Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
circulating human antibody-secreting cells during vaccinations and respiratory viral infections are characterized by high specificity and lack of bystander effect.surges of serum abs after immunization and infection are highly specific for the offending ag, and recent studies demonstrate that vaccines induce transient increases in circulating ab-secreting cells (ascs). these ascs are highly enriched but not universally specific for the immunizing ag, suggesting that a fraction of these ascs could arise from polyclonal bystander stimulation of preexisting memory cells to unrelated ags. this model is proposed to explain maintenance of long-lived serological ...201121441455
role and predictive strength of transglutaminase type 2 expression in premalignant lesions of the cervix.the demonstration that type 2 transglutaminase (tg2) can incorporate polyamine into the e7 oncoprotein of human papillomavirus (hpv) type 18 has led to the hypothesis that tg2 can have a role in the host cellular response to hpv infection. the aim of this study was to investigate whether hpv-related pathology, in infected human cervical epithelium, was associated with modulation of tg2 expression. normal controls and hpv-infected cervical biopsies were analyzed for the expression of tg2, and the ...201121441900
p16/ki-67 dual-stain cytology in the triage of ascus and lsil papanicolaou cytology: results from the european equivocal or mildly abnormal papanicolaou cytology study.the objective of this study was to analyze the diagnostic performance of a newly established immunocytochemical dual-stain protocol, which simultaneously detects p16(ink4a) and ki-67 expression in cervical cytology samples, for identifying high-grade cervical intraepithelial neoplasia (cin2+) in women with papanicolaou (pap) cytology results categorized as atypical squamous cells of undetermined significance (ascus) or low-grade squamous intraepithelial lesions (lsil).201121442767
human papillomavirus genotyping as a predictor of high-grade cervical dysplasia in women with mildly cytologic abnormalities: a two-year follow-up report.high-risk human papillomavirus (hr-hpv) dna testing has emerged as another testing modality for women with mildly cytologic abnormalities. we conducted a two-year follow-up study of 108 women with mildly abnormal cervical cytology for detection of cin 2/3. a cervical swab sample was obtained for hpv genotyping by a hpv blot and histologic follow-up results were correlated with hr-hpv types. of the 108 cases, 93 (86.1%) were positive for hr-hpv dna. hpv-16 was detected in 45.1% of patients. cin g ...201121442773
the role of human papillomavirus in the pathogenesis of head & neck squamous cell carcinoma: an overview.cancer statistics report an increased incidence of oscc and opscc around the world. though improvements in screening and early diagnosis have dramatically reduced the incidence of this neoplasm in recent years, the 5-year-disease-free survival, is still poor, specially for oropharyngeal cancer, despite the great scientific and financial efforts. recently, several papers showed that hpv may be involved at least in the pathogenesis of a subgroup of oral and cervical scc, leading to distinct molecu ...201121447181
increases in human papillomavirus detection during early hiv infection among women in zimbabwe.individuals who acquire human immunodeficiency virus (hiv) may experience an immediate disruption of genital tract immunity, altering the ability to mount a local and effective immune response. this study examined the impact of early hiv infection on new detection of human papillomavirus (hpv).201121451006
cost-effectiveness of different human papillomavirus vaccines in singapore.human papillomavirus (hpv) vaccines are widely available and there have been studies exploring their potential clinical impact and cost-effectiveness. however, few studies have compared the cost-effectiveness among the 2 main vaccines available - a bivalent vaccine against hpv 16/18, and a quadrivalent vaccine against 6/11/16/18. we explore the cost-effectiveness of these two hpv vaccines in tropical singapore.201121453537
human papillomavirus 16 e2 stability and transcriptional activation is enhanced by e1 via a direct protein-protein interaction.human papillomavirus 16 e1 and e2 interact with cellular factors to replicate the viral genome. e2 forms homodimers and binds to 12 bp palindromic sequences adjacent to the viral origin and recruits e1 to the origin. e1 forms a di-hexameric helicase complex that replicates the viral genome. this manuscript demonstrates that e1 stabilises the e2 protein, increasing the half life in both c33a and 293 t cells respectively. this stabilisation requires a direct protein--protein interaction. in additi ...201121458836
virucidal activity of the dendrimer microbicide spl7013 against hiv-1.topical microbicides for use by women to prevent the transmission of human immunodeficiency virus (hiv) and other sexually transmitted infections are urgently required. dendrimers are highly branched nanoparticles being developed as microbicides. spl7013 is a dendrimer with broad-spectrum activity against hiv type i (hiv-1) and -2 (hiv-2), herpes simplex viruses type-1 (hsv-1) and -2 (hsv-2) and human papillomavirus. spl7013 [3% (w/w)] has been formulated in a mucoadhesive carbopol gel (vivagel® ...201121459115
update on available vaccines in india: report of the appa vu 2010: i.the asia pacific pediatric association vaccinology update 2010 was held in mumbai on november 13-14, 2010 to discuss the latest information on burden of infectious diseases, recent developments in vaccines and their impact on immunization practices against infectious diseases occurring in indian children. during the conference the importance of including conjugate haemophilus influenzae type b vaccine and anti-rabies vaccines in routine immunization was stressed. also, the need for giving a seco ...201121373831
prevalence of anal intraepithelial neoplasia defined by anal cytology screening and high-resolution anoscopy in a primary care population of hiv-infected men and women.prevalence of high-grade anal intraepithelial neoplasia, the human papillomavirus-associated putative anal cancer precursor, is high in hiv-infected men who have sex with men, but less is known about its prevalence in other hiv-infected subgroups. similarly, the prevalence of abnormal cytology, used as a screen, is not well-defined in these subgroups.201121383563
lymphoepithelioma-like carcinoma of the urinary bladder: clinicopathologic, immunohistochemical, and molecular features.lymphoepithelioma-like carcinoma (lelc) in the urinary tract is a rare malignancy, named for its resemblance to nasopharyngeal undifferentiated carcinoma or lymphoepithelioma. investigation of immunohistochemical and molecular characteristics of bladder lelc is limited. the pathogenesis and biological behavior of these tumors are controversial.201121383609
cancer vaccine boosted by infrastructure for hiv care in africa. 201121383729
looking ahead: a case for human papillomavirus testing of self-sampled vaginal specimens as a cervical cancer screening strategy.even in the era of highly effective human papillomavirus (hpv) prophylactic vaccines, substantial reduction in worldwide cervical cancer mortality will only be realized if effective early detection and treatment of the millions of women already infected and the millions who may not receive vaccination in the next decade can be broadly implemented through sustainable cervical cancer screening programs. effective programs must meet three targets: (i) at least 70% of the targeted population should ...201121384341
mitochondrial c150t polymorphism increases the risk of cervical cancer and hpv infection.during a survey of control region (d-loop) sequence variances in 142 cervical cancer (cc) patients and 136 controls, all chinese women, including both hpv-positive (human papillomavirus) and hpv-negative subjects, we determined that the c150t polymorphism increased the cc risk in a case-control study (or=3.0, 95% ci=1.8-5.0, p<0.05). hpv-positive individuals were more likely to carry the c150t polymorphism than hpv-negative controls (or=5.8, 95% ci=2.6-13.2, p=2.3+ù10(-5)). hpv-positive cc patie ...201121385627
the burden of cervical cancer in china: synthesis of the evidence.the burden of cervical cancer in china has not been characterized in detail. we reviewed cervical cancer data from national mortality surveys and registries, and conducted a meta-analysis to estimate the prevalence of high-grade lesions (hsil) and high-risk human papillomavirus (hr-hpv) infections in rural shanxi province. we found that a national survey in the 1970s estimated age-standardized cervical cancer mortality rates as ôê+15 and ôê+83/100,000 women nationally and in xiangyuan, shanxi; b ...201121387308
combined promoter methylation analysis of cadm1 and mal: an objective triage tool for high-risk human papillomavirus dna-positive women.screening women for high-grade cervical intraepithelial neoplasia or cervical cancer (cin3(+)) by high-risk human papillomavirus (hrhpv) testing has as side-effect the detection of hrhpv-positive women without clinically relevant lesions. here, we developed an objective assay assessing the methylation status of the promoter regions of cadm1 and mal to triage hrhpv-positive women for cin3(+).201121389098
factors associated with intention to vaccinate a daughter against hpv: a statewide survey in alabama.human papillomavirus (hpv) is the most common sexually transmitted infection in the united states. adolescent and young adults aged 15-24 were estimated to account for three quarters of new infections in 2000. two hpv vaccines are currently available. the vaccine is recommended for girls aged 11-12 years. previous research has indicated that african-american and asian-american parents are less likely than caucasians to vaccinate their daughters. this investigation examines the relationship betwe ...201121397534
human papillomavirus 16 or 18 infection and prostate cancer risk: a meta-analysis.whether the oncogenic human papillomavirus (hpv) infection, especially infection with the most common subtypes 16 or 18, is related to prostate carcinogenesis remains conflicting. a meta-analysis with updated data was performed to obtain a more precise estimate of the association between them.201121400096
human papillomavirus vaccine: widening the scope for cancer prevention.human papillomavirus (hpv) is the necessary cause of cervical cancer. the hpv oncoproteins e6 and e7 have crucial roles in various steps of carcinogenesis, inducing degradation of p53 and destabilization of prb. several clinical trials show that recombinant hpv vaccines are safe and effective in preventing persistent infection of hpv and associated anogenital lesions. although most clinical studies to date have investigated the effectiveness of hpv vaccines in young female subjects, elderly fema ...201121465574
targeted cellular uptake and sirna silencing by quantum-dot nanoparticles coated with ß-cyclodextrin coupled to amino acids.quantum dots (qds) have the potential to serve as photostable beacons to track sirna delivery, which is fast becoming an attractive approach to probe gene function in cells. in this paper, we synthesized qd nanoparticles coated with ß-cyclodextrin (ß-cd) coupled to amino acids with different surface charges (positive, negative, and neutral) through direct ligand-exchange reactions and used them to deliver sirna. we found that these qds are diffluent in biological buffer with high colloidal stabi ...201121465588
large loop excision of transformation zone procedures used in the management of cytological abnormalities of the cervix.national health and medical research council (nhmrc) guidelines published in 2005 changed the management of cervical squamous intraepithelial lesions. asymptomatic low-grade abnormalities may be treated conservatively as many regress spontaneously. cervical changes reflect infection with the human papilloma virus (hpv). risk factors for acquiring hpv are the same as those for other sexually transmitted infections (stis).201121466510
prediction of residual/recurrent disease by hpv genotype after loop excision procedure for high-grade cervical intraepithelial neoplasia with negative margins.this study aims to evaluate whether the preconisation of high-risk human papillomavirus (hr-hpv) genotype and multiple hpv infection is predictive for residual/recurrent disease during the follow-up of high-grade cervical intraepithelial neoplasia (cin) treated by loop electrosurgical excision procedure (leep) with negative margins.201121466511
il-10 expression is regulated by hpv e2 protein in cervical cancer cells.it has been found that certain cytokines (il-4, il-10 and tgf-β1) are highly expressed locally in biopsies from patients with premalignant lesions and cervical cancer, and may induce a local immune-suppression state. in particular, il-10 is highly expressed in tumor cells and its expression is directly proportional to the development of hpv-positive cervical cancer, suggesting an important role of hpv proteins in the expression of il-10. in fact, we demonstrated that e6 and e7 hpv proteins regul ...201121468579
assessment of clinical and analytical performance characteristics of an hpv genotyping test.human papillomavirus (hpv), the known cause of cervical cancer, is found in essentially all cervical cancer specimens. infection with high-risk hpv genotypes carries the greatest risk of viral persistence and the potential to develop precancerous lesions or cervical cancer. identifying women infected with hpv 16 and/or 18, the two genotypes most commonly found in cervical cancer, helps further stratify women for either immediate referral to colposcopy or repeat cytological and hpv dna testing in ...201121472869
human papilloma virus associated head and neck cancer: a pcr based study.head and neck cancers (hnc), 90% of which are squamous cell carcinomas (scc), rank sixth among all malignancies worldwide and comprise 40-50% of the total number of malignancies in india. in addition to alcohol and tobacco usage, which is the major source of oral carcinogens, viruses such as human papilloma virus (hpv) may also contribute to development of the malignancy. the aim of this study was to identify the prevalence of hpv in head and neck cancers using material from metastatic site. a t ...201121472871
incidence of cervical intraepithelial neoplasia grade 2 or worse in colposcopy-negative/human papillomavirus-positive women with low-grade cytological abnormalities.please cite this paper as: kelly r, walker p, kitchener h, moss s. incidence of cervical intraepithelial neoplasia grade 2 or worse in colposcopy-negative/human papillomavirus-positive women with low-grade cytological abnormalities. bjog 2011; doi: 10.1111/j.1471-0528.2011.02970.x objective  to determine the risk of incident high-grade cervical intraepithelial neoplasia (cin) in human papillomavirus (hpv) -positive women with low-grade cytological abnormalities who had a satisfactory normal colp ...201121624034
prevalence and distribution of high risk human papillomavirus (hpv) types 16 and 18 in carcinoma of cervix, saliva of patients with oral squamous cell carcinoma and in the general population in karnataka, india.background: in view of conducting hpv vaccination in india it is most important to understand the prevalence of hpv genotypes in this population, not only in squamous cell carcinoma of cervix and oral cavity but also in the general population. in this study we explored the prevalence and distribution of high-risk hpv types 16 and 18 in carcinoma of cervix, saliva of patients with oral squamous cell carcinoma and in general population in karnataka. methods: cervical cancer specimens after punch b ...201121627358
detection of high-risk human papillomavirus types 16 and 18 but not 33 and 52 in external genital warts from iranian females.background: external genital warts (egw) are relatively common sexually transmitted diseases. in the majority of cases, low-risk human papilomaviruses (hpv), such as hpv-6 and hpv-11, are responsible but, high-risk types may also be detected and this has a bearing on vaccines for cervical cancer prevention. in this study the incidence of the high-risk hpv types 16, 18, 33 and 52 in egws of females from the southwest of iran was assessed. methods: seventy-nine women with egws participated in this ...201121627381
a competitive serological assay shows naturally acquired immunity to human papillomavirus infections in the guanacaste natural history study.background. a competitive luminex immunoassay (clia) has been developed to measure neutralizing antibodies against human papillomavirus (hpv) types 6, 11, 16 and 18. methods. in a cohort of 974 women from the guanacaste natural history study, we studied the relationship of baseline clia and virus-like particle (vlp) enzyme-linked immunosorbent assay (elisa) (hpv16 and hpv18 only) seropositivity to measures of hpv exposure, hpv dna positivity, number of sexual partners, cytology findings, and age ...201121628663
improved sensitivity of vaginal self-collection and high-risk human papillomavirus testing.background: self-collected vaginal specimens tested for high-risk human papillomavirus (hr-hpv) have been shown to be less sensitive for detection of cervical intraepithelial neoplasia or cancer (≥cin 3) than physician-collected endocervical specimens. to increase the sensitivity of self-collected specimens, we studied a self-sampling device designed to obtain a larger specimen from the upper vagina (poi/nih self-sampler) and a more sensitive pcr based hr-hpv assay. materials and methods: 10,000 ...201121630255
hpv vaccine against hpv infection and disease in males. 201121631334
an evaluation of a new dna test for carcinogenic human papillomavirus.using archived specimens, we evaluated a new automated real-time pcr assay (bd diagnostics), which detects all carcinogenic hpv and provides hpv genotyping for seven of them, including hpv16 and hpv18, the two most carcinogenic hpv genotypes. we found comparable results with hybrid capture 2 (hc2) for detection of carcinogenic hpv (n = 473) and with linear array and line blot assay (n = 371) for detection of individual hpv genotypes.201121632892
oncogenic potential of human papillomavirus (hpv) and its relation with cervical cancer.abstract: human papillomavirus (hpv) is the most common cause of cervical cancer. cervical cancer being the second most common cancer after lung cancer, affecting women of different age groups; has a prevalence of about 20% in young sexually active women. among different types of hpv, hpv16 the major strain causing this cancer and is sexually transmitted had been unnoticed for decades. keeping in mind the multiple risk factors related with cervical cancer such as early age sexual activities, tee ...201121635792
reduced mir-100 expression in cervical cancer and precursors and its carcinogenic effect through targeting plk1 protein.aim: although aberrant mirnas expression has been documented, altered mir-100 expression in cervical cancer and precursor tissues and its carcinogenic effect and mechanism remain unexplored. the aim of our study was to investigate the role of mir-100 alteration in cervical carcinogenesis. methods: the expression of mir-100 was examined by quantitative real-time reverse transcriptase pcr (qrt-pcr) in 125 cervical tissues including normal cervical epithelium, cervical intraepithelial neoplasia (ci ...201121636267
the epidemic of hpv-associated oropharyngeal cancer is here: is it time to change our treatment paradigms?although relatively uncommon, oropharyngeal cancers are increasing in incidence despite declining prevalence of smoking and in direct opposition to a decreasing incidence of all other head and neck cancers. an epidemic of human papillomavirus (hpv)-associated oropharyngeal cancers seems to account for these incidence trends. important demographic, behavioral, and prognostic characteristics define this unique population. changes in prevention, diagnosis, evaluation, staging, and treatment are nee ...201121636538
hpv protection in older groups may be in the eye of the beholder. 201121647126
quadrivalent papillomavirus vaccine in women over age 26. cervical screening must remain a priority. 201121648175
antitumor therapeutic and antimetastatic activity of electroporation-delivered human papillomavirus 16 e7 dna vaccines: a possible mechanism for enhanced tumor control.dna vaccines are known to be lacking in immunogenicity in humans. presently, electroporation (ep) is thought to overcome this limitation. here, we investigate whether human papillomavirus 16 e7 dna vaccines delivered by ep might elicit potent antitumor activity in animal cervical cancer models, with a focus on the underlying mechanism(s). intramuscular (im)-ep delivery of e7 dna vaccines induced more potent antitumor therapeutic and antimetastatic activity compared with im delivery. moreover, th ...201121649506
providing vaccines against human papillomavirus to adolescent girls in the americas: battling cervical cancer, improving overall health.vaccines against the human papillomavirus (hpv)--the primary cause of cervical cancer--target adolescent girls, many of whom have limited contact with health services. countries in the americas are beginning to use hpv vaccines to increase the impact of cervical cancer programs and as an entry point to broader health services for girls. this strategy opens new opportunities to improve lifelong health habits; encourage regular cervical cancer screening and treatment, when necessary; and offer ass ...201121653962
serum antibodies to the hpv16 proteome as biomarkers for head and neck cancer.human papillomavirus (hpv) type 16 is associated with oropharyngeal carcinomas (opc). antibodies (abs) to hpv16 e6 and e7 oncoproteins have been detected in patient sera; however, abs to other early hpv-derived proteins have not been well explored.201121654689
pcr study of a series of ascus cases hpv-positive by hcii.most guidelines currently recommend the testing of human papillomavirus (hpv) in ascus cases. the most used method for this purpose is hybrid capture ii (hcii), but pcr techniques with gp5+/6+ primers can be also applied. furthermore, the hcii high-risk probe test for detection of hpv shows cross-reactivity with low-risk hpv. although this cross-reactivity has been studied in screening populations, it has received little attention in ascus cases. to compare the performance of the hcii high-risk ...201121656701
prognostic implication of high risk human papillomavirus e6 and e7 mrna in patients with intraepithelial lesions of the cervix in relationship to age.since the introduction of the cytological screening programs, a significant reduction in the incidence of cervical cancer has been achieved. almost all of these cancers are related to high-risk (hr) human papillomavirus (hpv) cervical infections. however, the natural history of hpv infection seems to be different in younger patients, resulting in a higher rate of regression. there is, therefore, the need to identify hpv-related biomarkers in order to enhance the effectiveness of screening of hig ...201121658320
adult immunizations: update on recommendations.the advisory committee for immunization practices recommends universal influenza vaccination for 2010-2011. older adults should be offered protection against herpes zoster, and younger adults should receive immunization against human papilloma virus and pertussis. hepatitis b vaccination should be encouraged in non-immune adults. recommendations also address vaccinations for tetanus/diphtheria, hepatitis a, pneumococcus, measles/mumps/rubella, and meningococcus.201121658665
[investigation on hpv dna detection and genotyping practices used in french laboratories in 2009].the french national reference laboratory for human papillomavirus (hpv) performed in 2009 a national study in order to review the methods used to detect and identify hr hpv genotypes in microbiology laboratories. results from this study show a great diversity in volumes of samples treated in laboratories. among clinical indications, the most frequent is a result of asc-us at a pap smear. this indication in the only one covered by the national public insurance system and is mostly performed in la ...201121659046
meeting of the who human papillomavirus vaccine advisory committee, april 2010. 201121661272
new technologies and advances in colposcopic assessment.to have a good grasp of clinical colposcopy, it is necessary to understand the histopathologic structure of the normal and dysplastic cervical epithelium. previous meta-analyses had indicated high overall sensitivity of colposcopy in detecting dysplastic lesions, but recent studies have suggested that the technique has much lower sensitivity in detecting high-grade intraepithelial neoplasia. the best practice in colposcopy relies on accurately taking a biopsy from the correct (i.e. most morpholo ...201121664876
human papillomavirus dna and mrna positivity of the anal canal in women with lower genital tract hpv lesions: predictors and clinical implications.women with hpv related pathology of the lower genital tract are at higher risk for ain and anal cancer than the general population. a strategy to identify anal disease in these women has not been formulated. the aim of this study is to examine the feasibility of hpv related biomarker testing on anal smears, to identify the risk factors for anal hpv positivity and to provide information of the clinical implications of anal hpv infection in this population.201121665253
the role of human papillomavirus on sperm function.to review the role of human papillomavirus (hpv) on sperm parameters, fertility and implication of the use of infected sperm cells in assisted reproduction.201121666465
transforming activity of a novel mutant of hpv16 e6e7 fusion gene.an optimized recombinant hpv16 e6e7 fusion gene (hpv16 ofe6e7) was constructed according to codon usage for mammalian cell expression, and a mutant of hpv16 ofe6e7 fusion gene (hpv16 omfe6e7) was generated by site-directed mutagenesis at l57g, c113r for the e6 protein and c24g, e26g for the e7 protein for hpv16 ofe6e7 [patent pending (cn 101100672)]. the hpv16 omfe6e7 gene constructed in this work not only lost the transformation capability to nih 3t3 cells and tumorigenicity in scid mice, but a ...201121667341
higher micronutrient intake is associated with human papillomavirus-positive head and neck cancer: a case-only analysis.no studies have investigated dietary differences between head and neck squamous cell carcinoma (hnscc) patients with human papillomavirus (hpv)-positive tumors and patients with hpv-negative tumors. this study was designed to investigate the relationship between diet and hpv status in hnscc patients. cases of hnscc were recruited from 2 clinical centers participating in the university of michigan head and neck specialized program of research excellence (spore). hpv tissue genotyping was performe ...201121667401
racial disparities in awareness of the human papillomavirus.abstract background: human papillomavirus (hpv) is the most common sexually transmitted infection in the united states yet is one of the least recognized among the general public. hpv awareness may be relatively low among minority women. because hpv awareness is associated with uptake of the hpv vaccine, it is critical to assess hpv awareness in the population and identify any racial/ethnic gaps. methods: this study used nationally representative data collected by the national survey of family g ...201121668381
human papillomavirus genotype distribution among french women with and without cervical abnormalities.to assess human papillomavirus (hpv) prevalence and distribution among french women with normal and pathologic cytology findings.201121669421
serum antibody response following genital {alpha}9 human papillomavirus infection in young men.background.ôçâalthough the prevalence of human papillomavirus (hpv) genital infection is similarly high in males and females, seroprevalence is lower in males. this study assessed rates and determinants of seroconversion after detection of genital hpv infection in young men. methods.ôçâwe investigated hpv type-specific seroconversion in a cohort of heterosexual male university students who had an ╬▒9 hpv type (hpv-16, -31, -33, -35, -52, -58, or -67) detected in the genital tract (n = 156). hpv ...201121673030
postlicensure safety evaluation of human papilloma virus vaccines.a summary of postlicensure safety information of hpv vaccines from three sources: vigibase, the global database of who's programme for international drug monitoring, the vaers report on gardasil and the rivm report on cervarix.201121673418
evolution and taxonomic classification of human papillomavirus 16 (hpv16)-related variant genomes: hpv31, hpv33, hpv35, hpv52, hpv58 and hpv67.human papillomavirus 16 (hpv16) species group (alpha-9) of the alphapapillomavirus genus contains hpv16, hpv31, hpv33, hpv35, hpv52, hpv58 and hpv67. these hpvs account for 75% of invasive cervical cancers worldwide. viral variants of these hpvs differ in evolutionary history and pathogenicity. moreover, a comprehensive nomenclature system for hpv variants is lacking, limiting comparisons between studies.201121673791
inverted papilloma of the cervix and vagina: report of 2 cases of a rare lesion associated with human papillomavirus 42.we report 2 cases of a lesion that we term inverted papilloma of the lower female genital tract, occurring in the cervix and upper vagina of 60- and 50-year-old women, respectively. microscopically, the features were similar to those of inverted transitional papilloma of the urinary bladder with interconnecting islands, trabeculae, and solid sheets of bland transitional epithelium with an inverted growth pattern. there were small foci of squamous and glandular differentiation in the cervical cas ...201121813160
human papillomavirus typing of warts and response to cryotherapy.background  cutaneous warts are common and caused by a number of different types of human papillomaviruses (hpvs). objective  the aim of this study was to investigate the hpv types causing common warts and to determine any association between the hpv type and the duration of warts and response to cryotherapy. methods  eighty wart samples from 76 immunocompetent patients were taken from warts by paring prior to cryotherapy and analysed by in situ hybridization (ish) with hpv probes specific to hp ...201121812829
characterization of a novel cutaneous human papillomavirus genotype hpv-125.the dna genome of a novel hpv genotype, hpv-125, isolated from a hand wart of an immuno-competent 19-year old male was fully cloned, sequenced and characterized. the full genome of hpv-125 is 7,809-bp in length with a gc content of 46.4%. by comparing the nucleotide sequence of the complete l1 gene, hpv-125 is phylogenetically placed within cutaneotrophic species 2 of alphapapillomaviruses, and is most closely related to hpv-3 and hpv-28. hpv-125 has a typical genomic organization of alphapapill ...201121811601
risk factors for persistent cervical intraepithelial neoplasia grades 1 and 2: managed by watchful waiting.objective:: this study examines risk factors for persistent cervical intraepithelial neoplasia (cin) and examines whether human papillomavirus (hpv) testing predicts persistent lesions. materials and methods:: women with histologically diagnosed cin 1 or cin 2 (n = 206) were followed up every 3 months without treatment. human papillomavirus genotyping, plasma levels of ascorbic acid, and red blood cell folate levels were obtained. cervical biopsy at 12 months determined the presence of cin. rela ...201121811178
development of a 2-plex luminex-based competitive immunoassay to quantify neutralizing antibodies induced by virus-like particles for human papillomavirus 16 and 18.human papillomavirus (hpv) l1 virus-like particles (vlps) were proven an effective vaccine candidate to prevent against hpv-16 and -18 infections. in order to evaluate the potency of our produced hpv-16 and -18 l1 vlps-based vaccine candidates, also to quantify neutralizing antibodies induced by them, a 2-plex luminex-based competitive immunoassay was developed. unlike the published paper, the no-biotin conjugated neutralizing mabs spiked normal human serum (nhs) was used for standard curve prep ...201121808597
development of interdigitated arrays coated with functional polyaniline/mwcnt for electrochemical biodetection: application for human papilloma virus.in this study, polyaniline-multiwalled carbon nanotube film (pani-mwcnt) has been polymerized on interdigitated platinum electrode arrays (ida), fabricated by mems technology for the detection of human papillomavirus (hpv) infection, using immobilized peptide aptamers as affinity capture reagent. label-free, electrochemical detection of the specific immune reaction between antigen peptide aptamer hpv-16-l1 (with a molecular weight of 1825da), the most common genotype in cytological normal women ...201121807222
evaluation of a novel microplate colorimetric hybridization genotyping assay for human papillomavirus.persistent infection with high-risk human papillomavirus (hr-hpv) has been associated with cervical cancer. developing assays for the identification of these viral types is of great importance for monitoring patients and controlling strategies. the development of the mcha (microplate colorimetric hybridization assay), a pcr-based method for identifying six of the most common hr-hpv types (hpv 16, 18, 31, 33, 39 and 45) is described. the mcha combines the amplification with the gp5+/gp6+ consensu ...201121807028
expression of p16 in sinonasal undifferentiated carcinoma (snuc) without associated human papillomavirus (hpv).sinonasal undifferentiated carcinoma (snuc) is an uncommon and highly aggressive neoplasm of the paranasal sinuses and nasal cavity. its undifferentiated histologic appearance often requires immunohistochemical studies to distinguish it from other high-grade neoplasms. due to the rarity of snuc, its immunohistochemical staining profile has been incompletely characterized, and little work has been done on its expression of the markers for human papillomavirus (hpv). our objective is to expand our ...201121805120
chemotherapeutic alteration of vegf-/pdgf- and pdgf-ra/ß expression by imatinib in hpv-transformed squamous cell carcinoma compared to hpv-negative hnscc in vitro.head and neck squamous cell carcinoma (hnscc) is an aggressive epithelial malignancy known to be the most common neoplasm appearing in the upper aerodigestive tract. the poor five-year survival rate has remained unchanged in the last decades despite the emergence of improved techniques in surgery, radiation and chemotherapy. in the last 20 years awareness of a subset of squamous cell carcinomas induced by oncogenic forms of the human papilloma virus (hpv) (high-risk types 16 and 18) has increase ...201121805039
evaluating a county-sponsored social marketing campaign to increase mothers' initiation of hpv vaccine for their pre-teen daughters in a primarily rural area.routine vaccination against human papillomavirus (hpv), the main cause of cervical cancer, is recommended for 11-12 year old girls, yet vaccine uptake is low. this study evaluates a social marketing campaign initiated by 13 north carolina counties to raise awareness among parents and reduce barriers to accessing the vaccine in a primarily rural area. the 3-month campaign targeted mothers of girls ages 11-12 and healthcare practices serving pre-teen girls in four counties. principles of social ma ...201121804767
high-risk human papillomavirus is associated with hiv acquisition among south african female sex workers.background. mounting evidence suggests an association between human papillomavirus (hpv) and hiv acquisition. this study aimed to explore this association among south african female sex workers (fsws). methods. we used data from 88 hiv-negative fsws who participated in a vaginal gel (col-1492) trial. cervicovaginal rinse samples, obtained before hiv-seroconversion, were genotyped into high-risk (hr-) and low-risk (lr-) hpv. hiv-adjusted hazard ratios (ahrs) and 95% confidence intervals (ci) were ...201121804752
role of via in cervical cancer screening in low-resource countries.cervical cancer is one of the few highly preventable cancers. the early detection and removal of precancerous cervical lesions effectively abolish the development of invasive cervical cancer. the pap test has been the standard screening test in the western world for the last five decades. visual inspection of cervix with acetic acid (via) is currently more popular method of cervical cancer of screening test in low resource countries. cervical cancer incidence and mortality have been reduced dram ...201121804524
human papillomavirus genotyping by 454 next generation sequencing technology.background: an accurate tool for human papillomavirus (hpv) typing is important both for management of patients with hpv infection and for surveillance studies. objectives: design and evaluation of an hpv typing method based on 454 next generation sequencing (ngs) technology. study design: development of an hpv typing method based on 454 ngs of hpv l1 amplicons generated with my09/11-based primers. evaluation of the ngs method in control samples and in a panel of cervical cytological samples. co ...201121802982
pre-vaccination genomic diversity of human papillomavirus genotype 6 (hpv 6): a comparative analysis of 21 full-length genome sequences.comparative analysis of 21 full-length genome sequences of human papillomavirus genotype 6 (hpv 6): 18 determined in this study and three sequences available in nucleotide sequence databases, revealed more than 98% nucleotide similarity to the hpv 6 prototype isolate. the minimum and maximum genomic distance between the full-length genomic variants and the prototype sequence was three nucleotide substitutions, and 122 nucleotide substitutions and three insertions, respectively. detailed sequence ...201121801853
safety and immunogenicity of human papillomavirus-16/18 as04-adjuvanted vaccine in healthy chinese females aged 15 to 45 years: a phase i trial.globally, about 70% of cervical cancers are associated with human papillomavirus (hpv)-16 or hpv-18 infection. a meta-analysis of epidemiologic studies in china showed that hpv was present in 98% of cervical cancer samples. the hpv-16/18 as04-adjuvanted vaccine cervarix has shown a high level of protection against hpv-16/18 infections and associated cervical lesions. this phase i trial (nct00549900) assessed the safety, tolerability, and immunogenicity of the vaccine in chinese. thirty healthy c ...201121801604
a population-based, cross-sectional study of age-specific risk factors for high risk human papillomavirus prevalence in rural nigeria.abstract: background: cervical cancer, caused by persistent infection with carcinogenic human papillomavirus (hr-hpv), is particularly prevalent in sub-saharan africa and is associated with a high mortality rate. some studies in west africa, including our own, have found unusually high hr-hpv across all ages with a slight peak in older women. this increased prevalence may complicate screen-and-treat programs, which are implemented in regions where hpv prevalence declines with age and typically t ...201121801395
exome sequencing of head and neck squamous cell carcinoma reveals inactivating mutations in notch1.head and neck squamous cell carcinoma (hnscc) is the sixth most common cancer worldwide. to explore the genetic origins of this cancer, we used whole-exome sequencing and gene copy number analyses to study 32 primary tumors. tumors from patients with a history of tobacco use had more mutations than did tumors from patients who did not use tobacco, and tumors that were negative for human papilloma virus (hpv) had more mutations than did hpv-positive tumors. six of the genes that were mutated in m ...201121798897
lymphoepithelial-like carcinoma of the uterine cervix; a case report.lymphoepithelioma-like carcinoma (lelc) of the uterine cervix is a rare variant of squamous cell carcinoma of the uterine cervix. this tumor is characterized by nests of poorly differentiated epithelial cells surrounded by a prominent lymphocytic infiltration. despite the poorly differentiated pathological findings, it appears to have a better outcome than the usual squamous cell carcinoma of the uterine cervix. therefore, it is quite important to differentiate this tumor from poorly differentia ...201121797126
immunological evaluation of vaginal secretion in patients with high-grade cervical intraepithelial neoplasia treated with intralesional interferon alpha-2b.conservative treatment with intralesional interferon (ifn) is a therapeutic option for cervical intraepithelial neoplasia (cin) patients of childbearing age.201121797121
xrcc1 arg399gln polymorphism and risk for cervical cancer development in argentine women.xrcc1 (x-ray repair cross-complementing group 1) plays a central role in the dna base excision repair mechanism. single nucleotide polymorphisms (snps) in the xrcc1 gene are thought to modulate dna repair capacity and have been linked to cancer risk in several studies.201121797115
pre-vaccination epidemiology of human papillomavirus infections in japanese women with abnormal cytology.aim: to investigate the pre-vaccination epidemiology of genital human papillomavirus (hpv) infections and genotypes in women with abnormal cytology in nagasaki, japan. material and methods: we performed pap smear tests, biopsies and hpv genotype testing in nagasaki prefecture from august 2007 through november 2009. results: during the study period, serial samples of uterine cervical specimens were obtained from 539 subjects with abnormal cytology and/or squamous intraepithelial lesions (sil) ...201121793993
usefulness of serum mass spectrometry to identify women diagnosed with higher grades of cervical intraepithelial neoplasia may differ by race.an early detection of precursor lesions of cervical cancer will help to eliminate the worldwide burden of cervical cancer.201121792340
screening for the prevention of cervical cancer in the era of human papillomavirus vaccination: an australian perspective.australia has a unique and highly successful screening program for cervical cancer which is based on the conventional pap smear. since its introduction in 1991 there has been a decline in both the incidence of and mortality from this disease. part of the success of this program has been the introduction of pap test registers and strict quality assurance measures for cervical cytology, including compulsory key performance indicators for laboratories. using these measures, nationwide calculations ...201121791898
prevalence of genital human papillomavirus among females in the united states, the national health and nutrition examination survey, 2003-2006.background. genital human papillomaviruses (hpv) include >40 sexually transmitted viruses. most hpv infections do not progress to disease, but infection with certain types of hpv can cause cervical and other anogenital and oropharyngeal cancer, and other types of hpv are associated with anogenital warts. hpv vaccines prevent infection with hpv 16 and 18, which account for 70% of cases of cervical cancer, and hpv 6 and 11, which cause 90% of the cases of anogenital warts. methods. using data an ...201121791659
human papillomavirus type 16 e6 inhibits p21(waf1) transcription independently of p53 by inactivating p150(sal2).hpv16 e6 deregulates g1/s cell cycle progression through p53 degradation preventing transcription of the cdk inhibitor p21(waf1). however, additional mechanisms independent of p53 inactivation appear to exist. here, we report that hpv16 e6 targets the cellular factor p150(sal2), which positively regulates p21(waf1) transcription. hpv16 e6 associates with p150(sal2), inducing its functional inhibition by preventing its binding to cis elements on the p21(waf1) promoter. a hpv16 e6 mutant, l110q, w ...201121791360
psychological responses of women infected with cervical human papillomavirus: a qualitative study in taiwan.to determine the psychological response of cervical human papillomavirus (hpv) infected women.201121791300
integration of human papillomavirus correlates with high levels of viral oncogene transcripts in cervical carcinogenesis.a prospective, cross-sectional study was conducted to investigate the correlation between the integration of high-risk human papillomavirus and disease severity of cervical lesions. 720 liquid-based cytology specimens including 422 normal cytology, 78 low-grade squamous intraepithelial lesions, 172 high-grade squamous intraepithelial lesions, and 48 women with cervical cancers were examined using hpv blot and type-specific e6 pcr. positive hpv dna types 16, 18, 52 and 58 were examined for viral ...201121777630
emerging theme: cellular pdz proteins as common targets of pathogenic viruses.more than a decade ago three viral oncoproteins - adenovirus type 9 e4-orf1, human t-lymphotropic virus type 1 tax, and high-risk human papillomavirus e6 - were found to encode a related carboxyl-terminal pdz domain-binding motif (pbm) that mediates interactions with a select group of cellular pdz proteins. recent studies have shown that many other viruses also encode pbm-containing proteins that bind to cellular pdz proteins. interestingly, these recently recognized viruses include not only som ...201121775458
histopathologic follow-up and human papillomavirus dna test results in 290 patients with high-grade squamous intraepithelial lesion papanicolaou test results.background: the study documents histopathologic outcomes and high-risk (hr) human papillomavirus (hpv) test results in a large cohort of patients with high-grade squamous intraepithelial lesion (hsil) liquid-based cytology (lbc) pap test results. methods: a total of 352 patients with hsil results (338 cervical and 14 vaginal) who had hrhpv testing and 290 patients with biopsy follow-up were studied. hrhpv detection rates were compared at different ages, with or without an endocervical/transforma ...201121774093
incomplete knowledge - unclarified roles in sex education: results of a national survey about human papillomavirus infections.marek e., dergez t., bozsa s., gocze k., rebek-nagy g., kricskovics a., kiss i., ember i. & gocze p. (2011) european journal of cancer care. incomplete knowledge - unclarified roles in sex education: results of a national survey about human papillomavirus infections the purpose of this study was to evaluate the awareness of human papillomavirus (hpv) infections both in male and female adults in hungary. a self-administered, anonymous questionnaire was completed by 785 college students and parent ...201121771125
small molecule inhibitors of human papillomavirus protein - protein interactions.human papillomaviruses (hpv) have now been identified as a necessary cause of benign and malignant lesions of the differentiating epithelium, particularly cervical cancer, the second most prevalent cancer in women worldwide. while two prophylactic hpv vaccines and screening programs are available, there is currently no antiviral drug for the treatment of hpv infections and associated diseases. the recent progress toward the identification and characterization of specific molecular targets for sm ...201121769307
the first international standard for antibodies to hpv 16.current hpv vaccines and vaccine candidates are based on recombinant virus capsid proteins, so called virus-like particles (vlps). standardisation of assays for hpv capsid antibody will assist with epidemiology studies and future vaccine development. a world health organization international collaborative study was undertaken to assess the suitability of a freeze-dried serum, obtained from women naturally infected with hpv 16 and reactive against hpv 16 only, to serve as the international standa ...201121767589
human papillomavirus genotype distribution in czech women and men with diseases etiologically linked to hpv.the hpv prevalence and genotype distribution are important for the estimation of the impact of hpv-based cervical cancer screening and hpv vaccination on the incidence of diseases etiologically linked to hpvs. the hpv genotype distribution varies across different geographical regions. therefore, we investigated the type-specific hpv prevalence in czech women and men with anogenital diseases.201121765924
new human papillomavirus (hpv) types involved in epidermodysplasia verruciformis (ev) in 3 hiv-infected patients: response to topical cidofovir. 201121763546
skin tumor formation in human papillomavirus 8 transgenic mice is associated with a deregulation of oncogenic mirnas and their tumor suppressive targets.background: dysregulation of microrna (mirna) expression is regularly found in various types of cancer and contributes to tumorigenic processes. however, little is known about mirna expression in non-melanoma skin cancer in which a pathogenic role of beta human papillomaviruses (hpv) is discussed. a carcinogenic potential of beta hpv8 could be demonstrated in a transgenic mouse model, expressing all early genes of hpv8 (hpv8-cer). a single uva/b-dose induced oncogene expression and led to papill ...201121763111
human papillomavirus type 18 chimeras containing the l2/l1 capsid genes from evolutionarily diverse papillomavirus types generate infectious virus.papillomaviruses (pvs) comprise a large family of viruses infecting nearly all vertebrate species, with more than 100 human pvs identified. our previous studies showed that a mutant chimera hpv18/16 genome, consisting of the upper regulatory region and early orfs of hpv18 and the late orfs of hpv16, was capable of producing infectious virus in organotypic raft cultures. we were interested in determining whether the ability of this chimeric genome to produce infectious virus was the result of hpv ...201121762735
higher incidence and persistence of high-risk human papillomavirus infection in female sex workers compared with women attending family planning.background: there are no data on the incidence and persistence of high-risk human papillomavirus (hr-hpv) infections in female sex workers (fsws). we aimed to describe and compare the rates of incidence and persistence of hr-hpv infections in fsws and women from the general population (wgp) who attended healthcare facilities between may 2003 and december 2006 in alicante, spain. methods: women with an established hr-hpv infection at study entry were evaluated for the analysis of hr-hpv persisten ...201121757383
human papillomavirus: science and technologies for the elimination of cervical cancer.introduction: academic research has made a significant advancement in understanding the viral causes of cervical cancer and generating the technology for prevention, both at the primary and secondary levels. human papillomaviruses (hpvs) have been recognized as the first necessary cause of cervical cancer, the second most common cancer in women worldwide. areas covered: this paper reviews the epidemiological evidence of the causality of hpv in relation to cervical cancer, other genital tract can ...201121756205
self-sample hpv-tests as an intervention for non-atteendees of cervical cancer screening in finland: a randomised study.background: attendance in screening is an important determinant of cervical cancer. previous experience on high-risk human papillomavirus (hrhpv) dna testing on patient-obtained samples suggests a good effect among non-attendees of screening. we assessed the effects of self-sampling on attendance in the finnish screening programme.methods: non-attendees after the primary invitation in one municipality (espoo) were randomised to receive either a self-sampling kit (2,397 women) or an extra invitat ...201121752985
incidence and clinicopathologic behavior of uterine cervical carcinoma in renal transplant recipients.abstract: background: renal allograft recipients are reported to have a higher incidence of malignancy than the general population. this single hospital-based study examined the incidence and clinicopathologic behavior of uterine cervical carcinoma in renal transplant recipients. methods: among 453 women receiving renal transplantation from january 1990 to december 2008, 5 patients were diagnosed with cervical carcinoma. medical records of these 5 patients were retrospectively reviewed, and clin ...201121752252
oral hpv infection and sexuality: a cross-sectional study in women.human papillomavirus (hpv) is the main risk factor for cervical cancers and is associated with close to 36% of oropharyngeal cancers. there is increasing evidence that oral hpv transmission is related to sexual behavior but to our knowledge studies that involve women who have sex with women have not been performed. we examined the prevalence of oral hpv according to sexual behavior among a population-based sample of 118 women and have made some inferences of possible predictors of oral hpv infec ...201121747715
an examination of male and female odds ratios by bmi, cigarette smoking, and alcohol consumption for cancers of the oral cavity, pharynx, and larynx in pooled data from 15 case-control studies.greater tobacco smoking and alcohol consumption and lower body mass index (bmi) increase odds ratios (or) for oral cavity, oropharyngeal, hypopharyngeal, and laryngeal cancers; however, there are no comprehensive sex-specific comparisons of ors for these factors.201121744095
human papillomavirus genotype testing combined with cytology as a 'test of cure' post treatment: the importance of a persistent viral infection.background: human papillomavirus (hpv) testing has been evaluated as a test of cure in patients following treatment of high-grade cervical intraepithelial neoplasia (cin2+). studies show that women who are hpv and cytology negative post treatment can be safely returned to routine recall. the management strategy for hpv positive women requires confirmation. objective: to evaluate the clinical utility of the papillocheck(-«) genotyping assay for predicting disease recurrence in a test of cure sett ...201121831706
human papillomavirus types 16 and 18 in epithelial dysplasia of oral cavity and oropharynx: a meta-analysis, 1985-2010.human papillomavirus (hpv) types 16 and 18 are causally related to a sub-set of oral cavity and oropharyngeal squamous cell cancers. however, a clear estimate of the prevalence of hpv-16/18 in oral cavity and oropharyngeal dysplasia (oopd) is not available. this literature review and meta-analysis was conducted to provide a prevalence estimate for hpv-16/18 in oopd. twenty-two studies that reported prevalence of hpv-16 and/or 18 in 458 oopd lesions were analyzed. meta-analysis was used to evalua ...201121816661
Refresh
firstprev
12345678910...
nextlast
Displaying items 301 - 400 of 6259