Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| minor nucleotide substitutions in the omp31 gene of brucella ovis result in antigenic differences in the major outer membrane protein that it encodes compared to those of the other brucella species. | the gene coding for the major outer membrane protein omp31 was sequenced in five brucella species and their biovars. although the omp31 genes appeared to be highly conserved in the genus brucella, nine nucleotide substitutions were detected in the gene of brucella ovis compared to that of brucella melitensis. as shown by differential binding properties of monoclonal antibodies (mabs) to the two brucella species, these nucleotide substitutions result in different antigenic properties of omp31. th ... | 2001 | 11598077 |
| congeners of smap29 kill ovine pathogens and induce ultrastructural damage in bacterial cells. | smap29, an ovine cathelicidin, was systematically altered to create a family of 23 related peptides for mic and minimum bactericidal concentration determinations. smap28, smap29, and a derivative of smap29 called ovispirin were all antimicrobial. however, many congeners of smap29 and ovispirin were not as active as the parent molecules. with immunoelectron microscopy, smap29 was seen on membranes and within the cytoplasm of pseudomonas aeruginosa pao1. | 2001 | 11600395 |
| characterization of specific immune responses of mice inoculated with recombinant vaccinia virus expressing an 18-kilodalton outer membrane protein of brucella abortus. | using the shuttle vector pmco2 and the vaccinia virus wild-type wr strain, we constructed a recombinant virus expressing an 18-kda outer membrane protein of brucella abortus. balb/c mice inoculated with this virus produced 18-kda protein-specific antibodies, mostly of immunoglobulin g2a isotype, and in vitro stimulation of splenocytes from these mice with purified maltose binding protein-18-kda protein fusion resulted in lymphocyte proliferation and gamma interferon production. however, these mi ... | 2000 | 10618289 |
| identification of brucella suis genes affecting intracellular survival in an in vitro human macrophage infection model by signature-tagged transposon mutagenesis. | bacteria of the genus brucella are facultative intracellular pathogens which have developed the capacity to survive and multiply in professional and nonprofessional phagocytes. the genetic basis of this aspect of brucella virulence is still poorly understood. to identify new virulence factors, we have adapted signature-tagged transposon mutagenesis, which has been used essentially in animal models, to an in vitro human macrophage infection model. a library of 1,152 brucella suis 1330 tagged mini ... | 2000 | 10678941 |
| molecular characterization of brucella strains isolated from marine mammals. | recently, gram-negative bacteria isolated from a variety of marine mammals have been identified as brucella species by conventional phenotypic analysis. this study found the 16s rrna gene from one representative isolate was identical to the homologous sequences of brucella abortus, b. melitensis, b. canis, and b. suis. is711-based dna fingerprinting of 23 isolates from marine mammals showed all the isolates differed from the classical brucella species. in general, fingerprint patterns grouped by ... | 2000 | 10699036 |
| characterization of heat, oxidative, and acid stress responses in brucella melitensis. | brucella melitensis is a facultative intracellular pathogen which is able to survive and replicate within phagocytic cells. therefore, it has to adapt to a range of different hostile environments. in order to understand the mechanisms of intracellular survival employed by virulent b. melitensis 16m, an initial approach consisting of analysis of the differences in patterns of protein synthesis in response to heat, oxidative, and acid ph stresses by two-dimensional (2-d) polyacrylamide gel electro ... | 2000 | 10768994 |
| brucella abortus and its closest phylogenetic relative, ochrobactrum spp., differ in outer membrane permeability and cationic peptide resistance. | the outer membrane (om) of the intracellular parasite brucella abortus is permeable to hydrophobic probes and resistant to destabilization by polycationic peptides and edta. the significance of these unusual properties was investigated in a comparative study with the opportunistic pathogens of the genus ochrobactrum, the closest known brucella relative. ochrobactrum spp. oms were impermeable to hydrophobic probes and sensitive to polymyxin b but resistant to edta. these properties were traced to ... | 2000 | 10816465 |
| overexpression of protective antigen as a novel approach to enhance vaccine efficacy of brucella abortus strain rb51. | brucella abortus strain rb51 is an attenuated rough strain that is currently being used as the official live vaccine for bovine brucellosis in the united states and several other countries. we reasoned that overexpression of a protective antigen(s) of b. abortus in strain rb51 should enhance its vaccine efficacy. to test this hypothesis, we overexpressed cu/zn superoxide dismutase (sod) protein of b. abortus in strain rb51. this was accomplished by transforming strain rb51 with a broad-host-rang ... | 2000 | 10816475 |
| complementation of brucella abortus rb51 with a functional wboa gene results in o-antigen synthesis and enhanced vaccine efficacy but no change in rough phenotype and attenuation. | brucella abortus rb51 is a stable rough, attenuated mutant vaccine strain derived from the virulent strain 2308. recently, we demonstrated that the wboa gene in rb51 is disrupted by an is711 element (r. vemulapalli, j. r. mcquiston, g. g. schurig, n. srirauganathan, s. m. halling, and s. m. boyle, clin. diagn. lab. immunol. 6:760-764, 1999). disruption of the wboa gene in smooth, virulent b. abortus, brucella melitensis, and brucella suis results in rough, attenuated mutants which fail to produc ... | 2000 | 10858205 |
| identification of protective outer membrane antigens of brucella ovis by passive immunization of mice with monoclonal antibodies. | outer membrane proteins (omps) and rough lipopolysaccharide (r-lps), the main surface antigens of brucella ovis, display surface-exposed epitopes. mixtures of monoclonal antibodies (mabs) to both antigens were previously shown to protect mice against a b. ovis challenge. to further identify the antigens involved, seven mabs against brucella omps (omp10, omp16, omp19, omp25, omp31, omp2b and omp1) and three to r-lps were tested for protection either individually or in combinations. significant re ... | 2000 | 10865193 |
| a multiplex approach to molecular detection of brucella abortus and/or mycobacterium bovis infection in cattle. | a multiplex amplification and detection platform for the diagnosis of mycobacterium bovis and brucella abortus infection simultaneously in bovine milk and nasal secretions was developed. this system (designated the bovine pathogen detection assay [bpda]-pcr) consists of duplex amplification of species-specific targets (a region of the bcsp31k gene of b. abortus and a repeat-sequence region in the hsp65 gene of m. bovis, respectively). this is followed by a solid-phase probe capture hybridization ... | 2000 | 10878051 |
| fluorescent whole-cell hybridization with 16s rrna-targeted oligonucleotide probes to identify brucella spp. by flow cytometry. | a whole-cell hybridization assay with fluorescent oligonucleotide probes derived from the 16s rrna sequence of brucella abortus in combination with flow cytometry has been developed. with the three fluorescent probes selected, a positive signal was observed with all the representative strains of the species and biovars of brucella and with a total of nine different brucella clinical isolates. using the b9 probe in the hybridization assay, it was possible to discriminate between brucella suis bio ... | 2000 | 10878084 |
| validation of the abbreviated brucella amos pcr as a rapid screening method for differentiation of brucella abortus field strain isolates and the vaccine strains, 19 and rb51. | the brucella amos pcr assay was previously developed to identify and differentiate specific brucella species. in this study, an abbreviated brucella amos pcr test was evaluated to determine its accuracy in differentiating brucella abortus into three categories: field strains, vaccine strain 19 (s19), and vaccine strain rb51/parent strain 2308 (s2308). two hundred thirty-one isolates were identified and tested by the conventional biochemical tests and brucella amos pcr. this included 120 isolates ... | 2000 | 10921983 |
| evaluation of tests employed in serological diagnosis of brucellosis caused by brucella ovis. | a survey was carried out to verify the sensitivity and specificity of various tests (complement fixation test--cf; agar gel immunodiffusion--agid; indirect enzyme linked immunosorbent assay--elisa; immunoblotting--ib) employed in the serological diagnosis of brucellosis caused by brucella ovis. the tests were executed on 44 blood serum samples of rams coming from b. ovis-free flocks, 75 of b. ovis experimentally infected rams and 1139 from rams living in flocks where b. ovis had been previously ... | 2000 | 10939043 |
| a homologue of an operon required for dna transfer in agrobacterium is required in brucella abortus for virulence and intracellular multiplication. | as part of a brucella abortus 2308 genome project carried out in our laboratory, we identified, cloned, and sequenced a genomic dna fragment containing a locus (virb) highly homologous to bacterial type iv secretion systems. the b. abortus virb locus is a collinear arrangement of 13 open reading frames (orfs). between virb1 and virb2 and downstream of orf12, two degenerated, palindromic repeat sequences characteristic of brucella intergenic regions were found. gene reporter studies demonstrated ... | 2000 | 10940027 |
| an immunoblotting technique for the serodiagnosis of brucellosis by brucella ovis. | an immunoblotting (ib) technique was developed for the serodiagnosis of brucellosis caused by brucella ovis. immunoblotting was performed, using a b. ovis hs (hot saline extract) antigen, on 44 blood serum samples which came from rams belonging to known brucella-free flocks, 114 samples originating from ten experimentally b. ovis infected rams and 100 from rams of naturally b. ovis infected flocks. no bands were noted on any of the 44 serum samples which originated from known negative flocks. se ... | 2000 | 10946406 |
| an is711 element downstream of the bp26 gene is a specific marker of brucella spp. isolated from marine mammals. | dna polymorphism of the bp26 gene, coding for a diagnostic protein antigen for brucellosis, was assessed by pcr and restriction fragment length polymorphism analysis using primers to amplify the bp26 gene with its flanking regions. surprisingly, whereas pcr performed on dna of the reference strains of the six recognized brucella species produced a product of the expected size (1,029 bp), pcr performed on dna of three representative strains from marine mammals (from a seal, a dolphin, and a porpo ... | 2000 | 10973465 |
| identification and characterization of the brucella abortus phosphoglucomutase gene: role of lipopolysaccharide in virulence and intracellular multiplication. | smooth lipopolysaccharide (lps) of brucella abortus has been reported to be an important virulence factor, although its precise role in pathogenesis is not yet clear. while the protective properties of lps against complement are well accepted, there is still some controversy about the capacity of rough mutants to replicate intracellularly. the b. abortus phosphoglucomutase gene (pgm) was cloned, sequenced, and disrupted. the gene has a high index of identity to agrobacterium tumefaciens pgm but ... | 2000 | 10992476 |
| transmission of brucella ovis from rams to red deer stags. | to determine whether b. ovis will transmit from infected rams to non-infected red deer stags (cervus elaphus) grazing together in the same paddock. | 2000 | 16032119 |
| attempted transmission of brucella ovis between red deer stags by successive grazing or adjacent-paddock grazing. | to determine whether brucella ovis can be transmitted from stag to stag by successive grazing of infected and noninfected stags in the same paddock, or by grazing infected and non-infected stags in adjacent paddocks. | 2000 | 16032138 |
| performance of an enzyme-linked immunosorbent assay for the diagnosis of brucella ovis infection in rams. | to describe the performance characteristics (sensitivity and specificity) of an enzyme-linked immunosorbent assay (elisa) for the diagnosis of brucella ovis infection in rams. | 1999 | 16032074 |
| cross-reactivity between the rheumatoid arthritis-associated motif eqkraa and structurally related sequences found in proteus mirabilis. | cross-reactivity or molecular mimicry may be one of the underlying mechanisms involved in the etiopathogenesis of rheumatoid arthritis (ra). antiserum against the ra susceptibility sequence eqkraa was shown to bind to a similar peptide esrral present in the hemolysin of the gram-negative bacterium proteus mirabilis, and an anti-esrral serum reacted with eqkraa. there was no reactivity with either anti-eqkraa or anti-esrral to a peptide containing the ederaa sequence which is present in hla-drb1* ... | 1999 | 10338479 |
| discontinuous occurrence of the hsp70 (dnak) gene among archaea and sequence features of hsp70 suggest a novel outlook on phylogenies inferred from this protein. | occurrence of the hsp70 (dnak) gene was investigated in various members of the domain archaea comprising both euryarchaeotes and crenarchaeotes and in the hyperthermophilic bacteria aquifex pyrophilus and thermotoga maritima representing the deepest offshoots in phylogenetic trees of bacterial 16s rrna sequences. the gene was not detected in 8 of 10 archaea examined but was found in a. pyrophilus and t. maritima, from which it was cloned and sequenced. comparative analyses of the hsp70 amino aci ... | 1999 | 9882656 |
| vaccination with live escherichia coli expressing brucella abortus cu/zn superoxide dismutase protects mice against virulent b. abortus. | vaccination of mice with escherichia coli expressing brucella cu/zn superoxide dismutase (sod) [e. coli(pbssod)] induced a significant level of protection against virulent brucella abortus challenge, although this level was not as high as the one reached with b. abortus vaccine strain rb51. in addition, vaccination with e. coli(pbssod) induced antibodies to cu/zn sod and a strong proliferative response of splenocytes when stimulated in vitro with a thioredoxin-cu/zn sod fusion protein. | 1999 | 9916121 |
| cloning and expression of the dnak gene of campylobacter jejuni and antigenicity of heat shock protein 70. | campylobacter jejuni is a leading cause of infectious diarrhea throughout the world. in addition, there is growing evidence that guillain-barré syndrome, an inflammatory demyelinating disease of the peripheral nervous system, is frequently preceded by c. jejuni infection. in the present study, the hrca-grpe-dnak gene cluster of c. jejuni was cloned and sequenced. the dnak gene consists of an open reading frame of 1,869 bp and encodes a protein with a high degree of homology to other bacterial 70 ... | 1999 | 10024560 |
| genomic fingerprinting and development of a dendrogram for brucella spp. isolated from seals, porpoises, and dolphins. | genomic dna from reference strains and biovars of the genus brucella was analyzed using pulsed-field gel electrophoresis (pfge). fingerprints were compared to estimate genetic relatedness among the strains and to obtain information on evolutionary relationships. electrophoresis of dna digested with the restriction endonuclease xbai produced fragment profiles for the reference type strains that distinguished these strains to the level of species. included in this study were strains isolated from ... | 1999 | 10098687 |
| effect of early antibiotic treatment on the antibody response to cytoplasmic proteins of brucella melitensis in mice. | to test whether antibiotic therapy hampers the antibody response to brucella antigens, 30 balb/c mice were infected with brucella melitensis h38 and randomized for treatment with doxycycline administered intraperitoneally for 42 days starting at 7 or 28 days postinfection (p.i.) (groups dox7 and dox28, respectively) or for no treatment (control group). antibodies to smooth lipopolysaccharide (lps) reached peak levels (mean optical density [od] = 2.618) between days 56 and 70 p.i. in the control ... | 1999 | 10225853 |
| molecular characterization of a brucella species large dna fragment deleted in brucella abortus strains: evidence for a locus involved in the synthesis of a polysaccharide. | a brucella melitensis 16m dna fragment of 17,119 bp, which contains a large region deleted in b. abortus strains and dna flanking one side of the deletion, has been characterized. in addition to the previously identified omp31 gene, 14 hypothetical genes have been identified in the b. melitensis fragment, most of them showing homology to genes involved in the synthesis of a polysaccharide. considering that 10 of the 15 genes are missing in b. abortus and that all the polysaccharides described in ... | 1999 | 10338472 |
| experimental brucella ovis infection in pregnant ewes. | forty yearling brucella-free ewes were inoculated with brucella ovis by the conjunctival route in mid or late first pregnancy. only a few ewes excreted b ovis during pregnancy and gave birth to stillborn lambs, but most of them excreted the organism at lambing or during lactation. one of the 11 lambs which were born alive but died before they were weaned was found to be infected postmortem. in contrast, none of the 46 surviving lambs which were reared in isolation until adulthood, was found to b ... | 1999 | 10371013 |
| brucella outer membrane lipoproteins share antigenic determinants with bacteria of the family rhizobiaceae. | brucellae have been reported to be phylogenetically related to bacteria of the family rhizobiaceae. in the present study, we used a panel of monoclonal antibodies (mabs) to brucella outer membrane proteins (omps) to determine the presence of common omp epitopes in some representative bacteria of this family, i.e., ochrobactrum anthropi, phyllobacterium rubiacearum, rhizobium leguminosarum, and agrobacterium tumefaciens, and also in bacteria reported to serologically cross-react with brucella, i. ... | 1999 | 10391877 |
| genetic characterization of a tn5-disrupted glycosyltransferase gene homolog in brucella abortus and its effect on lipopolysaccharide composition and virulence. | we constructed a rough mutant of brucella abortus 2308 by transposon (tn5) mutagenesis. neither whole cells nor extracted lipopolysaccharide (lps) from this mutant, designated ra1, reacted with a brucella o-side-chain-specific monoclonal antibody (mab), bru-38, indicating the absence of o-side-chain synthesis. compositional analyses of lps from strain ra1 showed reduced levels of quinovosamine and mannose relative to the levels in the parental, wild-type strain, 2308. we isolated dna flanking th ... | 1999 | 10417145 |
| epididymitis by brucella ovis: experimental infection in virgin ram lambs. | ten sexually immature rams were experimentally infected with brucella ovis, to verify the antibody kinetics and its localization in urinary and genital tracts. all animals became positive to the complement fixation test from the 2nd post infection (p.i.) week and reached the maximum titre (1:256) on the 4th p.i. week. bacteriemia was demonstrated on 3rd, 4th and 5th p.i. weeks. two animals, respectively slaughtered 11 and 13 weeks after the infection, showed macroscopic and microscopic genital l ... | 1999 | 10423741 |
| outer membrane proteins omp10, omp16, and omp19 of brucella spp. are lipoproteins. | the deduced sequences of the omp10, omp16, and omp19 outer membrane proteins of brucella spp. contain a potential bacterial lipoprotein processing sequence. after extraction with triton x-114, these three proteins partitioned into the detergent phase. processing of the three proteins is inhibited by globomycin, a specific inhibitor of lipoprotein signal peptidase. the three proteins were radioimmunoprecipitated from [(3)h]palmitic acid-labeled brucella abortus lysates with monoclonal antibodies. ... | 1999 | 10456959 |
| identification of an is711 element interrupting the wboa gene of brucella abortus vaccine strain rb51 and a pcr assay to distinguish strain rb51 from other brucella species and strains. | brucella abortus vaccine strain rb51 is a natural stable attenuated rough mutant derived from the virulent strain 2308. the genetic mutations that are responsible for the roughness and the attenuation of strain rb51 have not been identified until now. also, except for an assay based on pulsed-field gel electrophoresis, no other simple method to differentiate strain rb51 from its parent strain 2308 is available. in the present study, we demonstrate that the wboa gene encoding a glycosyltransferas ... | 1999 | 10473532 |
| improvement of the immune response to foot and mouth disease virus vaccine in calves by using avridine as adjuvant. | the epidemiological analysis of the cattle population during the eradication plan of foot and mouth disease (fmd) in argentina clearly indicated a higher incidence of the disease in animals within their first year of age. it is important to improve the efficacy of the vaccination in those animals. in a previous report, we have shown the effect of an immunomodulator, avridine (avr), in the enhancement of the immune response elicited by fmd virus (fmdv) vaccines in experimental hosts [berinstein, ... | 1999 | 10490231 |
| [isolation of brucella ovis from semen of sheep seropositive by elisa and with clinically normal genitals]. | 1999 | 10509410 | |
| protection of mice against brucellosis by vaccination with brucella melitensis wr201(16mdeltapurek). | human brucellosis can be acquired from infected animal tissues by ingestion, inhalation, or contamination of the conjunctiva or traumatized skin by infected animal products. a vaccine to protect humans from occupational exposure or from zoonotic infection in areas where the disease is endemic would reduce an important cause of morbidity worldwide. vaccines currently used in animals are unsuitable for human use. we tested a live, attenuated, purine-auxotrophic mutant strain of brucella melitensis ... | 1999 | 10531243 |
| the outer membrane of brucella ovis shows increased permeability to hydrophobic probes and is more susceptible to cationic peptides than are the outer membranes of mutant rough brucella abortus strains. | the permeability of the outer membrane (om) to hydrophobic probes and its susceptibility to bactericidal cationic peptides were investigated for natural rough brucella ovis and for mutant rough brucella abortus strains. the om of b. ovis displayed an abrupt and faster kinetic profile than rough b. abortus during the uptake of the hydrophobic probe n-phenyl-naphthylamine. b. ovis was more sensitive than rough b. abortus to the action of cationic peptides. bactenecins 5 and 7 induced morphological ... | 1999 | 10531286 |
| complement fixation test to assess humoral immunity in cattle and sheep vaccinated with brucella abortus rb51. | the live attenuated brucella abortus strain rb51 is a rifampin-resistant, lipopolysaccharide (lps) o-chain-deficient mutant of virulent b. abortus 2308. the reduced o-chain content in rb51 prevents this bacterium from inducing antibodies detectable by the conventional serologic tests for bovine brucellosis diagnosis that mainly identify antibodies to lps. the absence of available serologic tests for rb51 also complicates the diagnosis of possible rb51 infections in humans exposed to this strain. ... | 1999 | 10548564 |
| simultaneous identification of antibodies to brucella abortus and staphylococcus aureus in milk samples by flow cytometry. | two flow cytometric assays are described herein. the single cytometric test (sct) detects antibodies to either brucella abortus or staphylococcus aureus in the serum or milk of a cow or water buffalo. the double cytometric test (dct) detects both anti-b. abortus and anti-s. aureus antibodies concurrently. in the sct, the sample to be tested is incubated in succession with the antigen (either b. abortus or s. aureus) and the proper secondary antiserum (fluorescein isothiocyanate-labelled rabbit a ... | 1998 | 9508316 |
| characterization of an immuno-dominant antigen in brucella ovis and evaluation of its use in an enzyme-linked immunosorbent assay. | a panel of 45 brucella ovis serologically positive sera were tested in immunoblots against b. ovis outer membrane proteins omp31 and omp25, purified by preparative sds-gel electrophoresis. forty-three sera reacted with omp31, while only 11 reacted with omp25, suggesting that omp31 is identical to the previously reported immuno-dominant 29-kda protein. attempts to purify omp31 on a larger scale by using procedures such as ion exchange-, reversed phase-, affinity- and gel filtration chromatography ... | 1998 | 9549861 |
| the regulated outer membrane protein omp21 from comamonas acidovorans is identified as a member of a new family of eight-stranded beta-sheet proteins by its sequence and properties. | omp21, a minor outer membrane protein of the soil bacterium comamonas acidovorans, was purified from a spontaneous mutant lacking a surface layer and long-chain lipopolysaccharide. omp21 synthesis is enhanced by oxygen depletion, and the protein has a variable electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gel electrophoresis due to its heat-modifiable behavior. the structural gene omp21 encodes a precursor of 204 amino acids with a putative signal peptide of 21 amino acids. m ... | 1998 | 9683466 |
| identification of brucella by ribosomal-spacer-region pcr and differentiation of brucella canis from other brucella spp. pathogenic for humans by carbohydrate profiles. | molecular and chemical characteristics often provide complementary information in the differentiation of closely related organisms. the genus brucella consists of a highly conserved group of organisms. identification of the four species pathogenic in humans (brucella melitensis, brucella abortus, brucella suis, and brucella canis) is problematic for many clinical laboratories that depend primarily on serology and phenotypic characteristics to differentiate species. pcr amplification of the 16s-2 ... | 1998 | 9774568 |
| murine immune responses to neisseria meningitidis group c capsular polysaccharide and a thymus-dependent toxoid conjugate vaccine. | the polysaccharide (ps) capsules of many pathogenic bacteria are poor immunogens in infants and young children as a result of the delayed response to ps antigens during ontogeny. the development of polysaccharide-protein conjugate vaccines for haemophilus influenzae type b, which have proven to be efficacious in this age group, has led to active development by a number of investigators of conjugate vaccines for other diseases. we describe here the response of several mouse strains to the capsula ... | 1998 | 9784556 |
| effect of p39 gene deletion in live brucella vaccine strains on residual virulence and protective activity in mice. | the 39-kilodalton protein (p39) has previously been shown to be an immunodominant protein in brucella infections. p39 gene deletion mutants of vaccine strains brucella abortus s19 and brucella melitensis rev.1 were constructed by gene replacement. this deletion did not significantly modify the residual virulence of both vaccine strains in cd-1 mice. cd-1 mice vaccinated with the parent or mutant strains were protected against a virulent challenge. mutant vaccine strains devoid of p39 could provi ... | 1998 | 9784574 |
| evaluation of lipopolysaccharides and polysaccharides of different epitopic structures in the indirect enzyme-linked immunosorbent assay for diagnosis of brucellosis in small ruminants and cattle. | brucella abortus and brucella melitensis have surface lipopolysaccharides and polysaccharides carrying b. melitensis-type (m) and b. abortus-type (a) epitopes as well as common (c) epitopes present in all smooth brucella biotypes. crude lipopolysaccharides, hydrolytic o polysaccharides, and native hapten polysaccharides of mc or ac specificity were evaluated in indirect enzyme-linked immunosorbent assays with polyclonal, monoclonal, or protein g conjugates by using sera from cattle, sheep, and g ... | 1998 | 9801329 |
| o-polysaccharide epitopic heterogeneity at the surface of brucella spp. studied by enzyme-linked immunosorbent assay and flow cytometry. | smooth brucella strains are classified into three serotypes, i.e., a+m-, a-m+, and a+m+, according to slide agglutination with a and m monospecific polyclonal sera. the epitopes involved have been located on the o-polysaccharide (o-ps) moiety of the smooth lipopolysaccharide (s-lps), which represents the most exposed antigenic structure on the surface of brucella spp. by use of monoclonal antibodies (mabs) a number of epitope specificities on the o-ps have been reported: a, m, and epitopes share ... | 1998 | 9801349 |
| comparison of polyclonal, monoclonal and protein g peroxidase conjugates in an enzyme-linked immunosorbent assay for the diagnosis of brucella ovis in sheep. | the sensitivities of three commercial peroxidase conjugates (polyclonal anti-sheep igg, recombinant protein g and a monoclonal anti-ruminant igg1) in an enzyme-linked immunosorbent assay for brucella ovis were evaluated. the monoclonal and protein g conjugates reduced, but did not totally remove, the characteristic background reactivity observed with the sera from brucella-free sheep. the protein g conjugate provided the best sensitivity, similar to that obtained with the classical gel diffusion ... | 1998 | 9802197 |
| identification of a 71-kilodalton surface-associated hsp70 homologue in coxiella burnetii. | a coxiella burnetii hsp70 homologue was identified by using an acid activation in vitro system in which protein synthesis has been followed by [35s]methionine labeling, autoradiography, and immunoblotting. the protein was one of those predominantly labeled, and the immunoblots revealed that it was recognized by anti-dnak antibodies. the corresponding gene was isolated, and its nucleotide sequence was determined and analyzed. a single open reading frame (orf) with a size of 1,968 bp was identifie ... | 1998 | 9826369 |
| epidemiological observations in a corriedale flock affected by brucella ovis. | brucellosis in sheep, caused by brucella ovis, is primarily a chronic infectious disease of rams with epididymitis as its most characteristic lesion. six hundred rams from an infected farm were clinically and serologically examined once a year, over a 3-year period. an increase from 2.1% to 6.3% in the prevalence of animals serologically positive to b. ovis occurred over the 3 years. however, the prevalence of rams with lesions in the reproductive tract declined from 14.2% to 6.5% in the third y ... | 1998 | 9868758 |
| stability of antigen and agarose used in a double immunodiffusion serologic test for brucella ovis. | 1998 | 9580326 | |
| induction of systemic and mucosal immune responses to human immunodeficiency virus type 1 by a dna vaccine formulated with qs-21 saponin adjuvant via intramuscular and intranasal routes. | induction of mucosal and cell-mediated immunity is critical for development of an effective vaccine against human immunodeficiency virus (hiv). we compared intramuscular and intranasal immunizations with a dna vaccine encoding env of hiv-1 and evaluated the qs-21 saponin adjuvant for augmentation of the systemic and mucosal immune responses to hiv-1 in a murine model. vaccination via the two routes elicited comparable systemic immune responses, and qs-21 consistently enhanced antigen-specific se ... | 1998 | 9573261 |
| transposon-derived brucella abortus rough mutants are attenuated and exhibit reduced intracellular survival. | the o antigen of brucella abortus has been described as a major virulence determinant based on the attenuated survival of fortuitously isolated rough variants. however, the lack of genetic definition of these mutants and the virulence of naturally occurring rough species, brucella ovis and brucella canis, has confused interpretation. to better characterize the role of o antigen in virulence and survival, transposon mutagenesis was used to generate b. abortus rough mutants defective in o-antigen ... | 1998 | 9488389 |
| evaluation of electrophoretic immunoblotting for brucella ovis infection in deer using ram and deer serum. | recently the first case of natural infection of deer with brucella ovis was discovered. the aim of this study was to develop and evaluate an electrophoretic immunoblotting method for testing deer serum for specific b. ovis antibodies. | 1998 | 16032007 |
| insertion sequences. | insertion sequences (iss) constitute an important component of most bacterial genomes. over 500 individual iss have been described in the literature to date, and many more are being discovered in the ongoing prokaryotic and eukaryotic genome-sequencing projects. the last 10 years have also seen some striking advances in our understanding of the transposition process itself. not least of these has been the development of various in vitro transposition systems for both prokaryotic and eukaryotic e ... | 1998 | 9729608 |
| an improved immunoblotting technique for the serodiagnosis of brucella ovis infections. | 1997 | 22133200 | |
| an improved immunoblotting technique for the serodiagnosis of brucella ovis infections. | two antigen preparations, the routinely used brucella ovis sodium dodecylsulfate-mercapto ethanol extract and a b. ovis triton x-114-derived detergent-rich phase, were compared under standard conditions for their use in electrophoretic immunoblotting for confirmafory, serological testing for b. ovis infections, by using 88 sera from ram flocks with a history of freedom from b. ovis infections, 80 sera from chronically infected rams, which were shedding b. ovis in their semen at the time of sampl ... | 1997 | 16031955 |
| development and validation of an indirect enzyme immunoassay for detection of ovine antibody to brucella ovis. | an indirect enzyme-linked immunosorbent assay (elisa) for the serodiagnosis of brucella ovis infection was developed. the assay uses a mouse monoclonal antibody to bovine igg1 horseradish peroxidase (hrpo) conjugate that cross-reacts with immunoglobulin from sheep and a purified antigen from brucella ovis. the elisa data were read and analyzed according to a targeting procedure. the elisa results were compared with a cold complement fixation test (cft). sera from 675 rams from three uninfected f ... | 1997 | 9100335 |
| periplasmic cyclic 1,2-beta-glucan in brucella spp. is not osmoregulated. | biosynthesis of periplasmic cyclic 1,2-beta-glucans in brucella ovis strain reo198 and b. abortus strain 519 was found to be carried out by membrane-bound enzymes that use udp-glucose (udp-glc) as donor substrate. contrary to what happens in species of the genera agrobacterium and rhizobium, the accumulation of the reaction products in brucella appeared not to be osmotically regulated. incubation of permeabilized cells with udp-[14c]glc led to the formation of soluble neutral cyclic 1,2-beta-glu ... | 1997 | 9141674 |
| antibody and delayed-type hypersensitivity responses to ochrobactrum anthropi cytosolic and outer membrane antigens in infections by smooth and rough brucella spp. | immunological cross-reactions between brucella spp. and ochrobactrum anthropi were investigated in animals and humans naturally infected by brucella spp. and in experimentally infected rams (brucella ovis infected), rabbits (brucella melitensis infected), and mice (b. melitensis and brucella abortus infected). in the animals tested, o. anthropi cytosolic proteins evoked a delayed-type hypersensitivity reaction of a frequency and intensity similar to that observed with b. melitensis brucellin. o. ... | 1997 | 9144364 |
| genetic bias in immune responses to a cassette shared by different microorganisms in patients with rheumatoid arthritis. | rheumatoid arthritis (ra) is an autoimmune disease associated with hla-drbeta1 alleles which contain the qkraa amino acid sequence in their third hypervariable region(s). the qkraa sequence is also expressed by several human pathogens. we have shown previously that an escherichia coli peptide encompassing qkraa is a target of immune responses in ra patients. here we address two questions: first, whether qkraa may function as an "immunological cassette" with similar, ra-associated, immunogenic pr ... | 1997 | 9239413 |
| identification and characterization of brucella ovis immunogenic proteins using two-dimensional electrophoresis and immunoblotting. | in a previous report, proteins from brucella melitensis were characterized by two-dimensional polyacrylamide gel electrophoresis (2-d page) and n-terminal microsequencing. in the present report, we have extended this study to the second etiologic agent in ovine brucellosis, b. ovis, responsible for ram epididymitis and infertility. the combination of 2-d gel electrophoresis and protein microsequencing facilitated the location and identification of the major proteins of b. ovis on the 2-d pattern ... | 1997 | 9298663 |
| rapid identification of rough brucella isolates by a latex coagglutination assay with the 25-kilodalton outer membrane protein and rough-lipopolysaccharide-specific monoclonal antibodies. | a latex coagglutination assay was developed to identify rough (r) isolates of brucella. latex beads were coated, via protein a, with either an anti-brucella rough-lipopolysaccharide (r-lps) monoclonal antibody (mab) or an anti-brucella 25-kda outer membrane protein (omp25) mab. slide agglutination tests were done for 68 strains of brucella spp., including type strains of all biovars as well as field isolates. latex beads coated with mab to r-lps coagglutinated only r strains, whereas latex beads ... | 1997 | 9302215 |
| dna polymorphism at the omp-31 locus of brucella spp.: evidence for a large deletion in brucella abortus, and other species-specific markers. | the omp-31 gene, encoding a major outer-membrane protein in brucella melitensis, was pcr-amplified from brucella strains representing all species and known biovars by using primers selected according to the b. melitensis 16m omp-31 published sequence. amplification of omp-31 was achieved from dna of all brucella species with the exception of brucella abortus, the only brucella species where expression of omp-31 was not detected by reactivity with an mab specific for an epitope located in omp-31. ... | 1997 | 9308175 |
| [experiences in the eradication of brucella ovis infections in sheep in tyrol]. | since 1993 a campaign to eradicate brucella ovis from sheep in tyrol, using a combination of serological test and culling, is underway. during the pilot survey from 1990 until 1992 out of 812 tested rams, 85 (10%) seroreactors were identified by elisa. in the year 1995 2263 rams were tested by elisa, 80 (3.5%) seroreactors could be identified. as the topographical distribution shows the disease is still concentrated in defined areas. the paper discusses the values of the serological tests and re ... | 1997 | 9441043 |
| the cellular response of lambs to brucella ovis before and after birth. | the immunological response of lambs to brucella ovis before and after birth was investigated. the establishment of indwelling cannulas in the efferent prescapular lymphatic ducts of foetal lambs allowed continual monitoring of the immune response of a single lymph node. foetal lambs in the last trimester of pregnancy were shown to mount a strong cell-mediated immune response to b. ovis. lymphocytes from the challenged lymph node stimulated with b. ovis in vitro usually first reacted significantl ... | 1997 | 9477485 |
| sensitivity and specificity of an elisa as a screening test for the diagnosis of brucella ovis in sheep. | a hot saline extract of brucella ovis strain reo 198 at a concentration of 5 micrograms/ml in phosphate buffer ph 7.2 was used to adsorb onto maxisorb plates and incubated at 37 degrees c during 12 h; unadsorbed excess antigen was washed off thrice with phosphate buffer containing 0.5% tween 20. as blocking agent 1% skim milk was used. the conjugate used was protein g bound to peroxidase diluted 1:100. thirty three sheep sera from bacteriologically confirmed infected animals and 39 sheep sera fr ... | 1997 | 10932721 |
| molecular characterization, occurrence, and immunogenicity in infected sheep and cattle of two minor outer membrane proteins of brucella abortus. | screening of a brucella abortus genomic library with two sets of monoclonal antibodies allowed the isolation of the genes corresponding to two minor outer membrane proteins (omp10 and omp19) found in this bacterial species. sequence analysis of the omp10 gene revealed an open reading frame capable of encoding a protein of 126 amino acids. the nucleotide sequence of the insert producing the omp19 protein contains two overlapping open reading frames, the largest of which (177 codons) was shown to ... | 1996 | 8557326 |
| nucleotide sequence and expression of the gene encoding the major 25-kilodalton outer membrane protein of brucella ovis: evidence for antigenic shift, compared with other brucella species, due to a deletion in the gene. | the nucleotide sequences encoding the major 25-kda outer membrane protein (omp) (omp25 genes) of brucella ovis 63/290, brucella melitensis 16m, brucella suis 1330, brucella canis rm6/66, and brucella neotomae 5k33 (all reference strains) were determined and compared with that of brucella abortus 544 (p. de wergifosse, p. lintermans, j. n. limet, and a. cloeckaert, j. bacteriol. 177:1911-1914, 1995). the major difference found was between the omp25 gene of b. ovis and those of the other brucella ... | 1996 | 8675306 |
| venereal shedding of ovine lentivirus in infected rams. | to assess shedding of ovine lentivirus (ovlv) in semen of infected rams with or without epididymitis. | 1996 | 8723882 |
| attempted definition by immunoblotting of the causes of reactivity in suspected false-positive sera in the brucella ovis complement fixation test. | seventy-nine suspected false-positive sera, obtained over 1 year from routine submissions for brucella ovis serological testing, were used in this study. these sera, which exhibited titres in the complement fixation test, but which because of their epidemiological history and their reactions in the enzyme-linked immunosorbent assay and gel diffusion test were suspected to be false positives, were further analysed by immunoblotting. in blots, using b. ovis antigens, rough lipopolysaccharide was i ... | 1996 | 16031926 |
| identification and characterization of immunodominant antigens during the course of infection with brucella ovis. | the seroresponse against brucella ovis of 8 intrapreputially and 6 intravenously infected rams and 9 ewes infected through mating was analyzed by electrophoretic immunoblotting. additionally, 87 sera from chronically infected rams that were shedding b. ovis in their semen, 226 sera from rams belonging to infected flocks, and 324 sera from false-positive complement fixation test (cft) reactors were examined. in all infected animals, antibody reactivity was predominantly found against 5 b. ovis co ... | 1995 | 7619904 |
| diagnosis of brucella ovis infection of rams with an elisa using protein g as conjugate. | the complement fixation and gel diffusion tests for brucella ovis ram epididymitis were compared with an indirect elisa using antigens from b ovis extracted in hot saline, rough b ovis lipopolysaccharide or a cytosolic fraction of rough b melitensis 115, and commercial anti-igg (heavy and light chain specificity) or protein g conjugates. none of the antigens and conjugates used in the elisa gave better results in terms of sensitivity and specificity than the complement fixation or the gel diffus ... | 1995 | 8540208 |
| lectin histochemical study on the reproductive tract in normal and brucella ovis-infected rams. | histochemical studies on tissue sections showed alterations of lectin-binding reactivities in the epididymis, seminal vesicle and ampulla of brucella ovis-infected rams. these modifications in the carbohydrate composition of organs participating in maturation, transport, and storage of spermatozoa, could be involved in the impaired fertility observed in this disease. | 1995 | 8593306 |
| restriction site polymorphism of the genes encoding the major 25 kda and 36 kda outer-membrane proteins of brucella. | seventy-seven brucella reference and field strains from different geographic origins and hosts representing the six recognized species and their different biovars were analysed for diversity of their genes encoding the major 25 and 36 kda outer-membrane proteins (omps) by pcr-rflp. the 25 kda omp is encoded by a single gene (omp25) whereas two closely related genes (omp2a and omp2b) encode and potentially express the 36 kda omp. analysis of pcr products of the omp25 gene digested with nine restr ... | 1995 | 7496522 |
| outer-membrane protein- and rough lipopolysaccharide-specific monoclonal antibodies protect mice against brucella ovis. | brucella ovis, a naturally virulent rough brucella species, is the aetiological agent of ram epididymitis. the identification of protective antigens is necessary to obtain a safe, specific subcellular vaccine. monoclonal antibodies (mabs) directed at both brucella outer-membrane proteins (omps) and rough lipopolysaccharide (r-lps) in a mouse protection test were used to identify potential targets for humoral immunity. mixtures of mabs directed at the 16.5-, 25-27-, 31-34- and 36-38-kda omps conf ... | 1995 | 7562998 |
| the brucella abortus rb51 vaccine does not confer protection against brucella ovis in rams. | the protective efficacy against brucella ovis of the live vaccine brucella abortus strain rb51 has been evaluated in rams using the attenuated b. melitensis strain rev 1 as a reference vaccine. sixteen brucella-free rams, 6 months of age, were vaccinated subcutaneously with 4.18 x 10(10) c.f.u. rb51. sixteen rams of the same condition and age were vaccinated subcutaneously the same day with 1.1 x 10(9) c.f.u. rev 1. fifteen similar rams were kept unvaccinated as controls. six months after vaccin ... | 1995 | 7631517 |
| isotype profiles induced in balb/c mice during foot and mouth disease (fmd) virus infection or immunization with different fmd vaccine formulations. | the igg isotype response in balb/c mice infected with fmdv or immunized with different vaccine formulations using inactivated virus particles as antigen was analyzed at various times post-inoculation. for this purpose an elisa based on polyclonal antibodies for detection and quantification of mouse igg isotypes with fmd virus (fmdv) specificity was developed. three immunomodulators, which have been shown to be very effective in inducing strong and long-lasting antibody responses (bahnemann, arch ... | 1995 | 7483770 |
| brucella ovis infection in two flocks of sheep. | brucella ovis infection could not be eradicated from two flocks of sheep despite serologically testing and culling the infected rams for four years. the hypothesis that the ewes played a major role in the maintenance of the infection in both flocks was investigated by using serological, bacteriological and pathological criteria. specific antibodies against b ovis were demonstrated in 71 ewes in the two flocks. forty-four of the seropositive ewes were slaughtered for bacteriological and pathologi ... | 1994 | 7810048 |
| bacteriologically confirmed cases of ovine epididymo-orchitis caused by brucella ovis in sub-carpathia. | during the eradication of brucella ovis infection from five large breeding ram flocks of sub-carpathia (the ukraine), the genital organs of 55 rams culled because of seropositivity in the agar-gel precipitation (agp) test and elisa were subjected to gross pathological, histopathological and bacteriological examination. the results of these examinations, as well as the properties of b. ovis strains isolated for the first time in the region are reported. thirty-three out of the 55 pairs of epididy ... | 1994 | 7810398 |
| differentiation of brucella abortus bv. 1, 2, and 4, brucella melitensis, brucella ovis, and brucella suis bv. 1 by pcr. | several pcr assays which identify the genus brucella but do not discriminate among species have been reported. we describe a pcr assay that comprises five oligonucleotide primers which can identify selected biovars of four species of brucella. individual biovars within a species are not differentiated. the assay can identify three biovars (1, 2, and 4) of b. abortus, all three biovars of b. melitensis, biovar 1 of b. suis, and all b. ovis biovars. these biovars include all of the brucella specie ... | 1994 | 7852552 |
| transmission of brucella ovis by ewes. | 1994 | 7886906 | |
| vaccination with brucella abortus rough mutant rb51 protects balb/c mice against virulent strains of brucella abortus, brucella melitensis, and brucella ovis. | vaccination of balb/c mice with live brucella abortus rb51, a stable rough mutant, produced protection against challenge with virulent strains of brucella abortus, brucella melitensis, and brucella ovis. passive-transfer experiments indicated that vaccinated mice were protected against b. abortus 2308 through cell-mediated immunity, against b. ovis pa through humoral immunity, and against b. melitensis 16m through both forms of immunity. live bacteria were required for the induction of protectiv ... | 1994 | 7927779 |
| complementation of a dnak-deficient escherichia coli strain with the dnak/dnaj operon of brucella ovis reduces the rate of initial intracellular killing within the monocytic cell line u937. | facultatively intracellular bacteria express heat shock proteins after phagocytosis by macrophages. using non-pathogenic escherichia coli strains and the human monocytic cell line u937, we showed that deletion of the dnak gene significantly increased the rate of initial intracellular killing of bacteria. trans-complementation of the deletion mutant with the dnak/dnaj operon of brucella ovis restored the pattern of intracellular elimination of the control strain expressing dnak. these differences ... | 1994 | 8076809 |
| eradication of brucella ovis from the falkland islands 1977-1993. | a campaign to eradicate brucella ovis from sheep in the falkland islands, using a combination of serological tests and culling, was initiated in 1977 and continued until 1993 when, after a total of 65,266 tests had been carried out, the organism had been eradicated from the national flock. the paper discusses the relative values of the serological tests and suggests guidelines for maintaining the flock free of the infection. | 1994 | 8085323 |
| protective immunity to brucella ovis in balb/c mice following recovery from primary infection or immunization with subcellular vaccines. | experiments were performed with balb/c mice to elucidate the roles of humoral and cell-mediated immune responses in the acquisition of protective immunity to brucella ovis and to compare infection immunity with immunity developed through vaccination with a hot saline extract (hs) of b. ovis. mice convalescing from a primary infection with b. ovis displayed a high level of resistance to reinfection, as evidenced by splenic bacterial counts decreased over 10,000-fold from control groups at 2 weeks ... | 1994 | 8300219 |
| a sensitive immunoblotting technique for the serodiagnosis of brucella ovis infections. | a simplified electrophoretic immunoblotting technique based on antigen extracted from brucella ovis cells with sodium dodecyl sulfate/mercaptoethanol was compared with the complement fixation test (cft), the enzyme-linked immunosorbent assay, and the gel diffusion test. sera from 89 chronically infected, semen culture-positive rams, 378 sera from b. ovis-infected flocks, 300 sera from accredited disease-free flocks, and 29 sera from specific-pathogen-free sheep were used. the immunoblotting tech ... | 1994 | 8068750 |
| seroprevalence of brucella ovis infection in commercial ram flocks in the tamworth area. | commercial ram flocks in the tamworth area of northern new south wales were surveyed to estimate the proportion of flocks, and rams, infected with brucella ovis. the flock prevalence (percentage of flocks containing seropositive rams) of 9.1% for merino flocks was significantly lower than that for british-breed flocks (43.8%, p=0.006) and mixed-breed flocks (46.7%, p=0.017). the mean flock prevalence over all flock types was 32.9%. these estimates were supported by data obtained from diagnostic ... | 1994 | 16031755 |
| serological and necropsy findings for rams infected with brucella ovis which were not identified by the complement fixation test. | the eradication of brucella ovis from a commercial flock of 36 romney rams was complicated by four infected rams remaining undetected despite four successive flock examinations using the complement fixation test. these four rams were subsequently tested using an enzyme-linked immunosorbent assay and a gel diffusion test and shown to be infected by semen culture. all four rams could have been identified as infected at the initial test if the enzyme-linked immunosorbent assay had been used in addi ... | 1993 | 16031700 |
| use of the double immuno gel diffusion test and the enzyme-linked immunosorbent assay to distinguish false from true reactors in the complement fixation test for brucella ovis. | a gel diffusion test with sonicated brucella ovis antigen and an enzyme-linked immunosorbent assay based on heat-extracted antigen were used to distinguish false from true reactions in a complement fixation test based on heat-extracted antigen. of 142 complement fixing reactors (occurring in supposedly brucella ovis-free, accredited flocks), the gel diffusion test correctly identified the status of 139 animals as compared to 128 with the enzyme-linked immunosorbent assay. a combination of the tw ... | 1993 | 16031707 |
| characterization of an 18-kilodalton brucella cytoplasmic protein which appears to be a serological marker of active infection of both human and bovine brucellosis. | some anticytoplasmic protein monoclonal antibodies (mabs) from mice immunized by infection with brucella ovis cells have been obtained. one of these mabs, bi24, was used to purify by immunoaffinity a protein with a pi of 5.6 and a molecular mass of 18 kda. this protein was present in all of the rough and smooth brucella species studied, but it could not be detected in yersinia enterocolitica 09. three internal peptides of this protein were partially sequenced; no homology with other bacterial pr ... | 1993 | 8370742 |
| evaluation of vaccines and of antigen therapy in a mouse model for brucella ovis. | a mouse model was developed to study brucella ovis infection. the evolution of the number of b. ovis per spleen of mice inoculated intravenously, intraperitoneally or subcutaneously was found to be independent of the sex of the mice. the number of b. ovis increased in the spleen when increasing the challenge dose up to 1.7 x 10(7). at higher doses of challenge, the response remained constant. in this model it was observed that the inoculation of brucella melitensis rev 1 vaccine or subcellular b ... | 1993 | 8427038 |
| [brucella ovis infection in rams of the "white alp" breed. case report]. | the clinical findings of seven "white alp" rams are discussed. six of the animals contracted brucella ovis and exhibited signs of illness. the seventh animal indicated no brucella ovis contact. the ejaculates of all rams showed significant changes. a spermatocele, typical for brucella ovis, could be proven, pathologically and anatomically, in only one case. brucella ovis was isolated from two samples. a brief discussion on the treatment of herds infected with brucella ovis is included. | 1993 | 8456270 |
| identification and sequence analysis of is6501, an insertion sequence in brucella spp.: relationship between genomic structure and the number of is6501 copies. | an insertion sequence (is) element of brucella ovis, named is6501, was isolated and its complete nucleotide sequence determined. is6501 is 836 bp in length and occurs 20-35 times in the b. ovis genome and 5-15 times in other brucella species. analysis of the junctions at the sites of insertion revealed a small target site duplication of four bases and inverted repeats of 17 bp with one mismatch. is6501 presents significant similarity (53.4%) with is427 identified in agrobacterium tumefaciens, su ... | 1993 | 8126444 |
| the dangers of disease transmission by artificial insemination and embryo transfer. | this review summarizes the major infectious diseases of the three major agricultural species (cattle, sheep and pigs) and horses, and presents the evidence for and against the possibility of infectious agents being transmitted between animals via the venereal route or by the use of semen or early embryos in commercial artificial insemination (ai) or embryo transfer (et). cattle feature most prominently in the widespread distribution of frozen semen, and national and international organizations h ... | 1993 | 8221041 |
| sequence and characterization of an insertion sequence, is711, from brucella ovis. | the nucleotide (nt) sequence of a previously discovered insertion in brucella ovis was determined and found to have the hallmarks of an insertion sequence (is). the element, designated is711, of 842 bp, is similar in g + c content to that of the brucella genome and is bounded by 20-bp imperfect inverted repeats (ir). the element appears to duplicate the nt ta of a consensus target site, ytar (r, purines; y, pyrimidines). when the complete nt sequence of four elements and 300 bp of the 3' ends of ... | 1993 | 8224885 |
| evaluation of whole cell and subcellular vaccines against brucella ovis in rams. | five antigen preparations from brucella ovis strain reo 198 were incorporated with the pluronic polymer l-121 and muramyl dipeptide and tested as vaccines against b. ovis infection of rams. the antigenic preparations were: (1) a fraction enriched in outer membrane proteins and rough lipopolysaccharide (hot saline extract, hs); (2) the proteins from hs substantially free of lipopolysaccharide; (3) outer membrane blebs; (4) outer membrane-peptidoglycan complexes extracted with detergent; (5) kille ... | 1993 | 8236802 |
| efficacy of brucella suis strain 2 vaccine against brucella ovis in rams. | the protective efficacy against brucella ovis of live vaccine brucella suis strain 2 (s2) and brucella melitensis strain rev 1 has been evaluated in rams. fourteen 4-month-old brucella-free aragonesa rams were vaccinated conjunctivally with 2 x 10(9) c.f.u. s2. sixteen rams of the same breed, condition and age were conjunctivally vaccinated the same day with 1.6 x 10(9) rev 1. thirteen rams were unvaccinated controls. eight months after vaccination all rams were challenged with 6 x 10(9) c.f.u. ... | 1993 | 8296481 |
| avridine and lps from brucella ovis: effect on the memory induced by foot-and-mouth disease virus vaccination in mice. | foot-and-mouth disease is one of the more economically important diseases among meat-producing biungulate species. in contrast to natural infection, current foot-and-mouth disease virus (fmdv) vaccines, prepared with inactivated virus and adjuvants, elicit short-lived protection. the immunomodulating effect on fmdv vaccines of avridine and lipopolysaccharide of brucella ovis (lps) was tested in a murine model. the duration of immunity, protection, stimulation of immunocompetent cells producing a ... | 1993 | 8296482 |
| cloning and characterization of the brucella ovis heat shock protein dnak functionally expressed in escherichia coli. | the brucella ovis dnak gene, homolog to the eukaryotic hsp70 genes, was cloned by using a drosophila melanogaster probe. comparison of b. ovis and escherichia coli sequences revealed a similar organization for the dnak and dnaj genes and putative regulatory signals. in e. coli transfected with the cloned fragment, b. ovis hsp70 was expressed at 30 and 50 degrees c apparently under the control of its own promoter. the recombinant protein and a b. ovis native protein displaying the same molecular ... | 1992 | 1459952 |