Publications

c. parvum treatment of transplanted rat tumours of spontaneous origin.	c. parvum (wellcome cn6134) has been examined for suppression of a range of transplanted rat tumours of spontaneous origin. with five tumours (three mammary carcinomas and two fibrosarcomas) growth of comparatively high cell inocula (with respect to the minimum for growth in control rats) was suppressed by admixture with the vaccine. equivalent dry weights of glaxo, pasteur or connaught bcgs were relatively ineffective. intralesional injection of c. parvum into three three established tumours (t ...	1979	385514
direct effects of corynebacterium parvum and bcg on human monocyte-mediated tumour cell cytostasis in vitro.	four strains of corynebacterium parvum (cp) and bcg induced low levels of cytostatic ability to a human tumour cell line in human monocytes when added directly to conventional monocyte cultures. the cytostatic ability induced by mediators from autologous lymphocytes stimulated with the same agents was greater than that produced by direct addition to monocytes. bcg was more efficient in stimulating lymphocyte dna-synthesis and lymphokine release than any of the cp strains tested. in order to test ...	1979	386712
effects of corynebacterium parvum and bcg therapy on immune parameters in patients with disseminated melanoma. a sequential study over 28 days. ii. changes in non-specific (nk, k and t cell) lymphocytoxicity and delayed hypersensitivity skin reactions.	c. parvum and bcg produced significant changes in nk, k and t cell lymphocytotoxicity using a chang liver target cell. a consistent temporal pattern over 28 days of early depression, recovery, overshoot and then decline was described. this was particularly marked for c. parvum and 'k' cell activity. skin test reactivity to recall antigens at 28 days was not appreciably different from the pre-immunization reactivity. the importance of using lymphocyte concentration-cytotoxicity titration curves a ...	1979	312169
antigenic bacterial polysaccharide in rheumatoid synovial effusions.	phenol-water extracted rheumatoid synovial fluids and synovial fluid leukocytes contain an antigen immunologically identical to the proprionibacterium group bacteria. the antigen was identified by counter-immunoelectrophoresis in 70% of rheumatoid synovial fluid leukocyte pellets and in 60% of rheumatoid synovial fluids. it was also present in 6% of nonrheumatoid fluids and in 22% of nonrheumatoid inflammatory fluid leukocytes. antigen was not detectable in synovial samples before extraction. sy ...	1979	314293
the effect of bcg-vaccination on vaccinia virus infections in mice.	pretreatment with bcg yielded a high degree of protection against experimental vaccinia virus infections in mice. corynebacterium parvum and aristolochia acid were less protective; other immunostimulants were ineffective.	1979	389657
the effect of acidic polysaccharides and prostaglandin-like substances isolated from propionibacterium acnes on granulocyte chemotaxis.	three acidic polysaccharide (ap) fractions and the prostaglandin-like substances (pls) isolated from p. acnes were investigated regarding their chemotactic activities on polymorphonuclear leukocytes. both ap's and pls induced a significant chemotatic response, which suggests their involvement in inflammatory acne vulgaris.	1979	391585
effects of repeated corynebacterium parvum and bcg therapy on immune parameters: a weekly sequential study of melanoma patients. i. changes in non-specific (nk, k and t cell) lymphocytotoxicity, peripheral blood counts and delayed hypersensitivity reactions.	increased lymphocyte cytotoxicity, particularly of 'killer' k cell type was recorded with repeated immunizations of either c. parvum or bcg. a 3 week interval between immunizations was capable of maintaining the increase in cytotoxicity. no marked alterations of 'recall' skin hypersensitivity reactions nor of peripheral blood counts were noted. expression of cytotoxicity results as percentage 51cr release, lytic units/ml and cytotoxic capacity (after logit transformation of the cytotoxicity-lymp ...	1979	314367
value of immune monitoring in gynecologic cancer patients receiving immunotherapy.	immunotherapy with nonspecific immunopotentiators such as corynebacterium parvum is under study in several areas of gynecologic oncology. the efficacy of this new modality awaits the results of carefully done clinical trials designed to measure an improved effect on survival when this and similar agents are added to conventional therapy. this report deals with an attempt to demonstrate enhancement of the cell-mediated immune response in a small number of patients with advanced ovarian (18 patien ...	1979	315714
antitumor activity of cell-wall skeleton of propionibacterium acnes c7 in mice and guinea pigs.	the antitumor activity of the cell-wall skeleton (cws) of propionibacterium acnes c7 was examined by using transplantable tumors in syngeneic mice and in guinea pigs, and autochthonous tumors in mice. p. acnes-cws was shown to suppress the growth of fibrosarcomas, el4 leukemia, and mh134 hepatoma in syngeneic mice, and to regress the established tumors of a fibrosarcoma (mc104) in c57bl/6j mice, and a hepatoma (line-10) in strain-2 guinea pigs. the oil-attached p. acnes-cws mixed with fructose m ...	1979	395008
human monocyte-mediated cytotoxicity to k-562 cells: activation by lymphokines.	human monocytes purified by adherence and prolonged in vitro monolayer culture were activated by supernatants of autologous lymphocytes stimulated with live bacillus calmette-guérin or killed corynebacterium parvum. activated monocytes expressed increased ability to lyse k-562 cells prelabelled with methyl-3h-thymidine in a 48 h assay. activation could be detected at a 1:64 dilution of lymphokine supernatants. target cell killing by activated monocytes was strongly influenced by the density of t ...	1979	396667
influence of tumor burden, tumor removal, immune stimulation, plasmapheresis on monocyte mobilization in cancer patients.		1979	397757
inhibition of host resistance by nutritional hypercholesteremia.	previous experiments showed that nutritionally induced hypercholesteremia in mice caused an increase in susceptibility to coxsackievirus b, with a marked suppression of cellular infiltrates in infected tissues and an increased mortality. the present studies demonstrated that a hypercholesteremic diet was associated with an inhibition in host resistance as measured by susceptibility to listeria monocytogenes infection and the growth of two transplanted syngeneic murine tumors. moreover, the abili ...	1979	317596
phagosome/lysosome fusion: a possible prerequisite for the enhancement of antibody responses in vitro by bcg, mycobacterium leprae and corynebacterium parvum.	primary in vitro antibody responses to srbc were suppressed in cultures prepared from the spleens of cba mice injected i.v. 20 days previously with 10(8) liver bcg. in contrast, cultures prepared from mice injected with dead bcg showed enhanced responses. in vitro spleen cell responses of the mice had returned to normal levels 4--6 weeks after their injection, but if dead bcg, m. leprae or c. parvum was added to the cultures, responses were enhanced. the enhancing effect of the added bacteria co ...	1979	399339
response of lympho-hemopoietic tissue to corynebacterium parvum by study of dna enzymes and h3-tdr metabolism.	the effects of corynebacterium parvum on the lymphohemopoietic tissues of mice was investigated by h3-tdr metabolism, organ weights, dna-polymerase-alpha activity and terminal deoxynucleotidyl transferase (tdt) activity. marked increase in spleen size occurred. the increase in size was accompanied by increases in dna-p-alpha activity and h3tdr uptake. this indicated that the spleen was a major site of cell proliferation and of increase in population size after c. parvum stimulation. thymus and b ...	1979	317898
cocarcinogenesis.		1979	367444
[current status of immunotherapy of malignant tumors].		1979	367730
immunotherapy of solid tumor.		1979	375448
defective tumoricidal capacity of macrophages from a/j mice. i. characterization of the macrophage cytotoxic defect after in vivo and in vitro activation stimuli.	macrophages from a/j mice fail to develop tumoricidal activity after any of several in vivo or in vitro treatments that activate cells from c3h/hen mice. peritoneal macrophages from a/j mice treated i.p. with viable mycobacterium bovis, strain bcg, killed corynebacterium parvum, or pyran copolymer fail to develop in vitro tumoricidal activity; varying the numbers of macrophages from treated mice added to target cells, or the dose and time of treatment, or the treatment schedule of these in vivo ...	1979	376720
in vitro killing of schistosomula of schistosoma mansoni by bcg and c. parvum-activated macrophages.	resistance to schistosoma mansoni infection in the mouse has been induced either specifically by a primary infection with this parasite or nonspecifically by a variety of immunostimulants such as bcg. in the present study we developed an in vitro system to examine the effector mechanism of nonspecifically induced resistance. activated macrophage monolayers obtained from bcg- or corynebacterium parvum treated mice killed a respective mean 32 +/- 6% and 48 +/- 5% of schistosomula after 24 hr incub ...	1979	376723
current state of clinical immunotherapy.		1979	377611
tumor growth inhibition and potentiation of immunotherapy by indomethacin in mice.	indomethacin was continuously administered in the drinking water of inbred c3h mice given grafts of syngeneic 3-methylcholanthrene-induced fibrosarcomas. a minor proportion of these animals died at the same time as the untreated controls, and others completely rejected their tumors; however, in most cases, the tumor growth rate was significantly slowed, and growth recommenced rapidly after drug withdrawal. this was the pattern for tumors either in their 10th to 14th transplant generation or only ...	1979	281566
cyclophosphamide plus 5-(3,3-dimethyl-1-triazeno)-imidazole-4-carboxamide (dtic) with or without corynebacterium parvum in metastatic malignant melanoma.	one hundred twenty patients with metastatic malignant melanoma were randomized to receive either cyclophosphamide, 600 mg/m2 iv, on day 1 plus dtic 200 mg/m2 iv days 1 through 5, or the same chemotherapy plus c. parvum 5 mg/m2 iv on day 8 and day 15. therapy was repeated every 21 days. although responses were observed in 13.8% of patients on cyclophosphamide plus dtic versus 25.5% of patients on cyclophosphamide plus dtic plus c. parvum, the median duration of remission was 15.6 weeks on chemoth ...	1979	383276
the aerobic growth of propionibacterium acnes in primary cultures from skin.	although propionibacterium acnes ordinarily grows as an anaerobe, this organism was commonly seen on aerobic primary cultures (directly from skin) of the forehead, axilla, deltoid area, and antecubital fossa. on subculture, strains from aerobic primary cultures showed their normal anaerobic state. on repeated tests of the foreheads of 7 subjects over periods of 1 to 4 yr, 24% of all cultures were positive for propionibacteria (p. acnes) on aerobic culture. this phenomenon was equally common in c ...	1979	422866
conditions for effective bacillus calmette-guérin immunotherapy of postsurgical metastases of 13762a rat mammary adenocarcinoma.	we evaluated critical variables in bacillus calmette-guérin (bcg) immunotherapy of residual 13762a rat mammary adenocarcinoma. bcg was given intratumorally on day 7 of tumor growth and followed by primary tumor excisions on day 20. untreated animals died on about day 40 with axillary nodal and pulmonary parenchymal metastases. bcg-treated animals experienced prolonged survival, and some were cured. the highest dose (5.0 x 10(7) colony-forming units) of bcg was more effective than the lowest (0.5 ...	1979	427785
prostaglandin-like substances in propionibacterium acnes. iii. differential contractile effects on smooth muscle layers of the human utero-tubal junction.	the biological activity of a lipid fraction extracted from p. acnes was tested on isolated smooth muscle strips from the human utero-tubal junction. the bioassay experiments support the concept that prostaglandin-like substances (pls) occur in p. acnes. however, in the bioassay system used, the effect of pls was different from that of pgf2 alpha and pgi2 but similar, although not identical, to that of arachidonic acid and pge2.	1979	387431
fatal infection with pseudomonas aeruginosa in mice treated with cyclophosphamide and propionibacterium acnes.		1979	428451
[protection of mice with bacterial phospholipids against the lethal effect of frog virus 3 (fv 3) (author's transl)].	a bacterial phospholipid extract (ebp) inoculated intraveinously at a dose of 1 mg/25 g body weight 30 hours before infection protects mice against the lethal effect of frog virus 3 (fv 3). the anti-fv 3 resistance produced by ebp requires protein synthesis during the period of pretreatment. the treatment with the bacterial extract has no effect on the inhibition of the macromolecular synthesis of the liver (rna and dna) which is observed at the beginning of the infection. however 48 hours after ...	1979	388298
aerobic and anaerobic bacterial flora of the maternal cervix and newborn gastric fluid and conjunctiva: a prospective study.	aerobic and anaerobic cultures were performed on cervical swabs from 35 mothers and their newborn infants' gastric aspirates and conjunctival sacs. four hundred seventy-nine isolates were obtained; 287 were aerobes and 192 were anaerobes. the cervical and gastric cultures overall yielded a similar flora, but this was not always the case when one compared mothers with their own newborn infants. the conjunctival cultures yielded about half of the number of bacteria per specimen that the gastric co ...	1979	440845
chemoimmunotherapy (dtic and corynebacterium parvum) as adjuvant treatment in malignant melanoma.	sixty-one patients with stage i or ii (lymph node involvement) malignant melanoma were prospectively randomized into a control group receiving surgical treatment only (consisting of wide excision of primary and regional node dissection) and a group receiving the same surgical treatment plus adjuvant therapy with dtic and corynebacterium parvum. followup times ranged from 1 1/2 to 4 years. among the 29 patients in the surgical control group, there were five hematogenous recurrences and one region ...	1979	393377
immunotherapy of lung cancer. i. review of clinical trials in non-small cell histologic types.		1979	394836
metabolic changes following the intravenous infusion of corynebacterium parvum in man.	the acute changes in concentrations of key blood metabolites and liver function tests were measured following intravenous infusion of corynebacterium parvum in 9 healthy patients who had recently undergone resection of a colorectal cancer. the following results were obtained: 1) blood glucose, lactate and ketone body concentrations significantly increased over a 5 hour study period; 2) blood alanine fell during the same period; 3) plasma bilirubin, got and urea were significantly elevated 24 hou ...	1979	445333
immunotherapeutic response of concanavalin a-bound l1210 vaccine enhanced by a streptococcal immunopotentiator, ok-432.	immunotherapeutic response to concanavalin a (con a)-bound l1210 murine leukemic vaccine and immunopotentiators was examined in histocompatible animals bearing a small burden l1210 leukemic cells. when combined with con a-bound vaccine, a streptococcal immunopotentiator, ok-432 (nsc b116209), prepared from streptococcus pyogenes, was potent in antitumor therapy and resulted in a number of cured animals. administration of either con-a-bound vaccine or ok-432 alone did not produce any beneficial e ...	1979	445486
immune response modulation by colonization of germfree rats with propionibacterium acnes.	propionibacterium acnes (oral and/or parenteral administration) had a modulating effect on antibody-and cell-mediated immune responses of germfree (gf) and monoassociated (ma) rats. in conventionally reared rodents, parenteral injection of killed p. acnes stimulated the splenic plaque-forming cell response to sheep erythrocytes. however, in gf rats and in rats monoassociated with viable p. acnes, parenteral injection of killed p. acnes antigen inhibited the plaque-forming cell response to sheep ...	1979	397928
further observations on the inhibition of tumor growth by c. parvum with cyclophosphamide. vii. effect of treatment prior to primary tumor removal on the growth of distant tumor.	the present investigations were directed toward determining whether primary tumor manipulation prior to its removal is advantageous for the control of metastases and survival. studies were carried out to ascertain whether 1) there is justification for delaying surgical removal of a primary tumor to permit preoperative administration of cyclophosphamide (cy) and/or c. parvum (cp) and 2) there is an advantage to administering the immunotherapy directly into a primary tumor. after operation, in all ...	1979	421172
effect of corynebacterium parvum on liver proliferation and regeneration.		1979	421219
enhancement of the thermal response of animal tumors by corynebacterium parvum.	the effect of corynecbacterium parvum treatment on the thermal response of animal tumors was studied. tumors were methylcholanthrene-induced (fsa-ii) and spontaneous (fsa-i) fibrosarcomas in c3hf/sed mice. c. parvum was given i.v. and was followed by local hyperthermia at 43.5 degrees 3 days later. cell survival determined by lung colony assays showed that preadministration of c. parvum insignificantly enhanced the thermal response of both tumors. studies of delay of tumor growth for fsa-ii demo ...	1979	476675
[effect of corynebacterium parvum on serum lysozyme (muramidase) levels (author's transl)].	an i.v. injection of 548 microgram of killed corynebacterium parvum into c57b1 mice leads to significant changes in serum lysozyme (muramidase) levels. after an initial fall at 24 h, the activity of the enzyme increased progressively, reached a peak on the 9th day and returned to control range after the 15th day.	1979	477851
laboratory induction and clinical occurrence of combined clindamycin and erythromycin resistance in corynebacterium acnes.	corynebacterium acnes strains cross-resistant to clindamycin and erythromycin were observed following long-term selection or mutagenic treatment in the laboratory. similar strains were found among clinical isolates from patients using clindamycin or erythromycin topically in the treatment of acne vulgaris. clindamycin resistance was never observed in the absence of resistance to macrolides or other lincosaminides. it is suggested that this resistance may result from an alteration of the 50s ribo ...	1979	429800
[porphyrin production of propionibacterium acnes].		1979	442749
lack of effect of immunotherapy with bcg and corynebacterium parvum on hepatic drug hydroxylation in man.	serial serum diphenylhydantoin and urinary 5-(p-hydroxphenyl)-5-phenylhydantoin concentrations were determined in 8 patients with malignant disease and 4 healthy volunteers on 2 separate occasions after an oral dose of diphenylhydantoin (500 mg). no significant difference was observed between metabolism before and 10 days after immunization with bcg or corynebacterium parvum. volunteers without intervening immunization similarly showed no difference.	1979	444399
chemoimmunotherapy in the management of primary stage iii ovarian cancer: a gynecologic oncology group study.	the effectiveness of chemoimmunotherapy was evaluated in 45 previously untreated stage iii ovarian cancer patients. response rate, progression-free interval, and survival were considerably better than in a similar group of 63 patients treated with a single alkylating agent alone.	1979	445505
effect of 1(4-amino-2-methyl-5-pyrimidinyl) methyl-3-(2-chloroethyl)-3-nitrosourea hydrochloride (acnu) on sato lung carcinoma (slc). preliminary result of immunochemotherapy for slc by combination of acnu and propionibacterium acnes.	acnu was highly effective for sato lung carcinoma transplanted intravenously or intramuscularly by using a large single dose, and the cytotoxic action of acnu for slc showed clear dependence upon tumor size. nonspecific activation of host-defence mechanism by propionibacterium acnes contributed to the suppression for the regrowth of solid slc treated by acnu.	1979	450390
anaerobic bacteria in late infections following orthopedic surgery.	twelve patients with late anaerobic infection following hip or knee arthroplasty or tibia osteosynthesis are presented. peptococci (peptococcus asaccharolyticus, p. magnus, p. prevotii) and peptostreptococci (peptostreptococcus micros, p. anaerobius, p. parvulus) were present in nine of the cases. anaerobic gram-positive or -negative rods were found in three cases (propionibacterium acnes, actinomyces israelii, and bacteroides fragilis). antibodies against the isolated bacteria were detected in ...	1979	492071
pharmacokinetics of phenytoin and liver cytochrome p-450 levels in mice after single doses of corynebacterium parvum [proceedings].		1979	497584
comparative antitumor effects of corynebacterium parvum, bordetella pertussis, bacillus calmette-guérin, and levamisole alone or in combination with cyclophosphamide in the cad2 murine mammary adenocarcinoma system.	the antitumor efficacy of various immune stimulants [corynebacterium parvum, bordetella pertussis, and bacillus calmette-guérin (bcg)] and levamisole alone or in conjunction with cyclophosphamide (cy) was studied in the cad2 mammary adenocarcinoma system using schedules developed previously with c. parvum and cy. weekly systemic treatment with c. parvum, b. pertussis, or bcg was effective in controlling tumor growth and had equivalent antitumor effects, but weekly treatment (or a single treatmen ...	1979	498111
propionibacterium acnes pneumonia in a patient with lymphoma.	a 57 year-old male with lymphocytic lymphoma developed symptoms of cough, fever and progressive dyspnea accompanied by a diffuse lower lung infiltrate. open lung biopsy revealed multiple micro-abscesses surrounding small bronchioles which yielded a pure growth of propionibacterium acnes.	1979	478654
positive correlation between the levels of natural killer cells and the in vivo resistance to syngeneic tumor transplants as influenced by various routes of administration of corynebacterium parvum bacteria.		1979	455483
lung cancer patients treated with corynebacterium parvum. phase i toxicity study.		1979	503003
macrophage activation and increased resistance to infection in immunosuppressed mice treated with corynebacterium parvum vaccine or pyran copolymer.		1979	458782
quantitative and functional differences induced in bone marrow-derived mononuclear phagocytes by inflammatory stimuli.		1979	458783
comparison of the in-vitro antitumor activity of peritoneal cells from mice injected with corynebacterium parvum and corynebacterium liquefaciens.		1979	478988
dual effect of normal and stimulated macrophages and their conditioned media on target cell proliferation.		1979	480375
activation of human monocytes by mediators from lymphocytes stimulated with corynebacterium parvum.	human monocytes activated in vitro by lymphokine-containing supernatants of autologous or allogeneic lymphocytes stimulated in vitro by corynebacterium parvum (cp) expressed increased ability to suppress dna-synthesis in a human tumour cell line. monocyte activation was not dependent on in vitro differentiation of monocytes, enhanced cytostatic ability being observed at all stages of in vitro differentiation. the lymphokine-induced cytostatic ability was not affected by intensive washing and try ...	1979	484237
[prospects of short-term sequential immunomodulation with levamisole and corynebacterium parvum in neoplastic pathology in the aged].		1979	460632
[postoperative prevention of recurrence with intrapleural corynebacterium parvum in patients with stage i and ii bronchial carcinomas. ludwig lung cancer study i].	the ludwig lung cancer group was created in 1977. participants are from austria, denmark, germany, norway, sweden, switzerland, and yugoslavia. 400 patients are randomized yearly. the clinical trial i investigating the role of c. parvum intrapleurally as adjuvant therapy in operable non-small cell lung cancer patients has been closed in february 1979 with a total accrual of 475 patients. the average follow-up time for these cases is approximately 8 months. it is early to make any definitive comp ...	1979	462160
effect of corynebacterium parvum in prevention and reversal of atrophy of the liver following portacaval shunt.	since it has been demonstrated that the administration of corynebacterium parvum is associated with increased hepatocyte proliferation in both normal and regenerating livers, it seemed appropriate to determine whether the use of this agent would affect the hepatic atrophy which occurs following end-to-side portacaval shunts. when administered at shunting, liver weight and liver deoxyribonucleic acid failed to decrease to the same extent as that occurring in untreated, shunted rats. an increase i ...	1979	462347
investigations on the bacteriostatic effect of benzoyl peroxide.		1979	464656
is there postdefecation bacteremia?	an investigation was done to determine the incidence of postdefecation bacteremia. a study of 82 healthy volunteers was done. among 164 predefecation cultures, only one (0.6%) from an anaerobic flask was positive for microorganism, which was identified as staphylococcus epidermidis. in only two of 328 (0.6%) postdefecation cultures did microorganisms grow, which were shown to be propionibacterium acnes. the results of this study suggest that either bacteremia after defecation does not occur or i ...	1979	464809
growth of cutaneous propionibacteria on synthetic medium; growth yields and exoenzyme production.		1979	541303
prolonged effect of corynebacterium parvum stimulation on granulopoiesis.	an intravenous injection of 548 microgram of killed corynebacterium parvum (c. parvum) into c57bl mice led to a significant increase in the number of both committed stem cells (gm-cfc) and proliferating cells of bone marrow granulocytic series. this increase appeared 24 h after the injection of the bacterium, persisted in varying levels until the 4th week and was accompanied by a marked and prolonged granulocytosis. the proportion of gm-cfc in dna synthesis was found to be increased 1, 2, and 5 ...	1979	542797
comparison of three methods of susceptibility testing of bacteroidaceae, peptococcaceae and other anaerobes to doxycycline.	the in vitro activity of doxycycline against 147 strains of gram-positive and gram-negative anaerobes was determined by broth dilution, agar dilution and agar diffusion tests. the strains were isolated from clinical specimens in 1977. saccharolytic bacteroides strains (39 b. fragilis, 6 b. thetaiotaomicron, 4 b. vulgatus) showed broth dilution mics of less than or equal to 0.0625-4.0 microgram/ml after 5 h incubation and mics of less than or equal to 16 microgram/ml after 15 h incubation at 37 d ...	1979	543353
regression of regionally advanced melanoma after arterial infusion with cis-platinum and actinomycin-d.		1979	466896
choriogonadotropin-like antigen in an anaerobic bacterium, eubacterium lentum, isolated from a rectal tumor.	using the indirect fluorescein-labeled and indirect peroxidase-antiperoxidase-labeled immunohistochemical techniques, and utilizing both antiserum specific for the beta-subunit of choriogonadotropin and antiserum for the total hormone, we have demonstrated the presence of a choriogonadotropin-like immunoreactive material in a strain of eubacterium lenthum that was originally isolated from a rectal tumor. in contrast, both immunohistochemical reactions were negative when applied to a strain of co ...	1979	468380
[equal sensitivity of normal or tumoral fibroblasts to cytotoxic and cytostatic effects of normal or "corynebacterium parvum" activated peritoneal exudate cells (author's transl)].	cytotoxic and cytostatic properties of peritoneal exudate cells from balb/c mice either normal or pretreated with corynebacterium parvum normal were studied using as target cells two lines of balb/c fibroblasts one normal (balb) and one transformed by kirsten virus (k.balb).. c. parvum activated cells displayed higher cytotoxic as measured by 51cr or 3h-thymidine release and cytostatic, as measured by 3h-thymidine incorporation, activities against these lines of syngeneic cells. cytotoxic effect ...	1979	475346
an ultrastructural study of peritoneal mononuclear phagocytes from corynebacterium parvum-injected mice.	peritoneal mononuclear phagocytes harvested from mice 24 h after i.p. injection of c. parvum displayed hypertrophy of the golgi and smooth endoplasmic reticulum complex with attendant increase in lysosome production. the ingested bacilli were identified within phagolysosomes, within which large myelin figures accumulated. in addition to heterophagic vacuoles, autophagosomes and lipid droplets were observed. these latter two inclusions may reflect a direct or indirect cytopathic effect of c. parv ...	1979	475974
inhibition of spontaneous akr leukemia by multiple inoculations of corynebacterium parvum.	in previous studies we have shown that akr mice could be protected from spontaneous leukemia by specific immunotherapy in combination with splenectomy. in this experiment we investigated the effects of nonspecific immunotherapy with c. parvum in a similar regimen. it was found that bi-weekly, ip inoculations of 0.7 mg of c. parvum could significantly protect akr mice from spontaneous tumors, and that splenectomy could not modify this effect. thus, the spleen does not appear to play a determinant ...	1979	476565
effect of adriamycin and corynebacterium parvum in tumor-bearing mice: modulation of response to sheep red blood cells.	administration of adriamycin and corynebacterium parvum alone in c57bl/6j mice bearing lewis lung carcinoma stimulated the direct (19s) and indirect (7s) plaque-forming cell (pfc) response specific for sheep red blood cells. thus adriamycin appears to possess some immunostimulatory effect on tumor-bearing mice with much less effect than c. parvum alone. simultaneous administration of adriamycin and c. parvum decreased the pfc response compared to that for c. parvum alone. this decrease may indic ...	1979	379400
corynebacterium parvum: effects on the biochemistry of mouse serum and liver.		1979	379402
effects of corynebacterium parvum and bcg therapy on immune parameters in patients with disseminated melanoma a sequential study over 28 days. i. changes in blood counts, serum immunoglobulins and lymphoid cell populations.	the effects of a single immunization of melanoma patients with bcg or c. parvum on the blood counts, serum immunoglobulin levels and lymphoid subpopulations were followed by multiple assays over 28 days. c. parvum produced a decrease in the white cell count, lymphocyte count and lymphoid t and sig+ cell numbers, which recovered within 1 week; bcg did not produce such a marked depression. both agents were associated with increases in t cell numbers and lymphocyte pha blastogenesis after the first ...	1979	428146
sensitivity to chemotherapeutic and immunomodulating agents of two mouse lymphomas and of a macrophage tumor.	the effect of treatment with 15 chemotherapeutic and 10 immunomodulating agents on the growth of t-cell lymphoma el-4, macrophage tumor j774, and b-cell lymphoma 70z/2 of the mouse has been studied using the prolongation of median survival time of tumor-bearing hosts as an index of therapeutic effectiveness. the survival time of mice bearing 70z/2 was prolonged more than 100% by single-agent therapy with actinomycin d, cyclophosphamide, 6-mercaptopurine, mitomycin c, and vinblastine; a similar r ...	1979	476634
monocytes and macrophages in malignant melanoma iv. effects of c. parvum on monocyte function.	assays for the capacity of peripheral-blood monocytes (a) to mature in vitro into macrophages, (b) to reduce nitro-blue tetrazolium (nbt) and (c) to lyse antibody-coated human group a red cells, were applied to a group of 82 patients with histologically proven malignant melanoma. in patients with micrometastatic disease there was an enhancement of red-cell lysis and nbt reduction, suggesting that their monocytes are in some way "activated", whereas nbt reduction was suppressed in those with over ...	1979	486312
fc receptor-bearing and phagocytic cells in syngeneic tumours of c. parvum- and carrageenan-treated mice.		1979	486317
effects of repeated corynebacterium parvum and bcg therapy on immune parameters: a weekly study of melanoma patients ii. changes in serum immunoglobulins and lymphoid cell subpopulations.	two groups, each of eight patients, with metastatic melanoma were immunized with either c. parvum or bcg at 3-weekly intervals. the serum igg, iga, igm levels, e-rosettes, ea-rosetts, sig-bearing cells, 'null cells' and lymphocyte blastogenesis at four pha concentrations were assayed, before immunization and weekly thereafter for 8 weeks. the pre-immunization e-rosette values were significantly reduced, with a significant increase in 'null cell' values and ea-rosette percentages, when compared w ...	1979	487647
effects of whole body irradiation in mice treated with c. parvum.		1979	499277
host treatments affecting artificial pulmonary metastases: interpretation of loss of radioactively labelled cells from lungs.	the effect has been examined of various host treatments (c. parvum injection, immunization, thoracic irradiation, cyclophosphamide injection, and anticoagulation) on both lung colony formation and clearance of radioactive cells from the lungs after i.v. injection of tumour cells. two tumour-host models have been used: the non-immunogenic kht tumour in c3h/km mice, and the immunogenic emt6 tumour in balb/c/ka mice. even for the at most weakly immunogenic kht tumour, the number of artificial pulmo ...	1979	508577
[adriamycin-combination chemotherapy with or without immune stimulation by corynebacterium parvum in metastasizing carcinoma of the breast (author's transl)].	in a randomised, multicentre study the effect of changing the combined cytostatic treatment with vac (vincristin, adriamycin, cyclophosphamide) to fmc (fluorouracil, methotrexate, cyclophosphamide) was tested, as well as the effect of active non-specific immune stimulation with corynebacterium parvum, and compared with a control group. in 59 of 120 patients (49%) in whom the results could be analysed there was a measurable significant regression of tumour size to less than 50% of its initial val ...	1979	510206
macrophage precursors in ovarian carcinoma.		1979	510998
alterations induced in macrophage and granulocyte-macrophage colony-forming cells by a single injection of mice with corynebacterium parvum.		1979	513059
chemical and biological properties of a purified lymphoreticular-stimulating fraction of corynebacterium parvum (propionibacterium acnes type i).	a method is described in which washed whole cells of corynebacterium parvum were chemically and enzymatically extracted to remove cytoplasm and cell-wall lipids. the resultant insoluble cell-wall residue possessed lympho-reticular stimulating properties as measured by their ability to increase spleen weight and protect against tumour-cell challenge. analysis of the final product by chromatography and infrared spectroscopy has shown it to consist of carbohydrate and peptidoglycan, both of which a ...	1979	513108
non-specific and specific active immunotherapy in a b16 murine melanoma system.	conflicts amongst reports concerning the efficacy of both nonspecific and specific attempts at immunotherapy may be ascribed to different animal models utilizing tumors of different immunogenicity. we have selected the b16 mouse melanoma model as the example of a spontaneously occurring neoplasm that is histocompatible with the host and does have tumor-associated antigens. attempts to alter tumor growth or survival with nonspecific active immunotherapy as well as with specific active immunothera ...	1979	480953
the facilitation of tumour growth in the lung by cyclophosphamide in artificial and spontaneous metastases models.		1979	527634
action of cancer chemotherapy-radiotherapy on spleen hypertrophy response to corynebacterium parvum adjuvant therapy.		1979	536279
biotyping of propionibacterium acnes isolated from normal human facial skin.	biochemical and serological characteristics of 128 strains of propionibacterium acnes isolated from the facial skin of healthy japanese volunteers were compared with the three standard strains of the american type culture collection, atcc 6919, 11827, and 11828. accordingly, the isolated strains of p. acnes were classified into five biotypes (b1 to b5) on the basis of fermentation tests of ribose, erythritol, and sorbitol. two serotypes were distinguished by the agglutination test. p. acnes belo ...	1979	539817
effects of intravenous injection of two different strains of corynebacterium parvum in the mouse.	a strain of c. parvum, cn6134, known to have antitumour activity, caused thrombosis in the sites where the organism is phagocytosed. it bound to macrophages in vitro and activated the alternate pathway of complement. a strain of c. parvum, cn5888, which fails to show antitumour activity, did not show thrombosis. it did not bind to macrophages or activate guinea-pig complement. it did, however, cause marrow infarction which seems to result from a diminished clearance of the organism from the circ ...	1979	540104
protection of mice against endotoxin-induced liver damage by anti-inflammatory drugs.	mice were injected with corynebacterium parvum, which induces multiple granulomas in liver and renders animals hyper-reactive to the lethal effect of bacterial lipopolysaccharide (lps). such animals when challenged with lps developed also extensive liver parenchymal cell damage as estimated by elevated blood asparate transaminase levels and a hypoglycaemia. treatment with indomethacin, hydrocortisone, dexamethasone, promethazine, metiazinic acid and (+)-catechin ameliorated the liver damage. hyd ...	1979	546179
activation of pleural macrophages by intrapleural application of corynebacterium parvum.	a single ipl injection of 0.25 mg cp into cba mice led to accumulation of macrophages in the pleural cavity, but it did not influence res as an injection given iv ipl cp caused a three-to-five-fold increase in the number of nucleated cells in the pleural cavity which persisted at least 14 days. of these cells 86% were macrophages as shown by their esterase activity. less than 30% of cells from the pleural cavity of normal mice were esterase positive. macrophages from the pleural cavity of cp-tre ...	1979	547729
role of mononuclear phagocytes in decreased hepatic drug metabolism following administration of corynebacterium parvum.		1979	551956
[in vitro sensitivity of corynebacterium acnes (propionibacterium acnes) to meclocycline, clindamycin, erythromycin and neomycin].		1979	554810
active, specific immunotherapy of murine leukemia. ii. adjuvant effect of corynebacterium parvum.	we have previously shown that irradiated lstra cells (lx) were immunogenic and could prolong survival of mice bearing lstra tumors. this study demonstrated that addition of corynebacterium parvum to the lx dramatically improved the strength of tumor immunity. in pretreatment-challenge experiments, c. parvum augmented the immunogenicity of 10(6) lx given intradermally, with an optimum dose of 0.14-1.4 microgram c. parvum per mouse. in therapy experiments (intraperitoneal vaccine treatment after t ...	1979	528079
the reversal of surgically induced reticuloendothelial depression.	the function of the reticuloendothelial (re) system may be depressed by surgical operation. as this system is an important defence mechanism against tumour growth and metastasis, it would be an advantage if this depression could be prevented by the administration of a suitable agent. the agents studied in this case were levamisole, corynebacterium parvum (c. parvum) and glucan. as a measure of re function a carbon clearance test was performed in rats, 1 hr after partial colectomy, animals having ...	1979	487654
immunotherapy of a murine ovarian carcinoma with corynebacterium parvum and specific heteroantiserum. i. activation of peritoneal cells to mediate antibody-dependent cytotoxicity.	immunotherapy with a combination of corynebacterium parvum and specific heteroantiserum is significantly more effective than treatment with either single agent in prolonging the survival of mice that have recevied an i.p. injection of syngeneic murine ovarian carcinoma (mot) cells. invitro, a combination of c. parvum-activated peritoneal cells and specific heteroantiserum has proven significantly more effective than either single component in destroying 51cr-labeled mot cells in the absence of c ...	1979	489967
chemoimmunotherapy for meningeal melanocytoma of the thoracic spinal cord. report of a case.		1979	490860
surgery plus corynebacterium parvum immunotherapy for lewis lung carcinoma in mice.		1979	499275
effect of corynebacterium parvum on hemopoietic stem-cell kinetics.	the intravenous administration of 548 microgram of killed corynebacterium parvum (c. parvum) into c57bl mice leads to a significant decrease in the number of bone-marrow colony-forming-units in spleen (cfus) as early as 12 h after the injection of the bacterium. this decrease persisted in varying degrees for 3 weeks. after an initial fall at 24 h, the splenic cfus exhibited a rapid expansion and reached values 10 times higher than the control range on the ninth day. a significant rise in the num ...	1979	759377
selective antibacterial action of 2-mercaptoethanol on propionibacteria in skin cultures.	2-mercaptoethanol applied to the surface of agar medium had a selective antibacterial effect on propionibacterium acnes and propionibacterium granulosum without interfering with the growth of peptococcus saccharolyticus or staphylococci in anaerobic cultures of skin or in pure cultures. in aerobic broth culture, 2-mercaptoethanol inhibited aerobes and stimulated anaerobes, consistent with its action as a reducing agent.	1979	760636
effects of dose and schedule of immune stimulant on efficacy of combination corynebacterium parvum-cyclophosphamide treatment for a murine mammary adenocarcinoma.	certain variables which might influence the outcome of combining cytotoxic drug and immune stimulant therapy were studied to optimize the effectiveness of corynebacterium parvum combined with cyclophosphamide (cy) as treatment for a murine mammary adenocarcinoma (cad2). optimal effects of combined c. parvum-cy treatment in the cad2 system were obtained when 443 to 1400 microgram of this immune stimulant per mouse were injected 2 to 3 days after cy chemotherapy and when combination treatment was ...	1979	761179
purification and properties of a bacteriocin-like substance (acnecin) of oral propionibacterium acnes.	propionibacterium acnes cn-8, isolated from human dental plaque, was grown in a liquid medium, and its bacteriocin-like substance (acnecin) was extracted from the cells by ultrasonic treatment. acnecin was purified to a homogeneous state with recovery of 47%. specific activity increased 72-fold in comparison with the crude extract. the properties of acnecin were as follows. (i) acnecin may consist of five subunits with a molecular weight of about 12,000. (ii) its isoelectric point was 5.5. (iii) ...	1978	742876
propionibacteria as a cause of shunt and postneurosurgical infections.	propionibacterium acnes was isolated from postoperative specimens from nine neurosurgical patients. the role of p. acnes in postneurosurgical infection is discussed.	1978	739055
a preliminary study of intralesional, intralymph node, intravenous and intraperitoneal corynebacterium parvum treatments in patients with advanced cancer.	twenty-one patients with squamous cell carcinoma of oral cavity and/or cervical lymph node and four patients with soft tissue sarcomas were given intralesional (2-4 mg/m2) and intralymph node (2-4 mg/m2) c. parvum injections followed by 5 intravenous (2-4 mg/m2) c. parvum treatments and conventional therapies. seven patients with stage iii ovarian carcinomas were given intraperitoneal (2-4 mg/m2) c. parvum injections followed by conventional therapies. the local and systemic effects of the c. pa ...	1978	709538
bacteriological investigation on biopsy specimens from patients with sarcoidosis.	biopsy specimens, mostly lymph nodes, removed under aseptic conditions, were emulsified and the homogenate was inoculated in various media, which were incubated aerobically or anaerobically. only propionibacterium acnes was isolated in high incidence and the highest concentration of bacteria was 6.5 x 10(5) per g tissue: 28 and 31 out of 40 lymph nodes were positive on solid and on liquid media, respectively. p.acnes was also isolated from control non-sarcoidosis lymph nodes: 4 and 8 out of 14 w ...	1978	713130