enhanced the growth, innate immune response and disease resistance of the rainbow trout, oncorhynchus mykiss. | the effects of dietary whole cell yeast (saccharomyces cerevisiae), n-3 hufa-enriched yeast and treated yeast cells with beta-mercapto-ethanol (2me) on immunity, growth performance and disease resistance to yersinia ruckeri were investigated in oncorhynchus mykiss. during 30 days, juvenile rainbow trout were fed diets supplemented with different forms of yeast at 5 × 10(7) cfu g(-1) or a control diet. after the feeding trial, remaining fish of each treatment were challenged by pathogenic yersini ... | 2011 | 21296672 |
plague in guinea pigs and its prevention by subunit vaccines. | human pneumonic plague is a devastating and transmissible disease for which a food and drug administration-approved vaccine is not available. suitable animal models may be adopted as a surrogate for human plague to fulfill regulatory requirements for vaccine efficacy testing. to develop an alternative to pneumonic plague in nonhuman primates, we explored guinea pigs as a model system. on intranasal instillation of a fully virulent strain, yersinia pestis co92, guinea pigs developed lethal lung i ... | 2011 | 21406168 |
complete genome sequence of yersinia enterocolitica subsp. palearctica serogroup o:3. | we report here the first finished and annotated genome sequence of a representative of the most epidemiologically successful yersinia group, y. enterocolitica subsp. palearctica strain y11, serotype o:3, biotype 4. this strain is a certified type strain of the german dsmz collection (dsm no. 13030; yersinia enterocolitica subsp. palearctica) that was isolated from the stool of a human patient (h. neubauer, s. aleksic, a. hensel, e. j. finke, and h. meyer. int. j. med. microbiol. 290:61-64, 2000) ... | 2011 | 21296963 |
comparison of etest method with reference broth microdilution method for antimicrobial susceptibility testing of yersinia pestis. | the utility of etest for antimicrobial susceptibility testing of yersinia pestis was evaluated in comparison with broth microdilution and disk diffusion for eight agents. four laboratories tested 26 diverse strains and found etest to be reliable for testing antimicrobial agents used to treat y. pestis, except for chloramphenicol and trimethoprim-sulfamethoxazole. disk diffusion testing is not recommended. | 2011 | 21411569 |
novel non-motile phenotypes of yersinia ruckeri suggest expansion of the current clonal complex theory. | the biochemical and cell surface characteristics of 63 non-motile isolates of yersinia ruckeri from various sources were compared using the api 20e rapid identification system and conventional phenotypic methods. eight individual phenotypic groups from a variety of fish species were observed from the data set. non-motile isolates were not exclusively observed from serogroup o1; membership of biotype 2 was recorded for representatives from serogroups o2-o7. variations in phenotypes highlights tha ... | 2011 | 21320135 |
yersinia enterocolitica bacteremia in a chronic, mildly iron-overloaded dialysis patient (february). | objective: to report an unusual case of yersinia enterocolitica in a chronic, mildly iron-overloaded dialysis patient lacking other typical risk factors for bacteremia, who was treated successfully with a third-generation cephalosporin as monotherapy for a short duration of treatment. case summary: a 76-year-old dialysis-dependent man developed y. enterocolitica bacteremia after 8 days of hospitalization. one month prior to presentation, his ferritin level was mildly elevated at 571.5 ng/ml, whi ... | 2011 | 21266629 |
physical, chemical and microbiological quality of ice used to cool drinks and foods in greece and its public health implications. | ice used for direct human consumption or to preserve foods and cool down drinks can be contaminated with pathogenic microorganisms and may potentially become a vehicle for consumer's infection. to evaluate physical, chemical and microbiological quality of commercial ice and ice used for fish and seafood, 100 ice samples collected at 10 different retail points in the region of epirus were studied. the following microbiological parameters were determined: total coliforms, fecal coliforms, salmonel ... | 2011 | 21802520 |
yersiniabactin iron uptake: mechanisms and role in yersinia pestis pathogenesis. | yersiniabactin (ybt) is a siderophore-dependent iron uptake system encoded on a pathogenicity island that is widespread among pathogenic bacteria including the yersiniae. while biosynthesis of the siderophore has been elucidated, the secretion mechanism and a few components of the uptake/utilization pathway are unidentified. ybt genes are transcriptionally repressed by fur but activated by ybta, likely in combination with the siderophore itself. the ybt system is essential for the ability of yer ... | 2011 | 21609780 |
[purification of recombinant h-ns protein of yersinia pestis and characterization of its dna-binding activity]. | the regulator protein h-ns of yersinia pestis was expressed using the escherichia coli bl21lambdade3 protein expression system, and its dna-binding activity was characterized. | 2011 | 21800623 |
an individual-based simulation of pneumonic plague transmission following an outbreak and the significance of intervention compliance. | the existence of primary pneumonic plague outbreaks raises concerns over the use of the causative bacteria as an aerosol-based bioweapon. we employed an individual-based model, parameterised using published personal contact information, to assess the severity of a deliberate release in a discrete community, under the influence of two proposed intervention strategies. we observed that the severity of the resulting epidemic is determined by the degree of personal compliance with said strategies, i ... | 2011 | 21624780 |
transcriptional regulation of ompf2, an ompf paralogue, in yersinia pestis. | a regulatory circuit composed of three porins (ompf, ompc, and ompx) and two transcriptional regulators (ompr and crp) has previously been characterized in yersinia pestis . in this follow-up study, ompf2, an ompf paralogue, was integrated into this regulatory circuit. only basal expression was detected for ompf2 in the wild-type strain under different osmotic conditions. the ompf2 transcription was dramatically enhanced with increasing medium osmolarity in the ompr null mutant background. the c ... | 2011 | 21627465 |
[interaction of plague microbe strains varying in plasmid composition with the fleas xenopsylla cheopis (roths. 1903)]. | the interaction of two yersinia pestis strains varying in plasmid composition with the fleas xenopsylla cheopis was studied. the reference virulent strain i-2638 having four plasmids (pcad, ppst, pfra, and ptp 33) and its selected avirulent strain i-3480 that had lost the plasmids pcad and ppst formed a proventricular block in the fleas with equal frequency. there were no differences in the block-forming capacity of these strains among the infected females; however, the stock strain was more act ... | 2011 | 21797060 |
in vitro efficacy of antibiotics commonly used to treat human plague against intracellular yersinia pestis. | yersinia pestis initiates infection as a facultative intracellular parasite in host macrophages; however, little is known about the efficacy of antibiotics commonly used to treat human plague against intracellular y. pestis. intracellular minimal bactericidal concentrations were determined using a high throughput broth microdilution assay in which human thp-1 macrophage-like cells were infected with y. pestis strain kim6-2053.1+ and exposed to two-fold serial dilutions of antibiotics for 24 h in ... | 2011 | 21628541 |
structure and function of martx toxins and other large repetitive rtx proteins. | the repeats-in-toxins (rtx) family of proteins classically consists of cytolysins and hemolysins. over the past decade, genome sequencing revealed the existence of very large members of this family. these are all repetitive proteins ranging in size from 200 to 900 kda that function as toxins or adhesins. many are exported by type i secretion. one major new subfamily is the large repetitive rtx adhesins and biofilm-associated proteins. these are characterized by 80- to 300- amino-acid repeats ord ... | 2011 | 21639783 |
yersinia pestis tir-domain protein forms dimers that interact with the human adaptor protein myd88. | recent research has highlighted the presence of toll/interleukin 1 receptor (tir)-domain proteins (tdps) in a range of bacteria, suggested to form interactions with the human adaptor protein myd88 and inhibit intracellular signaling from toll-like receptors (tlrs). a tdp has been identified in yersinia pestis (yptdp), a highly pathogenic bacterium responsible for plague. expression of a number of yptir constructs of differing lengths (yptir1, s130-a285; yptir2, i137-i273; yptir3, i137-246; yptir ... | 2011 | 21640812 |
[clinical features, diagnosis and treatment of 5 cases of primary pneumonic plague in tibet in 2010]. | to explore the clinical manifestations, the feature of chest x-ray, the clinical outcome, and the clinical treatments of severe pneumonic plague. | 2011 | 21781509 |
[antimicrobial activity of bacteriocin s760 produced by enterococcus faecium strain lwp760]. | antimicrobial activity of bacteriocin s760 (enterocin) produced by enterococcusfaecium strain lwp760 was studied. bacteriocin s760 is a cationic, hydrophobic, and heat stable peptide with the molecular weight of 5.5 kda and pl of 9.8. enterocin s760 is shown to inhibit in vitro the growth both of sensitive and resistant to antibacterials gramnegative and grampositive bacteria of 25 species. mics of the bacteriocin s760 vary between 0.05-1.6 mg/l for escherichia coli 0157:h117, salmonella typhimu ... | 2011 | 21780664 |
yersinia pestis yope contains a dominant cd8 t cell epitope that confers protection in a mouse model of pneumonic plague. | septic bacterial pneumonias are a major cause of death worldwide. several of the highest priority bioterror concerns, including anthrax, tularemia, and plague, are caused by bacteria that acutely infect the lung. bacterial resistance to multiple antibiotics is increasingly common. although vaccines may be our best defense against antibiotic-resistant bacteria, there has been little progress in the development of safe and effective vaccines for pulmonary bacterial pathogens. the gram-negative bac ... | 2011 | 21653834 |
multi-functional characteristics of the pseudomonas aeruginosa type iii needle-tip protein, pcrv; comparison to orthologs in other gram-negative bacteria. | pseudomonas aeruginosa possesses a type iii secretion system (t3ss) to intoxicate host cells and evade innate immunity. this virulence-related machinery consists of a molecular syringe and needle assembled on the bacterial surface, which allows delivery of t3 effector proteins into infected cells. to accomplish a one-step effector translocation, a tip protein is required at the top end of the t3 needle structure. strains lacking expression of the functional tip protein fail to intoxicate host ce ... | 2011 | 21772833 |
identification of the lps core of yersinia pestis and yersinia pseudotuberculosis as the receptor for bacteriophage {varphi}a1122. | ?a1122 is t7-related bacteriophage infecting most isolates of yersinia pestis, the etiologic agent of plague, and used by cdc in the identification of y. pestis. ?a1122 infects y. pestis grown both at 20°c and at 37°c. wild type y. pseudotuberculosis strains are also infected, but only when grown at 37°c. since y. pestis expresses rough lipopolysaccharide (lps) missing the o-polysaccharide (o-ps) and expression of y. pseudotuberculosis o-ps is largely suppressed at temperatures above 30°c it has ... | 2011 | 21764935 |
trends of the major porin gene (ompf) evolution: insight from the genus yersinia. | ompf is one of the major general porins of enterobacteriaceae that belongs to the first line of bacterial defense and interactions with the biotic as well as abiotic environments. porins are surface exposed and their structures strongly reflect the history of multiple interactions with the environmental challenges. unfortunately, little is known on diversity of porin genes of enterobacteriaceae and the genus yersinia especially. we analyzed the sequences of the ompf gene from 73 yersinia strains ... | 2011 | 21655186 |
low resolution structure of a bacterial slc26 transporter reveals dimeric stoichiometry and mobile intracellular domains. | the slc26/sulp (solute carrier/sulfate transporter) proteins are a superfamily of anion transporters conserved from bacteria to man, of which four have been identified in human diseases. proteins within the slc26/sulp family exhibit a wide variety of functions, transporting anions from halides to carboxylic acids. the proteins comprise a transmembrane domain containing between 10-12 transmembrane helices followed a by c-terminal cytoplasmic sulfate transporter and anti-sigma factor antagonist (s ... | 2011 | 21659513 |
enhancement of immune response to an antigen delivered by vaccinia virus by displaying the antigen on the surface of intracellular mature virion. | vaccinia virus (vacv) is the vaccine for smallpox and a widely used vaccine vector for infectious diseases and cancers. the majority of the antibodies elicited by live vacv vaccination respond to virion structural proteins, including many integral membrane proteins on the intracellular mature virion (mv). here, we showed that antibody response to an exogenous antigen delivered by vacv was greatly enhanced by incorporating the antigen as an integral membrane protein of mv. we constructed recombin ... | 2011 | 21664218 |
yersinia pestis in small rodents, mongolia. | to the editor: plague is known to be endemic in several areas of mongolia, but transmission to humans seems to play only a minor role because the number of recognized cases is relatively low (figure) (1). the first human cases in mongolia were reported to the world health organization in 1980, and <20 human cases have occurred each year since then (2). however, human plague was first reported in 1897 (3), such infections have been documented since the 1940s, and yersinia pestis can be found in m ... | 2011 | 21762605 |
identification and characterization of a new true lipase isolated through metagenomic approach. | abstract: background: metagenomics, the application of molecular genomics to consortia of non-cultivated microbes, has the potential to have a substantial impact on the search for novel industrial enzymes such as esterases (carboxyl ester hydrolases, ec 3.1.1.1) and lipases (triacylglycerol lipases, ec 3.1.1.3). in the current work, a novel lipase gene was identified from a fosmid metagenomic library constructed with the "prokaryotic-enriched" dna from a fat-contaminated soil collected from a wa ... | 2011 | 21762508 |
progress on plague vaccine development. | yersinia pestis (yp), the gram-negative plague bacterium, has shaped human history unlike any other pathogen known to mankind. yp (transmitted by the bite of an infected flea) diverged only recently from the related enteric pathogen yersinia pseudotuberculosis but causes radically different diseases. three forms of plague exist in humans: bubonic (swollen lymph nodes or bubos), septicemic (spread of yp through the lymphatics or bloodstream from the bubos to other organs), and contagious, pneumon ... | 2011 | 21670978 |
antioxidant, electrochemical, thermal, antimicrobial and alkane oxidation properties of tridentate schiff base ligands and their metal complexes. | in this study, two schiff base ligands (hl(1) and hl(2)) and their cu(ii), co(ii), ni(ii), pd(ii) and ru(iii) metal complexes were synthesized and characterized by the analytical and spectroscopic methods. alkane oxidation activities of the metal complexes were studied on cyclohexane as substrate. the ligands and their metal complexes were evaluated for their antimicrobial activity against corynebacterium xerosis, bacillus brevis, bacillus megaterium, bacillus cereus, mycobacterium smegmatis, st ... | 2011 | 21752697 |
oxime-based click chemistry in the development of 3-isoxazolecarboxylic acid containing inhibitors of yersinia pestis protein tyrosine phosphatase, yoph. | the pathogenicity of yersinia pestis relies on several effector proteins including yoph, a protein tyrosine phosphatase (ptp). we previously screened a library of analogues based on the ubiquitous ptp substrate para-nitrophenylphosphate (pnpp) and found that incorporation of a 3-phenyl substituent to give 6-nitro-[1,1'-biphenyl]-3-yldihydrogen phosphate (1) enhanced affinity. herein we report the conversion of 1 from a substrate into an inhibitor by replacing the hydrolysable phosphoryl group wi ... | 2011 | 21671403 |
varying dependency of periplasmic peptidyl-prolyl cis/trans isomerases in promoting yersinia pseudotuberculosis stress tolerance and pathogenicity. | periplasmic peptidyl-prolyl cis/trans isomerases (ppiases) catalyze the cis/trans isomerization of peptidyl-prolyl bonds, which is a rate-limiting step during protein folding. we demonstrate that the sura, ppia, ppid fkpa and fklb alleles each encode a periplasmic ppiase in the bacterial pathogen yersinia pseudotuberculosis. of these, four were purified to homogeneity. purified sura, fkpa and fklb, but not ppid, displayed detectable ppiase activity in vitro. significantly, only y. pseudotubercul ... | 2011 | 21726196 |
structural characterization and membrane localization of exsb from the type iii secretion system (t3ss) of pseudomonas aeruginosa. | pseudomonas aeruginosa is an opportunistic human pathogen that employs a finely tuned type iii secretion system (t3ss) to inject toxins directly into the cytoplasm of target cells. exsb is a 15.6-kda protein encoded in a t3ss transcription regulation operon that displays high sequence similarity to yscw, a lipoprotein from yersinia spp. whose genetic neighborhood also involves a transcriptional regulator, and has been shown to play a role in the stabilization of the outer membrane ring of the t3 ... | 2011 | 21839744 |
quantitative proteomic analysis reveals formation of an escl-escq-escn type iii complex in enteropathogenic e. coli. | we characterized two type iii secretion (t3s) system proteins orf5 and sepq in enteropathogenic escherichia coli and showed that they are essential for t3s, associated with the bacterial membrane, and interact with escn. our findings suggest that orf5 and sepq are the yscl and yscq homologs from yersinia, respectively. | 2011 | 21804003 |
the effect of quercus castaneifolia extract on pathogenic enteric bacteria. | the family of enterobacteriaceae is a major group of gram negative bacteria, some of these microorganisms are pathogen and could cause disease mainly gastroenteritis. recently, due to drug resistant nature of these bacteria specially in developing countries treatment of the patient considered as important investigate. quercus castaneifolia is a native plant of yasuj province in iran, which the people who living in this area consume the fruit of this plant for treatment of enteric disease. hence, ... | 2011 | 21827865 |
t cells play an essential role in anti-f1 mediated rapid protection against bubonic plague. | plague, which is initiated by yersinia pestis infection, is a fatal disease that progresses rapidly and leads to high mortality rates if not treated. antibiotics are an effective plague therapy, but antibiotic-resistant y. pestis strains have been reported and therefore alternative countermeasures are needed. in the present study, we assessed the potential of an f1 plus lcrv-based vaccine to provide protection shortly pre- or post-exposure to a lethal y. pestis infection. mice vaccinated up to o ... | 2011 | 21803090 |
tnfrp55 modulates il-6 and nitric oxide responses following yersinia lipopolysaccharide stimulation in peritoneal macrophages. | while cytokines are major regulators of macrophage activation following host-pathogen interactions, they also act to limit inflammation to avoid tissue damage. in previous studies we reported the development of progressive yersinia enterocolitica-induced reactive arthritis (rea) in mice lacking the tumor necrosis factor receptor p55 (tnfrp55). in this work, we analyzed the response of tnfrp55(-/-) macrophages to y. enterocolitica antigens. we found higher concentration of nitric oxide (no) in tn ... | 2011 | 21802165 |
a specific inorganic triphosphatase from nitrosomonas europaea: structure and catalytic mechanism. | the cyth superfamily of proteins is named after its two founding members, the cyab adenylyl cyclase from aeromonas hydrophila and the human 25-kda thiamine triphosphatase. because these proteins often form a closed +¦-barrel, they are also referred to as "triphosphate tunnel metalloenzymes" (ttm). functionally, they are characterized by their ability to bind triphosphorylated substrates and divalent metal ions. these proteins exist in most organisms and catalyze different reactions, depending on ... | 2011 | 21840996 |
comparison of broth microdilution and disk diffusion test for antimicrobial resistance testing in yersinia enterocolitica 4/o:3 strains. | the aim of this study was to compare the results obtained from two methods for the determination of antimicrobial resistance in 110 yersinia enterocolitica 4/o:3 strains. ten antimicrobial agents were tested using broth microdilution and disk diffusion. similar results were determined for six antimicrobials. very major errors (false-susceptible by disk diffusion test) were detected for ampicillin (at a rate of 1.8%). major errors (false-resistant by disk diffusion test) were found for streptomyc ... | 2011 | 21568753 |
cellular and molecular action of the mitogenic protein-deamidating toxin from pasteurella multocida. | the mitogenic toxin from pasteurella multocida (pmt) is a member of the dermonecrotic toxin family, which includes toxins from bordetella, escherichia coli and yersinia. members of the dermonecrotic toxin family modulate g-protein targets in host cells through selective deamidation and/or transglutamination of a critical active site gln residue in the g-protein target, which results in the activation of intrinsic gtpase activity. structural and biochemical data point to the uniqueness of pmt amo ... | 2011 | 21569202 |
growth of salmonella enteritidis and salmonella typhimurium in the presence of quorum sensing signalling compounds produced by spoilage and pathogenic bacteria. | the effect of acylated homoserine lactones (ahls) and autoinducer-2 (ai-2) signalling compounds present in the cell-free culture supernatants (cfs), of pseudomonas aeruginosa, yersinia enterocolitica-like gte 112, serratia proteamaculans 00612, y. enterocolitica city650 and y. enterocolitica city844, on the growth of two salmonella enteritidis and two s. typhimurium strains was assessed though monitoring of changes in conductance of the medium. detection times (t(det)), area and slope of conduct ... | 2011 | 21569946 |
recombinant (f1+v) vaccine protects cynomolgus macaques against pneumonic plague. | cynomolgus macaques, immunised at the 80μg dose level with an rf1+rv vaccine (two doses, three weeks apart), were fully protected against pneumonic plague following inhalational exposure to a clinical isolate of yersinia pestis (strain co92) at week 8 of the schedule. at this time, all the immunised animals had developed specific igg titres to rf1 and rv with geometric mean titres of 96.83±20.93μg/ml and 78.59±12.07μg/ml, respectively, for the 40μg dose group; by comparison, the 80μg dose group ... | 2011 | 21570437 |
the yopj superfamily in plant-associated bacteria. | bacterial pathogens employ the type iii secretion system to secrete and translocate effector proteins into their hosts. the primary function of these effector proteins is believed to be the suppression of host defence responses or innate immunity. however, some effector proteins may be recognized by the host and consequently trigger a targeted immune response. the yopj/hopz/avrrxv family of bacterial effector proteins is a widely distributed and evolutionarily diverse family, found in both anima ... | 2011 | 21726386 |
investigation of bacterial and viral agents and immune status in behcet's disease patients from iran. | aim:ôçé beh+ºet's disease (bd) is an autoimmune disorder associated with hla-b51 positivity. serologic/genomic findings have suggested microbes as possible causative agents and the geographical distribution suggests environmental influences. methods:ôçé we performed comparative analyses of 40 patients with bd or related symptoms not fulfilling bd criteria. patients originating from different regions of iran were tested by molecular/serological methods for human herpes viruses and parvovirus b19, ... | 2011 | 21816027 |
growth model of a plasmid-bearing virulent strain of yersinia pseudotuberculosis in raw ground beef. | the growth kinetics of virulence plasmid-bearing yersinia pseudotuberculosis (ypst) in sterile ground beef were studied at temperatures ranging from 0 to 30°c. in irradiated sterile ground beef, ypst replicated from 0 to 30°c, with corresponding growth rates (gr) ranging from 0.023 to 0.622 log cfu/h at 0-25°c, and the gr was 0.236 log cfu/h at 30°c. the maximum population densities (mpd) ranged from 8.7 to 11.0 log cfu/g. the growth and mpd of ypst were reduced significantly at 30°c. models for ... | 2011 | 19912603 |
rapid field detection assays for bacillus anthracis, brucella spp., francisella tularensis and yersinia pestis. | rapid detection is essential for timely initiation of medical post-exposure prophylactic measures in the event of intentional release of biological threat agents. we compared real-time pcr assay performance between the applied biosystems 7300/7500 and the razor instruments for specific detection of the causative agents of anthrax, brucellosis, tularemia and plague. furthermore, an assay detecting bacillus thuringiensis, a bacillus anthracis surrogate, was developed for field-training purposes. a ... | 2011 | 20132255 |
development of a loop-mediated isothermal amplification assay for the detection of mycobacterium bovis. | bovine tuberculosis caused by mycobacterium bovis is an important zoonosis. in this study, a simple, rapid method for detecting this organism was developed based on loop-mediated isothermal amplification of the mpt83 gene. the technique will be of value in the clinical and field-based diagnosis of m. bovis and can differentiate it from other bacteria such as corynebacterium diphtheriae, streptococcus pneumoniae, β-haemolytic streptococci, pseudomonas aeruginosa, yersinia pseudotuberculosis, stap ... | 2011 | 20138787 |
variation in the prevalence of enteropathogenic yersinia in slaughter pigs from belgium, italy, and spain. | tonsils of 829 fattening pigs originating from belgium (n = 201), italy (n = 428), and spain (n = 200) were collected between 2005 and 2007 to study the prevalence of enteropathogenic yersinia in slaughter pigs. isolation of yersinia enterocolitica and yersinia pseudotuberculosis was done by selective enrichment and by cold enrichment for 7 and 14 days. pathogenic y. enterocolitica and y. pseudotuberculosis isolates were identified by polymerase chain reaction targeting the chromosomal genes ail ... | 2011 | 20187744 |
genetic characterisation and structural analysis of the o-specific polysaccharide of yersinia pseudotuberculosis serotype o:1c. | many, but not all, of the current 21 serotypes of yersinia pseudotuberculosis have been investigated with regard to the chemical structures of their o-specific polysaccharide (ops) and the genetic basis of their biosynthesis. completion of the genetics and structures of the remaining serotypes will enhance our understanding of the emerging relationship between genetics and structures within this species. here, we present a structural and genetic analysis of the y. pseudotuberculosis serotype o:1 ... | 2011 | 20418261 |
molecular cloning of cecropin b responsive endonucleases in yersinia ruckeri. | we have previously demonstrated that yersinia ruckeri resists cecropin b in an inducible manner. in this study, we sought to identify the molecular changes responsible for the inducible cecropin b resistance of y. ruckeri. differences in gene expression associated with the inducible resistance were investigated. cultures of y. ruckeri were exposed to a sublethal concentration of cecropin b and resultant changes in the messenger rna population of the bacteria were assayed using the differential d ... | 2011 | 20352273 |
emended description of yersinia massiliensis. | the bacterial genus yersinia belongs to the family enterobacteriaceae and comprises 15 species. species of the genus yersinia are usually identified by their phenotypic characteristics. thus, it is essential to establish a complete phenotypic classification for all species of the genus yersinia. the species yersinia massiliensis was proposed in 2008, based on 16s rrna, gyrb, hsp60, rpob and soda gene sequences and some distinguishing phenotypic characteristics. in this study, four yersinia strai ... | 2011 | 20525812 |
yersinia entomophaga sp. nov., isolated from the new zealand grass grub costelytra zealandica. | a gram-negative, rod-shaped, non-spore-forming bacterium (mh96(t)) was isolated from diseased larvae of the new zealand grass grub, costelytra zealandica (coleoptera: scarabaeidae). on the basis of 16s rrna gene sequence similarity, strain mh96(t) is a member of the genus yersinia, which is a member of the class gammaproteobacteria. the most similar 16s rrna gene sequence to that of mh96(t) is that of the type strain of yersinia mollaretii (98.5 % similarity) followed by those of the type strain ... | 2011 | 20495033 |
aflp genome scan in the black rat (rattus rattus) from madagascar: detecting genetic markers undergoing plague-mediated selection. | the black rat (rattus rattus) is the main reservoir of plague (yersinia pestis infection) in madagascar's rural zones. black rats are highly resistant to plague within the plague focus (central highland), whereas they are susceptible where the disease is absent (low altitude zone). to better understand plague wildlife circulation and host evolution in response to a highly virulent pathogen, we attempted to determine genetic markers associated with plague resistance in this species. to this purpo ... | 2011 | 20444082 |
primary pneumonic plague in the african green monkey as a model for treatment efficacy evaluation. | primary pneumonic plague is rare among humans, but treatment efficacy may be tested in appropriate animal models under the fda 'animal rule'. | 2011 | 20722770 |
comparative susceptibility of atlantic salmon and rainbow trout to yersinia ruckeri: relationship to o antigen serotype and resistance to serum killing. | a study was undertaken to compare the virulence and serum killing resistance properties of atlantic salmon and rainbow trout yersinia ruckeri isolates. five isolates, covering heat-stable o-antigen o1, o2 and o5 serotypes, were tested for virulence towards fry and juveniles of both species by experimental bath challenge. the sensitivity of 15 diverse isolates to non-immune salmon and rainbow trout serum was also examined. all five isolates caused significant mortality in salmon fry. serotype o1 ... | 2011 | 20691549 |
transgenic carrot tap roots expressing an immunogenic f1-v fusion protein from yersinia pestis are immunogenic in mice. | expression of the protective f1 and v antigens of yersinia pestis, as a fusion protein, in carrot was pursued in an effort to develop an alternative vaccine production system against the serious plague disease. transgenic carrot plants carrying the f1-v encoding gene were developed via agrobacterium-mediated transformation. presence, integration, and expression of the f1-v encoding gene were confirmed by polymerase chain reaction (pcr), dna gel blot analysis, and reverse-transcriptase (rt)-pcr a ... | 2011 | 20655621 |
mus spretus seg/pas mice resist virulent yersinia pestis, under multigenic control. | laboratory mice are well known to be highly susceptible to virulent strains of yersinia pestis in experimental models of bubonic plague. we have found that mus spretus-derived seg/pas (seg) mice are exceptionally resistant to virulent co92 and 6/69 wild type strains. upon subcutaneous injection of 10(2) colony-forming units (cfu), 90% of females and 68% of males survived, compared with only an 8% survival rate for both male and female c57bl/6 mice. furthermore, half of the seg mice survived a ch ... | 2011 | 20861861 |
population genetic structure of the prairie dog flea and plague vector, oropsylla hirsuta. | oropsylla hirsuta is the primary flea of the black-tailed prairie dog and is a vector of the plague bacterium, yersinia pestis. we examined the population genetic structure of o. hirsuta fleas collected from 11 prairie dog colonies, 7 of which had experienced a plague-associated die-off in 1994. in a sample of 332 o. hirsuta collected from 226 host individuals, we detected 24 unique haplotype sequences in a 480 nucleotide segment of the cytochrome oxidase ii gene. we found significant overall po ... | 2011 | 20696095 |
outbreak of gastroenteritis caused by yersinia pestis in afghanistan. | plague, which is most often caused by the bite of yersinia pestis-infected fleas, is a rapidly progressing, serious disease that can be fatal without prompt antibiotic treatment. in late december 2007, an outbreak of acute gastroenteritis occurred in nimroz province of southern afghanistan. of the 83 probable cases of illness, 17 died (case fatality 20·5%). being a case was associated with consumption or handling of camel meat (adjusted odds ratio 4·4, 95% confidence interval 2·2-8·8, p<0·001). ... | 2011 | 20663260 |
ompr controls yersinia enterocolitica motility by positive regulation of flhdc expression. | flagella and invasin play important roles during the early stages of infection by the enteric pathogen yersinia enterocolitica. our previous study demonstrated that ompr negatively regulates invasin gene expression at the transcriptional level. the present study focused on the role of ompr in the regulation of flagella expression. motility assays and microscopic observations revealed that an ompr mutant strain exhibits a non-motile phenotype due to the lack of flagella. an analysis of flhdc::lac ... | 2011 | 20830609 |
sampling port for real-time analysis of bioaerosol in whole body exposure system for animal aerosol model development. | multiple factors influence the viability of aerosolized bacteria. the delivery of aerosols is affected by chamber conditions (humidity, temperature, and pressure) and bioaerosol characteristics (particle number, particle size distribution, and viable aerosol concentration). measurement of viable aerosol concentration and particle size is essential to optimize viability and lung delivery. the madison chamber is widely used to expose small animals to infectious aerosols. | 2011 | 20849964 |
salmonella typhimurium st213 is associated with two types of inca/c plasmids carrying multiple resistance determinants. | salmonella typhimurium st213 was first detected in the mexican typhimurium population in 2001. it is associated with a multi-drug resistance phenotype and a plasmid-borne blacmy-2 gene conferring resistance to extended-spectrum cephalosporins. the objective of the current study was to examine the association between the st213 genotype and blacmy-2 plasmids. | 2011 | 21223599 |
factors related to the prevalence of pathogenic yersinia enterocolitica on pig farms. | summarya survey of 788 pigs from 120 farms was conducted to determine the within-farm prevalence of pathogenic yersinia enterocolitica and a questionnaire of management conditions was mailed to the farms afterwards. a univariate statistical analysis with carriage and shedding as outcomes was conducted with random-effects logistic regression with farm as a clustering factor. variables with a p value <0â·15 were included into the respective multivariate random-effects logistic regression model. th ... | 2011 | 21226982 |
tapping the potential of intact cell mass spectrometry with a combined data analytical approach applied to yersinia spp.: detection, differentiation and identification of y. pestis. | in the everyday routine of an analytic lab, one is often confronted with the challenge to identify an unknown microbial sample lacking prior information to set the search limits. in the present work, we propose a workflow, which uses the spectral diversity of a commercial database (saramis) to narrow down the search field at a certain taxonomic level, followed by a refined classification by supervised modelling. as supervised learning algorithm, we have chosen a shrinkage discriminant analysis a ... | 2011 | 21239132 |
the ail gene is present in some yersinia enterocolitica biotype 1a strains. | one chromosomal virulence marker of yersinia is the gene ail, which encodes ail, an outer membrane protein that promotes attachment and invasion. a high correlation has been found between the ail gene and the virulence of yersinia. here, we report two yersinia enterocolitica biotype 1a strains that are usually nonpathogenic and carry the ail gene. the ail gene sequences of biotype 1a strains displayed similarity to the bioserotype 1b/o:8 strain 8081. the finding suggests that ail-based detection ... | 2011 | 21254915 |
yersinia enterocolitica outer protein t (yopt). | pathogenic yersinia strains evade the innate immune responses of the host by producing effector proteins (yersinia outer proteins (yops)), which are directly injected into mammalian cells by a type iii secretion system (ttss). one of these effector proteins (yopt) disrupts the actin cytoskeleton of the host cell. yopt is a cysteine protease which cleaves rho proteins directly upstream of the post-translationally modified cysteine. thereby, it releases the gtpases from the membrane leading to the ... | 2011 | 21255864 |
[the prevalence of yersinia infection in adult patients with acute right lower quadrant pain]. | clinical manifestations of intestinal yersiniosis include enterocolitis, mesenteric adenitis, and terminal ileitis presenting with fever, right lower quadrant pain, and leukocytosis. according to a previous korean study in 1997, yersinia was revealed in two among 15 adult patients with mesenteric adenitis (13%). however, recent reports on the prevalence of yersinia infection in adult patients are few. the aim of this study was to investigate the prevalence of yersinia infection in adult patients ... | 2011 | 21258196 |
chemical composition and antimicrobial activity of the essential oils of anthospermum emirnense and anthospermum perrieri (rubiaceae). | the essential oils of anthospermum emirnense baker and anthospermum perrieri homolle ex puff, obtained by hydrodistillation in 0.03 and 0.02% yield, respectively, were analyzed by gc/ms. in both cases, the major constituents consisted of sesquiterpene hydrocarbons and oxygenated sesquiterpenes. the two species showed an important qualitative similarity, with 40 compounds common to a. emirnense and a. perrieri, including β-elemene, trans-β-caryophyllene, caryophyllene oxide, and τ-cadinol, which ... | 2011 | 21259425 |
characterization of the rcsc sensor kinase from erwinia amylovora and other enterobacteria. | rcsc is a hybrid sensor kinase which contains a sensor domain, a histidine kinase domain, and a receiver domain. we have previously demonstrated that, although the erwinia amylovora rcsc mutant produces more amylovoran than the wild-type (wt) strain in vitro, the mutant remains nonpathogenic on both immature pear fruit and apple plants. in this study, we have comparatively characterized the erwinia rcsc and its homologs from various enterobacteria. results demonstrate that expression of the erwi ... | 2011 | 21261468 |
antigenic profiling of yersinia pestis infection in the wyoming coyote (canis latrans). | although yersinia pestis is classified as a "high-virulence" pathogen, some host species are variably susceptible to disease. coyotes (canis latrans) exhibit mild, if any, symptoms during infection, but antibody production occurs postinfection. this immune response has been reported to be against the f1 capsule, although little subsequent characterization has been conducted. to further define the nature of coyote humoral immunity to plague, qualitative serology was conducted to assess the antipl ... | 2011 | 21269993 |
clinical and laboratory findings in 220 children with recurrent abdominal pain. | to investigate the clinical and laboratory findings in children with recurrent abdominal pain (rap). | 2011 | 21272071 |
development of a dna microarray for detection and identification of legionella pneumophila and ten other pathogens in drinking water. | the safety and accessibility of drinking water are major concerns throughout the world. consumption of water contaminated with infectious agents, toxic chemicals or radiological hazards represents a significant health risk and is strongly associated with mortality. therefore, we have developed an oligonucleotide-based microarray using the sequences of 16s-23s rdna internal transcribed spacer regions (its) and the gyrase subunit b gene (gyrb) found in the most prevalent and devastating waterborne ... | 2011 | 21276629 |
quantification of campylobacter spp. in pig feces by direct real-time pcr with an internal control of extraction and amplification. | the rapid and direct quantification of campylobacter spp. in complex substrates like feces or environmental samples is crucial to facilitate epidemiological studies on campylobacter in pig production systems. we developed a real-time pcr assay for detecting and quantifying campylobacter spp. directly in pig feces with the use of an internal control. campylobacter spp. and yersinia ruckeri primers-probes sets were designed and checked for specificity with diverse campylobacter, related organisms, ... | 2011 | 21277341 |
a novel ompy porin from yersinia pseudotuberculosis: structure, channel-forming activity and trimer thermal stability. | a novel ompy porin was predicted based on the yersinia pseudotuberculosis genome analysis. whereas it has the different genomic annotation such as "outer membrane protein n" (abs46310.1) in str. ip 31758 or "outer membrane protein c2, porin" (yp_070481.1) in str. ip32953, it might be warranted to rename the ompn/ompc2 to ompy, "outer membrane protein y", where letter "y" pertained to yersinia. both phylogenetic analysis and genomic localization clearly support that the ompy porin belongs to a ne ... | 2011 | 21142221 |
a dog-associated primary pneumonic plague in qinghai province, china. | primary pneumonic plague (ppp) caused by yersinia pestis is the most threatening clinical form of plague. an outbreak was reported in july 2009 in qinghai province, china. | 2011 | 21288842 |
intracellular yersinia pestis expresses general stress response and tellurite resistance proteins in mouse macrophages. | yersinia pestis inoculated subcutaneously via fleabite or experimental injection in natural rodent hosts multiply initially in macrophage phagolysosomes. survival and multiplication of y. pestis in this acidic low [ca(2+)] and [mg(2+)] environment likely necessitates compensatory mechanisms involving expression of specific proteins compared to those expressed during extracellular growth. a proteomics approach was used to identify these proteins using mouse macrophage raw264.7 cells infected with ... | 2011 | 21295415 |
molecular adaptation of a plant-bacterium outer membrane protease towards plague virulence factor pla. | omptins are a family of outer membrane proteases that have spread by horizontal gene transfer in gram-negative bacteria that infect vertebrates or plants. despite structural similarity, the molecular functions of omptins differ in a manner that reflects the life style of their host bacteria. to simulate the molecular adaptation of omptins, we applied site-specific mutagenesis to make epo of the plant pathogenic erwinia pyrifoliae exhibit virulence-associated functions of its close homolog, the p ... | 2011 | 21310089 |
production of biogenic amines by lactic acid bacteria and enterobacteria isolated from fresh pork sausages packaged in different atmospheres and kept under refrigeration. | the occurrence of in vitro amino acid activity in bacterial strains associated with fresh pork sausages packaged in different atmospheres and kept in refrigeration was studied. the presence of biogenic amines in decarboxylase broth was confirmed by ion-exchange chromatography and by the presence of the corresponding decarboxylase genes by pcr. from the 93 lactic acid bacteria and 100 enterobacteria strains analysed, the decarboxylase medium underestimates the number of biogenic amine-producer st ... | 2011 | 21316866 |
the genetics and structure of the o-specific polysaccharide of yersinia pseudotuberculosis serotype o:10 and its relationship with escherichia coli o111 and salmonella enterica o35. | the o-specific polysaccharide (ops) is a variable constituent of the lipopolysaccharide of gram-negative bacteria. the polymorphic nature of opss within a species is usually first defined serologically, and the current serotyping scheme for yersinia pseudotuberculosis consists of 21 o serotypes of which 15 have been characterized genetically and structurally. here, we present the structure and dna sequence of y. pseudotuberculosis o:10 ops. the o unit consists of one residue each of d-galactopyr ... | 2011 | 21321053 |
dioxygenases in burkholderia ambifaria and yersinia pestis that hydroxylate the outer kdo unit of lipopolysaccharide. | several gram-negative pathogens, including yersinia pestis, burkholderia cepacia, and acinetobacter haemolyticus, synthesize an isosteric analog of 3-deoxy-d-manno-oct-2-ulosonic acid (kdo), known as d-glycero-d-talo-oct-2-ulosonic acid (ko), in which the axial hydrogen atom at the kdo 3-position is replaced with oh. here we report a unique kdo 3-hydroxylase (kdoo) from burkholderia ambifaria and yersinia pestis, encoded by the bamb_0774 (bakdoo) and the y1812 (ypkdoo) genes, respectively. when ... | 2010 | 21178073 |
yersinia pseudotuberculosis mnth functions in intracellular manganese accumulation that is essential for virulence and survival in cells expressing functional nramp1. | manganese has an important yet undefined role in the virulence of many bacterial pathogens. in this study we confirm that a null mutation in yersinia pseudotuberculosis mnth reduces intracellular manganese accumulation. an mnth mutant was susceptible to killing by reactive oxygen species when grown under manganese limited conditions. the mnth mutant was defective in survival and growth in macrophages expressing functional nramp1, but in macrophages deficient in nramp the bacteria were able to su ... | 2010 | 21183572 |
receptor mimicry as novel therapeutic treatment for biothreat agents. | the specter of intentional release of pathogenic microbes and their toxins is a real threat. this article reviews the literature on adhesins of biothreat agents, their interactions with oligosaccharides and the potential for anti-adhesion compounds as an alternative to conventional therapeutics. the minimal binding structure of ricin has been well characterised and offers the best candidate for successful anti-adhesion therapy based on the galβ1-4glcnac structure. the botulinum toxin serotypes a ... | 2010 | 21327124 |
[immunochemical characteristics of synthetic peptides incorporating t- and b-cell epitopes nonspecific porins of pathogenic yersinia]. | multiple antigenic peptides (maps), a sequence which include common antigenic epitopes of outer membrane porins (om) bacteria of the genus yersinia (y. pseudotuberculosis, y. enterocolitica, y. pestis), pathogenic for humans have been synthesized. after immunization of balb/c mice the antiserum to the peptide have been obtained. with the help of elisa we showed that these sera interact with porins isolated from om pathogenic yersinia, and map interact with antibodies in sera from rabbits immuniz ... | 2010 | 21317944 |
oligomeric coiled-coil adhesin yada is a double-edged sword. | yersinia adhesin a (yada) is an essential virulence factor for the food-borne pathogens yersinia enterocolitica and yersinia pseudotuberculosis. surprisingly, it is a pseudogene in yersinia pestis. even more intriguing, the introduction of a functional yada gene in y. pestis ev76 was shown to correlate with a decrease in virulence in a mouse model. here, we report that wild type (wt) y. enterocolitica e40, as well as yada-deprived e40 induced the synthesis of neutrophil extracellular traps (nets ... | 2010 | 21170337 |
distinct ccr2(+) gr1(+) cells control growth of the yersinia pestis δyopm mutant in liver and spleen during systemic plague. | we are using a systemic plague model to identify the cells and pathways that are undermined by the virulence protein yopm of the plague bacterium yersinia pestis. in this study, we pursued previous findings that gr1(+) cells are required to selectively limit growth of δyopm y. pestis and that cd11b(+) cells other than polymorphonuclear leukocytes (pmns) are selectively lost in spleens infected with parent y. pestis. when pmns were ablated from mice, δyopm y. pestis grew as well as the parent str ... | 2010 | 21149593 |
destabilization of yope by the ubiquitin-proteasome pathway fine-tunes yop delivery into host cells and facilitates systemic spread of yersinia enterocolitica in host lymphoid tissue. | pathogenic yersinia species inject a panel of yop virulence proteins by type iii protein secretion into host cells to modulate cellular defense responses. this enables the survival and dissemination of the bacteria in the host lymphoid tissue. we have previously shown that yope of the y. enterocolitica serogroup o8 is degraded in the host cell through the ubiquitin-proteasome pathway. yope normally manipulates rearrangements of the actin cytoskeleton and triggers phagocytosis resistance. to shed ... | 2010 | 21149597 |
functional analysis of vopf activity required for colonization in vibrio cholerae. | vibrio cholerae, a gram-negative facultative pathogen, is the etiologic agent for the diarrheal disease cholera. we previously characterized a clinical isolate, am-19226, that translocates a type iii secretion system (t3ss) effector protein with actin-nucleating activity, vopf, into the host cells. from comparative genomic studies, we identified a divergent t3ss island in additional isolates which possess a vopf homolog, vopn. unlike the vopf-mediated protrusion formation, vopn localizes to stre ... | 2010 | 21151774 |
the yctcba operon of yersinia ruckeri, involved in in vivo citrate uptake, is not required for virulence. | a three-gene operon, named yctcba (yersinia citrate transporter), induced by citrate and repressed by glucose was identified from a previously selected in vivo-induced (ivi) clone in the fish pathogen yersinia ruckeri. interestingly, despite being an ivi clone, the drastic growth reduction of the yctc mutant in the presence of citrate, and the relatively high content of this compound in rainbow trout serum, the operon was not required for virulence. | 2010 | 21131526 |