Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| cloning and partial characterization of zwittermicin a resistance gene cluster from bacillus thuringiensis subsp. kurstaki strain hd1. | the study seeks to shed light on the aminopolyol, broad-spectrum antibiotic zwittermicin a gene cluster of bacillus thuringiensis subsp. kurstaki hd1 and to identify any new uncharacterized genes with an eventual goal to establish a better understanding of the resistance gene cluster. | 2004 | 15281929 |
| three cases of post-traumatic endophthalmitis caused by unusual bacteria. | three cases of post-traumatic endophthalmitis caused by unusual bacteria are presented. the pathogens identified were: (i) bacillus cereus and citrobacter freundii; (ii) pseudomonas fluorescens; and (iii) chryseobacterium meningosepticum and stenotrophomonas maltophilia. two of these pathogens have not previously been reported to cause endophthalmitis. the available literature regarding the individual cases is summarized and a brief discussion of post-traumatic endophthalmitis is presented, with ... | 2004 | 15281988 |
| use of a fluorescent viability stain to assess lethal and sublethal injury in food-borne bacteria exposed to high-intensity pulsed electric fields. | to apply scanning electron microscopy, image analysis and a fluorescent viability stain to assess lethal and sublethal in food-borne bacteria exposed to high-intensity pulsed electric fields (pef). | 2004 | 15287869 |
| genetic and functional analysis of the cytk family of genes in bacillus cereus. | cytk is a pore-forming toxin of bacillus cereus that has been linked to a case of necrotic enteritis. pcr products of the expected size were generated with cytk primers in 13 of 29 strains. six strains were pcr-positive for the related gene hly-ii, which encodes haemolysin ii, a protein that is 37 % identical to the original cytk. five of the strains were positive for both genes. the dna sequences of putative cytk genes from three positive strains were determined, and the deduced amino acid sequ ... | 2004 | 15289565 |
| comparison of cytotoxin cytk promoters from bacillus cereus strain atcc 14579 and from a b. cereus food-poisoning strain. | the cytotoxin cytk produced by bacillus cereus is believed to be involved in food-borne diseases. the transcriptional activity of the cytk promoter region in a food-poisoning strain was studied using a reporter gene and compared with that in the reference b. cereus strain atcc 14579. in the food-poisoning strain, cytk is more strongly transcribed, possibly explaining the pathogenicity. the global regulator plcr in b. cereus controls several putative virulence factors. it was found that plcr regu ... | 2004 | 15289566 |
| laundry detergent compatibility of the alkaline protease from bacillus cereus. | the endogenous protease activity in various commercially available laundry detergents of international companies was studied. the maximum protease activity was found at 50 degrees c in ph range 10.5-11.0 in all the tested laundry detergents. the endogenous protease activity in the tested detergents retained up to 70% on incubation at 40 degrees c for 1 h, whereas less than 30% activity was only found on incubation at 50 degrees c for 1 h. the alkaline protease from an alkalophilic strain of baci ... | 2004 | 15293947 |
| mics of selected antibiotics for bacillus anthracis, bacillus cereus, bacillus thuringiensis, and bacillus mycoides from a range of clinical and environmental sources as determined by the etest. | this paper presents etest determinations of mics of selected antimicrobial agents for 76 isolates of bacillus anthracis chosen for their diverse histories and 67, 12, and 4 cultures, respectively, of its close relatives b. cereus, b. thuringiensis, and b. mycoides derived from a range of clinical and environmental sources. nccls breakpoints are now available for b. anthracis and ciprofloxacin, penicillin, and tetracycline; based on these breakpoints, the b. anthracis isolates were all fully susc ... | 2004 | 15297508 |
| use of 16s rrna, 23s rrna, and gyrb gene sequence analysis to determine phylogenetic relationships of bacillus cereus group microorganisms. | in order to determine if variations in rrna sequence could be used for discrimination of the members of the bacillus cereus group, we analyzed 183 16s rrna and 74 23s rrna sequences for all species in the b. cereus group. we also analyzed 30 gyrb sequences for b. cereus group strains with published 16s rrna sequences. our findings indicated that the three most common species of the b. cereus group, b. cereus, bacillus thuringiensis, and bacillus mycoides, were each heterogeneous in all three gen ... | 2004 | 15297521 |
| assessment of a new selective chromogenic bacillus cereus group plating medium and use of enterobacterial autoinducer of growth for cultural identification of bacillus species. | a new chromogenic bacillus cereus group plating medium permits differentiation of pathogenic bacillus species by colony morphology and color. probiotic b. cereus mutants were distinguished from wild-type strains by their susceptibilities to penicillin g or cefazolin. the enterobacterial autoinducer increased the sensitivity and the speed of enrichment of b. cereus and b. anthracis spores in serum-supplemented minimal salts medium (based on the standard american petroleum institute medium) and bu ... | 2004 | 15297532 |
| bacillus cereus pneumonia in a patient with acute lymphoblastic leukemia. | reported here is a case of bacillus cereus pneumonia that occurred in a patient with acute lymphoblastic leukemia. the presentation was severe, essentially marked by respiratory distress and pleuritic chest pain. classic empirical treatment initiated for febrile neutropenia did not cover this rare pathogen and appropriate therapy was therefore delayed. b. cereus is most often a culture contaminant, but it can also be responsible for self-limited gastrointestinal intoxication and, more rarely, se ... | 2004 | 15300457 |
| [obtaining of intrapopulational dissociants of some bacilli and the use of dir-pcr for their identification]. | the paper is the first to suggest methods for rapid obtaining and genotypic identification of phenotypic (colonial-morphological) dissociants of bacterial cultures. for revelation of the potential dissociation ability and obtaining of dissociants, the use of bacterial cyst-like refractile cells (crc) is recommended. these cells are characterized by enhanced variability; upon their first passage, an abrupt increase in the dissociation index is observed as a result of the emergence of cells that f ... | 2004 | 15315235 |
| antimicrobial activity and cytotoxicity of the essential oil of curcuma zedoaria. | the chemical compositions of the essential oil of curcuma zedoaria (berg.) rosc. were analyzed by gas chromatography-mass spectrometry (gc-ms) and showed a high content of epicurzerenone and curdione representing 46.6% and 13.7% of the total oil, respectively. the essential oil was evaluated for potential antimicrobial activity against staphylococcus aureus, escherichia coli, pseudomonasa aeruginosa, vibrio parahaemolyticus, salmonella typhimurium and bacillus cereus. v. parahaemolyticus was sen ... | 2004 | 15315265 |
| validation of ph and glucose determination for bacteria detection screening in platelet concentrates stored in the terumo teruflex xt612 platelet container. | 2004 | 15318869 | |
| population structure of the bacillus cereus group as determined by sequence analysis of six housekeeping genes and the plcr gene. | the population structure of the bacillus cereus group (52 strains of b. anthracis, b. cereus, and b. thuringiensis) was investigated by sequencing seven gene fragments (rpob, gyrb, pyca, mdh, mbl, muts, and plcr). most of the strains were classifiable into two large subgroups in six housekeeping gene trees but not in the plcr tree. in addition, several consistent clusters were identified, which were unrelated to species distinction. moreover, interrelationships among these clusters were incongru ... | 2004 | 15322020 |
| evaluation of antibacterial properties of some medicinal plants used in iran. | forty-five species of 29 plant families used in the traditional medicine by iranian people, showed antibacterial activities against one or more of the bacterial species: bacillus cereus, bacillus pumilus, bordetella bronchiseptica, escherichia coli, klebsiella pneumoniae, micrococcus luteus, pseudomonas aeruginosa, pseudomonas fluorescens, serratia marcescens, staphylococcus aureus and staphylococcus epidermidis. no plant showed activity against serratia marcescens; bordetella bronchiseptica bei ... | 2004 | 15325735 |
| a pcr assay based on a sequence-characterized amplified region marker for detection of emetic bacillus cereus. | a pcr assay for the detection of bacillus cereus strains able to produce an emetic toxin (cereulide) was developed in this study based on a sequence-characterized amplified region (scar) derived from a random amplified polymorphic dna (rapd) fragment. one of the rapd fragments generated was selected, cloned, and sequenced. a set of pcr primers was newly designed from the scar obtained (the sequence of the cloned rapd fragment) and used in this assay. to determine the specificity of the assay, 30 ... | 2004 | 15330536 |
| lethality of chlorine, chlorine dioxide, and a commercial fruit and vegetable sanitizer to vegetative cells and spores of bacillus cereus and spores of bacillus thuringiensis. | chlorine, clo2, and a commercial raw fruit and vegetable sanitizer were evaluated for their effectiveness in killing vegetative cells and spores of bacillus cereus and spores of bacillus thuringiensis. the ultimate goal was to use one or both species as a potential surrogate(s) for bacillus anthracis in studies that focus on determining the efficacy of sanitizers in killing the pathogen on food contact surfaces and foods. treatment with alkaline (ph 10.5 to 11.0) clo2 (200 microg/ml) produced by ... | 2004 | 15330537 |
| detection of bacillus cereus on selected retail chicken products. | samples from five chicken meat products, obtained at retail stores, were evaluated for the presence of bacillus cereus. the products tested were as follows: breaded, fully cooked, frozen nuggets (nuggets); breaded, fully cooked, frozen tenders (tenders); fully cooked, frozen, white-meat fajita-style strips (strips); raw, refrigerated, boneless, skinless, marinated breast fillets (fillets); and raw, refrigerated, cut-up, tray-pack bone-in parts (parts), either split breasts or thighs. four packag ... | 2004 | 15330548 |
| antibacterial activities of naturally occurring compounds against antibiotic-resistant bacillus cereus vegetative cells and spores, escherichia coli, and staphylococcus aureus. | after demonstrating the lack of effectiveness of standard antibiotics against the acquired antibiotic resistance of bacillus cereus (nctc 10989), escherichia coli (nctc 1186), and staphylococcus aureus (atcc 12715), we showed that the following natural substances were antibacterial against these resistant pathogens: cinnamon oil, oregano oil, thyme oil, carvacrol, (s)-perillaldehyde, 3,4-dihydroxybenzoic acid (beta-resorcylic acid), and 3,4-dihydroxyphenethylamine (dopamine). exposure of the thr ... | 2004 | 15330549 |
| colony immunoblot assay for the detection of hemolysin bl enterotoxin producing bacillus cereus. | bacillus cereus strains involved in food poisoning cases of the diarrheal type may produce two different enterotoxin complexes. to facilitate the identification of hemolysin bl-enterotoxin complex (hbl) and/or the nonhemolytic enterotoxin (nhe) producing colonies a colony immunoblot procedure was developed, which allows a fast and easy identification of the respective colonies from blood agar plates. the enterotoxins were transferred from the blood agar medium to a nitrocellulose membrane and th ... | 2004 | 15336410 |
| genotyping and toxigenic potential of bacillus subtilis and bacillus pumilus strains occurring in industrial and artisanal cured sausages. | artisanal and industrial sausages were analyzed for their aerobic, heat-resistant microflora to assess whether new emerging pathogens could be present among bacillus strains naturally contaminating cured meat products. sixty-four isolates were characterized by randomly amplified polymorphic dna (rapd)-pcr and fluorescent amplified fragment length polymorphism (faflp). the biotypes, identified by partial 16s rrna gene sequence analysis, belonged to bacillus subtilis, bacillus pumilus, and bacillu ... | 2004 | 15345396 |
| anaerobic growth of bacillus mojavensis and bacillus subtilis requires deoxyribonucleosides or dna. | bacillus mojavensis strains jf-2 (atcc 39307), rob2, and abo21191(t) and bacillus subtilis strains 168 (atcc 23857) and atcc 12332 required four deoxyribonucleosides or dna for growth under strict anaerobic conditions. bacillus licheniformis strains l89-11 and l87-11, bacillus sonorensis strain tg8-8, and bacillus cereus (atcc 14579) did not require dna for anaerobic growth. the requirement for the deoxyribonucleosides or dna did not occur under aerobic growth conditions. the addition of a mixtu ... | 2004 | 15345407 |
| detection and characterization of beta-lactam resistance in bacillus cereus ptcc 1015. | in the present study, detection, isolation, and characterization of beta-lactamases from bacillus cereus ptcc 1015 were investigated. b. cereus was inoculated in nutrient broth containing ampicillin (50 mg x ml(-1)) for 24 h (35 degrees c, 200 rpm). activity measurements were carried out against ampicillin (0.1 mg x ml(-1)) and cephalexin (0.08 mg x ml(-1)) by a spectrophotometric method at different conditions (ph 6-10, temperatures 25-45 degrees c). maximum penicillinase and cephalosporinase a ... | 2004 | 15349504 |
| the nhea component of the non-hemolytic enterotoxin of bacillus cereus is produced by bacillus anthracis but is not required for virulence. | a non-hemolytic enterotoxin (nhe) is one of the two enterotoxins thought to cause diarrhea produced by bacillus cereus. we identified genes in bacillus anthracis homologous to the b. cereus nheab genes encoding proteins of the nhe complex. the nhea component was detected immunologically in culture supernatants from b. anthracis but not from a nhea(-) mutant, suggesting that b. anthracis produces and secretes the nhea subunit of nhe. a nhea deletion mutant was not attenuated in the guinea pig sug ... | 2004 | 15351038 |
| atmospheric oxygen and other conditions affecting the production of cereulide by bacillus cereus in food. | factors influencing the production of cereulide, the emetic toxin of bacillus cereus in food and laboratory media were investigated, using liquid chromatography-ion trap mass spectrometry and sperm motility inhibition bioassay for detection and quantitation. oxygen was essential for production of the emetic toxin by b. cereus. when beans, rice or tryptic soy broth were inoculated with cereulide producing strains b203, b116 (recent food isolates) or the strain f-4810/72, high amounts (2 to 7 micr ... | 2004 | 15358508 |
| glycopeptide derived from hen egg ovomucin has the ability to bind enterohemorrhagic escherichia coli o157:h7. | ovomucin glycopeptide (ogp) was prepared by size exclusion chromatography after pronase digestion of hen egg ovomucin, and the binding of ogp to foodborne pathogens (bacillus cereus,clostridium perfringens, escherichia coli o157:h7, listeria monocytogenes, salmonella enteritidis, salmonella typhimurium, and staphylococcus aureus) was investigaed. binding assays with biotinylated bacteria as probes in microtiter plates showed that ogp bound to only e. coli o157:h7 among these foodborne pathogens. ... | 2004 | 15373418 |
| mesophilic aerobic degradation of a metal lubricant by a biological consortium. | the metal-forming industries require the use of greases to lubricate metal surfaces during manufacturing operations, and the residues of these lubricants must be removed prior to finishing processes to protect and improve the appearance of the final product. an aqueous, biological metal-cleaning process operating under mild conditions (ph 9, 42 degrees c) eliminates the use of environmentally unfriendly cleaning materials such as chlorinated solvents by employing microorganisms to degrade grease ... | 2004 | 15378296 |
| a targeted proteomics approach to the rapid identification of bacterial cell mixtures by matrix-assisted laser desorption/ionization mass spectrometry. | a proteomic approach to the rapid identification of bacteria is presented, which relies on the solubilization of a limited number of proteins from intact cells combined with on-probe tryptic digestion. within 20 min, complete cleavage products of a limited set of bacterial proteins with molecular masses of about 4-125 kda were obtained by on-probe digestion with immobilized trypsin. bacterial peptides suitable for unimolecular decomposition analysis were generated within 5 min, and the sequence ... | 2004 | 15378756 |
| indigenous bacteria with antagonistic and plant-growth-promoting activities improve slow-filtration efficiency in soilless cultivation. | in tomato soilless culture, slow filtration allows one to control the development of diseases caused by pathogenic microorganisms. during the disinfecting process, microbial elimination is ensured by mechanical and biological factors. in this study, system efficacy was enhanced further to a biological activation of filter by inoculating the pozzolana grains contained in the filtering unit with 5 selected bacteria. three strains identified as pseudomonas putida and 2 as bacillus cereus came from ... | 2004 | 15381975 |
| photochemical treatment of platelet concentrates with amotosalen and long-wavelength ultraviolet light inactivates a broad spectrum of pathogenic bacteria. | bacterial contamination of platelet (plt) concentrates can result in transfusion-transmitted sepsis. a photochemical treatment (pct) process with amotosalen hcl and long-wavelength ultraviolet light (uva), which cross-links nucleic acids, was developed to inactivate bacteria and other pathogens in plt concentrates. | 2004 | 15383024 |
| a spontaneous translational fusion of bacillus cereus plcr and papr activates transcription of plcr-dependent genes in bacillus anthracis via binding with a specific palindromic sequence. | transformation of bacillus anthracis with plasmid pute29-plcr-papr carrying the native bacillus cereus plcr-papr gene cluster did not activate expression of b. anthracis hemolysin genes, even though these are expected to be responsive to activation by the global regulator plcr. to further characterize the action of plcr, we examined approximately 3,000 b. anthracis transformants containing pute29-plcr-papr and found a single hemolytic colony. the hemolytic strain contained a plasmid having a spo ... | 2004 | 15385482 |
| characterization and virulence of hemolysin iii from vibrio vulnificus. | vibrio vulnificus, a highly virulent marine bacterium, is the causative agent of both serious wound infections and fatal septicemia in many areas of the word. a gene (hlyiii) encoding a hemolysin was cloned and sequenced from v. vulnificus. nucleotide sequence analysis predicted an open reading frame of 642 bp encoding a 214 amino acid polypeptide that showed 48% sequence identity to the hemolysin iii of bacillus cereus. when hlyiii of v. vulnificus was expressed in escherichia coli, crude extra ... | 2004 | 15386100 |
| inhibition of beta-lactamase ii of bacillus cereus by penamaldic derivatives of penicillins. | the penamaldic derivatives of amoxicillin, ampicillin, and penicillins g and v, stabilized with zn(2+), were obtained from a methanolic medium. the enzymatic kinetic results show that the these derivatives elicit reversible inhibition of the enzyme metallo-beta-lactamase from bacillus cereus, with inhibition constant values determined at ph 7.0 and 25 degrees c. | 2004 | 14982810 |
| glycosylphosphatidylinositol (gpi) proteins of saccharomyces cerevisiae contain ethanolamine phosphate groups on the alpha1,4-linked mannose of the gpi anchor. | in humans and saccharomyces cerevisiae the free glycosylphosphatidylinositol (gpi) lipid precursor contains several ethanolamine phosphate side chains, but these side chains had been found on the protein-bound gpi anchors only in humans, not yeast. here we confirm that the ethanolamine phosphate side chain added by mcd4p to the first mannose is a prerequisite for the addition of the third mannose to the gpi precursor lipid and demonstrate that, contrary to an earlier report, an ethanolamine phos ... | 2004 | 14985347 |
| two distinct types of rrna operons in the bacillus cereus group. | the bacillus cereus group includes insecticidal bacteria (b. thuringiensis), food-borne pathogens (b. cereus and b. weihenstephanensis) and b. anthracis, the causative agent of anthrax. the precise number of rrna operons in 12 strains of the b. cereus group was determined. most of the tested strains possess 13 operons and the tested psychrotolerant strains contain 14 operons, the highest number ever found in bacteria. the separate clustering of the tested psychrotolerant strains was confirmed by ... | 2004 | 14993309 |
| evaluation of a new generation of plastic culture bottles with an automated microbial detection system for nine common contaminating organisms found in plt components. | a microbial detection system (bact/alert 3d, biomérieux [formerly organon teknika]) has previously been validated with a variety of bacterial contaminants in plts. the recovery of nine organisms seeded into plts with new plastic culture bottles was studied in comparison to the current glass bottles. the use of plastic instead of glass would be expected to reduce the risk of injury. | 2004 | 14996192 |
| analysis of the substrate specificity loop of the had superfamily cap domain. | the haloacid dehalogenase (had) superfamily includes a variety of enzymes that catalyze the cleavage of substrate c-cl, p-c, and p-op bonds via nucleophilic substitution pathways. all members possess the alpha/beta core domain, and many also possess a small cap domain. the active site of the core domain is formed by four loops (corresponding to sequence motifs 1-4), which position substrate and cofactor-binding residues as well as the catalytic groups that mediate the "core" chemistry. the cap d ... | 2004 | 15005616 |
| characterization of a broad range antimicrobial substance from bacillus cereus. | the aim of this research was to isolate and characterize an antimicrobial substance from the bacillus cereus type strain atcc 14579. | 2004 | 15012801 |
| assessment of bacterial endospore viability with fluorescent dyes. | to validate three fluorescence viability assays designed primarily for vegetative cells on pure bacillus endospores. | 2004 | 15012806 |
| estimating disease resistance in insects: phenoloxidase and lysozyme-like activity and disease resistance in the cricket gryllus texensis. | an animal's ability to resist disease is usually estimated by measuring one or more components of the immune system. there is an assumption that these assays of immunity measure an animal's ability to mount an effective immune response. this paper tests this assumption by examining the relationship between two common estimates of insect immunocompetence, phenoloxidase and lysozyme-like enzyme activity, and resistance to three common insect bacterial pathogens: serratia marcescens, serratia lique ... | 2004 | 15019523 |
| the inhibition of metallo-beta-lactamase by thioxo-cephalosporin derivatives. | the 8-thioxocephalosporins are poor substrates for the b. cereus metallo beta-lactamase (k(cat)/k(m)=61.4m(-1) s(-1)) and act as weak competitive inhibitors (k(i) approximately 700 microm). the hydrolysis product of thioxocephalosporin, a thioacid, also inhibits the enzyme competitively with a k(i)=96 microm, whereas the cyclic thioxo-piperazinedione, formed by intramolecular aminolysis of thioxocephalexin has a k(i) of 29 microm. | 2004 | 15026061 |
| identification of bacillus anthracis specific chromosomal sequences by suppressive subtractive hybridization. | bacillus anthracis, bacillus thuringiensis and bacillus cereus are closely related members of the b. cereus-group of bacilli. suppressive subtractive hybridization (ssh) was used to identify specific chromosomal sequences unique to b. anthracis. | 2004 | 15028116 |
| bacillus anthracis and bacillus cereus pcra helicases can support dna unwinding and in vitro rolling-circle replication of plasmid pt181 of staphylococcus aureus. | replication of rolling-circle replicating (rcr) plasmids in gram-positive bacteria requires the unwinding of initiator protein-nicked plasmid dna by the pcra helicase. in this report, we demonstrate that heterologous pcra helicases from bacillus anthracis and bacillus cereus are capable of unwinding staphylococcus aureus plasmid pt181 from the initiator-generated nick and promoting in vitro replication of the plasmid. these helicases also physically interact with the repc initiator protein of pt ... | 2004 | 15028705 |
| antimicrobial activity of artemisia douglasiana leaf essential oil. | artemisia douglasiana leaf has been shown to be efficacious complementary herbal treatment for chronic bladder infection in a paraplegic youth. the leaf oil has been analyzed by gc-ms and the major components found to be camphor (29%), artemisia ketone (26%), artemisia alcohol (13%), alpha-thujone (10%), 1,8-cineole (8%), and hexanal (5%). the leaf oil and the major components have been tested for antimicrobial activity against bacillus cereus, staphylococcus aureus, escherichia coli, pseudomona ... | 2004 | 15030924 |
| antibacterial screening of plants used in iranian folkloric medicine. | fifty methanolic plant extracts belonging to 44 plant species of 33 families finding use in iranian folkloric medicine were screened for antibacterial activity. thirty samples, including 28 species in 20 families, had antibacterial activity against at least on one of the bacteria. among the active plants, 32.6% were active against g(-), 62% against g(+), and 47.3% against both g(-) and g(+) bacteria. dianthus coryophyllus was active against all tested g(-) and g(+) bacteria except micrococcus lu ... | 2004 | 15030933 |
| identification of emetic toxin producing bacillus cereus strains by a novel molecular assay. | bacillus cereus causes two types of gastrointestinal diseases: emesis and diarrhea. the emetic type of the disease is attributed to the heat-stable depsipeptide cereulide and symptoms resemble staphylococcus aureus intoxication, but there is no rapid method available to detect b. cereus strains causing this type of disease. in this study, a polymerase chain reaction (pcr) fragment of unknown function was identified, which was shown to be specific for emetic toxin producing strains of b. cereus. ... | 2004 | 15033238 |
| rapid dipicolinic acid extraction from bacillus spores detected by surface-enhanced raman spectroscopy. | 2004 | 15035719 | |
| influence of media and temperature on bacteriocin production by bacillus cereus 8a during batch cultivation. | cerein 8a is a bacteriocin produced by the soil bacterium bacillus cereus 8a, isolated from native woodlands of brazil. the influence of temperature and media on the growth of b. cereus 8a and the production of this bacteriocin was studied during batch cultivation. maximum activity was detected by cultivation in brain/heart infusion broth, reaching 3200 activity units ml(-1). bacteriocin was also produced in peptone, mrs, mueller-hinton and nutrient broth, while no activity was observed during c ... | 2004 | 14966665 |
| phosphatidylinositol-specific phospholipase c forms different complexes with monodisperse and micellar phosphatidylcholine. | phosphatidylinositol-specific phospholipase c (pi-plc) from bacillus cereus forms a premicellar complex e(#) with monodisperse diheptanoylphosphatidylcholine (dc(7)pc) that is distinguishable from the e complex formed with micelles. results are interpreted with the assumption that in both cases amphiphiles bind to the interfacial binding surface (i-face) of pi-plc but not to the active site. isothermal calorimetry and fluorescence titration results for the binding of monodisperse dc(7)pc give an ... | 2004 | 14967048 |
| gyrb as a phylogenetic discriminator for members of the bacillus anthracis-cereus-thuringiensis group. | bacillus anthracis, the causative agent of the human disease anthrax, bacillus cereus, a food-borne pathogen capable of causing human illness, and bacillus thuringiensis, a well-characterized insecticidal toxin producer, all cluster together within a very tight clade (b. cereus group) phylogenetically and are indistinguishable from one another via 16s rdna sequence analysis. as new pathogens are continually emerging, it is imperative to devise a system capable of rapidly and accurately different ... | 2004 | 14967230 |
| a colony blot immunoassay for the rapid identification of bacillus cereus. | a colony blot immunoassay was developed for the rapid identification of bacillus cereus using antibodies against the 28.5-kda cell-surface antigen of b. cereus. suspect colonies from plates were blotted onto a whatman #541 membrane, dried, and fixed by uv irradiation. the membrane was then immersed in an anti-b. cereus antibody-horseradish peroxidase conjugate for 60 min. after washing and reacting with 4-chloro-1-naphthol and h2o2, the appearance of purple spots indicated the presence of b. cer ... | 2004 | 14968975 |
| antimicrobial activity of a low-molecular-weight chitosan obtained from cellulase digestion of chitosan. | a water-soluble chitosan hydrolysate with high activity against escherichia coli was obtained during cellulase digestion of chitosan for 18 h. this 18-h hydrolysate is composed of low-molecular-weight chitosan (lmwc), with a molecular weight of 12.0 kda, and chitooligosaccharides, which are composed of sugars with a degree of polymerization of 1 to 8. lmwc has a strong activity at 100 ppm against many pathogens and yeast species, including bacillus cereus, e. coli, staphylococcus aureus, pseudom ... | 2004 | 14968977 |
| volatile constituents from the leaves of callicarpa japonica thunb. and their antibacterial activities. | volatile substances of callicarpa japonica thunb. were examined for their antibacterial activities against six foodborne microorganisms using the optical densitometer bioscreen c. extracts of c. japonica were obtained by simultaneous steam distillation and solvent extraction (sde), and those extracted for 1.5 and 2.0 h at ph 6.0 strongly inhibited the growth of bacillus cereus and salmonella typhimurium; the content of the volatile substances of leaves at these ph levels were 543.1 and 706.7 mg/ ... | 2004 | 14969531 |
| a new intrinsic thermal parameter for enzymes reveals true temperature optima. | two established thermal properties of enzymes are the arrhenius activation energy and thermal stability. arising from anomalies found in the variation of enzyme activity with temperature, a comparison has been made of experimental data for the activity and stability properties of five different enzymes with theoretical models. the results provide evidence for a new and fundamental third thermal parameter of enzymes, t(eq), arising from a subsecond timescale-reversible temperature-dependent equil ... | 2004 | 14973131 |
| essential oils: their antibacterial properties and potential applications in foods--a review. | in vitro studies have demonstrated antibacterial activity of essential oils (eos) against listeria monocytogenes, salmonella typhimurium, escherichia coli o157:h7, shigella dysenteria, bacillus cereus and staphylococcus aureus at levels between 0.2 and 10 microl ml(-1). gram-negative organisms are slightly less susceptible than gram-positive bacteria. a number of eo components has been identified as effective antibacterials, e.g. carvacrol, thymol, eugenol, perillaldehyde, cinnamaldehyde and cin ... | 2004 | 15246235 |
| identification of bacillus anthracis by multiprobe microarray hybridization. | we have developed a rapid assay based on microarray analysis of amplified genetic markers for reliable identification of bacillus anthracis and its discrimination from other closely related bacterial species of the bacillus cereus group. by combining polymerase chain reaction (pcr) amplification of six b. anthracis-specific genes (plasmid-associated genes encoding virulence factors (cyaa, paga, lef, and capa, capb, capc) and one chromosomal marker ba-5449) with analysis of amplicons by microarra ... | 2004 | 15246505 |
| mercuric resistance genes in gram-positive oral bacteria. | mercury-resistant bacteria isolated from the oral cavities of children carried one of two types of mera gene that appear to have evolved from a common ancestor. streptococcus oralis, streptococcus mitis and a few other species had mera genes that were very similar to mera of bacillus cereus strain rc607. unlike the b. cereus rc607 mera gene, however, the streptococcal mera genes were not carried on tn5084-like transposons. instead, comparisons with microbial genomic sequences suggest the mera ge ... | 2004 | 15251199 |
| evidence for non-ribosomal peptide synthetase production of cereulide (the emetic toxin) in bacillus cereus. | little is known about the process whereby the emetic toxin (or cereulide) of bacillus cereus is produced. two cereulide-producing strains of b. cereus were cloned and sequenced following polymerase chain reaction (pcr) amplification with primers that were specific for conserved regions of non-ribosomal peptide synthetase (nrps) genes. the cloned regions of the b. cereus strains were highly homologous to conserved regions of other peptide synthetase nucleotide sequences. primers were designed for ... | 2004 | 15251214 |
| the structure of the cell wall peptidoglycan of bacillus cereus rsvf1, a strain closely related to bacillus anthracis. | the peptidoglycan of bacillus cereus rsvf1, a close relative of bacillus anthracis, has several distinguishing features: the overwhelming majority of cross-linked muropeptides are dimers, higher oligomers are only present in minute quantities; and virtually all muropeptides lack the n-acetyl group from glucosamine residues, thus explaining resistance of the cell walls to lysozyme. | 2004 | 15256021 |
| differences among antimicrobial properties of carrion beetle secretions reflect phylogeny and ecology. | carrion beetles (coleoptera: silphidae) consist of two subfamilies in north america. members of the silphinae arrive at carcasses during the mid-stage of decay and their larvae feed on developing maggots, while members of the nicrophorinae bury and tend carcasses upon which their developing larvae feed. the nicrophorinae maintain the condition of the carcass by applying oral and anal secretions that reduce carcass decay apparently through bacterial inhibition, although quantification has not bee ... | 2004 | 15260219 |
| the genome sequence of bacillus cereus atcc 10987 reveals metabolic adaptations and a large plasmid related to bacillus anthracis pxo1. | we sequenced the complete genome of bacillus cereus atcc 10987, a non-lethal dairy isolate in the same genetic subgroup as bacillus anthracis. comparison of the chromosomes demonstrated that b.cereus atcc 10987 was more similar to b.anthracis ames than b.cereus atcc 14579, while containing a number of unique metabolic capabilities such as urease and xylose utilization and lacking the ability to utilize nitrate and nitrite. additionally, genetic mechanisms for variation of capsule carbohydrate an ... | 2004 | 14960714 |
| phenolic metabolites of dalea versicolor that enhance antibiotic activity against model pathogenic bacteria. | a new flavonoid (1) was isolated from organic extracts of dalea versicolor, along with six known phenolic compounds (2-7). the structures of the seven compounds were determined by nmr and hrms methods. these compounds were evaluated for direct activity against a variety of organisms in vitro, including the gram-positive bacteria staphylococcus aureus and bacillus cereus. in addition, the compounds were evaluated for their ability to potentiate the activity of known antimicrobials through inhibit ... | 2004 | 15043439 |
| novel inhibition mechanism of bacillus cereus sphingomyelinase by beryllium fluoride. | phosphate analogs have been known to inhibit competitively various phosphatases and phospholipase c and d. we found for the first time that only beryllium fluoride (bef(x)) among the phosphate analogs studied inhibits bacillus cereus sphingomyelinase (smase) activity. the active inhibitory species proved to be not bef(3)(-) but bef(2) by the measurement of smase activity and of (19)f nmr spectroscopy in the presence of a fixed concentration of becl(2) and different concentrations of naf, althoug ... | 2004 | 15047192 |
| circular dichroism of protein-folding intermediates. | 2004 | 15051341 | |
| rational design of a novel fluorescent biosensor for beta-lactam antibiotics from a class a beta-lactamase. | a rational design strategy was used to construct a sensitive "turn-on" biosensor for beta-lactam antibiotics and beta-lactamase inhibitors from a class a beta-lactamase mutant with suppressed hydrolytic activity. a fluorescein molecule was attached to the 166 position on the omega-loop of the e166c mutant close to the active site of the beta-lactamase. upon binding with antibiotics or inhibitors, the flexibility of the omega-loop allows the fluorescein molecule to move out from the active site a ... | 2004 | 15053574 |
| characterization of a major bacillus anthracis spore coat protein and its role in spore inactivation. | a major bacillus anthracis spore coat protein of 13.4 kda, designated cot alpha, was found only in the bacillus cereus group. a stable ca. 30-kda dimer of this protein was also present in spore coat extracts. cot alpha, which is encoded by a monocistronic gene, was first detected late in sporulation, consistent with a sigma(k)-regulated gene. on the basis of immunogold labeling, the protein is in the outer spore coat and absent from the exosporium. in addition, disruption of the gene encoding co ... | 2004 | 15060044 |
| the 26 nudix hydrolases of bacillus cereus, a close relative of bacillus anthracis. | the genome of bacillus cereus contains 26 nudix hydrolase genes, second only to its closest relative, bacillus anthracis which has 30. all 26 genes have been cloned, 25 have been expressed, and 21 produced soluble proteins suitable for analysis. substrates for 16 of the enzymes were identified; these included adp-ribose, diadenosine polyphosphates, sugar nucleotides, and deoxynucleoside triphosphates. one of the enzymes was a cdp-choline pyrophosphatase, the first nudix hydrolase active on this ... | 2004 | 15060060 |
| a napin-like polypeptide from dwarf chinese white cabbage seeds with translation-inhibitory, trypsin-inhibitory, and antibacterial activities. | napins are 1:1 disulfide-linked complexes of a smaller (ca. 4kda) subunit and a larger (ca. 10kda) subunit. the intent of the present study was to ascertain the production of napin by the seeds of a brassica species that has not been examined previously, and also to explore new biological activities of the napin. a heterodimeric 11-kda napin-like polypeptide has been isolated from chinese white cabbage (brassica chinensis cv dwarf) seeds with a protocol comprising ion exchange chromatography on ... | 2004 | 15062997 |
| detection of bacillus cereus group bacteria from cardboard and paper with real-time pcr. | the aim of this study was to develop a pcr-based rapid method to detect bacillus cereus group cells from paper and cardboard. primers targeting the 16s rdna and real-time pcr with sybr green i detection were used in order to be able to also quantify the target. both autoclaved cardboard samples spiked with b. cereus vegetative cells or spores and naturally contaminated paper and cardboard samples were studied. results were compared with culturing verified by commercial (api) tests. several diffe ... | 2004 | 15064974 |
| characterization of bacillus probiotics available for human use. | bacillus species (bacillus cereus, bacillus clausii, bacillus pumilus) carried in five commercial probiotic products consisting of bacterial spores were characterized for potential attributes (colonization, immunostimulation, and antimicrobial activity) that could account for their claimed probiotic properties. three b. cereus strains were shown to persist in the mouse gastrointestinal tract for up to 18 days postadministration, demonstrating that these organisms have some ability to colonize. s ... | 2004 | 15066809 |
| growth and sporulation of bacillus cereus atcc 14579 under defined conditions: temporal expression of genes for key sigma factors. | an airlift fermentor system allowing precise regulation of ph and aeration combined with a chemically defined medium was used to study growth and sporulation of bacillus cereus atcc 14579. sporulation was complete and synchronous. expression of siga, sigb, sigf, and sigg was monitored with real-time reverse transcription-pcr, and the pattern qualitatively resembled that of bacillus subtilis. this method allows reproducible production of stable spores, while the synchronous growth and defined con ... | 2004 | 15066852 |
| shelf life and safety concerns of bakery products--a review. | bakery products are an important part of a balanced diet and, today, a wide variety of such products can be found on supermarket shelves. this includes unsweetened goods (bread, rolls, buns, crumpets, muffins and bagels), sweet goods (pancakes, doughnuts, waffles and cookies) and filled goods (fruit and meat pies, sausage rolls, pastries, sandwiches, cream cakes, pizza and quiche). however, bakery products, like many processed foods, are subject to physical, chemical and microbiological spoilage ... | 2004 | 15077880 |
| cytotoxic bacillus spp. belonging to the b. cereus and b. subtilis groups in norwegian surface waters. | to investigate the presence and numbers of bacillus spp. spores in surface waters and examine isolates belonging to the b. cereus and b. subtilis groups for cytotoxicity, and to discuss the presence of cytotoxic bacillus spp. in surface water as hazard identification in a risk assessment approach in the food industry. | 2004 | 15078515 |
| influence of surface chemistry on the hygienic status of industrial stainless steel. | coupons of fourteen different stainless steels were investigated in terms of surface chemistry and ease of cleaning. steel surfaces were exposed to bacillus cereus spores in static saline solution for 2 h. surfaces were rinsed and then covered with whole milk and allowed to dry. surfaces were then cleaned in an experimental flow system that mimics an industrial application. after cleaning, remaining spores were released by sonication, spores cultured and colony forming units determined. surfaces ... | 2004 | 15079890 |
| identification of pathogenic microbial cells and spores by electrochemical detection on a biochip. | background: bacillus cereus constitutes a significant cause of acute food poisoning in humans. despite the recent development of different detection methods, new effective control measures and better diagnostic tools are required for quick and reliable detection of pathogenic micro-organisms. thus, the objective of this study was to determine a simple method for rapid identification of enterotoxic bacillus strains. here, a special attention is given to an electrochemical biosensor since it meets ... | 2004 | 15090067 |
| isolation of a minireplicon of the virulence plasmid pxo2 of bacillus anthracis and characterization of the plasmid-encoded reps replication protein. | a minireplicon of plasmid pxo2 of bacillus anthracis was isolated by molecular cloning in escherichia coli and shown to replicate in b. anthracis, bacillus cereus, and bacillus subtilis. the pxo2 replicon included (i) an open reading frame encoding the putative reps replication initiation protein and (ii) the putative origin of replication. the reps protein was expressed as a fusion with the maltose binding protein (mbp) at its amino-terminal end and purified by affinity chromatography. electrop ... | 2004 | 15090513 |
| composition and bioactivity of the leaf essential oil of heteropyxis dehniae from zimbabwe. | the leaf oil of heteropyxis dehniae suess. (heteropyxidaceae) was obtained by hydrodistillation and analyzed by gc/ms. the most abundant essential oil components are linalool (58.3%), 4-terpineol (9.8%), alpha-terpineol (3.6%), and caryophyllene oxide (3.1%). the antimicrobial activity against bacillus cereus, staphylococcus aureus, pseudomonas aeruginosa, escherichia coli, candida albicans, and aspergillus niger, and the in vitro cytotoxicity of the oil on pc-3, mda-mb-231, hs 578t, mcf7, sk-me ... | 2004 | 15099856 |
| new chromanone acids with antibacterial activity from calophyllum brasiliense. | six novel chromanone acids (1-6) were isolated from the bark of calophyllum brasiliense cambess. their structures were elucidated on the basis of 1d and 2d nmr experiments, as well as mass spectrometry. all compounds showed moderate to strong antibacterial activity against bacillus cereus and staphylococcus epidermidis, with 1 and 2 being most active. none of the compounds were cytotoxic against kb, jurkat t, and myosarcoma cancer cells up to 20 microg/ml. | 2004 | 15104480 |
| rugulotrosins a and b: two new antibacterial metabolites from an australian isolate of a penicillium sp. | two new antibacterial agents, rugulotrosin a (1) and b (2), were obtained from cultures of a penicillium sp. isolated from soil samples acquired near sussex inlet, new south wales, australia. rugulotrosin a (1) is a chiral symmetric dimer, and its relative stereostructure was determined by spectroscopic and x-ray crystallographic analysis. rugulotrosin b (2) is a chiral asymmetric dimer isomeric with 1. its structure was determined by spectroscopic analysis with comparison to the co-metabolite 1 ... | 2004 | 15104517 |
| weathering of phlogopite by bacillus cereus and acidithiobacillus ferrooxidans. | the purpose of this study was to assess the weathering of finely ground phlogopite, a trioctahedral mica, by placing it in contact with heterotrophic (bacillus cereus) and acidophilic (acidithiobacillus ferrooxidans) cultures. x-ray diffraction analyses of the phlogopite sample before and after 24 weeks of contact in b. cereus cultures revealed a decrease in the characteristic peak intensities of phlogopite, indicating destruction of individual structural planes of the mica. no new solid phase p ... | 2004 | 15105888 |
| investigation of metal ion binding in phosphonoacetaldehyde hydrolase identifies sequence markers for metal-activated enzymes of the had enzyme superfamily. | the 2-haloalkanoic acid dehalogenase (had) family, which contains both carbon and phosphoryl transferases, is one of the largest known enzyme superfamilies. had members conserve an alpha,beta-core domain that frames the four-loop active-site platform. each loop contributes one or more catalytic groups, which function in mediating the core chemistry (i.e., group transfer). in this paper, we provide evidence that the number of carboxylate residues on loop 4 and their positions (stations) on the lo ... | 2004 | 15109258 |
| use of novel pcr primers specific to the genes of staphylococcal enterotoxin g, h, i for the survey of staphylococcus aureus strains isolated from food-poisoning cases and food samples in taiwan. | data regarding the incidence of the newly found enterotoxigenic staphylococcus aureus strains in food poisoning cases and in food samples were to date not available in taiwan. in this study, pcr primers specific for the detection of seg, h and i genes, i.e., seg, seh and sei, were used for the assay of 55 human isolates of s. aureus negative to the classical enterotoxins (sea-->see) detection. these isolates were from the fecal specimens of the patients suffering from food poisoning outbreaks. o ... | 2004 | 15109796 |
| inactivation of bacillus cereus spores in milk by mild pressure and heat treatments. | the objective of this work was to study the germination and subsequent inactivation of bacillus cereus spores in milk by mild hydrostatic pressure treatment. in an introductory experiment with strain lmg6910 treated at 40 degrees c for 30 min at 0, 100, 300 and 600 mpa, germination levels were 1.5 to 3 logs higher in milk than in 100 mm potassium phosphate buffer (ph 6.7). the effects of pressure and germination-inducing components present in the milk on spore germination were synergistic. more ... | 2004 | 15109800 |
| the antibacterial innate immune response by the mosquito aedes aegypti is mediated by hemocytes and independent of gram type and pathogenicity. | previous mosquito studies showed that the hemocyte-mediated innate immune response against gram- escherichia coli is phagocytosis, but against gram+ micrococcus sp., is melanization. we examined the immune responses mounted by aedes aegypti towards gram- enterobacter cloacae, klebsiella pneumoniae, pseudomonas aeruginosa, and salmonella typhimurium, and gram+ bacillus cereus, enterococcus faecalis, staphylococcus aureus, and staphylococcus epidermidis. using light microscopy, electron microscopy ... | 2004 | 15109959 |
| cloning and expression of a putative transferrin cdna of the spruce budworm, choristoneura fumiferana. | a spruce budworm (choristoneura fumiferana) transferrin cdna (cftf) was isolated and cloned from a cdna library that was constructed using mrna from fifth to sixth instar larvae. cftf cdna encoded a predicted protein of 681 amino acids with a molecular mass of approximately 76 kda. cftf shared 72% and 74% identities at the amino acid level with transferrins of manduca sexta and bombyx mori, respectively. like other transferrins, cftf retains most of the n-terminal, iron-binding amino acid residu ... | 2004 | 15110871 |
| synthesis and biological evaluation of oxindoles and benzimidazolinones derivatives. | the synthesis of new oxindoles and benzimidazolinones derivatives bearing a sugar residue on the aromatic nitrogen is described. the presence of the glycoside moiety should enhance the solubility of these heterocyclic compounds and/or improve the interaction with the active site of the biological targets. the inhibitory activities of these new compounds toward five kinases were examined: kdr (vegfr-2), fgfr-1, pdgfr-beta, egfr and tie 2. furthermore, the antibacterial activities of the prepared ... | 2004 | 15110971 |
| two additional carbohydrate-binding sites of beta-amylase from bacillus cereus var. mycoides are involved in hydrolysis and raw starch-binding. | in the previous x-ray crystallographic study, it was found that beta-amylase from bacillus cereus var. mycoides has three carbohydrate-binding sites aside from the active site: two (site2 and site3) in domain b and one (site1) in domain c. to investigate the roles of these sites in the catalytic reaction and raw starch-binding, site1 and site2 were mutated. from analyses of the raw starch-binding of wild-type and mutant enzymes, it was found that site1 contributes to the binding affinity to raw- ... | 2004 | 15113834 |
| evaluation of the sanitization effectiveness of a denture-cleaning product on dentures contaminated with known microbial flora. an in vitro study. | to see if dentures contaminated with staphylococcus aureus, pseudomas aeruginosa, bacillus cereus, candida albicans, and herpes simplex virus 1 could be effectively decontaminated by using medical tabs for dentures. | 2004 | 15119677 |
| synergistic interaction in simultaneous exposure to streptomyces californicus and stachybotrys chartarum. | the microbial exposure associated with health complaints in moldy houses consists of a heterogeneous group of components, including both living and dead bacteria, fungi, and their metabolites and active compounds. however, little is known about the interactions between different microbes and their metabolites, although the cytotoxicity and inflammatory potential of certain individual microbes have been reported. in this study, we investigated the inflammatory responses of mouse raw264.7 macropha ... | 2004 | 15121507 |
| bacillus cereus central line infection in an immunocompetent child with hemophilia. | bacillus species are increasingly recognized as pathogens in immunocompromised patients. the authors report a case of bacillus cereus infection of a central line in an immunocompetent patient with hemophilia, which required line removal for complete cure. | 2004 | 15125613 |
| an extracytoplasmic-function sigma factor is involved in a pathway controlling beta-exotoxin i production in bacillus thuringiensis subsp. thuringiensis strain 407-1. | beta-exotoxin i is an insecticidal nucleotide analogue secreted by various bacillus thuringiensis strains. in this report, we describe the characterization and transcriptional analysis of a gene cluster, designated sigw-ecfx-ecfy, that is essential for beta-exotoxin i production in b. thuringiensis subsp. thuringiensis strain 407-1. in this strain, the disruption of the sigw cluster resulted in nontoxic culture supernatants. sigw encodes a protein of 177 residues that is 97 and 94% identical to ... | 2004 | 15126472 |
| growth promotion of red pepper plug seedlings and the production of gibberellins by bacillus cereus, bacillus macroides and bacillus pumilus. | the growth of red pepper plug seedlings was promoted by bacillus cereus mj-1, b. macroides cj-29, and b. pumilus cj-69 isolated from the rhizosphere. gibberellins (gas), a well-known plant growth-promoting hormone, were detected in the culture broth of their rhizobacteria. among the gas, the contents of ga1, ga3, ga4, and ga7, physiologically active gas, were comparatively higher than those of others, suggesting that the growth promoting effect was originated from the gas. this isthe first repor ... | 2004 | 15127789 |
| genome update: rrnas in sequenced microbial genomes. | 2004 | 15133066 | |
| strains of bacillus cereus vary in the phenotypic adaptation of their membrane lipid composition in response to low water activity, reduced temperature and growth in rice starch. | the phenotypic adaptation of membrane lipids in seven strains of the food-poisoning bacterium bacillus cereus, isolated from bangladeshi rice, is reported in relation to their ability to grow under conditions of low water activity (a(w)), reduced temperature and the presence of soluble rice starch. the strains have different membrane phospholipid head-group and fatty acyl compositions, and they display individual differences in their responses to both low a(w) and reduced temperature. the extent ... | 2004 | 15133101 |
| strength in diversity. | 2004 | 15134078 | |
| attenuation regulation of amino acid biosynthetic operons in proteobacteria: comparative genomics analysis. | candidate attenuators were identified that regulate operons responsible for biosynthesis of branched amino acids, histidine, threonine, tryptophan, and phenylalanine in gamma- and alpha-proteobacteria, and in some cases in low-gc gram-positive bacteria, thermotogales and bacteroidetes/chlorobi. this allowed us not only to describe the evolutionary dynamics of regulation by attenuation of transcription, but also to annotate a number of hypothetical genes. in particular, orthologs of ygea of esche ... | 2004 | 15135544 |
| detection of bacterial dna in painful degenerated spinal discs in patients without signs of clinical infection. | a local inflammatory and potentially painful response, of which the ultimate cause is unknown, has been described in nervous tissues in contact with degenerated disc material in patients with low back and leg pain. with the rationale that a possible cause of such inflammation could be bacterial infection, we utilized pcr (polymerase chain reaction) amplification of the 16s rrna (ribosomal rna) gene followed by gene sequencing, to investigate whether bacterial dna might be detected in the degener ... | 2004 | 15138861 |
| [techniques of diseases, insect pests and weeds control and their efficacy in bio-rational rice production]. | studies on the efficacy of bio-rational pesticides and agricultural methods against the chief diseases, insect pests and weeds of rice showed that the efficacy of the mixtures of jingangmycin and bacillus-cereus, and jingangmycin and polyoxin against rice sheath blight were 75.16%-94.27% after sprayed once at the tiller and boot end stages of rice, respectively, and better than that of chemical fungicide triadimefon. the efficacy of kasugamycin and blasticidin was 50.54%-72.67% on rice leaf blas ... | 2004 | 15139200 |
| structural determinants of substrate binding to bacillus cereus metallo-beta-lactamase. | binding and hydrolysis of the beta-lactams cefotaxime, cephapirin, imipenem, and benzylpenicillin by the metallo-beta-lactamase from bacillus cereus were studied by presteady state kinetic measurements. in all cases, the substrate was unmodified in the most populated reaction intermediate, and no chemically modified substrate species accumulated to a detectable amount. the cephalosporins tested showed similar formation rate constants for this intermediate, and they differed mostly in their decay ... | 2004 | 15140877 |
| venom from the pupal endoparasitoid, pimpla hypochondriaca, increases the susceptibility of larval lacanobia oleracea to the entomopathogens bacillus cereus and beauveria bassiana. | cellular immune responses in insects protect them against parasites and pathogens that enter their hemocoel. venom from the solitary pupal endoparasitoid, pimpla hypochondriaca, has previously been shown to suppress certain key, cell-mediated immune responses of lacanobia oleracea. experiments were performed to determine if l. oleracea larvae injected with p. hypochondriaca venom would be more susceptible to bacillus cereus, or beauveria bassiana, when these microorganisms were subsequently inje ... | 2004 | 15145247 |