Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| espfu, a type iii-translocated effector of actin assembly, fosters epithelial association and late-stage intestinal colonization by e. coli o157:h7. | enterohaemorrhagic escherichia coli (ehec) o157:h7 induces filamentous actin-rich 'pedestals' on intestinal epithelial cells. pedestal formation in vitro requires translocation of bacterial effectors into the host cell, including tir, an ehec receptor, and espf(u), which increases the efficiency of actin assembly initiated by tir. while inactivation of espf(u) does not alter colonization in two reservoir hosts, we utilized two disease models to explore the significance of espf(u)-promoted actin ... | 2008 | 18067584 |
| expression of the capsid protein of porcine circovirus type 2 in lactococcus lactis for oral vaccination. | diseases associated with porcine circovirus type 2 (pcv2) infections are becoming a major problem for the swine industry worldwide. the capsid protein (cap) of pcv2 is an antigen important for both early diagnosis and development of vaccines. in this study, lactococcus lactis was used as vehicle to deliver the pcv2 antigen in an attempt to develop oral vaccine. a cap gene with a deleted nuclear localization signal sequence (dcap) was cloned into an escherichia coli/l. lactis shuttle vector psec: ... | 2008 | 18406475 |
| renal cytokine profile in an endotoxemic porcine model. | in animals exposed to acute endotoxemia with lipopolysaccharide (lps), high levels of cytokines are found in the kidney. the objective of this study is to determine whether the high renal content of tnf-alpha, il-1beta, il-10 and il-1 receptor antagonist (il-1ra) is due to glomerular filtration and reabsorption, or whether the cytokines are produced locally in the kidney. | 2008 | 18419714 |
| transcytosis of f4 fimbriae by villous and dome epithelia in f4-receptor positive pigs supports importance of receptor-dependent endocytosis in oral immunization strategies. | very few antigens have been described that induce an intestinal immunity when given orally. our laboratory demonstrated that oral administration of isolated f4 (k88) fimbriae of escherichia coli to f4-receptor positive (f4r(+)) pigs induces protective mucosal immunity against challenge infection. however, presence of f4-receptors (f4r) on villous enterocytes is a prerequisite for inducing the immune response, as no f4-specific antibody-secreting cells (asc) can be induced in f4r(-) pigs. in this ... | 2008 | 18423889 |
| lysozyme transgenic goats' milk influences gastrointestinal morphology in young pigs. | transgenesis provides a method of expressing novel proteins in milk to increase the functional benefits of milk consumption. transgenic goats expressing human lysozyme (hlz) at 67% of the concentration in human breast milk were produced, thereby enhancing the antimicrobial properties of goats' milk. the objective of this study was to investigate the impact of pasteurized milk containing hlz on growth, the intestinal epithelium, and an enteropathogenic escherichia coli (epec) infection in young w ... | 2008 | 18424602 |
| [characteristics and immunogenicity of the n-terminal and c-terminal recombinants of pasteurella multocida toxin]. | in this study, five fragments of recombinant subunit pasteurella multocida toxin (pmt) were constructed. only pet28a-n1518 and pet28b-c2115 could be expressed efficiently in escherichia coli. the molecular weight of the fusion proteins was 57 kda and 78 kda .western blot confirmed that the two proteins could specifically react with antiserum against pasteurella multocida toxin. no mice died after the intraperitoneal administration of these two proteins with the dose of 200 microg per mouse, but ... | 2008 | 18438004 |
| [baculovirus expression and establishment of the indirect elisa for the ha gene of swine influenza virus h1 subtype]. | the hemagglutinin (ha) gene fragment of swine influenza virus a/swine/guangdong/lm/05(h1n1) was amplified with ha gene specific primers and cloned into baculovirus transfer plasmid pfastbacgp67b. the recombinant plasmid pfastbacgp67b-h1 was identified by restriction enzyme digestion and gene sequencing. following the transformation of dh10bac escherichia coli component cells by pfastbacgp67b-h1, recombinant bacmids rbacmid-h1 were identified by blue/white selection and pcr analysis. then recombi ... | 2008 | 18438005 |
| loss of virulence genes in escherichia coli populations during manure storage on a commercial swine farm. | confined livestock production farms typically store their wastes prior to land application. here, we employed three complementary approaches to evaluate changes in the population structure and stability of virulence genes in escherichia coli during manure storage on a commercial farm that housed healthy swine. isolates were genotyped by repetitive extragenic palindromic pcr using the boxa1r primer and evaluated for the presence of selected virulence genes by pcr. isolates obtained from the manur ... | 2008 | 18441108 |
| new adhesin of enteroaggregative escherichia coli related to the afa/dr/aaf family. | enteroaggregative escherichia coli (eaec) is an important cause of diarrhea worldwide. we analyzed 17 danish eaec strains, isolated in the course of a case control study, for phenotypic and genotypic properties. the strains belonged to at least 14 different serotypes. using pcr to investigate the prevalence of various putative virulence genes, we found that all but two strains were typical eaec, as they harbored all or part of the previously described aggr regulon. the majority of the strains ha ... | 2008 | 18443096 |
| investigation of the porcine muc13 gene: isolation, expression, polymorphisms and strong association with susceptibility to enterotoxigenic escherichia coli f4ab/ac. | enterotoxigenic escherichia coli (etec) f4ab and f4ac are major determinants of piglet diarrhoea. the locus for the etec f4ab/ac receptor has been mapped to ssc13q41. muc13 is a transmembrane mucin expressed predominantly in the epithelial surface of the gastrointestinal tract and the muc13 gene was assigned to ssc13q41, supporting it as a positional candidate gene for the etec f4ab/ac receptor. we herein determined the complete 2679-bp cdna of pig muc13, and proved that it was most highly expre ... | 2008 | 18454803 |
| antibiotic resistance integrons and extended-spectrum {beta}-lactamases among enterobacteriaceae isolates recovered from chickens and swine in portugal. | to investigate the diversity of integrons and extended-spectrum beta-lactamases (esbls) among enterobacteriaceae from chickens and swine in portugal and analyse the clonal relationships between portuguese esbl-producing isolates of animal and human origin. | 2008 | 18456652 |
| [gene cloning, expression and activity detection of porcine interleukin-18 mature protein in escherichia coli]. | porcine interleukin-18 mature protein gene was amplified from porcine spleen cells by rt-pcr. pcr product was cloned into the t vector pgem-t for sequencing. the nucleotide sequence of this gene was 474 bp. then, it was subcloned into the prokaryotic expressing plasmid vector pgex6p-1 and transformed into host e. coli strain bl21 for expression. the expression of pil-18 mature protein gene was identified by sds-page .the expression product was fusion protein with molecular weight of 45 kd and th ... | 2008 | 18464603 |
| using data on resistance prevalence per sample in the surveillance of antimicrobial resistance. | in most existing antimicrobial resistance monitoring programmes, one single bacterial colony from each collected sample is susceptibility tested against a panel of antimicrobials. detecting the proportion of colonies resistant to different antimicrobials in each sample can provide quantitative data on antimicrobial resistance (resistance prevalence per sample). | 2008 | 18490375 |
| prevalence of quinolone resistance mechanisms and associations to minimum inhibitory concentrations in quinolone-resistant escherichia coli isolated from humans and swine in denmark. | prevalence of quinolone resistance mechanisms and associations to minimum inhibitory concentrations (mics) of nalidixic acid (nal) and ciprofloxacin (cip) were investigated in 124 escherichia coli isolated from humans (n=85) and swine (n=39) in denmark. the collection included 59 high-level cip-resistant isolates (mic >or= 4) from human (n=51) and pig origin (n=8) and 65 low-level cip-resistant isolates (mic >or= 0.125) from human (n=34) and pig origin (n=31). resistance by target modification w ... | 2008 | 18500921 |
| antimicrobial resistance in generic escherichia coli isolated from swine fecal samples in 90 alberta finishing farms. | the objective of this study was to determine the prevalence of antimicrobial resistance in generic escherichia coli isolates obtained from 90 alberta finisher swine farms. up to 5 isolates were obtained from each of 269 pooled fecal samples and were classified as susceptible or resistant according to clinical and laboratory standards institute guidelines. of the 1322 isolates, 166 (12.6%) were susceptible to all 15 antimicrobials. no resistance to amikacin, ceftiofur, ceftriaxone, or ciprofloxac ... | 2008 | 18505207 |
| prevalence and patterns of antimicrobial resistance of fecal escherichia coil among pigs on 47 farrow-to-finish farms with different in-feed medication policies in ontario and british columbia. | the main objectives of this study were to determine the prevalence and patterns of antimicrobial resistance in pigs on farms that medicated swine ration and those that did not. a total of 940 isolates of escherichia coli from 188 pooled fecal samples obtained from weaner and finisher pigs on 47 farrow-to-finish swine farms (34 farms used in-feed medication and 13 did not) were tested for susceptibility to 21 antimicrobials using a breakpoint concentration method. the prevalence of resistance var ... | 2008 | 18505210 |
| the effect of indomethacin on systemic and renal hemodynamics in neonatal piglets during experimental endotoxemia. | systemic and renal hemodynamics are affected by prostaglandin production during endotoxemia. to study indomethacin effects on endotoxinemia in a neonatal piglet model, sixteen 7-10 day old piglets were anesthetized, ventilated, and catheterized. mean arterial pressure (map), heart rate (hr), and urine output were continuously monitored. endotoxin (0.06 mcg/kg) was injected after baseline measurements. we studied two groups with either endotoxinemia alone (n = 7) or an additional indomethacin inf ... | 2008 | 18509659 |
| phylogenetic background of attaching and effacing escherichia coli isolates from animals. | detection and distribution of eae gene in forty-four attaching and effacing escherichia coli (aeec) strains of animal origin were investigated. association of distinct intimin alleles with phylogenetic background were assessed among strains in comparison with different serogroups. phylogenetic analysis showed that 31 ehec/eae+ stec strains belong to groups a, b1 and e, 13 epec strains segregated in b1 and b2. moreover, group a possessed the eae gamma2/theta type, group b1 the eae beta1, eae kapp ... | 2008 | 18509740 |
| a laboratory study of survival of selected microorganisms after heat treatment of biowaste used in biogas plants. | the aim of the study was to assess the effect of pasteurisation, as set by the european regulation ec 1774/2002, on selected pathogens and indicator organisms. unpasteurised substrate (biowaste), including animal by-products from a full-scale biogas plant was heat treated under laboratory conditions at 70 degrees c and 55 degrees c for 30 min and 60 min. heat treatment at 55 degrees c for 60 min was not sufficient to achieve a hygienically acceptable product. heat treatment at 70 degrees c for 3 ... | 2008 | 18513960 |
| comparison of swabbing and destructive methods for microbiological pig carcass sampling. | to compare the belgian swabbing sampling method for pig carcasses with the reference destructive method with regard to escherichia coli and aerobic plate counts, salmonella and campylobacter prevalence and their relationship. | 2008 | 19241527 |
| investigation of bioaerosols released from swine farms using conventional and alternative waste treatment and management technologies. | microbial air pollution from concentrated animal feeding operations (cafos) has raised concerns about potential public health and environmental impacts. we investigated the levels of bioaerosols released from two swine farms using conventional lagoon-sprayfield technology and ten farms using alternative waste treatment and management technologies in the united states. in total, 424 microbial air samples taken at the 12 cafos were analyzed for several indicator and pathogenic microorganisms, incl ... | 2008 | 19192808 |
| genetic variation at the alpha-1-fucosyltransferase (fut1) gene in asian wild boar and chinese and western commercial pig breeds. | escherichia coli f18 bacteria producing enterotoxins and/or shigatoxin (etec/stec) are main pathogens that cause oedema disease and postweaning diarrhoea in piglets, and alpha-1-fucosyltransferase (fut1) gene has been identified as a candidate gene for controlling the expression of etec f18 receptor. the genetic variations at nucleotide position 307 in open reading frame of fut1 gene in one wild boar breed and 20 western commercial and chinese native pig breeds were investigated by polymerase ch ... | 2008 | 19134079 |
| [construction and immunogenicity of a genetic engineered strain expressing nontoxic st1-lt(b)-alpha-beta fusion protein against diarrhea of piglet]. | we constructed a recombinant strain bl21 (de3) (petst3ltbalphabeta) including st1-lt(b)-alpha-beta fusion gene via molecular technology. the sds-page and western blotting indicated that the st1-lt(b)-alpha-beta fusion protein was highly expressed in escherichia coli and the molecular weight of the fusion protein was about 110 kd. the recombinant strain was induced in different concentrations of lactose and different aeration rate. the optimal culture conditions in 20 l fermentor were 1% inoculat ... | 2008 | 18998533 |
| conserved stx2 phages from escherichia coli o103:h25 isolated from patients suffering from hemolytic uremic syndrome. | one of the main virulence factors produced by shiga toxin-producing escherichia coli is the shiga toxin (stx), which is encoded on lambdoid phages (stx phage). in norway, an outbreak of hemorrhagic colitis and hemolytic uremic syndrome (hus) caused by e. coli o103:h25 was reported during the winter of 2006, but stx(2)-positive isolates were only retrieved from two human samples. | 2008 | 19014273 |
| development and validation of a recombinant capsid protein-based elisa for detection of antibody to porcine circovirus type 2. | porcine circovirus type 2 (pcv2) has been recently associated with a number of disease syndromes, especially postweaning multisystemic wasting disease (pmws). herein, an alternative indirect enzyme-linked immunosorbent assay (elisa) for detection of pcv2 antibody was developed using nuclear localization signal-truncated capsid protein of pcv2 produced in escherichia coli (cap elisa). this assay was validated by comparison with an indirect immunofluorescence assay (iif) and a pcv2-based elisa. th ... | 2008 | 17467754 |
| the consequences of non-starch polysaccharide solubility and inclusion level on the health and performance of weaned pigs challenged with enterotoxigenic escherichia coli. | the inclusion of insoluble nsp (insp) in weaner pig diets has been reported to decrease post-weaning colibacillosis (pwc). conversely, soluble nsp (snsp) have been shown to exacerbate pwc. the present study investigated the effect of nsp solubility and inclusion level on the health and performance of newly weaned pigs challenged with enterotoxigenic escherichia coli (etec), using nsp sources known not to affect digesta viscosity, in a 2 x 2 x 2 factorial combination of nsp solubility (insp v. sn ... | 2008 | 17761008 |
| molecular cloning and functional characterization of a histidine decarboxylase from staphylococcus capitis. | histamine intoxication is probably the best known toxicological problem of food-borne disease. a histamine-producing staphylococcus capitis strain has been isolated from a cured meat product. the aim of this study was to gain deeper insights into the genetic determinants for histamine production in staph. capitis. | 2008 | 17887985 |
| productivity improvement of recombinant escherichia coli fermentation via robust optimization. | a nonlinear model of a recombinant escherichia coli producing porcine growth hormone (pgh) fermentation was developed. the model was used to calculate a glucose feeding and temperature strategy to optimize the production of pgh. simulations showed that the implementation of optimal feed and temperature profiles was sensitive to the maximum specific growth rate, and a mismatch could result in excessive acetate production and a significant reduction in pgh yield. an optimization algorithm was thus ... | 2008 | 17717709 |
| the effect of intramuscular administration of colistin on the development and course of experimentally induced oedema disease in weaned piglets. | shiga-toxigenic e. coli (stec) strains that produce shiga toxin stx2e cause oedema disease in weaned piglets. the purpose of the present study was to investigate the impact of stx2e released in mesenteric lymph nodes on disease pathogenesis. colistin and ampicillin were intramuscularly administered to piglets of the experimental group simultaneously challenged with stec strain, type o139:f18ab, stx2e+. piglets of the control group were challenged with stec only. the strain was naturally resistan ... | 2008 | 17977672 |
| effects of dna supercoiling and topoisomerases on the expression of genes coding for f165(1), a p-like fimbriae. | pathogenic escherichia coli 4787 (o115:kv165) causes septicemia in pigs and expresses the fimbriae f165(1) encoded by the foo operon that belongs to the p fimbrial family. fooi and foob, encoding specific foo regulators, are divergently transcribed; their intergenic region is responsible for the regulation of foo expression. the role of global and local supercoiling (transcription-induced supercoiling within the intergenic region) on the regulation of foo expression was investigated. expression ... | 2007 | 17986081 |
| dietary inclusion of colicin e1 is effective in preventing postweaning diarrhea caused by f18-positive escherichia coli in pigs. | with worldwide concern over the use of antibiotics in animal agriculture and their contribution to the spread of antibiotic resistance, alternatives to conventional antibiotics are needed. previous research in our laboratories has shown that colicin e1 is effective against some escherichia coli strains responsible for postweaning diarrhea (pwd) in vitro. in this study we examined the efficacy of the dietary inclusion of colicin e1 in preventing experimentally induced pwd caused by f18-positive e ... | 2007 | 17724148 |
| biochemical properties of an intracellular serpin from echinococcus multilocularis. | a serpin of the intracellular type from the tapeworm echinococcus multilocularis was expressed in escherichia coli, purified by ion exchange chromatography and tested for inhibitory activity against several proteinases. the recombinant protein, which after transcriptional induction, represents about 20 % of total cellular protein, is biochemically active and inhibits trypsin and the trypsin-like plasmin as well as pig pancreatic and human neutrophil elastase. implications regarding its biochemis ... | 2007 | 17727977 |
| differences in virulence among escherichia coli o157:h7 strains isolated from humans during disease outbreaks and from healthy cattle. | escherichia coli o157:h7 causes life-threatening outbreaks of diarrhea, hemorrhagic colitis, and hemolytic-uremic syndrome in humans and significant economic loss in agriculture and could be a potential agent of bioterrorism. although the prevalence of e. coli o157:h7 in cattle and other species with which humans have frequent contact is high, human infections are relatively uncommon, despite a low infectious dose. a plausible explanation for the low disease incidence is the possibility that not ... | 2007 | 17890332 |
| the effect of feeding fermented liquid whey plus dextrose inoculated with specific lactic acid bacteria of pig origin to weanling pigs challenged with escherichia coli o149:k91:f4. | the objective of this study was to determine the efficacy of using fermented liquid whey inoculated with specific lactic acid bacteria of pig origin to reduce the severity and progression of postweaning enterotoxigenic escherichia coli diarrhea in weanling pigs challenged with e. coli o149:k91:f4. based on two trials, it was determined that feeding inoculated fermented whey in a liquid diet did not affect growth performance or the severity or duration of postweaning diarrhea compared with a conv ... | 2007 | 17926306 |
| antimicrobial susceptibility of escherichia coli from swine, horses, dogs and cats as determined in the bft-germvet monitoring program 2004-2006. | a total of 417 isolates of escherichia coli collected from five animal species/organ system combinations from swine [urinary/genital tract (ugt) incl. mastitis metritis agalactia syndrome], horses [genital tract (gt)] and dogs/cats [respiratory tract (rt), ugt and gastrointestinal tract (git)] were analysed quantitatively for their susceptibility against different antimicrobial agents by determination of minimum inhibitory concentrations. regardless of which animal species the strains originated ... | 2007 | 17939454 |
| composition of intestinal enterobacteriaceae populations of healthy domestic pigs. | in this study, the enterobacteriaceae microbiota, including their diversity as well as the distribution of haemolytic and virulence gene-harbouring escherichia coli of 56-day-old healthy piglets, was characterized. both the composition and the diversity of enterobacteriaceae populations varied considerably between individual pigs and intestinal sections. e. coli, enterobacter cloacae, citrobacter freundii and klebsiella pneumoniae dominated the enterobacteriaceae microbiota. however, mucosa-asso ... | 2007 | 17975092 |
| adult hemolytic-uremic syndrome associated with urosepsis due to shigatoxin-producing escherichia coli o138:h-. | we report the case of a 62-year-old man without prodromal symptoms who developed a hemolytic-uremic syndrome (hus) one week after the diagnosis of an acute bacteremic urinary infection (uti). in this patient, the e. coli isolated in blood cultures was a non-o157:h7 shigatoxin-producing strain that could subsequently be identified as o138:h-. this is a strain that is normally found in pigs and that has never been isolated in humans previously. uti-related hus is a rare event, as until now, only 1 ... | 2007 | 17763172 |
| enterocyte proliferation and apoptosis in the caudal small intestine is influenced by the composition of colonizing commensal bacteria in the neonatal gnotobiotic pig. | we previously reported marked differences in small intestinal morphology, including changes in crypt depth and villous height, after inoculation of germ-free pigs with different bacterial species. in an attempt to identify the mechanisms governing changes in villous morphology associated with bacterial colonization, 2 gnotobiotic experiments were performed. in each experiment, 16 piglets were allocated to 4 treatment groups including germ-free (gf), monoassociation with lactobacillus fermentum ( ... | 2007 | 17785595 |
| [analysis of genetic variations at m857 locus of the a1-fucosy- trans-ferase (fut1) orf in pigs]. | enterotoxigenic escherichia coli f18 (etec f18) is the main pathogen that causes edema disease and post-weaning diarrhea in piglets, and a1-fucosytransferase (fut1) gene has been identified as a receptor gene encoding the receptor for etec f18 bacteria. in this study, the method of pcr-rflp was used to investigate the among 21 breeds including one wild boar breed and 20 western commercial and chinese native pig breeds (populations). the results showed that none of the individuals in all 21 breed ... | 2007 | 17855256 |
| characterization of polymorphisms of transferrin receptor and their association with susceptibility to etec f4ab/ac in pigs. | diarrhoea caused by enterotoxigenic escherichia coli (etec) expressing f4 (f4ab, f4ac and f4ad) fimbriae is a significant cause of mortality and morbidity in newborn and weaned pigs. the locus controlling susceptibility towards etec f4ab/ac has been mapped to ssc13q41, in which tfrc (transferrin receptor) was localized and considered as a positional candidate gene for etec f4ab/ac receptor. in this study, we determined susceptibility/resistance to etec f4ab/ac in a total of 755 f2 animals from a ... | 2007 | 17651325 |
| reservoirs of antimicrobial resistance in pet animals. | increasing amounts of antimicrobials are used in pets, including substances used in human medicine (in particular, broad-spectrum agents such as clavulante-potentiated aminopenicillins, cephalosporins, and fluoroquinolones). there is evidence that resistance to antimicrobials is growing among bacteria causing infection in pets. these bacteria include staphylococcus intermedius and escherichia coli, as well as other organisms of clinical importance in humans, including methicillin-resistant staph ... | 2007 | 17683019 |
| influence of endotoxin-induced sepsis on the requirements of propofol-fentanyl infusion rate in pigs. | endotoxin-induced sepsis in pigs is a recognized experimental model for the study of human septic shock. generally, pigs are brought into general anaesthesia before sepsis is induced. it is our experience that drug dosages of propofol and fentanyl need to be reduced during endotoxin-induced sepsis, in order to prevent respiratory and cardiovascular depression, but the scientific evidence for this observation is lacking. therefore, we measured the consumption of propofol and fentanyl at equal lev ... | 2007 | 17697040 |
| the role of secondary structure in the entropically driven amelogenin self-assembly. | amelogenin, the major extracellular enamel matrix protein, plays critical roles in controlling enamel mineralization. this generally hydrophobic protein self-assembles to form nanosphere structures under certain solution conditions. to gain clearer insight into the mechanisms of amelogenin self-assembly, we first investigated the occurrences of secondary structures within its sequence. by applying isothermal titration calorimetry (itc), we determined the thermodynamic parameters associated with ... | 2007 | 17704165 |
| high-level prokaryotic expression of envelope exterior of membrane protein of porcine epidemic diarrhea virus. | the truncated fragment m' gene, encoding the exterior of the viral envelope protein of pedv, was subcloned into prokaryotic expression vector pgex-6p-1. the recombinant plasmid pgex-6p-m' was constructed and transformed into e. coli bl21(de3)plyss for expression. sds-page analysis showed recombinant truncated m' protein was highly expressed by pgex-6p-m' and the product fusion protein gst-m' reached 45% in the total bacteria proteins with the analysis of software alphaimager2200. the preliminary ... | 2007 | 17475420 |
| development of intracellular bacterial communities of uropathogenic escherichia coli depends on type 1 pili. | uropathogenic escherichia coli, the predominant causative agent of urinary tract infections, use type 1 pili to bind and invade bladder epithelial cells. upon entry, the bacteria rapidly replicate and enter a complex developmental pathway ultimately forming intracellular bacterial communities (ibcs), a niche with biofilm-like properties protected from innate defences and antibiotics. paradoxically, bacteria within ibcs produce type 1 pili, an organelle thought only to be an extracellular coloniz ... | 2007 | 17490405 |
| cardiovascular effects of levosimendan in the early stages of endotoxemia. | sepsis-associated myocardial depression is associated with calcium desensitization and adrenergic uncoupling. we conducted a prospective randomized investigation on the effects of the calcium sensitizer, levosimendan, on hemodynamics, myocardial blood flow, and myocardial lactate metabolism during porcine endotoxemia. twelve pigs were studied. oxygen consumption was measured by indirect calorimetry, and myocardial blood flow was measured by retrograde thermodilution. pulmonary, arterial, and ven ... | 2007 | 17510599 |
| regulation of the muscle-specific amp-activated protein kinase alpha2beta2gamma3 complexes by amp and implications of the mutations in the gamma3-subunit for the amp dependence of the enzyme. | the amp-activated protein kinase is an evolutionarily conserved heterotrimer that is important for metabolic sensing in all eukaryotes. the muscle-specific isoform of the regulatory gamma-subunit of the kinase, amp-activated protein kinase gamma3, has a key role in glucose and fat metabolism in skeletal muscle, as suggested by metabolic characterization of humans, pigs and mice harboring substitutions in the amp-binding bateman domains of gamma3. we demonstrate that amp-activated protein kinase ... | 2007 | 17518971 |
| rapid and widespread dissemination of multidrug-resistant blacmy-2 salmonella typhimurium in mexico. | we describe the emergence and dissemination of multidrug-resistant (mdr) salmonella typhimurium in humans, retail meat and food animals from yucatan, mexico. | 2007 | 17526503 |
| a survey of the microbiological quality of kangaroo carcasses processed for human consumption in two processing plants in queensland, australia. | an investigation of the microbiological quality of kangaroo carcasses at two queensland processing plants was carried out. a total of 836 whole muscle samples were taken, 801 from plant a and 35 from plant b. samples were analyzed for aerobic bacteria, escherichia coli, and salmonella. the mean adjusted aerobic plate count (apc) was 2.8 log cfu/g, and counts at the 90th, 95th, and 99th percentiles were 4.2, 4.9, and 6.4 log cfu/g, respectively. the maximum number of bacteria recovered was 6.5 lo ... | 2007 | 17536688 |
| risk factors for antimicrobial resistance among fecal escherichia coli from residents on forty-three swine farms. | fecal escherichia coli (n = 555) were isolated from 115 residents on 43 farrow-to-finish swine farms to determine the prevalence of antimicrobial resistance and associated risk factors. susceptibility to 21 antimicrobials was determined and the overall prevalence of antimicrobial resistance was 25.8%. pair-wise difference in prevalences of resistance to individual antimicrobials was significant between isolates from residents on farms that fed medicated swine rations compared to those that did n ... | 2007 | 17536936 |
| reliability of continuous pulse contour cardiac output measurement during hemodynamic instability. | arterial pulse contour analysis is gaining widespread acceptance as a monitor of continuous cardiac output (co). while this type of co measurement is thought to provide acceptable continuous measurements, only a few studies have tested its accuracy and repeatability under unstable hemodynamic conditions. we compared continuous co measurement using the pulse contour method (pcco) before and after calibration with intermittent transpulmonary thermodilution cardiac output (tpco). | 2007 | 17541714 |
| effects of bacterial colonization on the porcine intestinal proteome. | the gastrointestinal tract harbors a complex community of bacteria, of which many may be beneficial. studies of germ-free animal models have shown that the gastrointestinal microbiota not only assists in making nutrients available for the host but also contributes to intestinal health and development. we studied small intestinal protein expression patterns in gnotobiotic pigs maintained germ-free, or monoassociated with either lactobacillus fermentum or non-pathogenic escherichia coli. a common ... | 2007 | 17542629 |
| different probiotic properties for lactobacillus fermentum strains isolated from swine and poultry. | systematic procedures were used to evaluate the probiotic properties of lactobacillus fermentum (l. fermentum) strains isolated from swine and poultry. the major properties included their capabilities to adhere to the intestinal epithelium of swine and poultry, the inhibition on pathogenic bacteria, and their tolerance to the gastric juice and bile salts. results showed that l. fermentum strains from poultry digestive tract showed better adherence to the swine intestine and chicken crop epitheli ... | 2007 | 17544731 |
| evaluation of two library-independent microbial source tracking methods to identify sources of fecal contamination in french estuaries. | in order to identify the origin of the fecal contamination observed in french estuaries, two library-independent microbial source tracking (mst) methods were selected: (i) bacteroidales host-specific 16s rrna gene markers and (ii) f-specific rna bacteriophage genotyping. the specificity of the bacteroidales markers was evaluated on human and animal (bovine, pig, sheep, and bird) feces. two human-specific markers (hf183 and hf134), one ruminant-specific marker (cf193'), and one pig-specific marke ... | 2007 | 17557850 |
| long-term changes in hepatobiliary physiology after roux-en-y hepaticojejunostomy. | hepaticojejunostomy (hj) is a common operation used to by-pass extrahepatic biliary obstructions and to establish biliary-enteric continuity after resections for benign and malignant diseases. little is known about the effect of this procedure on hepatobiliary physiology. the aim of the present study was to investigate in a swine model the changes in biliary dynamics, bile composition, and hepatic histology induced by roux-y hj. twenty-four swine (57 (47 to 76) kg) underwent cholecystectomy, wit ... | 2007 | 17574593 |
| contribution of multi-antimicrobial resistance to the population of antimicrobial resistant escherichia coli isolated from apparently healthy pigs in japan. | the aim of this study was to clarify the involvement of tetracycline usage in resistance rates against other antimicrobials. antimicrobial susceptibility testing was carried out on 545 porcine escherichia coli isolates throughout japan. as the result of analyzing by regions, resistance rates against kanamycin, oxytetracycline and trimethoprim in the kanto/koshinetu district were higher than those in some other districts. high resistance rates against kanamycin or trimethoprim in oxytetracycline- ... | 2007 | 17579258 |
| testing of swine feces obtained through the national animal health monitoring system's swine 2000 study for the presence of escherichia coli o157:h7. | fecal samples collected from healthy pigs from 13 of the top 17 swine-producing states were tested for escherichia coli o157:h7 as part of the national animal health monitoring system swine 2000 study. serogroup o157 strains were isolated from 106 of 2,526 fecal samples. none of the isolates were positive by pcr for the flich7 (h7 flagellin) gene or for the hly933 (hemolysin) gene; however, one isolate was positive for the stxl gene (shiga toxin 1), an additional four isolates were positive for ... | 2007 | 17612081 |
| temporal dynamics and impact of manure storage on antibiotic resistance patterns and population structure of escherichia coli isolates from a commercial swine farm. | many confined-livestock farms store their wastes for several months prior to use as a fertilizer. storing manure for extended periods could significantly bias the composition of enteric bacterial populations subsequently released into the environment. here, we compared populations of escherichia coli isolated from fresh feces and from the manure-holding tank (stored manure) of a commercial swine farm, each sampled monthly for 6 months. the 4,668 confirmed e. coli isolates were evaluated for resi ... | 2007 | 17616622 |
| prevalence and pattern of antimicrobial susceptibility in escherichia coli isolated from pigs reared under antimicrobial-free and conventional production methods. | to determine and compare levels and patterns of antimicrobial resistance among escherichia coli isolated from pigs on farms that did not use antimicrobial agents versus pigs produced under conventional methods. | 2007 | 17630898 |
| relationship between o-antigen subtypes, bacterial surface structures and o-antigen gene clusters in escherichia coli o123 strains carrying genes for shiga toxins and intimin. | escherichia coli o123 strains express a broad spectrum of phenotypes, h serotypes and virulence markers and are able to colonize and to cause disease in different hosts including humans. in this study, two subtypes of e. coli o123 antigen (group i and group ii) have been identified based on their cross-reactions with other e. coli o antigens. investigation of the relationship between o123 group i and group ii strains by o serotyping and fourier transform infrared spectroscopy of whole bacteria r ... | 2007 | 17244797 |
| mechanical ventilation and lung infection in the genesis of air-space enlargement. | air-space enlargement may result from mechanical ventilation and/or lung infection. the aim of this study was to assess how mechanical ventilation and lung infection influence the genesis of bronchiolar and alveolar distention. | 2007 | 17274806 |
| non-toxic pseudomonas aeruginosa exotoxin a expressing the fmdv vp1 g-h loop for mucosal vaccination of swine against foot and mouth disease virus. | synthetic peptides derived from the g-h loop of the foot and mouth disease virus (fmdv) capsid protein vp1 are relatively poor at recapitulating the native conformation present in the virus, and thus are often poor immunogens. we hypothesized that a candidate mucosal vaccine against fmdv could be developed using the non-toxic pseudomonas aeruginosa exotoxin a (ntpe) to deliver the g-h loop in its native conformation. an added benefit of this approach is the potential for ntpe to serve as an effe ... | 2007 | 17276557 |
| expression, purification, crystallization and preliminary x-ray diffraction analysis of the vp8* sialic acid-binding domain of porcine rotavirus strain osu. | the rotavirus outer capsid spike protein vp4 is utilized in the process of rotavirus attachment to and membrane penetration of host cells. vp4 is cleaved by trypsin into two domains: vp8* and vp5*. the vp8* domain is implicated in initial interaction with sialic acid-containing cell-surface carbohydrates and triggers subsequent virus invasion. the vp8* domain from porcine osu rotavirus was cloned and expressed in escherichia coli. different crystal forms (orthorhombic p2(1)2(1)2(1) and tetragona ... | 2007 | 17277447 |
| widespread distribution of tetracycline resistance genes in a confined animal feeding facility. | we sought to determine the distribution of resistance and the tetracycline resistance genes among bacteria isolated from a swine confined animal feeding facility where tetracycline-containing feed had been in use for over 20 years. samples collected from feed, hogs, hog houses, waste lagoon, soil, surface water and well water were screened for the presence of (a) resistant escherichia coli and enterococci and (b) tetracycline-resistant strains of all species. genomic dna was extracted from the l ... | 2007 | 17287111 |
| effects of water and diet acidification with and without antibiotics on weanling pig growth and microbial shedding. | two 5-wk experiments were conducted to determine the effects of water and diet acidification with and without antibiotics on weanling pig growth performance and microbial shedding. in exp. 1, 204 pigs (19.2 d of age) were used in a 3 x 2 factorial, with 3 dietary treatments fed with or without water acidification (2.58 ml/l of a propionic acid blend; kem san, kemin americas, des moines, ia). dietary treatments were: 1) control, 2) control + 55 ppm of carbadox (cb), and 3) dietary acid [da; contr ... | 2007 | 17296768 |
| structures of activated fructose-1,6-bisphosphatase from escherichia coli. coordinate regulation of bacterial metabolism and the conservation of the r-state. | the enteric bacterium escherichia coli requires fructose-1,6-bisphosphatase (fbpase) for growth on gluconeogenic carbon sources. constitutive expression of fbpase and fructose-6-phosphate-1-kinase coupled with the absence of futile cycling implies an undetermined mechanism of coordinate regulation involving both enzymes. tricarboxylic acids and phosphorylated three-carbon carboxylic acids, all intermediates of glycolysis and the tricarboxylic acid cycle, are shown here to activate e. coli fbpase ... | 2007 | 17314096 |
| comparison of immune responses in parenteral faeg dna primed pigs boosted orally with f4 protein or reimmunized with the dna vaccine. | we previously showed that an intradermal (i.d.) faeg dna prime (2x)-oral f4 protein boost immunization induces a systemic response and weakly primes a mucosal igg response in pigs, especially when plasmid vectors encoding the a and b subunit of the e. coli thermo-labile enterotoxin (lt) are added to the dna vaccine. in the present study, we evaluated whether addition of 1alpha,25-dihydroxyvitamin d(3) (vitd(3)) to the dna vaccine could further enhance this mucosal priming and/or modulate the ant ... | 2007 | 17331591 |
| tile water quality following liquid swine manure application into standing corn. | the quality of water draining fields fertilized with liquid swine (sus scrofa) manure (lsm) sidedressed into standing corn (zea mays l.) at rates ranging from 0 to 94 m(3) ha(-1), either topdressed (td) onto the surface, or injected (inj) into the soil once annually for each of three consecutive years was evaluated. liquid swine manure application rate was a critical driver of preferential flow of lsm to tile as detected by turbidity, concentrations of nh(4)(+)-n, dissolved reactive phosphorus ( ... | 2007 | 17332262 |
| msg1, a surface-localised protein of mycoplasma suis is involved in the adhesion to erythrocytes. | mycoplasma suis is a member of the group of uncultivable haemoplasmas which colonise erythrocytes of a wide range of vertebrates. adhesion to erythrocytes is the crucial step in the unique haemoplasma life cycle. due to the lack of a cultivation system, no adhesion structures have been identified so far. in order to determine potential adhesion molecules of m. suis, we screened genomic m. suis libraries. the protein msg1 with glyceraldehyde-3-phosphate dehydrogenase (gapdh) similarity was identi ... | 2007 | 17336568 |
| two specific sites for binding of k88ab escherichia coli fimbriae to porcine intestinal brush border membranes. | we have studied the characteristics of the binding of the k88ab escherichia coli fimbrial antigen to porcine brush border membranes by solid phase binding assay. binding of biotinylated k88ab to brush border membranes followed a sigmoidal dependence and was saturable, apparent saturation occurring with 0.8 ng of fimbriae (approx. 7 ng of fimbriae per microg of brush border protein) irrespective of incubation temperature in the range of room temperature to 4 degrees c. a hill plot of log [(fimbri ... | 2007 | 17337055 |
| distribution of supplemental escherichia coli appa2 phytase activity in digesta of various gastrointestinal segments of young pigs. | the objective of this study was to determine the functional location and disappearance of activity of a supplemental escherichia coli appa2 phytase and its impact on digesta p and ca concentrations in the gastrointestinal tract of pigs. in exp. 1, 18 pigs (8.3 +/- 0.2 kg of bw) were allotted to 3 groups (n = 6 each) and fed a low-p (0.4%) corn-soybean meal, basal diet (bd), bd + phytase [500 units (u)/kg of feed], or bd + inorganic p (ip, 0.1%) for 4 wk. in exp. 2, 30 pigs (14.5 +/- 0.2 kg of bw ... | 2007 | 17339410 |
| emergence of rmtb methylase-producing escherichia coli and enterobacter cloacae isolates from pigs in china. | to investigate the occurrence of 16s rrna methylases conferring high-level resistance to aminoglycosides in enterobacteriaceae isolated from two pig farms in china. | 2007 | 17353219 |
| antimicrobial resistance observed in escherichia coli strains isolated from fecal samples of cattle and pigs in korea during 2003-2004. | a total of 744 escherichia coli strains isolated from 830 fecal samples of healthy cattle and pigs in all provinces of korea were examined for resistance to 16 antimicrobials. the most frequently observed resistance in cattle isolates was to tetracycline (30.5%), followed by resistance to streptomycin (20.4%), ampicillin (12.0%) and chlorampenicol (6.9%). prevalences of resistance to the same four antimicrobials in swine isolates were 96.3%, 66.8%, 66.1%, and 47.6%, respectively. the prevalence ... | 2007 | 17363100 |
| prevalence of virulence genes in escherichia coli strains recently isolated from young pigs with diarrhea in the us. | enterotoxigenic escherichia coli (etec)-associated post-weaning diarrhea (pwd) is economically one of the most important diseases for the swine industry. porcine etec strains typically express k88 or f18 fimbria and heat-labile (lt) and/or heat-stable (sta, stb) enterotoxins. however, recent studies indicate that east1 toxin, adhesin involved in diffuse adherence (aida-i) and porcine attaching and effacing-associated factor (paa) may also be expressed by etec strains associated with diarrhea. to ... | 2007 | 17368762 |
| development and evaluation of enzyme-linked immunosorbent assay based on recombinant nucleocapsid protein for detection of porcine epidemic diarrhea (pedv) antibodies. | an enzyme-linked immunosorbent assays (elisa) based on recombinant nucleocapsid (n) protein generated in escherichia coli was evaluated for its sensitivity and specificity for diagnosis of porcine epidemic diarrhea (pedv) infection. the n gene encoding the n protein was cloned and expressed as a fusion protein with his tag protein in e. coli. the recombinant n protein was migrated at 48 kda and reacted with six histidine tag specific monoclonal antibody by immunoblotting. recombinant n protein e ... | 2007 | 17368968 |
| antimicrobial treatment reduces intestinal microflora and improves protein digestive capacity without changes in villous structure in weanling pigs. | the immediate post-weaning period is often associated with gut malfunction and diarrhoea for young pigs. administration of antimicrobials remains an effective way to control weaning diarrhoea but it remains unclear how they affect gut physiology and microbiology although this is a prerequisite for being able to devise better alternatives. hence, for 7 d we treated pigs, weaned at 24 d of age, with a combination of amoxicillin (25 mg/kg feed and injection of 8.75 mg/kg body weight per 12 h) and z ... | 2007 | 17381960 |
| quantitation of 17beta-nandrolone metabolites in boar and horse urine by gas chromatography-mass spectrometry. | a method to quantify metabolites of 17beta-nandrolone (17betan) in boar and horse urine has been optimized and validated. metabolites excreted in free form were extracted at ph 9.5 with tert-butylmethylether. the aqueous phases were applied to sep pak c18 cartridges and conjugated steroids were eluted with methanol. after evaporation to dryness, either enzymatic hydrolysis with beta-glucuronidase from escherichia coli or solvolysis with a mixture of ethylacetate:methanol:concentrated sulphuric a ... | 2007 | 17386711 |
| screening of pigs resistant to f4 enterotoxigenic escherichia coli (etec) infection. | the present study analysed quantitatively the mucin 4 polymorphism for determining the f4ac/ab receptor status of a total of 63 pigs by comparing it with the in vitro villous adhesion assay. the probability of a susceptible genotype for the mucin 4 increases significantly with increasing f4ab or f4ac etec adhesion per 250 microm villi (p=0.029 for f4ab, p=0.030 for f4ac), with the odds ratio for each unit increase of f4ab or f4ac equal to, respectively, 1.036 (95% ci [1.004-1.069]) and 1.018 (95 ... | 2007 | 17399917 |
| cardiac effects of endothelin receptor antagonism in endotoxemic pigs. | myocardial depression in sepsis is frequently encountered clinically and contributes to morbidity and mortality. increased plasma levels of endothelin-1 (et-1) have been described in septic shock, and previous reports have shown beneficial effects on cardiovascular performance and survival in septic models using et receptor antagonists. the aim of the current study was to investigate specific cardiac effects of et receptor antagonism in endotoxicosis. sixteen domestic pigs were anesthetized and ... | 2007 | 17400719 |
| detection of bacteroidales fecal indicators and the zoonotic pathogens e. coli 0157:h7, salmonella, and campylobacter in river water. | bacteroidales host-specific pcr offers a rapid method of diagnosing fecal pollution in water and identifying sources of input. to assess human health risks from exposure to fecal pathogens, however, bacteroidales markers should be detectable when pathogens are present. to determine if bacteroidales general, human-, ruminant-, and swine-specific markers correlate with certain fecal pathogens, we conducted a retrospective study on water samples for which the presence of e. coli o157:h7, salmonella ... | 2007 | 17410775 |
| crystal structures of a poxviral glutaredoxin in the oxidized and reduced states show redox-correlated structural changes. | glutaredoxins act as reducing agents for the large subunit of ribonucleotide reductase (r1) in many prokaryotes and eukaryotes, including humans. the same relationship has been proposed for the glutaredoxin and r1 proteins expressed by all orthopoxviruses, including vaccinia, variola, and ectromelia virus. interestingly, the orthopoxviral proteins share 45% and 78% sequence identity with human glutaredoxin-1 (grx-1) and r1, respectively. to study structure-function relationships of the vertebrat ... | 2007 | 17137595 |
| expression of porcine circovirus 2 orf2 gene requires codon optimized e. coli cells. | expression and purification of whole and nuclear localization signal (nls) deleted orf2 capsid protein of porcine circovirus 2 (pcv2) is demonstrated in the present study. gene coding for both protein forms were cloned into pdest17 vector and expressed in bl21 (de3)ai cells and in bl21-codonplus (de3)-ripl e. coli cells. the later cells were used to overcome difficulties with the heterologous expression of viral proteins in prokaryotic systems. whole 30 kda recombinant orf2 protein was successfu ... | 2007 | 17139551 |
| classification of temporal profiles of f4+ e. coli shedding and faecal dry matter in experimental post-weaning diarrhoea of pigs. | enterotoxigenic f4+ escherichia coli can colonize the intestine of pigs and cause diarrhoea. our primary goal was to find a discriminant rule to discriminate between f4+ e. coli shedding profiles as this may reflect differences in the infectiousness of pigs. our secondary goal was to find a discriminant rule to discriminate between diarrhoeic and non-diarrhoeic pigs. repeated measurements (bacterial shedding and percentage dry matter of faeces) were taken of 74 weaned pigs that were infected exp ... | 2007 | 17156498 |
| phenotypic characterization of escherichia coli through whole-cell fatty acid profiling to investigate host specificity. | the objective of the study was to investigate whole-cell fatty acid methyl ester (fame) profiles of 605 escherichia coli isolates to determine their host specificity. the isolates were cultured from six possible sources of fecal pollution; 180 isolates from sewage, 85 from dairy cow, 98 from chicken, 76 from swine, 94 from deer, and 72 from waterfowl, mostly geese and ducks. the fame profiles were presented as the relative masses of 12 fames identified in the isolates and it was found that none ... | 2007 | 17234236 |
| functional and structural characterization of d-aspartate oxidase from porcine kidney: non-michaelis kinetics due to substrate activation. | d-aspartate oxidase (ddo, ec 1.4.3.1) catalyzes dehydrogenation of d-aspartate to iminoaspartate and the subsequent re-oxidation of reduced fad with o2 to produce hydrogen peroxide. in the mammalian neuroendocrine system, d-aspartate, a natural substrate, plays important roles in the regulation of the synthesis and secretion of hormones. to elucidate the kinetic and structural properties of native ddo, we purified ddo from porcine kidney to homogeneity, cloned the cdna, and overexpressed the enz ... | 2007 | 17234685 |
| effects of acid lac and kem-gest acid blends on growth performance and microbial shedding in weanling pigs. | weanling pigs with mean initial bw of 6.04 kg (exp.1) and 5.65 kg (exp. 2) and mean age at weaning of 18.2 d (exp. 1) and 17.7 d (exp. 2) were used in two 5-wk experiments (exp. 1, n = 180; exp. 2, n = 300) to evaluate the effects of an organic acid blend (acid lac, kemin americas inc., des moines, ia) and an inorganic/organic acid blend (kem-gest, kemin americas inc.) on weanling pig growth performance and microbial shedding. in exp. 1, the 5 dietary treatments were 1) negative control, 2) diet ... | 2007 | 17235031 |
| proinflammatory cytokine mrna expression in mammary tissue of sows following intramammary inoculation with escherichia coli. | the expression of the proinflammatory cytokines interleukin-1beta (il-1beta), il-6, il-8, and tumor necrosis factor-alpha (tnf-alpha) mrnas was determined by reverse transcription-pcr (rt-pcr) analysis of biopsies from the mammary glands of sows. the biopsies were collected before and 24h after intramammary inoculation of 12 pregnant sows with escherichia coli (e. coli). four of the sows developed clinical signs of mastitis and these animals displayed significantly lower levels of il-1beta mrna ... | 2007 | 17215047 |
| identification and characterization of the ntpase activity of classical swine fever virus (csfv) nonstructural protein 3 (ns3) expressed in bacteria. | the nonstructural protein 3 (ns3) of members of the family flaviviridae possesses multiple enzyme activities that are likely to be essential for viral replication. here, we cloned and expressed full-length csfv ns3 protein (ns3fl) and its n-terminal truncated version (ntns3) in e. coli. ntpase activities of the purified ns3fl and ntns3 proteins and their reaction conditions were investigated. the results showed that csfv ns3fl and ntns3 proteins contained a specific polynucleotide-stimulated ntp ... | 2007 | 17447110 |
| dna microarray-based identification of serogroups and virulence gene patterns of escherichia coli isolates associated with porcine postweaning diarrhea and edema disease. | escherichia coli strains causing postweaning diarrhea (pwd) and edema disease (ed) in pigs are limited to a number of serogroups, with o8, o45, o138, o139, o141, o147, o149, and o157 being the most commonly reported worldwide. in this study, a dna microarray based on the o-antigen-specific genes of all 8 e. coli serogroups, as well as 11 genes encoding adhesion factors and exotoxins associated with pwd and ed, was developed for the identification of related serogroups and virulence gene patterns ... | 2007 | 17449692 |
| the non-o157 shiga-toxigenic (verocytotoxigenic) escherichia coli; under-rated pathogens. | following a brief review of the ecology of escherichia coli in general, the role of shiga-toxigenic (verocytotoxigenic) e. coli (stec) as pathogens is addressed. while stec belonging to the serogroup o157 have been extensively studied and shown to be involved in many cases and outbreaks of human disease, the importance of stec belonging to other serogroups has not been recognized as much. this review addresses the problems associated with these pathogens, demonstrating that increasing the awaren ... | 2007 | 17453930 |
| study of the efficacy of coated vicryl plus antibacterial suture (coated polyglactin 910 suture with triclosan) in two animal models of general surgery. | to evaluate the efficacy in vitro and in vivo of a new antibacterial suture (pgab) compared with a traditional braided suture (pg). our primary goals were to study microbiological effectiveness and impact on wound healing of pgab vs pg. secondary goal was to analyze influence on inflammatory response. | 2007 | 16487594 |
| survival of escherichia coli o157:h7 in dry fermented sausages containing micro-encapsulated probiotic lactic acid bacteria. | escherichia coli o157:h7 is capable of surviving the rigorous processing steps during the manufacture of dry fermented sausages. the effect of adding two probiotic organisms, lactobacillus reuteri and bifidobacterium longum as co-cultures with the meat starter cultures pediococcus pentosaceus and staphylococcus carnosus on the viability of e. coli o157:h7 in dry fermented sausages was studied. a 5 strain cocktail of e. coli o157:h7 was added at 7.4 log cfu/g to the sausage batter and challenged ... | 2007 | 16943098 |
| effects of crude red kidney bean lectin (phytohemagglutinin) exposure on performance, health, feeding behavior, and gut maturation of pigs at weaning. | the aim of this study was to obtain information that could help to ease the weaning transition in commercial pig production. before weaning, phytohemagglutinin (pha) in the form of a crude preparation of red kidney bean lectin was fed by gavage to 24 crossbred [(swedish landrace x yorkshire) x hampshire] piglets, whereas 24 control piglets were fed alpha-lactalbumin by gavage, to study the effect on growth, occurrence of postweaning diarrhea, feeding behavior, and some anatomical and physiologic ... | 2007 | 17040948 |
| identification of the actinobacillus pleuropneumoniae leucine-responsive regulatory protein and its involvement in the regulation of in vivo-induced genes. | actinobacillus pleuropneumoniae is a gram-negative bacterial pathogen that causes a severe hemorrhagic pneumonia in swine. we have previously shown that the limitation of branched-chain amino acids (bcaas) is a cue that induces the expression of a subset of a. pleuropneumoniae genes identified as specifically induced during infection of the natural host animal by using an in vivo expression technology screen. leucine-responsive regulatory protein (lrp) is a global regulator and has been shown in ... | 2007 | 17060463 |
| cdna cloning and overexpression of acidic ribosomal phosphoprotein p1 gene (rplp1) from the giant panda. | rplp1 is one of acidic ribosomal phosphoproteins encoded by rplp1 gene, which plays an important role in the elongation step of protein synthesis. the cdna of rplp1 was cloned successfully for the first time from the giant panda (ailuropoda melanoleuca) using rt-pcr technology, which was also sequenced, analyzed preliminarily and expressed in e.coli. the cdna fragment cloned is 449bp in size, containing an open reading frame of 344bp encoding 114 amino acids. alignment analysis indicated that th ... | 2007 | 18071584 |
| gene cloning, expression, and functional characterization of an ornithine decarboxylase protein from serratia liquefaciens ifi65. | putrescine has a negative effect on health and is also used as an indicator of quality on meat products. we investigated the genes involved in putrescine production by serratia liquefaciens ifi65 isolated from a spoiled spanish dry-cured ham. we report here the genetic organization of its ornithine decarboxylase encoding region. the 5506-bp dna region showed the presence of three complete and two partial open reading frames. putative functions have been assigned to several gene products by seque ... | 2007 | 18050943 |
| [cloning and expression of f18 fimbrial operon gene clusters from enterotoxigenic escherichia coli and their bioactivity]. | the fed operon gene clusters with each size of 5.6kb, encoding the f18ab or f18ac fimbriae, was amplified respectively by high fidelity pcr using the genomic dna templates from f18 fimbriae e. coli strains 107/86 or 2134p. the pcr products with the restriction enzyme sites at each end were digested and then cloned into the vector pet-22b (+), the recombinant plamids with the inserts of both type of fed gene clusters were constructed and screened, further confirmed by the means of combination wit ... | 2007 | 18062250 |
| evidence for neuromodulation of enteropathogen invasion in the intestinal mucosa. | the extensively innervated intestinal mucosa encompasses a vast surface exposed to an array of potentially infectious microorganisms. we investigated the role of enteric nerves in modulating intracellular internalization of a multidrug-resistant salmonella typhimurium dt104 field isolate in mucosa-submucosa sheets from the porcine ileum, a biomedical model for the human intestine. the effects of transmural electrical stimulation and drugs on intracellular internalization of salmonella over 90 mi ... | 2007 | 18040851 |
| [fusion expression of slt-iieb gene and fedf gene of ee in escherichia coli and its immunogenicity]. | the dna fragment encoding the truncated slt-iieb and fedf of ee strain were fused to the downstream of glutathione s-transferase (gst) of pgex-kg expression vector, resulting in the fusion expression plasmid pksf. after transformed into e. coli bl21 (de3) and induced by iptg, the results of sds-page showed that the gst-sf fusion protein was expressed in high level. western blot was performed to confirm that the expressed fusion protein could specifically react with mouse anti-slt-iieb antiserum, ... | 2007 | 18271261 |
| effects of the administration of lactobacilli, maltodextrins and fructooligosaccharides upon the adhesion of e. coli o8:k88 to the intestinal mucosa and organic acid levels in the gut contents of piglets. | the influence of the administration of lactobacillus plantarum, maltodextrin maldex 150 and raftifeed ipx fructooligosaccharides on the inhibition of adhesion of e. coli o8:k88 to the mucosa of the jejunum, ileum and colon as well as on the organic acid levels was investigated in 33 conventional piglets. the counts of e. coli k88 adhering to the jejunal mucosa were significantly decreased (p < 0.05) in lact. plantarum + maldex 150 and lact. plantarum + maldex 150 + raftifeed ipx groups. the coun ... | 2007 | 18217248 |