Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| recombinant respiratory syncytial virus bearing a deletion of either the ns2 or sh gene is attenuated in chimpanzees. | the ns2 and sh genes of respiratory syncytial virus (rsv) have been separately deleted from a recombinant wild-type rsv strain, a2 (m. n. teng and p. l. collins, j. virol. 73:466-473, 1998; a. bukreyev et al., j. virol. 71:8973-8982, 1997; and this study). the resulting viruses, designated ra2deltans2 and ra2deltash, were administered to chimpanzees to evaluate their levels of attenuation and immunogenicity. recombinant virus ra2deltans2 replicated to moderate levels in the upper respiratory tra ... | 1999 | 10074199 |
| antiviral activity in argentine medicinal plants. | in an ethnopharmacological screening of selected medicinal plants used in argentina for the treatment of infectious diseases, aqueous extracts of five species were assayed in vitro to detect antiviral activity against herpes simplex virus type 1 (hsv-1), respiratory syncytial virus (rsv) and adenovirus serotype 7 (adv-7). polygonum punctatum, lithraea molleoides, sebastiania brasiliensis and sebastiania klotzschiana but not myrcianthes cisplatensis showed in vitro antiherpetic activity with 50% ... | 1999 | 10075125 |
| respiratory syncytial virus g glycoprotein expressed using the semliki forest virus replicon is biologically active. | the respiratory syncytial virus (rsv) g glycoprotein mediates attachment of rsv to cells via an unknown receptor. to study g glycoprotein function we have cloned two variants of the rsv g gene into a semliki forest virus (sfv) expression vector, a full length (rg) and soluble (srg) g glycoprotein variant. by immunofluorescence microscopy, rg was found to be predominantly membrane associated, while srg was mostly cytoplasmic. the rg (80-85 kda) and srg (75-80 kda) constructs produced heavily glyc ... | 1999 | 10076512 |
| infection with respiratory syncytial virus enhances expression of native receptors for non-pilate neisseria meningitidis on hep-2 cells. | respiratory virus infections have been suggested to be predisposing factors for meningococcal disease. respiratory syncytial virus (rsv) affects young children in the age range at greatest risk of disease caused by neisseria meningitidis. it has been previously shown that glycoprotein g expressed on the surface of rsv-infected hep-2 cells (a human epithelial cell line) contributed to higher levels of binding of meningococci compared with uninfected cells. the aim of the present study was to exam ... | 1999 | 10076908 |
| overexpression of a human potassium channel suppresses cardiac hyperexcitability in rabbit ventricular myocytes. | the high incidence of sudden death in heart failure may reflect abnormalities of repolarization and heightened susceptibility to arrhythmogenic early afterdepolarizations (eads). we hypothesized that overexpression of the human k+ channel herg (human ether-a-go-go-related gene) could enhance repolarization and suppress eads. adult rabbit ventricular myocytes were maintained in primary culture, which suffices to prolong action potentials and predisposes to eads. to achieve efficient gene transfer ... | 1999 | 10079110 |
| stimulus-specific regulation of chemokine expression involves differential activation of the redox-responsive transcription factors ap-1 and nf-kappab. | the promoters of the il-8, mcp-1, and rantes genes contain binding sites for the redox-responsive transcription factors ap-1 and nf-kappab, which have been shown to be important for their expression. in this overview, we present evidence from our laboratories that the stimulus-specific regulation of these chemokines by the reactive oxidant h2o2, the proinflammatory cytokine tnf-alpha, and respiratory syncytial virus (rsv) is mediated in a cell type-specific manner involving different patterns of ... | 1999 | 10080530 |
| protective effects of type i and type ii interferons toward rous sarcoma virus-induced tumors in chickens. | growth of tumors induced by rous sarcoma virus (rsv) is controlled by alleles at the major histocompatibility complex locus in chickens, indicating that immunological host defense mechanisms play a major role. we show here that the resistance phenotype of cb regressor chickens can be partially reverted by treating the animals with a monoclonal antibody that neutralizes the major serotype of chicken type i interferon, chifn-alpha. injection of recombinant chifn-alpha into susceptible cc progresso ... | 1999 | 10087229 |
| palivizumab for respiratory syncytial virus prophylaxis. | palivizumab, a humanized monoclonal antibody, has been approved by the fda to prevent severe lower respiratory tract infections caused by rsv in high-risk patients. prophylaxis of rsv infections with palivizumab requires monthly injections (15 mg/kg) during the rsv season. in the impact-rsv study, hospitalizations resulting from rsv decreased by 55% in the palivizumab treatment group. palivizumab has also been shown to decrease the number of days with moderate or severe rsv infection, with an in ... | 1999 | 10690084 |
| integrase-mediated nonviral gene transfection with enhanced integration efficiency. | retroviruses efficiently integrate their genome into the host chromosome. two elements of the retrovirus genome are needed for the integration: long terminal repeats (ltrs) and integrase protein. we attempted to incorporate the retrovirus integration machinery in lipid vesicle-mediated gene transfection with the aim of achieving efficient stable transfection in a nonviral gene transfection system. a dna fragment, in which a neomycin-resistant gene was flanked between partial ltr sequences derive ... | 1999 | 16232645 |
| [mite-induced interleukin-5 and interleukin-4 expressions in peripheral blood mononuclear cells from asthmatic children with respiratory syncytial virus infection]. | to investigate the effects of respiratory syncytial virus(rsv) infection on mite-induced interleukin-5 and interleukin-4 expressions in cultures of pbmc from asthmatic children and their correlation with asthmatic symptoms. | 1999 | 11775916 |
| [nutritional status and clinical evolution of hospitalized chilean infants with infection by respiratory syncytial virus (rsv)]. | respiratory syncycial virus (rsv) is the first cause of acute lower respiratory tract infection in chilean infants. a significant impact of nutrition on clinical course of these infections has been described. in order to analyze the association between nutritional status (ns) and clinical course of infants hospitalized with acute lower respiratory tract infection due to rsv, 130 infants (mean age 5.8 +/- 4.9 m) without chronic diseases, admitted to hospital with confirmed rsv infection, were stu ... | 1999 | 10883296 |
| respiratory syncytial virus infection of human respiratory epithelial cells enhances both muscarinic and beta2-adrenergic receptor gene expression. | the possible changes in transcriptional activities of the m1 muscarinic acetylcholine receptor (machr) and beta2-adrenergic receptor (ar) genes in respiratory syncytial virus (rsv)-infected human type 2 alveolar epithelial cells (a549 cells) were analyzed semiquantitatively by reverse transcription-polymerase chain reaction (rt-pcr). rsv enhanced m1 machr gene expression significantly at 4 hrs post infection (p.i.), and this enhancement persisted until 10 hrs, after peaking at 7 hrs. beta2-ar ge ... | 1999 | 10749375 |
| effect of intercistronic length on internal ribosome entry site (ires) efficiency in bicistronic mrna. | specific structures found in the mrna of picornavirus are known to allow a cap-independent translation. these structures, named internal ribosome entry sites (ires), are also able to favor translation of the second cistron in bicistronic mrnas. their mechanism of action is not well understood. in the present study, two iress have been used: the ires from poliovirus and a newly discovered ires (sur) composed of the 5' p untranslated sequence from sv40 early genes, the r structure, and a small par ... | 1999 | 10947079 |
| [cloning and sequencing of cdna from g protein gene of subgroup b respiratory syncytial virus strain isolated in china]. | the nucleotide sequence of the g protein gene of respiratory syncytial virus (rsv) cc169 strain isolated from china that has been identified as subgroup b with monoclonal antibodies, was determined from cdna that had been amplified by rt-pcr and cloned into ptz18r plasmid vector. the homology of nucleotide was 94% as compared with g protein cdna of a rsv prototype strain (ch18537). deduced amino acid identity of g protein was 89.4%. the amino acid changes were only in the extracellular part of t ... | 1999 | 12555397 |
| [cloning and sequencing analysis of rice stripe virus genome segment 4 of chinese isolate y]. | the cdna fragment covering full-length sequence of rstv rna4 of yunnan isolate in china was obtained by rt-pcr. the pcr-derived fragment was then cloned into vector pcrii. the cloned cdna was sequenced. comparison of the nucleotide and deduced amino acid sequences with those of the japanese isolate t was made. the results showed that at the nucleotides level, vorf, vcorf and the intergenic region had 94.9%, 94.1% and 86.1% identity respectively, the 5'-untranslational region was exactly the same ... | 1999 | 12555399 |
| [molecular epidemiology of respiratory syncytial virus infection in infants]. | to search for the molecular epidemiology of respiratory syncytial virus (rsv) infection in infants. | 1999 | 12759984 |
| analysis of antibiotic use and misuse in children hospitalized with rsv infection. | to determine the extent of, rationale for and acquisition cost of antibiotic use in a cohort of children with lower respiratory tract infection (lri) secondary to the respiratory syncytial virus (rsv). | 1999 | 20212965 |
| antiviral immunotherapy: a review of current status. | unselected intramuscular (im) and intravenous (iv) immunoglobulins, as well as virus-specific hyperimmune globulins, occupy important roles as immunotherapy for viral infections. standard im immunoglobulins may be utilised in selected, susceptible patients for the prevention of hepatitis a and measles. hyperimmune globulins to varicella zoster virus (vzv), hepatitis b virus and rabies have established indications for use as post-exposure prophylaxis. cytomegalovirus (cmv) hyperimmune globulin ha ... | 1998 | 18020581 |
| the therapeutic potential of monoclonal antibodies against respiratory syncytial virus. | attempts to develop a vaccine against respiratory syncytial virus (rsv), the major cause of lower respiratory tract disease in infants and young children, have been unsuccessful. passive immunisation with antibody to rsv has been found to be an effective alternative method for prophylaxis. the product currently in use for rsv passive immunisation, a preparation of purified human igg containing virus-neutralising activity, requires monthly iv. infusions. monoclonal antibodies (mabs) are currently ... | 1998 | 15992030 |
| rsv-associated hospitalizations in alaska native infants. | retrospective reviews for 1986-1992 suggested that alaska native children experience high rates of respiratory syncytial virus (rsv)-associated hospitalization; however, the epidemiology of rsv infections has been poorly characterized. | 1998 | 10093285 |
| a review of respiratory syncytial virus infection in infants and children. | by the age of 2, nearly every child becomes infected with the respiratory syncytial virus (rsv), the most common cause of lower respiratory tract infections (rtis) in infants and children. yearly epidemics occur from october to may. most infections are mild, producing nothing more than a cold, and can be managed at home. some cases, however, are more severe, leading to bronchiolitis, pneumonitis, pneumonia, and even death. | 1998 | 10030209 |
| autocrine regulation of interleukin-8 by interleukin-1alpha in respiratory syncytial virus-infected pulmonary epithelial cells in vitro. | respiratory epithelial cells infected with respiratory syncytial virus (rsv) produce interleukin-8 (il-8); however, the mechanisms of rsv-induced regulation of il-8 are poorly understood. in the present study, the regulation of il-8 by rsv was evaluated using pulmonary type ii-like epithelials (a549). live purified rsv (prsv) induced a significant increase in il-8 after 8 hr of exposure, while conditioned supernatants from prsv-infected a549 cells (crsv) induced il-8 production in fresh a549 cul ... | 1998 | 9893037 |
| distribution, persistency, toxicity, and lack of replication of an e1a-deficient adenoviral vector after intracardiac delivery in the cotton rat. | adenoviral vectors were inoculated via intracardiac injection into 5- to 1o-week-old cotton rats (sigmodon hispidus) to evaluate the effects of systemic delivery. cotton rats were chosen as a model because they are semipermissive to the replication of human adenoviruses. the vector used was adv.rsv-tk, a replication-deficient adenovirus with a herpes simplex virus thymidine kinase gene inserted in the e1 region. vector doses were 3 x 10(8), 3 x 10(9), and 3 x 10(10) viral particles per animal wi ... | 1998 | 9917091 |
| respiratory viruses in children attending a major referral centre in saudi arabia. | viral respiratory infections are common causes of illness in infants and children. examination of clinical specimens submitted for diagnosis during a 3-year period (august 1993-july 1996) at king faisal specialist hospital and research center (kfsh & rc) in riyadh revealed a wide spectrum of diseases associated with the isolation of five respiratory viruses. severity of disease ranged from mild upper respiratory illness to threatening lower respiratory illnesses including bronchiolitis and pneum ... | 1998 | 9924568 |
| usefulness of bronchoalveolar lavage for diagnosis of acute and persistent respiratory syncytial virus lung infections in guinea pigs. | to investigate whether bronchoalveolar lavage (bal) fluid specimens can be used to diagnose acute and persistent respiratory syncytial virus (rsv) lung infections in guinea pigs, we tested bal fluid and lung tissue specimens for evidence of viral infection, and compared bal cytology between infected and uninfected animals. rsv-inoculated guinea pigs were studied during acute bronchiolitis (days 3 and 7 postinoculation), convalescence (day 14 postinoculation), and persistent infection (days 28 an ... | 1998 | 9888214 |
| genes of chicken mhc regulate the adherence activity of blood monocytes in rous sarcomas progressing and regressing lines. | the influence of the chicken major histocompatibility (b) complex (mhc) on the adherence potential of monocyte-derived macrophages was examined using the congenic chicken lines cb and cc. these lines represent well-defined genetic models for the study of resistance (cb) or susceptibility (cc) to the progressive growth of rous sarcomas. using a monoclonal antibody specific for chicken monocytes/macrophages, cb and cc chickens were shown by flow cytometry analyses to have similar proportions of pe ... | 1998 | 9860187 |
| update: respiratory syncytial virus activity--united states, 1997-98 season. | respiratory syncytial virus (rsv) is the single most important cause of serious lower respiratory tract disease in infants and young children worldwide. in temperate climates, infections primarily occur during yearly outbreaks that usually peak during the winter months. rsv activity in the united states is monitored by the national respiratory and enteric virus surveillance system (nrevss), a voluntary, laboratory-based system. this report summarizes trends for rsv reported to nrevss from july 1 ... | 1998 | 9869076 |
| [severity of respiratory syncytial virus infection influenced by clinical risk factors and subtype a and b in hospitalized children]. | since it is possible to identify the subgroups of rsv, a-subtype and b-subtype, there are findings indicating that the subtype may influence severity of rsv infection. our study was designed to assess the hypothesis that a-subtype infections were more severe than b-subtype infections among hospitalized children. | 1998 | 9871899 |
| permissiveness of guinea pig alveolar macrophage subpopulations to acute respiratory syncytial virus infection in vitro. | alveolar macrophages (ams) are targets for respiratory syncytial virus (rsv) infection in vivo and in vitro. however, only a minority of ams are permissive to acute rsv infection in vitro, and it is unknown whether this permissiveness may be related to the degree of cellular maturation that is achieved in vivo. | 1998 | 9872206 |
| detection of bordetella pertussis and respiratory synctial virus in air samples from hospital rooms. | to evaluate the distribution of bordetella pertussis and respiratory syncytial virus (rsv) in the hospital setting. | 1998 | 9872529 |
| role of cellular actin in the gene expression and morphogenesis of human respiratory syncytial virus. | cytoskeletal protein actin and nonactin cellular proteins were essential for human respiratory syncytial virus (rsv) gene expression. in vitro, specific antibodies against actin inhibited rsv transcription, whereas antibodies against other cytoskeletal proteins had little or no effect. affinity purified cellular actin or bacterially expressed recombinant actin activated rsv transcription. however, optimal transcription required additional cellular protein(s) that appeared to function as accessor ... | 1998 | 9875324 |
| approaches to antiviral chemotherapy for acute respiratory infections. | the causative agents of acute respiratory infections (ari) in infants and children are mostly thought to be viruses. some ari in adult patients may be caused by bacteria but most often the causes are virus infections. when ari affect immunocompromised patients or the elderly the mortality rates are significantly higher than in immunocompetent individuals. many types of viruses cause ari. among them, influenza viruses a and b and respiratory syncytial virus (rsv) are thought to be the most import ... | 1998 | 9875381 |
| severity of respiratory syncytial virus disease related to type and genotype of virus and to cytokine values in nasopharyngeal secretions. | investigations concerning the severity of respiratory syncytial virus (rsv) disease as related to (1) rsv type and genotype determined respectively by pcr and restriction enzyme analysis and (2) interleukin 6 (il-6) and tumor necrosis factor alpha (tnf-alpha) values in samples of nasopharyngeal secretion (nps) have not been previously reported. | 1998 | 9877358 |
| monoclonal antibody-resistant mutants selected with a respiratory syncytial virus-neutralizing human antibody fab fragment (fab 19) define a unique epitope on the fusion (f) glycoprotein. | a recombinant human antibody fragment, designated rsv fab 19, efficiently neutralizes respiratory syncytial virus (rsv). here we report the results of our sequence analysis of antibody escape mutants that identified f glycoprotein amino acids critical for binding of human or murine rsv f-neutralizing antibodies. | 1998 | 9878616 |
| epidemiology of respiratory syncytial virus infection requiring hospitalization in east denmark. | prophylaxis against infection caused by respiratory syncytial virus (rsv) with high titered rsv immunoglobulin or humanized antibody may soon be available in europe. | 1998 | 9849981 |
| safety, tolerance and pharmacokinetics of a humanized monoclonal antibody to respiratory syncytial virus in premature infants and infants with bronchopulmonary dysplasia. medi-493 study group. | respiratory syncytial virus (rsv) is the most common cause of lower respiratory tract infection in infants. medi-493 (palivizumab) is a humanized monoclonal antibody to the fusion protein of rsv and is active in animal models for prevention of pulmonary rsv replication. | 1998 | 9493805 |
| synthesis of human erythropoietin in vivo in the oviduct of laying hens by localized in vivo gene transfer using electroporation. | in order to synthesize human erythropoietin protein in the oviduct of laying hens, localized in vivo gene transfer was attempted by using electroporation. in experiment 1, transcriptional activities were compared by using four viral and cellular promoters, i.e., the 1.35-kbp long ovalbumin promoter, sv40 early promoter, rous sarcoma virus long terminal repeat (rsv ltr), and the miw promoter, which is a hybrid of rsv ltr and chicken beta-actin promoter. these promoters were fused immediately upst ... | 1998 | 9495497 |
| ventricular arrhythmia associated with respiratory syncytial viral infection. | respiratory syncytial virus (rsv) is a common cause of lower respiratory infections in infants. cardiac arrhythmias are an uncommon complication in infants with rsv bronchiolitis. presumably, the arrhythmias may be secondary to rsv pericarditis, myocarditis, or some other infection of the heart. we report on a patient with rsv bronchiolitis who had clinical myocarditis complicated by ventricular tachycardia. | 1998 | 9770583 |
| respiratory syncytial virus (rsv) disease and prospects for its control. | respiratory syncytial virus (rsv) is a major virus pathogen of infants and young children, an important cause of disease in adults and is responsible for a significant amount of excess morbidity and mortality in the elderly. it also can be devastating in immunosuppressed populations. vaccines are being developed, but none are currently licensed. moreover, even if one or more are approved, they may not be suitable for some populations vulnerable to rsv (e.g. very young infants and the immunosuppr ... | 1998 | 9806484 |
| primer trna(trp) of rsv-transformed or rav-1-infected cells up-regulates the antiribosomal activity of gelonin. | some ribosome-inactivating proteins (rips) with rna-n-glycosidase activity on 28s rrna require, for maximal inactivation of ribosomes, the presence of trna. trna(trp) specifically up-regulates gelonin, the rip from gelonium multiflorum. the same trna is the primer of the reverse transcriptase of rous sarcoma virus (rsv) and of its mutant (rav-1) which lacks the src gene. here we demonstrate that gelonin is more active in inhibiting endogenous protein synthesis by lysates of rsv-transformed or ra ... | 1998 | 9810463 |
| the genetic control of susceptibility to subgroup d rna tumour virus infection in commercial breeds of chickens. | the genetic control of resistance to rsv (rav-50) in chick embryos inoculated via the chorioallantoic membrane route was studied in three breeds of chickens. the relative susceptibility to subgroup d infection was 0.01, 0.02 and 0.003 in broilers, white leghorns and bantam x australorp crosses, respectively. the distribution pattern of putative dr gene frequencies were 0.80, 0.68 and 0.90, respectively, in the same three breeds. liver tumours resulting from the virus infection were observed in a ... | 1998 | 9810639 |
| secondary structure and mutational analysis of the ribosomal frameshift signal of rous sarcoma virus. | expression of the gag-pol polyprotein of rous sarcoma virus (rsv) requires a -1 ribosomal frameshifting event at the overlap region of the gag and pol open reading frames. the signal for frameshifting is composed of two essential mrna elements; a slippery sequence (aaauuua) where the ribosome changes reading frame, and a stimulatory rna structure located immediately downstream. this rna is predicted to be a complex stem-loop but may also form an rna pseudoknot. we have investigated the structure ... | 1998 | 9813113 |
| detection and quantitation of human respiratory syncytial virus (rsv) using minigenome cdna and a sindbis virus replicon: a prototype assay for negative-strand rna viruses. | we describe here a novel approach for detecting and quantitating human respiratory syncytial virus (rsv) based on expression of a reporter gene from an rsv minigenome. bhk cells were cytoplasmically transformed with a noncytopathic sindbis virus replicon expressing t7 rna polymerase. these cells were then cotransfected with t7 expression plasmids that contain the cdna of an rsv minigenome and the genes for rsv nucleocapsid proteins n, p, and l. the minigenome contains a reporter gene such as lac ... | 1998 | 9813215 |
| recombinant human respiratory syncytial virus (rsv) from cdna and construction of subgroup a and b chimeric rsv. | infectious human respiratory syncytial virus (rsv) was produced from a cdna clone that contains 15,222 nucleotides of rsv genome derived from the a2 strain of subgroup a. recovery of infectious rsv from cdna required cotransfection of only three expression plasmids encoding the nucleoprotein (n), the phosphoprotein (p), and the major polymerase protein (l). inclusion of the m2-1 plasmid was not required in the transfection reaction and if included did not significantly increase the rescue effici ... | 1998 | 9813216 |
| shedding of l-selectin and pecam-1 and upregulation of mac-1 and icam-1 on neutrophils in rsv bronchiolitis. | bronchiolitis is characterized histologically by epithelial necrosis and peribronchial infiltration of leukocytes, with a high percentage of neutrophils in the airways. we investigated the expression of adhesion molecules (cd11a, cd11b, cd18, cd31, cd54, and cd62l) on neutrophils from nasopharyngeal aspirates (npas) and peripheral blood (pb) of infants with respiratory syncytial virus (rsv)-induced bronchiolitis. the expression of cd31 and cd62l on neutrophils from npas is decreased and the expr ... | 1998 | 9815117 |
| reduction of respiratory syncytial virus (rsv) in tracheal aspirates in intubated infants by use of humanized monoclonal antibody to rsv f protein. | thirty-five children <2 years of age mechanically ventilated for respiratory syncytial virus (rsv) infection were randomized to receive an intravenous infusion of 15 mg/kg medi-493 or placebo. rsv concentration was measured in tracheal secretions by plaque assay before and at 24-h intervals after treatment. the reduction in tracheal rsv concentration from day 0 to day 1 (-1.7+/-0.28 vs. -0. 6+/-0.21 log10 pfu/ml; p=.004) and from day 0 to day 2 (-2.5+/-0.26 vs. -1.0+/-0.41 log10 pfu/ml; p=.012) ... | 1998 | 9815203 |
| atypical pulmonary eosinophilia is mediated by a specific amino acid sequence of the attachment (g) protein of respiratory syncytial virus. | we analyzed the immune responses evoked by a series of overlapping peptides to better understand the molecular basis for respiratory syncytial virus (rsv) g protein-induced eosinophilia in balb/c mice. in vitro stimulation of spleen cells from natural g protein-primed mice showed dominant proliferative and cytokine (interferon [ifn]-gamma and interleukin [il]-5) responses to a peptide encompassing amino acids 184-198. mice vaccinated with peptide 184- 198 conjugated to keyhole limpet hemocyanin ... | 1998 | 9815273 |
| treatment of respiratory failure with inhaled nitric oxide and high- frequency ventilation in an infant with respiratory syncytial virus pneumonia and bronchopulmonary dysplasia. | in a 7-month-old infant with bronchopulmonary dysplasia and respiratory syncytial virus (rsv) pneumonia, we have shown an additive effect of high-frequency ventilation (hfv) and inhaled nitric oxide (ino) in terms of improved oxygenation and the avoidance of extracorporeal membrane oxygenation. apparently, the combined therapy of hfv and ino is superior to either therapeutic modality alone in the treatment of hypoxemic respiratory failure due to rsv pneumonia. the mechanism of increased lung exp ... | 1998 | 9817963 |
| intracellular ifn-gamma expression in natural killer cells precedes lung cd8+ t cell recruitment during respiratory syncytial virus infection. | natural killer (nk) cells are recruited locally during the initial phases of virus infection and produce cytokines which may affect the subsequent emergence of specific t cells. in this study, cellular responses to primary respiratory syncytial virus (rsv) infection and after vaccination with individual viral proteins were investigated in balb/c mice using the new nk cell antibody, dx5. purified dx5+ cells caused lysis of yac-1 cell targets. dx5+ cells did not express cd8, cd45r or mhc class ii ... | 1998 | 9820134 |
| in vivo suppression of osteosarcoma pulmonary metastasis with intravenous osteocalcin promoter-based toxic gene therapy. | pulmonary metastases are the main cause of death of patients with several types of cancer, including osteosarcoma, renal cell carcinoma, malignant melanoma, and breast cancer. previously, we demonstrated that intralesional injection of the recombinant adenovirus (ad) vector containing the herpes simplex virus thymidine kinase (tk) gene driven by an osteocalcin (oc) promoter (ad-oc-tk) effectively suppressed the growth of osteosarcoma cells in vitro and tumors in vivo in a tumor-specific manner w ... | 1998 | 9824046 |
| respiratory syncytial virus infection in children with acute respiratory infections in zambia. | epidemiological research on respiratory syncytial virus (rsv) infections in children was carried out at the virology laboratory, university teaching hospital (uth), in lusaka, zambia, from january-december 1996. specimens including 736 nasal washings and 2424 throat swabs were collected from children with acute respiratory infections (ari) and tested for rsv by enzyme immunoassay and by virus isolation. rsv was isolated in 62 (4.1%) of 1496 throat swabs collected from march to september and was ... | 1998 | 9825792 |
| evolution of antiviral activity in the ribonuclease a gene superfamily: evidence for a specific interaction between eosinophil-derived neurotoxin (edn/rnase 2) and respiratory syncytial virus. | we have demonstrated that the human eosinophil-derived neurotoxin (edn, rnase 2), a rapidly evolving secretory protein derived from eosinophilic leukocytes, mediates the ribonucleolytic destruction of extracellular virions of the single-stranded rna virus respiratory syncytial virus (rsv). while rnase activity is crucial to antiviral activity, it is clearly not sufficient, as our results suggest that edn has unique structural features apart from rnase activity that are necessary to promote antiv ... | 1998 | 9826755 |
| host genetic determinants of vaccine-induced eosinophilia during respiratory syncytial virus infection. | in balb/c mice, sensitization with the attachment protein (g) of respiratory syncytial virus (rsv) leads to cd4+ t cell-mediated lung eosinophilia during subsequent challenge with rsv. to determine the host genetic influences on this model of lung eosinophilia, we tested 15 different inbred mouse strains. eosinophilia developed in all h-2d (balb/c, dba/2n, and b10.d2), but not in h-2k (cba/ca, cba/j, c3h, balb.k, or b10.br) mouse strains. among h-2b mice, 129 and balb.b developed eosinophilia, w ... | 1998 | 9834108 |
| a primate model of respiratory syncytial virus infection. | to determine whether bonnet monkeys are susceptible to infection and disease due to respiratory syncytial virus (rsv), 4 juvenile bonnet monkeys (macaca radiata) were inoculated with rsv intratracheally and sacrificed at 3, 5, 7 and 9 days post infection. rsv was cultured from pre-autopsy broncheoalveolar lavage fluid from all 4 animals with a peak titre of virus on day 9. serum rsv neutralizing antibody was present by day 7. animals developed tachypnoea and chest retractions by 5th day post inf ... | 1998 | 9838875 |
| costs and benefits of respiratory syncytial virus immunoglobulin to prevent hospitalization for lower respiratory tract illness in very low birth weight infants. | respiratory syncytial virus immunoglobulin intravenous (rsv-igiv) has been shown to reduce the risk of lower respiratory illness (lri) hospitalization in preterm infants and infants with bronchopulmonary dysplasia (bpd). the purpose of this analysis was to estimate the economic costs and benefits of prophylaxis with rsv-igiv in these groups. | 1998 | 9686723 |
| enzyme-linked immunoassay for respiratory syncytial virus is not predictive of bronchiolitis in sudden infant death syndrome. | although respiratory syncytial virus (rsv)-infected infants may present with apnea, the role that rsv plays in sudden infant death syndrome (sids) is speculative. to determine whether rsv is associated with bronchiolitis in these patients, we examined histologic sections of lungs from 41 apparent sids cases and compared the results with those of enzyme-linked immunofluorescent assay (eia) from nasal washings. bronchiolitis was defined by a bronchiolar inflammatory cell infiltrate plus epithelial ... | 1998 | 9688761 |
| pasteurella haemolytica complicated respiratory infections in sheep and goats. | respiratory infections which commonly occur in sheep and goats often result from adverse physical and physiological stress combined with viral and bacterial infections. inevitably, pasteurella haemolytica pneumonia occurs as a result of these interactions. in this review, we present recent advances in research on the complex etiology of pneumonia involving p. haemolytica. initially stress, induced by factors such as heat, overcrowding, exposure to inclement weather, poor ventilation, handling an ... | 1998 | 9689740 |
| heparin-like structures on respiratory syncytial virus are involved in its infectivity in vitro. | addition of heparin to the virus culture inhibited syncytial plaque formation due to respiratory syncytial virus (rsv). moreover, pretreatment of the virus with heparinase or an inhibitor of heparin, protamine, greatly reduced virus infectivity. two anti-heparan sulfate antibodies stained rsv-infected cells, but not noninfected cells, by immunofluorescence. one of the antibodies was capable of neutralizing rsv infection in vitro. these results prove that heparin-like structures identified on rsv ... | 1998 | 9696816 |
| interleukin-8 production by cystic fibrosis nasal epithelial cells after tumor necrosis factor-alpha and respiratory syncytial virus stimulation. | high levels of neutrophils and the neutrophil-attracting chemokine interleukin (il)-8 have been observed in the airways of patients with cystic fibrosis (cf). we hypothesized that cf respiratory epithelium produces excessive amounts of il-8 either at baseline or after stimulation. to test this hypothesis we compared immunoreactive il-8 release by primary nasal epithelial cell (nec) cultures established from young children with or without cf, at several time points after stimulation of cultures w ... | 1998 | 9698592 |
| standardizing the care of bronchiolitis. | to study the effect of an educational intervention on the management of hospitalized infants with bronchiolitis. | 1998 | 9701131 |
| a single nucleotide substitution in the transcription start signal of the m2 gene of respiratory syncytial virus vaccine candidate cpts248/404 is the major determinant of the temperature-sensitive and attenuation phenotypes. | respiratory syncytial virus (rsv) cpts248/404 is a live-attenuated, temperature-sensitive (ts) vaccine candidate derived from cole-passaged cprsv by two rounds of chemical mutagenesis and biological selection. previous sequence analysis showed that these two steps introduced three single nucleotide substitutions into the cprsv parent. two of these occurred with the coding sequence for the l protein, and each resulted in a single amino acid substitution: gin-831-leu (248 mutation) and asp-1183-gl ... | 1998 | 9705916 |
| protection against respiratory syncytial virus infection by dna immunization. | respiratory syncytial virus (rsv) remains a major cause of morbidity and mortality in infants and the elderly and is a continuing challenge for vaccine development. a murine t helper cell (th) type 2 response associates with enhanced lung pathology, which has been observed in past infant trials using formalin-inactivated rsv vaccine. in this study, we have engineered an optimized plasmid dna vector expressing the rsv fusion (f) protein (dna-f). dna-f was as effective as live rsv in mice at induc ... | 1998 | 9705950 |
| rd6-2198, a novel betain-type fluoroalkylated oligomer, inhibits the replications of human immunodeficiency virus type 1 and other enveloped viruses. | we have examined a novel betain-type fluoroalkylated oligomer, rd6-2198, for its inhibitory effects on the replication of human immunodeficiency virus type 1 (hiv-1) and other enveloped viruses, including herpes simplex virus types 1 and 2 (hsv-1 and hsv-2, respectively) and respiratory syncytial virus (rsv) in cell cultures. we have found that the compound is a potent and selective inhibitor of these viruses. rd6-2198 inhibited the replication of hiv-1iiib at a concentration of 0.85 microg/ml w ... | 1998 | 9707376 |
| aerosolized albuterol improves airway reactivity in infants with acute respiratory failure from respiratory syncytial virus. | the objective of this investigation was to measure the bronchodilator effect of aerosolized albuterol on infants with respiratory syncytial virus (rsv)-induced respiratory failure. infants who required intubation and mechanical ventilator support for rsv disease were eligible for this prospective, nonrandomized study. pulmonary function tests, including respiratory mechanics by least mean square analysis, small airway function by rapid thoraco-abdominal compression, and functional residual capac ... | 1998 | 9710275 |
| immune responses of infants to infection with respiratory viruses and live attenuated respiratory virus candidate vaccines. | respiratory viruses such as respiratory syncytial virus (rsv), the parainfluenza viruses (piv), and the influenza viruses cause severe lower respiratory tract diseases in infants and children throughout the world. experimental live attenuated vaccines for each of these viruses are being developed for intranasal administration in the first weeks or months of life. a variety of promising rsv, piv-3, and influenza virus vaccine strains have been developed by classical biological methods, evaluated ... | 1998 | 9711783 |
| maternal immunization against viral disease. | the protective effect of maternal antibody against many viral diseases has been recognized. the use of maternal immunization has been considered as a means to augment this protection in the young infant against disease. advantages of maternal immunization include the fact that young infants are most susceptible to infections but least responsive to vaccines, that pregnant women are accessible to medical care and respond well to vaccines, that igg antibodies cross the placenta well during the thi ... | 1998 | 9711788 |
| the complete nucleotide sequence of the rice grassy stunt virus genome and genomic comparisons with viruses of the genus tenuivirus. | rice grassy stunt virus (rgsv, irri isolate) has six genomic rna segments. the nucleotide (nt) sequences of rnas 1-4 were determined. the cumulative length of the rgsv genome, including rnas 5 and 6, was 25142 nt. all six rna segments had an ambisense coding strategy and almost identical terminal sequences over 17 nt. the virus complementary (vc) sequence of the largest segment, rna1, had an open reading frame encoding a protein of mr 339133 (the 339.1k protein), while the virus sense (v) sequen ... | 1998 | 9714257 |
| the immunostimulating complex (iscom) is an efficient mucosal delivery system for respiratory syncytial virus (rsv) envelope antigens inducing high local and systemic antibody responses. | iscom is an efficient mucosal delivery system for rsv envelope proteins as measured by antibody responses in respiratory tract secretions and in sera of mice following two intranasal (i.n.) administrations. intranasally administered rsv iscoms induced high levels of iga antibodies both in the upper respiratory tract and in the lungs. in the lungs, a prominent and long-lasting iga response was recorded, which still persisted 22 weeks after the second i.n. immunization when the experiment ended. s ... | 1998 | 9717973 |
| antigenic and genomic diversity of central european respiratory syncytial virus strains. | thirty-two rsv strains recovered during the winter months of 1987/88 to 1993/94 from hospitalized children in vienna, austria and zagreb, croatia were analysed for antigenic and genetic variations. twenty-nine of the 32 isolates investigated belonged to group a and 3 to group b, with the majority of infections caused by subgroup a1 (21 of 29). restriction endonuclease mapping of pcr products derived from parts of the n and g gene of 18 group a strains identified 3 distinct lineages, very similar ... | 1998 | 9722887 |
| palivizumab, a humanized respiratory syncytial virus monoclonal antibody, reduces hospitalization from respiratory syncytial virus infection in high-risk infants. | objective. to determine the safety and efficacy of prophylaxis with palivizumab in reducing the incidence of hospitalization because of respiratory syncytial virus (rsv) infection in high-risk infants. methods. a randomized, double-blind, placebo-controlled trial was conducted at 139 centers in the united states, the united kingdom, and canada. during the 1996 to 1997 rsv season, 1502 children with prematurity (</=35 weeks) or bronchopulmonary dysplasia (bpd) were randomized to receive 5 injecti ... | 1998 | 9724660 |
| subcellular site of expression and route of vaccination influence pulmonary eosinophilia following respiratory syncytial virus challenge in balb/c mice sensitized to the attachment g protein. | the attachment glycoprotein (g) of respiratory syncytial virus (rsv) is synthesized as two mature forms: a membrane-anchored form and a smaller secreted form. mutant cdnas were constructed that encoded one or the other form of the protein and were expressed in recombinant vaccinia viruses (rvv). mice were immunized with rvv by dermal scarification or i.p. injection to determine the contribution of the membrane-anchored and secreted forms of the g protein on the augmentation of pulmonary patholog ... | 1998 | 9725246 |
| 1998 arrs president's award. the potential of in vivo vascular tissue engineering for the treatment of vascular thrombosis: a preliminary report. american roentgen ray society. | current gene therapy and tissue engineering protocols suffer from a number of inherent limitations. in this study, we examine the feasibility of a new approach for the treatment of vascular thrombosis: in vivo tissue engineering. | 1998 | 9725272 |
| characterization of the interaction of the human respiratory syncytial virus phosphoprotein and nucleocapsid protein using the two-hybrid system. | the interaction between the human respiratory syncytial virus phosphoprotein (p) and nucleocapsid (n) protein has been investigated using the two hybrid system in yeast and in tissue culture cells. deletion analysis identified two regions in the p protein involved in this interaction. the immediate carboxy-terminal 20 amino acids were essential for interaction with the n protein. point mutations in this region demonstrated that alteration of two conserved, phosphorylated, serine residues reduced ... | 1998 | 9725669 |
| oncoviral dnas induce transposition of endogenous mobile elements in the genome of drosophila melanogaster. | previously, we have shown that particles of rous sarcoma virus or cloned fragments of rsv cdna as well as dna of oncogenic simian adenovirus sa7, injected into the polar plasm of early drosophila melanogaster embryos, were able to induce, with high frequency, unstable visible mutations in different groups of genetic loci. the genetic instability of the recovered mutations, i.e., their ability to revert to normal state or to generate new mutant alleles at the affected locus, was manifest in mutan ... | 1998 | 9726013 |
| novel anti-respiratory syncytial(rs) viral compounds: benzodithiin derivatives. | benzodithiin derivatives are highly potent and specific inhibitors of respiratory syncytial virus (rsv) replication in vitro. the most potent and selective congener of a benzodithiin derivative is 1,4-dihydro-2,3-benzodithiin(rd3-0028). according to the modified 3-(4,5-dimethylthiazole-2-yl) 2,5-diphenyl tetrazolium bromide (mtt) assay developed in our laboratories, this compound has a 50% effective concentration of 4.5 microm and a 50% cytotoxic concentration of 271.0 microm, which is superior ... | 1998 | 9731237 |
| increased number of t cells committed to il-5 production after respiratory syncytial virus (rsv) infection of human mononuclear cells in vitro. | we examined changes in the cytokine profile of t cells induced by in vitro infection with rsv. isolated mononuclear cells from 27 healthy adults and six infants were infected with rsv at a concentration of 3 moi (multiplicity of infection). after 48 h cells were restimulated with phorbol ester and ionomycin in the presence of monensin for 5 h. the intracellular expression of viral antigen, the cytokines il-2, il-4, il-5, interferon-gamma (ifn-gamma), and the expression of surface markers were as ... | 1998 | 9737676 |
| multiplex pcr for typing and subtyping influenza and respiratory syncytial viruses. | a multiplex reverse transcription (rt)-pcr method that has been developed is capable of detecting and subtyping influenza a (h1n1 and h3n2) and b viruses as well as respiratory syncytial virus (rsv) types a and b in respiratory clinical samples taken as part of a national community-based surveillance program of influenza-like illness in england and wales. the detection of each different pathogen depended on distinguishing five amplification products of different sizes on agarose gels following r ... | 1998 | 9738055 |
| palivizumab, a humanized respiratory syncytial virus monoclonal antibody, reduces hospitalization from respiratory syncytial virus infection in high-risk infants. the impact-rsv study group. | to determine the safety and efficacy of prophylaxis with palivizumab in reducing the incidence of hospitalization because of respiratory syncytial virus (rsv) infection in high-risk infants. | 1998 | 9738173 |
| polymerase chain reaction-based detection of rhinovirus, respiratory syncytial virus, and coronavirus in otitis media with effusion. | to study the association of human rhinovirus (hrv), respiratory syncytial virus (rsv), and human coronavirus infections in children aged 6 months to 12 years with otitis media with effusion (ome). to determine how long hrv rna can be detected after hrv infection. | 1998 | 9738723 |
| neuroprotection of spinal motoneurons following targeted transduction with an adenoviral vector carrying the gene for glial cell line-derived neurotrophic factor. | application of neurotrophic factors (nfs) to the cut stump of peripheral nerves confers transient (1- to 2-week) neuroprotection of motoneurons from axotomy-induced death in neonates. we tested whether lumbar spinal motoneurons would be protected from axotomy-induced death when they were genetically modified to produce nfs in situ. adenoviral (adv) vectors carrying neurotrophic factor genes under control of the rous sarcoma virus long terminal repeat promoter (adv.rsv-nf) or a control vector con ... | 1998 | 9743571 |
| seasonal trends of viral respiratory tract infections in the tropics. | to evaluate the seasonal trends of viral respiratory tract infections in a tropical environment, a retrospective survey of laboratory virus isolation, serology and immunofluorescence microscopy in two large general hospitals in singapore between september 1990 and september 1994 was carried out. respiratory tract viral outbreaks, particularly among infants who required hospitalization, were found to be associated mainly with respiratory syncytial (rsv) infections (72%), influenza (11%) and parai ... | 1998 | 9747763 |
| the effect of fluticasone propionate on respiratory syncytial virus-induced chemokine release by a human bronchial epithelial cell line. | respiratory syncytial virus (rsv) is an important cause of bronchiolitis in infants, is an important trigger of asthma exacerbation, and stimulates chemokine production by human respiratory epithelial cells in vitro. we tested the effect of the corticosteroid fluticasone propionate (fp) on rsv-stimulated production of the chemokines interleukin 8 (il-8) and rantes (regulated upon activation, normal t cell expressed and secreted) by a human bronchial epithelial cell line, beas-2b. confluent beas- ... | 1998 | 9754905 |
| an avian cdna encoding a tyrosine-phosphorylated protein with pdz, coiled-coil, and sam domains. | tyrosine phosphoproteins of size 115-120 kda were purified from membranes of chicken embryo fibroblasts (cef) infected with rous sarcoma virus (rsv). a mouse was immunized with these proteins, and the immune serum was used to screen a cef cdna expression library. a highly immunoreactive clone (ks5) was identified and characterized. the cdna of this clone is 2.3 kb in length with a short 5' utr and a single major open reading frame (orf) encoding a polypeptide of 719 amino acids, with a calculate ... | 1998 | 9757012 |
| aggregation independent of n-cadherin and neural cell adhesion molecule on quail myoblasts transformed with temperature-sensitive rous sarcoma virus. | quail myoblasts transformed with the temperature-sensitive mutant of rous sarcoma virus (qm-rsv cells) differentiate temperature-sensitively. at 41 degrees c, the cells begin to fuse after about 15-18 h and form multinucleated myotubes, whereas, at 35.5 degrees c, the cells proliferate. tyrosine-phosphorylation relates to this temperature-sensitive differentiation. in the course of the investigation of qm-rsv cells, when qm-rsv cells were dissociated with edta and shaken in dmem, the aggregation ... | 1998 | 9759520 |
| neutrophils induce damage to respiratory epithelial cells infected with respiratory syncytial virus. | the mechanisms by which respiratory syncytial virus (rsv) infection induces bronchiolitis and airway disease are unclear. the presence of large numbers of polymorphonuclear leukocytes (pmn) in the airways of infants with rsv infection suggests a potential role of pmn in airway injury associated with rsv infection. to investigate the potential role of neutrophils in rsv bronchiolitis, human alveolar type ii cells (a549 cells) were infected with different doses of rsv for 6-48 h. a 51cr-releasing ... | 1998 | 9762789 |
| the apoptosis of neutrophils is accelerated in respiratory syncytial virus (rsv)-induced bronchiolitis. | neutrophils are the predominant inflammatory cell in the lung tissues and airways in rsv infection, and can augment the epithelial cell damage induced by rsv. neutrophil apoptosis has been suggested to be a mechanism to reduce the potential for tissue injury. the apoptosis of neutrophils from nasopharyngeal aspirates (npa) (n = 19) and peripheral blood (pb) of infants with rsv bronchiolitis (n = 11) and pb from healthy controls (n = 9) was investigated. monoclonal antibody against cd95 (fas) and ... | 1998 | 9764602 |
| cytotoxic t-lymphocyte precursor frequencies in balb/c mice after acute respiratory syncytial virus (rsv) infection or immunization with a formalin-inactivated rsv vaccine. | a better understanding of the immune response to live and formalin-inactivated respiratory syncytial virus (rsv) is important for developing nonlive vaccines. in this study, major histocompatibility complex (mhc) class i- and ii-restricted, rsv-specific cytotoxic t-lymphocyte precursor (ctlp) frequencies were determined in bronchoalveolar lavage (bal) samples and spleen lymphocytes of balb/c mice intranasally infected with live rsv or intramuscularly inoculated with formalin-inactivated rsv (fi- ... | 1998 | 9765442 |
| importance of basic residues in the nucleocapsid sequence for retrovirus gag assembly and complementation rescue. | the gag proteins of rous sarcoma virus (rsv) and human immunodeficiency virus (hiv) contain small interaction (i) domains within their nucleocapsid (nc) sequences. these overlap the zinc finger motifs and function to provide the proper density to viral particles. there are two zinc fingers and at least two i domains within these gag proteins. to more thoroughly characterize the important sequence features and properties of i domains, we analyzed gag proteins that contain one or no zinc finger mo ... | 1998 | 9765448 |
| the effects of virus-specific antibodies on the replication of bovine respiratory syncytial virus in vitro and on clinical disease and immune responses in lambs. | low concentrations of antibodies, specific to human respiratory syncytial virus (rsv) have been shown to enhance virus replication in human monocytic cell lines by several workers. in the present study, replication of bovine rsv in ovine peripheral blood monocytes was shown to be enhanced in the presence of low concentration of bovine rsv-specific antibodies. antibodies had no enhancing effect on virus replication in secondary lamb testis cells or monocytic cell lines derived from peripheral blo ... | 1998 | 9643456 |
| acute lower respiratory infections due to respiratory syncytial virus and adenovirus among hospitalized children from argentina. | acute lower respiratory infection (alri) is one of the main causes of morbidity and mortality in small children. | 1998 | 9645999 |
| eosinophil cationic protein/rnase 3 is another rnase a-family ribonuclease with direct antiviral activity. | eosinophil cationic protein (ecp) is one of two rnase a-superfamily ribonucleases found in secretory granules of human eosinophilic leukocytes. although the physiologic function of eosinophils [and thus of the two eosinophil ribonucleases, ecp and eosinophil-derived neurotoxin (edn)] remains controversial, we have recently shown that isolated human eosinophils promote ribonuclease-dependent toxicity toward extracellular virions of the single-stranded rna virus, respiratory syncytial virus, group ... | 1998 | 9649619 |
| human t-cell leukemia virus type 1 reverse transcriptase (rt) originates from the pro and pol open reading frames and requires the presence of rt-rnase h (rh) and rt-rh-integrase proteins for its activity. | the first description of an active form of a recombinant human t-cell leukemia virus type 1 (htlv-1) reverse transcriptase (rt) and subsequent predictions of its amino acid sequence and quaternary structure are reported here. by using amino acid alignment methods, the nh2 and cooh termini of the rt, rnase h (rh), and integrase (in) domains of the pol polyprotein were determined. the htlv-1 rt seems to be unique since its nh2 terminus is probably encoded by the pro open reading frame (orf) fused ... | 1998 | 9658093 |
| role of the transcription start site core region and transcription factor yy1 in rous sarcoma virus long terminal repeat promoter activity. | the rous sarcoma virus (rsv) long terminal repeat (ltr) contains a transcriptionally potent enhancer and promoter that functions in a variety of cell types. previous studies have identified the viral sequences required for enhancer activity, and characterization of these elements has provided insight into the mechanism of rsv transcriptional activity. the objective of this study was to better define the rsv ltr promoter by examining the transcription start site core (tssc) region. deletion of th ... | 1998 | 9658104 |
| adenovirus-mediated gene transfer in vivo to cerebral blood vessels and perivascular tissue in mice. | gene transfer to cerebral blood vessels has been accomplished in rats and dogs by injection of replication-deficient adenovirus into cerebrospinal fluid. in this study we examined transgene expression after injection of adenovirus into the cerebrospinal fluid of mice. responses were observed in icr mice and c57bl/6 mice, which are outbred and inbred strains, respectively. | 1998 | 9660397 |
| respiratory syncytial virus-induced interleukin-4 production by human conjunctival epithelial cells contributes to allergy: preliminary study. | we previously reported allergic conjunctivitis was associated with a local pattern of cytokines and identified respiratory syncytial virus (rsv) in the conjunctival epithelium, in patients with allergic conjunctivitis. we hypothesized that rsv infected the normal conjunctival epithelium and produced interleukin-4 (il-4), causing an allergic reaction. to determine this event, we studied production of il-4 by human conjunctival epithelial cells, after infection with rsv. | 1998 | 9663856 |
| respiratory syncytial virus vaccines. | respiratory syncytial virus (rsv) is the most important cause of viral lower respiratory tract illness (lri) in infants and children worldwide and causes significant lri in the elderly and in immunocompromised patients. the goal of rsv vaccination is to prevent serious rsv-associated lri. there are several obstacles to the development of successful rsv vaccines, including the need to immunize very young infants, who may respond inadequately to vaccination; the existence of two antigenically dist ... | 1998 | 9665976 |
| characterization of myogenin expression in myotubes derived from quail myoblasts transformed with a temperature sensitive mutant of rous sarcoma virus. | during myogenic differentiation of quail myoblasts transformed with a temperature sensitive mutant of rous sarcoma virus (qm-rsv cells), it was observed that myogenin was continuously expressed in myotubes. in contrast, in myotubes derived from quail primary cells (parent cells of qm-rsv cells), myogenin expression was seen only in the myotubes not having striated structures comprised of myofibrils, but not in myotubes having the structures. the fact that there are not striated structures of myo ... | 1998 | 9669033 |
| development of transgenic xenopus laevis with a high c-src gene expression. | xenopus laevis larvae with an elevated expression of c-src were generated by mating a transgenic x. laevis male frog carrying proviral rous sarcoma virus (rsv) long terminal repeat (ltr) and most of the pol gene sequences in its sperm dna and a normal x. laevis female frog. offspring (15-20%) with a higher dosage of c-src, detected in disorganized myotomal musculature and in cerebral and spinal neuronal cells by immunohistochemical analysis, developed abnormally, with edemas (in most cases), hea ... | 1998 | 9669525 |
| t cell subset analysis in peripheral blood of children with rsv bronchiolitis. | respiratory syncytial virus (rsv) bronchiolitis is partially an immune-mediated disease in which rsv-specific t cells play a predominant role. the peripheral blood t cell response was studied in patients with rsv bronchiolitis in order to detect evidence for t cell redistribution during natural rsv infection and if so, which subsets are involved. 18 patients with rsv bronchiolitis and 13 control patients were studied. the white blood cell count, the number of t cells and t cell subsets in blood ... | 1998 | 9670363 |
| respiratory syncytial virus-induced rantes production from human bronchial epithelial cells is dependent on nuclear factor-kappa b nuclear binding and is inhibited by adenovirus-mediated expression of inhibitor of kappa b alpha. | respiratory syncytial virus (rsv) infection is an important cause of lower respiratory tract illness, the severity of which may be partly due to cellular recruitment. rsv infection activates chemokine secretion from airway epithelial cells by largely unknown mechanisms. we investigated the regulation of rsv-induced activation of the chemokine rantes in the bronchial epithelial cell line beas-2b and primary normal human tracheobronchial epithelial cultures. rantes protein and mrna were detected a ... | 1998 | 9670982 |