Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| human respiratory syncytial virus in children hospitalized for acute lower respiratory infection. | to evaluate the prevalence and seasonality of human respiratory syncytial virus (hrsv) in children aged 0 to 6 years, hospitalized with acute lower respiratory infection (alri) in são josé do rio preto, sp, brazil, and the association between age, diagnosis, and hrsv. | 2011 | 21461452 |
| including viral infection data supports an association between particulate pollution and respiratory admissions. | to refine and revise previous air pollution, climate and health time series analysis in christchurch, new zealand, introducing viral identification data (positive identification count and outbreak, defined as two of more positive tests). | 2011 | 21463414 |
| sheng-ma-ge-gen-tang (shoma-kakkon-to) inhibited cytopathic effect of human respiratory syncytial virus in cell lines of human respiratory tract. | sheng-ma-ge-gen-tang (smggt; shoma-kakkon-to) has been used against pediatric viral infection for thousands of year in ancient china. however, it is unknown whether smggt is effective against human respiratory syncytial virus (hrsv). | 2011 | 21463671 |
| acute lower respiratory tract infection due to respiratory syncytial virus in a group of egyptian children under 5 years of age. | respiratory syncytial virus (rsv) is one of the most important causes of acute lower respiratory tract infections (alrti) in infants and young children. this study was conducted to describe the epidemiology of alrti associated with rsv among children = 5 years old in egypt. | 2011 | 21466713 |
| detection of human metapneumovirus in infants with acute respiratory tract infection. | both respiratory syncytial virus (rsv) and human metapneumovirus (hmpv), a recently discovered respiratory pathogen classified in the paramyxoviridae family, have been associated with acute respiratory tract infections (arti) in infants. the aim of our study was to determine the incidence and clinical features of hmpv infection in infants presenting with arti at a tertiary children's hospital in greece during the 2007-2008 and 2008-2009 winter seasons. routine nasal swabs were obtained from 127 ... | 2011 | 21468562 |
| detection of microorganisms in exhaled breath condensate during acute exacerbations of copd. | summary at a glance: exhaled breath condensate (ebc) is a noninvasive method to assess airway biology. the aim of the present study was to evaluate ebc for the detection of microbial nucleic acids during acute exacerbations of copd (aecopd). bacterial nucleic acids can be identified in ebcs of copd patients with exacerbations, however, the results differed significantly from those of sputum. abstract: background and objective: one hallmark of chronic obstructive pulmonary disease (copd) is colo ... | 2011 | 21470340 |
| viral-mediated inhibition of antioxidant enzymes contributes to the pathogenesis of severe respiratory syncytial virus bronchiolitis. | rationale: respiratory syncytial virus (rsv) is a major cause of lower respiratory tract infections in children, for which no specific treatment or vaccine is currently available. we have previously shown that rsv induces reactive oxygen species in cultured cells and oxidative injury in the lungs of experimentally infected mice. the mechanism(s) of rsv-induced oxidative stress in vivo is not known. objectives: to measure changes of lung antioxidant enzymes expression/activity and activation of n ... | 2011 | 21471094 |
| differential pathogenesis of respiratory syncytial virus clinical isolates in balb/c mice. | airway mucus is a hallmark of respiratory syncytial virus (rsv) lower respiratory tract illness. laboratory rsv strains differentially induce airway mucus production in mice. here, we tested the hypothesis that rsv strains differ in pathogenesis by screening six low-passage rsv clinical isolates for mucogenicity and virulence in balb/cj mice. the rsv clinical isolates induced variable disease severity, lung interleukin-13 (il-13) levels, and gob-5 levels in balb/cj mice. we chose two of these cl ... | 2011 | 21471228 |
| respiratory syncytial virus infection is associated with an altered innate immunity and a heightened pro-inflammatory response in the lungs of preterm lambs. | abstract: | 2011 | 21827668 |
| viral co-detection in infants hospitalized with respiratory disease: is it important to detect? | 2011 | 21842109 | |
| replacement of previously circulating respiratory syncytial virus subtype b strains with the ba genotype in south africa. | respiratory syncytial virus (rsv) is a major cause of bronchiolitis and pneumonia in infants, the immunocompromised, and the elderly in both developed and developing countries. reinfections are common, and g protein variability is one mechanism to overcome herd immunity. this is illustrated by the appearance of the ba genotype with a 60-nucleotide duplication dominating the subtype b genotypes in epidemics worldwide. to investigate the evolution of subtype b in south africa since 2002, the genet ... | 2011 | 21715483 |
| breathing easy: implementing a bronchiolitis protocol. | in 2005, a protocol for patients with bronchiolitis in the pediatric unit was created, but compliance was less than desired. therefore, revisions based on staff feedback and current literature were implemented in 2007. a pre- and post-implementation design was used. descriptive statistics were used to measure the following variables: use of protocol, frequency of suctioning, use of beta2 agonist medication, length of stay, and charges per case. a chart review was used. after implementing the rev ... | 2011 | 21739745 |
| prevalence of respiratory pathogens in diseased, non-vaccinated, routinely medicated veal calves. | the prevalence of respiratory pathogens in diseased veal calves was determined in 24 respiratory disease outbreaks in 15 herds in belgium. bacteria were cultured from nasopharyngeal swabs and seroconversion against viruses and mycoplasma bovis was determined on paired sera. at the individual calf level, mycoplasma species, mannheimia haemolytica and pasteurella multocida, were isolated from 70.5 per cent, 21.5 per cent and 26.0 per cent of swabs, respectively. at the herd level, the presence of ... | 2011 | 21831999 |
| increased eosinophilic cationic protein in nasal fluid in hospitalized wheezy infants with rsv infection. | background: respiratory syncytial virus (rsv) is a major respiratory pathogen which causes bronchiolitis with dyspnea and wheezing in children less than 2 years old. rsv bronchiolitis in infancy severe enough to cause hospitalization might be a risk factor for allergic sensitization and bronchial asthma in future. however, the pathophysiology behind this development has not been clearly characterized. to evaluate the existence of airway inflammation and characteristic of rsv bronchiolitis, we an ... | 2011 | 21681019 |
| [contemporary clinical diagnostics of respiratory tract infections]. | the patient population at risk pulmonary infections has increased during the last decade. the spectrum of organisms causing infections has also grown, most of them are viruses baseline diagnostic assessment include history, clinical examination, radiography and measurements of procalcitonin (pct), highly sensitive c-reactive protein (hscrp) and leukocyte count. pct represents a good biological marker for difficult diagnoses in critically ill patients and is superior to crp. chest x-rays seldom g ... | 2011 | 21675131 |
| secondary lymphoid tissue as an important site for wu polyomavirus infection in immunocompetent children. | the polyomaviruses ki and wu (kipyv and wupyv) have been identified in respiratory specimens from children with acute respiratory infections, which suggests the respiratory tract as a possible site of infection. however, the persistence of infection in the lymphoid system is unknown. fresh samples (n = 211) of tonsils, adenoids, and peripheral blood mononuclear cells (pbmcs) from 83 immunocompetent children (mean age 4.8 years) were tested for amplification of the kipyv vp1 and wupyv vp2 genes. ... | 2011 | 21678449 |
| attenuated interleukin-8/leukocyte immunoresponse in preterm infants compared with term infants hospitalized with respiratory syncytial virus bronchiolitis: a pilot study. | decreased transplacental transfer of antibodies and altered immunoresponsiveness may place preterm (pt) infants at higher risk for serious consequences from respiratory syncytial virus (rsv) bronchiolitis. we hypothesize that among infants hospitalized with rsv bronchiolitis, immune response in pt infants may be different when compared with that of term infants. nasal-wash samples were collected from 11 pt (<37 weeks of gestation) and 13 term infants (ôëñ37 weeks of gestation) hospitalized with ... | 2011 | 21683109 |
| antibiotics for bronchiolitis in children. | bronchiolitis is a serious, potentially life-threatening respiratory illness commonly affecting babies. it is often caused by respiratory syncytial virus (rsv). antibiotics are not recommended for bronchiolitis unless there is concern about complications such as secondary bacterial pneumonia or respiratory failure. nevertheless, they are used at rates of 34% to 99% in uncomplicated cases. | 2011 | 21678346 |
| respiratory syncytial virus infection in children with congenital heart disease: global data and interim results of korean rsv-chd survey. | respiratory syncytial virus (rsv) is a main cause of hospitalization for bronchiolitis and pneumonia in infants worldwide. children with hemodynamically significant congenital heart disease (hs-chd), as well as premature infants are at high risk for severe rsv diseases. mortality rates for chd patients hospitalized with rsv have been reported as about 24 times higher compared with those without rsv infection. recently with advances in intensive care, mortality rates in chd patients combined with ... | 2011 | 21829409 |
| confirmation of an association between single nucleotide polymorphisms in the vdr gene with respiratory syncytial virus related disease in south african children. | respiratory syncytial virus is a leading cause of lower respiratory tract infection in infants. disease severity has been linked to host immune responses and polymorphisms in genes associated with innate immunity. a large-scale genetics study of single nucleotide polymorphisms (snps) in children in the netherlands identified snps in the vitamin d receptor (vdr) and jun genes which have a strong association with an increased risk of developing bronchiolitis following the first respiratory syncyti ... | 2011 | 21837802 |
| [acute viral respiratory infection in children under 5 years: epidemiological study in two centers in buenos aires, argentina]. | introduction. acute respiratory infections (ari) are a major cause of hospitalization particularly at the extreme ages of life. objectives. to determine the viral diagnosis in children < 5 years old with ari, seasonality, clinical and epidemiological characteristics. population and methods. a cross-sectional, descriptive and multicenter study was performed. respiratory samples from patients with ari hospitalized or outpatients with < 5 days of respiratory symptoms from june 2008 to may 2009 were ... | 2011 | 21829870 |
| a multi-tiered time-series modelling approach to forecasting respiratory syncytial virus incidence at the local level. | summaryrespiratory syncytial virus (rsv) is the most common cause of documented viral respiratory infections, and the leading cause of hospitalization, in young children. we performed a retrospective time-series analysis of all patients aged <18 years with laboratory-confirmed rsv within a network of multiple affiliated academic medical institutions. forecasting models of weekly rsv incidence for the local community, inpatient paediatric hospital and paediatric intensive-care unit (picu) were cr ... | 2011 | 21676348 |
| il-17-induced pulmonary pathogenesis during respiratory viral infection and exacerbation of allergic disease. | severe respiratory syncytial virus (rsv) infections are characterized by airway epithelial cell damage, mucus hypersecretion, and th2 cytokine production. less is known about the role of il-17. we observed increased il-6 and il-17 levels in tracheal aspirate samples from severely ill infants with rsv infection. in a mouse model of rsv infection, time-dependent increases in pulmonary il-6, il-23, and il-17 expression were observed. neutralization of il-17 during infection and observations from il ... | 2011 | 21703407 |
| respiratory syncytial virus-associated hospitalizations among infants and young children in the united states, 1997-2006. | background:: respiratory syncytial virus (rsv) is the most common cause of lower respiratory tract disease among young children in the united states. rsv-associated hospitalization increased among children in the united states during 1980 through 1996. in this study, we updated national estimates of rsv hospitalization rates among us children through 2006. methods:: we conducted a retrospective analysis of hospital discharges for lower respiratory tract illness (lrti) in children <5 years old fr ... | 2011 | 21817948 |
| human metapneumovirus in patients with respiratory tract infection in kuwait. | human metapneumovirus (hmpv) has been recognized as an important cause of respiratory tract infections in all age groups and in all geographical area. the role of hmpv in causing respiratory tract infections in kuwait was not yet investigated. the aim of this study was to determine the prevalence of hmpv infection in kuwait among patients with respiratory tract infection with respect to other respiratory viruses. during january-december 2009, 460 respiratory samples from 388 patients with respir ... | 2011 | 21837799 |
| bioengineering rna silencing across the life kingdoms. | rna silencing negatively regulates gene expression at transcriptional and posttranscriptional levels, guided by small rna molecules. it modulates core regulatory pathways across the eukaryotes, such as developmental processes or stress responses. the widespread existence of this phenomenon and the key pathways regulated have led to the development of a new technology based on the modification of gene expression, which has been applied successfully in different areas such as medicine or agricultu ... | 2011 | 21707526 |
| self-collected mid-turbinate swabs for the detection of respiratory viruses in adults with acute respiratory illnesses. | the gold standard for respiratory virus testing is a nasopharyngeal (np) swab, which is collected by a healthcare worker. midturbinate (mt) swabs are an alternative due to their ease of collection and possible self-collection by patients. the objective of this study was to compare the respiratory virus isolation of flocked mt swabs compared to flocked np swabs. | 2011 | 21731708 |
| type-iii interferon, not type-i, is the predominant interferon induced by respiratory viruses in nasal epithelial cells. | as an innate immune response against diverse viral infections, a host induces two types of interferon (ifn), type-i (ifn-+¦/+¦) and type-iii (ifn-++). we investigated ifn inductions by respiratory viruses, including respiratory syncytial virus (rsv), measles virus and mumps virus in human nasal epithelial cells (necs). ifn-++, but not ifn-+¦/+¦, was induced by respiratory virus infection in primary necs and immortalized necs through transfection with the human telomerase reverse transcriptase ge ... | 2011 | 21816185 |
| generation of recombinant metapneumovirus nucleocapsid protein as nucleocapsid-like particles and development of virus-specific monoclonal antibodies. | human metapneumovirus (hmpv) is a member of the pneumovirinea subfamily within the paramyxoviridea family. since its discovery in 2001, hmpv has been isolated in several continents, which suggests its prevalence worldwide. hmpv resembles human respiratory syncytial virus with regard to disease symptoms and its ability to infect and cause disease in young infants as well as individuals of all ages. the aim of the current study was to construct an efficient high-level yeast expression system for t ... | 2011 | 21827798 |
| systemic signature of the lung response to respiratory syncytial virus infection. | respiratory syncytial virus is a frequent cause of severe bronchiolitis in children. to improve our understanding of systemic host responses to rsv, we compared balb/c mouse gene expression responses at day 1, 2, and 5 during primary rsv infection in lung, bronchial lymph nodes, and blood. we identified a set of 53 interferon-associated and innate immunity genes that give correlated responses in all three murine tissues. additionally, we identified blood gene signatures that are indicative of ac ... | 2011 | 21731757 |
| paediatric intensive care admissions for respiratory syncytial virus bronchiolitis in france: results of a retrospective survey and evaluation of the validity of a medical information system programme. | summarythe purpose of this study was to describe the characteristics of patients with bronchiolitis admitted to a paediatric intensive care unit (picu), and to evaluate a national registry of hospitalizations (programme de m+®dicalisation des syst+¿mes d'information; pmsi) as a potential source of epidemiological data. of the 49 french picus invited to take part in a retrospective survey of children aged <2 years who were hospitalized during the 2005-2006 epidemic season, 24 agreed to participat ... | 2011 | 21733254 |
| identification of nucleolin as a cellular receptor for human respiratory syncytial virus. | human respiratory syncytial virus (rsv) causes a large burden of disease worldwide. there is no effective vaccine or therapy, and the use of passive immunoprophylaxis with rsv-specific antibodies is limited to high-risk patients. the cellular receptor (or receptors) required for viral entry and replication has yet to be described; its identification will improve understanding of the pathogenesis of infection and provide a target for the development of novel antiviral interventions. here we show ... | 2011 | 21841784 |
| tak1 regulates nf-+üb and ap-1 activation in airway epithelial cells following rsv infection. | respiratory syncytial virus (rsv) is the most common cause of epidemic respiratory diseases in infants and young children. rsv infection of airway epithelial cells induces the expression of immune/inflammatory genes through the activation of a subset of transcription factors, including nuclear factor-+¦b (nf-+¦b) and ap-1. in this study, we have investigated the signaling pathway leading to activation of these two transcription factors in response to rsv infection. our results show that ikk+¦ pl ... | 2011 | 21835421 |
| respiratory viruses involved in influenza-like illness in a greek pediatric population during the winter period of the years 2005-2008. | viruses are the major cause of pediatric respiratory tract infection and yet many suspected cases of illness remain uncharacterized. this study aimed to determine the distribution of several respiratory viruses in children diagnosed as having influenza-like illness, over the winter period of 2005-2008. molecular assays including conventional and real time pcr protocols, were employed to screen respiratory specimens, collected by clinicians of the influenza sentinel system and of outpatient pedia ... | 2011 | 21837803 |
| decreased innate immune cytokine responses correlate with disease severity in children with respiratory syncytial virus and human rhinovirus bronchiolitis. | the immunopathogenesis of respiratory syncytial virus (rsv) and human rhinovirus lower respiratory tract infections in children remains to be defined. we measured nasal wash concentrations of 29 cytokines in infants with rsv or human rhinovirus lower respiratory tract infections. concentrations of interferon-+¦ in rsv and innate immunity cytokines in both infections inversely correlated with disease severity. | 2011 | 21829141 |
| real-world comparison of two molecular methods for detection of respiratory viruses. | abstract: | 2011 | 21714915 |
| prevalence of bordetella pertussis and bordetella parapertussis in infants presenting to the emergency department with bronchiolitis. | the clinical presentation of bordetella pertussis can overlap with that of respiratory syncytial virus (rsv), and coinfection does occur, but management differs. | 2011 | 19062227 |
| the next ten stories on antiviral drug discovery (part e): advents, advances, and adventures. | this review article presents the fifth part (part e) in the series of stories on antiviral drug discovery. the ten stories belonging to this fifth part are dealing with (i) aurintricarboxylic acid; (ii) alkenyldiarylmethanes; (iii) human immunodeficiency virus (hiv) integrase inhibitors; (iv) lens epithelium-derived growth factor as a potential target for hiv proviral dna integration; (v) the status presens of neuraminidase inhibitors nais in the control of influenza virus infections; (vi) the s ... | 2011 | 19844936 |
| acute phase protein changes in calves during an outbreak of respiratory disease caused by bovine respiratory syncytial virus. | bovine acute phase proteins (apps), lipopolysaccharide binding protein (lbp), serum amyloid a (saa), haptoglobin (hp) and alpha(1)-acid glycoprotein (agp) were evaluated as inflammatory markers during an outbreak of bovine respiratory disease (brd) caused by bovine respiratory syncytial virus (brsv). calves (n = 10) presented mild to moderate signs of respiratory disease. secondary bacterial infections, pasteurella multocida and mycoplasma dispar as major species, were detected in tracheobronchi ... | 2011 | 19897247 |
| genetic diversity in the g protein gene of human respiratory syncytial virus among iranian children with acute respiratory symptoms. | acute respiratory infection (ari) is the major cause of morbidity and mortality in children worldwide. human respiratory syncytial virus (hrsv) is main viral agent of ari in infants and young children in terms of effect and prevalence. the aim of this study was to investigate hrsv genotypes during one season in iran. | 2011 | 23056765 |
| structural analysis of human respiratory syncytial virus p protein: identification of intrinsically disordered domains. | human respiratory syncytial virus p protein plus the viral rna, n and l viral proteins, constitute the viral replication complex. in this report we describe that hrsv p protein has putative intrinsically disordered domains predicted by in silico methods. these two domains, located at the amino and caboxi terminus, were identified by mass spectrometry analysis of peptides obtained from degradation fragments observed in purified p protein expressed in bacteria. the degradation is not occurring at ... | 2011 | 24031640 |
| prevalence of upper respiratory tract infections at a tertiary care hospital in the city of são paulo. | to evaluate the prevalence of pathogens in the upper respiratory tract according to age at a tertiary care hospital in the city of são paulo. | 2010 | 26760003 |
| first report of respiratory syncytial virus and human metapneumovirus co-infection in a 2-year-old kawasaki patient in iran. | respiratory virus infections in children are a leading cause of morbidity and mortality worldwide. | 2010 | 23113048 |
| human metapneumovirus and respiratory syncytial virus: subtle differences but comparable severity. | human metapneumovirus (hmpv) is a recently discovered virus that causes respiratory illness in children that can lead to hospitalization. our study was undertaken to further understand hmpv-associated illness, compare clinical characteristics of hmpv and respiratory syncytial virus (rsv), and establish the utility of routine screening for hmpv. we retrospectively identified hmpv-associated illnesses described among children with respiratory symptoms admitted to a tertiary care center in southeas ... | 2010 | 24470892 |
| newcastle disease virus-like particles containing respiratory syncytial virus g protein induced protection in balb/c mice, with no evidence of immunopathology. | respiratory syncytial virus (rsv) is the leading cause of serious respiratory infections in children as well as a serious cause of disease in elderly and immunosuppressed populations. there are no licensed vaccines available to prevent rsv disease. we have developed a virus-like particle (vlp) vaccine candidate for protection from rsv. the vlp is composed of the np and m proteins of newcastle disease virus (ndv) and a chimeric protein containing the cytoplasmic and transmembrane domains of the n ... | 2010 | 19889768 |
| vaccination to induce antibodies blocking the cx3c-cx3cr1 interaction of respiratory syncytial virus g protein reduces pulmonary inflammation and virus replication in mice. | respiratory syncytial virus (rsv) infection causes substantial morbidity and some deaths in the young and elderly worldwide. there is no safe and effective vaccine available, although it is possible to reduce the hospitalization rate for high-risk children by anti-rsv antibody prophylaxis. rsv has been shown to modify the immune response to infection, a feature linked in part to rsv g protein cx3c chemokine mimicry. this study determined if vaccination with g protein polypeptides or peptides spa ... | 2010 | 19864390 |
| intranasal immunization of mice with a bovine respiratory syncytial virus vaccine induces superior immunity and protection compared to those by subcutaneous delivery or combinations of intranasal and subcutaneous prime-boost strategies. | bovine respiratory syncytial virus (brsv) infects cells of the respiratory mucosa, so it is desirable to develop a vaccination strategy that induces mucosal immunity. to achieve this, various delivery routes were compared for formalin-inactivated (fi) brsv formulated with cpg oligodeoxynucleotide (odn) and polyphosphazene (pp). intranasal delivery of the fi-brsv formulation was superior to subcutaneous delivery in terms of antibody, cell-mediated, and mucosal immune responses, as well as reducti ... | 2010 | 19864487 |
| respiratory syncytial virus infection of camels (camelus dromedaries). | this study aimed to investigate the occurrence of respiratory syncytial virus (rsv) infections in camels in sudan. a total of 272 camel lung specimens showing pneumonia were collected from slaughter houses at four different areas in sudan, additionally 8 specimens were collected from outbreaks of respiratory infection in camels. using sandwich elisa kits for rsv antigen detection 4 out of 280 tested lungs (1.4%) were positive, all were from central sudan (tambool slaughter house). fat was used t ... | 2010 | 19840769 |
| haplotype of il-8 -251t and 781c is associated with the susceptibility to respiratory syncytial virus. | to study the association of haplotypes of il-8 -251t/a and 781 c/t single nucleotide polymorphisms (snps) with the susceptibility of respiratory syncytial virus (rsv). | 2010 | 19887538 |
| helmet-delivered continuous positive airway pressure with heliox in respiratory syncytial virus bronchiolitis. | the objective of this study was to check the feasibility and efficacy of helmet-delivered heliox-continuous positive airway pressure (cpap) in infants with bronchiolitis. | 2010 | 19811455 |
| low incidence of severe respiratory syncytial virus infections in lung transplant recipients despite the absence of specific therapy. | respiratory syncytial virus (rsv) infections in lung transplant recipients (ltrs) have been associated with significant morbidity and mortality. immunoglobulins, ribavirin, and palivizumab are suggested treatments for both pre-emptive and therapeutic purposes. however, in the absence of randomized, placebo-controlled trials, efficacy is controversial and there is toxicity as well as cost concerns. | 2010 | 19837611 |
| empiric antibiotics are justified for infants with respiratory syncytial virus lower respiratory tract infection presenting with respiratory failure: a prospective study and evidence review. | although some studies indicate a low risk of serious bacterial infection in infants with respiratory syncytial virus (rsv), these studies focused on patients who did not progress to respiratory failure. we hypothesized the composite diagnosis of concomitant bacterial pneumonia (cbp) is common in lower risk infants with rsv who present in respiratory failure. the aim of the study was to investigate the incidence of cbp in low-risk infants mechanically ventilated for rsv respiratory failure and to ... | 2010 | 19838143 |
| probabilistic indoor transmission modeling for influenza (sub)type viruses. | to use a probability based transmission modeling approach to examine the influenza risk of infection virus in indoor environments. this was based on 10 years of data gathered from influenza-like illness sentinel physician and laboratory surveillance, and experimental viral shedding data in taiwan. | 2010 | 19818365 |
| respiratory syncytial virus, human bocavirus and rhinovirus bronchiolitis in infants. | to investigate the prevalence of 14 viruses in infants with bronchiolitis and to study demographic and clinical differences in those with respiratory syncytial virus (rsv), human bocavirus (hbov) and rhinovirus (rv) infection. | 2010 | 19822538 |
| viral pathogens associated with acute respiratory infections in central vietnamese children. | hospitalized vietnamese children with acute respiratory infection were investigated for 13 viral pathogens using multiplex-polymerase chain reaction. we enrolled 958 children of whom 659 (69%) had documented viral infection: rhinovirus (28%), respiratory syncytial virus (23%), influenza virus (15%), adenovirus (5%), human metapneumo virus (4.5%), parainfluenza virus (5%), and bocavirus (2%). these vietnamese children had a range of respiratory viruses which underscores the need for enhanced acut ... | 2010 | 19907358 |
| high incidence of recurrent wheeze in children with down syndrome with and without previous respiratory syncytial virus lower respiratory tract infection. | respiratory syncytial virus (rsv)-induced lower respiratory tract infection (lrti) is associated with the subsequent development of recurrent wheeze. in a recent study, we found a high incidence (9.9%) of hospitalization for rsv-induced lrti among children with down syndrome (ds), indicating ds as a new risk factor for rsv-induced lrti. in the current study we aimed to investigate the development of long-term airway morbidity in children with ds after hospitalization for rsv-induced lrti. | 2010 | 19907362 |
| surfactant protein a2 polymorphisms and disease severity in a respiratory syncytial virus-infected population. | to examine whether genetic variations within the surfactant protein a2 (sp-a2) gene are associated with respiratory syncytial virus (rsv) disease severity in infected children. | 2010 | 19914637 |
| impact of palivizumab on rsv hospitalizations for children with hemodynamically significant congenital heart disease. | the objective of this study was to evaluate the impact of palivizumab prophylaxis on respiratory syncytial virus (rsv) hospitalizations among children with hemodynamically significant congenital heart disease (chd). in 2003, the american academy of pediatrics (aap) revised the bronchiolitis policy statement and recommended the use of palivizumab in children <24 months old with hemodynamically significant chd (hs-chd). california statewide hospital discharge data from years 2000-2002 (pre-aap pol ... | 2010 | 19915892 |
| bovine respiratory syncytial virus seroprevalence and risk factors in endemic dairy cattle herds. | the herd seroprevalence of bovine respiratory syncytial virus (brsv) was studied in 59 dairy cattle herds using serology on random selected animals stratified by two age classes (heifers, cows). risk factors for primary infections in heifers were investigated using a questionnaire on management conditions and data on bovine viral diarrhoea (bvd) status. at least one seropositive cow was present in all the herds. in 25% of the herds all individual were seropositive and 22% of herds had all heifer ... | 2010 | 19921456 |
| elevated temperature triggers human respiratory syncytial virus f protein six-helix bundle formation. | human respiratory syncytial virus (rsv) is a major cause of severe lower respiratory tract infection in infants, immunocompromised patients, and the elderly. the rsv fusion (f) protein mediates fusion of the viral envelope with the target cell membrane during virus entry and is a primary target for antiviral drug and vaccine development. the f protein contains two heptad repeat regions, hr1 and hr2. peptides corresponding to these regions form a six-helix bundle structure that is thought to play ... | 2010 | 19922971 |
| virological diagnosis of respiratory virus infection in patients attending an emergency department during the influenza season. | to investigate the burden of influenza-like illness (ili), patients attending an emergency department during the influenza season were tested for several common respiratory viruses, using pcr-based methods. influenza a viruses were detected in 25 of 103 recruited patients (24%), rhinoviruses in 15%, and respiratory syncytial virus in only one. the data suggest that triage criteria based on ili case definitions would not contain the spread of the influenza virus during pandemic alerts and could l ... | 2010 | 19925480 |
| treatment of human respiratory syncytial virus infected balb/c mice with the proteasome inhibitor bortezomib (velcade, ps-341) results in increased inflammation and mortality. | human respiratory syncytial virus (hrsv) is an important pathogen and is associated with mortality in the young, old, and immuno-compromised. due to the lack of effective therapeutic antivirals or a vaccine, there is a critical need for continued research in this field. here we tested the ability of the fda approved proteasome inhibitor bortezomib to inhibit hrsv in vitro and in vivo. we observed significant inhibition of hrsv replication in vero cells at bortezomib concentrations from 20 to 40 ... | 2010 | 19931343 |
| [epidemiological and clinical description of human metapneumovirus infectious diseases in children]. | human metapneumovirus (hmpv), a recently identified respiratory virus, is a leading cause of acute respiratory tract infection in children during winter. the aims of this study were to outline epidemiological and clinical presentations of hmpv infectious diseases in young children. | 2010 | 19932012 |
| achieving control of asthma in preschoolers. | 2010 | 19933790 | |
| sole pathogen in acute bronchiolitis: is there a role for other organisms apart from respiratory syncytial virus? | acute bronchiolitis (ab) is a common disease of young children with peak incidence during the winter season. respiratory syncytial virus (rsv) is a major causative organism, yet recent relatively small sized studies have suggested an increased role of other organisms as sole or codetected organisms. the aim of this study was to assess the prevalence of sole- and mixed-organisms infections in hospitalized children with ab, using combined antigen-based and polymerase chain reaction assays (pcr). | 2010 | 19935450 |
| clinical presentation and severity of viral community-acquired pneumonia in young nepalese children. | most deaths from pneumonia in children <5 years of age occur in developing countries, where information about the clinical impact and severity of viral causes of respiratory infections is limited. | 2010 | 19935451 |
| influenza-attributable mortality in australians aged more than 50 years: a comparison of different modelling approaches. | this study aimed to compare systematically approaches to estimating influenza-attributable mortality in older australians. using monthly age-specific death data together with viral surveillance counts for influenza and respiratory syncytial virus, we explored two of the most frequently used methods of estimating excess influenza-attributable disease: poisson and serfling regression models. these approaches produced consistent age and temporal patterns in estimates of influenza-attributable morta ... | 2010 | 19941685 |
| respiratory syncytial virus infections in central australia. | little is known about the epidemiology of respiratory syncytial virus (rsv) infection in arid desert regions and in the aboriginal population. we describe the seasonality and epidemiology of rsv infection in central australia, an arid area with a large aboriginal population. | 2010 | 19943864 |
| intranasal vaccination with a helper-dependent adenoviral vector enhances transgene-specific immune responses in balb/c mice. | helper-dependent adenoviral (hdad) vectors were developed primarily for genetic disease therapy by deleting all coding regions for attenuating the host cellular immune response to adenovirus (ad) and long-lasting gene expression. recently harui et al. reported that hdad vaccine could stimulate superior transgene-specific cytotoxic t lymphocyte (ctl) and antibody responses via the intraperitoneal route, compared to first-generation adenoviral (fgad) vaccine. this prompted us to explore the potent ... | 2010 | 19945423 |
| ki polyomavirus detected in respiratory tract specimens from patients in st. louis, missouri. | studies have reported the presence of ki polyomavirus (kipyv) and wu polyomavirus (wupyv) in respiratory secretions of young patients. so far, evidence has not supported a link between infections with either virus and respiratory tract disease; however, there has not been a large comparison of kipyv-infected patients to age-matched patient groups. | 2010 | 19949359 |
| [moraxella catarrhalis sepsis in a 4 month-old infant with rsv+ bronchiolitis]. | 2010 | 19955027 | |
| development of a rapid automated influenza a, influenza b, and respiratory syncytial virus a/b multiplex real-time rt-pcr assay and its use during the 2009 h1n1 swine-origin influenza virus epidemic in milwaukee, wisconsin. | rapid, semiautomated, and fully automated multiplex real-time rt-pcr assays were developed and validated for the detection of influenza (flu) a, flu b, and respiratory syncytial virus (rsv) from nasopharyngeal specimens. the assays can detect human h1n1, h3n2, and swine-origin (s-oiv) h1n1 flu a viruses and were effectively used to distinguish flu a infections (of all subtypes) from flu b and rsv infections during the current s-oiv outbreak in milwaukee, wi. the analytical limits of detection we ... | 2010 | 19959800 |
| serologically verified human bocavirus pneumonia in children. | working hypothesis: human bocavirus (hbov) is a newly identified parvovirus frequently found in children suffering from acute respiratory and intestinal infections. the aim of the present study was to evaluate, by using a newly developed antibody assay, the role of hbov in pediatric community-acquired pneumonia (cap) and the seropositivity rate to hbov in a prospective study in north-italian children. materials/patients and methods: during a 15-month study period, 124 children were admitted due ... | 2010 | 19960524 |
| respiratory syncytial virus induces airway insensitivity to beta-agonists in balb/c mice. | beta-adrenergic agonists (beta-agonists) are commonly used to treat respiratory syncytial virus (rsv) bronchiolitis but are generally ineffective for unknown reasons. we have previously shown that rsv strain a2 inhibits bronchoalveolar epithelial responses to beta-agonists in a balb/c mouse model by inducing heterologous keratinocyte cytokine (kc)/cxcr2-mediated desensitization of epithelial beta(2)-adrenergic receptors. the aim of the current study was to determine whether rsv also induces airw ... | 2010 | 19965982 |
| binding of a potent small-molecule inhibitor of six-helix bundle formation requires interactions with both heptad-repeats of the rsv fusion protein. | six-helix bundle (6hb) formation is an essential step for many viruses that rely on a class i fusion protein to enter a target cell and initiate replication. because the binding modes of small molecule inhibitors of 6hb formation are largely unknown, precisely how they disrupt 6hb formation remains unclear, and structure-based design of improved inhibitors is thus seriously hampered. here we present the high resolution crystal structure of tmc353121, a potent inhibitor of respiratory syncytial v ... | 2010 | 19966279 |
| cost effectiveness of palivizumab in spain: an analysis using observational data. | to assess the cost effectiveness of palivizumab for prevention of severe respiratory syncytial virus (rsv) disease in high-risk infants in spain, incorporating country-specific observational hospitalisation data. | 2010 | 19967425 |
| the interleukin 6 -174 c/c genotype predicts greater rhinovirus illness. | in adults and children with respiratory syncytial virus (rsv) infection, a polymorphism in the interleukin 6 (il-6) promoter at position -174 predicts illness magnitude. in addition, polymorphisms in the interleukin 10 (il-10), tumor necrosis factor alpha (tnf-alpha), and interferon gamma (ifn-gamma) genes are associated with immune responsiveness and the frequency of complications. here, the effect of these polymorphisms on illness and seroconversion during infection with rhinovirus type 39 (rv ... | 2010 | 20001857 |
| increased prevalence of otitis media following respiratory syncytial virus infection. | the aim of this study was to analyse whether, during the 18 months following a respiratory syncytial virus (rsv) infection in infants, there were differences in the prevalence of common infections such as acute otitis media (aom), compared with controls. we also wanted to see whether passive smoking could be a contributory factor. | 2010 | 20002623 |
| mathematical modelling of respiratory syncytial virus (rsv): vaccination strategies and budget applications. | we propose an age-structured mathematical model for respiratory syncytial virus in which children aged <1 year are especially considered. real data on hospitalized children in the spanish region of valencia were used in order to determine some seasonal parameters of the model. weekly predictions of the number of children aged <1 year that will be hospitalized in the following years in valencia are presented using this model. results are applied to estimate the regional cost of paediatric hospita ... | 2010 | 20003640 |
| implementation of real-time rt-pcr for detection of human metapneumovirus and its comparison with enzyme immunoassay. | human metapneumovirus (hmpv) is responsible for outbreaks of bronchiolitis in winter and early spring in young children. due to the relatively recent discovery of hmpv, the diagnostic opportunities are limited, while differential diagnosis with respiratory syncytial virus (rsv) remains important. we validated the rt-pcr by comparing various methods of rna extraction, one-step rt-pcr kits and primer-probe combinations. the optimized rt-pcr was evaluated using 47 nasopharyngeal aspirates (npas) co ... | 2010 | 20013132 |
| a systemic neutrophil response precedes robust cd8(+) t-cell activation during natural respiratory syncytial virus infection in infants. | severe primary respiratory syncytial virus (rsv) infections are characterized by bronchiolitis accompanied by wheezing. controversy exists as to whether infants suffer from virus-induced lung pathology or from excessive immune responses. furthermore, detailed knowledge about the development of primary t-cell responses to viral infections in infants is lacking. we studied the dynamics of innate neutrophil and adaptive t-cell responses in peripheral blood in relation to the viral load and paramete ... | 2010 | 20015982 |
| genomewide association analysis of respiratory syncytial virus infection in mice. | respiratory syncytial virus (rsv) is the major cause of lower respiratory tract infection in infants, with about half being infected in their first year of life. yet only 2 to 3% of infants are hospitalized for rsv infection, suggesting that individual susceptibility contributes to disease severity. previously, we determined that akr/j (susceptible) mice developed high lung rsv titers and showed delayed weight recovery, whereas c57bl/6j (resistant) mice demonstrated low lung rsv titers and rapid ... | 2010 | 20015999 |
| population based external validation of a european predictive model for respiratory syncytial virus hospitalization of premature infants born 33 to 35 weeks of gestational age. | prospectively collected population-based data on 2529 danish infants born at 33 to 35 weeks of gestation were used to validate an european predictive model of respiratory syncytial virus (rsv) hospitalization. the model was found to be robust with a diagnostic accuracy of 65.9% to distinguish between rsv-hospitalized versus non-rsv-hospitalized danish infants born at 33 to 35 weeks of gestation. | 2010 | 20016397 |
| granzyme a- and b-cluster deficiency delays acute lung injury in pneumovirus-infected mice. | lower respiratory tract infection by the human pneumovirus respiratory syncytial virus is a frequent cause of acute lung injury in children. severe pneumovirus disease in humans is associated with activation of the granzyme pathway by effector lymphocytes, which may promote pathology by exaggerating proapoptotic caspase activity and proinflammatory activity. the main goal of this study was to determine whether granzymes contribute to the development of acute lung injury in pneumovirus-infected m ... | 2010 | 20018616 |
| role of rapid immunochromatographic antigen testing in diagnosis of influenza a virus 2009 h1n1 infection. | rapid antigen testing using immunochromatographic devices has become a diagnostic mainstay for detection of influenza virus and respiratory syncytial virus, the two major viruses infecting the respiratory tract. recent studies have indicated that poor performance in the detection of the novel influenza a virus 2009 h1n1 should preclude their use. a survey of influenza diagnostic methods available on clinmicronet and division c, the two asm list servers, revealed that, despite this reported poor ... | 2010 | 20007399 |
| vitamin d decreases respiratory syncytial virus induction of nf-kappab-linked chemokines and cytokines in airway epithelium while maintaining the antiviral state. | epidemiological studies suggest that low vitamin d levels may increase the risk or severity of respiratory viral infections. in this study, we examined the effect of vitamin d on respiratory syncytial virus (rsv)-infected human airway epithelial cells. airway epithelium converts 25-hydroxyvitamin d3 (storage form) to 1,25-dihydroxyvitamin d3 (active form). active vitamin d, generated locally in tissues, is important for the nonskeletal actions of vitamin d, including its effects on immune respon ... | 2010 | 20008294 |
| motavizumab for prophylaxis of respiratory syncytial virus in high-risk children: a noninferiority trial. | palivizumab reduces respiratory syncytial virus (rsv) hospitalization in children at high risk by approximately 50% compared with placebo. we compared the efficacy and safety of motavizumab, an investigational monoclonal antibody with enhanced anti-rsv activity in preclinical studies, with palivizumab. | 2010 | 20008423 |
| phosphorylation of paramyxovirus phosphoprotein and its role in viral gene expression. | paramyxoviruses include many important human and animal pathogens such as measles virus, mumps virus, human parainfluenza viruses, and respiratory syncytial virus, as well as emerging viruses such as nipah virus and hendra virus. the paramyxovirus rna-dependent rna polymerase consists of the phosphoprotein (p) and the large protein. both of these proteins are essential for viral rna synthesis. the p protein is phosphorylated at multiple sites, probably by more than one host kinase. while it is t ... | 2010 | 20020826 |
| generation of stable monoclonal antibody-producing b cell receptor-positive human memory b cells by genetic programming. | the b cell lymphoma-6 (bcl-6) and bcl-xl proteins are expressed in germinal center b cells and enable them to endure the proliferative and mutagenic environment of the germinal center. by introducing these genes into peripheral blood memory b cells and culturing these cells with two factors produced by follicular helper t cells, cd40 ligand (cd40l) and interleukin-21 (il-21), we convert them to highly proliferating, cell surface b cell receptor (bcr)-positive, immunoglobulin-secreting b cells wi ... | 2010 | 20023635 |
| human bocavirus infection in young children with acute respiratory tract infection in lanzhou, china. | human bocavirus (hbov) is a recognized human parvovirus associated with acute respiratory tract infection. however, hbov has yet to be established as a causative agent of respiratory disease. in this study, the epidemiological and virological characteristics of hbov infection were studied in children with acute respiratory tract infection in china. in total, 406 children younger than 14 years of age with acute respiratory tract infection were included in this prospective 1-year study. hbov was d ... | 2010 | 20029808 |
| kawasaki disease patients with redness or crust formation at the bacille calmette-guérin inoculation site. | a specific diagnostic test for kawasaki disease (kd) is currently unavailable. redness or crust formation at the bacille calmette-guérin (bcg) inoculation site is listed as a positive sign in the diagnostic guidelines of kd. the purpose of this study was to investigate the epidemiologic features of kd patients with such changes at the bcg inoculation site and to evaluate the specificity of this sign in kd diagnosis. | 2010 | 20032807 |
| respiratory syncytial virus market. | 2010 | 20043025 | |
| description of the outcomes of prior authorization of palivizumab for prevention of respiratory syncytial virus infection in a managed care organization. | respiratory syncytial virus (rsv) is the leading cause of upper and lower respiratory tract infections in infants and young children. most children are exposed to the virus before they are 2 years old and experience such symptoms as cough, fever, and irritability. in a select population of infants, the virus can cause hypoxemia and hospitalization. to avoid hospitalization, good infection control practices should be employed, and for those infants at high risk, prophylaxis with palivizumab is in ... | 2010 | 20044843 |
| medical utilization associated with palivizumab compliance in a commercial and managed medicaid health plan. | respiratory syncytial virus (rsv) is the leading cause of lower respiratory tract infections and hospitalization in infants. palivizumab is currently the only available agent for prevention of rsv infection in high-risk infants. this high-cost injectable requires monthly dosing during the rsv season. compliance with the injection schedule is important in the prevention of rsv infection and respiratory complications. managed care organizations have an interest in the relationship between complian ... | 2010 | 20044844 |
| utilization management opportunities for palivizumab for prophylaxis of respiratory syncytial virus complications in infants. | 2010 | 20044846 | |
| role of surfactant protein a and d (sp-a and sp-d) in human antiviral host defense. | sp-a and sp-d contribute to host defense against respiratory viral infection. the most extensive body of evidence relates to influenza a viruses (iav), and evidence from gene-deleted mice also indicate a role for surfactant collectins in defense against respiratory syncytial virus (rsv) and adenovirus. some important respiratory pathogens including rhinovirus and metapneumovirus have not yet been examined. viral pathogens that enter the body via the respiratory tract (e.g., ebola virus), replica ... | 2010 | 20036966 |
| activation of interferon response through toll-like receptor 3 impacts viral pathogenesis and pulmonary toll-like receptor expression during respiratory syncytial virus and influenza infections in the cotton rat sigmodon hispidus model. | interferon (ifn) therapy in humans often causes flu-like symptoms by an unknown mechanism. poly iclc is a synthetic dsrna and a toll-like receptor 3 (tlr3) agonist with a strong ifn-inducing ability. in this work, we analyzed the effect of poly iclc on pulmonary responses to influenza and respiratory syncytial virus (rsv) infections in the cotton rat (sigmodon hispidus) model. viral replication, pulmonary inflammation, and expression of ifn, tlr, and chemokines were monitored and compared. antiv ... | 2010 | 20038196 |
| double-stranded rna-activated protein kinase regulates early innate immune responses during respiratory syncytial virus infection. | respiratory syncytial virus (rsv) is the most common cause of childhood viral bronchiolitis and lung injury. inflammatory responses significantly contribute to lung pathologies during rsv infections and bronchiolitis but the exact mechanisms have not been completely defined. the double-stranded rna-activated protein kinase (pkr) functions to inhibit viral replication and participates in several signaling pathways associated with innate inflammatory immune responses. using a functionally defectiv ... | 2010 | 20038207 |
| pulmonary infection of mice with human metapneumovirus induces local cytotoxic t-cell and immunoregulatory cytokine responses similar to those seen with human respiratory syncytial virus. | human metapneumovirus (hmpv) is a major cause of upper and lower respiratory-tract infection in infants, the elderly and immunocompromised individuals. virus-directed cellular immunity elicited by hmpv infection is poorly understood, in contrast to the phylogenetically and clinically related pathogen human respiratory syncytial virus (hrsv). in a murine model of acute lower respiratory-tract infection with hmpv, we demonstrate the accumulation of gamma interferon (ifn-gamma)-producing cd8+ t cel ... | 2010 | 20053825 |
| evaluation of multiplex type-specific real-time pcr assays using the lightcycler and joint biological agent identification and diagnostic system platforms for detection and quantitation of adult human respiratory adenoviruses. | every year, thousands of basic military trainees in each service of the u.s. armed forces experience acute respiratory disease. the majority of this disease burden results from infection with human adenoviruses. we designed single- and multiplex assays that detect and discriminate adenovirus types b3, e4, b7, b11, b14, and b21. a total of 116 oropharyngeal swab specimens obtained from patients at the naval health research center were used to validate the new assays. type-specific singleplex assa ... | 2010 | 20053853 |