Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| learning susceptibility of a pathogen to antibiotics using data from similar pathogens. | selection of empirical antibiotic therapy relies on knowledge of the in vitro susceptibilities of potential pathogens to antibiotics. in this paper the limitations of this knowledge are outlined and a method that can reduce some of the problems is developed. | 2009 | 19387503 |
| two chromosomally located qnrb variants, qnrb6 and the new qnrb16, in citrobacter spp. isolates causing bacteraemia. | the objective of this study was to determine the prevalence of the plasmid-borne quinolone resistance genes qnra, qnrb and qnrs in a collection of enterobacteriaceae causing bacteraemia. the presence of the three genes was tested for using multiplex pcr in 306 clinical isolates. plasmid analysis was performed using i-ceui and s1 nuclease digestion and hybridization with specific probes for the qnr and 23s rrna genes. five strains were found to carry a qnr gene, one of which, qnrb16, a new varian ... | 2009 | 19392890 |
| cardiac tamponade caused by polymicrobial gram-negative organisms. | polymicrobial gram-negative pericarditis is a rare entity. we describe the first case of suppurative pericarditis with citrobacter diversus and proteus mirabilis. | 2009 | 19395299 |
| biodegradation potential of pure and mixed bacterial cultures for removal of 4-nitroaniline from textile dye wastewater. | environmentally toxic aromatic amines including nitroanilines are commonly generated in dye contaminated wastewater in which azo dyes undergo degradation under anaerobic conditions. the aim of this study was to develop a process for biological treatment of 4-nitroaniline. three bacteria identified as acinetobacter sp., citrobacter freundii and klebsiella oxytoca were isolated from enrichment cultures of activated sludge on 4-nitroaniline, after which the isolates and the mixed culture were studi ... | 2009 | 19114284 |
| interleukin-17 in host defence against bacterial, mycobacterial and fungal pathogens. | the mammalian immune system is intricately regulated, allowing for potent pathogen-specific immunity to be rapidly activated in response to infection with a broad and diverse array of potential pathogens. as a result of their ability to differentiate into distinct effector lineages, cd4 t cells significantly contribute to pathogen-specific adaptive immune responses. through the production of effector cytokines, cd4 t helper (th) cells orchestrate the precise mobilization of specific immune cells ... | 2009 | 19125888 |
| differential roles of interleukin-17a and -17f in host defense against mucoepithelial bacterial infection and allergic responses. | interleukin-17a (il-17a) is a cytokine produced by t helper 17 (th17) cells and plays important roles in the development of inflammatory diseases. although il-17f is highly homologous to il-17a and binds the same receptor, the functional roles of this molecule remain largely unknown. here, we demonstrated with il17a(-/-), il17f(-/-), and il17a(-/-)il17f(-/-) mice that il-17f played only marginal roles, if at all, in the development of delayed-type and contact hypersensitivities, autoimmune encep ... | 2009 | 19144317 |
| molecular identification of mesophilic and psychrotrophic bacteria from raw cow's milk. | the aim of this study was to use molecular techniques to assess the microbiota of eight raw cow's milk samples at biotype and species level. sixty-six isolates from raw milk samples were screened by randomly amplified polymorphic dna-pcr (rapd-pcr) biotyping and representative strains of rapd-pcr profiles were identified by 16s rrna gene sequencing. pseudomonas spp. were the most commonly occurring contaminants along with enterobacteriaceae such as hafnia alvei, serratia marcescens and citrobact ... | 2009 | 19171267 |
| a prospective, descriptive study to identify the microbiological profile of chronic wounds in outpatients. | indiscriminate use of antibiotics for infected chronic wounds is a global problem that may contribute to delayed healing and the development of drug-resistant micro-organisms. a prospective, descriptive cohort study of 50 male and female outpatients (mean age 52.50 [+/- 14.84] years, range 18-90) with 52 chronic wounds was conducted to investigate the microbiological profile and prevalence of drug-resistant strains in chronic nonhealing wounds to develop an evidence-based approach to antibiotic ... | 2009 | 19174585 |
| modelling of infection by enteropathogenic escherichia coli strains in lineages 2 and 4 ex vivo and in vivo by using citrobacter rodentium expressing tccp. | enteropathogenic escherichia coli (epec) strains colonize the human gut mucosa via attaching-and-effacing (a/e) lesion formation, while in vitro they employ diverse strategies to trigger actin polymerization. strains belonging to the epec-1 lineage trigger strong actin polymerization via tyrosine phosphorylation of the type iii secretion system (t3ss) effector tir, recruitment of nck, and activation of n-wasp. strains belonging to epec-2 and epec-4 can trigger strong actin polymerization by dual ... | 2009 | 19188355 |
| accumulation of plasmid-mediated fluoroquinolone resistance genes, qepa and qnrs1, in enterobacter aerogenes co-producing rmtb and class a beta-lactamase lap-1. | a new plasmid-mediated fluoroquinolone efflux pump gene, qepa, is known to be associated with the rmtb gene, which confers high-level resistance to aminoglycosides. we investigated the qepa gene in 573 ampc-producing enterobacteriaceae including one citrobacter freundii known to harbor rmtb. of them, two clonally unrelated e. aerogenes harbored qepa. both isolates co-harbored rmtb, qnrs1, qepa, and bla(lap-1) on an incfi type plasmid. the qepa was flanked by two copies of is26 containing iscr3c, ... | 2009 | 19201742 |
| prevalence and characteristics of aac(6')-ib-cr in ampc-producing enterobacter cloacae, citrobacter freundii, and serratia marcescens: a multicenter study from korea. | we investigated the prevalence of aac(6')-ib-cr and its association with other resistance genes in ampc-producing enterobacteriaceae without any selection criteria. a total of 479 clinical isolates of enterobacter cloacae (179), citrobacter freundii (134), and serratia marcescens (166) from 12 laboratories between march and july 2005 were examined. we performed polymerase chain reaction for aac(6')-ib, bla(oxa-1), isecp1, and class 1 integron. the aac(6')-ib-cr was further identified by digestio ... | 2009 | 19216942 |
| evaluation of colostrum-derived human mammary-associated serum amyloid a3 (m-saa3) protein and peptide derivatives for the prevention of enteric infection: in vitro and in murine models of intestinal disease. | in vitro experiments confirmed that a 10-mer peptide derived from human mammary-associated serum amyloid a3 (m-saa3) protected intestinal epithelial cells from enteropathogenic escherichia coli (epec) adherence. the entire 42-mer human m-saa3 protein was even more effective, reducing epec binding by 72% relative to untreated cells (p<0.05), compared with 25% and 57% reductions for the human 10-mer and lactobacillus gg, respectively. however, none of the m-saa3 peptides reduced salmonella invasio ... | 2009 | 19220465 |
| [multitalented lymphostatin]. | gastrointestinal infections are a significant cause of diarrhea and a worldwide problem with annually one billion illnesses and 3 to 4 million deaths. gram negative bacteria like enteropathogenic e. coli (epec) in developing countries and enterohemorrhagic e. coli (ehec) in the developed world are responsible for the majority of acute diarrheal episodes, especially among children less than three years of age. pathogenic e. coli are supplied with an arsenal of effector proteins to modify and neut ... | 2009 | 19224428 |
| [a case of peritonitis due to streptococcus gallolyticus subsp. pasteurianus]. | a 55-year-old man admitted on april 1, 2008, for sudden abdominal pain onset whose laboratory data demonstrated apparent inflammation and whose computed tomography (ct) results showed free air and ascites underwent emergency surgery. an ascetic fluid sample submitted for bacteriological examination yielded streptococcus gallolyticus subsp. pasteurianus, escherichia coli, citrobacter freundii, and bacteroides thetaiotaomicron. he was treated for 6 days with flomoxef (fmox; 3 g/day) and recovered, ... | 2009 | 19227226 |
| class 1 and class 2 integrons and plasmid-mediated antibiotic resistance in coliforms isolated from ten rivers in northern turkey. | we aimed to determine the molecular mechanisms of antibiotic resistance in coliforms isolated from ten rivers in northern region of turkey. a total of 183 isolates were tested for antimicrobial susceptibility by disk diffusion and agar dilution methods. resistance to ampicillin, streptomycin, trimethoprim, tetracycline, and chloramphenicol was detected in 58%, 51.9%, 24%, 28.4%, and 12.5%, respectively. twelve (6.5%) phylogenetically distant organisms were detected to harbor self-transmissible p ... | 2009 | 19229487 |
| characterization of a beta-glucoside operon (bgc) prevalent in septicemic and uropathogenic escherichia coli strains. | escherichia coli strains, in general, do not ferment cellobiose and aryl-beta-d-glucosidic sugars, although "cryptic" beta-d-glucoside systems have been characterized. here we describe an additional cryptic operon (bgc) for the utilization of cellobiose and the aryl-beta-d-glucosides arbutin and salicin at low temperature. the bgc operon was identified by the characterization of beta-glucoside-positive mutants of an e. coli septicemia strain (i484) in which the well-studied bgl (aryl-beta-d-gluc ... | 2009 | 19233952 |
| 1h-nuclear magnetic resonance spectroscopy for identifying and quantifying common uropathogens: a metabolic approach to the urinary tract infection. | to address the shortcomings of urine culture for the diagnosis of urinary tract infection (uti), we used 1h-nuclear magnetic resonance (nmr) spectroscopy for identifying and quantifying escherichia coli, pseudomonas aeruginosa, klebsiella pneumonia and proteus mirabilis. | 2009 | 19239442 |
| contribution of the pst-phou operon to cell adherence by atypical enteropathogenic escherichia coli and virulence of citrobacter rodentium. | strains of enteropathogenic escherichia coli (epec) generally employ the adhesins bundle-forming pili (bfp) and intimin to colonize the intestine. atypical epec strains possess intimin but are negative for bfp and, yet, are able to cause disease. to identify alternative adhesins to bfp in atypical epec, we constructed a transposon mutant library of atypical epec strain e128012 (serotype o114:h2) using tnphoa. six mutants that had lost the ability to adhere to hep-2 cells were identified, and in ... | 2009 | 19255191 |
| detection of bacterioplankton in immersed cadavers using selective agar plates. | we measured bacterioplankton in blood from cadavers retrieved from the sea (n=12), near estuaries (n=4), rivers (fresh water, n=8) and from bathtubs (n=4) as well as from non-drowned victims (n=10) discovered near aquatic environments. blood from 11 victims drowned in seawater developed bioluminescent and/or blue colonies (oxidase test positive) on selective media containing 2-4% nacl. homology analyses of the 16s rrna gene showed that all of them were marine bacteria (genera: photobacterium, vi ... | 2009 | 19261520 |
| effect of probiotics lactobacillus acidophilus on citrobacter rodentium colitis: the role of dendritic cells. | modulation of the intestinal immune response early in life by administration of probiotic bacteria may be an effective strategy for preventing or attenuating infectious diarrhea. we preinoculated the mice early in life with the probiotic bacteria lactobacillus acidophilus ncfm (la) at age 2 wk. dendritic cells (dcs) were collected and purified from mesenteric lymph nodes (mln) and spleens of the balbc/byj mice. dc isolation and adoptive transfer was used to examine the function of probiotics. we ... | 2009 | 19262293 |
| innate immune recognition of infected apoptotic cells directs t(h)17 cell differentiation. | adaptive immune responses rely on differentiation of cd4 t helper cells into subsets with distinct effector functions best suited for host defence against the invading pathogen. interleukin (il)-17-producing t helper cells (t(h)17) are a recently identified subset, separate from the t helper type 1 (t(h)1) and t helper type 2 (t(h)2) subsets. synergy between the cytokines transforming growth factor-beta and il-6 in vitro induces development of t(h)17 cells in mouse and human systems, whereas il- ... | 2009 | 19262671 |
| the bacterial virulence factor lymphostatin compromises intestinal epithelial barrier function by modulating rho gtpases. | lymphocyte inhibitory factor a (lifa) in citrobacter rodentium encodes the large toxin lymphostatin, which contains two enzymatic motifs associated with bacterial pathogenesis, a glucosyltransferase and a protease. our aim was to determine the effects of each lymphostatin motif on intestinal epithelial-barrier function. in-frame mutations of c. rodentium lifa glucosyltransferase (crglm21) and protease (crprm5) were generated by homologous recombination. infection of both model intestinal epithel ... | 2009 | 19286565 |
| antibiotic susceptibility pattern of staphylococcus species isolated from telephone receivers. | microorganisms are transferred to everyday objects from the environment and infected individuals. pathogenic microbes are transmissible from the air, skin, hands and other interpersonal contacts and cause diseases most of the time. this study centres on the microbial assessment of telephone receivers, their ability to transfer bacterial infections and the isolation of these microorganisms from receivers, with an emphasis on staphylococcus aureus and its antibiotic susceptibility pattern. | 2009 | 19296038 |
| emergence of a plasmid mediated bla (vim-1) in citrobacter koseri: report from the sentry antimicrobial surveillance program (italy). | 2009 | 19297281 | |
| novel ctx-m {beta}-lactamase genotype distribution and spread into multiple species of enterobacteriaceae in changsha, southern china. | the aim of this study was to undertake a survey of the occurrence of ctx-m and shv extended-spectrum beta-lactamase (esbl) genotypes in enterobacteriaceae from hunan province, china. | 2009 | 19297379 |
| leptoglycin: a new glycine/leucine-rich antimicrobial peptide isolated from the skin secretion of the south american frog leptodactylus pentadactylus (leptodactylidae). | antimicrobial peptides are components of innate immunity that is the first-line defense against invading pathogens for a wide range of organisms. here, we describe the isolation, biological characterization and amino acid sequencing of a novel neutral glycine/leucine-rich antimicrobial peptide from skin secretion of leptodactylus pentadactylus named leptoglycin. the amino acid sequence of the peptide purified by rp-hplc (c(18) column) was deduced by mass spectrometric de novo sequencing and conf ... | 2009 | 19298834 |
| effects of an alternative cefepime dosing strategy in pulmonary and bloodstream infections caused by enterobacter spp, citrobacter freundii, and pseudomonas aeruginosa: a single-center, open-label, prospective, observational study. | various dosing strategies for cefepime have been developed in an effort to maximize pharmacodynamic exposure of this agent against gram-negative infections. an assessment of cefepime dosing strategies is warranted given recent reports of poorer treatment outcomes associated with cefepime compared with other antibiotics, particularly in patients infected with gram-negative organisms with elevated mics. | 2009 | 19302902 |
| targeted capture of pathogenic bacteria using a mammalian cell receptor coupled with dielectrophoresis on a biochip. | efficient capture of target analyte on biosensor platforms is a prerequisite for reliable and specific detection of pathogenic microorganisms in a microfluidic chip. antibodies have been widely used as ligands; however, because of their occasional unsatisfactory performance, a search for alternative receptors is underway. heat shock protein 60 (hsp60), a eukaryotic mitochondrial chaperon protein is a receptor for listeria adhesion protein (lap) during listeria monocytogenes infection. this paper ... | 2009 | 19317455 |
| genomic analysis of the pai icl3 locus in pathogenic lee-negative shiga toxin-producing escherichia coli and citrobacter rodentium. | shiga toxin-producing escherichia coli (stec) causes a spectrum of human illnesses such as haemorrhagic colitis and haemolytic-uraemic syndrome. although the locus of enterocyte effacement (lee) seems to confer enhanced virulence, lee-negative stec strains are also associated with severe human disease, suggesting that other unknown factors enhance the virulence potential of stec strains. a novel hybrid pathogenicity island, termed pai i(cl3), has been previously characterized in the lee-negative ... | 2009 | 19332804 |
| molecular characterisation of the reca locus in clinical isolates of verocytotoxigenic e. coli o157:h7. | molecular epidemiology of verocytoxigenic escherichia coli o157:h7 is important to help elucidate reservoirs and modes of transmission, particularly between animals and humans. as the reca gene locus is now beginning to gain application in bacterial genotyping schemes, and as it has not been examined previously in e. coli o157 isolates, this study aims to examine potential polymorphic variation as a possible epidemiological marker for the subspecies characterisation of clinically significant ver ... | 2009 | 19348125 |
| [ability of opportunistic enterobacteria to adapt to different temperatures]. | to study variability of enzymatic apparatus of opportunistic enterobacteria. | 2009 | 19459471 |
| citrobacter rodentium colitis evokes post-infectious hyperexcitability of mouse nociceptive colonic dorsal root ganglion neurons. | to investigate the possible contribution of peripheral sensory mechanisms to abdominal pain following infectious colitis, we examined whether the citrobacter rodentium mouse model of human e. coli infection caused hyperexcitability of nociceptive colonic dorsal root ganglion (drg) neurons and whether these changes persisted following recovery from infection. mice were gavaged with c. rodentium or distilled water. perforated patch clamp recordings were obtained from acutely dissociated fast blue ... | 2009 | 19470777 |
| antibiotic resistance of gram negatives isolates from loggerhead sea turtles (caretta caretta) in the central mediterranean sea. | previous studies on fish and marine mammals support the hypothesis that marine species harbor antibiotic resistance and therefore may serve as reservoirs for antibiotic-resistance genetic determinants. the aim of this study was to assess the resistance to antimicrobial agents of gram negative strains isolated from loggerhead sea turtles (carettacaretta). oral and cloacal swabs from 19 live-stranded loggerhead sea turtles, with hooks fixed into the gut, were analyzed. the antimicrobial resistance ... | 2009 | 19473669 |
| temporal alterations in brain water diffusivity in neonatal meningitis. | to compare changes in apparent diffusion coefficient (adc) in neonatal meningitis using serial diffusion-weighted imaging (dwi). | 2009 | 19485957 |
| bacteria hijack integrin-linked kinase to stabilize focal adhesions and block cell detachment. | the rapid turnover and exfoliation of mucosal epithelial cells provides an innate defence system against bacterial infection. nevertheless, many pathogenic bacteria, including shigella, are able to surmount exfoliation and colonize the epithelium efficiently. here we show that the shigella flexneri effector ospe (consisting of ospe1 and ospe2 proteins), which is highly conserved among enteropathogenic escherichia coli, enterohaemorrhagic e. coli, citrobacter rodentium and salmonella strains, rei ... | 2009 | 19489119 |
| bacterial flora in normal adult maxillary sinuses. | conflicting data exist about the presence of bacteria in healthy maxillary sinus cavities. this study was designed to determine the bacterial flora and to quantify the level of bacterial presence in healthy maxillary sinus cavities. | 2009 | 19490798 |
| bacterial ampd at the crossroads of peptidoglycan recycling and manifestation of antibiotic resistance. | the bacterial enzyme ampd is an early catalyst in commitment of cell wall metabolites to the recycling events within the cytoplasm. the key internalized metabolite of cell wall recycling, beta-d-n-acetylglucosamine-(1-->4)-1,6-anhydro-beta-n-acetylmuramyl-l-ala-gamma-d-glu-meso-dap-d-ala-d-ala (compound 1), is a poor substrate for ampd. two additional metabolites, 1,6-anhydro-n-acetylmuramyl-peptidyl derivatives 2a and 2c, served as substrates for ampd with a k(cat)/k(m) of >10(4) m(-1) s(-1). t ... | 2009 | 19496566 |
| activity of the anti-mrsa carbapenem razupenem (ptz601) against enterobacteriaceae with defined resistance mechanisms. | razupenem (previously known as ptz601, pz-601, smp-601 or sm-216601) is a novel carbapenem, active against enterobacteriaceae as well as gram-positive bacteria including methicillin-resistant staphylococci and enterococci. | 2009 | 19497942 |
| methionine gamma-lyase: mechanistic deductions from the kinetic ph-effects. the role of the ionic state of a substrate in the enzymatic activity. | we have studied and compared the ph-dependencies of the main kinetic parameters for the alpha,gamma-elimination reactions of methionine gamma-lyase (mgl) of citrobacter intermedius with natural substrate, l-methionine, with its phosphinic analogue, and for alpha,beta-elimination reaction with s-methyl-l-cysteine. from the ph-dependency of k(cat)/k(m) for the reaction with l-methionine we have concluded that mgl is selective with respect to the zwitterionic form of its natural substrate. for the ... | 2009 | 19501676 |
| specificity analysis of a novel phage-derived ligand in an enzyme-linked fluorescent assay for the detection of escherichia coli o157:h7. | an assay using a phage-derived ligand to capture escherichia coli o157:h7 prior to antibody detection was evaluated for assay specificity. analysis of 200 strains showed that the assay was highly specific for the o157 serogroup. it detected all the o157:h7 strains including shiga toxin-producing o157 nonmotile strains as well as o157 non-h7 strains. in addition, the assay detected various o157:h7 phenotypic variants that are not easily detected by routine analytical methods, as well as a rough s ... | 2009 | 19517737 |
| distribution of espm and espt among enteropathogenic and enterohaemorrhagic escherichia coli. | enterohaemorrhagic escherichia coli (ehec) and enteropathogenic e. coli (epec) translocate dozens of type iii secretion system effectors, including the wxxxe effectors map, espm and espt that activate rho gtpases. while map, which is carried on the lee pathogenicity island, is absolutely conserved among epec and ehec strains, the prevalence of espm and espt is not known. here we report the results of a large screen aimed at determining the prevalence of espm and espt among clinical epec and ehec ... | 2009 | 19528152 |
| decreased expression of colonic slc26a3 and carbonic anhydrase iv as a cause of fatal infectious diarrhea in mice. | citrobacter rodentium causes epithelial hyperplasia and colitis and is used as a model for enteropathogenic and enterohemorrhagic escherichia coli infections. little or no mortality develops in most inbred strains of mice, but c3h and fvb/n mice exhibit fatal outcomes of infection. here we test the hypothesis that decreased intestinal transport activity during c. rodentium infection results in fatality in c3h/heou and fvb/n mice. susceptible strains were compared to resistant c57bl/6 mice and to ... | 2009 | 19546193 |
| pathogenic bacteria induce colonic pept1 expression: an implication in host defense response. | expression of the di/tripeptide transporter pept1 has been observed in the colon under inflammatory conditions; however, the inducing factors and underlying mechanisms remain unknown. here, we address the effects of pathogenic bacteria on colonic pept1 expression together with its functional consequences. | 2009 | 19549526 |
| antibiotic susceptibility profiles of uncommon bacterial species causing severe infections in italy. | this study presents the results of the italian "severe infections project" involving bacteria that can be considered rare causes of disease. we isolated 30 uncommon human pathogens from a total of 60 strains (1.2% of all the isolates). the most frequent sources of uncommon human pathogens were primary bloodstream infections (48.3%) and pneumonia (20%). species such as comamonas testosteroni, enterococcus hirae, kluyvera ascorbata, kluyvera cryocrescens, leclercia adecarboxylata and ochrobactrum ... | 2009 | 19567344 |
| structure of an abequose-containing o-polysaccharide from citrobacter freundii o22 strain pcm 1555. | the lipopolysaccharide of citrobacter freundii o22 (strain pcm 1555) was degraded under mild acidic conditions and the o-polysaccharide released was isolated by gel chromatography. sugar and methylation analyses along with (1)h and (13)c nmr spectroscopy, including two-dimensional (1)h,(1)h roesy and (1)h,(13)c hmbc experiments, showed that the repeating unit of the o-polysaccharide has the following structure: alpha-abep 1 -->3 --> 2)-alpha-d-manp-(1-->4)-alpha-l-rhap-(1-->3)-alpha-d-galp-(1--> ... | 2009 | 19576576 |
| production of l-dopa and dopamine in recombinant bacteria bearing the vitreoscilla hemoglobin gene. | given the well-established beneficial effects of vitreoscilla hemoglobin (vhb) on heterologous organisms, the potential of this protein for the production of l-dopa and dopamine in two bacteria, citrobacter freundii and erwinia herbicola, was investigated. the constructed recombinants bearing the vhb gene (vgb(+)) had substantially higher levels of cytoplasmic l-dopa (112 mg/l for c. freundii and 97 mg/l for e. herbicola) than their respective hosts (30.4 and 33.8 mg/l) and the vgb(-) control st ... | 2009 | 19585534 |
| influence of long-term consumption of a lactococcus lactis strain on the intestinal immunity and intestinal flora of the senescence-accelerated mouse. | the senescence-accelerated mouse develops normally until 5-6 months of age and then displays rapid and irreversible advancement of senescence manifesting as clinical signs and gross lesions. to clarify the effect of lactic acid bacteria on the physiological changes with increasing age, heat-killed lactococcus lactis g50 was administered to 1-month-old senescence-accelerated-prone mouse (samp)6 mice for 11 months, a senescence-accelerated mouse strain that develops senile osteoporosis. mice fed g ... | 2009 | 19586567 |
| genetic context of plasmid-carried blacmy-2-like genes in enterobacteriaceae. | analysis of 15 european clinical enterobacteriaceae isolates showed that differences in the genetic context of blacmy-2-like genes reflected the replicon type, usually inca/c or inci1. these blacmy-2 loci may originate from the same isecp1-mediated mobilization from the citrobacter freundii chromosome as structures described in earlier studies. | 2009 | 19596889 |
| synthesis, characterization and evaluation of antibacterial activity of some thiazolo[3,2-b][1,2,4]triazole incorporating diphenylsulfone moieties. | a series of thiazolo[3,2-b][1,2,4]triazole incorporating diphenylsulfone moieties were synthesized starting from 5-[4-(4-x-phenylsulfonyl)phenyl]-4h-1,2,4-triazole-3-thioles 3a-c, x=h, cl, br. thus, alkylation of 1,2,4-triazoles 3 with phenacyl bromide or 4-bromophenacyl bromide afforded s-substituted 1,2,4-triazoles 4, 5. these new intermediates 4 and 5, in the presence of h(2)so(4) (c), were cyclized to 2-[4-(4-x-phenylsulfonyl)phenyl]-6-(4-y-phenyl)[1,3]thiazolo[3,2-b]-[1,2,4]-triazoles 6, 7 ... | 2009 | 19616347 |
| bacterial contamination of blood and blood components in three major blood transfusion centers, accra, ghana. | reports from studies conducted in several countries indicate a high incidence of bacterial contamination of donor blood. the prevalence of bacterial contamination of blood and its products in ghana is not known. this study was conducted to determine the prevalence of bacterial contamination of blood and its products at the three major blood transfusion centers in the greater accra region of ghana. stored whole blood and its products were cultured on different media, and isolates were identified ... | 2009 | 19628902 |
| differentiation of whole bacterial cells based on high-throughput microarray chip printing and infrared microspectroscopic readout. | using robotic automation, a microarray printing protocol for whole bacterial cells was developed for subsequent label-free and nondestructive infrared microspectroscopic detection. using this contact microspotting system, 24 microorganisms were printed on zinc selenide slides; these were 6 species of listeria, 10 species of vibrio, 2 strains of photobacterium damselae, yersinia enterocolitica 289, bacillus cereus atcc 14529, staphylococcus aureus, atcc 19075 (serotype 104 b), shigella sonnei 201 ... | 2009 | 19630511 |
| community-wide response of the gut microbiota to enteropathogenic citrobacter rodentium infection revealed by deep sequencing. | we investigated the spatial and temporal response of the murine gut microbiome to infection with citrobacter rodentium, an attaching-and-effacing bacterium that provokes innate and adaptive immune responses, resulting in transient bacterial colitis. previous studies have suggested that c. rodentium-induced inflammation is associated with an increased abundance of enterobacteriaceae. we report here a deeper analysis of this model using dna bar coding and 454 pyrosequencing to characterize 101,894 ... | 2009 | 19635824 |
| resistance against broad-spectrum beta-lactams among uropathogens in children. | the aim of this study was to investigate the prevalence trends and risk factors for urinary tract infection (uti) caused by enterobacteriaceae resistant to broad-spectrum beta-lactams in children. all enterobacteriaceae uropathogens from children <15 years during the 11-year period 1997-2007 were included, and risk factors were evaluated. of 523 enterobacteriaceae isolated from 473 children, 30 (5.73%) were phenotypically resistant to broad-spectrum beta-lactams (18 escherichia coli, ten klebsie ... | 2009 | 19636594 |
| phenotypic characterization of human pathogenic bacteria in fish from the coastal waters of south west cameroon: public health implications. | increasing economic and recreational opportunities, attractive scenery and a perception of a better quality of life are luring people to the coast. unfortunately, these activities together with the commensurate increase in population in the area inevitably result in pollution of coastal waters with excessive microorganisms and other pollutants. microbial pollutants not only contaminate the coastal water but also aquatic food sources, thus posing a health risk to consumers. fish is a major source ... | 2009 | 19658320 |
| involvement of t helper type 17 and regulatory t cell activity in citrobacter rodentium invasion and inflammatory damage. | citrobacter rodentium is a murine pathogen that transiently colonizes the lumen of the large intestine. c. rodentium induces colitis, but the relative importance and temporal induction of the t helper type 17 (th17) and regulatory t cell (t(reg)) pathways in protection from the infection and inflammation have not been assessed. our aim was to investigate the key immunological signalling events associated with successful clearance of c. rodentium. mice were challenged with luminescent-tagged c. r ... | 2009 | 19659780 |
| vasoactive intestinal peptide ameliorates intestinal barrier disruption associated with citrobacter rodentium-induced colitis. | attaching and effacing bacterial pathogens attach to the apical surface of epithelial cells and disrupt epithelial barrier function, increasing permeability and allowing luminal contents access to the underlying milieu. previous in vitro studies demonstrated that the neuropeptide vasoactive intestinal peptide (vip) regulates epithelial paracellular permeability, and the high concentrations and close proximity of vip-containing nerve fibers to intestinal epithelial cells would support such a func ... | 2009 | 19661153 |
| screening, purification, and identification of the enzyme producing n-(l-alpha-l-aspartyl)-l-phenylalanine methyl ester from l-isoasparagine and l-phenylalanine methyl ester. | screening was carried out for microorganisms able to produce n-(l-alpha-l-aspartyl)-l-phenylalanine methyl ester [apm] from l-isoasparagine and l-phenylalanine methyl ester hydrochloride. of the 422 strains examined, 44 strains belonging to the family enterobacteriaceae were found to produce apm. the enzyme catalyzing apm production was purified and identified as dipeptidase e. | 2009 | 19664550 |
| th17 cytokines and host-pathogen interactions at the mucosa: dichotomies of help and harm. | the mucosal surfaces are often the first site of interaction between pathogenic microorganisms and the host. activation of the mucosal immune response has the important function of containing an infection and preventing dissemination of pathogens to systemic sites (barrier function). numerous lines of evidence suggest that the barrier function is orchestrated by a subset of cytokines (interleukin (il-)17 and il-22), which belong to the th17 family. il-17 and il-22 induce expression of antimicrob ... | 2009 | 19665391 |
| [antimicrobial activity of tebipenem against various clinical isolates from various specimen, mainly urinary tract]. | tebipenem is the active metabolite of me1211, tebipenem pivoxil, a novel oral carbapenem that possesses potent activity against almost pathogens except for pseudomonas aeruginosa. in this study, we compared the susceptibility of tebipenem with current antibiotics against various organisms isolated from various specimen, mainly urinary tract. tebipenem had a potent activity against neisseria gonorrhoeae; its activity was comparable to it of cefixime that has most potent activity among oral antibi ... | 2009 | 19673353 |
| cxcr2-dependent mucosal neutrophil influx protects against colitis-associated diarrhea caused by an attaching/effacing lesion-forming bacterial pathogen. | enteropathogenic escherichia coli (epec) is a major cause of diarrheal disease in young children, yet symptoms and duration are highly variable for unknown reasons. citrobacter rodentium, a murine model pathogen that shares important functional features with epec, colonizes mice in colon and cecum and causes inflammation, but typically little or no diarrhea. we conducted genome-wide microarray studies to define mechanisms of host defense and disease in c. rodentium infection. a significant fract ... | 2009 | 19675161 |
| hidden qnrb12 gene in a finnish faecal microbiota isolate from 1994. | 2009 | 19692413 | |
| bacteria isolated from 25 hydatid cysts in sheep, cattle and goats. | bacteria were isolated from 12 of 25 hydatid cysts collected from the lungs and livers of cattle, sheep and goats slaughtered in the province of messina, sicily, italy. citrobacter freundii was isolated from seven of the cysts, aeromonas hydrophila from three, staphylococcus species from two, salmonella species from two and escherichia coli and proteus vulgaris from one. | 2009 | 19700784 |
| comparative study of anthocyanin composition, antimicrobial and antioxidant activity in bilberry (vaccinium myrtillus l.) and blueberry (vaccinium corymbosum l.) fruits. | simultaneous comparison of bilberry (vaccinium myrtillus l.) and blueberry (vaccinium corymbosum l) fruits for their anthocyanin composition, antimicrobial and antioxidant activity is reported. the aim of this study was to investigate and to compare anthocyanin composition, antimicrobial and antioxidant activity in bilberry and blueberry fruits and their skins. the investigations revealed that the highest amount of total anthocyanins was observed in fruits skins of blueberry cultivars. the resul ... | 2009 | 19702172 |
| an outer membrane protease of the omptin family prevents activation of the citrobacter rodentium phopq two-component system by antimicrobial peptides. | the phopq two-component system of the intracellular pathogen salmonella enterica senses and controls resistance to alpha-helical antimicrobial peptides (amps) by regulating covalent modifications of lipid a. a homologue of the phopq operon was found in the genome of the murine enteric extracellular pathogen, citrobacter rodentium. here we report that c. rodentium phopq was apparently unable to mediate activation of target genes in the presence of alpha-helical amps. however, these amps activated ... | 2009 | 19708916 |
| [formation of biofilms by fecal strains of enterobacteria and yeast fungi from candida genus]. | to assess the ability of bacteria and candida genus' yeast fungi from human fecal microflora to form biofilms. | 2009 | 19715202 |
| study of the bacterial ecosystem in tropical cooked and peeled shrimps using a polyphasic approach. | the characterization of the microbial ecosystem of cooked tropical shrimps was carried out using a polyphasic approach. first, culture-dependent methods were used for bacterial enumeration and the phenotypic and molecular identification of bacterial isolates. then, culture-independent methods, including pcr-ttge (v3 region of the 16s rrna gene), provided a fingerprinting of bacterial dna directly extracted from shrimps. two batches of cooked and peeled tropical shrimps were stored at 5 and 15 de ... | 2009 | 18573557 |
| synthesis, characterization and antibacterial activity of some triazole mannich bases carrying diphenylsulfone moieties. | a series of mannich bases of 4-substituted 5-[4-(4-x-phenylsulfonyl)phenyl]-2,4-dihydro-3h-1,2,4-triazole-3-thiones, x=h, cl, br, 3 and 5 were synthesized and characterized on the basis of ir, nmr and elemental analyses. the potential antibacterial effects of the synthesized compounds were investigated using the acinetobacter baumanii atcc 19606; citrobacter freundii atcc 8090; pseudomonas aeruginosa atcc 9027; enterococcus faecalis atcc 19433; staphylococcus aureus atcc 12600; staphylococcus ep ... | 2009 | 18708273 |
| citrobacter infections in a general hospital: characteristics and outcomes. | in this investigation, we sought to investigate the characteristics of citrobacter spp. infections. a retrospective cohort study in a 700-bed, tertiary care, university hospital was carried out during the period from june 1994 to january 2006. seventy-eight patients (70 adults) with citrobacter spp. isolates were identified. c. freundii was more common (71.8%), followed by c. koseri (23.1%) and c. braakii (3.8%). the most common associated clinical syndromes were urinary tract infections (52.6% ... | 2009 | 18682995 |
| ecthyma gangrenosum with citrobacter freundii infection. | 2009 | 18759794 | |
| physicochemical treatments of anionic surfactants wastewater: effect on aerobic biodegradability. | the effect of different physicochemical treatments on the aerobic biodegradability of an industrial wastewater resulting from a cosmetic industry has been investigated. this industrial wastewater contains 11423 and 3148mgl(-1) of chemical oxygen demand (cod) and anionic surfactants, respectively. the concentration of cod and anionic surfactants were followed throughout the diverse physicochemical treatments and biodegradation experiments. different pretreatments of this industrial wastewater usi ... | 2009 | 18799262 |
| [emergence of vancomycin-dependent enterococci following glycopeptide therapy: case report and review]. | outbreaks of vancomycin-resistant enterococci have been increasingly reported in france over the last three years. we report here, the emergence of a vancomycin-dependent enterococci isolate following glycopeptide therapy. | 2009 | 18845404 |
| regulation of hepatic cytochrome p450 expression in mice with intestinal or systemic infections of citrobacter rodentium. | we reported previously that infection of c3h/heouj (heou) mice with the murine intestinal pathogen citrobacter rodentium caused a selective modulation of hepatic cytochrome p450 (p450) gene expression in the liver that was independent of the toll-like receptor 4. however, heou mice are much more sensitive to the pathogenic effects of c. rodentium infection, and the p450 down-regulation was associated with significant morbidity in the animals. here, we report that oral infection of c57bl/6 mice w ... | 2009 | 18971315 |
| a survey on species and prevalence rate of bacterial agents isolated from cockroaches in three hospitals. | the presence of cockroaches has health implications, such as nosocomial infection, as the insects move freely from areas within and around hospitals that may harbor pathogenic organisms. the goals of the present study were to determine species of bacteria isolated from cockroaches found in three tehran hospitals, and to determine distribution of potential vectors by species and sex. the study is descriptive laboratory research. a total of 305 cockroaches from three species were trapped and ident ... | 2009 | 18973441 |
| sulfate reduction and copper precipitation by a citrobacter sp. isolated from a mining area. | a strain of sulfate-reducing bacteria, designated strain 'dbm', was isolated from sediments of a mining area. phylogenetic analysis of the 16s rrna gene sequence of the isolate revealed that it was related to members of the genus citrobacter, with c. azor-4, c. freundii, c. braakii and c. werkmanii being the most closely related species (sequence similarity up to 98%). few studies have been done on sulfate reduction ability in citrobacter. electron microscopy studies showed that the morphology o ... | 2009 | 18977087 |
| role of rpos in the virulence of citrobacter rodentium. | citrobacter rodentium is a mouse enteropathogen that is closely related to escherichia coli and causes severe colonic hyperplasia and bloody diarrhea. c. rodentium infection requires expression of genes of the locus of enterocyte effacement (lee) pathogenicity island, which simulates infection by enteropathogenic e. coli and enterohemorrhagic e. coli in the human intestine, providing an effective model for studying enteropathogenesis. in this study we investigated the role of rpos, the stationar ... | 2009 | 18981255 |
| optimization of process parameters for the production of tyrosine phenol lyase by citrobacterfreundii mtcc 2424. | the process optimization using technological combinations for the production of tyrosine phenol lyase by citrobacter freundii mtcc 2424 has been carried out in this study. the maximum production of tyrosine phenol lyase (0.15 u) was obtained by culturing c. freundii mtcc 2424 in a medium containing (g/l) meat extract 5.0, yeast extract 5.0, peptone 2.5, and l-tyrosine 1.0 at 25 degrees c for 16 h in a temperature controlled orbital shaker. a 2.5-fold increase in enzyme activity with 1.3-fold dec ... | 2009 | 18993058 |
| beta-catenin stabilization imparts crypt progenitor phenotype to hyperproliferating colonic epithelia. | utilizing the citrobacter rodentium (cr)-induced transmissible murine colonic hyperplasia (tmch) model, we provide mechanistic basis of changes in beta-catenin/apc/ckiepsilon leading to progression and/or regression of hyperplasia in vivo. in response to cr-induced tmch, crypt lengths increased significantly between days 6-27 post-infection, followed by a steep decline by day 34. beta-cat(45)/total beta-catenin were elevated on day 1 post-infection, preceding changes in crypt length, and persist ... | 2009 | 18996369 |
| espt triggers formation of lamellipodia and membrane ruffles through activation of rac-1 and cdc42. | subversion of the eukaryotic cell cytoskeleton is a virulence strategy employed by many bacterial pathogens. due to the pivotal role of rho gtpases in actin dynamics they are common targets of bacterial effector proteins and toxins. ipgb1, ipgb2 (shigella), sifa, sifb (salmonella) and map and espm (attaching and effacing pathogens) constitute a family of type iii secretion system effectors that subverts small gtpase signalling pathways. in this study we identified and characterized espt from cit ... | 2009 | 19016787 |
| from kinase to cyclase: an unusual example of catalytic promiscuity modulated by metal switching. | 2009 | 19058275 | |
| interleukin-1 receptor signaling protects mice from lethal intestinal damage caused by the attaching and effacing pathogen citrobacter rodentium. | enteropathogenic escherichia coli, enterohemorrhagic e. coli, and citrobacter rodentium are classified as attaching and effacing pathogens based on their ability to adhere to the intestinal epithelium via actin-filled membranous protrusions (pedestals). infection of mice with c. rodentium causes a breach of the intestinal epithelial barrier, leading to colitis via a vigorous inflammatory response resulting in diarrhea and a protective antibody response that clears the pathogen. here we show that ... | 2009 | 19075023 |
| hepatic flavin-containing monooxygenase gene regulation in different mouse inflammation models. | the objective of the study was to investigate the regulation of hepatic flavin-containing monooxygenases (fmo) fmo1, fmo3, fmo4, and fmo5 in three different mouse models of inflammation, including treatment with citrobacter rodentium, lipopolysaccharide (lps), and dextran sulfate sodium (dss). quantitative real-time reverse transcription-polymerase chain reaction (rt-pcr) was used to evaluate the steady-state mrna levels for the various fmo isoforms in these mouse models of inflammation during d ... | 2009 | 19088265 |
| cmy-31 and cmy-36 cephalosporinases encoded by cole1-like plasmids. | two cmy-2 derivatives, cmy-31 (gln(215)-->arg) from salmonella enterica serotype newport and cmy-36 (ala(77)-->cys and gln(193)-->glu) from klebsiella pneumoniae, were characterized. both cephalosporinases functionally resembled cmy-2. bla(cmy) alleles occurred as parts of a putative transposon comprising isecp1b and a citrobacter freundii-derived sequence carried by cole1-like plasmids similar to cmy-5-encoding ptkh11 from klebsiella oxytoca. | 2009 | 19104021 |
| dark secrets behind the shimmer of contact lens: the indian scenario. | we studied the bacteriological profile of soft contact lens and its accessories among the asymptomatic subjects and monitored the compliance level with the lens use and its cleaning protocol. | 2009 | 19426534 |
| determination of bacterial antibiotic resistance based on osmotic shock response. | we investigate the buoyant mass of bacterial cells in real time with the suspended microchannel resonator (smr) as the population recovers from an osmotic shock. the density of the culture medium is chosen such that the bacteria initially have a positive buoyant mass which becomes negative as they recover from the hyperosmotic stress. this behavior can be used to differentiate between an antibiotic-resistant and an antibiotic-susceptible strain of the pathogenic bacteria citrobacter rodentium, a ... | 2009 | 20337387 |
| multidrug-resistant nosocomial citrobacter in a hospital in kathmandu. | citrobacter sp. is a commensal flora and an infrequent nosocomial pathogen to cause nuisance in hospital settings. recently, the isolation of this pathogen in health care setting is rising and the multidrug resistant strains are emerging. this pathogen cause wide array of infections and the mortality rate is unexpectedly high of 30.0-60.0%. extended spectrum cephalosporins have been used to treat this pathogen and due to the emergence of resistant strains to these drugs newer treatment protocols ... | 2009 | 20334070 |
| [enteropathogens relating to diarrhea in hiv patients on antiretroviral therapy]. | the etiology of the diarrheic process in aids may be caused by viruses, bacteria, fungi, protozoa or helminths, as well as hiv itself. this study evaluated enteropathogens relating to diarrhea in hiv patients who were on antiretroviral therapy. the parasitological methods used were faust, hoffmann and kinyoun. isolation and culturing of fungi were carried out in accordance with the methodology recommended by the nccls m27-a standard. the yeast species were identified using the polymerase chain r ... | 2009 | 19967238 |
| extended spectrum beta-lactamase detection in gram-negative bacilli of nosocomial origin. | resistance to third generation cephalosporins by acquisition and expression of extended spectrum beta lactamase (esbl) enzymes among gram-negative bacilli is on a rise. the presence of esbl producing organisms significantly affects the course and outcome of an infection and poses a challenge to infection management worldwide. | 2009 | 20300397 |
| plasmid incidence, antibiotic and metal resistance among enterobacteriaceae isolated from algerian streams. | enterobacteriaceae isolates from surface water were examined to assess impact of feacal and/or metal pollution on heavy metal, antibiotics resistance and plasmid incidence. a bi-modal cmi distribution was noted for cadmium and mercury. on the other hand, modal distribution was observed for pb. critical metal concentration were >8, >32, > or =4096 microg ml(-1) for mercury, cadmium and lead, respectively. high resistance to pb and low resistance to cd were remarked in stream water polluted with h ... | 2009 | 20180322 |
| resistances of antibiotics and heavy metals in enterobacteriaceae spp. isolated from gills and intestines of achanthobrama marmid (heckel,1843) from sir dam lake turkey. | a total of 94 bacteria, associated with wild achanthobrama marmid (heckel, 1843) in sir dam lake of turkey identified. subsequently selected isolates were characterized and identified to the genus level the 94 members of enterobacteriaceae were isolated in the gills and intestines, and among the isolates, e. coli were represented at a rate of 55%, shigella spp. at a rate of 21%, salmonella spp. at a rate of 9%, citrobacter spp. at a rate of 9%, klebsiella spp. at a rate of 3% and proteus spp., a ... | 2009 | 20112859 |
| [citrobacter koseri brain abscess in an infant: case report and literature review]. | we present a 2 month-old female infant admitted to the hospital with macrocephaly as main clinical sign, irritability, and vomiting. the computer tomography scanning showed a cystic frontal image with contralateral ventricle displacement. surgical intervention was performed. purulent material culture was positive for citrobacter koseri (diversus). cerebrospinal fluid culture was negative. the infant was treated with antibiotics for 6 weeks with a good outcome. she presented postsurgical hydrocep ... | 2009 | 20049403 |
| the natural cytotoxicity receptor nkp46 is dispensable for il-22-mediated innate intestinal immune defense against citrobacter rodentium. | natural cytotoxicity receptors (including nkp30, nkp44, and nkp46 in humans and nkp46 in mice) are type i transmembrane proteins that signal nk cell activation via itam-containing adapter proteins in response to stress- and pathogen-induced ligands. although murine nkp46 expression (encoded by ncr1) was thought to be predominantly restricted to nk cells, the identification of distinct intestinal nkp46(+) cell subsets that express the transcription factor rorc and produce il-22 suggests a broader ... | 2009 | 19846871 |
| bicarbonate-mediated stimulation of rega, the global virulence regulator from citrobacter rodentium. | the global virulence regulatory protein rega, an arac-like regulator, controls the expression of more than 60 genes in the mouse enteric pathogen citrobacter rodentium. in the presence of bicarbonate, rega activates the transcription of a number of virulence determinants and inhibits the expression of a series of housekeeping genes. to elucidate the molecular mechanism by which bicarbonate stimulates rega activity, we carried out biophysical and mutational analyses. our data indicate that rega e ... | 2009 | 19853617 |
| brain abscesses due to citrobacter koseri in a pair of twins. | 2009 | 19859021 | |
| comparison of antimicrobial efficacy of a fixed dose combination of ceftazidime + sulbactam with ceftazidime and sulbactam alone against five bacteria. | the efficacy of the agents was evaluated on the basis of minimum inhibitory concentration (mic) and time-kill curve analysis in pseudomonas aeruginosa, staphylococcus aureus, acinetobacter baumannii, mycobacterium smegmatis, and citrobacter braakii; mic was found to be 0.5, 4.0, 0.015625, 0.0078125, and 0.0625 mg/l in fdc of ceftazidime + sulbactam, respectively, which is lower than ceftazidime and sulbactam individually. time-kill curve analysis demonstrated maximum killing of bacteria after 4 ... | 2009 | 19937210 |
| purification, characterization, and gene cloning of glucose-1-phosphatase from citrobacter braakii. | citrobacter braakii produced an intracellular acid glucose phosphatase (agpc) which was purified 986 fold to homogeneity with the specific activity of 286 units/mg. agpc hydrolyzed a wide variety of phosphorylated compounds with high activity for glucose-1-phosphate and glucose-6-phosphate. the optimum ph and temperature for the enzyme activity was ph 5.0 and 45 degrees c, respectively. the km value for glucose-1-phosphate was 5.12 mm with a vmax 27.8 u mg(-1). its molecular weight was 46 kda by ... | 2009 | 19940380 |
| endogenous endophthalmitis caused by citrobacter koseri. | endogenous endophthalmitis occurs when organisms are hematogenously disseminated in to the eye from a distant focus of infection. the most common isolated organisms that cause endogenous endophthalmitis are klebsiella pneumoniae and escherichia coli. previous reports on endophthalmitis caused by citrobacter species are limited. we present the first case of endogenous endophthalmitis caused by citrobacter koseri bacteremia and renal abscesses. | 2009 | 19834321 |
| induction of intestinal th17 cells by segmented filamentous bacteria. | the gastrointestinal tract of mammals is inhabited by hundreds of distinct species of commensal microorganisms that exist in a mutualistic relationship with the host. how commensal microbiota influence the host immune system is poorly understood. we show here that colonization of the small intestine of mice with a single commensal microbe, segmented filamentous bacterium (sfb), is sufficient to induce the appearance of cd4(+) t helper cells that produce il-17 and il-22 (th17 cells) in the lamina ... | 2009 | 19836068 |
| microglial activation by citrobacter koseri is mediated by tlr4- and myd88-dependent pathways. | citrobacter koseri is a gram-negative bacterium that can cause a highly aggressive form of neonatal meningitis, which often progresses to establish multifocal brain abscesses. despite its tropism for the brain parenchyma, microglial responses to c. koseri have not yet been examined. microglia use tlrs to recognize invading pathogens and elicit proinflammatory mediator expression important for infection containment. in this study, we investigated the importance of the lps receptor tlr4 and myd88, ... | 2009 | 19812209 |
| infective endocarditis due to citrobacter koseri in an immunocompetent adult. | citrobacter koseri (formerly citrobacter diversus) is a motile gram-negative bacillus usually arising from urinary and gastrointestinal tracts. c. koseri rarely causes infection in immunocompetent patients and, thus far, has been considered an opportunistic pathogen. we report on a 30-year-old man, with no medical past, hospitalized for infective aortic endocarditis due to c. koseri. four weeks of antibiotherapy led to a full recovery for this patient. however, this case is unusual, as previous ... | 2009 | 19812281 |
| conjugal transformation and transposon and chemical mutagenesis of gram-negative selenate-respiring citrobacter sp. strain jsa. | conjugal mating between selenate-reducing citrobacter sp. strain jsa and escherichia coli s17-1 bearing psup2021 allowed transposon mutagenesis and chromosomal transformation. kanamycin-resistant transconjugants were obtained successfully by this method from a freshwater selenate-respiring citrobacter sp. strain jsa. the maximum frequency of kanamycin-resistant tn5 transconjugants was 3.6 x 10(-6) per recipient of this strain. of these transconjugants, eight strains of selenate reduction-deficie ... | 2009 | 19365687 |