Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| a case of maternal herpes simplex virus encephalitis during late pregnancy. | a pregnant 25-year-old woman at 32 weeks' gestation was admitted to an emergency unit after her husband had found her drowsy and with her tongue bitten. the day before admission, the patient had developed a fever of 39 degrees c, was suffering from headaches, was nauseated and had vomited. on admission, she had anterograde and retrograde amnesia, but no somatic neurological deficits were detected. | 2009 | 19129790 |
| cell cycle inhibitor enhances the resolution of hsv-1-induced proinflammatory response in murine microglial cells. | herpes simplex encephalitis remains one of the most devastating intracranial infections despite available antiviral treatment, with sequelae secondary to a persistent inflammatory response. recently, cyclin-dependent kinases (cdks) have been found to act as cellular targets for antiviral drugs. however, the pharmacological effects of cdk inhibitors on glial cell function in herpes simplex encephalitis have not been elucidated. the aim of this work was to determine the influence of olomoucine on ... | 2009 | 19138471 |
| the bipolar filaments formed by herpes simplex virus type 1 ssb/recombination protein (icp8) suggest a mechanism for dna annealing. | herpes simplex virus type 1 encodes a multifunctional protein, icp8, which serves both as a single-strand binding protein and as a recombinase, catalyzing reactions involved in replication and recombination of the viral genome. in the presence of divalent ions and at low temperature, previous electron microscopic studies showed that icp8 will form long left-handed helical filaments. here, electron microscopic image reconstruction reveals that the filaments are bipolar, with an asymmetric unit co ... | 2009 | 19138689 |
| erythema nodosum-like lesions in treated whipple's disease: signs of immune reconstitution inflammatory syndrome. | treatment of systemic infections due to mycobacteria and hiv infection can lead to paradoxical worsening, the immune reconstitution inflammatory syndrome, in a minority of patients. herein we describe a patient with whipple's disease, a chronic systemic inflammatory disease caused by tropheryma whipplei, who developed cutaneous and later ocular disease after initiation of antibiotic therapy. a 42-year-old man with a 12-year history of arthralgias presented with deteriorating health, including we ... | 2009 | 19150271 |
| herpesvirus gb-induced fusion between the virion envelope and outer nuclear membrane during virus egress is regulated by the viral us3 kinase. | herpesvirus capsids collect along the inner surface of the nuclear envelope and bud into the perinuclear space. enveloped virions then fuse with the outer nuclear membrane (nm). we previously showed that herpes simplex virus (hsv) glycoproteins gb and gh act in a redundant fashion to promote fusion between the virion envelope and the outer nm. hsv mutants lacking both gb and gh accumulate enveloped virions in herniations, vesicles that bulge into the nucleoplasm. earlier studies had shown that h ... | 2009 | 19158241 |
| herpes simplex virus utilizes the large secretory vesicle pathway for anterograde transport of tegument and envelope proteins and for viral exocytosis from growth cones of human fetal axons. | axonal transport of herpes simplex virus (hsv-1) is essential for viral infection and spread in the peripheral nervous system of the host. therefore, the virus probably utilizes existing active transport and targeting mechanisms in neurons for virus assembly and spread from neurons to skin. in the present study, we used transmission immunoelectron microscopy to investigate the nature and origin of vesicles involved in the anterograde axonal transport of hsv-1 tegument and envelope proteins and o ... | 2009 | 19176621 |
| gsk983: a novel compound with broad-spectrum antiviral activity. | gsk983, a novel tetrahydrocarbazole, inhibits the replication of a variety of unrelated viruses in vitro with ec(50) values of 5-20 nm. both replication of the adenovirus ad-5 and the polyoma virus sv-40, and episomal maintenance of human papillomaviruses (hpv) and epstein-barr virus (ebv) are susceptible to gsk983. the compound does not inhibit all viruses; herpes simplex virus (hsv-1), human immunodeficiency virus (hiv), and lytic replication of ebv were not susceptible at concentrations below ... | 2009 | 19187793 |
| influence of antisense oligonucleotides targeting tumor necrosis factor-alpha on experimental herpetic-induced chorioretinitis of mouse eye. | to investigate the effect on the pathological process of topical treatment with antisense oligonucleotides (ason) targeting tumor necrosis factor-alpha (tnf-alpha) in experimental herpes simplex virus type-1 (hsv-1)-induced chorioretinitis of mouse eye. | 2009 | 19188750 |
| [bell's palsy: from viral aetiology to diagnostic reality]. | peripheral injury of the facial nerve is a frequent disorder. it is a stressful situation for the patient and it is functionally hazardous for the cornea. facial palsy is due to a lesion involving the facial pontine nucleus or the nerve trunk in its route from the pontocerebellar angle to the parotid. the idiopathic facial paralysis or bell's palsy (bp) is the most common cause but acute facial palsy can also be due to tumors. a rigorous clinical history and examination must be performed to guid ... | 2009 | 19195745 |
| effects of herpes simplex virus amplicon transduction on murine dendritic cells. | the herpes simplex virus (hsv)-based amplicon is a versatile vaccine platform that has been preclinically vetted as a gene-based immunotherapeutic for cancer, hiv, and neurodegenerative disorders. although it is well known that injection of dendritic cells (dcs) transduced ex vivo with helper virus-free hsv amplicon vectors expressing disease-relevant antigens induces antigen-specific immune responses, the cellular receptor(s) by which the amplicon virion gains entry into dcs, as well as the eff ... | 2009 | 19199821 |
| fewer latent herpes simplex virus type 1 and cytotoxic t cells occur in the ophthalmic division than in the maxillary and mandibular divisions of the human trigeminal ganglion and nerve. | following primary infection of the mouth, herpes simplex virus type 1 (hsv-1) travels retrogradely along the maxillary (v2) or mandibular (v3) nerve to the trigeminal ganglion (tg), where it establishes lifelong latency. symptomatic hsv-1 reactivations frequently manifest as herpes labialis, while ocular hsv-1 disease is rare. we investigated whether these clinical observations are mirrored by the distribution of latent hsv-1 as well as cytotoxic t-cell infiltration around the nerve cell bodies ... | 2009 | 19211753 |
| alzheimer's disease-specific tau phosphorylation is induced by herpes simplex virus type 1. | neurofibrillary tangles are one of the main neuropathological features of alzheimer's disease (ad) and are composed of abnormally phosphorylated forms of a microtubule-associated protein called tau. what causes this abnormal phosphorylation is unknown. our previous studies have implicated herpes simplex virus type 1 (hsv1) as an etiological agent in ad, and so we investigated whether infection with this virus induces ad-like tau phosphorylation. here we demonstrate that hsv1 causes tau phosphory ... | 2009 | 19221424 |
| in vitro antioxidant, antimicrobial and anti-herpes simplex virus type 1 activity of phellodendron amurense rupr. from china. | phellodendron amurense rupr. bark extracts were examined for antioxidant activity, antimicrobial activity and antiviral activity on herpes simplex virus type 1 (hsv-1). ethanol extract showed higher content of both total phenolic and flavonoid than aqueous extract. in dpph (2, 2-diphenyl-1-picrylhydrazyl) assay, the concentration providing 50% inhibition (ic(50)) values were 6.73 +/- 0.87 mg/ml and 4.26 +/- 0.59 mg/ml for aqueous and ethanol extracts respectively. ethanol extract had a much high ... | 2009 | 19222122 |
| molecular-genetic pet imaging using an hsv1-tk mutant reporter gene with enhanced specificity to acycloguanosine nucleoside analogs. | imaging 2 different molecular-genetic events in a single subject by pet is essential in a variety of in vivo applications. using herpes simplex virus-1 thymidine kinase (hsv1-tk) mutants with narrower substrate specificities in combination with wild-type hsv1-tk (wthsv1-tk) would enable differential imaging with corresponding radiotracers, namely 2'-deoxy-2'-(18)f-fluoro-5-ethyl-1-beta-d-arabinofuranosyl-uracil ((18)f-feau) and the acycloguanosine derivative 9-(4-(18)f-fluoro-3-[hydroxymethyl]bu ... | 2009 | 19223410 |
| viral supraglottitis in an adult. | 2009 | 19224474 | |
| detection of herpes viruses in children with acute appendicitis. | this study aimed to investigate the incidence of herpes simplex virus (hsv) types-1 and -2, varicella-zoster virus (vzv), cytomegalovirus (cmv), epstein-barr virus (ebv), human herpes virus 6 (hhv-6) and human herpes virus 7 (hhv-7) in childhood acute appendicitis. | 2009 | 19233720 |
| entry of herpes simplex virus 1 and other alphaherpesviruses via the paired immunoglobulin-like type 2 receptor alpha. | herpes simplex virus 1 (hsv-1) enters cells either via fusion of the virion envelope and host cell plasma membrane or via endocytosis, depending on the cell type. in the study reported here, we investigated a viral entry pathway dependent on the paired immunoglobulin-like type 2 receptor alpha (pilralpha), a recently identified entry coreceptor for hsv-1 that associates with viral envelope glycoprotein b (gb). experiments using inhibitors of endocytic pathways and ultrastructural analyses of chi ... | 2009 | 19244335 |
| [fullevir: in vitro and in vivo antiherpetic activity]. | the antiherpetic properties of a fullerene derivative with aminocaproic acid (manufactured by intelfarm co. as fullevir) were studied in in vitro (in sensitive cell cultures) and in vivo (on a murine model of experimental herpetic encephalitis) experiments. fullevir was found to protect tissue culture cells from the cytodestructive action of herpes simplex virus type 1. it was estimated that ed50 = 5.3 microg/ml and ed90 = 29.1 microg/ml. the agent was most effective when it was administered bef ... | 2009 | 19253725 |
| the epstein-barr virus alkaline exonuclease bglf5 serves pleiotropic functions in virus replication. | the epstein-barr virus (ebv) alkaline exonuclease bglf5 has previously been recognized to contribute to immune evasion by downregulating production of hla molecules during virus replication. we have constructed a bglf5-null virus mutant to determine bglf5's functions during ebv viral replication. quantification of virus production in permissive 293 cells carrying a deltabglf5 genome identified a 17- to 21-fold reduction relative to complemented or wild-type controls. detailed monitoring of delta ... | 2009 | 19264771 |
| herpes simplex virus type 1 regulatory protein icp0 aids infection in cells with a preinduced interferon response but does not impede interferon-induced gene induction. | several independent lines of evidence indicate that interferon-mediated innate responses are involved in controlling herpes simplex virus type 1 (hsv-1) infection and that the viral immediate-early regulatory protein icp0 augments hsv-1 replication in interferon-treated cells. however, this is a complex situation in which the experimental outcome is determined by the choice of multiplicity of infection and cell type and by whether cultured cells or animal models are used. it is now known that ne ... | 2009 | 19264774 |
| [update on the treatment of genital herpes]. | genital herpes is a chronic infection characterized by periodic reactivation. it can produce symptomatic disease in the host although asymptomatic viral excretion can also occur. it is currently the main cause of genital ulceration and an important public health problem that has substantial clinical, psychological, and economic repercussions. this review analyzes the currently available therapeutic options and regimens, which are based mainly on systemic use of antiviral agents such as aciclovir ... | 2009 | 19268108 |
| herpes simplex virus type 1 glycoprotein e mediates retrograde spread from epithelial cells to neurites. | in animal models of infection, glycoprotein e (ge) is required for efficient herpes simplex virus type 1 (hsv-1) spread from the inoculation site to the cell bodies of innervating neurons (retrograde direction). retrograde spread in vivo is a multistep process, in that hsv-1 first spreads between epithelial cells at the inoculation site, then infects neurites, and finally travels by retrograde axonal transport to the neuron cell body. to better understand the role of ge in retrograde spread, we ... | 2009 | 19279108 |
| virion incorporation of the herpes simplex virus type 1 tegument protein vp22 occurs via glycoprotein e-specific recruitment to the late secretory pathway. | the mechanism by which herpesviruses acquire their tegument is not yet clear. one model is that outer tegument proteins are recruited by the cytoplasmic tails of viral glycoproteins. in the case of herpes simplex virus tegument protein vp22, interactions with the glycoproteins ge and gd have been shown. we have previously shown that the c-terminal half of vp22 contains the necessary signal for assembly into the virus. here, we show that during infection vp22 interacts with ge and gm, as well as ... | 2009 | 19279114 |
| isolation and preliminary characterization of herpes simplex virus 1 primary enveloped virions from the perinuclear space. | herpes simplex virus 1 (hsv-1) nucleocapsids exit the nucleus by budding into the inner nuclear membrane, where they exist briefly as primary enveloped virions. these virus particles subsequently fuse their envelopes with the outer nuclear membrane, permitting nucleocapsids to then enter the cytoplasm and complete assembly. we have developed a method to isolate primary enveloped virions from hsv-1-infected cells and subjected the primary enveloped virion preparation to maldi-ms/ms (matrix-assist ... | 2009 | 19279117 |
| kinetics of primary and memory cytotoxic t lymphocyte responses to herpes simplex virus 1 infection: granzyme b mediated ctl activity. | herpes simplex virus type 1 is one of the most common viruses among human population. studies demonstrate the essential role of cell mediated immunity, especially cd8+ t cells, in prevention and clearance of hsv1. | 2009 | 19293474 |
| extreme susceptibility of african naked mole rats (heterocephalus glaber) to experimental infection with herpes simplex virus type 1. | herpes simplex virus type 1 (hsv1) is widely used as a gene delivery vector in a variety of laboratory animals. in a recent study, a thymidine-kinase-inactive (replication-conditional) hsv1 used as a delivery vector was lethal in naked mole rats, whereas mice infected with the identical virus showed no adverse effects. this result prompted us to undertake a controlled comparative histologic study of the effect of hsv1 infection on naked mole rats and mice. replication-competent and replication-c ... | 2009 | 19295058 |
| phenotypic similarities and differences between ul37-deleted pseudorabies virus and herpes simplex virus type 1. | in the absence of the tegument protein pul37, virion formation of pseudorabies virus (prv) and herpes simplex virus type 1 (hsv-1) is severely impaired. non-enveloped nucleocapsids accumulate in clusters in the cytoplasm, whereas only a few enveloped particles can be detected. although a contribution of pul37 to nuclear egress of hsv-1 has been suggested, the nuclear stages of morphogenesis are not impaired in prv-deltaul37-infected cells. moreover, hsv-1 pul37 has been described as essential fo ... | 2009 | 19297610 |
| characterization of an immediate splenic precursor of cd8+ dendritic cells capable of inducing antiviral t cell responses. | mouse spleens contain three major dendritic cell (dc) populations: plasmacytoid dc, conventional cd8(+)cd24(+) dc (cd8(+) dc), and conventional cd8(-)cd24(-) dc (cd8(-) dc). we have previously shown that cd8(+) dc are the major cross-presenting subtype in vivo and are the main inducers of antiviral cytotoxic t lymphocyte responses. here we show that after depletion of cd8(+) dc, the only dc capable of viral ag presentation was a small subset that expresses cd24 but not cd8. this cd8(-)cd24(+) dc ... | 2009 | 19299718 |
| identification of multiple sites suitable for insertion of foreign genes in herpes simplex virus genomes. | information on sites in hsv genomes at which foreign gene(s) can be inserted without disrupting viral genes or affecting properties of the parental virus are important for basic research on hsv and development of hsv-based vectors for human therapy. the intergenic region between hsv-1 ul3 and ul4 genes has been reported to satisfy the requirements for such an insertion site. the ul3 and ul4 genes are oriented toward the intergenic region and, therefore, insertion of a foreign gene(s) into the re ... | 2009 | 19302526 |
| autophagy enhances the presentation of endogenous viral antigens on mhc class i molecules during hsv-1 infection. | viral proteins are usually processed by the 'classical' major histocompatibility complex (mhc) class i presentation pathway. here we showed that although macrophages infected with herpes simplex virus type 1 (hsv-1) initially stimulated cd8(+) t cells by this pathway, a second pathway involving a vacuolar compartment was triggered later during infection. morphological and functional analyses indicated that distinct forms of autophagy facilitated the presentation of hsv-1 antigens on mhc class i ... | 2009 | 19305394 |
| arginine methylation of the icp27 rgg box regulates icp27 export and is required for efficient herpes simplex virus 1 replication. | the herpes simplex virus 1 (hsv-1) multifunctional regulatory protein icp27 shuttles between the nucleus and cytoplasm in its role as a viral mrna export factor. arginine methylation on glycine- and arginine-rich motifs has been shown to regulate protein export. icp27 contains an rgg box and has been shown to be methylated during viral infection. we found by mass spectrometric analysis that three arginine residues within the rgg box were methylated. viral mutants with substitutions of lysine for ... | 2009 | 19321610 |
| regulation of histone deposition on the herpes simplex virus type 1 genome during lytic infection. | during lytic infection by herpes simplex virus type 1 (hsv-1), histones are present at relatively low levels on the viral genome. however, the mechanisms that account for such low levels--how histone deposition on the viral genome is blocked or how histones are removed from the genome--are not yet defined. in this study, we show that histone occupancy on the viral genome gradually increased with time when transcription of the viral immediate-early (ie) genes was inhibited either by deletion of t ... | 2009 | 19321615 |
| towards fibroid gene therapy: adenovirus-mediated delivery of herpes simplex virus 1 thymidine kinase gene/ganciclovir shrinks uterine leiomyoma in the eker rat model. | the objective of this study was to assess in vivo gene therapy of uterine leiomyomas in the eker rat model using adenovirus (ad)-mediated delivery of herpes simplex virus 1 thymidine kinase gene (hsv1tk) followed by ganciclovir (gcv) treatment. | 2009 | 19325244 |
| role of chromatin during herpesvirus infections. | dna viruses have long served as model systems to elucidate various aspects of eukaryotic gene regulation, due to their ease of manipulation and relatively low complexity of their genomes. in some cases, these viruses have revealed mechanisms that are subsequently recognized to apply also to cellular genes. in other cases, viruses adopt mechanisms that prove to be exceptions to the more general rules. the double-stranded dna viruses that replicate in the eukaryotic nucleus typically utilize the h ... | 2009 | 19344747 |
| cross-presentation of viral and self antigens by skin-derived cd103+ dendritic cells. | skin-derived dendritic cells (dcs) include langerhans cells, classical dermal dcs and a langerin-positive cd103(+) dermal subset. we examined their involvement in the presentation of skin-associated viral and self antigens. only the cd103(+) subset efficiently presented antigens of herpes simplex virus type 1 to naive cd8(+) t cells, although all subsets presented these antigens to cd4(+) t cells. this showed that cd103(+) dcs were the migratory subset most efficient at processing viral antigens ... | 2009 | 19349986 |
| [effect of deletion mutant ips-1 on ifn-beta induction and its antiviral to hsv-1]. | the deletion mutant gene of ifn-beta promoter stimulator 1(deltaips-1) was constructed by depleting the amino acid region from 300 to 444 loci and the recombinant plasmid pef-bos-flag/deltaips-1 were constructed to study the effect of deletion mutant deltaips-1 to ifn-beta induction and its antiviral to hsv-1. | 2009 | 19351494 |
| stepwise evolution of the herpes simplex virus origin binding protein and origin of replication. | the herpes simplex virus replicon consists of cis-acting sequences, oris and oril, and the origin binding protein (obp) encoded by the ul9 gene. here we identify essential structural features in the initiator protein obp and the replicator sequence oris, and we relate the appearance of these motifs to the evolutionary history of the alphaherpesvirus replicon. our results reveal two conserved sequence elements in herpes simplex virus type 1, obp; the rvknl motif, common to and specific for all al ... | 2009 | 19351883 |
| physiological transgene regulation and functional complementation of a neurological disease gene deficiency in neurons. | the microtubule-associated protein tau (mapt) and alpha-synuclein (snca) genes play central roles in neurodegenerative disorders. mutations in each gene cause familial disease, whereas common genetic variation at both loci contributes to susceptibility to sporadic neurodegenerative disease. here, we demonstrate exquisite gene regulation of the human mapt and snca transgene loci and functional complementation in neuronal cell cultures and organotypic brain slices using the herpes simplex virus ty ... | 2009 | 19352323 |
| prevention of genital herpes simplex virus type 1 and 2 disease in mice immunized with a gd-expressing dominant-negative recombinant hsv-1. | cj9-gd is a novel herpes simplex virus (hsv) type 1 recombinant virus that is completely replication-defective, expresses high-levels of hsv-1 major antigen glycoprotein d (gd), and can function in trans to inhibit replication of wild-type hsv-1 and hsv-2 in co-infected cells. here, we show that immunization with cj9-gd elicits strong and long-lasting humoral and th1-like cellular immune responses against both hsv-1 and hsv-2. mice immunized with cj9-gd exhibited significant reductions in the ex ... | 2009 | 19357711 |
| immunomodulatory roles of topical dinitrochlorobenzene treatment in herpes simplex virus infection. | epicutaneous application of a contact sensitizer 2, 4-dinitro-1-chlorobenzene (dncb) is known to cause an inflammatory response and a hapten-specific t cell activation. we developed a highly stable gel formulation for dncb which is easy to apply to the skin. the present study focused on whether topical application of dncb gel produced immunomodulation with prophylactic effects against lethal herpes simplex virus infection (hsv) type 1. our results indicated that topical treatment with dncb gel i ... | 2009 | 19362169 |
| induction of sialyl-lex expression by herpes simplex virus type 1 is dependent on viral immediate early rna-activated transcription of host fucosyltransferase genes. | we have previously shown that varicella-zoster virus (vzv) and cytomegalovirus (cmv) infection of diploid human fibroblasts (hel) results in neo-expression of lewis antigens sialyl lewis x (sle(x)) and lewis y (le(y)), respectively, after transcriptional activation of different combinations of dormant human fucosyltransferase genes (fut1, fut3, fut5, and fut6), whose gene products are responsible for the synthesis of le antigens. here, we show that herpes simplex virus type 1 (hsv-1) also induce ... | 2009 | 19369700 |
| titration of variant hsv1-tk gene expression to determine the sensitivity of 18f-fhbg pet imaging in a prostate tumor. | because of its high selectivity and specificity for the imaging reporter probe 9-(4-(18)f-fluoro-3-[hydroxymethyl]butyl)guanine ((18)f-fhbg), the herpes simplex virus type 1 thymidine kinase (hsv1-tk) variant sr39tk is actively being studied as a pet reporter gene. we recently demonstrated the capability of using a prostate-specific transcriptional amplification pet reporter vector, adtsta-sr39tk, to target prostate cancer lymph node metastasis. however, one area that warrants further study is t ... | 2009 | 19372484 |
| [novel mechanism of herpes simplex virus type-i infection]. | 2009 | 19378786 | |
| pediatric herpes simplex virus keratitis. | to report the clinical characteristics and visual outcomes of pediatric herpes simplex virus (hsv) keratitis. | 2009 | 19387223 |
| hsv-1 encephalitis complicated by cerebral hemorrhage in an hiv-positive person. | although herpes simplex virus type 1 (hsv-1) is the most common cause of sporadic encephalitis in immunocompetent adults, it is an unusual cause of encephalitis in patients with hiv/aids. we report the case of a 56-year-old man with recently diagnosed hiv infection who presented with subacute mental status changes, fever, and temporal lobe abnormalities evident on brain imaging. results of a polymerase chain reaction assay of the cerebrospinal fluid were positive for hsv-1. his course was compli ... | 2009 | 19388182 |
| reactivated herpetic keratitis following laser in situ keratomileusis. | a 40-year-old woman presented with an unusual form of corneal herpetic reactivation 5 weeks after laser in situ keratomileusis. the cornea showed diffuse edema and a stromal ring infiltrate (disciform in appearance). oral antiviral and topical steroids in tapered dosages were administered. the infiltrate disappeared within 6 weeks. progressive improvement was also seen in all the parameters on pentacam scans, including the pachymetry, keratometry readings, and elevation on the anterior float. he ... | 2009 | 19393899 |
| synthesis and biological activity of amino acid esters of acyclovir. | 2009 | 19400144 | |
| induction of multiple pro-inflammatory cytokines by respiratory viruses and reversal by standardized echinacea, a potent antiviral herbal extract. | several viruses associated with upper respiratory diseases have been shown to stimulate the secretion of pro-inflammatory cytokines, including chemokines, sometimes in the absence of viral cytopathology. we evaluated the ability of a standardized preparation of the popular herbal medicine echinacea (echinaforce, an ethanol extract of herb and roots of e. purpurea, and containing known concentrations of marker compounds) to inhibit the viral induction of various cytokines in a line of human bronc ... | 2009 | 19409931 |
| reconstitution of uracil dna glycosylase-initiated base excision repair in herpes simplex virus-1. | herpes simplex virus-1 is a large double-stranded dna virus that is self-sufficient in a number of genome transactions. hence, the virus encodes its own dna replication apparatus and is capable of mediating recombination reactions. we recently reported that the catalytic subunit of the hsv-1 dna polymerase (ul30) exhibits apurinic/apyrimidinic and 5'-deoxyribose phosphate lyase activities that are integral to base excision repair. base excision repair is required to maintain genome stability as ... | 2009 | 19411250 |
| a case of herpetic epithelial keratitis after triamcinolone acetonide subtenon injection. | we report a case of herpes simplex virus (hsv) epithelial keratitis that developed after a subtenon triamcinolone acetonide (ta) injection. | 2009 | 19411969 |
| comparison of the pseudorabies virus us9 protein with homologs from other veterinary and human alphaherpesviruses. | pseudorabies virus (prv) us9 is a small, tail-anchored (ta) membrane protein that is essential for axonal sorting of viral structural proteins and is highly conserved among other members of the alphaherpesvirus subfamily. we cloned the us9 homologs from two human pathogens, varicella-zoster virus (vzv) and herpes simplex virus type 1 (hsv-1), as well as two veterinary pathogens, equine herpesvirus type 1 (ehv-1) and bovine herpesvirus type 1 (bhv-1), and fused them to enhanced green fluorescent ... | 2009 | 19420087 |
| 7-o-methylkaempferol and -quercetin glycosides from the whole plant of nervilia fordii. | five new 7-o-methylkaempferol and -quercetin glycosides, namely, nervilifordins a-e (1-5), were isolated from the whole plant of nervilia fordii, together with seven known flavonoids (6, 7, and 9-13) and one known coumarin (8). their structures were elucidated on the basis of extensive spectroscopic analyses, including hsqc, hmbc, roesy, and chemical methods. compounds 1-3 and 6-13 were evaluated for their anti-herpes simplex virus 1 (hsv-1) activity and cytotoxicity on african green monkey kidn ... | 2009 | 19422205 |
| the herpes simplex virus-induced demise of keratinocytes is associated with a dysregulated pattern of p63 expression. | p63 plays a pivotal role in the development and maintenance of stratified epithelial tissues. in an effort to gain insight into the pathogenic mechanisms of skin infections caused by hsv-1 and hsv-2, we determined the patterns of p63 expression in primary keratinocytes and in the hacat cell line. the levels of deltanp63alpha and a 50kda p73 isoform were decreased, bax-alpha remained unaffected, while the expressions of the bax-beta, tap63gamma and a 44.5kda p73 isoform were highly increased in b ... | 2009 | 19427396 |
| synthesis, antiviral activity, and stability of nucleoside analogs containing tricyclic bases. | a series of 3,9-dihydro-9-oxo-5h-imidazo[1,2-a]purine nucleosides (tricylic nucleosides) were synthesized from 9-[4-alpha-(hydroxymethyl)cyclopent-2-ene-1-alpha-yl]guanine (cbv) 5, (-)-beta-d-(2r,4r)-1,3-dioxolane-guanosine (dxg) 6, 3'-azido-3'-deoxy-guanosine (azg) 7, and 2'-c-methylguanosine 8. their in vitro activity against hiv and hcv was evaluated and correlated to their ability to degrade to their purine counterpart. | 2009 | 19433343 |
| what is your diagnosis? acute herpes simplex virus type 1 stomatitis. | 2009 | 19445307 | |
| sulfated k5 escherichia coli polysaccharide derivatives: a novel class of candidate antiviral microbicides. | antiviral microbicides, topical agents that prevent sexually transmitted infections, mainly work by blocking the interaction between viral proteins and cell surface components. in many instances, virus-cell interaction is mediated by cell surface heparan sulfate proteoglycans (hspgs). hspgs are exploited as attachment receptors by three sexually transmitted viruses: human immunodeficiency virus (hiv), herpes simplex virus (hsv) and human papilloma virus (hpv). since these viruses can either infe ... | 2009 | 19447134 |
| occurrence of hsv-1-induced pneumonitis in patients under standard immunosuppressive therapy for rheumatic, vasculitic, and connective tissue disease. | herpes simplex virus type-1 (hsv-1) has been described to cause respiratory tract infections in critically ill patients or in individuals that are immunocompromised. it is a continuing matter of debate under which circumstances hsv-1 is a relevant pathogen for pneumonitis. while its role during critical illness has been investigated by prospective interventional studies, comparatively little systematic data is available on the role of hsv-1 for pneumonitis in outpatients with autoimmune disease ... | 2009 | 19450259 |
| construction and cellular immune response induction of ha-based alphavirus replicon vaccines against human-avian influenza (h5n1). | several approaches are being taken worldwide to develop vaccines against h5n1 viruses; most of them, however, pose both practical and immunological challenges. one potential strategy for improving the immunogenicity of vaccines involves the use of alphavirus replicons and vp22, a herpes simplex type 1 (hsv-1) protein. in this study, we analysed the antigenic peptides and homogeneity of the ha sequences (human isolates of the h5n1 subtype, from 1997 to 2003) and explored a novel alphavirus replic ... | 2009 | 19450640 |
| isocoumarin glucosides from the scale insect fungus torrubiella tenuis bcc 12732. | three new isocoumarin glucosides (1, 3, and 4), 6,8-dihydroxy-3-methylisocoumarin (2), and 6,8-dihydroxy-3-hydroxymethylisocoumarin (5) were isolated from the scale insect pathogenic fungus torrubiella tenuis bcc 12732. structures of these compounds were elucidated using nmr spectroscopic and ms spectrometric analyses. compound 5 exhibited moderate anti-hsv-1 and antimycobacterial activities with ic(50) and mic values of 50 and 25 microg/ml, respectively. | 2009 | 19456117 |
| [diagnostics of genital herpesvirus infection in women with tubal-peritoneal infertility]. | optimization of diagnostics of genital herpesvirus infection (herpes simplex virus types 1 and 2, cytomegalovirus [cmv]) in women with tubal-peritoneal infertility on the basis of detection of viral material in various parts of genital system using polymerase chain reaction (pcr) and revealing pathogenetically significant systemic abnormalitites of cytokine- and nitroxidergic regulation. | 2009 | 19459485 |
| prevalence of human herpes virus types 1-7 in the semen of men attending an infertility clinic and correlation with semen parameters. | to determine the prevalence of herpes viruses in the semen of an asymptomatic male cohort with and without infertility problems and its association with altered semen parameters. | 2009 | 18565516 |
| pet imaging of heat-inducible suicide gene expression in mice bearing head and neck squamous cell carcinoma xenografts. | the ability to achieve tumor selective expression of therapeutic genes is an area that needs improvement for cancer gene therapy to be successful. one approach to address this is through the use of promoters that can be controlled by external means, such as hyperthermia. in this regard, we constructed a replication-deficient adenovirus that consists of a mutated herpes simplex virus 1 thymidine kinase (mtk) fused to enhanced green fluorescent protein (egfp) under the control of the full-length h ... | 2009 | 18758434 |
| [autoradiography reporter gene (ad5-tk carrying hsv1-tk gene) expression imaging in rat myocardium]. | radionuclide imaging of reporter gene expression holds promise for noninvasive monitoring of gene therapy. herpes simplex virus 1-thymidine kinase (hsv1-tk) has been successfully applied to the tumor tissue.we explored the feasibility of the expression imaging of hsv1-tk reporter gene in rat myocardium by using spect reporter probe (131)i-2'-fluoro-2'-deoxy-1-beta-d-arabinofuranosyl-5-iodouracil ((131)i-fiau) and autoradiography (arg). | 2009 | 20137546 |
| verbascoside isolated from lepechinia speciosa has inhibitory activity against hsv-1 and hsv-2 in vitro. | verbascoside has been isolated form l. speciosa after several different chromatographic methods. after its purification, the structure has been unequivocally established using modern spectroscopic techniques. as for the antiviral activity, the maximum non toxic concentration has been established and this concentration has been used in the anti herpes assay, in vitro. mechanism of action for this molecule regarding the anti-herpes activity has been studied encompassing the following assays: viruc ... | 2009 | 20120109 |
| a limited innate immune response is induced by a replication-defective herpes simplex virus vector following delivery to the murine central nervous system. | herpes simplex virus type 1 (hsv-1)-based vectors readily transduce neurons and have a large payload capacity, making them particularly amenable to gene therapy applications within the central nervous system (cns). because aspects of the host responses to hsv-1 vectors in the cns are largely unknown, we compared the host response of a nonreplicating hsv-1 vector to that of a replication-competent hsv-1 virus using microarray analysis. in parallel, hsv-1 gene expression was tracked using hsv-spec ... | 2009 | 20095947 |
| antiviral properties of antineoplastic drugs. from herpes simplex-1 disappearance to a wide antiviral action: a serendipity case report. | herein we report the clinical case of a 46 year old female affected by lung cancer and cerebral metastases who showed, one month after the beginning of an oncologic therapy with vinorelbine and cisplatin, the complete remission of periodic (every 15 days) recurrences of herpetic mouth lesions. more than one year after the disappearance of the herpetic lesions, no further evidence of them were found. subsequently, specific laboratory investigations, together with another case report that is prese ... | 2009 | 20578421 |
| sexually transmitted diseases and anorectum. | sexually transmitted diseases (std) are a major public health problem because their incidence is increasing worldwide despite prevention campaigns and because they raise the risk of hiv infection. anorectal localisations of std are common among men who have sex with men (msm) but can also be seen among heterosexuals (men or women). transmission of such infections is due to anal sex or to other sexual behaviours like "fisting". although some pathogens (like human papillomavirus-hpv) are common in ... | 2009 | 20163035 |
| a delicate balance: risk factors for acquisition of bacterial vaginosis include sexual activity, absence of hydrogen peroxide-producing lactobacilli, black race, and positive herpes simplex virus type 2 serology. | the etiology of bacterial vaginosis (bv) is poorly understood, but better definition of the risk factors associated with its acquisition should improve our understanding of this complex disease entity. | 2008 | 17989585 |
| antiviral activity and mode of action of a peptide isolated from sorghum bicolor. | in this paper, we describe the purification of an antiviral peptide from seeds of sorghum bicolor l. by a procedure that included gel filtration, ion exchange, and high-performance liquid chromatography (hplc) in a reversed-phase column. its molecular weight, determined by chromatographic mobility on the shim-pack diol-150 gel permeation column in hplc, was found to be 2000da. the peptide designated 2kd peptide strongly inhibited the replication of herpes simplex virus type 1 (hsv-1), dose-depen ... | 2008 | 17890069 |
| herpes simplex virus type 1 preferentially targets human colon carcinoma: role of extracellular matrix. | viral therapy of cancer (viral oncolysis) is dependent on selective destruction of the tumor tissue compared with healthy tissues. several factors, including receptor expression, extracellular components, and intracellular mechanisms, may influence viral oncolysis. in the present work, we studied the potential oncolytic activity of herpes simplex virus type 1 (hsv-1), using an organ culture system derived from colon carcinoma and healthy colon tissues of mouse and human origin. hsv-1 infected no ... | 2008 | 17977977 |
| translocation and colocalization of icp4 and icp0 in cells infected with herpes simplex virus 1 mutants lacking glycoprotein e, glycoprotein i, or the virion host shutoff product of the ul41 gene. | in wild-type herpes simplex virus 1-infected cells, the major regulatory protein icp4 resides in the nucleus whereas icp0 becomes dynamically associated with proteasomes and late in infection is translocated and dispersed in the cytoplasm. inhibition of proteasomal function results in retention or transport of icp0 to the nucleus. we report that in cells infected with mutants lacking glycoprotein e (ge), glycoprotein i (gi), or the product of the u(l)41 gene, both icp4 and icp0 are translocated ... | 2008 | 18057247 |
| antiviral and immunomodulatory effect of a lyophilized extract of capparis spinosa l. buds. | herpes simplex virus type 1 (hsv-1) and herpes simplex virus type 2 (hsv-2) are common human pathogens that in particular cases can also cause severe problems especially in immunodeficient patients. the present paper reports the antiviral and immunomodulatory properties of a methanolic extract of c. spinosa buds (cap), rich in flavonoids, including several quercetin and kaempferol glycosides. in particular we have investigated whether the in vitro exposure of human peripheral blood mononuclear c ... | 2008 | 18058988 |
| a conserved domain of herpes simplex virus icp34.5 regulates protein phosphatase complex in mammalian cells. | icp34.5, encoded by herpes simplex virus 1, is a protein phosphatase 1 (pp1) regulatory subunit that mediates dephosphorylation of the alpha subunit of translation initiation factor 2 (eif2alpha). however, the mechanism of its action remains poorly understood. here, we show that amino acid substitutions in the arginine-rich motif have differential effects on icp34.5 activity. the phenotypes parallel with viral protein synthesis and cytopathic effects in virus infected cells. besides the consensu ... | 2008 | 18068675 |
| terpenoids and phenolics from plectranthus strigosus, bioactivity screening. | further studies on plectranthus sp. (lamiaceae) led to the isolation of terpenes and phenol esters from plectranthus strigosus benth. ent-16-kauren-19-ol (1), ent-16-kauren-19-oic acid (2), xylopic acid (3), xylopinic acid (4), hinokiol (5), 4 beta,6 beta-dihydroxy-1 alpha,5 beta(h)-guai-9-ene (6) 4 beta,6 beta-dihydroxy-1 alpha,5 beta(h)-guai-10(14)-ene (7), mixtures of hexacosan-1,26-diol and octacosan-1,28-diol ferulate diesters (8), of esters from ferulic acid and fatty alcohols (9) and of e ... | 2008 | 18075900 |
| the essential human cytomegalovirus gene ul52 is required for cleavage-packaging of the viral genome. | replication of human cytomegalovirus (hcmv) produces large dna concatemers of head-to-tail-linked viral genomes that upon packaging into capsids are cut into unit-length genomes. the mechanisms underlying cleavage-packaging and the subsequent steps prior to nuclear egress of dna-filled capsids are incompletely understood. the hitherto uncharacterized product of the essential hcmv ul52 gene was proposed to participate in these processes. to investigate the function of pul52, we constructed a delt ... | 2008 | 18077717 |
| binding of icp4, tata-binding protein, and rna polymerase ii to herpes simplex virus type 1 immediate-early, early, and late promoters in virus-infected cells. | the binding of herpes simplex virus type 1 icp4, tata-binding protein (tbp), and rna polymerase ii (polii) to the promoter regions of representative immediate-early (ie) (icp0), early (e) (thymidine kinase [tk]), and late (l) (glycoprotein c [gc]) genes on the viral genome was examined as a function of time postinfection, viral dna replication, cis-acting sites for tfiid in the tk and gc promoters, and genetic background of icp4. the binding of tbp and polii to the ie icp0 promoter was independe ... | 2008 | 18094162 |
| nuclear egress and envelopment of herpes simplex virus capsids analyzed with dual-color fluorescence hsv1(17+). | to analyze the assembly of herpes simplex virus type 1 (hsv1) by triple-label fluorescence microscopy, we generated a bacterial artificial chromosome (bac) and inserted eukaryotic cre recombinase, as well as beta-galactosidase expression cassettes. when the bac phsv1(17(+))bluelox was transfected back into eukaryotic cells, the cre recombinase excised the bac sequences, which had been flanked with loxp sites, from the viral genome, leading to hsv1(17(+))bluelox. we then tagged the capsid protein ... | 2008 | 18160444 |
| c-reactive protein mediates the effect of apolipoprotein e on cytomegalovirus infection. | although the apolipoprotein (apoe)-epsilon4 allele has been shown to determine the outcome of several infections, its relationship with cytomegalovirus (cmv) has not been explored. we examine whether apoe determines cmv and herpes simplex virus type 1 (hsv-1) antibody levels and assess whether c-reactive protein (crp) mediates any observed relationships. | 2008 | 18171282 |
| characterization of mre11 loss following hsv-1 infection. | herpes simplex virus induces the activation of the cellular dna double strand break response pathway dependent upon initiation of viral dna replication. the mrn complex, consisting of mre11, rad50 and nbs1, is an essential component of the dna double strand break response and other reports have documented its presence at sites of viral dna replication, interaction with icp8 and its contribution to efficient viral dna replication. during our characterization of the dsb response following infectio ... | 2008 | 18177684 |
| dissecting the structural determinants of the interaction between the human cytomegalovirus ul18 protein and the cd85j immune receptor. | ul18 is a glycoprotein encoded by the human cytomegalovirus genome and is thought to play a pivotal role during human cytomegalovirus infection, although its exact function is still a matter of debate. ul18 shares structural similarity with mhc class i and binds the receptor cd85j on immune cells. besides ul18, cd85j binds mhc class i molecules. the binding properties of cd85j to mhc class i molecules have been thoroughly studied. conversely, very little information is available on the cd85j/ul1 ... | 2008 | 18178836 |
| in vivo evaluation of the uptake of [(123)i]fiau, [(123)i]ivfru and [(123)i]ivfau by normal mouse brain: potential for noninvasive assessment of hsv-1 thymidine kinase gene expression in gliomas. | radioiodinated 5-iodo-1-(2-fluoro-2-deoxy-beta-d-arabinofuranosyl)uracil (f *iau) is most commonly used for noninvasive assessment of herpes simplex virus type 1 thymidine kinase (hsv-1-tk) gene expression. however, it does not permeate the intact blood-brain barrier (bbb) because of its moderate lipophilicity. in this work, three iodo-nucleosides, fiau, ivfru, and ivfau, were radiolabeled with iodine-123 and tested for permeation of the bbb in mice and for potential measurement of hsv-1-tk gene ... | 2008 | 18188770 |
| evaluation of the t-rex transcription switch for conditional expression and regulation of hsv-1 vectors. | herpes simplex virus 1 (hsv-1) strain ang and angpath were cloned as bacterial artificial chromosome (bac). two different types of bac genomes were obtained. bac genomes of type i contained the bac replicon at the intended target region between the genes of ul48 and ul49. in bac genomes of type ii, the bac sequences were found to be aberrantly fused between the termini of the hsv-1 genome. both the bac types were used to establish a conditional gene expression system for hsv-1 by flp recombinase ... | 2008 | 18193347 |
| influenza virus infection and risk of acute myocardial infarction. | increasing evidences have shown that pathogens might promote atherosclerosis and trigger acute myocardial infarction (ami). but the conclusions from various studies on the correlation between previous influenza virus (iv) infection and ami were inconsistent. we conducted a case-control study to assess the association of previous iv infection and ami. questionnaire survey was conducted to collect information about demographic characteristics and heart disease risk factors. fasting blood sample wa ... | 2008 | 18568394 |
| inhibition of herpes simplex virus types 1 and 2 in vitro infection by sulfated derivatives of escherichia coli k5 polysaccharide. | herpes simplex virus type 1 (hsv-1) and hsv-2 are neurotropic viruses and common human pathogens causing major public health problems such as genital herpes, a sexually transmitted disease also correlated with increased transmission and replication of human immunodeficiency virus type 1 (hiv-1). therefore, compounds capable of blocking hiv-1, hsv-1, and hsv-2 transmission represent candidate microbicides with a potential added value over that of molecules acting selectively against either infect ... | 2008 | 18573926 |
| comparison of nested-polymerase chain reaction and virus culture for the diagnosis of genital herpes simplex virus infection. | this study was performed to compare nested-polymerase chain reaction (pcr) with viral culture as a diagnostic tool for genital herpes simplex virus (hsv) infection in a sexually transmitted infection (sti) clinic in singapore. | 2008 | 18581019 |
| ocular hsv-1 latency, reactivation and recurrent disease. | ocular infection with hsv-1 continues to be a serious clinical problem despite the availability of effective antivirals. primary infection with hsv-1 can involve ocular and adenaxial sites and can manifest as blepharitis, conjunctivitis, or corneal epithelial keratitis. after initial ocular infection, hsv-1 can establish latent infection in the trigeminal ganglia for the lifetime of the host. during latency, the viral genome is retained in the neuron without producing viral proteins. however, ab ... | 2008 | 18584563 |
| identification of a novel higher molecular weight isoform of usp7/hausp that interacts with the herpes simplex virus type-1 immediate early protein icp0. | the herpes simplex virus type-1 (hsv-1) regulatory protein icp0, a ring-finger e3 ubiquitin ligase, stimulates the onset of viral lytic replication and the reactivation of quiescent viral genomes from latency. like many ubiquitin ligases icp0 induces its own ubiquitination, a process that can lead to its proteasome-dependent degradation. icp0 counteracts this activity by recruiting the cellular ubiquitin-specific protease usp7/hausp. here we show that icp0 can also interact with a previously uni ... | 2008 | 18590780 |
| the enhanced dna replication fidelity of a mutant herpes simplex virus type 1 dna polymerase is mediated by an improved nucleotide selectivity and reduced mismatch extension ability. | we previously demonstrated that a recombinant herpes simplex virus containing a mutation within the finger domain of dna polymerase replicated dna with increased fidelity. in this study, we demonstrate that, compared with wild-type polymerase, the mutant enzyme exhibited improved nucleotide selectivity and a reduced ability to extend from mismatched primer termini, which would contribute to the increased dna replication fidelity. | 2008 | 18596099 |
| comprehensive characterization of extracellular herpes simplex virus type 1 virions. | the herpes simplex virus type 1 (hsv-1) genome is contained in a capsid wrapped by a complex tegument layer and an external envelope. the poorly defined tegument plays a critical role throughout the viral life cycle, including delivery of capsids to the nucleus, viral gene expression, capsid egress, and acquisition of the viral envelope. current data suggest tegumentation is a dynamic and sequential process that starts in the nucleus and continues in the cytoplasm. over two dozen proteins are as ... | 2008 | 18596102 |
| exercise stress increases susceptibility to influenza infection. | exercise stress is associated with an increased risk for upper respiratory tract infection (urti) while moderate exercise has been associated with a decreased risk. we have shown that exercise stress can increase susceptibility (morbidity, symptom severity and mortality) to hsv-1 respiratory infection, but there is little evidence on the effects of stressful exercise on susceptibility to the principal etiological agents of human respiratory infections, including influenza viruses. this study exa ... | 2008 | 18616997 |
| thymidine kinase sequence analysis of herpes simplex virus type 1 strains present in different compartments in an atypical impetiginous rash on the lesional skin of a burn patient. | we report the case of a 23-year-old male burn patient with an unusual herpes simplex virus (hsv) skin manifestation. the clinical symptoms and results of hsv type 1 (hsv-1) ul23 polymorphism analysis from saliva and lesional skin underscores the need for performing molecular analysis of hsv-1 infections in burned patients presenting unusual skin lesions. | 2008 | 18632917 |
| lymphoid-related cd11c+ cd8alpha+ dendritic cells are involved in enhancing herpes simplex virus type 1 latency. | the mechanism(s) by which herpes simplex virus type 1 (hsv-1) latency is established in neurons is not known. in this study, we examined the effect of dendritic cells (dcs) on the level of hsv-1 latency in trigeminal ganglia (tgs) of ocularly infected balb/c and c57bl/6 mice. we found that immunization of wild-type mice with fms-like tyrosine kinase 3 ligand (flt3l) dna, which increases the number of dcs, increased the amount of latency in infected mice. conversely, depletion of dcs was associat ... | 2008 | 18667491 |
| gender-dependent hla-dr-restricted epitopes identified from herpes simplex virus type 1 glycoprotein d. | in recent clinical trials, a herpes simplex virus (hsv) recombinant glycoprotein d (gd) vaccine was more efficacious in woman than in men. here we report six hla-dr-restricted t-cell gd epitope peptides that bind to multiple hla-dr (dr1, dr4, dr7, dr13, dr15, and drb5) molecules that represent a large proportion of the human population. four of these peptides recalled naturally primed cd4(+) t cells in up to 45% of the 46 hsv-seropositive, asymptomatic individuals studied. for the gd(49-82), gd( ... | 2008 | 18667634 |
| ly49h is necessary for genetic resistance to murine cytomegalovirus. | natural killer (nk) cells play critical roles in antiviral immunity. while the importance of effector mechanisms such as interferons has been demonstrated through knockout mice, specific mechanisms of how viruses are recognized and controlled by nk cells are less well defined. previous genetic studies have mapped the resistance genes for murine cytomegalovirus (mcmv), herpes simplex virus-1 (hsv-1), and ectromelia virus to the nk gene complex on murine chromosome 6, a region containing the polym ... | 2008 | 18668236 |
| involvement of hvem receptor in activation of nuclear factor kappab by herpes simplex virus 1 glycoprotein d. | the uv-inactivated herpes simplex virus 1 (hsv-1) and glycoprotein d (gd) of hsv-1 have been shown to activate nuclear factor kappab (nf-kappab) in u937 cells, but mechanisms involved in this activation have not been elucidated. here we report that: (i) uv-inactivated hsv-1 induced an increased nf-kappab activation in cells expressing human hvem (for herpesvirus entry mediator) at surface level, naturally or following stable transfection, but not in cells in which this receptor was not detected ... | 2008 | 18671825 |
| construction and production of recombinant herpes simplex virus vectors. | virus vectors have been employed as gene transfer vehicles for various pre-clinical and clinical gene therapy applications. replication-competent herpes simplex virus (hsv) vectors that replicate specifically in actively dividing glial tumor cells have been used in phase i-ii human trials in patients with glioblastoma multiforme (gbm), a fatal form of brain cancer. research during the last decade on the development of hsv vectors has resulted in the engineering of recombinant vectors that are to ... | 2008 | 18679619 |
| herpes simplex virus load in bronchoalveolar lavage fluid is related to poor outcome in critically ill patients. | to evaluate the relationship between the hsv-1 and -2 loads in bal fluid (balf) and clinical outcome. | 2008 | 18679655 |
| [the genes associated with geldanamycin inhibiting replication of herpes simplex virus type 1]. | geldanamycin (ga), an ansamycin antibiotic specifically binding heat shock protein 90 (hsp90), exhibits a broad-spectrum antiviral effect. herpes simplex virus type 1(hsv-1) replication in vitro was significantly inhibited by ga treatment. to explore the antiviral mechanism of ga against hsv-1, the 7267-spot human long oligonucleotide microarrays were applied to investigate the genes which might involved in the antiviral activity of ga in hela cells infected by hsv-1. meanwhile, the reverse regu ... | 2008 | 18683558 |
| icp34.5-dependent and -independent activities of salubrinal in herpes simplex virus-1 infected cells. | the small molecule salubrinal has antiviral activity against herpes simplex virus-1 (hsv-1) and inhibits dephosphorylation of eif2 alpha mediated by the hsv-1 protein icp34.5. we investigated whether salubrinal's activities in infected cells depend on icp34.5. an icp34.5 deletion mutant was as sensitive as wild type hsv-1 to salubrinal inhibition of plaque formation in vero cells. however, salubrinal induced formation of syncytia in infected vero cells, which was enhanced by icp34.5 mutations. e ... | 2008 | 18684481 |
| human cytomegalovirus ul18 utilizes us6 for evading the nk and t-cell responses. | human cytomegalovirus (hcmv) us6 glycoprotein inhibits tap function, resulting in down-regulation of mhc class i molecules at the cell surface. cells lacking mhc class i molecules are susceptible to nk cell lysis. hcmv expresses ul18, a mhc class i homolog that functions as a surrogate to prevent host cell lysis. despite a high level of sequence and structural homology between ul18 and mhc class i molecules, surface expression of mhc class i, but not ul18, is down regulated by us6. here, we desc ... | 2008 | 18688275 |