Publications

characterization and isolation of structural polypeptides in haemagglutinating encephalomyelitis virus.	haemagglutinating encephalomyelitis virus (hev), a member of the coronavirus family, was purified and analysed by sds-polyacrylamide gel electrophoresis. it was shown to contain eight polypeptides, seven of which were glycosylated. they had apparent mol. wt. of 180,000 (gp 180), 130,0000 (gp 130), 120,000 (gp 120) 76,000 (gp 76), 64,000 (vp 64), 54,000 (gp 54), 32,000 (gp 32) and 31,000 (gp 31). electrophoresis of virus samples dissociated under varying conditions showed that gp 54 and gp 120 co ...	1980	7381432
serological relation between calf diarrhea coronavirus and hemagglutinating encephalomyelitis virus.	neutralizing (nt) and hemagglutination-inhibiting (hi) antibodies to calf diarrhea coronavirus (cdcv) and hemagglutinating encephalomyelitis virus of swine (hev) (strain 67n) were detected in high proportions of normal adult cattle and pigs in japan. since comparison of nt and hi titers in the serum samples suggested in antigenic difference between the viruses, cross nt and hi tests of these viruses were carried out with antisera raised in rabbits. the homologous nt titers were markedly higher t ...	1980	6159873
a seroepizootiologic study of five viruses in a swine-evaluation station.	this serologic study was done to gain information on the spread, maintenance, and effect upon performance of five porcine viruses. blood samples were taken from two groups of 8- to 11-week-old pigs from a large number of indiana swine herds in a performance-testing station 1 week after entry, 7 weeks after entry (one group only), and at slaughter. the sera were tested by indirect fluorescent antibody tests for antibodies to transmissible gastroenteritis virus (tgev), swine influenza virus (siv), ...	1979	230762
effect of sulphydryl reagents on the biological activities, polypeptide composition and morphology of haemagglutinating encephalomyelitis virus.	the effect of sulphydryl reagents on haemagglutinating encephalomyelitis virus (hev), a coronavirus of pigs, was investigated. using increasing concentrations of dithiothreitol (dtt), 50% of the virus infectivity and haemagglutination (ha) activity could be removed by i.5 mm and 4 to 5 mm respectively. the effect of dtt concentrations on the polypeptide composition was also examined. of the three external glycoproteins gp 125 was found to be the most susceptible, 50% being removed by incubation ...	1978	567674
antigenic relationship of the feline infections peritonitis virus to coronaviruses of other species.	utilizing the direct and indirect fluorescent antibody procedure, the antigenic relationship of the feline infectious peritonitis virus (fipv) to 7 other human and animal coronaviruses was studied. fipv was found to be closely related to transmissible gastroenteritis virus (tgev) of swine. transmissible gastroenteritis virus and fipv were in turn antigenically related to human coronavirus 229e (hcv-229e) and canine coronavirus (ccv). an interesting finding in the study was that the 8 coronavirus ...	1978	81044
an electron microscopic study of experimentally-induced hev encephalitis.	a gnobiotic piglet, was inoculated intracerebrally with hemagglutinating encephalomyelitis virus (strain vw572). mononuclear cells formed vascular cuffs and were disseminated in the brain parenchyma. a few neurons were surrounded by the same kind of cells. virus particles morphologically similar to coronavirus particles were found in the cytoplasm of both chromatolytic light neurons and hyperchromic dark neurons. the particles were in vesicles of distended endoplasmic reticulum and in the hypert ...	1978	204096
experimental infection of chickens with hemorrhagic enteritis virus.	chickens were experimentally infected with hemorrhagic enteritis virus, causing lesions similar to those observed in turkeys. lesions were induced by intraperitoneal and oral infection. virus particles with the morphology of adenovirus were demonstrated in the spleens of infected chickens. splenic extracts from infected chickens caused lesions of hemorrhagic enteritis in turkeys. recovery from the infection in chickens was followed by the production of antibodies to hev, demonstrated by agar-gel ...	1978	206247
antigenic relationship between human coronavirus strain oc 43 and hemagglutinating encephalomyelitis virus strain 67n of swine: antibody responses in human and animal sera.	hemagglutinating encephalomyelitis virus of swine (hev) was adapted to growth in suckling mouse brain. electron micrographs of hev-infected suckling mouse brain, prepared by negative staining and thin-section techniques, exhibited typical morphological characteristics shared with other members of the coronaviridae. the adaptation of hev to suckling mouse brain facilitated serologic testing by the use of common host reagents and compatible animal systems. with hemagglutination inhibition, complem ...	1977	65439
porcine parvovirus: frequency of naturally occurring transplacental infection and viral contamination of fetal porcine kidney cell cultures.	the frequency of naturally occurring transplacental infection of swine with porcine parvovirus (ppv) and one of the possible consequences of such infection--the presence of ppv in cell cultures prepared from fetal tissues--were investigated. transplacental infection was indicated by the presence of high titers of hemagglutination inhibiting (hi) antibody for ppv in serums of 0-day-old, hysterectomy-derived, colostrum-deprived pigs of 3 of 82 litters. all letters were farm-raised dams. moreover, ...	1975	163603
incidence of antibody for hemagglutinating encephalomyelitis virus in serums from swine in the united states.	serums prepared from blood collected from swine in arizona, florida, georgia, hawaii, iowa, maine, new hampshire, oregon, vermont, and washington were examined by the hemagglutination-inhibition (hi) test for antibody to hemagglutinating encephalomyelitis virus (hev). antibody was detected in serums from swine in each of the states except maine, new hampshire, and vermont. the frequency of antibody ranged from 10.9% (6 of 55) with serums from swine in oregon, to 98.6% (71 of 72) with serums from ...	1975	807134
[hemagglutinating encephalomyelitis virus (hev) infections in swine. serologic indication for infectional occurrence in denmark].	in 1956 a disease of nursing pigs previously unreported in denmark, was observed in several swine herds. the disease syndrome appeared identical with that later described by roe and alexander in 1958 and by s. f. cartwright and m. lucas in 1968. since 1956 there have been no reports of such outbreaks in danish herds. to investigate whether the virus is still present, a survey was carried out to detect neutralizing antibodies in sera from a number of boars using a micro technique. in 144 sera fro ...	1975	1144016
studies on the hemagglutination phenomenon of hemagglutinating encephalomyelitis virus (hev) of pigs.	hemagglutinating encephalomyelitis virus (hev), unlike other coronaviruses, readily agglutinates a variety of red blood cells. the reaction differs from that of the myxovirus/paramyxovirus groups in several respects. unlike the myxoviruses, hev agglutinated red cells treated with receptor destroying enzyme. no neuraminidase activity was demonstrated with hev and there was no apparent elution of the virus from agglutinated cells. mucins and animal sera inhibited the agglutination reaction markedl ...	1972	4263916
studies on the hepato-encephalomyelitis virus (hev).		1954	13208494
serologic study of pig-associated viral zoonoses in laos.	we conducted a serologic survey of four high-priority pig-associated viral zoonoses, japanese encephalitis virus (jev), hepatitis e virus (hev), nipah virus (niv), and swine influenza virus (siv), in laos. we collected blood from pigs at slaughter during may 2008-january 2009 in four northern provinces. japanese encephalitis virus hemagglutination inhibition seroprevalence was 74.7% (95% confidence interval [ci] = 71.5-77.9%), jev igm seroprevalence was 2.3% (95% ci = 1.2-3.2%), and hev seroprev ...	0	22665622
hepatitis e virus in pork production chain in czech republic, italy, and spain, 2010.	we evaluated the prevalence of hepatitis e virus (hev) in the pork production chain in czech republic, italy, and spain during 2010. a total of 337 fecal, liver, and meat samples from animals at slaughterhouses were tested for hev by real-time quantitative pcr. overall, hev was higher in italy (53%) and spain (39%) than in czech republic (7.5%). hev was detected most frequently in feces in italy (41%) and spain (39%) and in liver (5%) and meat (2.5%) in czech republic. of 313 sausages sampled at ...	0	22840221
wildlife reservoir for hepatitis e virus, southwestern france.	pigs are a reservoir for hepatitis e virus (hev). to determine the relative contribution of game to the risk for human hev infection in southwestern france, we tested wildlife samples. hev rna was in 3.3% of wildlife livers, indicating that in this region, eating game meat is as risky as eating pork.	0	26079541
hepatitis e virus in yellow cattle, shandong, eastern china.		0	27869603