Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| peripheral blood t and b lymphocyte subpopulations in infants with acute respiratory syncytial virus brochiolitis. | most data concerning immunopathogenetic mechanisms involved in respiratory syncytial virus (rsv) infection are derived from animal studies. in infants with rsv bronchiolitis the target organ i.e. the airway is hard to explore. we looked for specific alterations in peripheral blood lymphocyte subpopulations in infants hospitalized for rsv bronchiolitis. flow cytometric analysis with a large panel of monoclonals was performed on peripheral blood lymphocytes in thirty-two infants (mean age: 4.9 mon ... | 1997 | 9617780 |
| molecular basis of the altered gag p19 protein (ma) of the transformation-defective mutant of rous sarcoma virus, tdph2010. | the transformation-defective mutant of rous sarcoma virus (rsv), tdph2010, has a gag p19 (matrix; ma) protein which migrates on sds-polyacrylamide gels faster than that of the parental (tsny68) as well as other wild-type strains of rsv. to study the molecular basis of this altered migration pattern and its biological significance, the nucleotide sequence of the p19 region of tdph2010 was determined. comparison of the nucleotide sequence of tdph2010 with that of the schmidt-ruppin a strain of rsv ... | 1997 | 9595258 |
| production of triploid transgenic zebrafish, brachydanio rerio (hamilton). | eggs of zebrafish brachydanio rerio were heat-shocked just after fertilisation to induce triploidy and subsequently subjected to microinjection with the linear recombinant plasmid containing the rous sarcoma virus-long terminal repeat (rsv-ltr) promoter linked to the rainbow trout (oncorhynchus mykiss) growth hormone (gh)cdna(prsvrtgh). slot and southern analyses showed the extra chromosomal transfer of prsvrtgh. expression of prsvrtgh was detected in the triploid transformants by rna slot, nort ... | 1997 | 9567756 |
| [breast feeding for prevention of viral acute respiratory diseases in infants]. | the incidence of acute viral respiratory diseases (confirmed by immunofluorescence test) was compared in a group of 159 infants aged 0-9 months breast-fed at least once daily and in a group of 608 infants of similar age and hospitalization duration receiving only artificial food. the incidence was significantly lower in breast-fed infants (p = 0.00057), especially in the first trimester of life (p = 0.00039). this was valid of all rsv infections (p = 0.01854) and parainfluenza type 4 virus infec ... | 1997 | 9554153 |
| [etiology of acute lower respiratory tract infections among children younger than 5 years old in santa fe]. | the etiology of acute lower respiratory tract infections (ari) and nasopharyngeal bacterial carriage in children less than 5 years old living in santa fe city, argentina, was studied. a total of 518 children were included in the study: 450 suffering from ari and 68 asymptomatic children. blood samples, pleural effusions and nasopharyngeal secretions (ns) were obtained from children for bacterial isolations. ns was also used for fluorescent antibody techniques, and serum samples were employed for ... | 1997 | 9532829 |
| cd8+ t cells control th2-driven pathology during pulmonary respiratory syncytial virus infection. | balb/c mice vaccinated with vaccinia virus expressing the major surface glycoprotein g of respiratory syncytial virus (rsv) develop lung eosinophilia during rsv challenge. the g protein is remarkable in that it induces cd4+, but no cd8+ t cells in this mouse strain. studies using passive t cell transfers show that co-injection of cd8+ t cells greatly reduces the th2-driven lung eosinophilia caused by g-specific cd4+ t cells. by contrast, vaccination with the fusion protein (f) induces both cd8+ ... | 1997 | 9464822 |
| analysis of the bovine respiratory syncytial virus fusion protein (f) using monoclonal antibodies. | seven monoclonal antibodies (mabs) directed against bovine respiratory syncytial virus (brsv) fusion (f) protein were produced and characterized by radioimmunoprecipitation and immunofluorescence assays. these seven mabs together with the previously described mabs (beeler and van wyke coelingh, 1989) to the f protein of human respiratory syncytial virus (hrsv) were used to study the antigenic variation of 12 strains of ungulate rsv. all except one mabs specific for the hrsv-f protein reacted wit ... | 1997 | 9453129 |
| adrenomedullin gene delivery reduces blood pressure in spontaneously hypertensive rats. | adrenomedullin (adm) is a potent vasoactive peptide. in this study, we explored the effects of a continuous supply of adm by somatic gene delivery on spontaneously hypertensive rats (shr). dna constructs containing the human adm cdna fused to either the cytomegalovirus promoter (cmv-cadm) or rous sarcoma virus 3'-long terminal repeat (rsv-cadm) were intravenously injected into shr through the tail vein. expression of human adm in shr was identified in the kidney, adrenal gland, heart, and lung b ... | 1997 | 9453262 |
| cells and mediators from pharyngeal secretions in infants with acute wheezing episodes. | an acute wheezing episode is the most common feature of severe lower respiratory tract infection during infancy. respiratory syncytial virus (rsv) is the major causative agent. in order to study inflammation during acute wheezing episodes in infants, we wanted to assess the feasibility and contribution of induction of pharyngeal secretions. we therefore compared inflammatory markers in the pharyngeal secretions of 27 infants suffering from acute wheezing episodes with an rsv infection (rsv+) and ... | 1997 | 9426101 |
| adenovirus-mediated gene transfer to cultured nodose sensory neurons. | recent advances have enabled transfer of genes to various types of cells and tissues. the goals of the present study were to transfer genes to nodose sensory neurons using replication-deficient adenovirus vectors and to define the conditions needed to optimize the gene transfer. neurons were dissociated from rat nodose ganglia and maintained in culture. cultures were exposed for 30 min to vectors containing the beta-galactosidase gene lacz driven by either the rous sarcoma virus (rsv) or the cyt ... | 1997 | 9427504 |
| high-level expression of exogenous genes by replication-competent retrovirus vectors with an internal ribosomal entry site. | we report the construction of two types of rous sarcoma virus (rsv)-based replication-competent avian retrovirus vectors, ir1 and ir2 to express an exogenous gene at a very high level. in these vectors, the internal ribosomal entry site (ires) derived from encephalomyocarditis virus (emcv) was inserted between the env gene and an exogenous gene. the ir1 vector retains the splicing acceptor site that is present in the downstream of the env gene while the ir2 vector lacks it. using a v-fos mutant ... | 1997 | 9427541 |
| rsv specimen collection methods: nasal vs. nasopharyngeal. | to compare two types of specimen collection methods for rsv (respiratory syncytial virus): nasal wash and nasopharyngeal wash. | 1997 | 9429523 |
| animal models of respiratory syncytial virus infection. | over the past two decades, animal models of respiratory syncytial virus (rsv) infection have been developed using primates, cotton rats, mice, calves, guinea pigs, ferrets, and hamsters. use of these models has shed light on the mechanisms of vaccine-enhanced disease seen in clinical trials of a formalin-inactivated rsv vaccine and has provided a means for testing efficacy and safety of candidate prophylactic and therapeutic strategies. the development of multiple animal models has coincided wit ... | 1997 | 9431379 |
| human prostate cancer progression models and therapeutic intervention. | our laboratory has developed two cellular models of human prostate cancer progression. the lncap prostate cancer progression model is based upon the well-known cellular interaction between human prostate or bone stromal cells and lncap cells in vivo. the marginally tumorigenic lncap cells acquired tumorigenic and metastatic potential upon cellular interaction with either prostate or bone fibroblasts. a subline termed c4-2 was observed to grow readily in castrated animals and acquired metastatic ... | 1997 | 9436028 |
| [respiratory syncytial viruses: pathogenesis, immunology and disease prevention]. | immunopathologic mechanism has been proposed to be involved in the pathogenesis of respiratory syncytial virus infections. this review examines the current understanding of the role of immunopathologic mechanisms in rsv infections. the role of vaccines in inducing hypersensitivity is also discussed. | 1997 | 9441498 |
| nosocomial respiratory syncytial virus infection in a newborn nursery. | respiratory syncytial virus (rsv) has been recognized as a major nosocomial hazard on pediatric wards, but symptomatic rsv infection is uncommon during the first four weeks of life. we report here four cases of neonatal rsv infection in a special-care newborn nursery and two of them probably acquired the infection nosocomially. by rapid diagnosis using immunofluorescent technique and early implementation of infection control measures, we were able to prevent further spread of rsv infection. | 1997 | 9443085 |
| respiratory syncytial virus infection in infants and young children. | respiratory syncytial virus (rsv) is the most common cause of lower respiratory tract infections in infants and young children. epidemics occur yearly from december to march or april, leading to 100,000 hospitalizations in the united states at an estimated cost of $300 million. physical examination findings may include clear coryza, evidence of respiratory distress, wheezing, and dehydration. complications include apnea. mortality runs as high as 0.5% to 1.5% in hospitalized patients. diagnosis ... | 1997 | 9420581 |
| prevention and treatment recommendations for respiratory syncytial virus infection. background and clinical experience 40 years after discovery. | though 40 years have passed since its discovery, respiratory syncytial virus (rsv), one of the most ubiquitous viruses known, continues to evade most of our efforts to prevent or treat the clinical disease it causes. long recognised as the most common cause of lower respiratory tract infections in virtually all children in the first 2 years of life, it has been increasingly recognised as a cause of more serious disease in several 'high risk' populations. these populations include infants with ca ... | 1997 | 9421694 |
| comparison of sequences of rnas 3 and 4 of rice stripe virus from china with those of japanese isolates. | the sequences were determined of rnas 3 and 4 of a chinese isolate (y) of rice stripe tenuivirus (rstv) and were compared with those of two rstv isolates (m and t) from japan. both rnas of the y isolate were longer than those of the m and t isolates. there was almost complete conservation in the 5' and 3' non-coding regions for each rna between the isolates. the analogous ambisense coding regions for each isolate were exactly the same size and the sequences were highly conserved. the major diffe ... | 1997 | 9421874 |
| respiratory syncytial virus-immunoglobulin intravenous (rsv-igiv) for respiratory syncytial viral infections: part i. | rsv is a highly contagious, devastating disease, especially in high-risk infants. rsv infection typically presents as an upper respiratory tract infection and then may progress to the lower respiratory tract, causing pneumonia and bronchiolitis. the signs, symptoms, and severity of rsv infection vary with age and the number of previous rsv infections. young age, premature birth, a crowded living environment, day care attendance, and exposure to passive smoking are risk factors for more severe rs ... | 1997 | 9423415 |
| adenovirus-mediated gene transfer during initial organogenesis in the mammalian embryo is promoter-dependent and tissue-specific. | replication-defective adenoviruses have received increasing attention as vectors for exogenous gene administration in a variety of experimental and pathological conditions. however, little information exists about their utility for in utero gene therapy, and no information exists concerning their efficacy for gene delivery during initial organogenesis in the mammalian embryo. to evaluate the feasibility of using these vectors for exogenous gene transduction during the initial stages of organogen ... | 1997 | 9425436 |
| analysis of the gene start and gene end signals of human respiratory syncytial virus: quasi-templated initiation at position 1 of the encoded mrna. | the gene start (gs) and gene end (ge) transcription signals of human respiratory syncytial virus (rsv) strain a2 were analyzed in helper-dependent monocistronic and dicistronic minireplicons which were complemented by a standard rsv strain. the gs signal, which is the start site for mrna synthesis, is highly conserved for the first nine genes: 3'-ccccguuua(u/c) (negative sense). this conserved version of the signal was analyzed by "saturation" mutagenesis, in which all 10 positions, as well as o ... | 1997 | 9188557 |
| high level of transgene expression in cell cultures and in the mouse by replication-incompetent adenoviruses harboring modified vai genes. | replication-incompetent adenoviruses are currently used in gene therapy trials. most of the work designed to increase the expression from these vectors concerns the modification of cis sequences of the foreign transcription unit, so as to improve the transcription level or the stability of the mrna. in this report, we show that an alternative strategy based on the coexpression of modified vai genes can efficiently increase gene expression both in cell cultures and in animals. the vai rna is synt ... | 1997 | 9188608 |
| genetic analysis of interactions between gag proteins of rous sarcoma virus. | the yeast two-hybrid system was used to characterize homomeric interactions between the gag proteins of rous sarcoma virus (rsv). the rsv gag precursor was found to interact strongly with itself and not with various control proteins. the rsv gag did not interact significantly with gag proteins of a variety of other retroviruses, including murine leukemia viruses and primate lentiviruses. deletion analysis suggested that two nonoverlapping regions are independently sufficient to mediate rsv gag-g ... | 1997 | 9188638 |
| adeno-associated virus type 2-mediated transfer of ecotropic retrovirus receptor cdna allows ecotropic retroviral transduction of established and primary human cells. | the cellular receptors that mediate binding and internalization of retroviruses have recently been identified. the concentration and accessibility of these receptors are critical determinants in accomplishing successful gene transfer with retrovirus-based vectors. murine retroviruses containing ecotropic glycoproteins do not infect human cells since human cells do not express the receptor that binds the ecotropic glycoproteins. to enable human cells to become permissive for ecotropic retrovirus- ... | 1997 | 9188645 |
| transcriptional induction of multiple cytokines by human respiratory syncytial virus requires activation of nf-kappa b and is inhibited by sodium salicylate and aspirin. | infection of the lung epithelial cell line a549 by respiratory syncytial virus (rsv) resulted in the elevated synthesis of multiple cellular cytokines, including a number of interleukins (ils). detailed studies of il-11 induction revealed that it required infection by viable virus and involved a net increase in the steady state level of il-11 mrna. nuclear run-on assays showed a direct effect of rsv on il-11 gene transcription. mutational analysis of the il-11 promoter fused to a reporter lucife ... | 1997 | 9191851 |
| determination of the disulfide bond arrangement of human respiratory syncytial virus attachment (g) protein by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. | the attachment protein or g protein of the a2 strain of human respiratory syncytial virus (rsv) was digested with trypsin and the resultant peptides separated by reverse-phase high-performance liquid chromatography (hplc). one tryptic peptide produced a mass by matrix-assisted laser desorption/ionization (maldi) time-of-flight (tof) mass spectrometry (ms) corresponding to residues 152-187 with the four cys residues of the ectodomain (residues 173, 176, 182, and 186) in disulfide linkage and abse ... | 1997 | 9194191 |
| il-12 treatment attenuates t helper cell type 2 and b cell responses but does not improve vaccine-enhanced lung illness. | in humans and mice, sensitization to respiratory syncytial virus (rsv) ags can result in severe inflammatory lung disease during subsequent infection with rsv. although specific antiviral t cells are thought to be responsible for this augmentation of disease, the precise roles of different functional subsets are unknown, and no protective nonpathogenic subset has been defined. balb/c mice sensitized to the major surface glycoprotein of rsv (g) expressed by recombinant vaccinia virus develop th2- ... | 1997 | 9200470 |
| respiratory syncytial virus infection results in airway hyperresponsiveness and enhanced airway sensitization to allergen. | viral respiratory infections can predispose to the development of asthma by mechanisms that are presently undetermined. using a murine model of respiratory syncytial virus (rsv) infection, acute infection is associated with airway hyperresponsiveness as well as enhanced responses to subsequent sensitization to allergen. we demonstrate that acute viral infection results in increased airway responsiveness to inhaled methacholine and pulmonary neutrophilic and eosinophilic inflammation. this respon ... | 1997 | 9202075 |
| regulated expression of the diphtheria toxin a gene in human glioma cells using prokaryotic transcriptional control elements. | because accurate regulation of toxin gene expression is critical for safe and effective gene therapy applications, the authors have examined the regulation of diphtheria toxin a (dta) fragment expression in human glioma cell lines using two transcriptional control systems derived from escherichia coli: the tetracycline (tet) system and the lactose (lac) system. the tet system includes a tetracycline-controlled transactivator (tta), a tta-responsive minimum human cytomegalovirus (hcmv) promoter c ... | 1997 | 9202271 |
| construction of recombinant adeno-associated virus vector containing the rat preproinsulin ii gene. | we have investigated a possible delivery system for the rat preproinsulin ii gene (ri2) utilising a recombinant adeno-associated virus (raav) vector system, with the long-term goal of engineering stably infected insulin-producing cell lines. the raav vector was chosen because it is a safe and nonpathogenic method for gene transfer. the plasmid pbc12bi (atcc) was purified and digested with restriction enzymes sepi and stui to release a fragment containing the rous sarcoma virus long terminal repe ... | 1997 | 9202669 |
| prevalence of antibodies against respiratory viruses (parainfluenza virus type 3, respiratory syncytial virus, reovirus and adenovirus) in relation to productivity in syrian awassi sheep. | awassi sheep sera from all the provinces of the syrian arab republic were tested for rsv, p13, reo and adeno viruses (iif and agid tests). rsv was the most prevalent infection with 63.6% of samples seropositive, followed by reo, p13 and adenovirus with seroprevalences of 27.3%, 24% and 8.1% respectively. animals were more frequently infected by rsv alone. mixed infections were also identified but the occurrence was not high. the rsv and reo virus infections occurred more frequently when transhum ... | 1997 | 9203308 |
| baculovirus expression of the respiratory syncytial virus fusion protein using trichoplusia ni insect cells. | respiratory syncytial virus (rsv) is a major viral pathogen responsible for severe respiratory tract infections in infants, young children, and the elderly. the rsv fusion (f) protein is highly conserved among rsv subgroups a and b and is the major protective immunogen. a genetically-engineered version of the rsv f protein was produced in insect cells using the baculovirus expression system. to express a secreted form of this protein, the transmembrane domain was eliminated by removing the regio ... | 1997 | 9208456 |
| adenoviral-mediated herpes simplex virus thymidine kinase gene transfer: regression of hepatic metastasis of pancreatic tumors. | pancreatic cancer is the fifth leading cause of cancer death in the united states. most patients have obvious metastases or locally advanced disease at the time of presentation. surgical resection does not significantly change the clinical outcome. combination chemotherapy induces a partial response but overall survival remains low. the aim of this study was to evaluate the feasibility of adenovirus-mediated suicide gene transduction as a therapeutic approach for pancreatic cancer. a cell line w ... | 1997 | 9211489 |
| adenovirus and respiratory syncytial virus in chronic sinusitis using polymerase chain reaction. | the aim of this study is to investigate the role of adenovirus and respiratory syncytial virus (rsv) in chronic sinusitis using the polymerase chain reaction (pcr) to assay for the viruses. pcr has proved to be more sensitive and specific than viral cultures and immunoassays in the detection of viruses. adenovirus and rsv are among the most common viruses to cause upper respiratory tract infections. sinus mucosa biopsies from 20 patients undergoing endoscopic sinus surgery were sterilely collect ... | 1997 | 9217132 |
| replication-competent retroviral vectors for expressing genes in avian cells in vitro and in vivo. | replication-competent retroviral vectors based on rous sarcoma virus (rsv) are becoming increasingly popular for expressing genes in both primary cell cultures and embryonic chick tissues in ovo. in this article, we review the features of rsv and its life cycle that make it suitable for use as a vector. we describe the design and use of the rcas and rcas (bp) series of vectors, which are currently the most widely used rsv-based vectors, illustrating both their strengths and weakness. finally, we ... | 1997 | 9219242 |
| the temperature-sensitive (ts) phenotype of a cold-passaged (cp) live attenuated respiratory syncytial virus vaccine candidate, designated cpts530, results from a single amino acid substitution in the l protein. | cpts530, a candidate live-virus vaccine, is an attenuated strain of human respiratory syncytial virus (rsv). it was derived by subjecting a cold-passaged (cp) strain of rsv to a single round of chemical mutagenesis. cpts530 is a temperature-sensitive (ts) mutant that is attenuated in mice and chimpanzees, and its ts phenotype exhibits a high level of stability during replication in both species. in the present study, the complete nucleotide sequence of cpts530 rsv was determined. the five mutati ... | 1997 | 9223470 |
| respiratory syncytial virus: not just for kids. | respiratory syncytial virus (rsv) earned its reputation as a pediatric pathogen, especially in children under two years of age. rsv infection in adults was considered a significant problem only for certain high-risk populations. evidence now indicates that the infection occurs frequently in previously healthy adults. clinical presentation varies greatly. | 1997 | 9227660 |
| heparin-dependent attachment of respiratory syncytial virus (rsv) to host cells. | in this study we could demonstrate that heparin (ed50 = 0.32 +/- 0.12 microgram/ml), but not heparan sulphate or chondroitin sulphate c is able to inhibit in vitro infection of cells by respiratory syncytial virus (rsv). in addition, this protective effect of heparin could only be observed, when heparin was present at the time of inoculation. enzymatic digestion of cell surface glycosaminoglycans with heparinase and heparitinase, but not chondroitin sulphate abc lyase reduced the effectiveness o ... | 1997 | 9229012 |
| respiratory syncytial virus infection in infants is associated with predominant th-2-like response. | viral infections have been associated with cellular immune responses and production of th-1 cytokines. respiratory syncytial virus (rsv), however, induces virus-specific ige, which might be a consequence of a th-2-like activation. to test this hypothesis we quantified interferon-gamma (ifn-gamma) and interleukin-4 (il-4) in the supernatant of peripheral blood mononuclear cells cultured for 24 and 48 h in the presence or absence of phytohemaglutinin and pokeweed mitogen and the lymphocyte phenoty ... | 1997 | 9230746 |
| peptide mimics of a conformationally constrained protective epitopes of respiratory syncytial virus fusion protein. | to identify peptides that mimic (mimotopesi conformational and protective epitopes of rsv fusion protein and to assess their efficacy as immunogens and potential vaccines. | 1997 | 9232419 |
| transcriptional and post-transcriptional regulation of kappab-controlled genes by pp60v-src. | the cef-4/9e3 gene is expressed aberrantly in chicken embryo fibroblasts transformed by the rous sarcoma virus. this aberrant expression is dependent on transcriptional activation and on the stabilization of the cef-4 mrna. the characterization of the cef-4 promoter indicated that three distinct regulatory elements corresponding to an ap-1 binding site, a prdii/ kappab domain and a caat box are involved in the activation by pp60v-src. several v-src responsive genes are controlled by ap-1 and mem ... | 1997 | 9233775 |
| protective immune responses induced by the immunization of mice with a recombinant bacteriophage displaying an epitope of the human respiratory syncytial virus. | we investigated whether a recombinant bacteriophage displaying a disease-specific protective epitope could be experimentally used as a vaccine to confer protection of immunized animals against infection. we genetically engineered a recombinant phage, fd, displaying at its surface a chimeric piii coat protein fused to the previously identified protective epitope 173-187 from the glycoprotein g of the human respiratory syncytial virus (rsv). a selected recombinant fd phage elicited a strong immune ... | 1997 | 9234952 |
| virus-specific cd8+ t lymphocytes downregulate t helper cell type 2 cytokine secretion and pulmonary eosinophilia during experimental murine respiratory syncytial virus infection. | t lymphocytes play a pivotal role in the immune response during viral infections. in a murine model of experimental respiratory syncytial virus (rsv) infection, mice sensitized to either of the two major glycoproteins of rsv develop distinct patterns of cytokine secretion and lung inflammation upon subsequent rsv infection. mice sensitized to rsv-g (attachment) glycoprotein exhibit a strong interleukin (il)-4 and il-5 response and develop pulmonary eosinophilia, whereas mice sensitized to rsv-f ... | 1997 | 9236194 |
| randomised double blind placebo controlled trial of prednisolone in children admitted to hospital with respiratory syncytial virus bronchiolitis. | experimental and clinical evidence suggests that respiratory syncytial virus (rsv) bronchiolitis is an immune mediated disease. corticosteroids might therefore be effective in the treatment of rsv bronchiolitis. | 1997 | 9246136 |
| macrophages and respiratory viruses. | this review highlights some of the interactions between alveolar macrophages and human/swine influenza a viruses, porcine reproductive and respiratory syndrome virus (prrsv), bovine parainfluenza type-3 (pi-3) virus, bovine respiratory syncytial virus (brsv) and bovine viral diarrhoea virus (bvdv). in influenza a infection, macrophage-cytokines are believed to mediate the typical constitutional effects and inflammation in the respiratory tract. the same cytokines possibly play a role in influenz ... | 1997 | 9247041 |
| detection of respiratory syncytial virus, parainfluenzavirus 3, adenovirus and rhinovirus sequences in respiratory tract of infants by polymerase chain reaction and hybridization. | immunofluorescence assay (ifa) of viral antigens in nasal aspirates is largely used for the diagnosis of respiratory syncytial virus (rsv), parainfluenzavirus (piv) type 3 and adenovirus (adv) infections, whilst rhinovirus (rv) are detected by virus isolation technique (vit) only. using the two techniques, ifa and vit, a significant number of specimens remain negative in spite of clinical and epidemiological presumptions of viral infection. | 1997 | 9248656 |
| the effect of promoter strength in adenoviral vectors containing herpes simplex virus thymidine kinase on cancer gene therapy in vitro and in vivo. | the use of adenoviral vectors to deliver the herpes simplex virus thymidine kinase (hsvtk) gene followed by treatment with the prodrug ganciclovir (gcv) has promise for a variety of applications where excess cell proliferation is detrimental such as treatment of tumors and vascular restenosis. optimizing this system is thus an important goal. the purpose of this study was to determine if the induction of higher levels of hsvtk expression would augment the sensitivity to gcv. this was accomplishe ... | 1997 | 9253506 |
| targeted transduction of cns neurons with adenoviral vectors carrying neurotrophic factor genes confers neuroprotection that exceeds the transduced population. | application of neurotrophic factors (nfs) to the cut stump of motor nerves of neonatal rats confers neuroprotection from trauma-induced neuronal death. to test whether motoneurons are capable of responding to endogenously produced nfs, facial motoneurons were genetically modified in vivo to express several nfs and then tested for their response to peripheral nerve damage. replication-defective adenoviral vectors [adv. rous sarcoma virus (rsv)-nf] representing three families of nfs were construct ... | 1997 | 9254662 |
| economic evaluation of respiratory syncytial virus infection in canadian children: a pediatric investigators collaborative network on infections in canada (picnic) study. | to quantify the cost and distribution of health care resources consumed annually in management of canadian children from birth to 4 years of age with respiratory syncytial virus (rsv) infection. | 1997 | 9255201 |
| association between pronounced iga response in rsv bronchiolitis and development of allergic sensitization. | forty-five children who had been hospitalized with bronchiolitis caused by respiratory syncytial virus (rsv) at a mean age of 4 months, and 90 matched control children, were tested for occurrence of rsv antibodies at one year of age. of the children who had suffered from bronchiolitis, forty had demonstrable igg antibodies, whereas the remaining five only had iga antibodies against rsv. in the control group, 42% were rsv seropositive. the anti-rsv iga antibody titres tended to be higher in patie ... | 1997 | 9260211 |
| equine infectious anemia virus utilizes a yxxl motif within the late assembly domain of the gag p9 protein. | we have previously demonstrated that the gag p9 protein of equine infectious anemia virus (eiav) is functionally homologous with rous sarcoma virus (rsv) p2b and human immunodeficiency virus type 1 (hiv-1) p6 in providing a critical late assembly function in rsv gag-mediated budding from transfected cos-1 cells (l. j. parent et al., j. virol. 69:5455-5460, 1995). in light of the absence of amino acid sequence homology between eiav p9 and the functional homologs of rsv and hiv-1, we have now desi ... | 1997 | 9261374 |
| priming with live respiratory syncytial virus (rsv) prevents the enhanced pulmonary inflammatory response seen after rsv challenge in balb/c mice immunized with formalin-inactivated rsv. | to investigate enhanced disease associated with a formalin-inactivated (fi) respiratory syncytial virus (rsv) vaccine, we studied the pulmonary inflammatory response to rsv in balb/c mice immunized with live rsv, fi-rsv, or combinations of the two. after rsv challenge, the number of granular cells, the ratio of cd4+/cd8+ lymphocytes, and the level of th2-like cytokine mrnas in the bronchoalveolar lavage specimens in mice immunized first with live rsv and then with fi-rsv were lower than that in ... | 1997 | 9261421 |
| surfactant dysfunction develops in balb/c mice infected with respiratory syncytial virus. | recent reports suggest an important role for pulmonary surfactant in maintaining the patency of narrow conducting airways. the hypothesis that surfactant dysfunction is an important factor in respiratory syncytial virus (rsv) infection was tested in a mouse model. mice, inoculated with either a low or a high dose of rsv, were subjected to bronchoalveolar lavage (bal), and the fluids were analyzed for percentage of inflammatory cells and concentrations of proteins and phospholipids. after concent ... | 1997 | 9262218 |
| immortalized cell lines from embryonic avian and murine otocysts: tools for molecular studies of the developing inner ear. | recently, our studies have focused on genes expressed at the earliest stages of inner ear development. our aim is to identify and characterize genes that are involved in determining the axes of the semicircular canals, in otic crest delamination and in early innervation of the inner ear. many elegant studies of auditory development have been done in animal models. however, the need for large amounts of well-characterized embryonic material for molecular studies makes the development of otocyst c ... | 1997 | 9263030 |
| analysis of bovine respiratory syncytial virus envelope glycoproteins in cell fusion. | to compare the requirements for respiratory syncytial virus (rsv)-mediated cell fusion, the fusion (f), attachment (g) and small hydrophobic (sh) glycoproteins of bovine rsv (brsv), ovine rsv (orsv) and human rsv (hrsv) were expressed individually or coexpressed in either homologous or heterologous combinations in hela cells, using the vaccinia virus-t7 polymerase transient expression system. cell fusion was examined by a reporter gene activation assay. although the expression of the f protein a ... | 1997 | 9266983 |
| safety and immunogenicity of a respiratory syncytial virus subunit vaccine (pfp-2) in the institutionalized elderly. | the safety and immunogenicity of purified fusion protein (pfp-2) respiratory syncytial virus (rsv) vaccine was evaluated in an open label study in 37 frail institutionalized persons over age 65. vaccination was well tolerated without significant side-effects. thirty-six of 37 volunteers completed the study. nineteen of 36 (53%) vaccinees had a greater than or equal to fourfold increase in igg to f protein at 4 weeks and 17 (47%) had a greater than or equal to fourfold rise in neutralizing titers ... | 1997 | 9269057 |
| prevalence of respiratory viruses and mycoplasma pneumoniae in sputum samples from unselected adult patients. | sputum samples from adult patients are routinely used for bacteriological tests, but not for the diagnosis of viral/mycoplasmal infections. we examined 511 sputum samples submitted for bacterial tests from patients at the royal adelaide hospital. each specimen was tested directly (and after six days of cell culture amplification) for antigens to influenza a and b, parainfluenza 1, 2 and 3, adenovirus, respiratory syncytial virus (rsv) and mycoplasma pneumoniae. respiratory viruses or m. pneumoni ... | 1997 | 9271022 |
| inflammatory cells and activation markers in bal during acute rejection and infection in lung transplant recipients: a prospective, longitudinal study. | acute rejection of the transplanted lung is a clinical problem, since it decreases graft survival and predisposes the patient to chronic rejection and obliterative bronchiolitis (ob). in an earlier study, we had indications that eosinophil cationic protein (ecp) from activated eosinophils and hyaluronan (hya) from fibroblasts were associated with acute pulmonary rejection. this prospective longitudinal study was designed to investigate whether molecules from activated inflammatory cells in bronc ... | 1997 | 9272913 |
| a monoclonal antibody pool for routine immunohistochemical detection of human respiratory syncytial virus antigens in formalin-fixed, paraffin-embedded tissue. | four monoclonal antibodies (mabs) with specificities for epitopes on human respiratory syncytial virus (rsv) proteins preserved after formalin fixation and paraffin embedding were identified in fixed and embedded virus-infected hep-2 cell pellets. the mabs bound epitopes on the fusion protein, the nucleoprotein, the phosphoprotein, and the m2 protein of the virus. following high-temperature antigen unmasking, immunohistochemical staining revealed rsv antigens in the lungs of five of seven childr ... | 1997 | 9274537 |
| interrelationship between tumour virus receptors, a and c, coded by host cell genes in fowl. | bryan standard strain of rous sarcoma virus (bs-rsv) of subgroup a and pseudotype of bryan high titre rsv(rav-49) of subgroup c and an equal mixture of subgroup a and subgroup c virus were inoculated to 11-day old embryos of white leghorn (wl), australorp (al) and f1 and f2 generations of crosses between wl and al breeds of fowl to detect and estimate the interrelationship between tumour virus receptor coding host cell genes of tva and tvc loci. linkage values estimated on a pooled sex basis wer ... | 1997 | 9279130 |
| ex vivo adenovirus-mediated gene transfer and immunomodulatory protein production in human cornea. | one attractive strategy to prevent or control allograft rejection is to genetically modify the donor tissue before transplantation. in this study, we have examined the feasibility of gene transfer to human corneal endothelium, using a number of recombinant adenovirus constructs. ex vivo infection of human corneas with adenoviral vectors containing lacz, under transcriptional control of either cytomegalovirus (cmv) or rous sarcoma virus (rsv) promoters, provided high-level gene expression, which ... | 1997 | 9282165 |
| challenge of balb/c mice with respiratory syncytial virus does not enhance the th2 pathway induced after immunization with a recombinant g fusion protein, bbg2na, in aluminum hydroxide. | the polypeptide of aa 130-230 of the g protein (g2na) of respiratory syncytial virus (rsv) was fused to bb, the albumin-binding region of streptococcal g protein, producing bbg2na, which induced protective immune responses in rodent models. evaluation of the immune response in mice immunized with bbg2na in the adjuvant alhydrogel revealed high amounts of interleukin (il)-5 and some il-4 in splenocytes restimulated in vitro. this is compatible with a th2 response. the activation of the th2 pathwa ... | 1997 | 9291300 |
| increased expression of the n protein of respiratory syncytial virus stimulates minigenome replication but does not alter the balance between the synthesis of mrna and antigenome. | a popular model for rna synthesis by nonsegmented negative-strand rna viruses is that transcription and rna replication are executed by the same polymerase complex and that there is a dynamic balance between the two processes that is mediated by the nucleocapsid n protein. according to this model, transcription occurs until sufficient soluble n protein accumulates to initiate encapsidation of the nascent rna product, which somehow switches the polymerase into a readthrough replicative mode. this ... | 1997 | 9299631 |
| molecular characterization of an outbreak of respiratory syncytial virus (subgroup a) in havana, cuba, by monoclonal antibodies and restriction mapping (n gene). | twenty-one respiratory syncytial virus (rsv) strains isolated from one outbreak in havana, cuba (1994 to 1995), were analyzed to determine their relatedness. all isolated strains were classified as subgroup a by monoclonal antibodies. of 21 rsv strains examined, 20 were classified as having restriction pattern np4 and only 1 was classified as having restriction pattern np5. | 1997 | 9302221 |
| immunization with a peptide derived from the g glycoprotein of bovine respiratory syncytial virus (brsv) reduces the incidence of brsv-associated pneumonia in the natural host. | previous reports demonstrate that synthetic peptides corresponding to the amino acid region 174-187 of g glycoprotein from subgroups a and b human respiratory syncytial virus (hrsv), containing a cys-->ser substitution at position 186, confer complete resistance to immunized balb/c mice against infection with the respective virus. in this report, we show that a cys186-->ser substituted peptide (bg/174-187) representing the corresponding region of the bovine (b) rsv g glycoprotein conferred compl ... | 1997 | 9302749 |
| effect of rapid viral diagnosis on the management of children hospitalized with lower respiratory tract infection. | although rapid viral tests are commonly used in children with lower respiratory tract infection, their effect on patient management has not been studied. | 1997 | 9306477 |
| usefulness of a mouse myelin basic protein promoter for gene therapy of malignant glioma: myelin basic protein promoter is strongly active in human malignant glioma cells. | we have searched for suitable promoters to regulate the expression of suicide genes for use in gene therapy. we have shown that the 1.3-kb fragment of the mouse myelin basic protein (mbp) promoter region initiates transcription in mouse glioma cells more efficiently than glial fibrillary acidic protein (gfap) or myelin proteolipid protein (plp) promoter. among three different lengths of the mbp promoter, the shortest (256-bp) core promoter region initiates transcription as efficiently as 650-bp ... | 1997 | 9310141 |
| multiple biological roles associated with the rous sarcoma virus 5' untranslated rna u5-ir stem and loop. | the 5' untranslated region of rous sarcoma virus (rsv) rna is a highly ordered structure involved in multiple processes in the viral replication cycle. one of these structures, referred to as the u5-ir stem, is located immediately upstream of the 5' end of the primer binding site. disruption of its base pairing results in a decrease in initiation of reverse transcription (d. cobrinik, a. aiyar, z. ge, m. katzman, h. huang, and j. leis, j. virol. 65:3864-3872, 1991). in the present study, the len ... | 1997 | 9311847 |
| selection of an avian retrovirus mutant with extended receptor usage. | receptor recognition by avian retroviruses is thought to involve the interaction of two regions of the su protein, hr1 and hr2, with the host cell surface receptor. these regions exhibit considerable variation, concordant with differences in receptor usage among the many avian leukosis virus subgroups. we hypothesize that some retroviruses have altered receptor usage in response to selective pressures imposed by receptor polymorphisms in their hosts. to test this hypothesis, we passaged td-pr-rs ... | 1997 | 9311868 |
| the incidence of respiratory tract infection in adults requiring hospitalization for asthma. | acute respiratory tract infections (rti) are known to worsen asthma particularly in children. there are few studies in adults assessing the incidence of rti in patients hospitalized with acute asthma. | 1997 | 9315789 |
| rod-shaped virus-like particles in intestinal contents of pheasant chicks. | rod-shaped virus-like particles (rsv), 55-85 nm in length and 18 nm in diameter, with 5 to 10 segments or helical turns, were demonstrated in the intestinal contents of young diarrhoeic pheasants by examination of a fresh sample. the origin of rsv seems to be splitting tails of bacteriophages. | 1997 | 9323932 |
| analysis of respiratory syncytial virus in clinical samples by reverse transcriptase-polymerase chain reaction restriction mapping. | the aim of this study was to develop a polymerase chain reaction (pcr) for the detection of respiratory syncytial virus (rsv) genomes. the primers were designed from published sequences and selected from conserved regions of the genome encoding for the n protein of subgroups a and b of rsv. pcr was applied to 20 specimens from children admitted to the respiratory ward of "william soler" pediatric hospital in havana city with a clinical diagnosis of bronchiolitis. the pcr was compared with viral ... | 1997 | 9332606 |
| protective immunity against respiratory syncytial virus in early life after murine maternal or neonatal vaccination with the recombinant g fusion protein bbg2na. | maternal and neonatal immunization were evaluated for their capacity to induce protective immunity against respiratory syncytial virus (rsv) lower respiratory tract infections in early life. murine models were studied by use of a novel recombinant vaccine candidate, designated bbg2na, which was derived in part from the rsv (long) g protein. maternal immunization resulted in the passive transfer of high levels of rsv-a antibodies to the offspring, which protected them from rsv challenge for up to ... | 1997 | 9333145 |
| contribution of tumor necrosis factor alpha and interleukin-1 alpha on the production of macrophage inflammatory protein-2 in response to respiratory syncytial virus infection in a murine macrophage cell line, raw264.7. | the production of several inflammatory cytokines, such as murine macrophage inflammatory protein-2 (mip-2), tumor necrosis factor (tnf), and interleukin (il)-1, was investigated in response to respiratory syncytial virus (rsv) infection in a murine macrophage cell line, raw264.7, with special reference to mutual relation of their productions. the kinetics of mip-2 production showed a trend for a biphasic pattern, that is, mip-2 levels became detectable from 2 h postinfection (p.i.) and increased ... | 1997 | 9334925 |
| home-based immunoglobulin therapy reduces incidence of neonatal rsv infection. | respiratory syncytial virus (rsv) infections are responsible for more than 90,000 hospitalizations and 4500 infant and childhood deaths each year. rsv infection, which peaks between january and march, can cause respiratory problems, such as bronchiolitis and pneumonia, in children younger than 2 years old. | 1997 | 9335686 |
| adenovirus-mediated gene transfer of a human il-6 antagonist. | il-6 is a pleiotropic cytokine and plays a major role in inflammation and in the immune response. altered serum levels of il-6 have been described in several pathologies such as myeloma, ebv-lymphoma and chronic autoimmune disease. here we report data on the utilization of a hil-6 receptor superantagonist with a gene therapy approach. the superantagonist used in this work possesses very high affinity for the hil-6 receptor, and is therefore an excellent candidate for the treatment of il-6-depend ... | 1997 | 9338013 |
| constitutive expression of murine ctla4ig from a recombinant adenovirus vector results in prolonged transgene expression. | the administration of soluble muctla4ig around the time of adenovirus vector mediated gene transfer into murine hepatocytes has been shown to markedly prolong transgene expression, diminish the formation of adenovirus neutralizing antibody, decrease t cell proliferative response and infiltration into the liver without causing irreversible systemic immunosuppression. in this study, an e1/e3-deleted adenovirus vector constitutively expressing murine ctla4ig (ad.rsv-muctla4ig) was constructed in or ... | 1997 | 9338015 |
| uptake of plasmid rsv dna by frog and mouse spermatozoa. | xenopus laevis spermatozoa and mouse epididymal spermatozoa were compared in their ability to bind plasmid dna. spermatozoa of both species are endowed with a very similar binding capacity for plasmid paprc dna carrying the complete rous sarcoma virus (rsv) dna. our experiments failed to detect any substantial differences between both types of sperm cells in the kinetics of dna binding, maximum dna binding capacity, effect of various ions, metabolic inhibitors and substrates on dna binding, etc. ... | 1997 | 9338119 |
| allergic sensitization increases airway reactivity in guinea pigs with respiratory syncytial virus bronchiolitis. | respiratory syncytial virus (rsv) causes acute bronchiolitis in children and has been implicated in the pathogenesis of recurrent wheezing and asthma. however, few children exposed to rsv experience acute bronchiolitis or its sequelae, suggesting a subgroup of susceptible children. an allergic diathesis may predispose children to subsequent airway disease. | 1997 | 9338543 |
| comparison of various expression plasmids for the induction of immune response by dna immunization. | intramuscular injection of plasmid dna is an efficient method to introduce a foreign gene into a live animal. we investigated several factors affecting the gene transfer efficiency and the following immune response by intramuscular injection of plasmid dna. when the strength of several highly efficient viral promoters was compared in muscle by using the chloramphenicol acetyltransferase (cat) gene as an indicator, cytomegalovirus (cmv) immediate early promoter was found to be stronger than any o ... | 1997 | 9339893 |
| current status of respiratory syncytial virus (rsv) and parainfluenza virus type 3 (piv3) vaccine development: memorandum from a joint who/niaid meeting. | a joint who/national institute of allergy and infectious diseases (niaid) meeting on the current status of respiratory syncytial virus (rsv) and parainfluenza virus type 3 (piv3) vaccine development was held in bethesda, md, from 30 september to 1 october 1996. the meeting summarized the worldwide impact of rsv and piv3; presented the current status of development of rsv and piv3 vaccines; and examined the applications of recombinant dna technology to the development and characterization of vacc ... | 1997 | 9342889 |
| overexpression of interleukin-4 delays virus clearance in mice infected with respiratory syncytial virus. | although interleukin-4 (il-4) expression has been implicated in vaccine-enhanced respiratory syncytial virus (rsv) disease, its role in mediating the immune response to primary rsv infection remains unclear. to assess the effect of il-4 production on typical rsv infection, transgenic mice which either overexpress or fail to express il-4 were challenged intranasally with rsv and their responses were compared to those of the parent strains. il-4-deficient mice eliminated virus from the lung as qui ... | 1997 | 9343225 |
| simplified, rapid diagnosis of respiratory syncytial virus from clinical specimens. | dot elisa was compared with rt-pcr and tissue culture to detect rsv from nasopharyngeal aspirates. dot elisa had diagnostic sensitivity and specificity of 65.62% and 93.92%, respectively. the results indicate that dot elisa can be used for screening detection of rsv from clinical specimens and is suitable for small laboratories in the provincial areas of developing countries. | 1997 | 9346274 |
| systemic and local immune responses of four cases with lower respiratory tract illness due to reinfection with respiratory syncytial virus. | four infants and children who had tracheobronchitis or bronchiolitis by respiratory syncytial virus (rsv) reinfection were presented. their clinical features, systemic and local immune reactions to rsv were compared with those of eleven age-matched patients who had lower respiratory tract illness with primary rsv infection. moderate antibody activities in their nasopharyngeal secretions and sera which were comparable to those of convalescent phase of primary infection were observed within severa ... | 1997 | 9354358 |
| development of a humanized monoclonal antibody (medi-493) with potent in vitro and in vivo activity against respiratory syncytial virus. | neutralizing polyclonal antibody to respiratory syncytial virus (rsv) has been shown to be an effective prophylactic agent when administered intravenously in high-risk infants. this study describes the generation of a humanized monoclonal antibody, medi-493, that recognizes a conserved neutralizing epitope on the f glycoprotein of rsv. the affinity of medi-493 was found to be equal to or slightly better than an isotype-matched chimeric derivative of the parent antibody. in plaque reduction, micr ... | 1997 | 9359721 |
| influence of maternal infections with viral agents or toxoplasma gondii during pregnancy on fetal ige production. | the importance of maternal infections with toxoplasma gondii, cytomegalovirus (cmv), parvovirus b19, respiratory syncytial virus (rsv), and influenza a and b on fetal ige synthesis was studied in 153 pregnant women. no case of specific igm activity or viral dna in cord blood, indicating a congenital infection, was found. from gestational week 15 to delivery, maternal igg-ab seroconversion to parvovirus b19, rsv, influenza a, or influenza b occurred in 47 women. at delivery, serologic signs of pa ... | 1997 | 9360748 |
| efficacy of bronchodilators in the treatment of bronchiolitis. | bronchiolitis is a common respiratory infection affecting young children. much controversy revolves around the efficacy of bronchodilators in the treatment of bronchiolitis. this study was conducted to address this issue. | 1997 | 9364884 |
| recombinant respiratory syncytial virus from which the entire sh gene has been deleted grows efficiently in cell culture and exhibits site-specific attenuation in the respiratory tract of the mouse. | the small hydrophobic protein sh of human respiratory syncytial virus (rsv) is a short transmembrane surface protein of unknown function. a full-length cdna of rsv strain a2 (subgroup a) antigenomic rna was modified such that the entire sh gene, including the transcription signals and the complete mrna-encoding sequence, was deleted and replaced by a synthetic intergenic region. this reduced the length of the antigenome by 398 nucleotides and ablated expression of 1 of the 10 rsv mrnas. recombin ... | 1997 | 9371553 |
| rous sarcoma virus direct repeat cis elements exert effects at several points in the virus life cycle. | two approximately 135-nucleotide (nt) direct repeats flank the rous sarcoma virus (rsv) oncogene src and are composed of two smaller repeats, dr1 (approximately 100 nt) and dr2 (approximately 36 nt). these sequences have been reported to contain cis-acting signals necessary for rna packaging and elements that allow cytoplasmic accumulation of unspliced rna (cytoplasmic transport elements). in this report, we show that avian fibroblasts infected with the prague a strain of rsv with precise deleti ... | 1997 | 9371572 |
| respiratory syncytial virus immune globulin treatment of rsv lower respiratory tract infection in previously healthy children. | to evaluate the efficacy of high titer respiratory syncytial virus (rsv) immune globulin (rsvig) in the treatment of previously healthy children hospitalized with proven rsv lower tract infection (lri). | 1997 | 9374560 |
| nosocomial respiratory syncytial virus infection in canadian pediatric hospitals: a pediatric investigators collaborative network on infections in canada study. | to determine nosocomial transmission of respiratory syncytial virus (rsv) in canadian pediatric hospitals, outcomes associated with nosocomial disease, and infection control practices. | 1997 | 9374561 |
| crystals of rous sarcoma virus capsid protein show a helical arrangement of protein subunits. | crystals of rous sarcoma virus (rsv) capsid protein diffract x rays to 3.5 a resolution and belong to the monoclinic space group c2 with unit cell parameters a = 374.4 a, b = 128.1 a, c = 200.2 a, and beta = 121.8 degrees. one asymmetric unit of the crystal may contain between 28 and 35 molecules, based on reasonable crystal density assumptions. a self-rotation function and patterson synthesis suggest that rsv capsid protein crystallizes as a helical array. the determinants of the viral particle ... | 1997 | 9375011 |
| rehospitalization with respiratory syncytial virus after neonatal intensive care unit discharge: a 3-year follow-up. | this study sought to determine 1) the incidence of rehospitalization with respiratory syncytial virus (rsv) infection within a 3-year follow-up among infants discharged from the neonatal intensive care unit (nicu), and 2) to examine associations between age at readmission and medical and sociodemographic characteristics among infants rehospitalized with rsv. | 1997 | 9382909 |
| long term follow-up of children hospitalized with respiratory syncytial virus lower respiratory tract infection and randomly treated with ribavirin or placebo. | to determine the long term effects of ribavirin therapy in children hospitalized for respiratory syncytial virus (rsv) lower respiratory tract infection. | 1997 | 9384333 |
| molecular mechanics calculations on rous sarcoma virus protease with peptide substrates. | molecular models of rous sarcoma virus (rsv) protease and 20 peptide substrates with single amino acid substitutions at positions from p4 to p3', where the scissile bond is between p1 and p1'. were built and compared with kinetic measurements. the unsubstituted peptide substrate. pro-ala-val-ser-leu-ala-met-thr, represents the nc-pr cleavage site of rsv protease. models were built of two intermediates in the catalytic reaction, rsv protease with peptide substrate and with the tetrahedral interme ... | 1997 | 9385639 |
| respiratory syncytial virus (rsv) sh and g proteins are not essential for viral replication in vitro: clinical evaluation and molecular characterization of a cold-passaged, attenuated rsv subgroup b mutant. | a live, cold-passaged (cp) candidate vaccine virus, designated respiratory syncytial virus (rsv) b1 cp-52/2b5 (cp-52), replicated efficiently in vero cells, but was found to be overattenuated for rsv-seronegative infants and children. sequence analysis of reverse-transcription-pcr-amplified fragments of this mutant revealed a large deletion spanning most of the coding sequences for the small hydrophobic (sh) and attachment (g) proteins. northern blot analysis of cp-52 detected multiple unique re ... | 1997 | 9391135 |
| the need for inotropic support in a subgroup of infants with severe life-threatening respiratory syncytial viral infection. | we experienced an unusual complication of life-threatening respiratory syncytial viral disease cardiovascular compromise. life-threatening respiratory syncytial virus (rsv) infection has predominancy involved with ventilatory support for respiratory distress and/or failure. we performed a retrospective chart review of 20 consecutive infants admitted to the pediatric intensive care unit (picu) for impending respiratory failure. | 1997 | 9394100 |
| evaluation of two live, cold-passaged, temperature-sensitive respiratory syncytial virus vaccines in chimpanzees and in human adults, infants, and children. | two live-attenuated, cold-passaged (cp), temperature-sensitive (ts) candidate vaccines, designated cpts530/1009 and cpts248/955, were attenuated, genetically stable, and immunogenic in chimpanzees and were highly attenuated for human adults. in respiratory syncytial virus (rsv)-seropositive children, cpts530/1009 was more restricted in replication than cpts248/955. in seronegative children, 10(4) pfu of cpts248/955 was insufficiently attenuated, and a high titer of vaccine virus was shed (mean p ... | 1997 | 9395351 |
| rate of seasonal spread of respiratory syncytial virus in a pediatric hospital. | the rate of nosocomial respiratory syncytial virus (rsv) infection was measured in a large pediatric hospital using an incidence density method. the at-risk days for nosocomial rsv were summed during a defined winter period in which there were 54 admissions with community-acquired rsv infection giving a rate of 2.9 cases per 1,000 at-risk days (95% confidence interval, 0.3-5.4 per 1,000). | 1997 | 9397377 |