Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| junin virus activity in rodents from endemic and nonendemic loci in central argentina. | small mammals were trapped during a 21-month period at 27 farm sites in 15 localities within and beyond the known endemic area for argentine hemorrhagic fever (ahf). prevalence of junin virus (jv) was assessed by antigen-capture enzyme immunoassay (elisa) on samples of body fluids and/or organs from 3, 282 captured rodents. infection in rodent populations was variable (0-3.7%) among localities but, in all cases, was lower than previously reported rates. overall prevalence was 1.4% in the ahf epi ... | 1991 | 1650148 |
| toward a vaccine against argentine hemorrhagic fever. | a vaccine against argentine hemorrhagic fever, the "mal de los rastrojos" of the pampas, has been a dream of physicians and scientists involved with the disease since its recognition in the 1950s. several killed and live immunogens have been produced and tested in pursuit of this goal, none of which has proved suitable for widespread human use. recently, a new live-attenuated junin virus vaccine, candid #1, was developed through a cooperative international effort. testing conducted to date indic ... | 1991 | 1654168 |
| molecular organization of junin virus s rna: complete nucleotide sequence, relationship with other members of the arenaviridae and unusual secondary structures. | in this study, overlapping cdna clones covering the entire s rna molecule of junin virus, an arenavirus that causes argentine haemorrhagic fever, were generated. the complete sequence of this 3400 nucleotide rna was determined using the dideoxynucleotide chain termination method. the nucleocapsid protein (n) and the glycoprotein precursor (gpc) genes were identified as two non-overlapping open reading frames of opposite polarity, encoding primary translation products of 564 and 481 amino acids, ... | 1991 | 1654373 |
| experimental infection of suckling mice with a host range mutant of junin virus. | experimental infection of three mouse strains with a non-pathogenic mutant of junin virus named cl67 was compared with respect to the parental xjcl3 strain. after intracerebral (ic) or intraperitoneal inoculation, xjcl3 was highly virulent for 2 day-old c3h/hej, of1, and balb/cj mouse strains, whereas its derivative cl67 was attenuated. survival of the cl67-infected mouse was associated with a restricted replication at the site of inoculation which would impair spread of virus. thus, the reduced ... | 1991 | 1658215 |
| [lysosomotropic compounds inhibiting the multiplication of junin virus]. | a number of lysosomotropic compounds of diverse chemical structure, ammonium chloride, procaine and chlorpheniramine, were found to inhibit the infection of vero cells by junín virus. viral replication was almost totally inhibited by 15 mm ammonium chloride, when added either before or within the first hour of infection and a significant inhibition (97.8%) was observed when it was added 8 hours after infection. these results agree with a model which postulates that arenavirus entry occurs by rec ... | 1991 | 1667699 |
| rapid vascular clearance of two strains of junin virus in calomys musculinus: selective macrophage clearance. | clearance of junin (jun) virus strains with different virulence for calomys musculinus (cm) was followed using the candid #1 virulent and cbafha 5069 attenuated strains. in addition, virulent virus albino mice (am) were included as control host and venezuelan equine encephalitis (vee-vi) virus as control virus. the virus inoculum (vo) and the blood samples (vt) obtained at different times post-inoculation (p.i.) were titrated on vero cells and the cleared plaque forming-units (pfu) were calculat ... | 1991 | 1681712 |
| rapid vascular clearance of two strains of junin virus in calomys musculinus: selective macrophage clearance. | clearance of junin (jun) virus strains with different virulence for calomys musculinus (cm) was followed using the candid #1 virulent and cbafha 5069 attenuated strains. in addition, virulent virus albino mice (am) were included as control host and venezuelan equine encephalitis (vee-vi) virus as control virus. the virus inoculum (vo) and the blood samples (vt) obtained at different times post-inoculation (p.i.) were titrated on vero cells and the cleared plaque forming-units (pfu) were calculat ... | 1991 | 1681712 |
| a triple staining procedure to evaluate phagocytic role of differentiated astrocytes. | to determine whether phagocytic activity is affected by a viral infection known to induce astrocyte differentiation, a triple procedure (pap labeling for gfap, pas reaction for added baker's yeast cells and hematoxylin for nuclear staining of the whole monolayer) was applied to junin virus-inoculated cultures, as well as matched controls. the three-step staining simplified yeast cell count for subsequent statistical analysis, which discerned preferential uptake by differentiated rather than imma ... | 1991 | 1724055 |
| microgeographic distribution of allele frequencies in populations of calomys laucha (rodentia, cricetidae). | the microgeographic differentiation of allelic frequencies was analysed in two population samples of calomys laucha, collected in a grid set in cultivated fields at laguna larga (córdoba province, argentina). one of the samples (n = 79) was obtained in may (autumn), when the population density is at a maximum, and the other (n = 85) in november (spring), at the onset of reproductive activity. fst values for the november sample show significant differences between the six groups over which the sa ... | 1991 | 1880049 |
| microgeographic distribution of allele frequencies in populations of calomys laucha (rodentia, cricetidae). | the microgeographic differentiation of allelic frequencies was analysed in two population samples of calomys laucha, collected in a grid set in cultivated fields at laguna larga (córdoba province, argentina). one of the samples (n = 79) was obtained in may (autumn), when the population density is at a maximum, and the other (n = 85) in november (spring), at the onset of reproductive activity. fst values for the november sample show significant differences between the six groups over which the sa ... | 1991 | 1880049 |
| [new findings on junin virus infection in rodents inside and outside the endemic area of hemorrhagic fever in argentina]. | in conjunction with field trials for a vaccine against argentine hemorrhagic fever (ahf), small mammals were trapped during a 28-month period (1 november 1987 to 13 march 1990) in 3 epidemiologically defined areas of the central argentine pampas: northern and central buenos aires provinces were included in the ahf "historic" area, where the disease was common 15-20 years ago, but case rates are currently low; southern santa fe province is the current high-incidence area for ahf; the nonendemic a ... | 1991 | 7476104 |
| protection of guinea pigs against experimental argentine hemorrhagic fever by purified human igg: importance of elimination of infected cells. | antibody-containing plasma from patients recovered from argentine hemorrhagic fever (ahf) is of proven value in treatment of the acute disease, but the possibility of transmitting blood-borne organisms such as hiv and hepatitis virus detracts from this approach. purified human immune plasma fractions igg1,2,4, igg1,2,3,4 and f(ab')2 neutralized junin virus in vitro. igg1,2,3,4 and igg1,2,4 lysed (in the presence of complement) cells infected with junin virus, and protected infected guinea pigs f ... | 1990 | 1965845 |
| protection of guinea pigs against experimental argentine hemorrhagic fever by purified human igg: importance of elimination of infected cells. | antibody-containing plasma from patients recovered from argentine hemorrhagic fever (ahf) is of proven value in treatment of the acute disease, but the possibility of transmitting blood-borne organisms such as hiv and hepatitis virus detracts from this approach. purified human immune plasma fractions igg1,2,4, igg1,2,3,4 and f(ab')2 neutralized junin virus in vitro. igg1,2,3,4 and igg1,2,4 lysed (in the presence of complement) cells infected with junin virus, and protected infected guinea pigs f ... | 1990 | 1965845 |
| [intracerebral infection of athymic mice with an attenuated strain of junín virus]. | infection of newborn immunocompetent (nu/+) mice with the xj prototype strain of junin virus, etiological agent of argentine hemorrhagic fever, produces a lethal meningo-encephalitis due to cellular immune response. the same strain inoculated into athymic (nu/nu) mice produces an asymptomatic persistent infection. the purpose of this work was to determine the nu/nu mice response when infected with xjcl3, an attenuated junin virus strain, since this strain behaves differently to the pathogenic pr ... | 1990 | 1966306 |
| junin virus replication in peripheral blood mononuclear cells of patients with argentine haemorrhagic fever. | to study the relationship of junin virus (jv) to populations of peripheral blood mononuclear cells (pbmc) from patients with argentine haemorrhagic fever (ahf), blood samples were obtained during the acute period of disease and cultured as total, adherent, and non-adherent cell populations. jv was sequentially sought in these cell populations by using an infectious centre (ic) assay, whereas free jv in the supernatants was evaluated by plaque formation. ic were obtained in cultures of total pbmc ... | 1990 | 1975726 |
| hantavirus infection in laboratory and wild rodents in argentina. | serum samples from urban and laboratory rats, laboratory mice and wild and laboratory cricetids in argentina were tested by immunofluorescence and plaque reduction neutralization tests to investigate prevalence of anti-hantavirus antibodies. a total of 102 sera were obtained from laboratory rodents in 4 different animal-rooms, 31 from harbor rats and 30 from wild cricetids in 1985-1987. anti-hantavirus antibodies were detected in 22.5% of rattus norvegicus in 3 of the animal-rooms but harbor rat ... | 1990 | 1981381 |
| hantavirus infection in laboratory and wild rodents in argentina. | serum samples from urban and laboratory rats, laboratory mice and wild and laboratory cricetids in argentina were tested by immunofluorescence and plaque reduction neutralization tests to investigate prevalence of anti-hantavirus antibodies. a total of 102 sera were obtained from laboratory rodents in 4 different animal-rooms, 31 from harbor rats and 30 from wild cricetids in 1985-1987. anti-hantavirus antibodies were detected in 22.5% of rattus norvegicus in 3 of the animal-rooms but harbor rat ... | 1990 | 1981381 |
| a plasma inhibitor of platelet aggregation in patients with argentine hemorrhagic fever. | hemorrhage in patients with lassa fever is associated with the presence of a circulating plasma inhibitor of platelet aggregation. this study was to determine whether patients with argentine hemorrhagic fever (ahf) develop a similar inhibitor. normal platelets showed significantly weaker aggregation responses to a sub-maximal dose of adenosine diphosphate (adp) when mixed with plasma from 10 patients with ahf (mean percent of control +/- 1 se = 57.2 +/- 6.7%) compared to those mixed with plasma ... | 1990 | 2160197 |
| junin virus-induced suppressor cells are effective only on the efferent limb of the delayed-type hypersensitivity response. | junin virus (jv), the etiological agent of argentinian hemorrhagic fever, induces high mortality in the suckling balb/c mouse, which correlates with delayed-type hypersensitivity (dth)-like immune response. in contrast, the adult mouse is resistant to infection, and no dth response can be detected. an antigen-nonspecific dth suppressor t-cell activity induced by jv has been described that may be related to adult mouse survival. in this report, we present evidence supporting the inability of such ... | 1990 | 2160514 |
| method for improving accuracy of virus titration: standardization of plaque assay for junin virus. | titrating infective virus is one of the most important and common techniques in virology. however, after many years of widespread use, the parameters governing the accuracy of titration values are still not well understood. it was found that under conditions currently used for virus titration, only a small percentage of virus in the inoculum is adsorbed onto the cells and thereby detected in the titration assay. the objective of our work was to establish the conditions for a plaque assay which c ... | 1990 | 1707890 |
| [immunopathology induced in the rat by junin virus]. | intra-cerebral infection of the 10-day-old rat with the xj prototype strain of junin virus induces an immunopathological encephalitis with 100% mortality. in contrast with previous observations, our present work with antithymocyte serum (ats) demonstrates a pathological role for the cellular immune response in this experimental model. as regards ats treatment, 3 schedules were employed, the most efficient being daily 0.01 ml/g weight doses from day -1 to day +9, then +12, +14 and +16, taking day ... | 1990 | 1966503 |
| [blood parameters variation in calomys musculinus infected with junin virus, strain xjcl3]. | the aim of this study was to analyze the alterations in homeostasis induced by junin virus during acute and persistent infection of c. musculinus. virus presence in brain, hematological response and glycemia levels were evaluated. newborn c. musculinus inoculated with 4000 dl50 of junin virus, strain xjcl3 by intraperitoneal route developed a typical acute disease, with 50-70% mortality. virus was isolated from brain starting day 6 post-infection (fig. 1) and the peak titer (10(8) dl50/ml) was r ... | 1990 | 1966622 |
| [blood parameters variation in calomys musculinus infected with junin virus, strain xjcl3]. | the aim of this study was to analyze the alterations in homeostasis induced by junin virus during acute and persistent infection of c. musculinus. virus presence in brain, hematological response and glycemia levels were evaluated. newborn c. musculinus inoculated with 4000 dl50 of junin virus, strain xjcl3 by intraperitoneal route developed a typical acute disease, with 50-70% mortality. virus was isolated from brain starting day 6 post-infection (fig. 1) and the peak titer (10(8) dl50/ml) was r ... | 1990 | 1966622 |
| a refined complement-enhanced neutralization test for detecting antibodies to junin virus. | a refined, complement-enhanced, plaque-reduction neutralization test was developed for measuring neutralizing antibodies against junin (argentine hemorrhagic fever) virus. the assay measured neutralizing antibodies after natural as well as vaccine-induced junin virus infections. among vaccinated individuals, titers were 2-4-fold higher than those obtained with conventional assays, without loss of specificity. enhanced sensitivity was achieved by using a standardized complement source (vs human o ... | 1990 | 2170437 |
| contrasuppressor cells induced by junin virus. | intracerebral inoculation of junin virus (jv) in all susceptible mouse models available induces a lethal meningoencephalitis compatible with a delayed-type hypersensitivity (dth) immune response. in contrast, adult balb/c mice prove resistant to infection and no dth response is seen. jv inoculation in adult balb/c mice induces dth suppression to unrelated antigens such as sheep red blood cells. (srbc). this suppression is mediated by jv-induced t cells (tsv), which are operative from 1 to 24 day ... | 1990 | 1970806 |
| a comparison of junin virus strains: growth characteristics, cytopathogenicity and viral polypeptides. | the growth characteristics, cytopathogenicity and viral polypeptides of the virulent strain xj of junin virus (jv), its attenuated derivative xjc13 and another naturally attenuated jv strain, iv4454, were comparatively studied. iv4454 and xjc13 viruses showed the highest and lowest cytopathology for vero cells, respectively, as measured by plaque morphology, cell viability and inhibition of host cell protein synthesis. the kinetics and electrophoretic patterns of viral polypeptides in infected c ... | 1990 | 2177565 |
| vertical transmission of junin virus in experimentally infected adult calomys musculinus. | the response to infection with junin virus, wild strain cba an 9446, and the antenatal and postnatal transmission of the pathogen in its natural host, calomys musculinus, were studied. intranasal infection in adult animals (90-120 days) did not produce mortality or illness during the 150-day period of observation. from day 21 to 150 after infection, 50% of the animals showed viral persistence with shedding of virus in both urine and saliva. the remaining half became seropositive, and no infectio ... | 1990 | 2177742 |
| vertical transmission of junin virus in experimentally infected adult calomys musculinus. | the response to infection with junin virus, wild strain cba an 9446, and the antenatal and postnatal transmission of the pathogen in its natural host, calomys musculinus, were studied. intranasal infection in adult animals (90-120 days) did not produce mortality or illness during the 150-day period of observation. from day 21 to 150 after infection, 50% of the animals showed viral persistence with shedding of virus in both urine and saliva. the remaining half became seropositive, and no infectio ... | 1990 | 2177742 |
| neurovirulence of wild and laboratory junin virus strains in animal hosts. | the neurovirulence of candid #1 and xjcl3 laboratory strains and cbalv4454 and cbafha5069 wild strains of junin virus was studied in albino mice, guinea pigs, and a south american wild rodent, calomys musculinus, of different ages inoculated by the intracerebral route. infectivity in brain and organs, lethality, and neuropathological lesions were determined. the laboratory and wild strains showed similar neurovirulence only in 2-day-old mice. the neurovirulence of laboratory strains decreased wi ... | 1990 | 2177781 |
| neurovirulence of wild and laboratory junin virus strains in animal hosts. | the neurovirulence of candid #1 and xjcl3 laboratory strains and cbalv4454 and cbafha5069 wild strains of junin virus was studied in albino mice, guinea pigs, and a south american wild rodent, calomys musculinus, of different ages inoculated by the intracerebral route. infectivity in brain and organs, lethality, and neuropathological lesions were determined. the laboratory and wild strains showed similar neurovirulence only in 2-day-old mice. the neurovirulence of laboratory strains decreased wi ... | 1990 | 2177781 |
| heterozygosity and gene flow in populations of calomys laucha (rodentia, cricetidae). | enzyme polymorphism was studied by means of starch gel electrophoresis on five population samples of calomys laucha collected in different sites and seasons. c. laucha, a cricetid rodent inhabiting preferentially cultivated fields in the central-eastern plains of argentina, is a reservoir-host of junin virus, agent of argentine hemorrhagic fever. results show high levels of genic variability in all the samples (p from 62.50 to 77.27; h from 0.118 to 0.163) and low genetic distance among populati ... | 1990 | 2194995 |
| heterozygosity and gene flow in populations of calomys laucha (rodentia, cricetidae). | enzyme polymorphism was studied by means of starch gel electrophoresis on five population samples of calomys laucha collected in different sites and seasons. c. laucha, a cricetid rodent inhabiting preferentially cultivated fields in the central-eastern plains of argentina, is a reservoir-host of junin virus, agent of argentine hemorrhagic fever. results show high levels of genic variability in all the samples (p from 62.50 to 77.27; h from 0.118 to 0.163) and low genetic distance among populati ... | 1990 | 2194995 |
| a mouse attenuated mutant of junin virus with an altered envelope glycoprotein. | the xjc13 strain of junin virus (jv) and the mouse-attenuated mutant c167 showed different gp38 peptide mapping after limited proteolysis with ficin and papain; viral infectivity of both viruses also exhibited a different susceptibility to protease treatment. a correlation between envelope glycoprotein alteration and jv virulence in neonatal mice is proposed. | 1990 | 2162164 |
| astrocytic reaction predominance in chronic encephalitis of junin virus-infected rats. | junin virus antigen distribution and astrocytic reaction to prolonged infection were characterized in rat brain by the pap technique. during the acute stage of neurologic disease following intracerebral inoculation, junin antigen was detected in 100% of animals, strongly in most neurons but also to a much lesser degree in scattered astrocytes, dropping to 20% of rats at 540 days postinfection. initially labeled in all brain areas, viral antigen gradually disappeared from hippocampus but persiste ... | 1989 | 2559956 |
| junin virus monoclonal antibodies: characterization and cross-reactivity with other arenaviruses. | twenty-one monoclonal antibodies reactive with junin virus structural proteins were produced and characterized. using radioimmunoprecipitation and western blot assays, 13 were found to react with the nucleoprotein, seven with the surface glycoprotein and one failed to react, but showed a fluorescent antibody staining pattern consistent with other glycoprotein-specific antibodies. in radioimmunoprecipitation assays, glycoprotein-specific monoclonal antibodies reacted not only with the 35k structu ... | 1989 | 2471803 |
| [rapid serologic diagnosis of argentinian hemorrhagic fever in whole blood]. | the usefulness of a method for detection of antibodies against junin virus in whole blood was tested. n: nih adult mice were inoculated with 10(3) pfu of attenuated xj-clon 3 junin virus strain by intraperitoneal route and blood was obtained by retro-orbital puncture at 21 days post-infection. one blood aliquot (50 microliters) was collected in tubes containing a stabilizer solution for whole blood and another was processed for serum obtention. immunofluorescent antibodies were tested on spot sl ... | 1989 | 2559426 |
| reduced virulence of a junin virus mutant is associated with restricted multiplication in murine cells. | c167, a mutant derived from the xjc13 strain of junin virus, is highly attenuated in its pathogenic properties for newborn mice. whereas 10(2).pfu of xjc13 injected intracerebrally killed 100% of two-day-old mice, the mutant showed no detectable lethality. survival of mice infected with c167 was associated with a reduced and delayed virus replication in brain and a defective spread of virus from the site of inoculation to the other tissues, including spleen, kidney, thymus, liver, peritoneal cel ... | 1989 | 2479190 |
| calomys callidus as a potential junin virus reservoir. | the present study investigated whether c. callidus, a species belonging to the calomys genus, is capable of developing experimentally a persistent junin virus (jv) infection. newborn and adult cricetids were inoculated with the attenuated xj-clone 3 strain of jv by intracerebral or mucosal route. the present results indicate that the species is susceptible to jv infection, capable of shedding virus chronically through saliva and developing a persistent infection as shown by the detection of viru ... | 1989 | 2542433 |
| calomys callidus as a potential junin virus reservoir. | the present study investigated whether c. callidus, a species belonging to the calomys genus, is capable of developing experimentally a persistent junin virus (jv) infection. newborn and adult cricetids were inoculated with the attenuated xj-clone 3 strain of jv by intracerebral or mucosal route. the present results indicate that the species is susceptible to jv infection, capable of shedding virus chronically through saliva and developing a persistent infection as shown by the detection of viru ... | 1989 | 2542433 |
| actions of complement on junin virus. | fresh sera from normal rhesus monkeys, guinea pigs, and rabbits inactivated 90%-99% of the infectivity of vero cell-passaged, attenuated strains of junin virus (jv) within 60 minutes. selective depletion studies showed that inactivation occurred by the classical complement pathway. complement had little effect on virulent jv strains. adsorption of the fresh sera with jv-infected vero cells showed that inactivation was not mediated by low levels of antibodies in normal sera. the cells used for pr ... | 1989 | 2546249 |
| actions of complement on junin virus. | fresh sera from normal rhesus monkeys, guinea pigs, and rabbits inactivated 90%-99% of the infectivity of vero cell-passaged, attenuated strains of junin virus (jv) within 60 minutes. selective depletion studies showed that inactivation occurred by the classical complement pathway. complement had little effect on virulent jv strains. adsorption of the fresh sera with jv-infected vero cells showed that inactivation was not mediated by low levels of antibodies in normal sera. the cells used for pr ... | 1989 | 2546249 |
| nucleocapsid protein gene of junin arenavirus (cdna sequence). | 1989 | 2552421 | |
| protective effect of a low-dose of cyclophosphamide in experimental infection of guinea pigs with junin virus. | administration of cyclophosphamide (cy) to guinea pigs infected with a lethal strain of junin virus (jv) delayed the time of death, with survival of a small number of animals. virological studies showed a temporary decrease of virus concentration in blood and viscera shortly after the cy injection. in the pathological study no differences were found in the organic lesions present in cy-treated and nontreated animals, with the exception of the pulmonary alterations. in cy-treated guinea pigs the ... | 1989 | 2557385 |
| protective effect of a low-dose of cyclophosphamide in experimental infection of guinea pigs with junin virus. | administration of cyclophosphamide (cy) to guinea pigs infected with a lethal strain of junin virus (jv) delayed the time of death, with survival of a small number of animals. virological studies showed a temporary decrease of virus concentration in blood and viscera shortly after the cy injection. in the pathological study no differences were found in the organic lesions present in cy-treated and nontreated animals, with the exception of the pulmonary alterations. in cy-treated guinea pigs the ... | 1989 | 2557385 |
| formalin inactivated junin virus: immunogenicity and protection assays. | the aim of this study was to determine if junin virus inactivated with formalin (fa) was immunogenic and able to elicit a protective response in the guinea pig. the xj-clone 3 strain of junin virus grown in vero cells was exposed to fa at 0 degrees c. the following inactivated antigens were prepared: a1, 0.1% fa for 50 hr; a2, 0.1% fa for 50 hr followed by concentration with polyethylene glycol (peg); b1, 0.05% fa for 70 hr; b2, 0.05% fa for 70 hr plus peg concentration; c, 0.1% fa for 50 hr fol ... | 1989 | 2559158 |
| formalin inactivated junin virus: immunogenicity and protection assays. | the aim of this study was to determine if junin virus inactivated with formalin (fa) was immunogenic and able to elicit a protective response in the guinea pig. the xj-clone 3 strain of junin virus grown in vero cells was exposed to fa at 0 degrees c. the following inactivated antigens were prepared: a1, 0.1% fa for 50 hr; a2, 0.1% fa for 50 hr followed by concentration with polyethylene glycol (peg); b1, 0.05% fa for 70 hr; b2, 0.05% fa for 70 hr plus peg concentration; c, 0.1% fa for 50 hr fol ... | 1989 | 2559158 |
| antigenic relationships between attenuated and pathogenic strains of junin virus. | antigenic relationships between attenuated and pathogenic strains of junin virus (jv) were investigated. five strains of either human or rodent origin were tested by cross-neutralization assay with hyperimmune antisera, raised in rabbits, against each strain. polyclonal antisera could be used to distinguish among these jv strains, as the titer values differed significantly with ratios of homologous to heterologous titers, which ranged from 1.3 to 22.3. this demonstrates, independent of their vir ... | 1989 | 2466103 |
| [protection against encephalitis in rats caused by a pathogenic strain of the junin virus, using peripheral inoculation of an attenuated strain]. | argentine hemorrhagic fever manifests itself in man either subclinically or in hemorrhagic or neurological forms, mortality reaching 20%. although candid 1 strain is undergoing pilot trials, current therapy still resorts to convalescent serum administration. a neurological model was used to evaluate protection conferred by the attenuated xjc13 junin virus strain. newborn rats inoculated intraperitoneally (ip) prove resistant, whereas 8-12 day-old animals infected by intracerebral route with the ... | 1989 | 2562073 |
| ribavirin effect on experimental junin virus-induced encephalitis. | junin virus, the etiological agent of argentine hemorrhagic fever, produces in man a disease mainly characterized by hemorrhagic alterations, commonly accompanied by neurological symptoms, and leading to 10% mortality. intracerebral inoculation in 10-day-old rats or intraperitoneal inoculation in 2-day-old rats leads to high mortality due to severe encephalitis. here, the effect of ribavirin on these experimental models was tested in order to evaluate the degree of protection achieved against ne ... | 1988 | 2846772 |
| ribavirin prophylaxis and therapy for experimental argentine hemorrhagic fever. | junin virus-infected rhesus macaques received prophylactic and therapeutic ribavirin to assess the potential of this drug for treating humans with argentine hemorrhagic fever. when ribavirin was administered intramuscularly at the time of experimental infection with the lethal p3790 strain of junin virus, all animals were protected from clinical disease. a delay in the initiation of therapy until after the onset of illness resulted in improvement and resolution of systemic signs of disease; howe ... | 1988 | 2848441 |
| ribavirin prophylaxis and therapy for experimental argentine hemorrhagic fever. | junin virus-infected rhesus macaques received prophylactic and therapeutic ribavirin to assess the potential of this drug for treating humans with argentine hemorrhagic fever. when ribavirin was administered intramuscularly at the time of experimental infection with the lethal p3790 strain of junin virus, all animals were protected from clinical disease. a delay in the initiation of therapy until after the onset of illness resulted in improvement and resolution of systemic signs of disease; howe ... | 1988 | 2848441 |
| lectin affinity of junin virus glycoproteins. | we studied the binding of junin virus (arenaviridae) glycoproteins, g1 and g2, to two insolubilized lectins. the results showed that mannose, n-acetyl-glucosamine and galactose residues were exposed on g2, while only the latter predominated on g1. heterogeneity of carbohydrate chains was found in g2, the only glycoprotein that was iodinated by the lactoperoxidase method. | 1988 | 2849965 |
| susceptible adult murine model for junin virus. | the adult mouse model had been considered resistant to junin virus (jv) infection. however, we found that c3h/hej murine strain proved highly susceptible up to 5 months of age to intracerebral inoculation with the prototype xj jv strain, showing neurological signs and 80-90% mortality within 13 days. neutralizing antibodies (nt ab) were absent, but low immunofluorescent ab levels (1:5) were detected as from day +7. the virus could only be rescued by coculture of brain samples with vero cells. hi ... | 1988 | 2850346 |
| susceptible adult murine model for junin virus. | the adult mouse model had been considered resistant to junin virus (jv) infection. however, we found that c3h/hej murine strain proved highly susceptible up to 5 months of age to intracerebral inoculation with the prototype xj jv strain, showing neurological signs and 80-90% mortality within 13 days. neutralizing antibodies (nt ab) were absent, but low immunofluorescent ab levels (1:5) were detected as from day +7. the virus could only be rescued by coculture of brain samples with vero cells. hi ... | 1988 | 2850346 |
| long-term protection against argentine hemorrhagic fever in tacaribe virus infected marmosets: virologic and histopathologic findings. | tacaribe virus may represent a better alternative than attenuated strains of junin virus (jv) for immunization against argentine hemorrhagic fever (ahf) because of possible risk of persistent infection of disease associated with live, attenuated strains. callithrix jacchus marmosets, which suffer 100% mortality if inoculated with the pathogenic xj strain of jv, were used to evaluate possible tacaribe virus persistence, subclinical, or long-term disease and the duration of protection against chal ... | 1988 | 2832541 |
| long-term protection against argentine hemorrhagic fever in tacaribe virus infected marmosets: virologic and histopathologic findings. | tacaribe virus may represent a better alternative than attenuated strains of junin virus (jv) for immunization against argentine hemorrhagic fever (ahf) because of possible risk of persistent infection of disease associated with live, attenuated strains. callithrix jacchus marmosets, which suffer 100% mortality if inoculated with the pathogenic xj strain of jv, were used to evaluate possible tacaribe virus persistence, subclinical, or long-term disease and the duration of protection against chal ... | 1988 | 2832541 |
| evaluation of an enzyme-linked immunosorbent assay for quantitation of antibodies to junin virus in human sera. | an enzyme-linked immunosorbent assay (elisa) was evaluated for the quantitation of anti-junin virus (jv) antibodies, in 83 selected cases of argentine haemorrhagic fever (ahf). serum samples were studied in two groups to facilitate comparative analysis; the first group was elisa with indirect immunofluorescence (if) test, in the second elisa with plaque reduction neutralization test (print). from the results obtained by using elisa and if on the same serum samples, a clear tendency of elisa to d ... | 1988 | 2836465 |
| is vertical transmission sufficient to maintain junin virus in nature? | the quantitative contribution of vertical transmission to the prevalence rate of junin virus infection in subsequent generations of its natural reservoir, calomys musculinus, was analysed. data on mortality and reproduction of c. musculinus infected at birth with a wild strain of junin virus were used to estimate the infection-dependent relative survival rate (beta = 0.4849) and relative fertility of the infected host (alpha = 0.2088). prevalence rates of infection, obtained by mathematical simu ... | 1988 | 2838581 |
| is vertical transmission sufficient to maintain junin virus in nature? | the quantitative contribution of vertical transmission to the prevalence rate of junin virus infection in subsequent generations of its natural reservoir, calomys musculinus, was analysed. data on mortality and reproduction of c. musculinus infected at birth with a wild strain of junin virus were used to estimate the infection-dependent relative survival rate (beta = 0.4849) and relative fertility of the infected host (alpha = 0.2088). prevalence rates of infection, obtained by mathematical simu ... | 1988 | 2838581 |
| junin virus-induced delayed-type hypersensitivity suppression in adult mice. | junin virus (jv) infection of suckling mice leads to lethal meningoencephalitis consistent with a delayed-type hypersensitivity (dth)-like immune response. in contrast, there are no central nervous system (cns) alterations, and high antibody titers are induced in resistant adult mice. as a possible explanation, jv infection in adult mice may provoke dth depression. thus in this work we study the alterations induced by jv in the immune response of adult mice by using sheep red blood cells (srbc) ... | 1988 | 2839614 |
| junin virus-induced delayed-type hypersensitivity suppression in adult mice. | junin virus (jv) infection of suckling mice leads to lethal meningoencephalitis consistent with a delayed-type hypersensitivity (dth)-like immune response. in contrast, there are no central nervous system (cns) alterations, and high antibody titers are induced in resistant adult mice. as a possible explanation, jv infection in adult mice may provoke dth depression. thus in this work we study the alterations induced by jv in the immune response of adult mice by using sheep red blood cells (srbc) ... | 1988 | 2839614 |
| epidemiology of argentine hemorrhagic fever. | present knowledge points to horizontal transmission as the most significant mechanism for junín virus maintenance in the main natural reservoirs, namely calomys musculinus and calomys laucha. the existence of naturally infected akodon azarae, both within and outside the endemic area, as well as the finding that other species, ecologically and phylogenetically related to the main reservoirs, such as akodon molinae and calomys callidus, can experimentally develop persistent infections with virus s ... | 1988 | 2841151 |
| epidemiology of argentine hemorrhagic fever. | present knowledge points to horizontal transmission as the most significant mechanism for junín virus maintenance in the main natural reservoirs, namely calomys musculinus and calomys laucha. the existence of naturally infected akodon azarae, both within and outside the endemic area, as well as the finding that other species, ecologically and phylogenetically related to the main reservoirs, such as akodon molinae and calomys callidus, can experimentally develop persistent infections with virus s ... | 1988 | 2841151 |
| differentiation of junin virus and antigenic variants isolated in vivo by kinetic neutralization assays. | the major natural reservoir of junin virus, the aetiological agent of argentine haemorrhagic fever, is the cricetid calomys musculinus. neonatal animals experimentally infected with junin virus (xjcl3 strain) developed typical disease and approximately 80% of them died. most survivors become persistently infected. antigenically variant viruses were isolated from the blood and brain of infected cricetids during the acute and chronic stages of the disease. these variants could be distinguished fro ... | 1988 | 2841418 |
| differentiation of junin virus and antigenic variants isolated in vivo by kinetic neutralization assays. | the major natural reservoir of junin virus, the aetiological agent of argentine haemorrhagic fever, is the cricetid calomys musculinus. neonatal animals experimentally infected with junin virus (xjcl3 strain) developed typical disease and approximately 80% of them died. most survivors become persistently infected. antigenically variant viruses were isolated from the blood and brain of infected cricetids during the acute and chronic stages of the disease. these variants could be distinguished fro ... | 1988 | 2841418 |
| mortality induced by adoptive immunity in junin virus-infected athymic mice. | the effect of normal or sensitized spleen cell transfer from syngeneic euthymic mice to junin virus-infected suckling athymic mice was studied. transfer was performed 1 or 7 days after infection. in both cases, an acute lethal disease developed 6-11 days after transfer. the mortality reached 100% in all infected groups receiving normal or sensitized splenocytes, while it was negligible for different control groups of athymic mice. transfer of normal or sensitized splenocytes was unable to signif ... | 1988 | 2842272 |
| in vivo junin virus-mouse macrophages interaction. | the role of mononuclear phagocytic cells in extraneural infection of the mouse with junin virus (jv) was studied. endpoint susceptibility (4 days of life) was evaluated by intraperitoneal (i.p.) inoculation of suckling mice. by means of immunofluorescence (if) and c3 receptor assays, it was found that macrophages were permissive to viral replication in vivo and fostered the recruitment of inflammatory cells as evidenced by the absence of c3 marker. in support, in vitro infection failed to induce ... | 1988 | 2902768 |
| circulating interferon in the guinea pig infected with the xj, prototype junin virus strain. | the interferon (ifn) induction capacity of the xj prototype strain of junín virus (jv) was investigated in the guinea pig model. circulating alpha ifn was detected in 50% of the animals from days 2 to 9 postinfection (pi) and in 100% at day 11 pi, when all animals were in the premortem stage. individual levels ranged from 20 to 1,280 guinea pig ifn units (gpifnu)/ml. a correlation between xj strain virulence and ifn titers was recorded. a possible role of ifn as a pathogenic factor in the outcom ... | 1988 | 2828536 |
| circulating interferon in the guinea pig infected with the xj, prototype junin virus strain. | the interferon (ifn) induction capacity of the xj prototype strain of junín virus (jv) was investigated in the guinea pig model. circulating alpha ifn was detected in 50% of the animals from days 2 to 9 postinfection (pi) and in 100% at day 11 pi, when all animals were in the premortem stage. individual levels ranged from 20 to 1,280 guinea pig ifn units (gpifnu)/ml. a correlation between xj strain virulence and ifn titers was recorded. a possible role of ifn as a pathogenic factor in the outcom ... | 1988 | 2828536 |
| viral strain dependent differences in experimental argentine hemorrhagic fever (junin virus) infection of guinea pigs. | guinea pigs infected with low-passage junin virus of human origin showed viral strain dependent differences in mortality, ld50, time to death, and in viral spread and distribution. different junin strains appeared to cause at least two broad patterns of argentine hemorrhagic fever in guinea pigs. a number of strains of junin virus caused a viscerotropic type of illness in which virus replicated predominantly in lymph nodes, spleen, and bone marrow. with the most severe visceral forms of argentin ... | 1988 | 2846464 |
| viral strain dependent differences in experimental argentine hemorrhagic fever (junin virus) infection of guinea pigs. | guinea pigs infected with low-passage junin virus of human origin showed viral strain dependent differences in mortality, ld50, time to death, and in viral spread and distribution. different junin strains appeared to cause at least two broad patterns of argentine hemorrhagic fever in guinea pigs. a number of strains of junin virus caused a viscerotropic type of illness in which virus replicated predominantly in lymph nodes, spleen, and bone marrow. with the most severe visceral forms of argentin ... | 1988 | 2846464 |
| suppressor t-cell population induced by junin virus in adult mice. | intracerebral (i.c.) junin virus (jv) infection of adult balb/c mice is characterized by the absence of morbidity and a low mortality (barely 8-10%). in contrast, the suckling mouse model exhibits almost 100% mortality following central nervous system (cns) alterations consistent with a delayed-type hypersensitivity (dth)-like immune response. besides, jv infection of adult (resistant) mice leads to immunosuppression of dth to unrelated antigens. here we present evidence demonstrating that such ... | 1988 | 2970429 |
| mouse splenocyte transfer effect depends on donor's junin virus infection stage. | splenocytes from junin-virus-persistently-infected euthymic mice taken at 45 days postinfection seemed unable to induce overt signs of disease, to cause death, or to modify brain viral levels when transferred to athymic junin-virus-infected mice. findings differed sharply when the same recipients were transferred with splenocytes taken at 6 or 30 days postinfection from immunocompetent mice infected in adult life, since mortality reached 80 or 50%, respectively, and brain viral titers were signi ... | 1988 | 3260227 |
| experimental argentine hemorrhagic fever in rhesus macaques: virus-specific variations in pathology. | two isolates of junin virus (espindola and ledesma) inoculated into rhesus macaques produced distinct lesions which were strain-constant and similar to reported human cases of argentine hemorrhagic fever. the espindola isolate was associated with hemorrhagia, necrosis of bone marrow, and mild hepatocellular necrosis. ledesma isolate was associated with pronounced polioencephalomyelitis and autonomic ganglioneuritis, but very mild or absent hepatocellular necrosis, bone marrow necrosis, and hemor ... | 1987 | 3039051 |
| experimental argentine hemorrhagic fever in rhesus macaques: virus-specific variations in pathology. | two isolates of junin virus (espindola and ledesma) inoculated into rhesus macaques produced distinct lesions which were strain-constant and similar to reported human cases of argentine hemorrhagic fever. the espindola isolate was associated with hemorrhagia, necrosis of bone marrow, and mild hepatocellular necrosis. ledesma isolate was associated with pronounced polioencephalomyelitis and autonomic ganglioneuritis, but very mild or absent hepatocellular necrosis, bone marrow necrosis, and hemor ... | 1987 | 3039051 |
| intracerebral infection of cebus apella with the xj-clone 3 strain of junín virus. | to assess the usefulness of the south american primate cebus apella as a model for neurovirulence of junín virus, eight monkeys were inoculated with 10(5) ld50 of the attenuated xj-clone 3 junín virus strain by the intrathalamic route. after the second week, weight loss and polyadenopathies were observed in most animals, one-half of which had a transient leukothrombocytopenia. moderate clinical central nervous system (cns) involvement was present in four of eight monkeys, while the rest had only ... | 1987 | 3031201 |
| intracerebral infection of cebus apella with the xj-clone 3 strain of junín virus. | to assess the usefulness of the south american primate cebus apella as a model for neurovirulence of junín virus, eight monkeys were inoculated with 10(5) ld50 of the attenuated xj-clone 3 junín virus strain by the intrathalamic route. after the second week, weight loss and polyadenopathies were observed in most animals, one-half of which had a transient leukothrombocytopenia. moderate clinical central nervous system (cns) involvement was present in four of eight monkeys, while the rest had only ... | 1987 | 3031201 |
| effect of polymorphonuclear depletion on experimental argentine hemorrhagic fever in guinea pigs. | the role that polymorphonuclear leukocytes (pmn) may play in argentine hemorrhagic fever (ahf), an endemo-epidemic disease caused by junín virus (jv), was investigated in experimentally infected guinea pigs depleted of pmn by means of specific antiserum. in leucopenic animals the evolution of the infection with a highly pathogenic strain of jv was more severe, with earlier mortality and higher virus yields in blood and viscera. the pathological study showed similar lesions in both the control an ... | 1987 | 3040897 |
| effect of polymorphonuclear depletion on experimental argentine hemorrhagic fever in guinea pigs. | the role that polymorphonuclear leukocytes (pmn) may play in argentine hemorrhagic fever (ahf), an endemo-epidemic disease caused by junín virus (jv), was investigated in experimentally infected guinea pigs depleted of pmn by means of specific antiserum. in leucopenic animals the evolution of the infection with a highly pathogenic strain of jv was more severe, with earlier mortality and higher virus yields in blood and viscera. the pathological study showed similar lesions in both the control an ... | 1987 | 3040897 |
| protection of junín virus-infected marmosets by passive administration of immune serum: association with late neurologic signs. | argentine hemorrhagic fever (junín virus) is a human viral disease for which immune therapy proves effective, though a late neurologic syndrome is occasionally associated with the treatment. we attempted to determine in the infected marmoset callithrix jacchus whether immune therapy leads to protection and/or cns damage. fifteen c jacchus were inoculated with 10(3) tissue culture infectious dose 50% (tcid50) of the xj strain of junín virus. on day 6 post infection (pi), 12 primates were treated ... | 1987 | 3025358 |
| protection of junín virus-infected marmosets by passive administration of immune serum: association with late neurologic signs. | argentine hemorrhagic fever (junín virus) is a human viral disease for which immune therapy proves effective, though a late neurologic syndrome is occasionally associated with the treatment. we attempted to determine in the infected marmoset callithrix jacchus whether immune therapy leads to protection and/or cns damage. fifteen c jacchus were inoculated with 10(3) tissue culture infectious dose 50% (tcid50) of the xj strain of junín virus. on day 6 post infection (pi), 12 primates were treated ... | 1987 | 3025358 |
| [heterogeneity in the virulence of viral subpopulations derived from an attenuated strain of junin virus]. | 1987 | 2854292 | |
| junín virus persistence in mice. | newborn mice surviving intracerebral infection with junín virus (jv) strain xj showed viral persistence in brain up to 140 days post-infection (p.i.). mild meningoencephalitis or encephalitis, but not the neutralizing antibody titres (ntab) correlated with virus presence. | 1987 | 2883864 |
| abrogation of junin virus encephalitis by critical cyclophosphamide timing and dosage. | junin virus-induced encephalitis in suckling mouse is a delayed-type hypersensitivity reaction, whose immunopathologic nature has been proven by suppressing the thymus-dependent response. cyclophosphamide (cy) given at day +6 post-infection (p.i.) has been shown to modulate infection, presumably by tdth lymphocyte inactivation. to determine critical timing and i.p. drug dose, brain histology and survival were studied in 3-day-old balb/c mice, inoculated i.c. with junin virus. optimal protection ... | 1987 | 2888291 |
| macrophages are involved in age-dependent resistance of rats to junin virus infection. | we attempted to correlate rat age with resistance to intraperitoneal infection with the xj strain of junin virus. accordingly, mortality, viral replication in macrophages and brain, as well as neutralizing antibody (na) levels were recorded in animals inoculated at 2, 5, 10 and 26 days of life. two-day-old animals demonstrated both the greatest mortality (86%) and viral replication in macrophages, allowing virus to reach the brain where high titers were detected. this age group also had the high ... | 1987 | 2822597 |
| interferon response in the guinea pig infected with junín virus. | the "in vivo" interferon (ifn) induction capacity of two junín virus strains--the attenuated xjcl3 and the intermediate virulent mc2--was studied in the guinea pig experimental model. three different doses of xjcl3 strain--2,000, 10,000, and 50,000 tcid50--and a single dose of 10,000 tcid50 of mc2 were assayed. animals were bled from day 0 to day 14 postinjection (pi) xjcl3 groups showed a constant serum ifn response. mc2 infection showed that 16% of the animals failed to develop interferonemia. ... | 1987 | 2445908 |
| interferon response in the guinea pig infected with junín virus. | the "in vivo" interferon (ifn) induction capacity of two junín virus strains--the attenuated xjcl3 and the intermediate virulent mc2--was studied in the guinea pig experimental model. three different doses of xjcl3 strain--2,000, 10,000, and 50,000 tcid50--and a single dose of 10,000 tcid50 of mc2 were assayed. animals were bled from day 0 to day 14 postinjection (pi) xjcl3 groups showed a constant serum ifn response. mc2 infection showed that 16% of the animals failed to develop interferonemia. ... | 1987 | 2445908 |
| [attenuation in the virulence of a mutant of junin virus in suckling mice]. | the virulence in neonatal mice of a temperature-sensitive mutant of junin virus, named c167, was studied. the thermosensitive properties of this mutant were tested by titration on vero cells at 37 and 40 degrees c. the ratio of infectivity 40/37 was approximately 100-fold lower for c167 with respect to xjc13 (table 1). the attenuation of c167 was determined by measurement of mean survival time and 50% lethal dose after intracerebral injection of 2 and 11 day old mice. for c167 the lethality inde ... | 1987 | 2825245 |
| effect of persistent infection with junin virus on growth and reproduction of its natural reservoir, calomys musculinus. | the effect of infection with junin virus on growth and reproduction of its natural reservoir, calomys musculinus, was studied. eighty-five c. musculinus were inoculated intranasally at birth with 100 tcid50 of cba an 9446 strain of junin virus and observed for 480 days. no clinical signs of neurologic illness were registered. infected animals showed an increased mortality rate of up to 70% between days 24-40 post-infection. this period of high mortality was preceded by low weight gain during lac ... | 1987 | 2825553 |
| effect of persistent infection with junin virus on growth and reproduction of its natural reservoir, calomys musculinus. | the effect of infection with junin virus on growth and reproduction of its natural reservoir, calomys musculinus, was studied. eighty-five c. musculinus were inoculated intranasally at birth with 100 tcid50 of cba an 9446 strain of junin virus and observed for 480 days. no clinical signs of neurologic illness were registered. infected animals showed an increased mortality rate of up to 70% between days 24-40 post-infection. this period of high mortality was preceded by low weight gain during lac ... | 1987 | 2825553 |
| tacaribe virus: a new alternative for argentine hemorrhagic fever vaccine. | tacaribe virus is know to protect guinea pigs and primates against lethal challenge with junín virus. a long-term study on the effect of tacaribe virus infection in the guinea pig was carried out to determine the extent of cross-protection and whether antigen and/or viral persistence and tissue damage could be detected in immune animals. viral titers, antigen expression in organs, and histologic lesions were sequentially searched for up to 540 days postinfection (pi). neutralizing antibodies (ab ... | 1987 | 2828522 |
| tacaribe virus: a new alternative for argentine hemorrhagic fever vaccine. | tacaribe virus is know to protect guinea pigs and primates against lethal challenge with junín virus. a long-term study on the effect of tacaribe virus infection in the guinea pig was carried out to determine the extent of cross-protection and whether antigen and/or viral persistence and tissue damage could be detected in immune animals. viral titers, antigen expression in organs, and histologic lesions were sequentially searched for up to 540 days postinfection (pi). neutralizing antibodies (ab ... | 1987 | 2828522 |
| [protection conferred by a hyperimmune serum and its fractions on rats infected with the junin virus]. | suckling rats infected by ic route with 10(3)ld50 of the xjc13 strain of jv were passively immunized with homologous hyperimmune serum (his). animals treated at 2 days pi with his showed a significant increase in survival vs. non-treated infected controls (82% vs 5%). however, at 4 days pi, transfer failed to modify survival. by means of deae sephadex a25 column chromatography, the presence of neutralizing immunoglobulin closely correlated with protective antibodies, but were not restricted to t ... | 1986 | 2825240 |
| antiviral effect of ribavirin on junin virus replication in vitro. | the effect of ribavirin (1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide) on the replication in vitro of junin virus, the causative agent of argentine hemorrhagic fever (ahf), was examined. a concentration as low as 3.12 micrograms/ml of the drug inhibited the cytopathic effect observed five days post-infection (pi) on vero cells. on the other hand, a concentration of 25 micrograms/ml reduced the virus yield and specific antigen formation to undetectable levels. this same concentration of ri ... | 1986 | 2825242 |
| cytolysis of junin infected target cells by immune guinea pig spleen cells. | spleen cells from guinea pigs infected with an attenuated strain of junin virus (the causative agent of argentine hemorrhagic fever) specifically lysed virus-infected syngeneic target cells in vitro. this activity was detected as early as 6 days after infection, reached a maximum on days 10-13, and persisted at lower levels, at least through day 30. monoclonal antibody to guinea pig t cells had no effect on the activity. after b or t cell enrichment techniques, the cytolysis was found with the b ... | 1986 | 2854602 |
| cytolysis of junin infected target cells by immune guinea pig spleen cells. | spleen cells from guinea pigs infected with an attenuated strain of junin virus (the causative agent of argentine hemorrhagic fever) specifically lysed virus-infected syngeneic target cells in vitro. this activity was detected as early as 6 days after infection, reached a maximum on days 10-13, and persisted at lower levels, at least through day 30. monoclonal antibody to guinea pig t cells had no effect on the activity. after b or t cell enrichment techniques, the cytolysis was found with the b ... | 1986 | 2854602 |
| experimental argentine hemorrhagic fever: myocardial involvement in cebus monkey. | four cebus apella monkeys, to test a recently proposed model for testing neurovirulence of junín virus (jv) strains, were intracerebrally infected with 10(5) ld50 of the xj clone 3 strain of jv. there were no significant electrocardiographic abnormalities or gross lesions, but all infected monkeys exhibited a varying degree of histologic myocardial lesions including focal lymphoblastic infiltrates, vascular ruptures, and mild interstitial reactive change. one cebus showed lymphocytic infiltrates ... | 1986 | 3025446 |
| experimental argentine hemorrhagic fever: myocardial involvement in cebus monkey. | four cebus apella monkeys, to test a recently proposed model for testing neurovirulence of junín virus (jv) strains, were intracerebrally infected with 10(5) ld50 of the xj clone 3 strain of jv. there were no significant electrocardiographic abnormalities or gross lesions, but all infected monkeys exhibited a varying degree of histologic myocardial lesions including focal lymphoblastic infiltrates, vascular ruptures, and mild interstitial reactive change. one cebus showed lymphocytic infiltrates ... | 1986 | 3025446 |
| effect of ribavirin and tributylribavirin on argentine hemorrhagic fever (junin virus) in guinea pigs. | subcutaneous injections of ribavirin into guinea pigs infected intraperitoneally or intracerebrally with junin virus significantly increased the mean time to death but did not enhance survival of the animals. we found similar results with tributylribavirin. virus replication was delayed, but not prevented, in ribavirin-treated infected guinea pigs. the animals usually died with high virus titers in their brains and frequently were paralyzed. | 1986 | 3013087 |
| effect of ribavirin and tributylribavirin on argentine hemorrhagic fever (junin virus) in guinea pigs. | subcutaneous injections of ribavirin into guinea pigs infected intraperitoneally or intracerebrally with junin virus significantly increased the mean time to death but did not enhance survival of the animals. we found similar results with tributylribavirin. virus replication was delayed, but not prevented, in ribavirin-treated infected guinea pigs. the animals usually died with high virus titers in their brains and frequently were paralyzed. | 1986 | 3013087 |