Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| in-vitro antibacterial and cytotoxic activity of cobalt (ii), copper (ii), nickel (ii) and zinc (ii) complexes of the antibiotic drug cephalothin (keflin). | keflin (kefl) interacts with co(ii), cu(ii), ni(ii) and zn(ii) metal ions leading to complexes of the type m(kefl)2cl2 and m(kefl)cl2, which have been characterized by physicochemical and spectroscopic methods. magnetic moment, ir, electronic spectral and elemental analyses data suggest that keflin behaves tridentately forming octahedral or trigonal bipyramidal complexes with the metal ions mentioned above. the new compounds have been screened in-vitro for antibacterial and cytotoxic activity ag ... | 2005 | 16335054 |
| [structural and functional aspects of bacterial membranes and liposomes interactions]. | effect of exogenous lipids on the morphology and enzymatic activity of bacillus cereus b4368 membrane has been studied. specific character of different lipids action on the membrane enzyme activity was found. this peculiarity had been taken into account by using liposomes in biological systems. | 2005 | 16335239 |
| outbreak of cutaneous bacillus cereus infections among cadets in a university military program--georgia, august 2004. | although bacillus cereus is known mainly as an agent of food poisoning, other infections caused by this organism have been documented in immunocompromised patients, including sepsis, meningitis, pneumonia, and wound infections. certain populations are at increased risk for b. cereus infection, including cancer patients, neonates, intravenous drug users, and patients with a history of trauma, surgery, or catheterization. primary cutaneous disease attributed to b. cereus in immunocompetent persons ... | 2005 | 16340940 |
| stability of enterocin as-48 in fruit and vegetable juices. | enterocin as-48 is a candidate bacteriocin for food biopreservation. before addressing application of as-48 to vegetable-based foods, the interaction between as-48 and vegetable food components and the stability of as-48 were studied. enterocin as-48 had variable interactions with fruit and vegetable juices, with complete, partial, or negligible loss of activity. for some juices, loss of activity was ameliorated by increasing the bacteriocin concentration, diluting the juice, or applying a heat ... | 2005 | 16245711 |
| establishment of a novel multiplex pcr assay and detection of toxigenic strains of the species in the bacillus cereus group. | five different enterotoxins and one emetic toxin of bacillus cereus have been characterized. to amplify all of the enterotoxin and emetic-specific sequences of the species in the b. cereus group, a multiplex pcr with 12 primer pairs was established. in developing the assay method, a common terminal sequence at the 3' ends of all primers was chosen and a hot start taq polymerase was used to overcome primer dimer formation. the assay was successfully applied to analyze the toxigenic potential of 1 ... | 2005 | 16245717 |
| [study of real-time pcr assays for rapid detection of food-borne pathogens]. | a duplex real-time sybr green lightcycler pcr (lc-pcr) assay with dna extraction using qiaamp dna stool minikit was evaluated for detection of 8 of 19 species of food-borne pathogens, including plesiomonas shigelloides, providencia alcalifaciens, in five stool specimens. the time frame was within 2h or less. the protocol used the same lc-pcr with 22 pairs of specific primers. the rapid amplification and reliable detection of specific genes were determined by this lc-pcr assay from 10 cases of fo ... | 2005 | 16248373 |
| immunoassay and polymerase chain reaction techniques for detection of enterotoxigenic bacillus cereus. | to compare the reverse passive latex agglutination (rpla) and enzyme linked immunosorbent assay (elisa) techniques with a polymerase chain reaction (pcr) for detection of enterotoxigenic bacillus cereus. | 2005 | 16261920 |
| optimal sampling time after preparation of platelet concentrates for detection of bacterial contamination by quantitative real-time polymerase chain reaction. | a universal quantitative real-time polymerase chain reaction (pcr), based on bacterial 16s rdna, to detect bacterial contamination of platelet concentrates (pcs), was developed previously and compared with automated culturing. in the present study, this real-time pcr method was evaluated to determine the optimal sampling time for screening of bacterial contamination in pcs. | 2005 | 16262753 |
| bacillus cereus var. toyoii and saccharomyces boulardii increased feed efficiency in broilers infected with salmonella enteritidis. | 1. the effect on feed efficiency of two probiotics, one prepared with saccharomyces boulardii and the other with bacillus cereus var. toyoii, was tested in broilers infected with salmonella enteritidis. 2. one-day-old chicks were divided at random into three groups and fed commercial feed devoid of antibiotics: group 1 was fed with non-supplemented feed, group 2 was supplemented with s. boulardii and group 3 with b. cereus. at 14 d of age the animals were challenged by the oral route with 1 x 10 ... | 2005 | 16268108 |
| comparison of minisatellite polymorphisms in the bacillus cereus complex: a simple assay for large-scale screening and identification of strains most closely related to bacillus anthracis. | polymorphism of five tandem repeats that are monomorphic in bacillus anthracis was investigated in 230 isolates of the b. cereus group and in 5 sequenced b. cereus genomes in search for markers allowing identification of b. cereus and b. thuringiensis strains most closely related to b. anthracis. using this multiple-locus variable number of tandem repeat analysis (mlva), a cluster of 30 strains was selected for further characterization. eventually, six of these were characterized by multilocus s ... | 2005 | 16269689 |
| photodynamic inactivation of bacillus spores, mediated by phenothiazinium dyes. | spore formation is a sophisticated mechanism by which some bacteria survive conditions of stress and starvation by producing a multilayered protective capsule enclosing their condensed dna. spores are highly resistant to damage by heat, radiation, and commonly employed antibacterial agents. previously, spores have also been shown to be resistant to photodynamic inactivation using dyes and light that easily destroy the corresponding vegetative bacteria. we have discovered that bacillus spores are ... | 2005 | 16269726 |
| investigations on bacteria as a potential biological control agent of summer chafer, amphimallon solstitiale l. (coleoptera: scarabaeidae). | studying the bacteria of hazardous insects allows the opportunity to find potentially better biological control agents. therefore, in this study, bacteria from summer chafer (amphimallon solstitiale l., coleoptera: scarabaeidae) we isolated and identified the insecticidal effects of bacteria isolated from a. solstitiale and melolontha melolontha l. (common cockchafer, coleoptera: scarabaeidae) and the mixtures of these bacterial isolates were investigated on a. solstitiale larvae. crystals from ... | 2005 | 16273040 |
| proximity ligation assays with peptide conjugate 'burrs' for the sensitive detection of spores. | the proximity ligation assay (pla) has previously been used for the sensitive and specific detection of single proteins. in order to adapt pla methods for the detection of cell surfaces, we have generated multivalent peptide-oligonucleotide-phycoerythrin conjugates ('burrs') that can bind adjacent to one another on a cell surface and be ligated together to form unique amplicons. real-time pcr detection of burr ligation events specifically identified as few as 100 bacillus anthracis, 10 bacillus ... | 2005 | 16237122 |
| the autolytic phenotype of the bacillus cereus group. | to determine the autolytic phenotype of five species in the bacillus cereus group. | 2005 | 16238737 |
| isolation and identification of nitrogen-fixing bacilli from plant rhizospheres in beijing region. | to isolate and identify nitrogen-fixing bacilli from the plant rhizospheres in beijing region of china. | 2005 | 16238759 |
| genome sequence and gene expression of bacillus anthracis bacteriophage fah. | fah, a lytic bacteriophage of bacillus anthracis, is used widely in the former soviet union to identify anthrax bacteria. here, we present the analysis of a 37,974 bp sequence of the fah genome and examine gene expression of the phage in a model host, bacillus cereus. half of the fah genome contains genes coding for structural proteins and host lysis functions in an arrangement typical of syphoviridae. the other half of the genome contains genes coding for enzymes of viral genome replication and ... | 2005 | 16226766 |
| bacillus cereus produces several nonproteinaceous insecticidal exotoxins. | bacillus cereus is mainly known as a human food-borne opportunistic pathogen. here, we used biological assays and hplc to investigate the ability of b. cereus to produce insecticidal exotoxins during the stationary growth phase. none of the 575 b. cereus strains screened produced detectable levels of beta-exotoxin i, a small, heat-stable insecticidal nucleotide analogue. however, six out of a subset of 270 b. cereus strains produced several small, nonproteinaceous insecticidal exotoxins differen ... | 2005 | 16214163 |
| characterization of calcium-activated bifunctional peptidase of the psychrotrophic bacillus cereus. | the protease purified from bacillus cereus jh108 has the function of leucine specific endopeptidase. when measured by hydrolysis of synthetic substrate (n-succinyl-ala-ala-pro-leu-p-nitroanilide), the enzyme activity exhibited optimal activity at ph 9.0, 60 degrees c. the endopeptidase activity was stimulated by ca++, co++, mn++, mg++, and ni++, and was inhibited by metal chelating agents such as edta, 1,10-phenanthroline, and egta. addition of serine protease inhibitor, pmsf, resulted in the el ... | 2005 | 15995640 |
| the cereulide genetic determinants of emetic bacillus cereus are plasmid-borne. | 2005 | 16000702 | |
| effect of boiling and roasting on the fermentation of soybeans into dawadawa (soy-dawadawa). | soybeans which had initially been dehulled by either boiling (boiled/dehulled) or roasting (roasted/dehulled) before peeling, were cooked and fermented into dawadawa, a traditional food condiment. the micropopulation, enzymatic activities, proximate composition, amino acid, and aroma profiles of the two types of soybean dawadawa were evaluated during fermentation. only minor differences were found in the microbial profiles of the two types of soy-dawadawa. although boiled/dehulled soy-dawadawa i ... | 2005 | 16002169 |
| the possibility of discriminating within the bacillus cereus group using gyrb sequencing and pcr-rflp. | based on a combination of pcr and restriction endonuclease (re) digestion (pcr-re digestion), we have examined the possibility of differentiating members of the bacillus cereus group. fragments of the gyrb gene (362 bp) from pure cultures of 12 b. cereus, 25 b. thuringiensis, 25 b. mycoides and two b. anthracis strains were amplified and subsequently digested with sau3a1. furthermore, a majority of the amplicons were sequenced directly to verify the pcr-re results. the results obtained suggest t ... | 2005 | 16005534 |
| systematic determination of the mosaic structure of bacterial genomes: species backbone versus strain-specific loops. | public databases now contain multitude of complete bacterial genomes, including several genomes of the same species. the available data offers new opportunities to address questions about bacterial genome evolution, a task that requires reliable fine comparison data of closely related genomes. recent analyses have shown, using pairwise whole genome alignments, that it is possible to segment bacterial genomes into a common conserved backbone and strain-specific sequences called loops. | 2005 | 16011797 |
| effect of heat treatment of milk on activation of bacillus spores. | the quality and shelf life of fluid milk products are dependent on the amount and type of microorganisms present following pasteurization. this study evaluated the effects of different pasteurization processes on the microbial populations in fluid milk. the objective was to determine whether certain pasteurization processes lead to an increase in the amount of bacteria present in pasteurized milk by activating bacillus spores. samples of raw milk were collected on the day of arrival at the dairy ... | 2005 | 16013392 |
| [expression and bioactive characterization of bacteriophage lysin gene of bacillus anthracis in escherichia coli]. | the lysin gene of bacillus anthracis-diagnosing bacteriophage, obtained by pcr amplification,was cloned into the escherichia coli exepression vector pet22b which has been cleaved by ecor i and nde i. the recombinant vector pet22b-gamma lysin was verified to be correctly constructed by pcr, sequencing and enzyme digestion, and highly expressed in e. coli bl21 (de3), which accounted for about 40 percent of total protein in e. coli bl21 (de3), while in the 5l fermentor the expression level reached ... | 2005 | 16013478 |
| [multiplex amplification test system for the identification and differentiation of bacillus anthracis]. | the multiplex amplification test system for the identification of bacillus anthracis with primers to plasmid cya (px01), capc (px02) genes and chromosomal sap gene were developed. the primers to sap gene were selected by the authors and, after being tested on 72 microbial strains of the genus bacillus, proposed as more specific in comparison with the known primers to chromosomal locus ba 813. the proposed test system permitted the simultaneous identification of b. anthracis of all plasmid varian ... | 2005 | 16028518 |
| contribution of exsfa and exsfb proteins to the localization of bcla on the spore surface and to the stability of the bacillus anthracis exosporium. | spores of bacillus anthracis, the etiological agent of anthrax, and the closely related species bacillus cereus and bacillus thuringiensis, possess an exosporium, which is the outermost structure surrounding the mature spore. it consists of a paracrystalline basal layer and a hair-like outer layer. to date, the structural contribution of only one exosporium component, the collagen-like glycoprotein bcla, has been described. it is the structural component of the hair-like filaments. here, we desc ... | 2005 | 16030205 |
| unusual group ii introns in bacteria of the bacillus cereus group. | a combination of sequence and structure analysis and reverse transcriptase pcr experiments was used to characterize the group ii introns in the complete genomes of two strains of the pathogen bacillus cereus. while b. cereus atcc 14579 harbors a single intron element in the chromosome, b. cereus atcc 10987 contains three introns in the chromosome and four in its 208-kb pbc10987 plasmid. the most striking finding is the presence in b. cereus atcc 10987 of an intron [b.c.i2(a)] located on the reve ... | 2005 | 16030238 |
| biodegradation of imazapyr in typical soils in zhejiang province, china. | the degradation of imazapyr in non-sterile and sterile soils from four sampling sites in zhejiang, china was studied. the results showed that the half-lives of imazapyr in non-sterile soils were in the range of 30 to 45 d, while 81 to 133 d in sterile (by autoclaving) soils. it means the rate constants of imazapyr under non-sterile conditions were 2.3-4.4 times faster than that under sterile (by autoclaving) conditions, evidently indicating that the indigenous microorganisms in soil play an impo ... | 2005 | 16158586 |
| investigations on anti-aspergillus properties of bacterial products. | to investigate the anti-aspergillus properties of bacterial products. | 2005 | 16162136 |
| naphthalene degradation and biosurfactant activity by bacillus cereus 28bn. | biosurfactant activity and naphthalene degradation by a new strain identified as bacillus cereus 28bn were studied. the strain grew well and produced effective biosurfactants in the presence of n-alkanes, naphthalene, crude oil and vegetable oils. the biosurfactants were detected by the surface tension lowering of the medium, thin layer chromatography and infrared spectra analysis. with (2%) naphthalene as the sole carbon source, high levels of rhamnolipids at a concentration of 2.3 g 1(-1) were ... | 2005 | 16163832 |
| identification of the bacillus anthracis (gamma) phage receptor. | bacillus anthracis, a gram-positive, spore-forming bacterium, is the etiological agent of anthrax. it belongs to the bacillus cereus group, which also contains bacillus cereus and bacillus thuringiensis. most b. anthracis strains are sensitive to phage gamma, but most b. cereus and b. thuringiensis strains are resistant to the lytic action of phage gamma. here, we report the identification of a protein involved in the bacterial receptor for the gamma phage, which we term gamr (gamma phage recept ... | 2005 | 16166537 |
| a comparative study of bacillus cereus, bacillus thuringiensis and bacillus anthracis extracellular proteomes. | bacillus cereus, bacillus thuringiensis and bacillus anthracis are closely related species that share a similar genetic background but occupy different ecological niches. virulence plasmids bearing genes coding for toxins, may explain, at least partly, this specialization. we have compared by 2-de in the early stationary phase of growth the extracellular proteomes of three strains of these species that have lost their virulence plasmids. proteins expected to be secreted or to belong to the cell ... | 2005 | 16167365 |
| characterization of microorganisms in argentinean honeys from different sources. | seventy polyfloral honeys including commercial samples obtained from supermarkets, harvested from apiaries and purchased in bulk were initially examined for total antibacterial activity. from each sample, numbers of aerobic mesophilic bacteria, total coliforms, moulds and yeasts were determined and the presence of salmonella spp., shigella spp., clostridium sulfite-reducers, paenibacillus larvae and bacillus spp. was investigated. moisture content, ph and total acidity were also determined for a ... | 2005 | 16169624 |
| deletion of sigb in bacillus cereus affects spore properties. | in bacillus cereus and other gram-positive bacteria the alternative sigma factor sigma(b) is an important regulator of the stress response. deletion of the sigb gene generally leads to a stress-sensitive phenotype of vegetative cells. in this study, we describe the effect of the deletion of the sigb gene in b. cereus on spore properties. in particular, spores of the sigb deletion mutant showed a defect in germination upon exposure to the germinants alanine and inosine. | 2005 | 16171954 |
| mimicking natural evolution in metallo-beta-lactamases through second-shell ligand mutations. | metallo-beta-lactamases (mbls) represent the latest generation of beta-lactamases. the structural diversity and broad substrate profile of mbls allow them to confer resistance to most beta-lactam antibiotics. to explore the evolutionary potential of these enzymes, we have subjected the bacillus cereus mbl (bcii) to a directed evolution scheme, which resulted in an increased hydrolytic efficiency toward cephalexin. a systematic study of the hydrolytic profile, substrate binding, and active-site f ... | 2005 | 16172409 |
| prevalence of enterotoxigenic bacillus cereus and its enterotoxins in milk and milk products in and around nairobi. | to determine the prevalence of enterotoxigenic bacillus cereus (b. cereus) and enterotoxins in milk and milk products. | 2005 | 16175777 |
| [microbiologic evaluation of enteral diets handled in a hospital]. | to evaluate the microbiological quality of open enteral feeding system manipulated in a specialized hospital in oncology in the city of natal-rn. | 2005 | 16186972 |
| recurrent bacteraemia by 2 different bacillus cereus strains related to 2 distinct central venous catheters. | a 14-y-old girl with osteosarcoma developed 3 episodes of catheter-related bacteraemia by bacillus cereus. after removal of the first and insertion of a second hickman catheter, further episodes of b. cereus bacteraemia occurred. pfge analysis revealed that bacteraemic episodes related to each catheter were caused by a distinct b. cereus strain. | 2005 | 16191899 |
| bacillus cereus dna topoisomerase i and iiialpha: purification, characterization and complementation of escherichia coli topoiii activity. | the bacillus cereus genome possesses three type ia topoisomerase genes. these genes, encoding dna topoisomerase i and iiialpha (bctopo i, bctopo iiialpha), have been cloned into t7 rna polymerase-regulated plasmid expression vectors and the enzymes have been overexpressed, purified and characterized. the proteins exhibit similar biochemical activity to their escherichia coli counterparts, dna topoisomerase i and iii (ectopo i, ectopo iii). bctopo i is capable of efficiently relaxing negatively s ... | 2005 | 16192570 |
| cereulide-producing strains of bacillus cereus show diversity. | producers of cereulide, the emetic toxin of bacillus cereus, are known to constitute a specific subset within this species. we investigated physiological and genetic properties of 24 strains of b. cereus including two high cereulide producers (600-1,800 ng cereulide mg(-1) wet weight biomass), seven average producers (180-600 ng cereulide mg(-1) wet weight biomass), four low cereulide producers (20-160 ng cereulide mg(-1) wet weight biomass) and 11 non-producers representing isolates from food, ... | 2005 | 16195898 |
| characterization of sporulation histidine kinases of bacillus anthracis. | the initiation of sporulation in bacillus species is regulated by the phosphorelay signal transduction pathway, which is activated by several histidine sensor kinases in response to cellular and metabolic signals. comparison of the protein components of the phosphorelay between bacillus subtilis and bacillus anthracis revealed high homology in the phosphorelay orthologs of spo0f, spo0b, and spo0a. the sensor domains of sensor histidine kinases are poorly conserved between species, making ortholo ... | 2005 | 16199567 |
| deletion of the sigb gene in bacillus cereus atcc 14579 leads to hydrogen peroxide hyperresistance. | the sigb gene of bacillus cereus atcc 14579 encodes the alternative sigma factor sigma(b). deletion of sigb in b. cereus leads to hyperresistance to hydrogen peroxide. the expression of kata, which encodes one of the catalases of b. cereus, is upregulated in the sigb deletion mutant, and this may contribute to the hydrogen peroxide-resistant phenotype. | 2005 | 16204573 |
| in-vitro antibacterial, antifungal and cytotoxic activities of some coumarins and their metal complexes. | a series of new antibacterial and antifungal coumarin-derived compounds and their transition metal complexes [cobalt (ii), copper (ii), nickel (ii) and zinc (ii)] have been synthesized, characterized and screened for their in vitro antibacterial activity against escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, salmonella typhi, shigella dysenteriae, bacillus cereus, corynebacterium diphtheriae, staphylococcus aureus and streptococcus pyogenes bacterial strains ... | 2005 | 16206827 |
| purification of bovine milk lactoperoxidase and investigation of antibacterial properties at different thiocyanate mediated. | bovine lactoperoxidase (lpo) was purified with amberlite cg 50 h+ resin, cm sephadex c-50 ion-exchange chromatography, and sephadex g-100 gel filtration chromatography from skim milk. the activity of lactoperoxidase was measured by using 2.2-azino-bis (3-ethylbenzthiazoline-6 sulfonic acid) diammonium salt (abts) as a choromogenic substrate at ph 6.0. purification degree for the purified enzyme was controlled with sds-page and rz value (a412/a280). rz value for the purified lpo was 0.8. km value ... | 2005 | 16212035 |
| in-house validation of the bactec 9240 blood culture system for detection of bacterial contamination in platelet concentrates. | at present, only two commercially available automated culture systems are cleared by the fda for the purpose of quality control (qc) testing for bacterial contamination of platelet (plt) concentrates: the bact/alert blood culture system (biomérieux) and the pall ebds (pall corporation), both of which allow testing of leukoreduced apheresis as well as whole blood-derived plts. after the decision of the aabb to institute universal qc testing of plt concentrates for evidence of bacterial contaminat ... | 2005 | 15987359 |
| [identification of psychrotrophs syp-a2-3 producing cold-adapted protease from the no. 1 glacier of china and study on its fermentation conditions]. | the psychrotrophs syp-a2-3 producing the cold-adapted protease has been isolated from the bacterial samples collected from the no. 1 glacier of china and identified as bacillus cereus according to its morphological and physiochemical characteristics and 16s rdna gene sequence analysis. it could grow between 0 degree c and 38 degrees c while its optimal growth temperature was 25 degrees c and the optimal temperature for its protease production was 15 degrees c. the cold-adapted protease was ident ... | 2005 | 15989272 |
| effect of the implementation of haccp on the microbiological quality of meals at a university restaurant. | a study was conducted to evaluate the microbiological quality, including total mesophilic counts and markers of bacteriological hygiene, as indicator of food safety of three categories of the most consumed meals in a university restaurant, before and after implementation of the haccp system and personnel training. cold gastronomy products, cooked warm-served products, and cooked cold-served products were tested for bacterial contamination. throughout the experiment, 894 samples were examined for ... | 2005 | 15992308 |
| antagonistic activity against salmonella infection in vitro and in vivo for two lactobacillus strains from swine and poultry. | in this study, we isolated two lactobacillus strains, i.e., strain lap5 and lf33, from swine and poultry, respectively, and showed that both strains were acid as well as bile tolerant and were able to adhere to the cultured human intestinal cell lines, such as int-407 and caco-2 cells, and to the intestinal epithelium cells isolated from swine, poultry and mouse (balb/c). both of these lab strains were shown to inhibit the growth of escherichia coli, salmonella typhimurium, staphylococcus aureus ... | 2005 | 15992617 |
| cases of emesis associated with bacterial contamination of an infant breakfast cereal product. | a commercial product for infants containing cereal mixed with dried infant formula was diagnosed as producing rapid projectile vomiting in two infants. analysis of multiple samples of the cereal product revealed significant contamination with two spore-forming species, bacillus subtilis and a strain of bacillus cereus. the latter is the most likely cause of the emetic food poisoning, but we were unable to detect b. cereus emetic toxin. this raises the possibility of the cause being either a new ... | 2005 | 15992624 |
| antimicrobial activity of lactic acid bacteria isolated from tenerife cheese: initial characterization of plantaricin tf711, a bacteriocin-like substance produced by lactobacillus plantarum tf711. | the screening and initial characterization of bacteriocins produced by lactic acid bacteria (lab) from raw tenerife goats' cheese with possible application as biopreservatives or ripening accelerators for tenerife cheese. | 2005 | 15960667 |
| aerosolization as novel sanitizer delivery system to reduce food-borne pathogens. | as a preliminary experiment on new sanitizer delivery tools, the efficacy of aerosolized sanitizer on food-borne pathogens was investigated in larger model chamber system. | 2005 | 15960753 |
| peptidoglycan n-acetylglucosamine deacetylases from bacillus cereus, highly conserved proteins in bacillus anthracis. | the genomes of bacillus cereus and its closest relative bacillus anthracis contain 10 polysaccharide deacetylase homologues. six of these homologues have been proposed to be peptidoglycan n-acetylglucosamine deacetylases. two of these genes, namely bc1960 and bc3618, have been cloned and expressed in escherichia coli, and the recombinant enzymes have been purified to homogeneity and further characterized. both enzymes were effective in deacetylating cell wall peptidoglycan from the gram(+) bacil ... | 2005 | 15961396 |
| rare trisubstituted sesquiterpenes daucanes from the wild daucus carota. | phytochemical and biological investigation of the roots of the wild daucus carota ssp. carota afforded three new and four known compounds, including four sesquiterpenes daucane esters (1-3 [new], and 4), one polyacetylene (5), one sesquiterpene coumarin (6), and sitosterol glucoside. the structures of the new compounds were determined by comprehensive nmr studies, including dept, cosy, noesy, hmqc and hmbc analyses. based on an agar diffusion assay, 1, 2 and 4-6 were screened and found to contai ... | 2005 | 15964039 |
| in-vitro antibacterial, antifungal and cytotoxic properties of sulfonamide--derived schiff's bases and their metal complexes. | a series of new antibacterial and antifungal schiff's bases derived from sulfonamides, as well as their transition metal complexes incorporating cobalt (ii), copper (ii), nickel (ii) and zinc (ii) were synthesized, characterized and screened for their in-vitro antibacterial activity against six gram-negative (escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, salmonella typhi and shigella dysentriae) and four gram-positive (bacillus cereus, corynebacterium diphth ... | 2005 | 15968823 |
| synthesis of sphingomyelin sulfur analogue and its behavior toward sphingomyelinase. | the sulfur analogue of sphingomyelin was designed and stereoselectively synthesized from s-benzyl-n-boc-cysteine. the introduction of the phosphoryl choline moiety was successfully achieved by our own method using 2-bromoethyl dimethyl phosphite and carbon tetrabromide followed by a trimethylamine treatment. the synthesized compound proved to be a useful substrate for monitoring the enzyme activity of sphingomyelinase by detecting the liberated thiol group with a thiol-sensitive reagent. | 2005 | 15808485 |
| genomics of the bacillus cereus group of organisms. | members of the bacillus cereus group of organisms include bacillus cereus, bacillus anthracis and bacillus thuringiensis. collectively, these organisms represent microbes of high economic, medical and biodefense importance. given this significance, this group contains the highest number of closely related fully sequenced genomes, giving the unique opportunity for thorough comparative genomic analyses. much of the disease and host specificity of members of this group can be attributed to their pl ... | 2005 | 15808746 |
| molecular recognition of an adp-ribosylating clostridium botulinum c3 exoenzyme by rala gtpase. | c3 exoenzymes (members of the adp-ribosyltranferase family) are produced by clostridium botulinum (c3bot1 and -2), clostridium limosum (c3lim), bacillus cereus (c3cer), and staphylococcus aureus (c3stau1-3). these exoenzymes lack a translocation domain but are known to specifically inactivate rho gtpases in host target cells. here, we report the crystal structure of c3bot1 in complex with rala (a gtpase of the ras subfamily) and gdp at a resolution of 2.66 a. rala is not adp-ribosylated by c3 ex ... | 2005 | 15809419 |
| clinical significance of bacteriologic screening in platelet concentrates. | despite routine bacterial screening with a bacterial culturing system (bact/alert, biomerieux) of platelet (plt) concentrates, two cases of life-threatening sepsis attributed to transfused plt products contaminated with bacillus cereus were reported to the regional hemovigilance office in the southwest region of the netherlands. these reports necessitated a retrospective evaluation of the currently applied bacteriologic screening program. | 2005 | 15819671 |
| an assessment of pasteurization treatment of water, media, and milk with respect to bacillus spores. | this study evaluated the ability of spore-forming bacillus spp. to resist milk pasteurization conditions from 72 to 150 degrees c. spores from the avirulent surrogate sterne strain of bacillus anthracis, as well as a representative strain of a common milk contaminant that is also a pathogen, bacillus cereus atcc 9818, were heated at test temperatures for up to 90 min in dh2o, brain heart infusion broth, or skim milk. in skim milk, characteristic log reductions (log cfu per milliliter) for b. ant ... | 2005 | 15830666 |
| the role of sigmab in the stress response of gram-positive bacteria -- targets for food preservation and safety. | the alternative sigma factor sigmab modulates the stress response of several gram-positive bacteria, including bacillus subtilis and the food-borne human pathogens bacillus cereus, listeria monocytogenes and staphylococcus aureus. in all these bacteria, sigmab is responsible for the transcription of genes that can confer stress resistance to the vegetative cell. recent findings indicate that sigmab also plays an important role in antibiotic resistance, pathogenesis and cellular differentiation p ... | 2005 | 15831390 |
| transfer and expression of the mosquitocidal plasmid pbtoxis in bacillus cereus group strains. | the toxicity of bacillus thuringiensis subsp. israelensis to dipteran larvae (mosquitoes and black flies) depends on the presence of the pbtoxis plasmid. in this paper, two antibiotic resistance tagged pbtoxis were transferred by conjugation to other bacillus cereus group strains. among 15 potential recipients, only a lepidopteran active b. thuringiensis subspecies kurstaki and a b. cereus strain received the plasmid pbtoxis with a low transfer rate of about 10(-8) transconjugants/recipient. the ... | 2005 | 15837378 |
| plasmid-encoded regulator of extracellular proteases in bacillus anthracis. | bacillus anthracis sterne cured of the pxo1 plasmid had enhanced secreted protease activity during the postexponential phase but no change in hemolytic or lecithinase activities. a zymogen profile revealed at least six proteases, including serine, metal, and perhaps cysteine types. there were similar amounts of protease secreted by the closely related species bacillus cereus and bacillus thuringiensis, but the patterns differed. among the pxo1 plasmid-encoded proteins, there is a tetratricopepti ... | 2005 | 15838040 |
| biodegradation of imazapyr by free cells of pseudomonas fluorescene biotype ii and bacillus cereus isolated from soil. | 2005 | 15841977 | |
| production and purification of a calcium-dependent protease from bacillus cereus bg1. | the production and purification of a calcium-dependent protease by bacillus cereus bg1 were studied. the production of the protease was found to depend specifically on the calcium concentration in the culture medium. this suggests that this metal ion is essential for the induction of protease production and/or stabilisation of the enzyme after synthesis. the calcium requirement is highly specific since other metal ions (such as mg(2+) and ba(2+), which both activate the enzyme) are not able to i ... | 2005 | 15843974 |
| grafted thermo- and ph responsive co-polymers: surface-properties and bacterial adsorption. | a series of responsive polymers displaying ph and temperature-mediated phase changes were prepared from n-isopropylacrylamide and omega-carboxylic acid functionalised acrylamides. these polymers were grafted to surfaces and their characteristics probed by atomic force microscopy in aqueous solutions. the effects of ph and temperature induced phase transitions on the short-term adsorption of the bacteria salmonella typhimurium and bacillus cereus from pure cultures were assessed. contact angle st ... | 2005 | 15847993 |
| tnxo1, a germination-associated class ii transposon from bacillus anthracis. | bacillus anthracis harbours two virulence plasmids, pxo1 (182 kb) and pxo2 (95 kb). whereas pxo2 harbours the cap operon coding for the capsule, pxo1 contains the pag, lef, and cya genes coding for protective antigen, lethal, and oedema factors, respectively, as well as the atxa regulatory gene. these genes are located within a 44.8 kb long pathogenicity island flanked by insertion sequences. here, we describe the presence in the same plasmid region of an 8679 bp genetic element displaying the s ... | 2005 | 15848228 |
| use of fatty acid profiles to identify food-borne bacterial pathogens and aerobic endospore-forming bacilli. | capillary gas chromatography (gc) with flame ionization detection was used to determine the cellular fatty acid profiles of various food-borne microbial pathogens and to compare the fatty acid profiles of spores and vegetative cells of the same endospore-forming bacilli. fifteen bacteria, representing eight genera (staphylococcus, listeria, bacillus, yersinia, salmonella, shigella, escherichia, and vibrio) and 11 species were used to compare the extracted fatty acid methyl esters (fames). endosp ... | 2005 | 15853428 |
| validation of an overall model describing the effect of three environmental factors on the apparent d-value of bacillus cereus spores. | several factorial models extending the famous bigelow model to describe the influence of the heating and recovery ph and a(w) conditions on bacterial heat resistance have been developed. these models can be associated in an overall multifactorial model describing the influences of heating and recovery conditions on d values. for bacillus cereus strain adqp 407 the model parameters characterising the environmental factor influences (ph, temperature, a(w)) were evaluated. determination of bacteria ... | 2005 | 15854707 |
| [isolation and study of azobenzene converting soil bacteria]. | heterotrophic bacteria were isolated from soil and glass slides and classified as bacillus cereus snk12, paenibacillus polymyxa snk2, azotobacter chroococcum ankii, and ochrobacterium intermedium anki. their cultures could degrade azobenzene under the conditions of co-metabolism. a rapid test for the ability of bacteria to convert azobenzenes is proposed. | 2005 | 15859461 |
| the linear double-stranded dna of phage bam35 enters lysogenic host cells, but the late phage functions are suppressed. | bam35, a temperate double-stranded dna bacteriophage with a 15-kb linear genome, infects gram-positive bacillus thuringiensis cells. bam35 morphology and genome organization resemble those of prd1, a lytic phage infecting gram-negative bacteria. bam35 and prd1 have an outer protein coat surrounding a membrane that encloses the viral dna. we used electrochemical methods to investigate physiological changes of the lysogenic and nonlysogenic hosts during bam35 dna entry and host cell lysis. during ... | 2005 | 15866940 |
| use of a quartz crystal microbalance to investigate the antiadhesive potential of n-acetyl-l-cysteine. | the reduction of bacterial biofilm formation on stainless steel surfaces by n-acetyl-l-cysteine (nac) is attributed to effects on bacterial growth and polysaccharide production, as well as an increase in the wettability of steel surfaces. in this report, we show that nac-coated stainless steel and polystyrene surfaces affect both the initial adhesion of bacillus cereus and bacillus subtilis and the viscoelastic properties of the interaction between the adhered bacteria and the surface. a quartz ... | 2005 | 15870362 |
| practical application of dynamic temperature profiles to estimate the parameters of the square root model. | optimal experimental design for parameter estimation (oed/pe) is a promising method to improve parameter estimation accuracy and minimise experimental effort in the field of predictive microbiology. in this paper, the oed/pe methodology was applied on two practical examples: the growth of bacillus cereus and enterobacter cloacae in liquid whole egg product. both strains were recovered from samples of a commercial product. the goal of the modelling exercise was to quantify the influence of temper ... | 2005 | 15878409 |
| premicellar complexes of sphingomyelinase mediate enzyme exchange for the stationary phase turnover. | during the steady state reaction progress in the scooting mode with highly processive turnover, bacillus cereus sphingomyelinase (smase) remains tightly bound to sphingomyelin (sm) vesicles (yu et al., biochim. biophys. acta 1583, 121-131, 2002). in this paper, we analyze the kinetics of smase-catalyzed hydrolysis of sm dispersed in diheptanoylphosphatidyl-choline (dc7pc) micelles. results show that the resulting decrease in the turnover processivity induces the stationary phase in the reaction ... | 2005 | 15878423 |
| [heat treatment for the control of bacillus cereus spores in foods]. | the effect of heat treatments on food products related to soybean curd contaminated with bacillus cereus spores was investigated for the purpose of preventing food-poisoning outbreaks by b. cereus. in the case of b. cereus strains isolated from foods, heating foods inoculated with the spores for 20 min at 70 degrees c, 5 min at 75 degrees c, 2 min at 100 degrees c, or 10 sec with a microwave oven plus reheating after standing for 2 hr at 25 degrees c reduced the number of surviving cells within ... | 2005 | 15881248 |
| a ferric dicitrate uptake system is required for the full virulence of bacillus cereus. | bacillus cereus is an opportunistic human pathogen of increasing prevalence. analysis of the bacillus cereus genome sequence identified a potential ferric dicitrate uptake system. the three-gene operon was confirmed to be negatively regulated by the ferric uptake repressor (fur). the fec operon was genetically silenced using the integration suicide vector pmutin4. the mutant strain displayed no growth defect under iron-limited conditions but was unable to grow on ferric citrate as a sole iron so ... | 2005 | 15886918 |
| necrotizing infection due to bacillus cereus mimicking gas gangrene following penetrating trauma. | an 8-year-old boy presented with fulminant necrotizing infection resembling gas gangrene following penetrating trauma from a tree branch. bacillus cereus was isolated from tissue specimens, showing that unexpected pathogens can be isolated. it is essential to submit specimens for culture, as this organism is typically resistant to beta-lactam antibiotics and metronidazole, the empiric choice for gas gangrene. | 2005 | 15891547 |
| host-pathogen interactions: a proteomic view. | host-pathogen interactions reflect the balance of host defenses and pathogen virulence mechanisms. advances in proteomic technologies now afford opportunities to compare protein content between complex biologic systems ranging from cells to animals and clinical samples. thus, it is now possible to characterize host-pathogen interactions from a global proteomic view. most reports to date focus on cataloging protein content of pathogens and identifying virulence-associated proteins or proteomic al ... | 2005 | 15892564 |
| the exsa protein of bacillus cereus is required for assembly of coat and exosporium onto the spore surface. | the outermost layer of spores of the bacillus cereus family is a loose structure known as the exosporium. spores of a library of tn917-ltv1 transposon insertion mutants of b. cereus atcc 10876 were partitioned into hexadecane; a less hydrophobic mutant that was isolated contained an insertion in the exsa promoter region. exsa is the equivalent of safa (yrba) of bacillus subtilis, which is also implicated in spore coat assembly; the gene organizations around both are identical, and both proteins ... | 2005 | 15901704 |
| successful treatment of bacillus cereus meningitis following allogenic stem cell transplantation. | we report the case of a 19-yr-old boy, who received an allogeneic stem cell transplantation for the second relapse of hodgkin's disease. the patient developed seizures and flaccid hemiparesis on day +10. meningoencephalitis induced by bacillus cereus was diagnosed. the treatment consisted of appropriate antibiotics, g-csf and removal of the central venous line. infection control and nearly full neurological recovery was achieved. immunocompromised patients susceptible to b. cereus infection, ind ... | 2005 | 15910391 |
| bacterial strains from moldy buildings are highly potent inducers of inflammatory and cytotoxic effects. | we aimed to identify inflammatory and cytotoxic potential of individual indoor air bacterial and fungal strains, as well as extracts of indoor air filter samples containing bacteria and fungi. mouse raw264.7 macrophages were exposed in vitro to four bacterial strains; streptomyces californicus, mycobacterium terrae, bacillus cereus and pseudomonas fluorescens, and three fungal strains; penicillium spinulosum, aspergillus versicolor and stachybotrys chartarum. furthermore, raw264.7 macrophages we ... | 2005 | 15910531 |
| dynamic kinetic resolution of amino acid amide catalyzed by d-aminopeptidase and alpha-amino-epsilon-caprolactam racemase. | amino acid amide racemizing activity was discovered in alpha-amino-epsilon-caprolactam (acl) racemase (ec 5. 1. 1. 15) from achromobacter obae. the enzymatic synthesis of d-alanine from l-alanine amide has been demonstrated by use of d-aminopeptidase (dap; ec 3. 4. 11. 19) from ochrobactrum anthropi c1-38 and acl racemase. the conversion of 45 mm l-alanine amide was carried out at 30 degrees c for 7 h; l-alanine amide was completely converted to d-alanine, and no l-alanine was detected. the resu ... | 2005 | 15913357 |
| partial purification and characterization of protease enzyme from bacillus subtilis and bacillus cereus. | the aim of this experimental study was to isolate and partially purify protease enzyme from bacillus cereus and bacillus subtilis. protease enzyme is obtained by inducing spore genesis of bacteria from bacillus species in suitable nutrient plates. the partial purification was realized by applying, respectively, ammonium sulfate precipitation, dialysis, and deae-cellulose ion-exchange chromatography to the supernatant that was produced later. optimum ph, optimum temperature, ph stability, and tem ... | 2005 | 15917598 |
| microbial synthesis and characterization of physiochemical properties of polyhydroxyalkanoates (phas) produced by bacteria isolated from activated sludge obtained from the municipal wastewater works in hong kong. | the first objective of this study was the measurement of physical properties of p(3hb-co-3hv) copolymers with different (hydroxybutyrate) hb to (hydroxyvalerate) hv ratios produced by bacillus cereus (try2) isolated from activated sludge. the 3hv phbv copolymers were 0.05, 22.6, 39.2, 54.1, and 69.1 mol%, respectively. the second objective was to study possible waste water treatment and production of phas at the same time by b. cereus (try2) and pseudomonas spp. (tob17) (both were isolated from ... | 2005 | 15920276 |
| hydrocarbon biodegradation in oxygen-limited sequential batch reactors by consortium from weathered, oil-contaminated soil. | we studied the use of sequential batch reactors under oxygen limitation to improve and maintain consortium ability to biodegrade hydrocarbons. air-agitated tubular reactors (2.5 l) were operated for 20 sequential 21-day cycles. maya crude oil-paraffin mixture (13,000 mg/l) was used as the sole carbon source. the reactors were inoculated with a consortium from the rhizosphere of cyperus laxus, a native plant that grows naturally in weathered, contaminated soil. oxygen limitation was induced in th ... | 2005 | 15920621 |
| formation of the spore clumps during heat treatment increases the heat resistance of bacterial spores. | effects of the clumping of bacterial spores on their heat resistance as a result of heat treatment were investigated. spore suspensions of bacillus cereus, bacillus coagulans and bacillus licheniformis were heated at 85 degrees c. survivor curves of the three strains showed tailing in all treatments after 30 min. as the treatment time increased, the formation of spore clumps increased in all strains after 20 min. relative hydrophobicity of the spore surface increased as a result of heat treatmen ... | 2005 | 15925006 |
| chromium-resistant bacteria and cyanobacteria: impact on cr(vi) reduction potential and plant growth. | two chromium-resistant bacterial strains, bacillus cereus s-6 and ochrobactrum intermedium crt-1, and two cyanobacterial strains, oscillatoria sp. and synechocystis sp., were used in this study. at initial chromate concentrations of 300 and 600 microg k2cro4 ml(-1), and an inoculum size of 9.6 x 10(7) cells ml(-1), b. cereus s-6 completely reduced cr(vi), while o. intermedium crt-1 reduced cr(vi) by 98% and 70%, respectively after 96 h. at 100 microg k2cro4 ml(-1), synechocystis sp. mk(s) and os ... | 2005 | 15926088 |
| production of catechol-siderophore and utilization of transferrin-bound iron in bacillus cereus. | in the present study we attempted to ascertain whether bacillus cereus was able to produce catechol-siderophore(s), and whether it was able to utilize transferrin-bound iron. the growth of b. cereus was stimulated in proportion to the iron-saturation level of the transferrin, and catechol-siderophores were produced in inverse proportion to this level. b. cereus was proved to uptake iron from partially iron-saturated transferrin or holotransferrin, without destroying the transferrin by its protea ... | 2005 | 15930764 |
| characterization of chito-oligosaccharides prepared by chitosanolysis with the aid of papain and pronase, and their bactericidal action against bacillus cereus and escherichia coli. | papain (from papaya latex; ec 3.4.22.2) and pronase (from streptomyces griseus; ec 3.4.24.31) caused optimum depolymerization of chitosan at ph 3.5 and 37 degrees c, resulting in lmmc (low molecular mass chitosan) and chito-oligomeric-monomeric mixture. the yield of the latter was 14-16% and 14-19% respectively for papain- and pronase-catalysed reactions, depending on the reaction time (1-5 h). hplc revealed the presence of monomer(s) and oligomers of dp (degree of polymerization) 2-6, which was ... | 2005 | 15932346 |
| influence of glutamate on growth, sporulation, and spore properties of bacillus cereus atcc 14579 in defined medium. | a chemically defined medium in combination with an airlift fermentor system was used to study the growth and sporulation of bacillus cereus atcc 14579. the medium contained six amino acids and lactate as the main carbon sources. the amino acids were depleted during exponential growth, while lactate was metabolized mainly during stationary phase. two concentrations of glutamate were used: high (20 mm; ylhg) and low (2.5 mm; yllg). under both conditions, sporulation was complete and synchronous. s ... | 2005 | 15933027 |
| phosphatidylinositol-specific phospholipase c of bacillus anthracis down-modulates the immune response. | phosphatidylinositol-specific phospholipases (pi-plcs) are virulence factors produced by many pathogenic bacteria, including bacillus anthracis and listeria monocytogenes. bacillus pi-plc differs from listeria pi-plc in that it has strong activity for cleaving gpi-anchored proteins. treatment of murine dcs with bacillus, but not listeria, pi-plc inhibited dendritic cell (dc) activation by tlr ligands. infection of mice with listeria expressing b. anthracis pi-plc resulted in a reduced ag-specifi ... | 2005 | 15944308 |
| expanding the bactericidal action of the food color additive phloxine b to gram-negative bacteria. | phloxine b (d&c red no. 28) is a color additive for food, drugs, and cosmetics. it has been previously shown to have anti-staphylococcus aureus activities. in this work, the effect of phloxine b on various gram-negative bacteria and other gram-positive bacteria including bacillus cereus, bacillus thuringiensis, bacillus mycoides, bacillus subtilis, bacillus aureus, salmonella, escherichia coli and shigella was studied, along with the mechanism of anti-microbial activity. in the presence of fluor ... | 2005 | 15949926 |
| inhibitors of metallo-beta-lactamase generated from beta-lactam antibiotics. | the resistance of bacteria to the normally lethal action of beta-lactam antibiotics is largely due to the production of beta-lactamases that catalyze the hydrolysis of the beta-lactam. one class of these enzymes is a zinc-dependent metallo-beta-lactamase for which there are no clinically available inhibitors. the hydrolysis of cephalosporin beta-lactam antibiotics generates dihydrothiazines which subsequently undergo isomerization at c6 by c-s bond cleavage and through the intermediacy of a thio ... | 2005 | 15952764 |
| microbiological survey of prepackaged pâté and ham in new zealand. | to gauge the effectiveness of pâté and ham manufacturers' management of the microbial safety and quality of their products. | 2005 | 16033505 |
| diversity of biosurfactant producing microorganisms isolated from soils contaminated with diesel oil. | biosurfactant production is a desirable property of hydrocarbon-degrading microorganisms (hdm). we characterized biosurfactant producing microbial populations from a long beach soil, california (usa) and a hong kong soil (china), contaminated with diesel oil. a total of 33 hydrocarbon-utilizing microorganisms were isolated from the soils. twelve isolates and three defined consortia were tested for biosurfactant production and emulsification activity. the highest reduction of surface tension was ... | 2005 | 16035236 |
| insights into the genetic organization of the bacillus mycoides cryptic plasmids pdx14.2 and psin9.7 deduced from their complete nucleotide sequence. | bacillus mycoides, a member of the bacillus cereus group of bacteria, can be easily distinguished from close species because of colony shape, made by filaments of cells, resembling fungal hyphae, curving clock- or counterclockwise depending on the strain. two plasmids, one from a strain curving to the right (pdx14.2), the other from a strain curving to the left (psin9.7), were sequenced and analyzed for gene content and replication mode. rolling-circle replication modules and mobilization protei ... | 2005 | 16040120 |
| isolation of cupanioside, a novel cytotoxic and antibacterial long-chain fatty alcohol glycoside from the bark of cupania glabra. | the crude dichloromethane extract from the stem bark of cupania glabra (sapindaceae), showed in vitro cytotoxic activity against hep g2, mda-mb-231, hs 578t, mcf-7, and pc-3 cells, and antibacterial activity against bacillus cereus, staphylococcus aureus, and escherichia coli. bioactivity-directed fractionation led to isolation of the new 1-o-[2'',3'',4''-tri-o-acetyl-alpha-l-rhamnopyranosyl-(1-->2)-beta- d-glucopyranosyl]-hexadecanol (cupanioside) as the cytotoxic agent. the structure was eluci ... | 2005 | 16041658 |
| x-ray structure of the r69d phosphatidylinositol-specific phospholipase c enzyme: insight into the role of calcium and surrounding amino acids in active site geometry and catalysis. | phosphatidylinositol-specific phospholipase cs (plcs) are a family of phosphodiesterases that catalyze the cleavage of the p-o bond via transesterification using the internal hydroxyl group of the substrate as a nucleophile, generating the five-membered cyclic inositol phosphate as an intermediate or product. to better understand the role of calcium in the catalytic mechanism of plcs, we have determined the x-ray crystal structure of an engineered plc enzyme from bacillus thuringiensis to 2.1 a ... | 2005 | 16042375 |
| conjugative plasmid paw63 brings new insights into the genesis of the bacillus anthracis virulence plasmid pxo2 and of the bacillus thuringiensis plasmid pbt9727. | bacillus cereus, bacillus anthracis and bacillus thuringiensis belong to the genetically close-knit bacillus cereus sensu lato group, a family of rod-shaped gram-positive bacteria. paw63 is the first conjugative plasmid from the b. cereus group to be completely sequenced. | 2005 | 16042811 |
| occurrence and significance of bacillus cereus and bacillus thuringiensis in ready-to-eat food. | among 48,901 samples of ready-to-eat food products at the danish retail market, 0.5% had counts of bacillus cereus-like bacteria above 10(4) cfu g(-1). the high counts were most frequently found in starchy, cooked products, but also in fresh cucumbers and tomatoes. forty randomly selected strains had at least one gene or component involved in human diarrhoeal disease, while emetic toxin was related to only one b. cereus strain. a new observation was that 31 out of the 40 randomly selected b. cer ... | 2005 | 16043311 |
| enhanced photocatalytic inactivation of bacterial spores on surfaces in air. | tio(2) photocatalysis with ultraviolet (uv-a) light has proven to be a highly effective process for complete inactivation of airborne microbes. however, the overall efficiency of the technology needs to be improved to make it more attractive as a defense against bio-terrorism. the present research investigates the enhancement in the rate of destruction of bacterial spores on metal (aluminum) and fabric (polyester) substrates with metal (silver)-doped titanium dioxide and compares it to conventio ... | 2005 | 16044291 |