Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| d-alanine and catalase in mycobacterium leprae. | 1988 | 3057085 | |
| [reproduction of experimental leprous infection in mice with a preliminary modelling of failure of the mononuclear phagocyte system]. | cba mice with induced insufficiency of mononuclear phagocyte system (mnps) were inoculated intraplantar with m. leprae in a dose of 1 x 10(4). a significantly accelerated multiplication of m. leprae was noted in the sites of inoculation with the development of a generalized infections process and appearance in 6 months after inoculation of lepromatous structures in spleen, liver and other internal organs as compared with the animals with unchanged mnps. the data obtained suggest that insufficien ... | 1988 | 3058225 |
| [growth of human leprosy bacilli in the foot-pad of mice]. | 1988 | 3058272 | |
| leprosy. | leprosy affects over 10 million people in the world. the disease is a model of graded cell-mediated immunity, in this case to the causative organism, mycobacterium leprae. the clinical manifestations are due to (i) bacterial progression, (ii) immunologic responses of the host, (iii) peripheral nerve damage due to either or both bacterial progression and immunologic responses of the host, and (iv) preventable secondary deformities following nerve damage, which account for most of the stigma of th ... | 1988 | 3058299 |
| benefits of recombinant dna technology for the study of mycobacterium leprae. | 1988 | 3058391 | |
| implications of prior bcg vaccination programmes in the community on the protective efficacy of new antileprosy vaccines. | 1988 | 3058589 | |
| a 28-kda protein from mycobacterium leprae is a target of the human antibody response in lepromatous leprosy. | the gene for a 28-kda mycobacterium leprae protein ag, a major target of antibodies from patients with lepromatous leprosy, was cloned from a lambda-gt11-m. leprae dna expression library and sequenced. antibodies to this protein were detected in the serum of the majority of 15 individual lepromatous patients that were tested. the predicted amino acid sequence of the 28-kda protein suggests that it is localized to the bacterial plasma membrane or cell wall. | 1988 | 3058804 |
| presence of mycobactin-like substance in mycobacterium leprae. | chloroform extracts of m. leprae suspensions--crude, partially purified and purified--were prepared by standard methods. similar extracts were also prepared from the livers of normal armadillos using the same methods that were used to prepare crude and partially purified m. leprae suspensions. the only chloroform extract that supported the growth of m. paratuberculosis was the one prepared from percoll gradient-purified m. leprae. other four extracts not only did not support the growth of mycoba ... | 1988 | 3058826 |
| a bacteriological assessment of multibacillary cases in leprosy colonies after 4 1/2 years of multidrug therapy. | in this presentation we have devised a novel way of calculating the total bacterial quantum in 100 (78 ll and 22 bl) multibacillary leprosy patients living in leprosy colonies. the calculation is based on ridley's logarithmic scale. we have also attempted to assess the reduction in the bacterial quantum as a result of intervention through multidrug therapy (mdt). 53% of the patients rendered bacteriologically negative within two years of treatment of mdt and 94% at 54th pulse dose i.e. at 54th m ... | 1988 | 3058827 |
| influence of acetylator phenotype of the leprosy patient on the emergence of dapsone resistant leprosy. | the half time of disappearance of dapsone and monoacetyl dapsone and the acetylator phenotype of the leprosy patients who harboured dapsone sensitive and dapsone resistant m. leprae was assessed in 27 subjects. sixteen patients were rapid acetylators, five were slow and six were intermediate acetylators. the mean t 1 1/2 lives of dapsone (30.26 +/- 11.0) and monoacetyl dapsone (31.11 +/- 12.0) were also studied in the above patients. the percentage of different acetylators in both resistant and ... | 1988 | 3058828 |
| filamentous phase in life cycle of m. leprae(?): a preliminary communication. | certain structures which indicate probable involvement of a filamentous phase in life cycle of m. leprae have been noted in preserved skin biopsy suspensions from lepromatous leprosy cases. these include (i) filaments with empty or pink round spaces within them (ii) conidia-like structures and (iii) membranes with acid fast bacilli. these structures were rare in the fresh material. | 1988 | 3058829 |
| paucibacillary primary dapsone resistance--a case report. | a case of primary paucibacillary dapsone resistance was presented. its clinical suspicion and diagnosis are stressed. emergence of dapsone resistance and its implications are shortly reviewed. a short note on its prevention is discussed. literature is briefly reviewed. | 1988 | 3060544 |
| secondary and primary dapsone resistant leprosy: an analysis of 199 patients from st. thomas hospital and leprosy project, chettupattu, south india. | the occurrence of secondary and primary dapsone resistance in 199 patients in our control area and the influence of certain variables such as age, initial bacteriological and morphological indices, duration of regular dapsone monotherapy, on the emergence of dapsone resistance was investigated. ninety one of 122 patients and 29 out of 77 showed secondary (sdr) and primary (pdr) resistance to dapsone respectively. very low bi (bi:2.5) group also showed both sdr (60%) and pdr (40%). low or high mi ... | 1988 | 3060545 |
| armadillos in an indian leprosy research centre. | 1988 | 3060547 | |
| primary drug resistance to both rifampicin and dapsone in a paucibacillary leprosy patient. | a thirty-one year old male patient was diagnosed and treated for a pure or better primary neuritic case of leprosy with dapsone (100 mg daily for 2 years) and rifampicin (600 mg daily for 6 months). from the very outset, the patient did not show any improvement; on the top of it, he subsequently, developed a cutaneous patch, which on histopathological examination, revealed classical features of bt leprosy. acid-fast bacilli were absent both in skin slit smear and histologic section. a primary re ... | 1988 | 3060548 |
| [a summary of leprology]. | 1988 | 3061274 | |
| chemical synthesis of an artificial antigen containing the trisaccharide hapten of mycobacterium leprae. | the trisaccharide allyl o-(3,4-di-o-methyl-beta-d-glucopyranosyl)-(1----4)-o-(2,3-di-o-methyl-al pha-l- rhamnopyranosyl)-(1----2)-3-o-methyl-alpha-l-rhamnopyranoside was synthesized from partially methylated monosaccharide derivatives. condensation of 1,4-di-o-acetyl-2,3-di-o-methyl-alpha-l-rhamnopyranose promoted by boron trifluoride etherate with the appropriate alcohol proceeded stereoselectively and with very high yields. selective deacetylation and glycosylation with 2,4-di-o-acetyl-3,6-di- ... | 1988 | 3063382 |
| synthesis and immunoreactivity of neoglycoproteins containing the trisaccharide unit of phenolic glycolipid i of mycobacterium leprae. | the trisaccharide segment, o-(3,6-di-o-methyl-beta-d-glucopyranosyl)-(1----4)-o-(2,3-di-o-methyl- alpha-l-rhamnopyranosyl)-(1----2)-3-o-methyl-l-rhamnopyranose, of the mycobacterium leprae-specific phenolic glycolipid i has been synthesized as its 8-(methoxycarbonyl)octyl glycoside and coupled to a carrier protein, to produce a leprosy-specific neoglycoprotein, the so-called natural trisaccharide-octyl-bovine serum albumin (nt-o-bsa). special features of the synthetic strategy were the use of si ... | 1988 | 3063383 |
| the status of leprosy control in malaysia. | 1988 | 3064325 | |
| the present status of leprosy vaccine development. | 1988 | 3064326 | |
| use of anti-m. leprae phenolic glycolipid-i antibody detection for early diagnosis and prognosis of leprosy. | untreated patients suffering from tuberculoid, lepromatous and indeterminate leprosy, their domiciliary contacts, and healthy controls, all living in guadeloupe, west indies, were tested by an elisa for detecting igm antibodies to the terminal disaccharide of the phenolic glycolipid-i antigen of mycobacterium leprae. on most subjects, a mitsuda test was also performed. a large majority of the tuberculoid patients and healthy subjects were mitsuda positive. the seropositivity rate reached 44% amo ... | 1988 | 3065419 |
| in vitro synthesis of antimycobacterial antibodies with different specificities in various tissues of leprosy patients. | for the detection of the synthesis in vitro of anti-mycobacterium leprae antibodies in various tissues of leprosy patients, biopsy specimens of skin lesions, nasal mucosa, larynx, lymph nodes, and bone marrow were cultured in a medium containing 14c-labeled lysine and isoleucine. the culture fluids were analyzed by crossed immunoelectrophoresis with intermediate gel and autoradiography. the results show that synthesis of anti-m. leprae antibodies occurs at the investigated sites of leprosy patie ... | 1988 | 3065420 |
| effects of freezing and thawing on the viability and the ultrastructure of in vivo grown mycobacteria. | the influence of different frequencies of freezing-thawing cycles on the viability of in vivo grown mycobacteria was investigated. pieces of armadillo tissues naturally or experimentally infected with mycobacterium leprae were analyzed. the viability of m. leprae was determined by mouse foot pad titration. the viability of cultivable mycobacteria, sometimes present in armadillo tissues, was determined by culture. electron-microscopic studies were performed on fresh or frozen-thawed armadillo tis ... | 1988 | 3065421 |
| inhibition of phenolic glycolipid-i synthesis in extracellular mycobacterium leprae as an indicator of antimicrobial activity. | the effects of 22 antimicrobial agents on the incorporation of [u14c] palmitic acid ([u14c] pa) into the unique phenolic glycolipid-i (pgl-i) antigen of mycobacterium leprae were studied. nude-mouse-propagated m. leprae were incubated in a modified dubos medium in the presence of antimicrobial agents for 4 days. [u14c] pa was then added and incubation was continued for 8 days. the antileprosy agents dapsone, rifampin, and clofazimine (2 micrograms/ml each) caused a significant reduction in [u14c ... | 1988 | 3065422 |
| search for leprous infection in some small wild animals of louisiana. | 1988 | 3065423 | |
| psychrophilic mycobacteria in m. leprae-infected tissues. | 1988 | 3065424 | |
| multiplication of armadillo-derived m. leprae in murine dissociated schwann cell cultures. | 1988 | 3065425 | |
| purification and characterization of a 36 kda antigen of mycobacterium leprae. | a 36 kda antigen of mycobacterium leprae was purified by phenol biphasic partition followed by preparative sds-page. the purified antigen appeared as a single band in sds-page and eluted as a single peak in ion-exchange chromatography. the antigen comprised epitopes which were cross-reactive with m. tuberculosis, as well as a species-specific epitope (recognized by mab f47-9). different treatments of the 36 kda antigen suggested it to be largely protein in nature; the amino acid composition of 8 ... | 1988 | 3065448 |
| [viruses in multiple sclerosis]. | 1988 | 3066017 | |
| accessory cell function of dendritic cells from lymph nodes containing mycobacterium leprae induced granulomas. | dendritic cells were enriched from guinea pig auricular lymph nodes containing mycobacterium leprae induced granulomas by immunomagnetic depletion of other cells. these cells were strongly positive for major histocompatibility complex class ii antigens and labelled with an antidendritic cell monoclonal antibody, but not with an antimacrophage antibody. interdigitating dendritic cells were identified in the granulomatous lymph node by staining with the antidendritic cell antibody and by transmiss ... | 1988 | 3068154 |
| recognition of mycobacterial antigens by sera from patients with leprosy. | mycobacterium leprae sonic extracts prepared from armadillo-derived bacteria were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting (immunoblotting) procedures and probed with serum or plasma samples from 20 patients with lepromatous leprosy and 14 healthy endemic controls. five proteins of 33, 25, 18, 15, and 12 kilodaltons (kda) were frequently recognized; the 33- and 15-kda proteins were, respectively, recognized with high intensity by 16 and 13 of th ... | 1988 | 3068245 |
| demonstration of acid-fast bacilli in skin biopsies from indeterminate leprosy cases. | because the correct diagnosis of indeterminate leprosy (il) requires the finding of acid-fast bacilli in skin lesions from clinically and histopathologically suggestive cases, it is necessary to develop a reliable method for this purpose. this paper presents a simple procedure, available to every general laboratory, which consists in obtaining 2 suspensions: si, by mincing and grinding the tissue in phosphate-buffered saline; and sii, after treating si with naoh solution and digesting with tryps ... | 1988 | 3068861 |
| the efficacy of a cell-mediated reaction in the disposal of m. leprae in human skin. | the inability of lepromatous leprosy patients to mount a cellular immune response against mycobacterium leprae antigens is not understood. the extensive intracellular replication of bacilli in the phagocytes and the relative paucity of t lymphocytes in the lesions suggest that these patients might be incapable of generating normal delayed type hypersensitivity responses in their skin. in order to elucidate this problem we evaluated the patient's response to local antigen administration. our obse ... | 1988 | 3069709 |
| lepromin conversion induced by a 'sub-unit' vaccine from icrc bacilli. | 1988 | 3071521 | |
| particular matrix for fast atom bombardment mass spectrometric analysis of phenolic glycolipid antigens isolated from pathogen mycobacteria. | phenolic glycolipids play a key role as an antigenic probe for serodiagnosis of some human pathogen mycobacterial infections. the lipidic part which corresponds to a phenolphthiocerol dimycocerosate molecule, and the presence of partial o-methylated sugars, confer a high hydrophobicity to this kind of molecule. fast atom bombardment (fab) mass spectrometric analysis with standard matrices such as glycerol or thioglycerol was unsuccessful. using a new matrix--monobutyltriethylene glycol--fab anal ... | 1988 | 3072032 |
| monitoring of bacterial drug response by mass spectrometry of single cells. | the application of the laser microprobe mass analyser lamma 500 to the solution of problems in the field of microbiology is reported. the special features of this instrument allow the analysis of single bacterial cells, and questions can be answered which are not accessible to the normally applied integral methods. thus it is possible to establish distributions of, for instance, elemental concentrations within a bacterial population and of correlations between measured characteristics of a bacte ... | 1988 | 3072034 |
| dermal characteristics of the hereditary hairless-athymic mouse and the multiplication at early stage of mycobacterium leprae inoculated into the right hind foot. | 1988 | 3072329 | |
| [epidemiological immunological studies on leprosy in okinawa. iii. reactivity to the standard and diluted lepromins of leprosy patients in the mainland and okinawa]. | 1988 | 3072330 | |
| mucosal immunity to mycobacteria in leprosy patients and their contacts. | 1988 | 3072455 | |
| use of soluble antigens in leprosy epidemiology. | 1988 | 3072456 | |
| a concentration method for detection and quantitation of bacillaemia in leprosy and its comparison with other techniques. | 1988 | 3072457 | |
| slit-skin smears from the fingers in leprosy. | 1988 | 3072458 | |
| in vitro and in vivo activities of macrolides against mycobacterium leprae. | we previously demonstrated the potent in vitro activity of erythromycin against mycobacterium leprae as determined by its effect on atp pools and rates of palmitate oxidation and phenolic glycolipid i synthesis. in the present study, the relative in vitro activities of a number of new macrolides with superior pharmacokinetic properties were assessed. in addition, for the most active compounds, concentrations in serum were determined by bioassay during continuous administration in the feed of mic ... | 1988 | 3072920 |
| the effects of chemotherapy on antibody levels in lepromatous patients. | 1988 | 3073284 | |
| the influence of antimycobacterial chemotherapy on delayed hypersensitivity skin-test reactions in leprosy patients. | 1988 | 3073285 | |
| rifampicin monotherapy in paucibacillary leprosy. | 1988 | 3073286 | |
| inefficacy of thalidomide on rat adjuvant-induced arthritis and lack of arthrogenicity of hansen bacilli. | 1988 | 3074967 | |
| demonstration of acid fast bacilli in the circulating immune complex of leprosy cases and contacts. | 1988 | 3075597 | |
| correlation of in vitro fc receptor assay with in vivo mouse foot pad method. | resistant strains of m. leprae have been reported to the various antileprosy drugs. there is currently no accepted test to identify the susceptibility pattern of m. leprae to the drugs in a short period. the only accepted test is the mouse foot method which takes a long period to yield results. the fc receptor assay using the ability of viable m. leprae to alter the membrane of the macrophage is well established. it takes only ten days and is inexpensive. in 6 cases of leprosy patients the susce ... | 1988 | 3075631 |
| detection of leprosy antigenuria through dot-elisa. | fifty-five samples of urine from different grades of leprosy patients and normal persons were processed for detection of pgl-1 antigen through dot-elisa on nitrocellulose paper strips using anti-human igg horse raddish peroxidaseconjugate. about 66.6% of the paucibacillary and 100% of the multibacillary leprosy cases were detectable through this technique on the basis of differential colour development on the strips. possibility of its use in the field conditions has been discussed. | 1988 | 3075632 |
| bacillaemia in leprosy: correlation with slit-skin and nasal smears. | twenty five multibacillary patients (bl/ll) were studied for bacillaemia. majority (76%) showed acid fast bacilli in peripheral blood. there was good correlation between bacillary load in peripheral blood and bacteriological index (bi) but poor correlation with morphological index (mi) of skin slit smear and bi/mi of nasal smear. | 1988 | 3075633 |
| detection of lepromatous leprosy patients shedding m. leprae in nasal droppings by enzyme immunoassay. | enzyme immunoassays (eias) for detection of lepromatous leprosy (ll) patients harbouring m. leprae in nasal mucosa are described. one eia measures igm antibodies against the synthetic disaccharide (nd-bsa) residue of phenolic glycolipid i of m. leprae, whereas the other titrates primarily igg antibodies against sonicate supernatant antigens of mycobacterium w. (m.w.). fifty coded leprosy sera were analysed by eias under a double blind code. amongst the 20 ll patients with positive nasal smear, 1 ... | 1988 | 3075634 |
| reliability of direct skin smear microscopy in leprosy. | skin smear direct microscopy is an important tool for diagnosis of leprosy. the study was planned to understand the reproducibility of skin smear reading by a trained technician. skin smears were collected from known patients of leprosy from the field area. they were stained for acid fast bacilli following the standard cold staining procedure and were read following the ridley scale. a sample of smears was re-examined on two occasions by the same technician, following blind procedure. there was ... | 1988 | 3075636 |
| use of non-conventional antigen: m. habana in detecting m. leprae antibodies from leprosy patients and contacts in fla-abs test. | an indirect immunofluorescent (fla-abs) test has been developed to detect m. leprae specific antibodies in the active and subclinical cases of leprosy. an antigenically related mycobacterium, m. habana, was used as an antigen to detect m. leprae specific antibodies in the sera samples of leprosy patients. a comparison was made with m. leprae antigen using same set of sera samples. m. habana is capable of detecting anti-m. leprae antibodies in the serum samples of leprosy patients, previously abs ... | 1988 | 3075637 |
| use of carbon sources for lipid biosynthesis in mycobacterium leprae: a comparison with other pathogenic mycobacteria. | carbon from glycerol and palmitate, but not significantly from five other carbon sources tested, was incorporated into lipids by suspensions of non-growing mycobacterium leprae organisms. however, of the five other substrates three-citrate, glucose and pyruvate-were taken up. nongrowing mycobacterium microti and mycobacterium avium incorporated carbon into lipids from most simple carbon sources tested unless they were obtained from growth media including palmitate or from experimentally infected ... | 1988 | 3075652 |
| a component of mycobacterium leprae as a serodiagnostic tool for leprosy. | 1988 | 3076580 | |
| leprosy in malawi. 2. background, design and prospects of the karonga prevention trial, a leprosy vaccine trial in northern malawi. | the karonga prevention trial is the largest vaccine trial ever mounted in africa. it is designed to test two hypotheses: (i) whether the addition of killed mycobacterium leprae can increase the protection against leprosy (and tuberculosis) imparted by bcg alone; and (ii) whether repeating a bcg vaccination can increase upon the protection provided by an initial dose. the rationale and design of the trial are discussed in relation to the history of vaccination against leprosy and to several conte ... | 1988 | 3076995 |
| the experimental inoculation with leprosy bacillus in various hybrid nude mice (continued): the results of cd-1(icr) hybrid nude mice. | 1988 | 3077621 | |
| [cultivation trial of mycobacterium leprae by transformation with deoxyribonucleic acid from cultivable acid-fast bacilli]. | 1988 | 3077623 | |
| successful preparations and characterizations of monoclonal antibodies to a 65,000 dalton protein of mucobacterium leprae. | 1988 | 3077624 | |
| [the malta project (a program for the eradication of leprosy)]. | 1988 | 3078224 | |
| stress-induced proteins and the immune response to leprosy. | mycobacterial antigens which play a major role in humoral and cell-mediated immune recognition are related to proteins which are induced in response to stress stimuli. the potential involvement of stress-induced proteins in infectious disease and in autoimmune responses is discussed. | 1988 | 3079230 |
| pathogenesis of a third world disease: lessons from leprosy. | 1988 | 3079280 | |
| [mycobacteria isolated from armadillos dasypus novemcinctus inoculated with mycobacterium leprae]. | the authors report the isolation of two mycobacteria from armadillos livers and spleens, inoculated with mycobacterium leprae in the kato medium. they discuss this results. | 1988 | 3079304 |
| specificity of mycobacterium tuberculosis phenolic glycolipid (pgl-tb1) antiserum. | the specificity of mycobacterium tuberculosis anti-pgl-tb1 antiserum prepared in rabbits was evaluated by an enzyme-linked immunosorbent assay. it was found that the antiserum immunoreacted with its homologous antigen but not with purified phenolic glycolipids from m. bovis bcg (mycoside b), m. kansasii (mycoside a), m. leprae (pgl-i) or m. marinum (mycoside g). the analysis of chloroform/methanol extracts from 15 strains of m. tuberculosis showed that all formed the antigen except the type stra ... | 1988 | 2472828 |
| the recombinant 65-kd heat shock protein of mycobacterium bovis bacillus calmette-guerin/m. tuberculosis is a target molecule for cd4+ cytotoxic t lymphocytes that lyse human monocytes. | since little is known about tc cells in the human immune response to intracellular parasites, we have studied the role of tc cells in response to m. bovis bacillus calmette-guerin (bcg). donors whose pbmc responded to bcg, purified protein derivative (ppd), and the recombinant 65-kd heat shock protein (hsp) of bcg generated bcg/ppd-specific cd4+ effector t lymphocytes that lysed ppd as well as recombinant 65-kd-pulsed monocytes. nonpulsed or irrelevant antigen-pulsed target cells were lysed to a ... | 1988 | 2903217 |
| the classical and alternate pathways of t cell activation are impaired in leprosy. | the proliferative response of circulating t lymphocytes from bacterial index-positive lepromatous patients to mitogenic anti-cd3 and pairs of anti-cd2 monoclonal antibodies was significantly reduced. in these patients, the cd2 but not cd3 receptor expression was down-regulated. further, the cd2 modulation and the associated suppression of proliferative response to monoclonals was brought about in t cells of healthy subjects by prior incubation of mononuclear cells in vitro with mycobacterium lep ... | 1988 | 2903849 |
| implications of genetics for the epidemiology and control of leprosy. | this paper reviews the rationale and history of genetic studies related to leprosy, and considers their implications for the epidemiology and control of the disease. a long tradition of genetic studies in leprosy was initiated by early impressions that the disease clusters within families. investigations were first motivated by an attempt to understand population patterns, and the focus shifted from investigations of racial differences to investigations of families, of twins and ultimately of ge ... | 1988 | 2907149 |
| leukocyte subsets in the granulomatous response produced after inoculation with mycobacterium leprae-bcg in lepromatous patients. | leukocyte subsets present in the granulomatous response produced after the inoculation of a mixture of mycobacterium leprae and bcg in lepromatous leprosy patients were characterized in situ using monoclonal antibodies and an immunoperoxidase technique. the granuloma produced after m. leprae-bcg inoculation showed a distribution pattern similar to tuberculoid granulomas. t lymphocytes bearing the cd8 phenotype (t cytotoxic/suppressor) were sequestered to the periphery of the epithelioid tubercle ... | 1988 | 2908581 |
| structure of mycobacterial antigens. | 1988 | 2476193 | |
| induction of unresponsiveness to gamma interferon in macrophages infected with mycobacterium leprae. | we have previously demonstrated that mycobacterium leprae-burdened granuloma macrophages isolated from infected nude mice are refractory to activation by gamma interferon (ifn-gamma). to explore further both the afferent and efferent functional capacity of m. leprae-infected macrophages, we examined the ifn-gamma-mediated activation of resident mouse peritoneal macrophages infected in vitro with live or dead m. leprae. when ifn-gamma was administered within 24 h of m. leprae infection, macrophag ... | 1988 | 2840398 |
| nerve damage induced by mycobacterial granulomas in guinea pig sciatic nerves. | a possible model for nerve damage in leprosy has been developed in the sciatic nerve of the guinea pig. intraneural injection of 10(7) bcg organisms into an unsensitized animal induces an epithelioid cell granuloma in 2 weeks similar to that found in tuberculoid leprosy patients. in contrast, intraneural injection of 10(9) cobalt-irradiated mycobacterium leprae organisms induces a macrophage granuloma in 5 weeks, similar to that found in lepromatous leprosy patients. histological, immunohistoche ... | 1988 | 2842415 |
| [the critical study of vaccine therapy of leprosy. the active life of mycobacteria in the long development cycle]. | 1988 | 2845696 | |
| ultrastructural studies of peripheral nerves in lepromatous leprosy patients. | ultrathin sections of the peripheral nerves taken from three lepromatous leprosy patients (one untreated, other treated and third in enl reaction) was examined in the electronmicroscope. in the untreated patient, solid m. leprae organism inside the schwann cell and the degeneration of schwann cell was seen. in contrast, the treated patient showed the degeneration of bacilli and myelinated fibres. however, the characteristics of cells in the enl reaction showed close similarities with the untreat ... | 1988 | 2848907 |
| in vitro methods for determination of viability of mycobacteria: comparison of atp content, morphological index and fda-eb fluorescent staining in mycobacterium leprae. | 1988 | 2469924 | |
| serologic responses to mycobacterium leprae-specific phenolic glycolipid-i antigen in sooty mangabey monkeys with experimental leprosy. | four pairs of sooty mangabey monkeys (cercocebus atys) were inoculated with serial, 10-fold dilutions of mycobacterium leprae. the highest-dose pair received 4.8 x 10(10) m. leprae. serum samples were obtained and clinical signs of leprosy were recorded at intervals of 35 months. longitudinal serum samples were assayed by an elisa method for the presence of igg and igm antibodies to the m. leprae-specific phenolic glycolipid-i (pgl-i) antigen. in general, the onset of disease symptoms paralleled ... | 1988 | 2464657 |
| partial nucleotide sequence of 16s ribosomal rna isolated from armadillo-grown mycobacterium leprae. | ribosomal rna (rrna) was isolated from mycobacterium leprae recovered from infected tissue of the nine-banded armadillo, and nucleotide sequences near the 3' end of the 16s species were determined by primer extension in the presence of dideoxynucleotides. previously published data for bacterial 16s rrnas show a pattern of conserved and non-conserved sequences that fit a common secondary structure. our data for m. leprae fits this general pattern. | 1988 | 2464663 |
| histopathologic observations on the persistence of mycobacterium leprae in the skin of multibacillary leprosy patients under chemotherapy. | 1988 | 2468983 | |
| t cell epitopes on the 36k and 65k mycobacterium leprae antigens defined by human t cell clones. | to identify the molecular localization and specificity of mycobacterium leprae antigenic determinants inducing t cell activation, we studied the reactivity of m. leprae-reactive t cell clones from two tuberculoid leprosy patients towards a battery of different mycobacterial strains and purified mycobacterial antigens. of the 38 t cell clones tested 8 appeared to be m. leprae specific (specificity a), another 8 were cross-reactive with at least one of the three mycobacterial strains, m. lepraemur ... | 1988 | 2454825 |
| specific lysis by cd8+ t cells of schwann cells expressing mycobacterium leprae antigens. | in vitro cultured murine schwann cells which were devoid of class i and class ii gene products of the major histocompatibility complex expressed class i, though not class ii, antigens after stimulation with recombinant interferon-gamma. recombinant ifn-gamma-stimulated schwann cells after priming with m. leprae were lysed by antigen-specific cd8+ t lymphocytes in vitro. these findings suggest that specific lysis of m. leprae-infected schwann cells by cd8+ ctl plays a role in leprosy. | 1988 | 2454827 |
| exact definition of species-specific and cross-reactive epitopes of the 65-kilodalton protein of mycobacterium leprae using synthetic peptides. | with the use of solid phase synthesis of peptides corresponding to major and minor peaks in a hopp-woods hydrophilicity plot, the epitopes for 10 of 14 known different mab to the mycobacterium leprae 65-kda protein, a prominent t and b cell ag of this bacillus, have been located in the primary structure. five epitopes have been precisely mapped by using the synthetic peptides in inhibition elisa experiments, and five others have been located on peptides of 22 amino acids or less in length. the e ... | 1988 | 2454997 |
| a passive hemagglutination test for leprosy using a synthetic disaccharide antigen. | there is a need for a simple, sensitive, and specific test for the serodiagnosis of leprosy. a passive hemagglutination (pha) test for leprosy was developed to meet these requirements. a synthetic disaccharide, conjugated to bovine serum albumin and specific for the phenolic glycolipid of mycobacterium leprae, was sensitized to aldehyde preserved and tanned sheep erythrocytes (srbc). the sensitized srbc were used for testing sera from leprosy and tuberculosis cases and normal controls at 1: 64 a ... | 1988 | 2457641 |
| molecular-genetic evidence for the relationship of mycobacterium leprae to slow-growing pathogenic mycobacteria. | a total of 1170 nucleotides of the 16s rrna from mycobacterium leprae were compared to the homologous regions of m. tuberculosis, m. bovis vallée, m. avium, m. scrofulaceum, m. phlei, m. fortuitum and one representative each of the genera corynebacterium, nocardia, and rhodococcus. homology values were calculated and a phylogenetic tree was constructed from the evolutionary distance values. despite differences in dna g + c content and genome size, m. leprae is a true member of the slow-growing p ... | 1988 | 2458422 |
| evidence that the mitsuda reaction to mycobacterium leprae can be mediated by lymphocytes responsive to mycobacterium tuberculosis. | a positive mitsuda skin test for delayed type hypersensitivity to mycobacterium leprae is associated with a high level of protection against lepromatous leprosy, while the value of tuberculin sensitivity in leprosy is less pronounced. cutaneous lymphocytes, isolated from the mitsuda reaction of a ppd-positive individual not previously exposed to m. leprae, were cloned with dharmendra lepromin and analysed for antigen specificity. thirteen lepromin-responsive cell lines were derived, with greater ... | 1988 | 2458870 |
| gene isolation with human t lymphocyte probes. isolation of a gene that expresses an epitope recognized by t cells specific for mycobacterium bovis bcg and pathogenic mycobacteria. | we have used human cd4+ t lymphocyte clones as primary probes to identify and isolate lambda gt11 rdna clones that express epitopes recognized by t cells. the method that we describe here permits a direct survey of t cell epitope coding sequences in genomic dna or cdna libraries. a lambda gt11 library of mycobacterium leprae dna was screened with m. leprae-reactive human t cell clones as probes, allowing the isolation of a m. leprae dna clone encoding the unidentified ag. this dna clone differs ... | 1988 | 2459226 |
| epitopes of the mycobacterium tuberculosis 65-kilodalton protein antigen as recognized by human t cells. | a synthetic peptide approach has been used to identify the epitopes recognized by clonal and polyclonal human t cells reactive to the recombinant mycobacterial 65-kda protein ag. three of the four epitopes identified were recognized as cross-reactive between mycobacterium tuberculosis and mycobacterium leprae, although their amino acid sequence in two of three cases was not identical. the peptide (231-245) defining an epitope recognized as specific to the m. tuberculosis complex contains two sub ... | 1988 | 2459228 |
| a mycobacterium leprae-specific human t cell epitope cross-reactive with an hla-dr2 peptide. | mycobacterium leprae induces t cell reactivity and protective immunity in the majority of exposed individuals, but the minority that develop leprosy exhibit various types of immunopathology. thus, the definition of epitopes on m. leprae antigens that are recognized by t cells from different individuals might result in the development of an effective vaccine against leprosy. a sequence from the 65-kd protein of this organism was recognized by two hla-dr2-restricted, m. leprae-specific helper t ce ... | 1988 | 2459778 |
| [bacterial sampling using lymph node puncture-aspiration. a study of 50 patients with leprosy]. | fine needle aspiration of lymph nodes was performed in 50 leprosy patients and was compared with usual techniques of bacillary smears taken from nasal mucosa, ear lobule and cutaneous lesion we found that the former was more sensible (30%) regarding bacteriologic and morphologic (34%) indices; moreover this proceeding was more sensible (50%) en patients with type ii leprosy reaction. after 6 month-multi-therapy schedule in 3 patients, the morphologic index decreases 2 points (sfg) and bacteriolo ... | 1988 | 2459893 |
| a comparative evaluation of serological assays for lepromatous leprosy. | 1988 | 2462146 | |
| auramine staining in detecting small number of bacilli in skin smears. | auramine staining has been compared with ziehl-neelsen's staining of m. leprae in skin smear slide. the auramine method was found to be more sensitive than ziehl-neelsen's method and may be useful in detecting small number of m. leprae in skin smears. the inter-observer variance was minimal with auramine staining. | 1988 | 2462604 |
| antigenic proteins of mycobacterium leprae. complete sequence of the gene for the 18-kda protein. | recombinant clones expressing antigenic determinants of the 18-kda protein antigen from mycobacterium leprae recognized by the l5 monoclonal antibody were isolated from a lambda gt11 expression library and their nucleotide sequences determined. all clones expressed the m. leprae-specific determinant as part of a large fusion protein with escherichia coli beta-galactosidase. the deduced amino acid sequence of the coding region indicated that all the lambda gt11 recombinant clones contained an inc ... | 1988 | 2447183 |
| [recent advances in hansen's disease]. | 1988 | 2453134 | |
| use of recombinant antigens expressed in escherichia coli k-12 to map b-cell and t-cell epitopes on the immunodominant 65-kilodalton protein of mycobacterium bovis bcg. | in gene libraries of mycobacterium bovis bcg, mycobacterium tuberculosis, and mycobacterium leprae, recombinants were frequently encountered that expressed an immunodominant 65-kilodalton (kda) protein antigen that was shown to react with a high proportion of mycobacterium-reactive human and murine t cells and murine monoclonal antibodies. in this study, recombinant antigens were used to map t-cell and b-cell epitopes on the m. bovis bcg 65-kda protein that was previously designated mbaa. four d ... | 1988 | 2453469 |
| defective activation of granuloma macrophages from mycobacterium leprae-infected nude mice. | we have previously demonstrated that granuloma macrophages from the foot pads of mycobacterium leprae-infected nude mice are functionally normal despite heavy intracellular burdens of bacilli. however, unlike peritoneal macrophages, these macrophages fail to restrict the intracellular growth of toxoplasma gondii when stimulated with recombinant murine gamma interferon (mu ifn-gamma) and thus appear defective in their response to macrophage-activating factor(s). in further characterizing this def ... | 1988 | 2826628 |
| the effect of phenolic glycolipid-1 from mycobacterium leprae on the antimicrobial activity of human macrophages. | purified pgl-1 and dpgl from m. leprae can prevent bacterial killing by intact phagocytes and cell-free antimicrobial systems. both glycolipids completely abolished the antimicrobial effect of the acetaldehyde-xo-fe2+ system. because the cytotoxicity of this system is inhibited by catalase, sod, mannitol, and ethanol, but not by heated sod or catalase, these data suggest that toxicity is due to oh. generated by the haber-weiss reaction. that the antimicrobial killing in the xo system is complete ... | 1988 | 2826638 |
| dna methylation in leprosy-associated bacteria: mycobacterium leprae and corynebacterium tuberculostearicum. | the dnas of two kinds of microorganisms from human leprosy lesion, mycobacterium leprae and corynebacterium tuberculostearicum (also known as "leprosy-derived corynebacterium" or ldc), have been analysed and compared with the genomes of reference bacteria of the cmn group (genera corynebacterium, mycobacterium and nocardia). the guanine-plus-cytosine content (% gc) of dna was determined by a double-labelling procedure, which is unaffected by the presence of modified and unusual bases (that alter ... | 1988 | 2828900 |
| reduction of tellurite and deesterification of fluorescein diacetate are not well correlated with the viability of mycobacteria. | both mycobacterium leprae and m. lepraemurium (mlm) were capable of reducing tellurium as tellurite ion (te4+) to elemental tellurium (te), seen by electron microscopy as fine crystals within the bacterial cells. there appeared to be close correspondence between the capacity to reduce tellurite, bright green fluorescence after staining with fluorescein diacetate (fda) and the ability of m. smegmatis to multiply in culture. likewise, there appeared to be correspondence between tellurite reduction ... | 1988 | 3179056 |
| effect of in vitro formed immune complexes on macrophage functions of mycobacterium leprae infected mice. | nonspecific macrophage functions were studied in mycobacterium leprae infected and preformed immune complex (ic) administered normal (ni) and thymectomized/irradiated (tri) mice at different time periods. uninfected controls given ic were also included. significant decrease in the chemotaxis, phagocytosis and bactericidal activities of macrophages obtained from infected groups compared to their controls were observed. phagocytic and chemotactic activities of macrophages were normal but intracell ... | 1988 | 3192973 |
| detection of phenolic glycolipid i of mycobacterium leprae and antibodies to the antigen in sera from leprosy patients and their contacts. | 1988 | 3195153 | |
| solubilization of preformed immune complexes in sera of patients with type 1 and type 2 lepra reactions. | serum complement activity in leprosy patients has been studied using solubilization of preformed immune complexes as an index. the solubilization capacity of sera from lepromatous patients with or without erythema nodosum leprosum (enl) as well as from type 1 reactional patients was found markedly reduced as compared to controls. solubilization did not improve at all in the enl patients after remission of the reaction phase. the addition of fresh normal sera failed to bring about any significant ... | 1988 | 3221112 |