Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| the upstream regulatory region of human papillomavirus type 31 is insensitive to glucocorticoid induction. | the upstream regulatory region (urr) of various types of human papillomaviruses (hpvs) has been shown to contain functional glucocorticoid response elements (gres), including hpv type 11 (hpv11), hpv16, and hpv18. glucocorticoids have been demonstrated to induce the transcriptional activity of the early promoters of these hpv types. although it has been assumed that the urr of hpv31 contains at least one gre, no functionality has been demonstrated. we attempt to show here inducibility of the urr ... | 2002 | 12208949 |
| human telomerase and its regulation. | the telomere is a special functional complex at the end of linear eukaryotic chromosomes, consisting of tandem repeat dna sequences and associated proteins. it is essential for maintaining the integrity and stability of linear eukaryotic genomes. telomere length regulation and maintenance contribute to normal human cellular aging and human diseases. the synthesis of telomeres is mainly achieved by the cellular reverse transcriptase telomerase, an rna-dependent dna polymerase that adds telomeric ... | 2002 | 12208997 |
| human papillomavirus genotype prevalence in high-grade squamous intraepithelial lesions and colposcopically normal women from zimbabwe. | 2002 | 12209615 | |
| escape of p53 protein from e6-mediated degradation in hela cells after cisplatin therapy. | we previously reported that therapy of human cervical carcinoma hela cells with cp induced segregation of nucleoli and changes of nuclei characteristic of apoptosis. we raised the question of whether p53 can be reactivated by chemotherapy in hela cells despite the presence of hpv-encoded e6 activity. cellular levels of p53 protein increased after cp treatment, reaching a maximum after 6 hr. p53 protein accumulated preferentially in the nucleoli, with a peak after 15 hr. cp-induced nucleolar targ ... | 2002 | 12209989 |
| content of folic acid and free homocysteine in blood serum of human papillomavirus-infected women with cervical dysplasia. | the authors estimated the concentrations of folic acid and free homocysteine in the blood serum of women with cin iii (cervical intraepithelial neoplasia-burghard's classification) infected with dna hpv (human papillomaviruses) of type 16 and/or 18. the control group consisted of 49 patients with normal cytological smears without hpv infection. types 16 and/or 18 dna hpv were found in 50 patients. this women qualified for the studied group. the sequence of dna hpv type 16 and/or 18 was identifie ... | 2002 | 12214730 |
| validation of flt uptake as a measure of thymidine kinase-1 activity in a549 carcinoma cells. | the thymidine analog (18)f-3'-deoxy-3' -fluorothymidine (flt) is being used clinically for pet imaging of tumor proliferation. appropriate use of this tracer requires validating the mechanisms by which it accumulates in dividing cells. we tested the accuracy with which flt uptake predicted the activity of cytosolic thymidine kinase-1 (tk(1)), an enzyme that is upregulated before and during dna synthesis. | 2002 | 12215561 |
| genetic alterations in cervical carcinomas: frequent low-level amplifications of oncogenes are associated with human papillomavirus infection. | the development of cervical carcinoma is closely associated with hpv infection. however, other genetic alterations also play an important role. in this study, we analyzed copy number alterations of several oncogene loci in a panel of 84 cervical tumors. sixty-five (77%) tumors were hpv dna-positive, and most were infected with type 16 or type 18 or both. the oncogenes studied include pik3ca at 3q26.3, tert at 5p15.33, c-myc at 8q24, ccnd1 at 11q13.3, erbb2 at 17q21.2 and locus region 20q13.2. am ... | 2002 | 12216070 |
| papillomaviruses: death-defying acts in skin cancer. | ultraviolet (uv) radiation is an environmental agent that has a major impact on humans, and cumulative exposure poses a serious risk in terms of developing skin cancer. acute doses of uv induce apoptotic cell death in the skin via signalling pathways that are, in part, dependent on the p53 tumour suppressor protein. however, p53-independent mechanisms have also been described. recent findings show that a high proportion of non-melanoma skin cancers contain human papillomavirus. the viral e6 prot ... | 2002 | 12223312 |
| vitamin a, carotenoids, and risk of persistent oncogenic human papillomavirus infection. | oncogenic human papillomavirus (hpv) infection is the main etiologic factor for cervical neoplasia, although infection alone is insufficient to produce disease. cofactors such as nutritional factors may be necessary for viral progression to neoplasia. results from previous studies have suggested that higher dietary consumption and circulating levels of certain micronutrients may be protective against cervical neoplasia. this study evaluated the role of vitamin a and carotenoids on hpv persistenc ... | 2002 | 12223432 |
| differences in factors associated with oncogenic and nononcogenic human papillomavirus infection at the united states-mexico border. | previous studies have shown that risk factors for oncogenic (high-risk) and nononcogenic (low-risk) human papillomavirus (hpv) infection differ. to determine the risk factors for oncogenic and nononcogenic hpv infection in women residing along the united states-mexico border, a cross-sectional study of type-specific hpv prevalence was conducted. women (n=2246) ages 15-79 years, living in communities on both sides of the united states-mexico border and attending family planning clinics were recru ... | 2002 | 12223441 |
| detection of human papillomavirus in esophageal carcinoma. | the aim of the study was to assess the prevalence of human papillomavirus (hpv) in the esophagus in the coastal region of eastern guangdong, southern china, an area with a high incidence of esophageal carcinoma. fresh surgical resection esophageal specimens were obtained from 176 esophageal carcinoma patients admitted to the tumor hospital of shantou university medical college. the samples were subjected to polymerase chain reaction (pcr) to detect hpv infection using consensus and type-specific ... | 2002 | 12226830 |
| comparisons of hpv dna detection by my09/11 pcr methods. | two modifications to the original l1 consensus primer human papillomavirus (hpv) pcr method, my09-my011, using amplitaq dna polymerase (my-taq), were evaluated for hpv dna detection on clinical specimens from a cohort study of cervical cancer in costa rica. first, hpv dna testing of 2978 clinical specimens by my09-my011 primer set, using amplitaq gold dna polymerase (my-gold) were compared with my-taq testing. there was 86.8% total agreement (kappa = 0.72, 95%ci = 0.70-75) and 69.6% agreement am ... | 2002 | 12226831 |
| type specific persistence of high risk human papillomavirus (hpv) as indicator of high grade cervical squamous intraepithelial lesions in young women: population based prospective follow up study. | to investigate the role of human papillomavirus (hpv) in the development of cervical neoplasia in women with no previous cervical cytological abnormalities; whether the presence of virus dna predicts development of squamous intraepithelial lesion; and whether the risk of incident squamous intraepithelial lesions differs with repeated detection of the same hpv type versus repeated detection of different types. | 2002 | 12228133 |
| testing for human papillomavirus in women with abnormal pap smear results. | 2002 | 12234220 | |
| prevalence and risk factors of sexually transmitted infections and cervical neoplasia in women's health clinics in nicaragua. | to determine prevalence and risk factors of sexually transmitted infections (stis), hiv, and cervical neoplasia in women attending women's health clinics in nicaragua, and to assess the potential impact of screening for these diseases. | 2002 | 12238655 |
| risk factors for genital hpv dna in men resemble those found in women: a study of male attendees at a danish std clinic. | genital infection with certain types of human papillomavirus (hpv) is the most important risk factor for cervical cancer. the male sexual partner is supposed to be the vector of the infection. however, the knowledge of risk factors for genital hpv dna in men is limited. the objective of this paper is to study the risk factors for hpv infection in men and to compare them with those found in women, including the study of whether there are different risk profiles for oncogenic and non-oncogenic hpv ... | 2002 | 12238658 |
| transcriptional activity among high and low risk human papillomavirus e2 proteins correlates with e2 dna binding. | the full-length e2 protein, encoded by human papillomaviruses (hpvs), is a sequence-specific transcription factor found in all hpvs, including cancer-causing high risk hpv types 16 and 18 and wart-inducing low risk hpv types 6 and 11. to investigate whether e2 proteins encoded by high risk hpvs may function differentially from e2 proteins encoded by low risk hpvs and animal papillomaviruses, we conducted comparative dna-binding and transcription studies using electrophoretic mobility shift assay ... | 2002 | 12239214 |
| e7 abolishes raf-induced arrest via mislocalization of p21(cip1). | the cellular response to oncogenic ras depends upon the presence or absence of cooperating mutations. in the absence of immortalizing oncogenes or genetic lesions, activation of the ras/raf pathway results in a p21(cip1)-dependent cellular arrest. the human papillomavirus oncoprotein e7 transforms primary cells in cooperation with ras and abolishes p21(cip1)-mediated growth arrest in the presence of various antimitogenic signals. here we have utilized a conditional raf molecule to investigate th ... | 2002 | 12242284 |
| [detection of hpv16 e6 gene in cervical tissues by quantitative polymerase chain reaction]. | to study the relationship between the quantities of hpv16 e6 gene in cervical tissues and the course of cervical disease. | 2001 | 11986698 |
| primary care of hpv management in hiv-infected women. | the natural history of human papillomavirus (hpv) differs in women infected with (human immunodeficiency virus) hiv when compared with the general population. this report provides insight into the complexities of treating hpv infection and the differences found in hiv-infected women. by understanding the challenges associated with this opportunistic infection in hiv-infected women, nurse practitioners will be better prepared to provide primary care to this specific population. | 2001 | 11889689 |
| human papillomavirus, smoking, and cancer. | the effect of smoking on human papillomavirus (hpv) activity and subsequent dysplasia and neoplasia remains controversial. | 2001 | 11907844 |
| parp determines the mode of cell death in skin fibroblasts, but not keratinocytes, exposed to sulfur mustard. | sulfur mustard is cytotoxic to dermal fibroblasts as well as epidermal keratinocytes. we demonstrated that poly(adp-ribose) polymerase (parp) modulates fas-mediated apoptosis, and other groups and we have shown that parp plays a role in the modulation of other types of apoptotic and necrotic cell death. we have now utilized primary dermal fibroblasts, immortalized fibroblasts, and keratinocytes derived from parp(-/-) mice and their wildtype littermates (parp(+/+)) to determine the contribution o ... | 2001 | 11886524 |
| human papillomavirus antigens and t-cell recognition. | this review will summarize what is known about natural t-cell responses to human papillomavirus infections, including potential mechanisms for their dysregulation which may lead to the development of disease. we will also describe new strategies to enhance human papillomavirus specific t-cell responses following vaccination that are currently in development and recent reports on human vaccine trials with particular regard to the generation of appropriate t-cell responses. | 2001 | 11979123 |
| resolution of recurrent perianal condylomata acuminata by topical cidofovir in patients with hiv infection. | anogenital condylomata acuminata are the most frequent clinical manifestation of genital human papillomavirus (hpv) infection. association between human immunodeficiency virus (hiv) and hpv infections is frequent (range: 26-60% in males). topical cidofovir (a nucleotide analogue antiviral drug active against a broad range of dna viruses) is a potential treatment for anogenital warts in immunocompromised patients. we treated three hiv-infected patients with hpv perianal condylomas with topical 1% ... | 2001 | 11843219 |
| repeatability of sexual history in longitudinal studies on hpv infection and cervical neoplasia: determinants of reporting error at follow-up interviews. | misclassification of sexual history due to faulty recall or reporting bias may be the reason for variability in the association between sexual history and human papillomavirus (hpv) infection seen in studies conducted in different geographical areas. this study aimed to assess the repeatability of questionnaire information on sexual-history variables and their correlates, using information from repeat interviews by six international prospective cohort studies. | 2001 | 11822729 |
| sexual and non-sexual transmission of human papillomavirus. | benign tumors and lesions of the anogenital tract are caused by human papillomaviruses (hpvs). they are also major risk factors for cervical cancer. introduction of the polymerase chain reaction (pcr) revealed that hpv infections are much more common among young asymptomatic women than it had been previously suspected. the side-specificity of genital hpvs led to the assumption that hpvs were primarily transmitted by sexual contact. however, since hpvs have been detected in virgins, infants/child ... | 2001 | 11791348 |
| methemoglobin is a supplement for in vitro culture of human nasopharyngeal epithelial cells transformed by human papillomavirus type 16 dna. | npc-n cells were normal human nasopharvngeal epithelial cells transformed by transfection with human papillomavirus type 16 deoxyribonucleic acid. bovine pituitary extract (bpe) was one of the indispensable ingredients for in vitro culture of npc-n cells in a serum-free medium. chromatographic fractionation of bpe and subsequent immunoblotting analyses identified the hemoglobin growth-stimulating factor. methemoglobin (methb) was then synthesized, and also found to be growth stimulating. the gro ... | 2001 | 11776972 |
| rapid induction of cytotoxic t-cell response against cervical cancer cells by human papillomavirus type 16 e6 antigen gene delivery into human dendritic cells by an adeno-associated virus vector. | we have shown that the pulsing of dendritic cells (dcs) with human papillomavirus type 16 (hpv-16) antigen proteins by lipofection stimulates class i-restricted cytotoxic t lymphocyte (ctl) response against primary cervical cancer cells. also, we have shown that adeno-associated virus (aav) was able to effectively deliver a cytokine gene into dcs. it has been our hypothesis that the delivery of antigen genes into dcs, resulting in endogenous and continuous antigen protein expression, may result ... | 2001 | 11781657 |
| biological activities and molecular targets of the human papillomavirus e7 oncoprotein. | the human papillomavirus (hpv) e7 protein is one of only two viral proteins that remain expressed in hpv-associated human cancers. hpv e7 proteins share structural and functional similarities with oncoproteins encoded by other small dna tumor viruses such as adenovirus e1a and sv40 large tumor antigen. the hpv e7 protein plays an important role in the viral life cycle by subverting the tight link between cellular differentiation and proliferation in normal epithelium, thus allowing the virus to ... | 2001 | 11753671 |
| [studies on the relationship between clinicopathological features and human papillomavirus types in female lower genital tract carcinoma]. | to investigate the relationship between clinicopathological features and human papillomavirus types in female lower genital tract carcinoma. | 2001 | 11758209 |
| [human papillomavirus (hpv) in the laryngeal carcinoma]. | epidemiological data and results of molecular studies suggest that human papillomavirus has been considered a risk factor in upper respiratory tract cancers. 10 out of 23 studied cases of laryngeal carcinoma revealed presence of hpv dna (43.5%). the pcr and hybrid capture ii test were used for the detection of viral dna. | 2001 | 11765440 |
| human papillomaviruses and their role in cervical cancer. | human papillomaviruses (hpvs) have been linked to a variety of human diseases, most notably cancer of the cervix, a disease responsible for at least 200,000 deaths per year worldwide. over 100 different types of hpv have been identified and these can be divided into two groups. low-risk hpv types are the causative agent of benign warts. high-risk hpv types are associated with cancer. this review focuses on the role of high-risk hpv types in cervical tumorigenesis. recent work has uncovered new c ... | 2001 | 11766888 |
| spontaneous malignant transformation of human ovarian surface epithelial cells in vitro. | epithelial ovarian cancer has no reliable marker for early detection and no known specific premalignant changes. human ovarian surface epithelial (hose) cells expressing human papillomavirus type 16 (hpv-16) e6/e7 genes undergo crisis, and surviving cells exhibit an immortalized phenotype. cells show an increasingly invasive phenotype on collagen rafts over time. to ascertain the nature of this aberrant growth, we characterized this spontaneous progression of hose cells from a benign to an invas ... | 2001 | 11751530 |
| high frequency of loss of heterozygosity in vulval intraepithelial neoplasia (vin) is associated with invasive vulval squamous cell carcinoma (vscc). | vulval intraepithelial neoplasia (vin) is thought to be the premalignant phase of human papillomavirus (hpv)-associated vulval squamous cell carcinoma (vscc). various molecular events have been suggested as markers for progression from vin to vscc, but loss of heterozygosity (loh) in vulval neoplasia has rarely been studied in this context. we performed loh analysis by polymerase chain reaction (pcr) amplification of polymorphic microsatellite markers at 6 chromosomal loci (17p13-p53, 9p21-p16, ... | 2001 | 11745496 |
| papillary immature metaplasia of the uterine cervix: a report of 5 cases with an emphasis on the differential diagnosis from reactive squamous metaplasia, high-grade squamous intraepithelial lesion and papillary squamous cell carcinoma. | papillary immature metaplasia (pim) is a distinctive exophytic lesion of the uterine cervix and shares some histologic and cytologic features with ordinary squamous metaplasia (sm), atypical immature squamous metaplasia (aim), high-grade squamous intraepithelial neoplasia (hsil) and papillary squamous cell carcinoma (psc). pim has been suggested to be a subset of condyloma associated with low-risk type human papilloma virus (hpv), however, the etiologic role of hpv and biologic behavior of the d ... | 2001 | 11748359 |
| schneiderian papillomas and carcinomas: a review. | the ectodermally derived schneiderian mucosa gives rise to an extremely varied collection of benign and malignant neoplasms. prototypical of these are the schneiderian papillomas (inverted, fungiform, and cylindrical cell) and their malignant counterparts. human papilloma virus (hpv) is currently the leading candidate as a cofactor in the pathogenesis of the papillomas. carcinomas arise in association with the papillomas in about a 10% frequency. the carcinomas may be synchronous or metachronous ... | 2001 | 11236954 |
| cell-mediated immune response to human papillomavirus infection. | 2001 | 11238198 | |
| human papillomavirus dna detection in sperm using polymerase chain reaction. | to detect human papillomavirus (hpv) in semen and find if sperm washing removes hpv dna. | 2001 | 11239636 |
| human papillomavirus variants and squamous neoplasia of the cervix. | human papillomaviruses (hpvs) play a central role in the aetiology of cervical neoplasia. however, only a small proportion of cervical intraepithelial lesions infected with high-risk hpvs will progress to invasive cervical carcinoma, which indicates the involvement of additional factors. an important emerging viral factor is naturally occurring intratypic sequence variation. such variation has been used to study the geographical spread of hpvs, but there is increasing evidence that it may be imp ... | 2001 | 11241407 |
| detection of genetic changes in anal intraepithelial neoplasia (ain) of hiv-positive and hiv-negative men. | compared with hiv-negative individuals, hiv-positive individuals have a higher prevalence of anogenital human papillomavirus (hpv) infection, as well as a higher incidence of hpv-associated anal cancer. little is currently known of chromosomal changes occurring in anal intraepithelial neoplasia (ain), the probable precursor to anal cancer. genetic changes in ain were characterized by comparative genomic hybridization (cgh) in a study of samples obtained from 19 hiv-positive and 11 hiv-negative m ... | 2001 | 11242198 |
| human papillomavirus dna in plasma of patients with cervical cancer. | human papillomavirus (hpv) is a crucial etiological factor for cervical cancer (cc) development. from a diagnostic view-point, the consistent presence of hpv in cc allows the viral dna to be used as a genetic marker. the aims of this study were to evaluate the presence, physical status and clinical significant of hpv dna in circulation of cc patients. | 2001 | 11244579 |
| comparison of information processing technologies. | to examine the type of information obtainable from scientific papers, using three different methods for the extraction, organization, and preparation of literature reviews. | 2001 | 11230385 |
| transitional cell carcinoma of the endometrium associated with a benign ovarian brenner tumor: a case report. | transitional cell carcinomas (tccs) of the endometrium are rare, and only 10 cases have been described to date. we report the case of a 46-year-old woman who developed both a tcc of the endometrium and a benign ovarian brenner tumor. such an association has not yet been reported in the literature. immunohistochemical studies of the uterine tumor showed cytokeratin 7 positivity and cytokeratin 20 negative staining, which was consistent with a müllerian derivation. human papilloma virus (hpv) immu ... | 2001 | 11230712 |
| associations between smoking and adenocarcinomas and squamous cell carcinomas of the uterine cervix (united states). | few studies of smoking and cervical carcinoma have addressed the rare cervical adenocarcinomas or used dna-based tests to control for human papillomavirus (hpv) infection. | 2001 | 11246844 |
| management of women with minor abnormalities of the cervix detected on screening: a qualitative study. | to examine the consistency of decisions by pathology staff and general practitioners in managing women in whom minor cervical abnormalities are detected by screening, with current national health and medical research (nhmrc) council guidelines, and to look at reasons for inconsistencies. | 2001 | 11247615 |
| human papillomavirus and p53 protein immunoreactivity in condylomata acuminatum and squamous cell carcinoma of penis. | to determine the immunoreactive pattern of human papillomavirus (hpv) antigen and p53 protein in condylomata acuminatum (ca) and squamous cell carcinoma (scc) of penis. | 2001 | 11250799 |
| therapeutic potential of protein and adjuvant vaccinations on tumour growth. | over 90% of cervical cancers are associated with hpv infection, the commonest being the hpv-16 subtype. two early viral genes, e6 and 7, play major roles in the development and maintenance of the malignant phenotype. the vaccine potential of a recombinant hpv16 e7 protein was examined in two murine models of e7-expressing tumours. formulations including the immunostimulants mpl and qs21 induced therapeutically active immune responses leading to regression of pre-established tc1 tumour lesions, a ... | 2001 | 11257396 |
| human papillomavirus (hpv) dna in primary cervical cancer and in cancer free pelvic lymph nodes--correlation with clinico-pathological parameters and prognostic significance. | to assess whether the presence of human papilloma virus (hpv) dna and/or several genotypes of hpv dna in primary cervical cancer and cancer free pelvic lymph nodes are correlated with several clinicopathological parameters of well-defined prognostic significance and whether virological parameters are predictors of long-term survival in cancer patients. | 2001 | 11265139 |
| [value of estrogen and progesterone receptors in the management of intraepithelial squamous lesions of low grade]. | the objective of this study was to know the prognostic value of estrogen receptors and progesterone receptors positives (er+, pr+) in the management of low grade squamous intraepithelial lesions [(lgsil), human papillomavirus (hpv) and cervical intraepithelial neoplasia grade 1 (cin 1) and menopausal status]. | 2001 | 11268728 |
| [molecular changes in epidermoid carcinoma of the oropharynx]. | in most of the studies about molecular alterations in squamous cell carcinomas of the head and neck there is not distinction between the different subsites of this area. the objective of this study is to describe the molecular alterations in squamous cell carcinomas of the oropharynx. twenty-nine oropharyngeal carcinomas, with a minimum follow-up of 36 months, were studied. the molecular alterations analyzed were: the amplification of 11q13 region (in the 29 cases), and the myc and erbb1 oncogen ... | 2001 | 11269875 |
| physical state of hpv16 and chromosomal mapping of the integrated form in cervical carcinomas. | using a procedure based on restriction enzyme cleavage, self-ligation, and inverse polymerase chain reaction (rlipcr), the authors investigated 18 cervical intraepithelial neoplasia iii (cin iii) cases and 37 invasive squamous carcinomas for integration of human papillomavirus type 16 (hpv16). all eighteen cin iii cases (severe dysplasia or high-grade squamous intraepithelial lesion) were found to harbor episomal hpv, but one of the samples contained mixed episomal and integrated forms. seventee ... | 2001 | 11277395 |
| dual growth arrest pathways in astrocytes and astrocytic tumors in response to raf-1 activation. | normal human fibroblasts have been shown to undergo a p16(ink4a)-associated senescence-like growth arrest in response to sustained activation of the ras/raf/mek/erk pathway. we noted a similar p16(ink4a)-associated, senescence-like arrest in normal human astrocytes in response to expression of a conditional form of raf-1. while hpv16 e7-mediated functional inactivation of the p16(ink4a)/prb pathway in astrocytes blocked the p16(ink4a)-associated growth arrest in response to activation of raf-1, ... | 2001 | 11278920 |
| detection of integrated papillomavirus sequences by ligation-mediated pcr (dips-pcr) and molecular characterization in cervical cancer cells. | human papillomavirus (hpv) genomes usually persist as episomal molecules in hpv associated preneoplastic lesions whereas they are frequently integrated into the host cell genome in hpv-related cancers cells. this suggests that malignant conversion of hpv-infected epithelia is linked to recombination of cellular and viral sequences. due to technical limitations, precise sequence information on viral-cellular junctions were obtained only for few cell lines and primary lesions. in order to facilita ... | 2001 | 11279600 |
| investigation of human papillomavirus in transitional cell carcinomas of the urinary bladder in south africa. | to investigate the prevalence of human papillomavirus in transitional cell carcinoma of the urinary bladder in south africa. | 2001 | 11280602 |
| the effect of stopping smoking on cervical langerhans' cells and lymphocytes. | to investigate the effects of stopping smoking on cervical langerhans' cells and lymphocytes. | 2001 | 11281472 |
| detection of high-risk subtypes of human papillomavirus in cervical swabs: routine use of the digene hybrid capture assay and polymerase chain reaction analysis. | human papillomaviruses (hpvs) are major causative agents in the pathogenesis of cervical cancer, and more than twenty types are associated with its development. with the introduction of liquid-based preparation systems, it is envisaged that large-scale hpv testing will be established in the near future. preliminary studies demonstrate the accessibility of these samples for dna testing using both the digene hybrid capture assay (dhca) and polymerase chain reaction (pcr) techniques. this study aim ... | 2001 | 11284220 |
| significance of high-risk human papillomavirus detection by polymerase chain reaction in primary cervical cancer screening. | the purposes of this study were to evaluate the incidence of high-risk human papillomavirus (hpv) infection by polymerase chain reaction (pcr) and to assess its diagnostic usefulness in primary cervical screening. pcr testing for hpv type 16, 18, 31 and 33 was performed on 1305 specimens obtained during routine cervical cancer screening. we analysed the concurrent cervical smears and biopsy, and correlated them with the hpv infection status. we also evaluated histologically-proven cases with asc ... | 2001 | 11284951 |
| herpesvirus infection of seborrheic keratoses. | we present three examples of patients with seborrheic keratoses complicated by necrotizing herpesvirus infection. two patients had localized cutaneous herpetic infections, and the third patient had a generalized cutaneous herpesvirus infection. two of the lesions were thought to be squamous cell carcinoma. the third was clinically identified as inflamed seborrheic keratosis. herpesvirus infection was not clinically suspected in two of the patients. the histologic changes were similar in all case ... | 2001 | 11285412 |
| will new human papillomavirus diagnostics improve cervical cancer control efforts? | with the causal link between specific types of human papillomavirus (hpv) and cervical cancer firmly established, efforts have turned to assessing the relative merits of offering hpv testing in screening, triage, and posttreatment management. many unanswered questions remain, but a growing body of evidence supports a role for hpv testing in cervical cancer prevention programs. already, clinical centers that serve thousands of women in europe and the united states have incorporated hpv dna tests ... | 2001 | 11286660 |
| human papilloma virus (hpv) and p53 immunostaining in advanced tonsillar carcinoma--relation to radiotherapy response and survival. | human papilloma virus (hpv), which is frequently present in tonsillar carcinoma seems to be a prognostically favourable factor for patient survival and also for low risk of relapse. since hpv may abrogate the function of wild type p53 and hence influence radiosensitivity we attempted to analyse if hpv and p53 status in tonsillar carcinoma affected tumour response to radiotherapy (rt) and patient survival. | 2001 | 11299799 |
| a murine model for the effects of pelvic radiation and cisplatin chemotherapy on human papillomavirus vaccine efficacy. | therapeutic human papillomavirus (hpv) vaccines for cervical cancer depend on a competent immune system to be effective. however, cancer patients are often found to be immunosuppressed, which could be attributable to prior radiation, chemotherapy, or the tumor burden itself. this study investigated whether pelvic radiation or cisplatin treatment affected the efficacy of an hpv vaccine and how long these effects lasted. mice were given pelvic radiation, 2 gy/day to a total dose of 45 gy, or 5 mg/ ... | 2001 | 11300486 |
| bladder cancer in africa: update. | carcinoma of the bladder is the most prevalent cancer in egypt and in most african countries. at the national cancer institute (nci), cairo, it constitutes 30.3% of all cancers. the median age at diagnosis is 46 years, with a male preponderance of 5:1. whether in egypt or other african countries such as sudan, kenya, uganda, gold coast, and senegal, it is mostly of the squamous cell type, and arises in a background of schistosomiasis or bilharziasis. tumors are usually advanced at the time of pr ... | 2001 | 11301380 |
| cervical cancer in latin america. | cervical cancer is a common cause of morbidity and mortality in developing countries. in latin america, the incidence rates in several cities are among the highest worldwide, probably due to a high frequency of risk factors and/or a low screening coverage for cervical cancer. epidemiologic studies conducted in latin america (and some in the caribbean), that have investigated the main risk factors for the disease, as well as screening coverage by papanicolaou (pap) smear, were reviewed. the preva ... | 2001 | 11301382 |
| acquired immunodeficiency syndrome-associated cancers in sub-saharan africa. | sub-saharan africa is considered home to more than 60% of all human immunodeficiency virus (hiv) infected cases, with an estimated adult prevalence of 8.0%. it is stated that this region has contributed more than 90% of childhood deaths related to hiv infection and about 93% of childhood acquired immunodeficiency syndrome (aids)-related deaths. although no country in africa is spared of the infection, the bulk is seen in east and south africa, with the highest recorded rates of 20% to 50% in zim ... | 2001 | 11301383 |
| human papillomavirus testing and the management of women with mildly abnormal cervical smears: an observational study. | 2001 | 11302900 | |
| a high frequency of allelic loss in oral verrucous lesions may explain malignant risk. | verrucous carcinoma (vc), a variant of squamous cell carcinoma (scc), is distinct from scc in morphology and behavior. the underlying genetic changes involved in the development of vc and its precursor verrucous hyperplasia (vh) are unknown. this study determined whether chromosomal regions frequently lost during the development of scc are also lost in the vh/vc variant. twenty-five vh and 17 vc were analyzed for loss of heterozygosity (loh) at 19 loci on 7 chromosome arms using microsatellite a ... | 2001 | 11304582 |
| the association of human papillomavirus 16/18 infection with lung cancer among nonsmoking taiwanese women. | lung cancer is the leading cause of cancer death in taiwanese women since 1982. high lung cancer mortality ratio of male:female in taiwan (2:1) was observed, although less than 10% of female lung cancer patients are smokers. until now, the etiological factor remains unknown. we hypothesize that high-risk human papillomavirus (hpv) 16/18 may be associated with lung cancer development based on high prevalence of p53 negative immunostainings in female lung tumors compared with that of male lung tum ... | 2001 | 11306446 |
| the cytokines tumor necrosis factor-alpha (tnf-alpha ) and tnf-related apoptosis-inducing ligand differentially modulate proliferation and apoptotic pathways in human keratinocytes expressing the human papillomavirus-16 e7 oncoprotein. | keratinocytes are the natural target cells for infection by human papillomaviruses (hpvs), most of which cause benign epithelial hyperplasias (warts). however, a subset of papillomaviruses, the "high risk" hpvs, cause lesions that can progress to carcinomas. inflammatory mediators such as tumor necrosis factor-alpha (tnf-alpha) and tnf-related apoptosis-inducing ligand (trail) are produced by cells in response to a viral infection. to determine the effects of tnf-alpha and trail on keratinocytes ... | 2001 | 11306566 |
| clinical and prognostic significance of human papillomavirus in a chinese population of cervical cancers. | to investigate the clinical and prognostic significance of human papillomavirus (hpv) in a chinese population of cervical cancers. | 2001 | 11306910 |
| physical interaction of human papillomavirus virus-like particles with immune cells. | human papillomavirus virus-like particles (hpv vlp) and chimeric vlp are immunogens that are able to elicit potent anti-viral/tumor b and t cell responses. to investigate the immunogenicity of vlp, we determined which cells of the immune system are able to bind hpv-16 vlp. vlp were found to bind very well to human and mouse immune cells that expressed markers of antigen-presenting cells (apc) such as mhc class ii, cd80 and cd86, including dendritic cells, macrophages and b cells. mab blocking st ... | 2001 | 11312251 |
| regression of canine oral papillomas is associated with infiltration of cd4+ and cd8+ lymphocytes. | canine oral papillomavirus (copv) infection is used in vaccine development against mucosal papillomaviruses. the predictable, spontaneous regression of the papillomas makes this an attractive system for analysis of cellular immunity. immunohistochemical analysis of the timing and phenotype of immune cell infiltration revealed a marked influx of leukocytes during wart regression, including abundant cd4+ and cd8+ cells, with cd4+ cells being most numerous. comparison of these findings, and those o ... | 2001 | 11312659 |
| papillomavirus-like particles induce acute activation of dendritic cells. | the role of viral structural proteins in the initiation of adaptive immune responses is poorly understood. to address this issue, we focused on the effect of noninfectious papillomavirus-like particles (vlps) on dendritic cell (dc) activation. we found that murine bone marrow-derived dendritic cells (bmdcs) effectively bound and rapidly internalized bovine papillomavirus vlps: exposure to fully assembled vlps of bovine papillomavirus, human papillomavirus (hpv)16 or hpv18, but not to predominate ... | 2001 | 11313370 |
| improving vaccine potency through intercellular spreading and enhanced mhc class i presentation of antigen. | the potency of naked dna vaccines is limited by their inability to amplify and spread in vivo. vp22, a hsv-1 protein, has demonstrated the remarkable property of intercellular transport and may thus provide a unique approach for enhancing vaccine potency. therefore, we created a novel fusion of vp22 with a model ag, human papillomavirus type 16 e7, in a dna vaccine that generated enhanced spreading and mhc class i presentation of ag: these properties led to a dramatic increase in the number of e ... | 2001 | 11313416 |
| enhancement of suicidal dna vaccine potency by linking mycobacterium tuberculosis heat shock protein 70 to an antigen. | naked dna vaccines represent an attractive approach for generating antigen-specific immunity because of their stability and simplicity of delivery. there are particular concerns with dna vaccines however, such as potential integration into the host genome, cell transformation, and limited potency. the usage of dna-based alphaviral rna replicons (suicidal dna vectors) may alleviate the concerns of integration or transformation since suicidal dna vectors eventually cause lysis of transfected cells ... | 2001 | 11313814 |
| cervical cancer: epidemiology, prevention and the role of human papillomavirus infection. | organized screening has contributed to a decline in cervical cancer incidence and mortality over the past 50 years. however, women in developing countries are yet to profit extensively from the benefits of screening programs, and recent trends show a resurgence of the disease in developed countries. the past 2 decades have witnessed substantial progress in our understanding of the natural history of cervical cancer and in major treatment advances. human papillomavirus (hpv) infection is now reco ... | 2001 | 11314432 |
| staurosporine-induced apoptosis of hpv positive and negative human cervical cancer cells from different points in the cell cycle. | in the present study, we compare the sensitivity of caski and hela cells (hpv positive, wild-type p53) and c33a cells (hpv negative, mutated p53) to a protein kinase inhibitor, the staurosporine (st). we show that st can reversibly arrest the three cervical-derived cell lines, either in g1 or in g2/m. beyond certain st concentrations or/and over 24 h exposure, the cells underwent apoptosis. this process took place in g1 and g2/m for c33a and caski plus hela cell lines, respectively. by using an ... | 2001 | 11319606 |
| control of interferon signaling in human papillomavirus infection. | human papillomaviruses (hpv) infect mucosal and cutaneous epithelium resulting in several types of pathologies, most notably, cervical cancer. persistent infection with sexually transmitted oncogenic hpv types represents the major risk factor for the development of cervical cancer. the development of hpv-associated cervical cancer has been closely linked to the expression of the viral oncogenes e6 and e7 in the tumor cells. the major viral oncoproteins, e6 and e7, target the cellular tumor suppr ... | 2001 | 11325599 |
| hpv co-factors related to the development of cervical cancer: results from a population-based study in costa rica. | we examined factors associated with high-grade squamous intraepithelial lesions (hsil) and cervical cancer among human papillomavirus (hpv)-infected women in a prevalent case-control study conducted within a population-based cohort of 10 077 women in costa rica. we compared 146 women with hpv-positive hsil or cancer (hsil/ca) against 843 hpv-positive women without evidence of hsil/ca. subjects completed a risk factor questionnaire. we evaluated the associations between exposures and hsil/ca amon ... | 2001 | 11336474 |
| centrosome abnormalities, genomic instability and carcinogenic progression. | centrosome abnormalities are a frequent finding in various malignant tumors. since centrosomes form the poles of the mitotic spindle, these abnormalities have been implicated in chromosome missegregation and the generation of aneuploid cells which is commonly found in many human neoplasms. it is a matter of debate, however, whether centrosome alterations can drive cells into aneuploidy or simply reflect loss of genomic integrity by other mechanisms. since these two models have fundamentally diff ... | 2001 | 11342187 |
| clinicopathologic features and human papillomavirus dna prevalence of warty and squamous cell carcinoma of the penis. | squamous cell carcinoma (scc) accounts for 95% of penile malignant neoplasms. a subtype of scc, named warty carcinoma (wc), is a morphologically distinct verruciform tumor with features of human papillomavirus (hpv)-related lesions. descriptions of the behavior and histologic features of this tumor are scarce in the literature. the aim of this report is to analyze the clinicopathologic features and hpv deoxyribonucleic acid status in 60 sccs and 11 wcs. the mean patient age was 46.5 +/- 15.9 yea ... | 2001 | 11342782 |
| an evaluation of infectious diseases in cervicovaginal smears from patients with atypical cells of undetermined significance. | despite all studies of infectious disease of the female genital tract, there have been a few studies of the many different types of infectious organisms on pap smears that contain abnormal cells. the purpose of this study is to evaluate the significance of infectious organisms in women with a cytologic diagnosis of atypical squamous cells of undetermined significance (ascus). this study utilizes 398 cervicovaginal smears and concurrent cervical biopsies (test and control cases) obtained from the ... | 2001 | 11347382 |
| conversion of normal to malignant phenotype: telomere shortening, telomerase activation, and genomic instability during immortalization of human oral keratinocytes. | normal somatic cells terminate their replicative life span through a pathway leading to cellular senescence, which is triggered by activation of p53 and/or prb in response to critically shortened telomere dna. potentially neoplastic cells must first overcome the senescence checkpoint mechanisms and subsequently activate telomerase to propagate indefinitely. although telomerase activation is closely associated with cellular immortality, telomerase alone is not sufficient to warrant tumorigenicity ... | 2001 | 11349961 |
| typing of human papillomavirus by pyrosequencing. | the possibility of using a new bioluminometric dna sequencing technique, called pyrosequencing, for typing of human papillomaviruses (hpv) was investigated. a blinded pyrosequencing test was performed on an hpv test panel of 67 gp5+/gp6+ pcr-derived amplification products. the 67 clinical dna samples were sequenced up to 25 bases and sequences were searched using blast. all of the samples were correctly genotyped by pyrosequencing and the results were unequivocally in accordance with the results ... | 2001 | 11351039 |
| high prevalence of human papillomavirus infection in mexican males: comparative study of penile-urethral swabs and urine samples. | although extensive information has been gathered about the prevalence and determinants of human papillomavirus infection among women, little is known about the prevalence and natural history of this infection among males. | 2001 | 11354266 |
| listeriolysin--a useful cytolysin. | 2001 | 11357827 | |
| colposcopically directed biopsy findings in the young female. | to evaluate the colposcopic findings of cervical alterations in the young sexually active female. | 2001 | 11358705 |
| feedback regulation between orphan nuclear receptor tr2 and human papilloma virus type 16. | the human tr2 orphan receptor (tr2), initially isolated from testis and prostate cdna libraries, is a member of the steroid receptor superfamily. tr2 can regulate several target genes via binding to a consensus response element (aggtca) in direct repeat orientation (aggtcax((n))aggtca, n = 0-6). here we show that tr2 is able to induce the expression of human papilloma virus type 16 (hpv-16) genes via binding to a dr4 response element in the long control region of hpv-16. additionally, one of the ... | 2001 | 11358973 |
| adhesion of cd34+ marrow precursors to human stroma is related to alphasm actin expression by human marrow myofibroblasts. | we have studied the adhesion of human marrow cd34(+) precursors to stromal layer of nontransformed human marrow myofibroblasts (normal stroma) and to different stromal cell lines immortalized by t (pu-34) or t and t (l88/5, l87/4, l2ori-, km-102) oncogenes from simian virus 40 and e6, e7 oncogenes from human papilloma virus 16 (hs-27a, hs-23). flow cytometry and western blotting studies showed that cells from all lines were stromal myofibroblasts similar to normal stroma. using an original metho ... | 2001 | 11359676 |
| enhancement of p53-dependent gene activation by the transcriptional coactivator zac1. | a recently discovered potential tumor suppressor protein, zac1, was previously shown to promote cell cycle arrest and apoptosis, and to act as a positive or negative transcriptional cofactor for nuclear receptors. since these activities are common to zac1 and p53, we tested for a functional interaction between these two proteins by investigating possible effects of zac1 on the transcriptional activator function of p53. zac1 specifically enhanced the activity of p53-responsive promoters in cells ... | 2001 | 11360197 |
| aberrant expression of cyclin d1 is associated with poor prognosis in early stage cervical cancer of the uterus. | many investigators have studied the expression of g1 phase regulatory protein in uterine cervical cancer. however, it is unclear which step of the genetic expression participates in cyclin d1 expression and what its prognostic meaning is. the aims of this study were to evaluate the regulatory level of cyclin d1 expression and the relationship between the expression of cyclin d1 and its inhibitor, p21waf1/cip1, and to evaluate their impact on the prognosis of early stage cervical cancer. | 2001 | 11371120 |
| human papillomavirus type 16 is an important infectious factor in the high incidence of esophageal cancer in anyang area of china. | to investigate the potential role of human papillomavirus (hpv) infection in the pathogenesis of esophageal carcinomas in the anyang area of china, we have evaluated specimens collected by balloon cytology examination from volunteers in two regions with significantly different incidences of esophageal carcinoma. 138 donors were from a village in a county with an esophageal carcinoma (ec) age-adjusted mortality rate of 132x10(5), the remaining 68 were resident in a second village from another cou ... | 2001 | 11375901 |
| pathogenesis of esophageal squamous cell carcinoma with lymphoid stroma. | lymphocyte infiltration in esophageal cancer, especially when beneath intraepithelial carcinoma, is frequently seen. however, cases of esophageal cancer with a dense stromal infiltration of lymphocytes are rare and the pathogenesis of such cases has yet to be clearly demonstrated. the objective of this study is to clarify its pathogenesis. | 2001 | 11379332 |
| histologic and immunophenotypic classification of cervical carcinomas by expression of the p53 homologue p63: a study of 250 cases. | recent studies of the p53 homologue p63 indicate that this gene is preferentially expressed in basal and immature cervical squamous epithelium. this study correlated p63 expression with morphologic phenotype and human papillomavirus (hpv) type in a wide range of cervical neoplasms. two hundred fifty cases of cervical carcinoma, including squamous cell carcinoma (scca; n = 178), adenocarcinoma (adca; n = 28), adenosquamous carcinoma (asca; n = 8), neuroendocrine carcinoma (neca; n = 15), and othe ... | 2001 | 11381365 |
| women's knowledge of pap smears, risk factors for cervical cancer, and cervical cancer. | to review the literature on women's knowledge of pap smears, risk factors for cervical cancer, and cervical cancer. | 2001 | 11383953 |
| [type i and iv collagenases and their endogenous regulators in immortalized and transformed fibroblasts]. | to elucidate the role of matrix metalloproteinases (mmp) in carcinogenesis, the expression of collagenases of types i (mmp-i) and iv (mmp-2 and mmp-9) as well as the behaviour of urokinase-like plasminogen activator (upa) and of tissue mmp inhibitors (timp) in immortalized (if) and transformed (tf) fibroblasts were investigated. the study was carried out using embryo rat fibroblasts, sequentially immortalized with the lt gene of human papilloma virus and transformed with the e7 gene of human pap ... | 2001 | 11386000 |
| much higher risk of premalignant and malignant cervical diseases in younger women positive for hpv16 than in older women positive for hpv16. | human papillomavirus (hpv), particularly hpv16, is strongly associated with premalignant lesions of the uterine cervix and cervical cancer. however, hpv infection is a common sexually transmitted disease and only a few women develop cervical cancer. although the presence of hpv and abnormal cytology are independent risk factors for cervical diseases, implementing both tests on every woman is argued not to be recommended mainly in terms of cost-effectiveness. during a 20-month period between octo ... | 2001 | 11386424 |
| cost-effectiveness of monolayers and human papillomavirus testing compared to that of conventional papanicolaou smears for cervical cancer screening: protocol of the study of the french society of clinical cytology. | the french society of clinical cytology is conducting a study to compare the cost-effectiveness of monolayers and human papillomavirus (hpv) testing with that of conventional papanicolaou (pap) smears for cervical cancer screening. the protocol of this study is presented. it includes 3,000 women who will be evaluated by the three methods (conventional pap smears, or monolayers with or without hpv testing) and by the reference method: colposcopy followed, in cases with abnormalities, by cervical ... | 2001 | 11391824 |
| prevalence of human papillomavirus infection among prostitutes in calcutta. | we investigated the presence of human papilloma virus (hpv) 16/18 and herpes simplex virus-1,-2 (hsv-1,-2) infections in buccal mucosal cells of prostitutes of calcutta, india, by in situ dna hybridization and immunocytochemical technique. in some of them, we also examined the prevalence of viral infections in uterine cervical cells. the women were also tested for venereal disease research laboratory antigen (vdrl) positivity. oral infections with hsv-1, -2 and hpv 16/18 were detected in 24.6%, ... | 2001 | 11394709 |
| acceptability of a human papillomavirus (hpv) trial vaccine among mothers of adolescents in cuernavaca, mexico. | a scenario that must be considered when testing prophylactic human papillomavirus (hpv) vaccines in teenagers is the parents' acceptability of their daughters' participation in the study. | 2001 | 11395192 |
| pre-clinical safety and efficacy of ta-cin, a recombinant hpv16 l2e6e7 fusion protein vaccine, in homologous and heterologous prime-boost regimens. | human papillomavirus (hpv) e6 and e7 oncoproteins are attractive targets for t-cell-based immunotherapy of cervical intraepithelial neoplasia (cin) and cancer. a newly designed vaccine, comprising the hpv16 l2, e6 and e7 as a single fusion protein (ta-cin), was shown to elicit hpv16-specific ctl, t-helper cells and antibodies in a pre-clinical mouse model. these immune responses effectively prevented outgrowth of hpv16-positive tumour cells in a prophylactic setting as well as in a minimal resid ... | 2001 | 11395199 |