Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [synthesis and antiparasitic activity of new n-alkyl derivatives of 1-(nitrophenyl)-3-(methyl-3-indolyl)-prop-2-ene-1-ones (nitroindolylchalcones)]. | 1996 | 9008902 | |
| [impact of new methodologies in the study of parasites]. | 1996 | 9011513 | |
| differentiation of entamoeba histolytica and entamoeba dispar in cyst-passers by immunoblot. | differentiation of invasive strains of entamoeba histolytica according to their pathogenicity has been a topic of long debate, but now the pathogenic species only is regarded as e. histolytica while the non pathogenic species is e. dispar. the present study applied immunoblot to differentiate infections of the two species among microscopically-detected cyst-passers in korea. the crude extract of e. histolytica separated in 5.20% gradient gels, revealed many fractions of 94, 81, 71, 50, 44, 38.5, ... | 1996 | 9017910 |
| does anti-parasitic treatment normalize platelets morphology in patients infested with entamoeba histolytica? | platelets are part of body defence system, especially the anti-parasite immunologic response. platelets manifest their functions only after their activation. thrombine activates platelets inducing change of their shape and causing secretion of certain substances. this study was designed to estimate platelet morphology as an indicator of their activation and effectiveness of anti-parasite therapy. the study was conducted in group of 30 patients infested with e. histolytica before treatment (a) an ... | 1996 | 9020537 |
| rapid postmortem invasion of cecal mucosa of macaques by nonpathogenic entamoeba chattoni. | although entamoeba histolytica is the third leading parasitic cause of death in the world, most infections in humans are asymptomatic and restricted to the intestinal lumen. entamoeba histolytica infections have also been reported in most species of captive nonhuman primates, with new world monkeys being particularly susceptible to fatal invasive amebiasis. in contrast, old world monkeys appear to be resistant to the disease, although tissue invasion in asymptomatic monkeys has been reported. ou ... | 1996 | 9025684 |
| seroepidemiology of entamoeba histolytica in a slum in northeastern brazil. | infection with the human pathogenic parasite entamoeba histolytica has not been well-characterized in northeastern brazil. in this study, the prevalence of e. histolytica infection in a slum in northeastern brazil was assayed using an enzyme-linked immunosorbent assay (elisa) for antibodies against the galactose/n-acetyl-d-galactosamine (gal/galnac)-inhibitable adherence lectin of e. histolytica. sera from a total of 335 individuals were examined for anti-gal/galnac lectin antibodies. the overal ... | 1996 | 9025700 |
| stable episomal transfection and gene expression in entamoeba dispar. | 1996 | 9027760 | |
| pyruvate: ferredoxin oxidoreductase from entamoeba histolytica recognized by a monoclonal antibody. | a mouse monoclonal antibody, eh208c2-2 mab, raised against whole cell antigens of entamoeba histolytica trophozoites of the pathogenic strain hm-1: imss and polyclonal antisera (pab) against membrane antigens of e. histolytica trophozoites of strain hth-56: mutm were screened against a cdna library of the pathogenic strain, sfl3. the monoconal antibody detected many phage plaques expressing an e. histolytica protein. the dna sequence encoding the protein was approximately 55% identical, over 1,1 ... | 1996 | 9031403 |
| prevalence of intestinal parasites in adult patients with enteropathic aids in north-eastern tanzania. | a six month study was conducted in north-eastern tanzania to determine the prevalence of pathogenic intestinal parasites among adult patients with enteropathic aids. a total of 352 patients were recruited of whom 158 (45%) had chronic diarrhoea. of the 352 patients, 123 (35%) had intestinal parasites. of the 123, 77 (62.6%) patients had chronic diarrhoea. the types of parasites detected were cryptosporidium, isospora belli, strongyloides stercoralis, schistosoma mansoni, trichuris, trichiura, as ... | 1996 | 8840602 |
| incidence of protozoal diarrheal disease in an expatriate community in addis ababa. | the incidence of diarrheal disease due to entamoeba histolytica and giardia lamblia in the united states embassy american population was recorded over a 25 month period. overall case rates of 4.2/100 person-months for amoebiasis and 3.3/100 person-months for giardiasis were distinguished by wide variations based on age group and rainfall. in some circumstances, gender and prior living in africa also correlated with disease incidence. practitioners should look for these entities in american expat ... | 1996 | 8840612 |
| amoebiasis of the female genital tract: a case report. | in 1994, a 56-year-old burmese woman presented with bloody discharge per vaginam had undergone pelvic examination disclosing multiple small ulcerated and friable lesions with contact bleeding at vulva, vagina and cervix. wet smears from vaginal discharge showed typical entamoeba histolytica trophozoites. the patient was treated with metronidazole 750 mg three times daily for 7 days, resulting in complete recovery of the lesions in 10 days. the pathology, risk factors, diagnosis and management of ... | 1996 | 8840708 |
| a single-step immunochromatographic test for the detection of entamoeba histolytica antigen in stool samples. | a rapid, single step, sensitive and specific immunochromatographic test for the detection of e. histolytica antigens in the stool samples is described. monoclonal antibody ac55 specific for e. histolytica was used as capture antibody on nitrocellulose sheet. the same antibody was conjugated to colloidal gold to detect the presence of antigen. in this test, multiple steps of washing, incubation, etc. are not required and there is no need for secondary colour development as gold particles produce ... | 1996 | 8841457 |
| protection against amebic liver abscess formation in the severe combined immunodeficient mouse by human anti-amebic antibodies. | we have used serum from patients with amebic liver abscess to investigate the role of antibody in the prevention of invasive amebiasis using the severe combined immunodeficient (scid) mouse model of entamoeba histolytica infection. the scid mice were passively immunized with serum or purified antibody from patients with amebic liver abscess 24 hr prior to the direct intrahepatic challenge with 10(6) virulent e. histolytica trophozoites. this treatment reduced the mean abscess size in these anima ... | 1996 | 8842124 |
| cytology of gastrointestinal histoplasmosis. a report of two cases with differential diagnosis and diagnostic pitfalls. | gastrointestinal (gi) histoplasmosis is a rare manifestation of histoplasma capsulatum (hc) infection. there are no reports of its cytologic diagnosis in the literature. | 1996 | 8842180 |
| comparative evaluation of somatic & excretory-secretory antigens of entamoeba histolytica in serodiagnosis of human amoebiasis by elisa. | the excretory-secretory antigens collected from the axenic culture medium and conventional somatic antigen prepared from the whole trophozoites of e. histolytica were compared for their efficacy in serodiagnosis of amoebiasis. a total of 280 sera collected from different clinically proven cases of amoebiasis and healthy subjects were analysed against both the antigens in elisa. both antigens showed a 100 per cent correlation in amoebic liver abscess cases, patients infected with enteropathogens ... | 1996 | 8783520 |
| partial purification and characterization of ornithine decarboxylase from entamoeba histolytica. | multiplication of e. histolytica was accompanied by a parallel increase in ornithine decarboxylase (odc) specific activity up to 72 h of cultivation in tyi-s-33 medium. thereafter, activity rapidly decayed whereas growth continued for another 24 h before entering into the stationary growth phase. odc was very unstable. partial purification (14-fold) of the enzyme was achieved by a three-step procedure involving high-speed centrifugation, gel filtration and adsorption to hydroxylapatite. the part ... | 1996 | 8786214 |
| molecular evolution of the 14-3-3 protein family. | members of the highly conserved and ubiquitous 14-3-3 protein family modulate a wide variety of cellular processes. to determine the evolutionary relationships among specific 14-3-3 proteins in different plant, animal, and fungal species and to initiate a predictive analysis of isoform-specific differences in light of the latest functional and structural studies of 14-3-3, multiple alignments were constructed from forty-six 14-3-3 sequences retrieved from the genbank and swissprot databases and ... | 1996 | 8798343 |
| sequence-specific detection of pcr-amplified dna by restriction enzyme release of hybrids. | we have developed a method to detect pcr amplicons that contain a restriction enzyme site. the method is called polymerase chain reaction-hybridization and enzymatic release assay (pcr-hera). the pcr product is hybridized to a synthetic oligonucleotide that is immobilized on a microtitre plate. specific cleavage of the resulting hybrid by a restriction endonuclease liberates a fluorescein labeled arm of the immobilized dna. we demonstrate the use of pcr-hera to detect species-specific sequences ... | 1996 | 8799371 |
| entamoeba histolytica: proteinase secretion induced by collagen type i is dependent on cytoskeleton integrity. | proteolytic activities of the protozoan parasite entamoeba histolytica strain hm1:imss and the cytochalasin d-resistant mutant bg-3 were analyzed following stimulation with collagen type i and ca2+, which induces electron-dense associated collagenase secretion. the mutant bg-3 had a protease activity of 73 kda and secretion of total protease activity was not stimulated by collagen type i and ca2+, which produced, in contrast, a 2-fold increase in protease secretion by the parental strain. this c ... | 1996 | 8801549 |
| intestinal parasites among the southeast asian laborers in taiwan during 1993-1994. | a large number of foreign laborers are now working in taiwan, the majority coming from southeast asia where parasitic infections are common. since 1992, monitoring the intestinal parasitic infections of these foreigners has been carried out, particularly to prevent the introduction of some of the parasites into taiwan. this paper reports results obtained during the year 1993-1994. | 1996 | 8803301 |
| relative importance of inositol (1,4,5)trisphosphate and inositol (1,3,4,5)tetrakisphosphate in entamoeba histolytica. | [3h]inositol tetrakisphosphate (ins(1,3,4,5)p4) binding sites which were poorly displaced by unlabelled inositol (1,4,5)-trisphosphate (ins(1,4,5)p3) were detected in membrane fractions of entamoeba histolytica. similarly, unlabelled ins(1,3,4,5)p4 was 30-fold less efficient in displacing [3h]ins(1,4,5)p3 binding. ph sensitivities of binding of the two isomers were markedly different. scatchard analysis of the data revealed single binding sites and similar receptor densities for each of the two ... | 1996 | 8804436 |
| analysis of 69 patients with amebic liver abscess. | all 69 patients with amebic liver abscess that we treated in 1981-1992 were studied retrospectively. men predominated by a 10:1 ratio. of our 227 patients with amebiasis, some 30% yearly had liver involvement. the incidence peaked in 1988, decreasing later but increasing again in 1992. most patients were 30-50 years old, the overall mean age being 45 years (range, 22-79), and decreasing with time. patients with the related factors of travel abroad, positive results of a test for treponema pallid ... | 1996 | 8808427 |
| sequence of a giardia lamblia gene coding for the glycolytic enzyme, pyruvate,phosphate dikinase. | the sequence of the gene coding for pyruvate,phosphate dikinase (ec 2.7.9.1) in giardia lamblia (syn. g. duodenalis) has been established. the deduced amino acid sequence is very similar to all of its known homologs from the protist, entamoeba histolytica, the eubacterium, clostridium symbiosum and plant chloroplasts. phylogenetic reconstruction with neighbor-joining and maximum parsimony methods reveals that the sequences form two clades, one comprising the anaerobic microorganisms, and the oth ... | 1996 | 8813667 |
| a homologue of the cysteine proteinase gene (acp1 or eh-cpp3) of pathogenic entamoeba histolytica is present in non-pathogenic e. dispar strains. | one of the three cysteine proteinase genes, acp1 (or cp 3), has been reported to be missing in non-pathogenic strains of entamoeba histolytica (or entamoeba dispar as recently labeled). unexpectedly, a gene fragment very similar in its sequence (95% homology) to acp1 of pathogenic strains was obtained by use of the polymerase chain reaction from genomic dna and cdna of various cloned non-pathogenic strains as well as in 23 clinical isolates from asymptomatic carriers. the finding of the acp1 hom ... | 1996 | 8813676 |
| upstream regulatory elements controlling expression of the entamoeba histolytica lectin. | entamoeba histolytica genomic organization and putative promoter elements appear to be distinct from both metazoan and better characterized protozoan organisms. the recent development of dna-mediated transfection for e. histolytica enabled characterization of cis-acting promoter elements required for gene expression. a deletion and replacement analysis was conducted on the promoter of an e. histolytica gene encoding the heavy subunit of the n-acetyl-beta-d-galactosamine-specific adhesin (hgl5). ... | 1996 | 8813680 |
| cloning and characterization of the entamoeba histolytica pyruvate: ferredoxin oxidoreductase gene. | 1996 | 8813698 | |
| recombinant antigen-based avidin-biotin microtiter enzyme-linked immunosorbent assay for serodiagnosis of invasive amebiasis. | an immunoscreening approach was used to isolate a strongly positive cdna clone from an entamoeba histolytica hk-9 cdna expression library in the phage vector lambda zap-ii. the 1.85-kb cdna insert was found to be truncated and encoded the cysteine-rich, immunodominant domain of the antigenic 170-kda subunit of the amebal galactose-n-acetylgalactosamine binding lectin. this domain was expressed as a glutathione s-transferase fusion protein in escherichia coli. inclusion bodies of the recombinant ... | 1996 | 8815092 |
| differentiation of korean isolates of entamoeba histolytica from entamoeba dispar. | cysts of entamoeba histolytica are still found from humans in korea, but not all of the cysts are known as pathogenic. the non-pathogenic strain is regarded as a different species, e. dispar. in this study, korean isolates of conventional e. histolytica were subjected for the differentiation by polymerase chain reaction (pcr) and restriction fragment length polymorphism (rflp) analysis. human stools were screened by routine microscopic examination, and cyst or trophozoite positive stools were in ... | 1996 | 8820737 |
| physical mapping and expression of gene families encoding the n-acetyl d-galactosamine adherence lectin of entamoeba histolytica. | adherence of the enteric protozoan parasite entamoeba histolytica is mediated by an n-acetyl d-galactosamine (galnac)-specific lectin, a heterodimer of heavy (170 kda) and light (35/31 kda) subunits. the gene families encoding the lectin subunits were characterized using clamped homogeneous electric field (chef) gel electrophoresis in the strain hm1:imss. the heavy subunit was shown to be encoded by a family of five hgl genes, which were physically mapped to five distinct hindiii restriction fra ... | 1996 | 8821939 |
| [invasive amebiasis at an institution for the mentally retarded in shizuoka prefecture]. | amebiasis caused by entamoeba histolytica at an institution for mentally retarded in shizuoka prefecture is reported. five of the 50 patients showed e. histolytica cysts in their stools and 4 were positive serologically. the polymerase chain reaction and restriction fragment length polymorphism revealed that the isolates were pathogenic-type e. histolytica. epidemiological analysis revealed that the amebic infection was caused by the abnormal behavior of mentally retarded patients. administratio ... | 1996 | 8822047 |
| a gene highly homologous to acp1 encoding cysteine proteinase 3 in entamoeba histolytica is present and expressed in e. dispar. | we have cloned the complete gene encoding cysteine proteinase 3 in e. histolytica as well as a cdna encoding a highly homologous protein in e. dispar. in addition, we have demonstrated the presence and expression of the cp3 gene in various e. histolytica and e. dispar isolates. since the expression of the gene is rather similar within both entamoeba species, we assume that it does not constitute the proposed virulence factor of e. histolytica. | 1996 | 8825217 |
| detection and characterization of dna polymerase activity in entamoeba histolytica. | dna polymerase activity was detected and characterized in nuclear extracts from trophozoites of entamoeba histolytica. the activity was high at ph 2 to ph 6, but at ph 8 and 10 the activity was very low. the presence of k+ was inhibitory for the activity and a higher concentration of k+ markedly inhibited the activity. magnesium ions (mg2+) were absolutely required for activity and its optimal concentration was 6 to 8 mm. the activity was markedly inhibited by aphidicolin which is an inhibitor o ... | 1996 | 8825452 |
| recent advances in amebiasis. | advancements in our understanding of amebiasis have been rapid over the decade that i have followed this field. what was identified morphologically for years as entamoeba histolytica has been redescribed with modern techniques as a complex of two species, the commensal parasite e. dispar and the pathogenic parasite e. histolytica that is the cause of colitis and liver abscess. antigen detection tests are now available for the rapid detection in stool of the pathogenic species e. histolytica. new ... | 1996 | 8833626 |
| isoenzyme profile as parameter to differentiate pathogenic strains of entamoeba histolytica in brazil. | the isoenzyme profiles (ip) of 33 strains of entamoeba histolytica isolated from patients and carriers of two regions in brazil (amazonia and southeast) were determined. the enzymes phosphoglucomutase, glucose-phosphate isomerase, hexokinase and malic enzyme were considered. ip of the strains was correlated with culture conditions, time of maintenance in laboratory and clinical history of patients. the strains were maintained under polyxenic, monoxenic and axenic culture conditions: 27 polyxenic ... | 1996 | 9293086 |
| application of local products r-phycoerythrin and monoclonal antibody as a fluorescent antibody probe to detect entamoeba histolytica trophozoites. | r-phycoerythrin (r-pe) was extracted from red algae, gracilaria fisheri from pattani province, thailand, with 50 mm sodium phosphate buffer, ph 7.0, followed by precipitation with 30-50% final concentration of saturated ammonium sulphate solution at 0 degree c. the precipitate was further purified by deae-cellulose (de-52) column chromatography. the purified r-pe showed a single band of mr 240 kda by polyacrylamide gel electrophoresis with the maximum absorption and maximum fluorescence emission ... | 1996 | 9279993 |
| detoxicating enzymes of entamoeba histolytica and their detoxifying roles. | to investigate the entamoeba histolytica living in the low oxygen concentration colon of the host and how does it survive in the circumstance after invading the tissues with high oxygen concentration while obtaining oxygen without being damaged by the toxins. | 1996 | 9275357 |
| monoclonal antibody-based elisa for the detection of circulating entamoeba histolytica antigen in hepatic amebiasis in hamsters (mesocricetus auratus). | circulating amebic antigens were determined by using a sandwich elisa with specific monoclonal antibody in the sera of 35 group i hamsters, 7 of which were sacrificed at intervals after hepatic inoculation with 500,000 axenically grown hm:1:imss strain of e. histolytica trophozoites, 7 group ii infected hamsters in which metronidazole treatment was given and 18 group iii uninfected controls. amebic antigenemia was demonstrated in 5 of 7 (71.4%), 6 of 7 (85.7%), 7 of 7 (100%), 7 of 7 (100%) and 7 ... | 1996 | 9253880 |
| [seasonality of parasites and intestinal bacteria in vegetables that are consumed raw in costa rica]. | in costa rica, a total of 640 samples from eight different vegetables used for raw consumption, were analyzed for the presence of intestinal parasites and fecal coliforms. eighty samples of each vegetable were analyzed, forty during the dry season and forty in the rainy. a greater, but unsignificant (p > 0.05) level of fecal coliforms was found during the dry season. levels of escherichia coli, were higher (p < 0.05) during the dry season in lettuce (latuca sativa) and cilantro (coleandrum sativ ... | 1996 | 9246361 |
| evidence for the existence of ganglioside molecules in the antigen of entamoeba histolytica. | gangliosides were found to be present in entamoeba histolytica. they were extracted from lyophilized trophozoites of the pathogenic strain hm-1:imss and purified by high performance thin-layer chromatography. two resorcinol-positive bands, comigrating with gm2 and gd1a were demonstrated, revealing the existence of ganglioside molecules in entamoeba histolytica. the gm2 content, determined as lipid-bound sialic acid, was 1.5 micrograms/10(8) amoebae, the content of the gd1a comigrating band was 0 ... | 1996 | 9223167 |
| gene amplification in entamoeba histolytica. | we show here data suggesting that entamoeba histolytica, the protozoan responsible for human amebiasis, presents dna amplification in a fashion similar to that described for transformed mammalian cells. by transmission electron microscopy (tem), we found linear, circular and concentric circular dna molecules exhibiting the main events of the unscheduled dna amplification process. loops were formed after the recombination of two nonadjacent dna regions, and bubbles appeared from the recombinant s ... | 1996 | 9371226 |
| application of polymerase chain reaction for diagnosing amebic liver abscess. | polymerase chain reaction (pcr) has been applied in diagnosing amebic liver infection by detecting pathogenic entamoeba histolytica dna in liver aspirates. oligonucleotide primers found to be specific for the gene encoding the 30 kda molecule of this pathogenic ameba were used in the test. liver aspirates obtained from 23 patients with amebic liver abscess substantiated by typical clinical manifastation or with very high titres of anti-e histolytica antibodies by elisa were found to be positive ... | 1996 | 9387418 |
| prevalence and etiology of intestinal parasites in lebanon. | as there are no studies done on the prevalence of intestinal parasites in lebanon since 1967, this study was undertaken to reveal the current patterns of intestinal parasitic infestations in lebanese patients from two geographic regions: beirut and tripoli. analysis was based on 33,253 stool specimens examined at the american university of beirut medical center (auh) and 11,611 specimens examined at the islamic hospital (ih) in tripoli over five and three years, respectively. the prevalence of i ... | 1996 | 9260400 |
| [presence of various pathogenic microorganisms in fresh vegetables in costa rica]. | this study reports the occurrence of some pathogenic microorganisms in vegetable consumed on a daily basis by costa ricans. cryptosporidium sp. oocysts were found in 5.2% (4/80) of cilantro leaves, in 8.7% (7/80) of cilantro roots and 2.5% of lettuce samples. a 1.2% (1/80) incidence was found in other vegetables samples (carrot, cucumber, radish and tomatoe). oocysts of this parasite were absent in cabbage. giardia intestinalis was only detected in 5.2% (4/80) of cilantro leaves and in 2.5% (2/ ... | 1996 | 9429609 |
| surface localization, regulation, and biologic properties of the 96-kda alcohol/aldehyde dehydrogenase (ehadh2) of pathogenic entamoeba histolytica. | the 96-kda surface antigen of entamoeba histolytica was demonstrated through extensive immunologic evaluation with monoclonal and monospecific antibodies to be identical to or an isoform of the amebic alcohol/aldehyde dehydrogenase (ehadh2). ehadh2 was secreted, excreted, or shed into the culture medium in quantities commensurate with amebic growth when studied in a novel culture system. of importance, using rnase protection assays, specific mrna coding for the ehadh2 gene product(s) was up-regu ... | 1996 | 8537663 |
| entamoeba histolytica encodes a highly divergent beta-tubulin. | the microtubules of the amitochondrial parasite entamoeba histolytica are atypical in certain respects. consistent with this, we report that e. histolytica encodes the most divergent beta-tubulin identified to date, with only 54% to 58% identity to beta-tubulins from various species. a similarly divergent beta-tubulin is encoded by the related entamoeba invadens; single gene copies appear to be present in both organisms. the entamoeba sequences were compared with a database of 101 beta-tubulins, ... | 1996 | 8563707 |
| [enteropathogenic agents isolated in persistent diarrhoea]. | out of 45558 patients admited in our hospital, from january 1990 to december 1993, the clinical records of 4445 children with diarrhoea (4289 with acute diarrihoea and 156 with persistent diarrhoea) were reviewed. those with positive bacteriological or parasitological results were taken as sample. salmonella was the bacteria most frequently isolated in both groups of patients. ecep and eceh had a similar distribution while shigella showed a higher frequency in cases of persistent diarrhoea, for ... | 1996 | 12165786 |
| recent advances in dna-mediated gene transfer of entamoeba histolytica. | during the past few years, the introduction of dna-mediated gene transfer into parasite research has permitted subtle studies on fundamental aspects of parasite biology. in this paper, egbert tannich describes the recent breakthrough of successful entamoeba histolytica transfection, and the subsequent developments in this field. | 1996 | 15275214 |
| modulation of host cell intracellular ca2+. | during the course of evolution, protozoan parasites have developed strategies to subvert the immune response of their host in order to multiply, reproduce and survive. one of these inherited strategies is their capacity to modulate the host cell transductional mechanisms in their favor. alteration of host cells ca(2-) homeostasis following interaction and/or invasion by protozoan parasites such as leishmania donovani, trypanosoma cruzi, plasmodium falciparum or entamoeba histolytica has been rep ... | 1996 | 15275223 |
| differentiation of entamoeba histolytica and entamoeba dispar infections. | in this article, terry jackson and jonathan ravdin briefly review the latest information on monoclonal antibody-based elisas that use antigen capture as a tool in the differential detection of human infection with entamoeba histolytica and e. dispar. current technology of culture and isoenzyme analysis is not widely available, is cumbersome and too time-consuming. a further potential benefit of antigen detection tests is that they can be used to monitor the efficacy of therapy; this is a shortco ... | 1996 | 15275293 |
| progress towards an amebiasis vaccine. | amebiasis (infection by entamoeba histolytica) remains a major health problem in much of the developing world. morbidity and mortality from amebic dysentery and amebic liver abscess have persisted despite the availability of effective anti-amebic therapy, suggesting a need for alternative measures of disease control. through the application of recombinant dna technology, several e. histolytica antigens have now been expressed in prokaryotic systems and tested in animal models as vaccines to prev ... | 1996 | 15275302 |
| the invasiveness of entamoeba histolytica - a continuing enigma. | 1996 | 16696073 | |
| association of parasites and diarrhoea among children less than five years of age in a rural area in egypt. | a case control study was conducted in kafr el-sheikh governorate, egypt, a rural community, to assess the relation of parasites and diarrhoea among children <5 years. a sample of 196 cases with diarrhoea and 83 non-diarrhoeic controls were included in the study. a questionnaire was completed for every child and weight and height were recorded. a stool sample was obtained and examined for parasites present. the results revealed that 70.3% of cases had parasites compared to 59% of controls. the mo ... | 1996 | 17214190 |
| treatment of symptomatic amebic colitis in human immunodeficiency virus-infected persons. | while most entamoeba histolytica appearing in male homosexuals infected with human immunodeficiency virus (hiv) is considered non-invasive in western countries, and treatment of amebiasis in these persons has received very little attention, in japan some male homosexual amebiasis patients infected with hiv complain of symptoms attributable to e. histolytica infection. we investigated whether symptomatic e histolytica amebic colitis in hiv-infected persons requires higher doses or longer duration ... | 1996 | 18611760 |
| asymptomatic salmonella, shigella and intestinal parasites among primary school children in the eastern province. | the main objective of this study was to determine the prevalence of some potential entropathogens among primary school children. | 1995 | 23012224 |
| evaluation of an enzyme-linked immunoassay for the detection in serum of antibodies to entamoeba histolytica. | a commercially available enzyme-linked immunoassay (elisa) was compared to an indirect hemagglutination test (iha) for the detection of antibodies to entamoeba histolytica on 225 patients' serums. all of the 107 serums that had iha titers of < 32 (interpreted as excluding the presence of invasive amebiasis) were elisa negative. sixty-three of 68 (93%) serums that had iha titers of > or = 128 (interpreted as indicative of the presence of active or recent infection) were elisa positive. fifty seru ... | 1995 | 8565416 |
| isolation and axenization of two entamoeba histolytica strains. | we describe the isolation and axenization of two e. histolytica strains, obtained from the stools of two patients with the clinical diagnosis of dysentery. we used pavlova's medium for initial polixenic culture, and tyi-s-33 (diamond's) medium for monoxenic and axenic cultures. in order to eliminate the microorganism contaminating the stools the following antibiotics were used: penicillin, streptomycin, ampicillin, chloramphenicol, kanamycin, nistatin, ceftriaxone and amphoterycin b. both strain ... | 1995 | 8566669 |
| rapid diagnosis of entamoeba infection by using entamoeba and entamoeba histolytica stool antigen detection kits. | humans are infected by two morphologically identical species of entamoeba: entamoeba histolytica causes amebic colitis and liver abscess, and entamoeba dispar is noninvasive. several weeks of culture and isoenzyme (zymodeme) analysis are required to differentiate e. histolytica from e. dispar. here we report a field trial of commercial antigen detection kits designed to rapidly detect and differentiate e. histolytica from e. dispar in stool specimens. stool specimens from 202 patients with diarr ... | 1995 | 8567882 |
| prevalence of enteric parasitic infection among hiv-infected attendees of an inner city aids clinic. | one hundred individuals confirmed to be infected with the human immunodeficiency virus (hiv), volunteered to participate in a pilot study comparing clinical status and enteric parasite burden with race, age, cd4 levels, risk factors and sexual practice. the prevalence of enteric parasites was 55 (55%) for giardia lamblia, 6 (6%) for cryptosporidium, 10 (10%) for isospora belli and 3 (3%) for entamoeba histolytica. there was no associations between demographic variables and the presence of parasi ... | 1995 | 8574144 |
| molecular analysis of two hexokinase isoenzymes from entamoeba histolytica. | the zymodemes, electrophoretic patterns of hexokinase, phosphoglucomutase and glucose phosphate isomerase isoenzymes, have been widely used to determine the pathogenicity of entamoeba histolytica isolates. although pathogenic and nonpathogenic forms of e. histolytica differ clearly in sequences of many homologous genes, a conversion between pathogenic and nonpathogenic zymodemes has been reported by several laboratories. to approach the question what might be the basis for the observed conversio ... | 1995 | 8577326 |
| identification and analysis of the start site of ribosomal rna transcription of entamoeba histolytica. | in this article we report the identification of the start site of ribosomal rna transcription unit of the enteric parasite e. histolytica. we cloned the upstream region of the ribosomal rna and we defined the 5' boundary of the transcription unit with nuclear run-on assays. we report that ribosomal transcription starts 2447 bp upstream the ssu ribosomal gene, at an adenosine residue. this data was supported both by s1 mapping and by primer extension analysis; that the mapped site was indeed the ... | 1995 | 8577327 |
| transcription of protein-coding genes in entamoeba histolytica is insensitive to high concentrations of alpha-amanitin. | 1995 | 8577336 | |
| kinetic mechanism of pyrophosphate-dependent phosphofructokinase from giardia lamblia. | the steady-state kinetics of the reaction catalyzed by inorganic-pyrophosphate-dependent d-fructose-6-phosphate 1-phosphotransferase from giardia lamblia have been investigated. the reactants for the forward and reverse reactions were the mg-chelated complexes of pyrophosphate (ppi) and pi. uncomplexed ligands were not substrates. in the direction of phosphorylation of fructose-6-phosphate (f6p), initial velocity double-reciprocal plots for both ppi and f6p were intersecting suggesting sequentia ... | 1995 | 8577346 |
| 'the tropics in our bathroom': chronic diarrhoea after return from the tropics. | interest in imported tropical diseases has increased with the rising number of travellers to the tropics. this is especially true in the case of tropical gastroenterologic disorders. the causative organisms of chronic diarrhoea are different from those causing acute diarrhoea. bacteria are relatively unusual; parasites, e.g. entamoeba histolytica or giardia lamblia or an opportunistic parasitic infestation associated with an hiv infection are more likely. furthermore, non-infectious causes, such ... | 1995 | 8578232 |
| intestinal opportunistic parasites among different groups of immunocompromised hosts. | the present study was designed to find out the prevalence of different intestinal opportunistic parasites in different groups of immunocompromised hosts; patients suffering from malignancy with or without immuno-suppressive drugs, or with chronic renal failure, or diabetes mellitus, or subjects under cortisone therapy. examination of stool samples collected from 427 immunocompromised hosts revealed the detection of intestinal opportunistic parasites in 98 samples, with a prevalence of 23%. infec ... | 1995 | 8586866 |
| chronic diarrhea among adults in kigali, rwanda: association with bacterial enteropathogens, rectocolonic inflammation, and human immunodeficiency virus infection. | one hundred patients with chronic diarrhea were seen in the department of internal medicine at the centre hospitalier de kigali, rwanda; stool and/or rectal swab culture was performed for these patients, and they underwent rectoscopy and serological testing for human immunodeficiency virus type 1 (hiv-1). enteropathogenic bacteria were isolated from 39 (39%) of the patients: shigella species (22 of 100 patients tested), non-typhi salmonella (11/100), aeromonas species (5/60), and campylobacter s ... | 1995 | 8589155 |
| [interleukin-8 gene expression in the human colon epithelial cell line, ht-29, exposed to entamoeba histolytica]. | the protozoan parasite, entamoeba histolytica, is one of major causative agents of intestinal disease all over the world. in acute experimental infection, the early host response to e. histolytica is characterized by an infiltration of neutrophils. however, the chemotactic signal for this response is not well known. based on the finding that human epithelial cells produce the potent neutrophil chemoattractant and activator, interleukin-8 (il-8), il-8 gene expression was examined thoroughly in hu ... | 1995 | 8591014 |
| axenization of entamoeba histolytica, a korean strain ys-27. | in the present communication, ys-27, a korean strain of entamoeba histolytica is described for the isolation and establishment of axenic cultivation. e. histolytica, designated as strain "ys-27", was isolated from the pus of a hepatic abscess obtained from a 72 year old inpatient of august 10, 1969. specimens, were obtained by needle aspiration, inoculated immediately and weekly cultured in a modified diphasic medium at 37 degrees c. strain ys-27 had been maintained for more than 15 years by wee ... | 1995 | 8591018 |
| [parasitic diseases of the liver]. | the liver seems to be very attractive for many parasites which may either inhabit the organ or pass through during their normal development or may be carried to the liver leading to destruction there. malaria parasites have a short developmental stage in the hepatocytes, but they do not damage the organ. for the leishmanias (leishmania donovani complex) are the kupffer cells are the target cells causing visceral leishmaniasis or kala azar. nematodes of the ascarid group have a special affinity f ... | 1995 | 8600687 |
| analysis of the 170-kda lectin gene promoter of entamoeba histolytica. | the promoter region driving the gene for the 170-kda heavy subunit of the entamoeba histolytica galactose-inhibitable lectin was analysed by transient transfection using the chloramphenicol acetyltransferase gene as reporter. s1 mapping confirmed our previous notion that the promoter is located within a 1.35-kb intergenic sequence preceding the structural lectin gene. transcripts derived from the chloramphenicol acetyltransferase gene of transfected trophozoites were found to be polyadenylated a ... | 1995 | 8538679 |
| free radical detoxification in giardia duodenalis. | non-denaturing polyacrylamide gels were used to analyse superoxide dismutase (sod), catalase, peroxidase, nadh oxidase and nadh peroxidase in the microaerophilic protozoan parasite giardia duodenalis. a cytosolic h2o-producing nadh oxidase and membrane-associated nadh peroxidase were readily detected from g. duodenalis. in all giardia strains investigated the nadh oxidase was present in high levels (1.2-2 u (mg protein)-1). using the same technique, nadh oxidase activity was also detected in the ... | 1995 | 8538699 |
| zoonotic intestinal parasites of hamadryas baboons papio hamadryas in the western and northern regions of saudi arabia. | six hundred and thirty-three faecal samples were randomly collected and examined for ova and cysts of intestinal parasites from five groups of hamadryas baboons of different population densities, with different human contact and in different ecological conditions (al-baha, turabah and al-taif in south-western and al-rihat and al-akhal in north-western saudi arabia). nine parasites were recorded (giardia lamblia, entamoeba histolytica, escherichia coli, balantidium coli, enterobius sp., trichuris ... | 1995 | 8544227 |
| carbohydrate epitopes of entamoeba histolytica cell surface glycoproteins are major targets of the human humoral response. | the antigens of entamoeba histolytica recognized by antibodies in 11 individual sera from patients treated for amebic liver abscess were determined both by immunoprecipitation of metabolically-radiolabeled whole trophozoite proteins and by immunoblotting.collectively, twenty-s even antigens ranging from 167 to 21 kda were detected in immunoblots of whole trophozoite extracts; eight of these were recognized by all tested patient sera. immunoprecipitation studies also revealed a complex amebic ant ... | 1995 | 8546039 |
| the seroprevalence of ten zoonoses in two villages of crete, greece. | the seroprevalence and incidence of 10 zoonoses due to rickettsia typhi, r. conorii, coxiella burnetii, burcella sp., borrelia sp., toxoplasma sp., leishmania sp., entamoeba histolytica, echinococcus granulosus and fasciola hepatica were studied in an animal husbandry and a farming village in crete, greece. the serum conversion incidence of each infectious agent was determined by testing 2 blood samples, collected in 1985 and in 1987. the surveillance was conducted using detailed transparent map ... | 1995 | 8549708 |
| the relationship of water sources and other determinants to prevalence of intestinal protozoal infections in a rural community of saudi arabia. | a study was carried out in a rural area of asir region in southwest of saudi arabia where information on the types of domestic water supply and sociodemography in relation to intestinal protozoal infections were obtained from 1417 inhabitants (81.4% of the total) of nine villages. stool samples were collected from the study population and examined for intestinal parasites. overall, the prevalence rates of infection with the common water-borne parasites, giardia lamblia and entamoeba histolytica ... | 1995 | 8550870 |
| the association of the amoebic colitis and chronic ulcerative colitis. | three patients with idiopathic ulcerative colitis and infection of entamoeba histolytica were reviewed. they represented three different relationships between the two conditions, vis asymptomatic carrier, coexisting infection and superinfection causing exacerbation of colitis. a two-way relationship between amoebic colitis and ulcerative colitis has been discussed and the possible mechanisms in the literature have been reviewed. in patients with a short history of diarrhoea and in tropical count ... | 1995 | 8553098 |
| entamoeba histolytica axenic growth improvement by ox bile. | axenic entamoeba histolytica cultures, grown in pehps medium, showed increasing yields and growth velocity when added with 0.03 to 0.6 g/l gallbladder ox bile, while higher doses were toxic for amebas. the highest density (505 +/- 29 x 10(3) trophozoites/ml) and shortest duplication time (dt = 10.74 +/- 0.2 h) corresponded to cultures added with 0.24 g bile/l. their yields were 1.74 and 3.34 times higher, respectively, than those which were reached by the non-added pehps controls and by growth o ... | 1995 | 8555742 |
| prevalence of antibodies against entamoeba histolytica in mexico measured by elisa. | the prevalence of antibodies against entamoeba histolytica was studied in the mexican population using an immunoenzyme assay in solid phase (elisa) and semiautomatic equipment. the antigen was a mixture of membrane proteins obtained by triton x-100 extraction from an axenic culture of entamoeba histolytica hm1-imss. the method was standardized by comparing serum samples from amoebic liver abscess patients with healthy volunteers. from the 60,538 samples supplied by the national seroepidemiology ... | 1995 | 8557086 |
| the basic isoform of profilin in pathogenic entamoeba histolytica. cdna cloning, heterologous expression, and actin-binding properties. | in the human parasite entamoeba histolytica, components of the cytoskeleton are involved in the pathogenicity by their contribution to immune evasion by antibody capping and shedding. in this study, we focus on profilin as a central regulatory component of the cytoskeleton. profilin was isolated from trophozoites of the pathogenic e. histolytica strain sfl-3, and partial amino acid sequences were used to devise a probe for isolating a profilin cdna. the deduced complete primary structure was div ... | 1995 | 8521867 |
| [amebic liver abscess: review of 13 cases]. | amebian hepatic abscess (aha) is the most frequent extraintestinal localization of infection by entamoeba histolytica. despite being a disease mainly of tropical zones, it is currently also observed with higher frequency in spain. thirteen cases of aha diagnosed in the authors' hospital over the last 11 years were retrospectively analyzed comparing the data in this series with that of other published series and undertaking a review of this subject. the diagnosis of aha was established on the bas ... | 1995 | 8523930 |
| intestinal parasites in school food handlers in the city of uberlândia, minas gerais, brazil. | in order to verify the presence of intestinal parasites in food handlers, stool samples were collected from 104 cooks and their helpers that were working in food preparation in 20 public elementary schools, in various areas of the city of uberlândia, minas gerais, brazil. the samples were collected during the months of november and december, 1988, in plastic flasks containing a 10% formaldehyde solution and processed by the hoffmann, pons & janer method. the sediment was examined using triplicat ... | 1995 | 8525262 |
| influence of bacteria upon cytopathic effect and erythrophagocytosis of different axenic strains of entamoeba histolytica. | at this moment, the duality of species suggested for e. histolytica is being considered for discussion. in order to contribute to settling this question, we investigated the possibility of conversion of avirulent ameba to virulent ones, as well as, the possibility of increasing virulence of virulent strains, by means of association with bacteria. five strains of e. histolytica were employed, two of them regarded as avirulent and three virulent ones. amebas were associated with the bacteria esche ... | 1995 | 8525263 |
| opportunistic and non-opportunistic parasites in hiv-positive and negative patients with diarrhoea in tanzania. | a survey on intestinal parasites in a rural area of tanzania revealed the presence of eight protozoa and seven helminths in 287 subjects (81.8%). the prevalence of entamoeba histolytica and ascaris lumbricoides was higher in hiv-negative than in hiv-positive patients (p < 0.01; p < 0.04) (25.1% and 12.5% for e. histolytica; 10.5% and 3.7% for a. lumbricoides). on the other hand, cryptosporidium parvum, isospora belli and strongyloides stercoralis prevalence was higher in hiv-positive than in hiv ... | 1995 | 8525281 |
| prevalence of intestinal parasitic infections among asian female house keepers in abha district, saudi arabia. | a group of 5,518 female asian house keepers working in abha district of saudi arabia was examined (1990 through 1992) to determine the prevalence of intestinal parasitic infections. they came from indonesia, sri lanka, philippines and thailand. fresh stool specimens were obtained in special containers and examined by light microscopy of wet smears in normal saline and lugoll's iodine solution within one hour of collection. the study revealed an overall prevalence of 46.5% which was higher than t ... | 1995 | 8525400 |
| entamoeba histolytica adherence: inhibition by iga monoclonal antibodies. | 1995 | 8526018 | |
| a novel transcribed repeat element from entamoeba histolytica. | we have identified an unusual 0.55-kb dna repeat element specific to entamoeba histolytica (eh) which we call interspersed element (ie). the ie is a common feature in independently isolated genomic and cdna fragments. hybridization of labeled ie sequences to trophozoite dna, rna and first-strand cdna prepared from poly(a)-enriched mrna indicate that the ie are reiterated about 500 times per eh trophozoite and that one or more can be found as rna transcripts. these features and the degree of cons ... | 1995 | 8529889 |
| viral diarrhoea in a rural coastal region of karnataka india. | a total of 106 children below 5 years of age admitted to the kasturba medical college hospital manipal karnataka (south india) were investigated over a period of 6 months to determine the aetiological role of viruses in acute diarrhoea. viral aetiological agents isolated were rotaviruses in 12 (11 per cent) cases, adenoviruses in 3 (3 per cent) cases, coronavirus and astroviruses in two (2 per cent) cases each. non-viral isolates were cryptosporidium and salmonella typhimurium in two cases each, ... | 1995 | 8531264 |
| amoebicidal compounds from medicinal plants. | a series of the natural constituents with amoebicidal activity isolated from several medicinal plants is shown. a list of the medicinal plants potentially active as amoebicide and/or against dysentery also is demonstrated. the present data grouping of the natural compounds and medicinal plants can be an important source of information for the selection of research plant material by the investigators interested in the discovery of new biologically active compounds as amoebicide. | 1995 | 8532364 |
| evaluation of three serological tests for the detection of antiamebic antibodies applied to sera of patients from an area endemic for amebiasis. | two enzyme immuno assays based on a single recombinant entamoeba histolytica antigen (p1-eia) or soluble e. histolytica extract (sa-eia) as well as a latex agglutination test using an e. histolytica membrane fraction (m-la) were evaluated for its use to detect anti-amebic serum antibodies in patients from durban, south africa, an area endemic for amebiasis. in a previous study, all three test systems were found to be reliable in terms of sensitivity and specificity when applied to sera of europe ... | 1995 | 8533021 |
| [clinico-epidemiological implications of autochthonous infection caused by entamoeba histolytica]. | 1995 | 8534547 | |
| prevalence of intestinal parasites in saudi children: a community-based study. | a community-based study was undertaken to determine the prevalence and types of intestinal parasites in the pediatric population of al-baha region, saudi arabia. stool samples were randomly collected from 19,939 children of whom 4208 (21.1%) were found to harbour intestinal parasites. the most affected age group was 5-9 years and the sex distribution was almost equal in all age groups. specific prevalence rates were giardia lamblia 9 per cent, entamoeba histolytica 5 per cent, hymenolepis nana 2 ... | 1995 | 7723130 |
| the primary structure of an entamoeba histolytica enolase. | 1995 | 7723780 | |
| [underestimation of intestinal protozoa as a cause of diarrhea in family practice]. | 1995 | 7723885 | |
| entamoeba histolytica: increase of enterotoxicity and of 53- and 75-kda cysteine proteinases in a clone of higher virulence. | we compared the enterotoxicity and cysteine proteinases (cp) of the low-virulence entamoeba histolytica hm1 strain with the highly virulent 1659 clone, derived from hm1 by hamster liver passages. enterotoxicity of 50,000 freeze-thawed trophozoites was determined on 0.28-cm2 intestinal segments mounted in ussing chambers; cp activity of nonidet-p40 amebal lysates was assayed by gelatin-sodium dodecyl sulfate-polyacrylamide gel electrophoresis and carbobenzoxy-l-arginine-l-arginyl-p-nitroaniline, ... | 1995 | 7729472 |
| entamoeba histolytica: induction and isotype analysis of antibody producing cell responses in peyer's patches and spleen after local and systemic immunization in male and female mice. | the purpose of this work was to determine if anti-amebic antibody producing cell responses could be elicited in peyer's patches and spleen in mice locally or systemically immunized with glutaraldehyde-fixed trophozoites of entamoeba histolytica (gft). the animals were inoculated with either a single or four doses of gft via intragastric, rectal, and intraperitoneal routes. the anti-amebic antibody producing cell responses were analyzed by a spot forming cell assay (elispot). the kinetics of anti ... | 1995 | 7729489 |
| inositol(1,3,4,5) tetrakisphosphate plays an important role in calcium mobilization from entamoeba histolytica. | calcium release from internal stores of entamoeba histolytica, a parasitic protozoan, was observed by measuring fluorescence of fura-2. emptying of inositol(1,4,5)trisphosphate (ins(1,4,5)p3)-sensitive calcium pools in permeabilized e. histolytica did not significantly affect subsequent calcium release by inositol(1,3,4,5)tetrakis-phosphate (ins(1,3,4,5)p4). similarly, prior depletion of ins(1,3,4,5)p4-sensitive stores did not have any influence on subsequent calcium release by ins(1,4,5)p3. the ... | 1995 | 7729520 |
| entamoeba histolytica autochthonous isolates from mentally retarded italian patients. | a total of 77 mentally retarded male inpatients residing in a psychiatric institution in northern italy were screened for the presence of stool parasites, entamoeba histolytica particularly. parasitological stool examination showed entamoeba spp. (e. histolytica and/or e. dispar) in 26 cases (33.7%). in vitro culture on robinson's medium was positive in 16 cases (61.1%); in 11 cases we could stabilize and clone the isolates and proceed to electrophoretic assays. in all cases, patterns of pathoge ... | 1995 | 7731923 |
| cyclic nucleotide changes induced in human leukocytes by a product of axenically grown entamoeba histolytica that inhibits human monocyte locomotion. | pulse exposure of human mononuclear phagocytes to the monocyte locomotion-inhibitory factor produced by entamoeba histolytica (i.e., the 369- to 765-da chromatographic fraction obtained from the supernatant fluid of axenically grown e. histolytica) led to a swift increase in the intracellular concentration of adenosine 3':5' cyclic monophosphate (camp). a weaker response was observed in human polymorphonuclear leukocytes, the locomotion of which, however, is not inhibited by this amebic factor. ... | 1995 | 7731924 |
| intestinal parasitic infections in the residents of an emigration camp in tijuana, mexico. | we examined stool specimens of the residents in the emigration camp in tijuana, mexico for helminth eggs or protozoan cysts with formalin-ethyl acetate concentration method in february and july 1992. out of 92 examined samples, number of positive was 49 (53.3%). while number of cumulative positive was 66 (71.7%). cysts of entamoeba coli (29.3%) giardia lamblia (9.8%), entamoeba histolytica (7.6%), and eggs of taenia spp. (6.5) were most frequently observed. filtered water supply and chemotherapy ... | 1995 | 7735788 |
| the antiamoebic effect of a crude drug formulation of herbal extracts against entamoeba histolytica in vitro and in vivo. | the antiamoebic effect of a crude drug formulation against entamoeba histolytica was studied. in the traditional system of medicine in india, the formulation has been prescribed for intestinal disorders. it comprises of five medicinal herbs, namely, boerhavia diffusa, berberis aristata, tinospora cordifolia, terminalia chebula and zingiber officinale. the dried and pulverized plants were extracted in ethanol together and individually. in vitro amoebicidal activity was studied to determine the mi ... | 1995 | 7739226 |
| entamoeba histolytica trophozoites induce an inflammatory cytokine response by cultured human cells through the paracrine action of cytolytically released interleukin-1 alpha. | infection with the protozoan parasite entamoeba histolytica results in a high mortality worldwide. to initiate infection, e. histolytica trophozoites in the bowel lumen penetrate the epithelium, and cause extensive lysis of host cells. the acute amebic lesions in animal models are characterized by infiltration with inflammatory cells, particularly neutrophils. the acute host response is likely important for determining whether the infection will spread systemically, but little is known regarding ... | 1995 | 7657801 |