Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| interbacterial macromolecular transfer by the campylobacter fetus subsp. venerealis type iv secretion system. | we report here the first demonstration of intra- and interspecies conjugative plasmid dna transfer for campylobacter fetus. gene regions carried by a campylobacter coli plasmid were identified that are sufficient for conjugative mobilization to escherichia coli and c. fetus recipients. a broader functional range is predicted. efficient dna transfer involves the virb9 and vird4 genes of the type iv bacterial secretion system encoded by a pathogenicity island of c. fetus subsp. venerealis. complem ... | 2010 | 21115658 |
| vitamin b(6) is required for full motility and virulence in helicobacter pylori. | despite recent advances in our understanding of how helicobacter pylori causes disease, the factors that allow this pathogen to persist in the stomach have not yet been fully characterized. to identify new virulence factors in h. pylori, we generated low-infectivity variants of a mouse-colonizing h. pylori strain using the classical technique of in vitro attenuation. the resulting variants and their highly infectious progenitor bacteria were then analyzed by global gene expression profiling. the ... | 2010 | 21151756 |
| phenotypic and genotypic evidence for l-fucose utilization by campylobacter jejuni. | campylobacter jejuni remains among the leading causes of bacterial food-borne illness. the current understanding of campylobacter physiology suggests that it is asaccharolytic and is unable to catabolize exogenous carbohydrates. contrary to this paradigm, we provide evidence for l-fucose utilization by c. jejuni. the fucose phenotype, shown in chemically defined medium, is strain specific and linked to an 11-open reading frame (orf) plasticity region of the bacterial chromosome. by constructing ... | 2010 | 21193610 |
| phenotypic and genotypic evidence for l-fucose utilization by campylobacter jejuni. | campylobacter jejuni remains among the leading causes of bacterial food-borne illness. the current understanding of campylobacter physiology suggests that it is asaccharolytic and is unable to catabolize exogenous carbohydrates. contrary to this paradigm, we provide evidence for l-fucose utilization by c. jejuni. the fucose phenotype, shown in chemically defined medium, is strain specific and linked to an 11-open reading frame (orf) plasticity region of the bacterial chromosome. by constructing ... | 2010 | 21193610 |
| evaluation of pcr assays for the detection of campylobacter fetus in bovine preputial scrapings and the identification of subspecies in south african field isolates. | as a result of the high lability and slow growth of campylobacter fetus subspecies, the laboratory diagnosis of bovine genital campylobacteriosis has always been difficult. this is especially true under south african conditions, where farms are far apart, laboratories are only present in major centres and there are high ambient temperatures. in order to overcome the shortcomings associated with traditional diagnostic methods, the implementation of a molecular assay was sought. this work describe ... | 2010 | 21247013 |
| fastidious gram-negatives: identification by the vitek 2 neisseria-haemophilus card and by partial 16s rrna gene sequencing analysis. | taxonomy and identification of fastidious gram negatives are evolving and challenging. we compared identifications achieved with the vitek 2 neisseria-haemophilus (nh) card and partial 16s rrna gene sequence (526 bp stretch) analysis with identifications obtained with extensive phenotypic characterization using 100 fastidious gram negative bacteria. seventy-five strains represented 21 of the 26 taxa included in the vitek 2 nh database and 25 strains represented related species not included in th ... | 2010 | 21347215 |
| prolonged survival of campylobacter species in bovine manure compost. | the persistence of naturally occurring campylobacteria in aerobic compost constructed of manure from beef cattle that were administered chlortetracycline and sulfamethazine (as700) or from cattle not administered antibiotics (control) was examined. although there were no differences in population sizes of heterotrophic bacteria, the temperature of as700 compost was more variable and did not become as high as that of control compost. there were significant differences in water content, total carb ... | 2010 | 20023098 |
| development and evaluation of an indirect enzyme-linked immunosorbent assay for the detection of antibodies against campylobacter fetus in cattle. | campylobacteriosis is a zoonosis that occurs worldwide. infection with campylobacter fetus (c. fetus) causes infertility and abortion in sheep and cattle. the current study focuses on the sapa gene of c. fetus that encodes surface array proteins and plays an important role in the virulence of c. fetus. the sapa-n (1398bp) and sapa-c (1422bp) fragments were amplified from the c. fetussapa gene using polymerase chain reaction (pcr), and the corresponding recombinant proteins rsapa-n and rsapa-c we ... | 2010 | 20035963 |
| genetic relationships among reptilian and mammalian campylobacter fetus strains determined by multilocus sequence typing. | reptile campylobacter fetus isolates and closely related strains causing human disease were characterized by multilocus sequence typing. they shared approximately 90% nucleotide sequence identity with classical mammalian c. fetus, and there was evidence of recombination among members of these two groups. the reptile group represents a possible separate genomospecies capable of infecting humans. | 2010 | 20053851 |
| spondylodiscitis and an aortic aneurysm due to campylobacter coli. | campylobacter coli is a rare cause of bacteremia. we report here the first case of c.coli spondylodiscitis complicated by an aortic aneurysm. outcome was favourable with surgery and antibiotic therapy. | 2010 | 20132561 |
| campylobacter fetus peritonitis in a patient with an unused embedded subcutaneous peritoneal catheter. | 2010 | 20200376 | |
| modification of the campylobacter jejuni flagellin glycan by the product of the cj1295 homopolymeric-tract-containing gene. | the campylobacter jejuni flagellin protein is o-glycosylated with structural analogues of the nine-carbon sugar pseudaminic acid. the most common modifications in the c. jejuni 81-176 strain are the 5,7-di-n-acetylated derivative (pse5ac7ac) and an acetamidino-substituted version (pse5am7ac). other structures detected include o-acetylated and n-acetylglutamine-substituted derivatives (pse5am7ac8oac and pse5am7ac8glnnac, respectively). recently, a derivative of pseudaminic acid modified with a di ... | 2010 | 20338909 |
| the internal transcribed spacer region, a new tool for use in species differentiation and delineation of systematic relationships within the campylobacter genus. | the campylobacter genus consists of a number of important human and animal pathogens. although the 16s rrna gene has been used extensively for detection and identification of campylobacter species, there is currently limited information on the 23s rrna gene and the internal transcribed spacer (its) region that lies between the 16s and 23s rrna genes. we examined the potential of the 23s rrna gene and the its region to be used in species differentiation and delineation of systematic relationships ... | 2010 | 20348308 |
| evidence for a lineage of virulent bacteriophages that target campylobacter. | our understanding of the dynamics of genome stability versus gene flux within bacteriophage lineages is limited. recently, there has been a renewed interest in the use of bacteriophages as 'therapeutic' agents; a prerequisite for their use in such therapies is a thorough understanding of their genetic complement, genome stability and their ecology to avoid the dissemination or mobilisation of phage or bacterial virulence and toxin genes. campylobacter, a food-borne pathogen, is one of the organi ... | 2010 | 20353581 |
| the hp0256 gene product is involved in motility and cell envelope architecture of helicobacter pylori. | helicobacter pylori is the causative agent for gastritis, and peptic and duodenal ulcers. the bacterium displays 5-6 polar sheathed flagella that are essential for colonisation and persistence in the gastric mucosa. the biochemistry and genetics of flagellar biogenesis in h. pylori has not been fully elucidated. bioinformatics analysis suggested that the gene hp0256, annotated as hypothetical, was a flij homologue. in salmonella, flij is a chaperone escort protein for flgn and flit, two proteins ... | 2010 | 20377912 |
| recurrent gram-negative bloodstream infection: a 10-year population-based cohort study. | recurrent gram-negative bloodstream infection (bsi) has not been evaluated in a population-based setting; therefore, we performed a population-based retrospective cohort study to examine the incidence, recurrence, and mortality rates of gram-negative bsi. | 2010 | 20378069 |
| imipenem-resistant campylobacter fetus bloodstream infection. | 2010 | 20435578 | |
| two novel antibiotic resistance genes, tet(44) and ant(6)-ib, are located within a transferable pathogenicity island in campylobacter fetus subsp. fetus. | new tetracycline and streptomycin resistance genes, tet(44) and ant(6)-ib, were identified in campylobacter fetus subsp. fetus within a transferable pathogenicity island that is typically unique to campylobacter fetus subsp. venerealis. the 640-amino-acid tetracycline resistance determinant, tet 44, belongs to a class of proteins that confers resistance to tetracycline and minocycline by ribosomal protection. the 286-amino-acid streptomycin resistance determinant, ant(6)-ib, belongs to a family ... | 2010 | 20479200 |
| three-hour molecular detection of campylobacter, salmonella, yersinia, and shigella species in feces with accuracy as high as that of culture. | campylobacter jejuni and salmonella, shigella, and yersinia species (along with shiga toxin-producing escherichia coli) are the most common causes of acute bacterial diarrheal disease in the united states. current detection techniques are time-consuming, limiting usefulness for patient care. we developed and validated a panel of rapid pcr assays for the detection and identification of c. jejuni, c. coli, salmonella, and yersinia species and shigella and enteroinvasive e. coli in stool samples. a ... | 2010 | 20519461 |
| the rdgc protein employs a novel mechanism involving a finger domain to bind to circular dna. | the dna-binding protein rdgc has been identified as an inhibitor of reca-mediated homologous recombination in escherichia coli. in neisseria species, rdgc also has a role in virulence-associated antigenic variation. we have previously solved the crystal structure of the e. coli rdgc protein and shown it to form a toroidal dimer. in this study, we have conducted a mutational analysis of residues proposed to mediate interactions at the dimer interfaces. we demonstrate that destabilizing either int ... | 2010 | 20525790 |
| performance of the oxoid m.i.c.evaluator strips compared with the etest assay and bsac agar dilution. | the oxoid m.i.c.evaluator (m.i.c.e; thermo fisher scientific) comprises an antibiotic gradient on a plastic support. we compared its performance with etest--a similar product--using bsac agar dilution as a reference. | 2010 | 20562429 |
| occurrence and sequence of sphaeroides heme protein and diheme cytochrome c in purple photosynthetic bacteria in the family rhodobacteraceae. | sphaeroides heme protein (shp) was discovered in the purple photosynthetic bacterium, rhodobacter sphaeroides, and is the only known c-type heme protein that binds oxygen. although initially not believed to be widespread among the photosynthetic bacteria, the gene has now been found in more than 40 species of proteobacteria and generally appears to be regulated. rb. sphaeroides is exceptional in not having regulatory genes associated with the operon. we have thus analyzed additional purple bacte ... | 2010 | 20587053 |
| campylobacter fetus translocation across caco-2 cell monolayers. | campylobacter fetus is a recognized pathogen of cattle and sheep, though human infection has also been reported. ingestion of contaminated food or water is a proposed route of transmission for both humans and animals. the subsequent detection of the organism from extra-intestinal and systemic locations implies an ability to translocate across epithelial barriers. to determine how c. fetus disseminates from the intestine, caco-2 cells cultured on porous membrane supports, were used as model intes ... | 2010 | 20600794 |
| development of loop-mediated isothermal amplification and pcr assays for rapid and simple detection of campylobacter fetus subsp. venerealis. | campylobacter fetus is divided into cfv and cff. because cfv causes bovine genital campylobacteriosis, differentiation of the two subspecies is essential to the implementation of efficient cfv control and eradication programs. we have developed lamp and duplex pcr assays for rapid and simple detection of cfv. the lamp assay correctly detected 7 cfv strains and did not detect 53 cff, 35 non-fetus campylobacter and 25 non-campylobacter strains. the pcr assay successfully differentiated the two sub ... | 2010 | 20618686 |
| antibody-based sensors: principles, problems and potential for detection of pathogens and associated toxins. | antibody-based sensors permit the rapid and sensitive analysis of a range of pathogens and associated toxins. a critical assessment of the implementation of such formats is provided, with reference to their principles, problems and potential for 'on-site' analysis. particular emphasis is placed on the detection of foodborne bacterial pathogens, such as escherichia coli and listeria monocytogenes, and additional examples relating to the monitoring of fungal pathogens, viruses, mycotoxins, marine ... | 2009 | 22408533 |
| rapid identification of bacterial pathogens using a pcr- and microarray-based assay. | during the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. we have developed a method based on a modified broad-range pcr and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. the broad-range pcr primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrb and pare. the primer design a ... | 2009 | 19664269 |
| campylobacter cholecystitis. | there are 13 cases of campylobacter cholecystitis reported so far in the medical literature. among them, only 4 patients had diarrhea. we report another case of acalculous cholecystitis in a setting of campylobacter enteritis. the case report is followed by a literature review regarding this rare condition. | 2009 | 19960123 |
| rapid evolution and the importance of recombination to the gastroenteric pathogen campylobacter jejuni. | responsible for the majority of bacterial gastroenteritis in the developed world, campylobacter jejuni is a pervasive pathogen of humans and animals, but its evolution is obscure. in this paper, we exploit contemporary genetic diversity and empirical evidence to piece together the evolutionary history of c. jejuni and quantify its evolutionary potential. our combined population genetics-phylogenetics approach reveals a surprising picture. campylobacter jejuni is a rapidly evolving species, subje ... | 2009 | 19008526 |
| rapid evolution and the importance of recombination to the gastroenteric pathogen campylobacter jejuni. | responsible for the majority of bacterial gastroenteritis in the developed world, campylobacter jejuni is a pervasive pathogen of humans and animals, but its evolution is obscure. in this paper, we exploit contemporary genetic diversity and empirical evidence to piece together the evolutionary history of c. jejuni and quantify its evolutionary potential. our combined population genetics-phylogenetics approach reveals a surprising picture. campylobacter jejuni is a rapidly evolving species, subje ... | 2009 | 19008526 |
| the biofilm forming potential of bacterial species in the genus campylobacter. | the biofilm forming abilities of 16 strains representative of 14 of the 16 species comprising the genus campylobacter were determined on glass, stainless steel, and polystyrene plastic. the formation of biofilms has been suggested as a means by which campylobacter is able to persist within an inhospitable environment. of the eight microaerophilic campylobacter species, including two strains each of campylobacter jejuni and campylobacter fetus, only c. jejuni strain 81-176 reliably produced a vis ... | 2009 | 19028304 |
| increased tlr4 expression in murine placentas after oral infection with periodontal pathogens. | maternal periodontitis has emerged as a putative risk factor for preterm births in humans. the periodontitis-associated dental biofilm is thought to serve as an important source of oral bacteria and related virulence factors that hematogenously disseminate and affect the fetoplacental unit; however the underlying biological mechanisms are yet to be fully elucidated. this study hypothesized that an oral infection with the human periodontal pathogens campylobacter rectus and porphyromonas gingival ... | 2009 | 19101032 |
| increased tlr4 expression in murine placentas after oral infection with periodontal pathogens. | maternal periodontitis has emerged as a putative risk factor for preterm births in humans. the periodontitis-associated dental biofilm is thought to serve as an important source of oral bacteria and related virulence factors that hematogenously disseminate and affect the fetoplacental unit; however the underlying biological mechanisms are yet to be fully elucidated. this study hypothesized that an oral infection with the human periodontal pathogens campylobacter rectus and porphyromonas gingival ... | 2009 | 19101032 |
| development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of campylobacter fetus. | we developed a sensitive and rapid loop-mediated isothermal amplification (lamp) assay for detection of campylobacter fetus. this assay provides simpler and more rapid detection of c. fetus than conventional biochemical and pcr assays. the assay correctly detected 60 c. fetus strains but not 55 non-fetuscampylobacter and 30 non-campylobacter strains. the sensitivity of the lamp assay in pure cultures and in a spiked bovine liver specimen was 10-fold more sensitive than that of the conventional p ... | 2009 | 19128899 |
| campylobacter fetus-associated epidural abscess and bacteremia. | campylobacter fetus has been isolated as an infrequent cause of abscesses. we report a case of campylobacter fetus epidural abscess and bacteremia in a debilitated elderly man who had a fatal outcome. | 2009 | 19144805 |
| production, characterization and determination of the real catalytic properties of the putative 'succinate dehydrogenase' from wolinella succinogenes. | both the genomes of the epsilonproteobacteria wolinella succinogenes and campylobacter jejuni contain operons (sdhabe) that encode for so far uncharacterized enzyme complexes annotated as 'non-classical' succinate:quinone reductases (sqrs). however, the role of such an enzyme ostensibly involved in aerobic respiration in an anaerobic organism such as w. succinogenes has hitherto been unknown. we have established the first genetic system for the manipulation and production of a member of the non- ... | 2009 | 19170876 |
| isolation of campylobacter fetus subspecies fetus in a two-year-old quarterhorse with chronic diarrhea of an undetermined etiology. | a 2-year-old quarterhorse was evaluated for chronic diarrhea and weight loss of 5 weeks duration after numerous diagnostic tests failed to identify an underlying cause. historically, the horse was housed at pasture where human household waste vehicles were routinely cleaned and the effluent could run onto the field. physical examination revealed poor body condition and frequent high-volume diarrhea. diagnostic testing for salmonella spp., endoparasites, cryptosporidium spp., clostridium spp., an ... | 2009 | 19286512 |
| molecular epidemiology and characterization of campylobacter spp. isolated from wild bird populations in northern england. | campylobacter infections have been reported at prevalences ranging from 2 to 50% in a range of wild bird species, although there have been few studies that have investigated the molecular epidemiology of campylobacter spp. consequently, whether wild birds are a source of infection in humans or domestic livestock or are mainly recipients of domestic animal strains and whether separate cycles of infection occur remain unknown. to address these questions, serial cross-sectional surveys of wild bird ... | 2009 | 19286781 |
| [isolation of campylobacter fetus from persons with obstetric-gynecological infections]. | campylobacter fetus subspecies fetus is a cause of different obstetric-gynecological diseases. it is a first time when rate of infection with campylobacter was studied and connection between the infection and development of chronic gynecologic diseases and pathology of labor was established. bacteria were isolated and identified in 36.0% +/- 0.7 of studied women admitted to inpatient clinics. it was established that campylobacter fetus subspecies fetus can cause abnormalities in placenta functio ... | 2009 | 19338242 |
| comparative studies of campylobacter jejuni genomic diversity reveal the importance of core and dispensable genes in the biology of this enigmatic food-borne pathogen. | mlst, dna microarrays, and genome sequencing has allowed for a greater understanding of the metabolic capacity and epidemiology of campylobacter jejuni. while strain-specific genes may provide an isolate a selective advantage in environments and contribute to the organism's pathogenicity, recent work indicates that c. jejuni pathogenicity is dictated by variations in the nucleotide sequence of core genes. challenges facing c. jejuni researchers include determining (a) the degree to which genomic ... | 2009 | 19346123 |
| characterization of two homologous disulfide bond systems involved in virulence factor biogenesis in uropathogenic escherichia coli cft073. | disulfide bond (dsb) formation is catalyzed by disulfide bond proteins and is critical for the proper folding and functioning of secreted and membrane-associated bacterial proteins. uropathogenic escherichia coli (upec) strains possess two paralogous disulfide bond systems: the well-characterized dsbab system and the recently described dsbli system. in the dsbab system, the highly oxidizing dsba protein introduces disulfide bonds into unfolded polypeptides by donating its redox-active disulfide ... | 2009 | 19376849 |
| improving protein extraction and separation methods for investigating the metaproteome of anaerobic benzene communities within sediments. | btex compounds such as benzene are frequent soil and groundwater contaminants that are easily biodegraded under oxic conditions by bacteria. in contrast, benzene is rather recalcitrant under anaerobic conditions. the analysis of anoxic degradation is often hampered by difficult sampling conditions, limited amounts of biomass and interference of matrix compounds with proteomic approaches. in order to improve the procedure for protein extraction we established a scheme consisting of the following ... | 2009 | 19381451 |
| [invasive disease due to campylobacter fetus in an immunocompetent infant]. | 2009 | 19409252 | |
| genomic analysis of campylobacter fetus subspecies: identification of candidate virulence determinants and diagnostic assay targets. | campylobacter fetus subspecies venerealis is the causative agent of bovine genital campylobacteriosis, asymptomatic in bulls the disease is spread to female cattle causing extensive reproductive loss. the microbiological and molecular differentiation of c. fetus subsp. venerealis from c. fetus subsp. fetus is extremely difficult. this study describes the analysis of the available c. fetus subsp. venerealis azul-94 strain genome (approximately 75-80%) to identify elements exclusively found in c. ... | 2009 | 19422718 |
| bilateral internal iliac artery aneurysm infected with campylobacter fetus. | this is a case report of a patient with campylobacter fetus involving bilateral internal iliac artery aneurysms. the patient was treated successfully by ligation of the bilateral iliac artery aneurysms and antibiotics. according to a review of the english-language medical literature, this was the first such patient to be reported. a 69-year-old african-american male presented with a past medical history of repair of a 6.6 cm abdominal aortic aneurysm. it had been repaired with a dacron bifurcate ... | 2009 | 19698305 |
| the dif/xer recombination systems in proteobacteria. | in e. coli, 10 to 15% of growing bacteria produce dimeric chromosomes during dna replication. these dimers are resolved by xerc and xerd, two tyrosine recombinases that target the 28-nucleotide motif (dif) associated with the chromosome's replication terminus. in streptococci and lactococci, an alternative system is composed of a unique, xer-like recombinase (xers) genetically linked to a dif-like motif (dif(sl)) located at the replication terminus. preliminary observations have suggested that t ... | 2009 | 19727445 |
| functional analysis of the helicobacter pylori flagellar switch proteins. | helicobacter pylori uses flagellum-mediated chemotaxis to promote infection. bacterial flagella change rotational direction by changing the state of the flagellar motor via a subcomplex referred to as the switch. intriguingly, the h. pylori genome encodes four switch complex proteins, flim, flin, fliy, and flig, instead of the more typical three of escherichia coli or bacillus subtilis. our goal was to examine whether and how all four switch proteins participate in flagellation. previous work de ... | 2009 | 19767432 |
| long-term survival of campylobacter jejuni at low temperatures is dependent on polynucleotide phosphorylase activity. | campylobacter jejuni is a leading cause of bacterial gastroenteritis worldwide. infection generally occurs after ingestion of contaminated poultry products, usually conserved at low temperatures. the mechanisms promoting survival of c. jejuni in the cold remain poorly understood despite several investigations. the present study provides insight into the survival mechanism by establishing the involvement of polynucleotide phosphorylase (pnpase), a 3'-5' exoribonuclease with multiple biological fu ... | 2009 | 19801468 |
| campylobacter fetus cellulitis in an immunocompromised patient: case report and review of the literature. | campylobacter fetus is an opportunist gram-negative bacillus. the most frequent clinical manifestation is bacteriemia but it can also be responsable for soft tissue infections, endovascular infections, meningitis, peritonitis and thrombophlebitis. campylobacter fetus cellulitis has been described, but rarely identified in subcutaneous puncture samples. we report a case of an immunocompromised patient with campylobacter fetus bacteriemia associated with a soft tissue infection whose subcutaneous ... | 2009 | 19810424 |
| campylobacter fetus subspecies venerealis transport medium for enrichment and pcr. | 2009 | 19855114 | |
| campylobacter fetus meningitis in a patient with crohn's disease. | 2009 | 18798576 | |
| forty years' experience treating septic arteritis and vasculitis. | by their natures, septic arteritis and infected grafts present a high morbidity and mortality situation for both the patient and the treating physician. in the experience of the authors, therapy frequently includes removing the previous infected graft, use of long-term antibiotics, repeat grafting and omental wraps. when possible, aortic endografting followed by future elective primary repair may be considered an option. leaving wounds open to granulate from the 'bottom up' has been successful i ... | 2009 | 22477474 |
| synonymous and nonsynonymous polymorphisms versus divergences in bacterial genomes. | comparison of the ratio of nonsynonymous to synonymous polymorphisms within species with the ratio of nonsynonymous to synonymous substitutions between species has been widely used as a supposed indicator of positive darwinian selection, with the ratio of these 2 ratios being designated as a neutrality index (ni). comparison of genome-wide polymorphism within 12 species of bacteria with divergence from an outgroup species showed substantial differences in ni among taxa. a low level of nonsynonym ... | 2008 | 18667439 |
| evidence for the involvement of infectious agents in the pathogenesis of crohn's disease. | many advances have been made in the understanding of crohn's disease (cd) pathogenesis during the last decade. cd is currently seen as a predominantly t-lymphocyte-driven disease characterized by the presence of a complex cocktail of interacting cytokines, chemokines and other mediators produced by a variety of cell types. prevailing theories of cd pathogenesis suggest that patients' t-lymphocytes are inappropriately activated in the setting of an immune imbalance, which is itself caused by an u ... | 2008 | 18240341 |
| development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of vibrio parahaemolyticus. | vibrio parahaemolyticus is a marine seafood-borne pathogen causing gastrointestinal disorders in humans. thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) are known as major virulence determinants of v. parahaemolyticus. most v. parahaemolyticus isolates from the environment do not produce tdh or trh. total v. parahaemolyticus has been used as an indicator for control of seafood contamination toward prevention of infection. detection of total v. parahaemolyticus using conventio ... | 2008 | 18823567 |
| campylobacter fetus bloodstream infection: risk factors and clinical features. | in this paper, we report 21 cases of campylobacter fetus bloodstream infection observed in our institution over a 9-year period. the median age of the patients was 78 years. most of them (62%) had a significant underlying disease, such as diabetes, immunodeficiency or cardiovascular disease. the main clinical features were fever with (62% of cases) or without (38%) extra-intestinal symptoms. these included mycotic aneurysm of the abdominal aorta (24%) and cellulitis (19%). antibiotic treatment w ... | 2008 | 17999095 |
| wide geographical distribution of internationally rare campylobacter clones within new zealand. | during the southern hemisphere winter of 2006 new zealand experienced a significant increase in the number of reported cases of campylobacter infection. in total, 112 campylobacter isolates from eight district health boards (dhbs) located across new zealand were submitted for pfge, mlst and penner serotyping analysis. distinct clusters of campylobacter isolates were identified, several of which were composed of isolates from up to five different dhbs located on both the north and south islands o ... | 2008 | 18028582 |
| wide geographical distribution of internationally rare campylobacter clones within new zealand. | during the southern hemisphere winter of 2006 new zealand experienced a significant increase in the number of reported cases of campylobacter infection. in total, 112 campylobacter isolates from eight district health boards (dhbs) located across new zealand were submitted for pfge, mlst and penner serotyping analysis. distinct clusters of campylobacter isolates were identified, several of which were composed of isolates from up to five different dhbs located on both the north and south islands o ... | 2008 | 18028582 |
| retrospective study of campylobacter infection in a zoological collection. | little is known about the epidemiology of campylobacter spp. in wild animal populations. however, zoological collections can provide valuable insights. using records from the zoological society of london whipsnade zoo compiled between 1990 and 2003, the roles of a range of biotic and abiotic factors associated with the occurrence of campylobacteriosis were investigated. the occurrence of campylobacteriosis varied widely across host taxonomic orders. furthermore, in mammals, a combination of chan ... | 2008 | 18165368 |
| retrospective study of campylobacter infection in a zoological collection. | little is known about the epidemiology of campylobacter spp. in wild animal populations. however, zoological collections can provide valuable insights. using records from the zoological society of london whipsnade zoo compiled between 1990 and 2003, the roles of a range of biotic and abiotic factors associated with the occurrence of campylobacteriosis were investigated. the occurrence of campylobacteriosis varied widely across host taxonomic orders. furthermore, in mammals, a combination of chan ... | 2008 | 18165368 |
| arthritis risk after acute bacterial gastroenteritis. | reactive arthritis (rea) may occur from bacterial gastroenteritis. we studied the risk of arthritis after an outbreak of escherichia coli o157:h7 and campylobacter species within a regional drinking water supply to examine the relationship between the severity of acute diarrhoea and subsequent symptoms of arthritis. | 2008 | 18184664 |
| moraxella osloensis gene expression in the slug host deroceras reticulatum. | the bacterium moraxella osloensis is a mutualistic symbiont of the slug-parasitic nematode phasmarhabditis hermaphrodita. in nature, p. hermaphrodita vectors m. osloensis into the shell cavity of the slug host deroceras reticulatum in which the bacteria multiply and kill the slug. as m. osloensis is the main killing agent, genes expressed by m. osloensis in the slug are likely to play important roles in virulence. studies on pathogenic interactions between bacteria and lower order hosts are few, ... | 2008 | 18226222 |
| campylobacter fetus infection of an internal cardioverter defibrillator. | infection is a serious complication post implantation of an implantable cardiac defibrillator (icd). we report a case of a 77-year-old man who had an icd generator change complicated by a hematoma. the generator became infected at the time of campylobacter fetus bacteremia. the icd was removed and a new device was implanted without complication. | 2008 | 18233985 |
| the macaque gut microbiome in health, lentiviral infection, and chronic enterocolitis. | the vertebrate gut harbors a vast community of bacterial mutualists, the composition of which is modulated by the host immune system. many gastrointestinal (gi) diseases are expected to be associated with disruptions of host-bacterial interactions, but relatively few comprehensive studies have been reported. we have used the rhesus macaque model to investigate forces shaping gi bacterial communities. we used dna bar coding and pyrosequencing to characterize 141,000 sequences of 16s rrna genes ob ... | 2008 | 18248093 |
| development of a cytolethal distending toxin (cdt) gene-based species-specific multiplex pcr assay for the detection and identification of campylobacter jejuni, campylobacter coli and campylobacter fetus. | a cytolethal distending toxin (cdt) gene-based species-specific multiplex pcr assay for the detection of cdta, cdtb or cdtc gene of campylobacter jejuni, campylobacter coli or campylobacter fetus, respectively, was developed and evaluated with 76 campylobacter strains belonging to seven different species and 131 other bacterial strains of eight different genera. the cdta, cdtb or cdtc gene of c. jejuni, c. coli or c. fetus, respectively, could be successfully amplified using the corresponding se ... | 2008 | 18248435 |
| development and evaluation of immunochromatographic assay for simple and rapid detection of campylobacter jejuni and campylobacter coli in human stool specimens. | an immunochromatographic assay (campy-ica) using a newly generated single monoclonal antibody against a 15-kda cell surface protein of campylobacter jejuni was developed. when cell suspensions of 86 c. jejuni strains and 27 campylobacter coli strains were treated with a commercially available bacterial protein extraction reagent and the resulting extracts were tested with the campy-ica, they all yielded positive results. the minimum detectable limits for the c. jejuni strains ranged from 1.8 x 1 ... | 2008 | 18256225 |
| development of a strain-specific molecular method for quantitating individual campylobacter strains in mixed populations. | the identification of sites resulting in cross-contamination of poultry flocks in the abattoir and determination of the survival and persistence of campylobacters at these sites are essential for the development of intervention strategies aimed at reducing the microbial burden on poultry at retail. a novel molecule-based method, using strain- and genus-specific oligonucleotide probes, was developed to detect and enumerate specific campylobacter strains in mixed populations. strain-specific oligo ... | 2008 | 18281428 |
| emergence of a tetracycline-resistant campylobacter jejuni clone associated with outbreaks of ovine abortion in the united states. | campylobacter infection is one of the major causes of ovine abortions worldwide. historically, campylobacter fetus subsp. fetus was the major cause of campylobacter-associated abortion in sheep; however, campylobacter jejuni is increasingly associated with sheep abortions. we examined the species distribution, genotypes, and antimicrobial susceptibilities of abortion-associated campylobacter isolates obtained from multiple lambing seasons on different farms in iowa, idaho, south dakota, and cali ... | 2008 | 18322054 |
| evaluation of 16s rrna gene-based pcr assays for genus-level identification of helicobacter species. | the inclusivity, exclusivity, and detection limit of six 16s rrna gene-based helicobacter genus-specific pcr assays were examined. five out of six assays were 100% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5%). the clinical detection limit varied between 10(3) and 1 viable bacterial cell per reaction mixture. | 2008 | 18337392 |
| sequence variability of campylobacter temperate bacteriophages. | prophages integrated within the chromosomes of campylobacter jejuni isolates have been demonstrated very recently. prior work with campylobacter temperate bacteriophages, as well as evidence from prophages in other enteric bacteria, suggests these prophages might have a role in the biology and virulence of the organism. however, very little is known about the genetic variability of campylobacter prophages which, if present, could lead to differential phenotypes in isolates carrying the phages ve ... | 2008 | 18366706 |
| fibronectin enhances campylobacter fetus interaction with extracellular matrix components and int 407 cells. | campylobacter fetus is a recognized pathogen of cattle and sheep that can also infect humans. no adhesins specific for c. fetus have to date been identified; however, bacterial attachment is essential to establish an infecting population. scanning electron microscopy revealed c. fetus attachment to the serosal surface of human colonic biopsy explants, a location consistent with the presence of the extracellular matrix (ecm). to determine whether the ecm mediated c. fetus adherence, 7 c. fetus st ... | 2008 | 18388970 |
| infected aneurysms of bilateral deep femoral arteries due to campylobacter fetus subspecies fetus. | infected aneurysms due to campylobacter fetus subspecies fetus have rarely been reported. here, we describe the first case of infected aneurysm of bilateral deep femoral arteries due to c. fetus fetus. we successfully treated this case by administration of antibiotics effective for c. fetus fetus and bilateral obturator bypass with complete resection of the infected aneurysms. the aneurysmal wall culture disclosed the presence of c. fetus fetus in a microaerobic atmosphere after the operation. a ... | 2008 | 18466824 |
| sensitive and rapid detection of cholera toxin-producing vibrio cholerae using a loop-mediated isothermal amplification. | vibrio cholerae is widely acknowledged as one of the most important waterborne pathogen causing gastrointestinal disorders. cholera toxin (ct) is a major virulence determinant of v. cholerae. detection of ct-producing v. cholerae using conventional culture-, biochemical- and immunological-based assays is time-consuming and laborious, requiring more than three days. thus, we developed a novel and highly specific loop-mediated isothermal amplification (lamp) assay for the sensitive and rapid detec ... | 2008 | 18547441 |
| [case of bilateral subdural empyema complicating campylobacter fetus subspecies fetus meningitis]. | campylobacter fetus subspecies fetus (c. fetus) is a relatively rare cause of meningitis, with only 3 cases of c. fetus subdural empyema reported to date. we experienced a case of bilateral subdural empyema complicating c. fetus meningitis. a 51-year-old man was found lying unconscious on a street and was brought to the hospital in an ambulance. he had no past history of any underlying disease but he had suffered head trauma approximately 1 month prior to this episode. on admission, he had high ... | 2008 | 18567362 |
| identification of a gene encoding a transporter essential for utilization of c4 dicarboxylates in corynebacterium glutamicum. | the corynebacterium glutamicum r genome contains a total of eight genes encoding proteins with sequence similarity to c4-dicarboxylate transporters identified from other bacteria. three of the genes encode proteins within the dicarboxylate/amino acid:cation symporter (daacs) family, another three encode proteins within the tripartite atp-independent periplasmic transporter family, and two encode proteins within the divalent anion:na+ symporter (dass) family. we observed that a mutant strain defi ... | 2008 | 18586971 |
| design and construction of a double inversion recombination switch for heritable sequential genetic memory. | inversion recombination elements present unique opportunities for computing and information encoding in biological systems. they provide distinct binary states that are encoded into the dna sequence itself, allowing us to overcome limitations posed by other biological memory or logic gate systems. further, it is in theory possible to create complex sequential logics by careful positioning of recombinase recognition sites in the sequence. | 2008 | 18665232 |
| unveiling novel reco distant orthologues involved in homologous recombination. | the generation of a reca filament on single-stranded dna is a critical step in homologous recombination. two main pathways leading to the formation of the nucleofilament have been identified in bacteria, based on the protein complexes mediating reca loading: recbcd (addab) and recfor. many bacterial species seem to lack some of the components involved in these complexes. the current annotation of the helicobacter pylori genome suggests that this highly diverse bacterial pathogen has a reduced se ... | 2008 | 18670631 |
| necessary corrections to the approved lists of bacterial names according to rule 40d (formerly rule 46). opinion 86. | the judicial commission affirms that, according to rule 40d, formerly rule 46, of the bacteriological code, the authorship of a number of subspecies names included on the approved lists of bacterial names must be corrected. these names are acetobacter aceti subsp. aceti, acetobacter pasteurianus subsp. pasteurianus, bacteroides melaninogenicus subsp. melaninogenicus, campylobacter fetus subsp. fetus, mycobacterium chelonae subsp. chelonae, propionibacterium freudenreichii subsp. freudenreichii, ... | 2008 | 18676492 |
| [infected abdominal aortic aneurysm from campylobacter fetus bacteremia]. | 2008 | 18681158 | |
| aortoiliac aneurysms infected by campylobacter fetus. | few reports of aortoiliac aneurysms infected by campylobacter fetus are available. we report five cases and review previous reports, with a view to describing the clinical pattern, treatment options, and outcome of this infection. | 2008 | 18692356 |
| campylobacter bacteremia: clinical features and factors associated with fatal outcome. | campylobacter bacteremia is uncommon. the influence of underlying conditions and of the impact of antibiotics on infection outcome are not known. | 2008 | 18699745 |
| occurrence and characterization of campylobacter in the brazilian production and processing of broilers. | twenty-two commercial broiler flocks and their carcasses, totaling 546 samples (450 collected from a poultry farm and 96 from a slaughterhouse), were surveyed for the presence of campylobacter. the positive results for campylobacter among the analyzed samples were homogeneous, yielding 81.8% for cecal droppings, 80.9% for feces, and 80.4% for cloacal swabs. pre-enrichment and direct plating showed that 77.85% and 81.8% of cloacal swabs, respectively, were positive for campylobacter compared to 9 ... | 2008 | 19166063 |
| influence of campylobacter fetus subsp. fetus on ram sperm cell quality. | campylobacter fetus subsp. fetus infection can occur in female sheep, causing infertility or abortion. despite extensive research on the effect of these bacteria on female fertility, little research has been done on the influence of c. fetus subsp. fetus on the male factor. our objective was to examine the influence of c. fetus subsp. fetus on ram sperm. motility index, percentage of live spermatozoa, mean alphat value (an indication of the chromatin stability of the sperm cell) and percentage o ... | 2008 | 18927420 |
| demographic determinants for campylobacter infection in england and wales: implications for future epidemiological studies. | despite a significant public health burden the epidemiology of human campylobacter infection remains blurred. the identification of demographic determinants for campylobacter infection is therefore essential for identifying potential areas for intervention. demographic data from an active, population-based sentinel surveillance system for campylobacter infection (from 2000 until 2003, n=15 907) were compared with appropriate denominator data from the 2001 united kingdom census. incidence was hig ... | 2008 | 19000328 |
| campylobacter infection as a trigger for guillain-barré syndrome in egypt. | most studies of campylobacter infection triggering guillain-barré syndrome (gbs) are conducted in western nations were campylobacter infection and immunity is relatively rare. in this study, we explored campylobacter infections, campylobacter serotypes, autoantibodies to gangliosides, and gbs in egypt, a country where campylobacter exposure is common. | 2008 | 19002255 |
| the complete genome sequence and analysis of the epsilonproteobacterium arcobacter butzleri. | arcobacter butzleri is a member of the epsilon subdivision of the proteobacteria and a close taxonomic relative of established pathogens, such as campylobacter jejuni and helicobacter pylori. here we present the complete genome sequence of the human clinical isolate, a. butzleri strain rm4018. | 2007 | 18159241 |
| s-layer anchoring and localization of an s-layer-associated protease in caulobacter crescentus. | the s-layer of the gram-negative bacterium caulobacter crescentus is composed of a single protein, rsaa, that is secreted and assembled into a hexagonal crystalline array that covers the organism. despite the widespread occurrence of comparable bacterial s-layers, little is known about s-layer attachment to cell surfaces, especially for gram-negative organisms. having preliminary indications that the n terminus of rsaa anchors the monomer to the cell surface, we developed an assay to distinguish ... | 2007 | 17209028 |
| comparison of disk diffusion and agar dilution methods for erythromycin and ciprofloxacin susceptibility testing of campylobacter jejuni subsp. jejuni. | disk diffusion was a reliable, easy, and inexpensive method for testing the susceptibility of campylobacter jejuni to erythromycin (215 susceptible and 45 resistant isolates) and to ciprofloxacin (154 susceptible, two intermediate, and 124 resistant isolates) using, respectively, an erythromycin disk and ciprofloxacin and nalidixic acid disks. | 2007 | 17261628 |
| specific detection of campylobacter jejuni from faeces using single nucleotide polymorphisms. | specimens of human faeces were tested by a rapid strategy for detection of campylobacter jejuni lineages by the presence of specific single nucleotide polymorphisms (snps) based on the c. jejuni multi locus sequence typing (mlst) scheme. this strategy was derived from analysis of the mlst databases to identify clonal complex specific snps followed by the design of real-time pcr assays to enable identification of six major c. jejuni clonal complexes associated with cases of human infection. the o ... | 2007 | 17109769 |
| specific detection of campylobacter jejuni from faeces using single nucleotide polymorphisms. | specimens of human faeces were tested by a rapid strategy for detection of campylobacter jejuni lineages by the presence of specific single nucleotide polymorphisms (snps) based on the c. jejuni multi locus sequence typing (mlst) scheme. this strategy was derived from analysis of the mlst databases to identify clonal complex specific snps followed by the design of real-time pcr assays to enable identification of six major c. jejuni clonal complexes associated with cases of human infection. the o ... | 2007 | 17109769 |
| temperate bacteriophages affect pulsed-field gel electrophoresis patterns of campylobacter jejuni. | the recently sequenced genome of campylobacter jejuni rm1221 revealed the presence of three integrated bacteriophage-like elements. in this study, genes from the first element, a mu-like bacteriophage, were amplified by pcr and used to probe pulsed-field gels of clinical c. jejuni strains obtained from a waterborne outbreak (ontario, canada, 2000). these highly similar strains differed only by their pulsed-field gel electrophoresis (pfge) patterns due to an apparent insertion or deletion of a 40 ... | 2007 | 17135440 |
| temperate bacteriophages affect pulsed-field gel electrophoresis patterns of campylobacter jejuni. | the recently sequenced genome of campylobacter jejuni rm1221 revealed the presence of three integrated bacteriophage-like elements. in this study, genes from the first element, a mu-like bacteriophage, were amplified by pcr and used to probe pulsed-field gels of clinical c. jejuni strains obtained from a waterborne outbreak (ontario, canada, 2000). these highly similar strains differed only by their pulsed-field gel electrophoresis (pfge) patterns due to an apparent insertion or deletion of a 40 ... | 2007 | 17135440 |
| capa, an autotransporter protein of campylobacter jejuni, mediates association with human epithelial cells and colonization of the chicken gut. | two putative autotransporter proteins, capa and capb, were identified in silico from the genome sequence of campylobacter jejuni nctc11168. the genes encoding each protein contain homopolymeric tracts, suggestive of phase variation mediated by a slipped-strand mispairing mechanism; in each case the gene sequence contained frameshifts at these positions. the c-terminal two-thirds of the two genes, as well as a portion of the predicted signal peptides, were identical; the remaining n-terminal port ... | 2007 | 17172331 |
| capa, an autotransporter protein of campylobacter jejuni, mediates association with human epithelial cells and colonization of the chicken gut. | two putative autotransporter proteins, capa and capb, were identified in silico from the genome sequence of campylobacter jejuni nctc11168. the genes encoding each protein contain homopolymeric tracts, suggestive of phase variation mediated by a slipped-strand mispairing mechanism; in each case the gene sequence contained frameshifts at these positions. the c-terminal two-thirds of the two genes, as well as a portion of the predicted signal peptides, were identical; the remaining n-terminal port ... | 2007 | 17172331 |
| oxygen reactivity of both respiratory oxidases in campylobacter jejuni: the cydab genes encode a cyanide-resistant, low-affinity oxidase that is not of the cytochrome bd type. | the microaerophilic bacterium campylobacter jejuni is a significant food-borne pathogen and is predicted to possess two terminal respiratory oxidases with unknown properties. inspection of the genome reveals an operon (cydab) apparently encoding a cytochrome bd-like oxidase homologous to oxidases in escherichia coli and azotobacter vinelandii. however, c. jejuni cells lacked all spectral signals characteristic of the high-spin hemes b and d of these oxidases. mutation of the cydab operon of c. j ... | 2007 | 17172349 |
| oxygen reactivity of both respiratory oxidases in campylobacter jejuni: the cydab genes encode a cyanide-resistant, low-affinity oxidase that is not of the cytochrome bd type. | the microaerophilic bacterium campylobacter jejuni is a significant food-borne pathogen and is predicted to possess two terminal respiratory oxidases with unknown properties. inspection of the genome reveals an operon (cydab) apparently encoding a cytochrome bd-like oxidase homologous to oxidases in escherichia coli and azotobacter vinelandii. however, c. jejuni cells lacked all spectral signals characteristic of the high-spin hemes b and d of these oxidases. mutation of the cydab operon of c. j ... | 2007 | 17172349 |
| management of an abdominal aortic aneurysm infected with campylobacter fetus: a case report. | we present a rare case of an abdominal aortic aneurysm (aaa) infected with campylobacter fetus. the patient presented with abdominal pain and leukocytosis, without a palpable aaa. computed tomography (ct) of the abdomen showed a 3.1 x 3.0 cm infrarenal abdominal aneurysm with an extra-aortic fluid collection. at surgery, an in situ graft was placed. intraoperative aortic wall cultures grew pansensitive c. fetus, and blood cultures remained negative. at 9-month follow-up, the patient was doing we ... | 2007 | 17349352 |
| campylobacter reactive arthritis: a systematic review. | to review the literature on the epidemiology of campylobacter-associated reactive arthritis (rea). | 2007 | 17360026 |
| thermophilic campylobacter spp. in salad vegetables in malaysia. | the main aim of this study was to combine the techniques of most probable number (mpn) and polymerase chain reaction (pcr) for quantifying the prevalence and numbers of campylobacter spp. in ulam, a popular malaysian salad dish, from a traditional wet market and two modern supermarkets in selangor, malaysia. a total of 309 samples of raw vegetables which are used in ulam were examined in the study. the prevalences of campylobacters in raw vegetables were, for supermarket i, campylobacter spp., 5 ... | 2007 | 17399832 |
| in vitro activities of cg400549, a novel fabi inhibitor, against recently isolated clinical staphylococcal strains in korea. | the in vitro activities of cg400549, a novel fabi inhibitor, were compared to those of linezolid and commonly used antimicrobials against recent bacterial isolates. cg400549 had an mic(90) of 0.5 microg/ml for staphylococcus aureus strains and was more potent than either linezolid or vancomycin. | 2007 | 17420210 |
| [campylobacter fetus bacteremia and cellulitis complicating a venous access port infection in an hiv infected patient]. | campylobacter fetus subspecies fetus is an opportunist gram-negative bacillus, which is known to be a cause of systemic infections, mainly in immunocompromised patients. we report a c. fetus bacteremia and cellulitis complicating a venous access port infection in a patient with acquired immunodeficiency syndrome (aids). this bacillus seems to have a predilection for the vascular endothelium and its isolation is difficult. physicians should be aware of c. fetus infection in patients with vascular ... | 2007 | 17446026 |