Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| vitellogenin in rare minnow (gobiocypris rarus): identification and induction by waterborne diethylstilbestrol. | rare minnow (gobiocypris rarus) is a tiny chinese carp that has a short life cycle and is easily cultured in the laboratory. in this study, juvenile rare minnows were exposed to waterborne diethylstilbestrol (des) at 0.05, 0.5 and 5 microg/l in laboratory aquaria. after exposure for 4, 8, 13 and 21 days, juvenile fish were collected and vitellogenin (vtg) was measured in whole body homogenates. native and sds electrophoresis followed by western blotting were performed for vtg identification, and ... | 2004 | 15171953 |
| structural characterisation of a cyprinid (cyprinus carpio l.) crh, crh-bp and crh-r1, and the role of these proteins in the acute stress response. | we elucidated the structure of the principle factors regulating the initiation of the acute stress response in common carp: corticotrophin-releasing hormone (crh), crh-receptor 1 (crh-r1) and crh-binding protein (crh-bp). phylogenetic analyses reveal that these proteins are evolutionarily well conserved in vertebrates. crh and crh-bp expression are not co-localised in the same hypothalamic perikarya. on the contrary, crh-bp expression is limited to the perimeter of the nucleus preopticus (npo), ... | 2004 | 15171705 |
| presence of multiple isoforms of tnf alpha in carp (cyprinus carpio l.): genomic and expression analysis. | 2004 | 15145420 | |
| some polybrominated diphenyl ether (pbde) flame retardants with wide environmental distribution inhibit tcdd-induced erod activity in primary cultured carp (cyprinus carpio) hepatocytes. | ethoxyresorufin-o-deethylase (erod) activity, a catalytic function of the cytochrome p450 1a (cyp1a) microsomal oxygenase subfamily, is a popular biomarker for exposure to xenobiotics, polyhalogenated aromatic hydrocarbons (phahs) in particular. it has found wide use in aquatic pollution assessment both in vivo and in vitro. in such studies, subjects are often exposed to complex mixtures where various constituents can interfere with erod-activity, possibly resulting in inadequate estimation of t ... | 2004 | 15145223 |
| designing of novel carbonic anhydrase inhibitors and activators. | carbonic anhydrases (cas, ec 4.2.1.1) are wide spread enzymes, present in mammals in at least 14 different isoforms: some of these isozymes are cytosolic (ca i, ca ii, ca iii, ca vii), while others are membrane-bound (ca iv, ca ix, ca xii and ca xiv); ca v is mitochondrial, and ca vi is secreted in the saliva. three acatalytic forms are also known (carp viii, carp x and carp xi). several important physiological and physio-pathological functions are played by many ca isozymes, which are strongly ... | 2004 | 15328829 |
| stabilisation of scoliosis in two koi (cyprinus carpio). | two koi (cyprinus carpio) from the same pond developed similar lesions of scoliosis. radiographic examinations showed that their spines had become malaligned as a result of vertebral compression fractures involving t14 to t16. the vertebrae in both fish were stabilised with screws, k-wire and polymethylmethacrylate. they both appeared to improve after surgery, but they began to decline and died within three months. a postmortem examination revealed multi-organ inflammation that was not associate ... | 2004 | 15328741 |
| designing of novel carbonic anhydrase inhibitors and activators. | carbonic anhydrases (cas, ec 4.2.1.1) are wide spread enzymes, present in mammals in at least 14 different isoforms: some of these isozymes are cytosolic (ca i, ca ii, ca iii, ca vii), while others are membrane-bound (ca iv, ca ix, ca xii and ca xiv); ca v is mitochondrial, and ca vi is secreted in the saliva. three acatalytic forms are also known (carp viii, carp x and carp xi). several important physiological and physio-pathological functions are played by many ca isozymes, which are strongly ... | 2004 | 15320807 |
| visible light affects chemiluminescence of carp (cyprinus carpio) blood leukocytes. | visible light irradiations at doses of 5 and 12 j/cm(2) were applied to carp buffy coat leukocytes. | 2004 | 15315734 |
| in situ and laboratory bioassays to evaluate the impact of effluent discharges on receiving aquatic ecosystems. | effluents are a main source of direct and often continuous input of pollutants into aquatic ecosystems with long-term implications on ecosystem functioning. therefore, the study of the effects of effluent exposure on organisms, populations or communities within the framework of impact assessment has a high ecological relevance. the aim of this study was to assess the toxicological impact of two effluents, one household wastewater treatment effluent (effluent 1) and one industrial effluent (efflu ... | 2004 | 15312937 |
| effect of naproxen on tail fin regeneration in teleost. | in the present investigation we studied the effect of naproxen, a non-steroidal anti-inflammatory drug used for the treatment of rheumatic disease, on the synthesis of the lepidotrichial matrix of the tail fin of the teleost fish cyprinus carpio (carp) during the regeneration process. the lepidotrichial synthesis was observed by standard and polarized light microscopy and by transmission electron microscopy. in general, naproxen at the dose used in the present study did not affect the organizati ... | 2004 | 15311675 |
| trends in two year risk of repeat revascularisation or death from cardiovascular disease after coronary artery bypass grafting or percutaneous coronary intervention in western australia, 1980-2001. | to investigate whether, over the 21 year period 1980-2001, there had been a reduction in the risk of repeat revascularisation or death from cardiovascular disease in the cohort of all patients who were treated by coronary revascularisation in western australia. | 2004 | 15310696 |
| trends in coronary artery revascularisation procedures in western australia, 1980-2001. | to describe trends in the use of coronary artery revascularisation procedures (carps) and to determine whether or when carp rates will stabilise. | 2004 | 15310694 |
| immunohistochemical distribution of neuropeptide y in the mesencephalon and rhombencephalon of carp, cyprinus carpio l. (cyprinidae: teleostei). | the localization of neuropeptide y (npy)-immunoreactive elements was investigated in the mesencephalon and rhombencephalon of carp, cyprinus carpio, by using antisera raised against porcine npy and the immunoperoxidase technique. concurrently, to identify the distribution of npy-immunoreactivity, we developed an atlas of the studied areas based on nissl-stained sections. the npy-immunoreactive (npy-ir) elements were located in many zones of the mesencephalon and rhombencephalon. in the mesenceph ... | 2004 | 15275652 |
| characterization of a new c-type lectin from common carp cyprinus carpio. | a new c-type lectin with putative mannose specificity was identified from the expressed sequence tag (est) analysis of cdna library from common carp head kidney (hk), stimulated with concanavalin a and lipopolysaccharide (lps) during a routine est analysis. the full sequence of 627 bp was identified by 5'-rapid amplification of cdna ends. the gene is composed of 146 amino acid residues, including an 18-residue signal peptide for secretion and a single carbohydrate-recognition domain of approxima ... | 2004 | 15261461 |
| decontamination effect of electrolysed nacl solutions on carp. | to evaluate the efficacy of electrolysed nacl solutions (ew) for disinfecting bacterial isolates from carp, and the potential application of ew to reducing the bacterial load in whole carp and carp fillets. | 2004 | 15242456 |
| modulative influence of lysozyme dimer on defence mechanisms in the carp (cyprinus carpio) and european sheatfish (silurus glanis) after suppression induced by herbicide roundup. | immunomodulation is a commonly used method of prophylaxis in humans and animals. lysozyme dimer (klp-602) was used at a dose of 50 ug/kg b.w. in order to correct the immunosuppression caused by the action of herbicide glyphosate (roundup- monsanto), which was used in a single bath for 10 minutes in a concentration of 100 mg/l of water. the investigations were carried out on 2 species of fish: the carp (cyprinus carpio l.) and european catfish (silurus glanis l.). herbicide glyphosate caused a de ... | 2004 | 15230544 |
| modulation in hepatic and head kidney parameters of carp (cyprinus carpio l.) induced by copper and chitosan. | copper is used in treatment mixtures to control fungal diseases in vineyards plants. high concentrations of copper are inducing antioxidant stress in some aquatic ecosystems, and potential bioaccumulation in aquatic organisms has prompted the demand for alternative use of low toxic molecules in culture treatments. chitosan is a biomolecule with antifungal and heavy metal ion chelating properties that may be used as a biopesticide. in this study, we investigate the potential toxicity of chitosan ... | 2004 | 15228950 |
| localization, expression and control of adrenocorticotropic hormone in the nucleus preopticus and pituitary gland of common carp (cyprinus carpio l.). | adrenocorticotropic hormone (acth) takes a central role in the hypothalamo-pituitary-interrenal axis (hpi axis), which is activated during stress. acth is produced by the corticotrope cells of the pituitary pars distalis (pd) and is under control of factors from the nucleus preopticus (npo). the distribution of acth in the hypothalamo-pituitary system in common carp (cyprinus carpio l.) was assessed by immunohistochemistry. acth and beta-endorphin immunoreactivity was observed in the acth cells ... | 2004 | 15225128 |
| specific cytotoxic activity generated by mixed leucocyte culture in ginbuna crucian carp. | 2004 | 15212738 | |
| in vitro effects of steroid hormones on igm-secreting cells and igm secretion in common carp (cyprinus carpio). | the effects of steroid hormones on in vitro igm-secreting cells (igmsc) and igm secretion by lymphocytes of the lymphoid organs in common carp, cyprinus carpio were examined by elispot and elisa assay, respectively. cells isolated from peripheral blood (pb), spleen and head kidney were cultured for 12, 24 and 48 h either in the absence or in the presence of steroid hormones, i.e. cortisol, testosterone, 11-ketotestosterone (11-kt), and estradiol-17beta (e(2)) at doses of 1, 10 and 100 ng/ml. cor ... | 2004 | 15212735 |
| hydrodynamics of burst swimming fish larvae; a conceptual model approach. | burst swimming of fish larvae is analysed from a hydrodynamic point of view. a picture of the expected flow pattern is presented based on information in literature on unsteady-flow patterns around obstacles in the intermediate reynolds number region. it is shown that the acceleration stage of burst swimming under restricted conditions can be treated as a frictionless impulsive motion. the stream pattern resulting from this motion is presented and the efficiency of locomotion during the accelerat ... | 2004 | 15207478 |
| molecular cloning of proopiomelanocortin cdna and multi-tissue mrna expression in channel catfish. | channel catfish (ictalurus punctatus) proopiomelanocortin (pomc) cdna was cloned to investigate its structure, evolution, and expression in different tissues. pomc is an important gene in the hypothalamus-pituitary-adrenal axis, the main mediator of the stress response. pomc gene was isolated from a pituitary cdna library and nucleotide sequence was determined. pomc cdna is composed of 1164 nucleotides with a 639 nucleotide open reading frame encoding a protein of 212 amino acids. catfish pomc p ... | 2004 | 15201069 |
| effect of cadmium on blood plasma biochemistry in carp (cyprinus carpio l.). | 2004 | 15199987 | |
| effect of cadmium on hematological indices of common carp (cyprinus carpio l.). | 2004 | 15199986 | |
| sequences of transgene insertion sites in transgenic f4 common carp. | 2004 | 15198197 | |
| hematologic reference intervals for koi (cyprinus carpio), including blood cell morphology, cytochemistry, and ultrastructure. | hematologic data are used routinely in the health care of humans and domestic mammals. similar data for fish are largely fragmentary or have not been collected. | 2004 | 15195266 |
| epithelio-reticular cell thymoma in carp, cyprinus carpio l: an ultrastructural study. | 2004 | 15189377 | |
| response of cyprinus carpio (linn) to sublethal concentration of cypermethrin: alterations in protein metabolic profiles. | freshwater fish, cyprinus carpio were exposed to sublethal concentration (1.2 microg l(-1)) of cypermethrin for 6, 12, 24 and 48 h to analyze various parameters of protein metabolism in functionally different tissues. total, structural and soluble proteins showed decrement; where as free amino acids and the activities of protease, aspartate aminotransferase and alanine aminotransferase significantly increased in cypermethrin exposed fish. interestingly, ammonia content decreased but urea and glu ... | 2004 | 15183996 |
| purification and functional assessment of c3a, c4a and c5a of the common carp (cyprinus carpio) complement. | promotion of inflammatory response is an important role of the complement system, but this kind of function is poorly documented for the lower vertebrates. here we report chemotactic activity of purified anaphylactic fragments derived from the complement components c3, c4 and c5 of the common carp. the purified anaphylatoxins are two c5a-desarg peptides derived from the c5-i isotype, an intact form and a desarg form of c4a from c4-2 isotype, and an intact form and a desarg form of c3a from c3-h1 ... | 2004 | 15183031 |
| cadmium, chromium, and copper induce polychromatocyte micronuclei in carp (cyprinus carpio l.). | 2004 | 15058657 | |
| comparative responses of molluscs and fish to environmental estrogens and an estrogenic effluent. | it is now well established that there is a diverse array of chemical discharged into the environment that can mimic or antagonise the action of hormones. these endocrine-disrupting chemicals (edcs) can thus interact with physiological systems and cause alterations in development, growth and reproduction in wildlife that are exposed to them. as yet, however, there is little information on the relative sensitivities of different wild life groups to these chemicals and/or mixtures of them (e.g. est ... | 2004 | 15046010 |
| induction of apoptosis in a carp leucocyte cell line infected with turbot (scophthalmus maximus l.) rhabdovirus. | a rhabdovirus was observed from the diseased turbot (scophthalmus maximus l.) with lethal syndrome. in this study, a carp leucocyte (clc) cell line was used to investigate the infection process and cell death mechanism occurring during the virus infection. strong cytopathogenic effect (cpe) and the morphological changes, such as extreme chromatin condensation, nucleus fragmentation, and apoptotic body formation, were observed under fluorescence microscopy after dapi staining in the infected clc ... | 2004 | 15041179 |
| debromination of polybrominated diphenyl ether congeners bde 99 and bde 183 in the intestinal tract of the common carp (cyprinus carpio). | polybrominated diphenyl ether (pbde) congener patterns in biota are often enriched in tetra-, penta-, and hexabrominated diphenyl ethers, which is believed to result from the use of the commercial "pentabde" formulation. however, our evidence suggests that debromination of pbdes occurs within fish tissues leading to appreciable accumulation of less brominated congeners. this suggests that pbde body burdens can reflect both direct uptake from exposure and debromination of more highly brominated c ... | 2004 | 14998018 |
| ampa receptor is involved in transmission of cone signal to on bipolar cells in carp retina. | the present work focuses on characterization of glutamate receptor subtypes mediating cone signal transmission to on bipolar cells (bcs) in the carp retina, using intracellular recording techniques. glutamate (5 mm) hyperpolarized cone-dominant on bcs, which was associated with a suppression of light responses, whereas co(2+) (1 mm) depolarized these cells and suppressed their light responses. on the other hand, alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (ampa) of 20 microm caused ... | 2004 | 14988037 |
| tissue-specific oxidative stress responses in fish exposed to 2,4-d and azinphosmethyl. | species- and tissue-specific defenses against the possibility of oxidative stress and lipid peroxidation were compared in adult fish, oreochromis niloticus and cyprinus carpio, exposed to 2,4-dichlorophenoxyacetic acid (2,4-d), azinphosmethyl and their combination for 96 h. superoxide dismutase (sod), glutathione peroxidase (gpx) and catalase activities were monitored in kidney, brain and gill. in all exposure groups there was a marked increase in sod activity in gill tissues in both fish specie ... | 2004 | 14984703 |
| induction and binding of cd, cu, and zn to metallothionein in carp (cyprinus carpio) using hplc-icp-tofms. | the binding of cd, cu, and zn to metallothionein in carp was studied under control and acute cd exposure scenarios. carp were exposed to different cd concentrations for 96 h. total (cu, cd, zn)-mt levels were determined by the cadmium thiomolybdate saturation assay. total tissue and cytosolic cd, cu, and zn concentrations were determined by icp-ms. the cytosolic metal speciation was determined by high pressure liquid chromatography (size-exclusion [se] in combination with anion exchange [ae]) di ... | 2004 | 15103052 |
| seasonal environmental changes modulate the prolactin receptor expression in an eurythermal fish. | eurythermal fish have evolved compensatory responses to the cyclical seasonal changes of the environment. the complex adaptive mechanisms include the transduction of the physical parameters variations into molecular signals. studies in carp have indicated that prolactin and growth hormone expression is associated with acclimatization, suggesting that the pituitary gland is a relevant physiological node in the generation of the homeostatic rearrangement that occurs in this adaptive process. here, ... | 2004 | 15095402 |
| carp virus crisis prompts moves to avert global spread. | 2004 | 14961087 | |
| common carp metallothionein-1 gene: cdna cloning, gene structure and expression studies. | metallothionein-1 (mt-1) cdna clones were isolated from a common carp (cyprinus carpio) uninduced hepatopancreas cdna library. northern blot assay using the common carp (cc) mt-1 cdna as a probe showed high fold induction of ccmt mrna levels in the intestine and kidney following exposure to cd2+ and zn2+. using polymerase chain reaction (pcr), primers designed from the cdna sequences allowed the isolation of ccmt-1 gene fragments including the 5'-flanking region. the 600 bp 5'-flanking region of ... | 2004 | 14746911 |
| debromination of the flame retardant decabromodiphenyl ether by juvenile carp (cyprinus carpio) following dietary exposure. | the congener 2,2',3,3',4,4',5,5',6,6'-decabromodiphenyl ether (bde 209) is the primary component in a commonly used flame retardant known as decabde. this flame retardant constitutes approximately 80% of the world market demand for polybrominated diphenyl ethers (pbdes). because this compound is very hydrophobic (log k(ow) approximately 10), it has been suggested that bde 209 has very low bioavailability, although debromination to more bioavailable metabolites has also been suggested to occur in ... | 2004 | 14740725 |
| comet assay and micronucleus test in circulating erythrocytes of cyprinus carpio specimens exposed in situ to lake waters treated with disinfectants for potabilization. | the detection of a possible genotoxic effect of surface water treated with disinfectants for potabilization is the aim of the present work. the comet assay and the micronucleus test were applied in circulating erythrocytes of cyprinus carpio. young specimens (20-30 g) were exposed in experimental basins, built within the potabilization plant of castiglione del lago (perugia, italy). in this plant the water of the trasimeno lake is treated and disinfected for potabilization before it is distribut ... | 2004 | 14729366 |
| purification and cloning of an endogenous protein inhibitor of carp nephrosin, an astacin metalloproteinase. | nephrosin is a newly discovered member of the astacin family. it is a secreted proteinase and is present in carp head kidney, kidney, and spleen, all of which are responsible for immune and hematopoietic functions in fish. a complex formed by nephrosin and its inhibitor was purified from carp kidney extract by heparin affinity column chromatography. the presence of the nephrosin-inhibitor complex in different tissues was examined by immunoblotting with polyclonal antisera against the purified ne ... | 2004 | 14709555 |
| purification and characterization of myofibril-bound serine protease from lizard fish (saurida undosquamis) muscle. | myofibril-bound serine protease (mbsp) from lizard fish (saurida undosquamis: synodontidae) skeletal muscle was purified to homogeneity with higher purification (1260-fold) and higher recovery (7%) than our previous report in lizard fish (saurida wanieso). the new purification method combines a heat-treatment for dissociation from washed myofibrils, acid-treatment at ph 5.0 before and after lyophilization, and alcohol-treatment, followed by two column chromatographies. the molecular mass of the ... | 2004 | 14698920 |
| molecular characterization of cathepsin l from hepatopancreas of the carp cyprinus carpio. | purified cathepsin l from carp, cyprinus carpio, consists of a 28 kda single-chain form that is different from the 24 and 5 kda mammalian two-chain form. we cloned cathepsin l from carp hepatopancreas. the sequence consisted of a 1490 bp cdna and a 1014 bp open reading frame, encoding a deduced protein of 337 amino acids that is likely processed to an active enzyme (single-chain form) with 222 amino acids. its similarity to other types of vertebrate cathepsin l is less than 69%. mammalian cathep ... | 2004 | 14698917 |
| one-step, non-denaturing purification method of carp (cyprinus carpio) vitellogenin. | a new single-step purification procedure was developed to purify carp (cyprinus carpio) vitellogenin (vtg), from estradiol-treated carp plasma. this method was performed by high performance liquid weak anion-exchange chromatography, using a discontinuous elution gradient of nacl (0-0.5 m, steps of 12.5 mm/4 min). sds and native-page analysis, of treated-fish plasma and purified solution, showed the appearance of a 370 kda phospholipoprotein, composed of two 130 kda monomers, with all vtg charact ... | 2004 | 14659439 |
| development of quantitative vitellogenin-elisas for fish test species used in endocrine disruptor screening. | the yolk protein precursor vitellogenin (vtg) in plasma has proved to be a simple and sensitive biomarker for assessing exposure of fish to environmental estrogens. within international bodies such as the organization for economic cooperation and development (oecd) work is ongoing to develop screening and testing programmes for endocrine disrupting effects of new chemicals, and in the focus of this development are the fish test species common carp (cyprinus carpio), fathead minnow (pimephales pr ... | 2004 | 14551668 |
| use of vitellogenin mrna as a biomarker for endocrine disruption in feral and cultured fish. | the presence of the female-specific yolk protein precursor vitellogenin in blood and liver from male fish is widely used as an indicator of endocrine disruption. we studied the induction of vitellogenin mrna in liver from several species of fish, both maintained in fish tanks or captured in the wild. our procedure requires minute amounts of liver samples (down to 50 mg), and can be applied to field samples if the appropriate rna-stabilisation agent is used. we used reverse-transcriptase pcr and ... | 2004 | 14618290 |
| feeding level and individual metabolic rate affect delta 13c and delta 15n values in carp: implications for food web studies. | stable isotope analyses are often used to calculate relative contributions of multiple food sources in an animal's diet. one prerequisite for a precise calculation is the determination of the diet-tissue fractionation factor. isotopic ratios in animals are not only affected by the composition of the diet, but also by the amount of food consumed. previous findings regarding the latter point are controversial. as stable isotope analyses have often been used to investigate aquatic food webs, an exp ... | 2004 | 14608500 |
| isolation and characterisation of pentraxin-like serum proteins from the common carp cyprinus carpio. | there is increasing economic and ecological interest in the development of assays for the early detection of infection, disease activity and environmental stress in marine and freshwater animals. in humans the serum pentraxin c-reactive protein (crp) is universally used as a clinical indicator of inflammation and underlying infection. as a first step towards assessing the potential of an immunoassay for crp in fish, we have isolated and characterised common carp cyprinus carpio crp and a highly ... | 2004 | 12969797 |
| [use of brain weight in population studies of carp fry]. | 2004 | 499036 | |
| the reserpine effects on the gonadotrophic cells of the male common carp cyprinus carpio (osteichtyes: cyprinidae). | the secretion of gonadotropins (gth) in goldfish and carp, is stimulated by gth-releasing hormone (gnrh) and is inhibited by dopamine. studies with antidopaminergics have demonstrated to be effective in order to stimulate the spermiation and the ovulation in different species of teleosts. the reserpine, a drug that deplets the dopamine, has shown to stimulate the spermiation in the common carp. we report here, the effects of reserpine on the number and volume of gonadotrophic cells of the common ... | 2004 | 17357409 |
| the strengths of in vivo magnetic resonance imaging (mri) to study environmental adaptational physiology in fish. | adaptational physiology studies how animals cope with their environment, even if this environment is subject to permanent fluctuations such as tidal or seasonal variations. aquatic organisms are generally more prone to be exposed to osmotic, hypoxic and temperature challenges than terrestrial animals. some of these challenges are more restraining in an aquatic environment. to date, very few studies have used in vivo magnetic resonance imaging (mri) to uncover the physiological mechanisms that re ... | 2004 | 15614515 |
| reproductive responses of common carp (cyprinus carpio) exposed in cages to influent of the las vegas wash in lake mead, nevada, from late winter to early spring. | the las vegas wash (lw) delivers tertiary-treated municipal wastewater effluent, nonpotable shallow groundwater seepage, and runoff from the urbanized las vegas valley to las vegas bay (lx) of lake mead. to investigate the potential for contaminants in lw influent to produce effects indicative of endocrine disruption in vivo, adult male and female common carp (cyprinus carpio) were exposed in cages for 42-48 d at four sites in lake mead: lw, lx, and two reference locations in the lake. end point ... | 2004 | 15597896 |
| role of ca2+ store in ampa-triggered ca2+ dynamics in retinal horizontal cells. | fura-2 fluorescent calcium imaging was applied to measure [ca(2+)](i) in freshly dissociated horizontal cells of carp retina, and a model containing endoplasmic reticulum (er) membrane processes and plasma membrane processes was constructed for quantitative analyses of the ampa-triggered calcium dynamics. a transient increase followed by a sustained steady level of [ca(2+)](i) was observed when 100 microm ampa was applied, while the initial transient increase of [ca(2+)](i) was suppressed by exo ... | 2004 | 15640746 |
| [the analysis of genetic variability of mtdna 12s rrna genes in the allotetraploid, triploid crucian carp and their parents]. | the complete 12s rrna genes from the five f11 allotetraploids, two diploid gynogens, two triploid crucian carps, one red crucian carp, one xiangjiang common carp, and one japanese crucian carp were successfully cloned and sequenced. sequence compositions and variations were analyzed with mega 1.0 software. five f11 allotetraploids shared two haplotypes, and two diploid gynogens, two triploid crucian carps, one red crucian carp, one xiangjiang common carp, one japanese crucian carp shared one hap ... | 2004 | 15640120 |
| elevated dna-protein cross-links in red blood cells of german carp, cyprinus carpio, from the lower passaic river, new jersey, usa. | 2004 | 15669735 | |
| noise emission during the first powerboat race in an alpine lake and potential impact on fish communities. | in order to assess the effects of high-speed boating on fish communities, noise levels were measured during the first class 1 powerboat race on the austrian lake traunsee. the noise spectra were compared to natural ambient noise and hearing abilities of four native fish species. sound pressure levels (spls) were significantly elevated during the training heats and the race compared with natural levels, reaching up to 128 db re 1 micropa (instantaneous spl) at a distance of 300 m to the powerboat ... | 2004 | 15658729 |
| inhibition of whole body ca2+ uptake in fresh water teleosts, channa punctatus and cyprinus carpio in response to salmon calcitonin. | in many fish, ultimobranchial-derived calcitonin (ct) has been shown to be a potent hypocalcemic regulator. but an equal number of studies failed to show any correlation between ct and plasma calcium levels. again, in fish, where ct has been shown to function as a hypocalcemic hormone, the way this is brought about is not well known. while the regulatory role of many hormones e.g., stanniocalcin, pituitary-derived prolactin and cortisol on gill calcium (ca2+) transport (gcat) has been well estab ... | 2004 | 15673109 |
| partial oxidative-stress perturbs membrane permeability and fluidity of fish nucleated red blood cells. | we investigated the influence of partial oxidative stress on permeability and fluidity of nucleated fish red blood cells for simulating nucleated somatic cells. peroxide value indicating lipid hydroperoxide level in nucleated red blood cells of common carp (cyprinus carpio) increased with increasing body size. we detected that oxidation of nucleated red blood cells led to the degraded pufa compositions and accelerated the permeability of calcein and atp in the nucleated red blood cells restricte ... | 2004 | 15683836 |
| ultrastructural identification of a herpes-like virus infection in common carp cyprinus carpio in korea. | we report on a herpes-like virus, which was found to be associated with mass mortality of common carp cyprinus carpio for the first time in korea in 1998. the external signs of infection in moribund fish were darkened coloration and severe branchial necrosis in the gill. transmission electron microscopy revealed the presence of herpes-like viruses in spleen tissue. infected spleen cells showed hypertrophied nuclei and degeneration. numerous nucleocapsids of about 82 nm in diameter were found wit ... | 2004 | 15584424 |
| a novel serine protease complexed with alpha2-macroglobulin from skeletal muscle of lizard fish (saurida undosquamis). | a novel fish muscle serine protease named muscle soluble serine protease (mssp) was purified from the soluble fraction of lizard fish (saurida undosquamis: synodontidae) muscle by ammonium sulfate fractionation followed by four steps of column chromatographies. in native-page, the purified enzyme appeared as a single band with an estimated mol. mass of approximately 380 kda by gel filtration. in sds-page under reducing conditions, the purified enzyme migrated as two protein bands at 110 and 100 ... | 2004 | 15581796 |
| annual evolution of fatty acid profile from muscle lipids of the common carp (cyprinus carpio) in madagascar inland waters. | annual evolution of muscle lipids fatty acid (fa) from common carp (cyprinus carpio) has been determined in 2001 through monthly samplings in the reserve pond of sisaony (sis series) and itasy lake (ita series) of the madagascar highlands. total lipids from muscle were extracted and quantified according to the bligh and dyer method. fa identification was performed by gc-ms of fa methyl esters and fa pyrrolidides and led to the identification of 41 fa; routine analyses of fa were made by capillar ... | 2004 | 15563217 |
| [cloning and expression analysis in mature individuals of salmon gonadotropin-releasing hormone (sgnrh) gene in common carp]. | two types of complementary dnas (cdna) encoding the precursor of salmon gonadotropin-releasing hormone (sgnrh, [trp7, leu8] gnrh) are cloned and sequenced from common carp brain using rapid amplification of cdna ends (race). the two cdnas are referred to sgnrh cdna1 and cdna2, and the full-length fragment of cdna1 and cdna2 were 393 and 478 bp, respectively. two sgnrh cdnas contain an open reading frame of 285 bp, which encodes the sgnrh precursor including 94 amino acid residues. the sgnrh prec ... | 2004 | 15552041 |
| coping with cold: an integrative, multitissue analysis of the transcriptome of a poikilothermic vertebrate. | how do organisms respond adaptively to environmental stress? although some gene-specific responses have been explored, others remain to be identified, and there is a very poor understanding of the system-wide integration of response, particularly in complex, multitissue animals. here, we adopt a transcript screening approach to explore the mechanisms underpinning a major, whole-body phenotypic transition in a vertebrate animal that naturally experiences extreme environmental stress. carp were ex ... | 2004 | 15550548 |
| temperature and the expression of myogenic regulatory factors (mrfs) and myosin heavy chain isoforms during embryogenesis in the common carp cyprinus carpio l. | embryos of the common carp, cyprinus carpio l., were reared from fertilization of the eggs to inflation of the swim bladder in the larval stage at 18 and 25 degrees c. crna probes were used to detect transcripts of the myogenic regulatory factors myod, myf-5 and myogenin, and five myosin heavy chain (myhc) isoforms during development. the genes encoding myf-5 and myod were switched on first in the unsegmented mesoderm, followed by myogenin as the somites developed. myf-5 and myod transcripts wer ... | 2004 | 15531645 |
| comparative and evolutionary analysis of the cytochrome b sequences in cyprinids with different ploidy levels derived from crosses. | the mitochondrial cyt b genes in the allotetraploid and triploid crucian carp as well as triploid common carp were isolated and completely sequenced. their dna sequences were compared with those derived from the cyt b genes of the red crucian carp, japanese crucian carp, and common carp with mega 1.0 software. phylogenetic analysis revealed the sister relationships between allotetraploid and diploid red crucian carp, between the triploid crucian carp and diploid japanese crucian carp, and betwee ... | 2004 | 15521428 |
| disparate opinions regarding indications for coronary artery revascularization before elective vascular surgery. | despite consensus guidelines, the optimal strategy for preoperative cardiac risk management among patients scheduled for major noncardiac surgery remains controversial. this study assesses current opinion about the role of preoperative coronary revascularization for patients with coronary artery disease scheduled for elective vascular surgery. thirty-one practicing cardiologists recruited from 4 different regions reviewed case records, imaging tests, and coronary angiograms of 12 patients with c ... | 2004 | 15518605 |
| determination of a bound musk xylene metabolite in carp hemoglobin as a biomarker of exposure by gas chromatography-mass spectrometry using selected ion monitoring. | musk xylene (mx) is widely used as a fragrance ingredient in commercial toiletries. identification and quantitation of a bound 4-amino-mx (amx) metabolite was carried out by gas chromatography-mass spectrometry (gc-ms) with selected ion monitoring (sim). detection of amx occurred after the cysteine adducts in carp hemoglobin (hb), derived from the nitroso metabolite, were released by alkaline hydrolysis. the released amx metabolite was extracted into n-hexane. the extract was preconcentrated by ... | 2004 | 15516317 |
| [studies on the ploymorphic of sperm of f2 hybrids of red crucian carp x common carp]. | athe ultrastructures of the sperm of f2 hybrids of red crucian carp x common carp were studied by using scanning and transmission electron microscope. the sperm of the f2 hybrids consisted of head, mid-piece and tail. there was no acrosome at the anterior end of the nuclears, whereas there was a vesicle. the results revealed that there existed obviously ploymorphic in the sperm of f2 hybrids. in the water-like semen from males of f2 hybrids, different sizes of the head of the sperm including hap ... | 2004 | 15511069 |
| fumonisin b1 causes multiple lesions in common carp (cyprinus carpio). | year-1 carp were fed ratios containing 100mg/kg and 10 mg/kg of added fumonisin b1 for 42 days. the experimental and control fish were examined clinically during the experiment and at the end all fish were necropsied and histological changes recorded. blood vessels, liver, exocrine and endocrine pancreas, excretory and haematopoietic kidney, heart and brain were sensitive both to 100 and 10mg/kg of fb1 in the diet and the rodlet cell (rc) frequency was considerably increased in and around damage ... | 2004 | 15503536 |
| carbonic anhydrases: current state of the art, therapeutic applications and future prospects. | carbonic anhydrases (cas, ec 4.2.1.1) are wide-spread enzymes, present in mammals in at least 14 different isoforms. some of these isozymes are cytosolic (ca i, ca ii, ca iii, ca vii, ca xiii), others are membrane-bound (ca iv, ca ix, ca xii and ca xiv), ca v is mitochondrial and ca vi is secreted in the saliva and milk. three cytosolic acatalytic forms are also known (carp viii, carp x and carp xi). the catalytically active isoforms, which play important physiological and patho-physiological fu ... | 2004 | 15499993 |
| impact of some chlorinated pesticides on the haematology of the fish cyprinus carpio and puntius ticto. | the effects of pesticides on blood characteristics and histological changes in erythrocytes of the fish species cyprinus carpio and puntius ticto were studied. the fishes were exposed to sub lethal concentrations of different chlorinated pesticides namely aldrin, dieldrin, ddt, bhc and chlordane for 10, 20 and 30 d in continuous flow-through test. the lc50 values were calculated based on acute toxicity tests and the sublethal doses were arrived at for chronic bioassay studies. results showed an ... | 2004 | 15495970 |
| [polymorphism in intron 2 of crucian carp gh i gene]. | high length and nucleotide polymorphisms in intron2 of gh i gene were detected in 162 individuals,which were from seven wild crucian carp colonies, two goldfish colonies and one fangzheng crucian carp colony. using denaturating polyareylamide gel electrophoresis (dpage) and single-strand conformation polymorphism (sscp), seven length variants and 15 haplotypes were identified in these fishes. the length types and haplotypes diversity was 4.32% and 9.26%, respectively. sequence analysis of the 15 ... | 2004 | 15490876 |
| calcium-binding protein caldendrin and camkii are localized in spinules of the carp retina. | calcium-binding proteins translate the influx of ca(2+) at excitatory synapses into spatiotemporal signals that regulate a variety of processes underlying synaptic plasticity. in the fish retina, the synaptic connectivity between photoreceptors and horizontal cells undergoes a remarkable plasticity, triggered by the ambient light conditions. with increasing light, the synaptic dendrites of horizontal cells form numerous spinules that are dissolved during dark adaptation. the dynamic regulation o ... | 2004 | 15389610 |
| loss of filament-forming ability of myosin by non-enzymatic glycosylation and its molecular mechanism. | carp and scallop myosin and their subfragments (s-1 and rod) were reacted with glucose to investigate the effect of non-enzymatic glycosylation on the functionality of myosin. the filament-forming ability of the myosin rod diminished with the progress of non-enzymatic glycosylation and myosin became soluble in 0.1 m nacl. the inhibition of the self-assembly of myosin molecules occurred chemically as a result of the increase in negative charge repulsion among myosin molecules and, further, physic ... | 2004 | 15388325 |
| immunology-related perturbations induced by copper and chitosan in carp (cyprinus carpio l.). | copper is used in treatment mixtures to control fungal diseases in vineyards. its concentrations are relatively high in some aquatic ecosystems, and the main problem observed in this study was the antioxidant stress induced by this heavy metal. copper toxicologic effects in aquatic organisms have prompted the demand for alternative use of low-toxicity molecules in culture treatments. chitosan is a polymer with antifungal property similar to copper and may be an interesting biopesticide. thus, it ... | 2004 | 15386131 |
| characterization of retinoic acid neuromodulation in the carp retina. | visual sensation in vertebrates starts with the isomerization of 11-cis retinaldehyde into all-trans retinaldehyde. aldehyde dehydrogenases, present in the pigment epithelium and some retinal cells, convert all-trans retinaldehyde into all-trans retinoic acid (at-ra). evidence in the retina and the hippocampus has accumulated, showing that at-ra, besides being a morphogenetic factor, also acts as a neuromodulator. in mature retina, at-ra affects visual processing by acting on gap junctional cond ... | 2004 | 15378613 |
| dietary accumulation and metabolism of polybrominated diphenyl ethers by juvenile carp (cyprinus carpio). | polybrominated diphenyl ethers (pbdes) are hydrophobic organic contaminants with properties and nomenclature similar to polychlorinated biphenyls (pcbs). while much information is available on the bioaccumulation and pharamcokinetics of pcbs, little information is available on pbdes. in this study, juvenile carp were exposed to a diet spiked with a cocktail of four bde congeners (2,4,4'-tribromode [bde 28], 2,2',4,4'-tetrabromode [bde 47], 2,2',4,4',5-pentabromode [bde 99], and 2,2',4,4',5,5'hex ... | 2004 | 15352483 |
| xenobiotic substances such as pcb mixtures (aroclor 1254) and tbt can influence swimming behavior and biotransformation activity (gst) of carp (cyprinus carpio). | different groups of carp were treated with polychlorinated biphenyl (pcb) or tributyltin (tbt), and possible effects of the chemicals on the swimming behavior of the carp were examined using the behavioquant system. by evaluating quantitative behavioral parameters of the animals, it became evident that exposure to high concentrations of chemicals (organotin, 7 microg l(-1), or polychlorinated biphenyl, 22 microg l(-1)) severely affected the carp, causing a significant change in their swimming sp ... | 2004 | 15352262 |
| effects of endocrine disrupters on sex steroid synthesis and metabolism pathways in fish. | the interactions of estrogenic (nonylphenol, dicofol, atrazine), androgenic (organotins, phthalates, fenarimol) and anti-androgenic compounds (vinclozolin, diuron, p,p'-dde) with key enzymatic activities involved in both synthesis and metabolism of sex hormones was investigated. carp testicular microsomes incubated in the presence of androstenedione and different xenobiotics evidenced higher sensitivity of 5alpha-reductase activity than 17beta-hydroxysteroid dehydrogenase activity towards those ... | 2004 | 15698553 |
| first ovarian maturity and recovery response in common carp, cyprinus carpio after exposure to carbofuran. | the present study embodies the observations on duration (45, 75 and 105 days) dependent effects of toxicologically low concentration (16 ppm) of a carbamate pesticide, carbofuran, on first ovarian maturity with correlative recovery response by withdrawal of treatment for 30 days in virgin yearlings of common carp, cyprinus carpio var. communis. in control fish histological organization of ovary and changes in gonado-somatic index (gsi) revealed the initiation of oocyte differentiation and develo ... | 2004 | 15847330 |
| concentrations of arsenic, cadmium, copper, lead, selenium, and zinc in fish from the mississippi river basin, 1995. | fish were collected in late 1995 from 34 national contaminant biomonitoring program (ncbp) stations and 12 national water quality assessment program (nawqa) stations in the mississippi river basin (mrb), and in late 1996 from a reference site in west virginia. the ncbp sites represented key points (dams, tributaries, etc.) in the largest rivers of the mrb. the nawqa sites were typically on smaller rivers and were selected to represent dominant land uses in their watersheds. the west virginia sit ... | 2004 | 15887377 |
| growth and development of catla (catla catla) fed with different levels of diet containing spirogyra sp. | the effect of spirogyra sp. incorporated into diet formulations on the growth and body composition of indian major carp, catla (catla catla) was investigated in a 45 days feeding trial. spirogyra dry powder was mixed with different feed ingredients in different amounts (0%, 10%, 25%, 37% and 40% of the total feed). carps fed with spirogyra demonstrated higher feed conversion ratio. the study also revealed a direct relationship between the amount of spirogyra in the diet, and muscle protein and f ... | 2004 | 15207298 |
| regulation of action potential duration under acute heat stress by i(k,atp) and i(k1) in fish cardiac myocytes. | the mechanism underlying temperature-dependent shortening of action potential (ap) duration was examined in the fish (carassius carassius l.) heart ventricle. acute temperature change from +5 to +18 degrees c (heat stress) shortened ap duration from 2.8 +/- 0.3 to 1.3 +/- 0.1 s in intact ventricles. in 56% (18 of 32) of enzymatically isolated myocytes, heat stress also induced reversible opening of atp-sensitive k+ channels and increased their single-channel conductance from 37 +/- 12 ps at +8 d ... | 2004 | 14592934 |
| maintained cardiac pumping in anoxic crucian carp. | like most vertebrates, humans die within minutes when deprived of molecular oxygen (anoxia), in part because of cardiac failure. in contrast, some freshwater turtles can survive anoxia for months at low temperatures, but to do so, they drastically suppress cardiac activity and autonomic cardiovascular control. although carassius carassius, the crucian carp, shares this anoxia tolerance, we show that it has a unique ability among vertebrates to retain normal cardiac performance and autonomic card ... | 2004 | 15459381 |
| hypoxic survival strategies in two fishes: extreme anoxia tolerance in the north european crucian carp and natural hypoxic preconditioning in a coral-reef shark. | especially in aquatic habitats, hypoxia can be an important evolutionary driving force resulting in both convergent and divergent physiological strategies for hypoxic survival. examining adaptations to anoxic/hypoxic survival in hypoxia-tolerant animals may offer fresh ideas for the treatment of hypoxia-related diseases. here, we summarise our present knowledge of two fishes that have evolved to survive hypoxia under very different circumstances. the crucian carp (carassius carassius) is of part ... | 2004 | 15299034 |
| vertebrate brains at the pilot light. | while the brains of most vertebrates are unable to tolerate more than a few minutes of anoxia, some freshwater turtles (trachemys and chrysemys), crucian carp (carassius carassius) and frogs (rana pipens and rana temporaria) can survive anoxia for hours to months. obviously, anoxia tolerance has evolved separately several times and this is also reflected in the divergent strategies these animals utilize to survive without oxygen. the turtles and crucian carp defend their brain atp levels and avo ... | 2004 | 15288600 |
| seasonality of dihydropyridine receptor binding in the heart of an anoxia-tolerant vertebrate, the crucian carp (carassius carassius l.). | prolonged anoxia tolerance of facultative anaerobes is based on metabolic depression and thus on controlled reduction of energy-utilizing processes. one proposed survival mechanism is the closing of ion channels to decrease energetic cost of ion pumping (hochachka pw. science 231: 234-241, 1986). to test this hypothesis, the involvement of l-type ca2+ channels in seasonal anoxia tolerance of the vertebrate heart was examined by determining the number of [methyl-3h]pn200-110 (a ligand of l-type c ... | 2004 | 15242827 |
| metabolic responses to low temperature in fish muscle. | for most fish, body temperature is very close to that of the habitat. the diversity of thermal habitats exploited by fish as well as their capacity to adapt to thermal change makes them excellent organisms in which to examine the evolutionary and phenotypic responses to temperature. an extensive literature links cold temperatures with enhanced oxidative capacities in fish tissues, particularly skeletal muscle. closer examination of inter-species comparisons (i.e. the evolutionary perspective) in ... | 2004 | 15191230 |
| neurophysiologic detector-a selective and sensitive tool in high-performance liquid chromatography. | in the present study neurons from the olfactory system of the fish crucian carp, carassius carassius l. were used as components in an in-line neurophysiologic detector (npd) to measure physiological activities following the separation of substances by high-performance liquid chromatography (hplc). the skin of crucian carp, c. carassius l. contains pheromones that induce an alarm reaction in conspecifics. extra-cellular recordings were made from neurons situated in the posterior part of the media ... | 2004 | 14698234 |
| [triploid crucain carp-allotetraploid hybrids (male) x goldfish( hermaphrodite)]. | a new type of triploid crucian carp was generated by mating the males of the allotetraploid hybrids of red crucian carp (carassius auratus red var.) (female) x common carp (cyprinus carpio l.) (male), with the females the goldfish( carassius auratus red var.) which was red in color and possessed the biforked tails. the biological characteristics of one-year old triploid crucian carp including the number and karotype of chromosome, gonadosomatic indices and gonadal structures, appearance and grow ... | 2004 | 15468916 |
| immunotoxicity of pentachlorophenol on macrophage immunity and igm secretion of the crucian carp (carassius auratus). | 2004 | 15386086 | |
| [karyotypic analysis from two clones of gynogenetic pengze crucian carp (carassius auratus of pengze)]. | pengze crucian carp (carassius auratus of pengze), one of the most popular cultural fishes in china, is an endemic bisexual population with natural gynogenetic reproduction mode. just like silver crucian carp, investigation on the karyotype of pengze crucian carp is important to understanding the diversity and the evolution of the unisexual vertebrates. two distinguishable gynogenetic clones of pengze crucian carp, named clone h and clone l, respectively,were detected recently. in this study, th ... | 2004 | 15481531 |
| effects of four chlorobenzenes on serum sex steroids and hepatic microsome enzyme activities in crucian carp, carassius auratus. | four chlorobenzenes (chlorobenzene, 1,3-dichlorobenzene, 1,4-dichlorobenzene, p-chloro-methylbenzene) were administrated to the crucian carps (carassius auratus) by peritoneal injections in the laboratory for 30 days. serum testosterone and 17 beta-estradiol concentrations were detected using radioimmunology assay (ria), and the activities of two hepatic microsome enzymes, glutathione s-transferase (gst) and udp-glucuronosyltransferase (udpgt), were measured using the modified methods as describ ... | 2004 | 15294436 |
| identification of a novel c1q family member in color crucian carp (carassius auratus) ovary. | potential roles of c1q/tumor necrosis factor (tnf) superfamily proteins have been observed in vertebrate oogenesis and oocyte maturation, but no ovary-specific member has been identified so far. in this study, we have cloned and identified a novel member of c1q family with a c1q domain in the c-terminal from fully grown oocyte cdna library of color crucian carp and demonstrated that the gene might be specifically expressed in ovary and therefore designated as carassius auratus ovary-specific c1q ... | 2004 | 15253877 |
| cell culture of clonal ginbuna crucian carp hematopoietic cells: differentiation of cultured cells into erythrocytes in vivo. | we cultivated kidney hematopoietic cells from clonal triploid ginbuna crucian carp, carassius auratus langsdorfii. proliferating cells from hematopoietic cell cultures were harvested and injected into tetraploid hybrids (clonal triploid ginbuna and goldfish hybrid) which possess three sets of chromosomes from a triploid clone and a haploid set of chromosomes from goldfish (carassius auratus). after injection of cultured triploid cells (donor cells), blood samples were obtained from tetraploid hy ... | 2004 | 15183027 |
| development of an immunosensor for the detection of vitellogenin using impedance spectroscopy. | a simple and direct immunosensor for the determination of carp (carassius auratus) vitellogenin (vtg), a female-specific protein, has been proposed based on an antibody-captured conducting polymer-coated electrode. the monoclonal antibody specific to carp (c. auratus) vtg was immobilized by covalent coupling to the carboxylic acid group on the polymer. the antibody immobilization and antibody-antigen interaction have been demonstrated by means of quartz crystal microbalance and impedance spectro ... | 2004 | 15046756 |
| pluripotency of cryopreserved blastomeres of the goldfish. | to examine the pluripotency of cryopreserved blastomeres, we transplanted them into blastula. donor blastomeres were prepared from blastula of goldfish (carassius auratus) and cryopreserved in liquid nitrogen for two months. fifty-five percent and 44% of blastomeres survived after thawing. cryopreserved blastomeres were transplanted to the blastula of triploid crucian carp (c. a. longsdorfii), which reproduces gynogenetically in nature. at four days after the operation, resultant chimeric embryo ... | 2004 | 14743512 |
| coexistence of a sexual and an unisexual form stabilized by parasites. | an unisexual species (either parthenogenetic or gynogenetic form) often coexists sympatrically with a closely related anisogamous sexual species, forming a unisexual complex. this is puzzling because, all else being equal, a sexual population with the two-fold cost of sex (or the cost of producing males) cannot coexist with a unisexual population. this suggests that some ecological processes are at work to make possible the coexistence of the unisexual complex. field and laboratory studies sugge ... | 2004 | 14643188 |
| effects of growth hormone on leucine absorption, intestinal morphology, and ultrastructure of the goldish intestine. | the mechanisms whereby exogenous growth hormone modulates intestinal structure and function in fish were investigated. goldfish (carassius auratus) were fed commercial flake diet sprayed with recombinant carp growth hormone (cgh) daily for 1 month. control animals received food sprayed with the vehicle. after 1 month of daily feedings, body mass and length were determined, and animals were sacrificed to study intestinal characteristics. sections of foregut were removed after determination of tot ... | 2004 | 15644934 |