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effect of mineral oil on porcine urothelium.mineral oil has been used to facilitate ureteral stone extraction and to treat selected patients with infected residual urine. the purpose of this study was to evaluate the effect of mineral oil on the urothelium. twelve adult female farm pigs underwent bilateral ureteral catheter placement under general endotracheal anesthesia. retrograde pyelograms were performed and the ureteral diameters measured. using a randomization protocol, six animals underwent injection of 10 ml of normal saline into ...19979181446
clarifying plasma melatonin profiles in domestic pigs: a critical and comparative evaluation of two radioimmunoassay systems.the results of a direct radioimmunoassay (ria) for porcine plasma melatonin, suggesting a relationship among plasma melatonin, feed intake and photoperiod, were investigated by comparison with the results of an extracted ria. these findings were further examined by analysis of a small number of samples using gas chromatography-mass spectrometry (gcms). the results identified inadequacies in the specificity of the direct ria and failed to provide evidence for an effect of feed intake on melatonin ...19979181517
fibre type grouping in porcine masseter and soleus muscles assessed by the enclosed fibre type concept. a statistical and computational analysis.muscle function is determined not only by the frequency of slow (type i) and fast (type ii) fibres but also the spatial fibre type organization. during ageing as well as in muscle disorders, divergencies from the normal pattern may occur and are expressed as 'fibre-type grouping'. to elucidate whether intramuscular differences in spatial fibre type arrangements may underlie the functional heterogeneity of the porcine masseter, the arrangements of intrafascicular type i and type ii fibres were as ...19979183034
the endothelin receptor antagonist, bosentan, in combination with the cyclooxygenase inhibitor, diclofenac, counteracts pulmonary hypertension in porcine endotoxin shock.to prevent endotoxin-induced pulmonary hypertension in the pig with a combination of a nonpeptide mixed endothelin receptor antagonist, bosentan, and a cyclooxygenase inhibitor, diclofenac.19979187606
exposure of swine to trichinella spiralis antigen as determined by consecutive elisas and western blot.exposure of swine to trichinella spiralis was evaluated using a combination of 3 consecutive enzyme-linked immunosorbent assays (elisas) based on larval t. spiralis excretory-secretory antigen as screening test and western blot analysis as confirmatory test. ninety-three of 32,693 domestic swine sera collected in georgia over a 5-yr period contained antibodies specific to t. spiralis (prevalence of exposure = 0.28%). the highest prevalence (0.52%) of exposure to t. spiralis was in samples from s ...19979194822
effect of ng-nitro-l-arginine-methyl-ester on cardiopulmonary function and biosynthesis of cyclooxygenase products during porcine endotoxemia.to determine if inhibition of nitric oxide synthase with ng-nitro-l-arginine-methyl-ester (l-name) potentiates endotoxin-induced cardiopulmonary dysfunction and release of cyclooxygenase products in a porcine model of endotoxemia.19979201060
experimental vesicular stomatitis in swine: effects of route of inoculation and steroid treatment.an enzootic focus of vesicular stomatitis virus new jersey serotype (vsv-nj) exists on ossabaw island, georgia. many questions regarding the epizootiology of this virus at this focus still exist, but evidence suggests that the vector for this virus is a phlebotomine sand fly (lutzomyia shannoni), with feral swine serving as a potential source of virus for the sand fly and for other swine via contact transmission. we conducted 2 experimental trials in domestic swine using vsv-nj isolated from a s ...19979211231
infiltrator angioplasty balloon catheter: a device for combined angioplasty and intramural site-specific treatment.we describe a new angioplasty device (infiltrator angioplasty balloon catheter; iabc) with intramural drug delivery capability. the conventional balloon part of the device, when inflated, dilates the vessel or has three rows of longitudinally mounted infiltrator nipples to penetrate the tunica media. through an independent infiltrator port and nipples, drugs can be infiltrated directly into the vessel wall.19979213033
laparoscopically guided bipolar radiofrequency ablation of areas of porcine liver.bipolar radiofrequency ablation (brfa) is a promising technique with which to treat unresectable primary and metastatic liver tumors. its effects on normal liver tissue and postoperative liver function, however, are unknown. we performed this study to determine (1) the feasibility of using laparoscopic ultrasound to guide placement of brfa needle electrodes in the liver and (2) the histopathologic, hepatic biochemical, and systemic hemodynamic responses to brfa.19979214320
mechanisms of transmission of aujeszky's disease virus originating from feral swine in the usa.to understand the possible mechanisms of transmission of aujeszky's disease virus (pseudorabies or prv) from a feral pig reservoir, intranasal infections were initiated in domestic pigs and in pigs from a herd derived from captured feral pigs. virus strains originating from feral pigs and from domestic pigs were compared. similar shedding patterns were obtained in both feral-derived and domestic pigs, however, virus strains from feral pigs were markedly attenuated. virus could be isolated after ...19979220604
catalytic activity of phospholipase a2 in serum in experimental fat embolism in pigs.to investigate the catalytic activity of phospholipase a2 in serum during the early phase of experimental fat embolism.19979231857
inotropic treatment and intestinal mucosal tissue oxygenation in a model of porcine endotoxemia.to evaluate the dose-related effects of dopamine, dopexamine, and dobutamine on intestinal mucosal tissue oxygenation following short-time infusion of escherichia coli lipopolysaccharide, which has previously been shown to decrease mucosal tissue oxygenation by 60% of control values.19979233747
experimental fat embolism induces urine 2,3-dinor-6-ketoprostaglandin f1alpha and 11-dehydrothromboxane b2 excretion in pigs.to evaluate the in vivo production of prostacyclin and thromboxane a2 during the initial phase of experimental fat embolism as assessed, respectively, by determinations of urine 2,3-dinor-6-ketoprostaglandin f1alpha and 11-dehydrothromboxane b2 excretion.19979233750
zinc-bis-histidinate preserves cardiac function in a porcine model of cardioplegic arrest.we examined the ability of zinc-bis-histidinate to preserve postarrest myocardial function when added to a standard crystalloid cardioplegic solution.19979236338
thoracoscopic transmyocardial laser revascularization.transmyocardial laser revascularization is a promising surgical technique used to treat nonreconstructable ischemic heart disease. recent clinical data show that this technique improves the regional perfusion of ischemic myocardium and reduces angina. presently, transmyocardial laser revascularization requires an open, lateral thoracotomy. we report here the use of thoracoscopic techniques to perform transmyocardial laser revascularization in a closed chest fashion.19979236355
assessment of the nutritional effects of olestra, a nonabsorbed fat replacement: introduction and overview.olestra is a mixture of polyesters formed from sucrose and fatty acids derived from edible fats and oils. it is not absorbed or digested and can serve as a zero-calorie replacement for dietary fat. because olestra is lipophilic and not absorbed, it has the potential to interfere with the absorption of other dietary components, especially lipophilic ones, when it is in the digestive tract with those components. a series of studies were conducted in the domestic pig and in healthy adult humans to ...19979237952
the domestic pig as a model for evaluating olestra's nutritional effects.experimental conditions for measuring the effect of the noncaloric fat substitute olestra on the availability of dietary nutrients were established in the weanling domestic pig. to evaluate the tolerance of the pig for dietary fat levels similar to those in the human diet, groups were fed a standard corn-soy-based swine feed with and without 14% (30% of energy) added fat for 4 wk. to evaluate the adequacy of a purified diet to produce good growth, groups of pigs were fed purified diets providing ...19979237954
physical or temporal separation of olestra and vitamins a, e and d intake decreases the effect of olestra on the status of the vitamins in the pig.a study was conducted in the domestic pig to determine 1 ) whether feeding olestra mixed in the diet exaggerated olestra effects on fat-soluble vitamin status compared with the effects of feeding it in a typical snack food, and 2) whether separating olestra consumption temporally from vitamin consumption affected the influence of olestra on vitamin status. groups of 10 pigs each, five castrated males and five females, were fed 2.2% (wt/wt) olestra for 4 wk in purified diet that provided 1 time t ...19979237955
nutritional status of pigs fed olestra with and without increased dietary levels of vitamins a and e in long-term studies.in a 26-wk study, five groups (n = 10) of domestic pigs were fed 0.25, 0.5, 1.1, 3.3 or 5.5% olestra; three groups were fed 0.25% with graded levels of vitamins a and e; and one group was fed 5.5% with added vitamins a and e and exposed to uv light. in a 39-wk study, two groups (n = 10) were fed 0.25% olestra with or without added vitamins a and e. in each study, a control group was fed basal diet with no olestra, and a group was killed at d 0 for base-line nutrient measurements. the diets provi ...19979237959
incorporation of cultured embryonic cells into transgenic and chimeric, porcine fetuses.the derivation of murine, embryonic stem cells, and their use in the generation of transgenic mice, are well-established procedures. application of these methodologies to non-murine species, however, remains to be fully realized. non-murine embryonic stem cells would be of considerable value to studies in comparative development, and would raise important implications for agriculture and biotechnology. here we report the achievement of chimerism and transgenesis in the domestic pig (sus scrofa) ...19979240573
the cytogenetic map of the domestic pig. 19979250869
continuous monitoring of the partial pressure of oxygen in cerebral venous blood.clinical oxygen monitoring in the injured brain is somewhat difficult. however, ischemia is one of the major factors responsible for secondary tissue damage after head injury or subarachnoid hemorrhage. therefore, the aim of the present study was to investigate the value of continuously monitoring the partial pressure of oxygen in cerebral venous blood (pcvo2) during changes in intracranial pressure (icp).19979257315
effects of the at1-selective angiotensin ii antagonist, telmisartan, on hemodynamics and ventricular function after cardiopulmonary resuscitation in pigs.the purpose of this study was to investigate the effects of the angiotensin ii (ang ii) antagonist, telmisartan, on hemodynamics, myocardial function and myocardial blood flow during the postresuscitation phase in a porcine model of cpr and to compare these to saline. after 4 min of ventricular fibrillation and 5 min of closed-chest cpr, defibrillation was performed in 16 domestic pigs to restore spontaneous circulation (rosc). ten minutes after rosc, animals were allocated to receive either the ...19979259062
demonstration of nerve fibers containing the c-terminal flanking peptide of neuropeptide y (cpon) in the pig pineal gland (sus domesticus): an immunocytochemical study by light and electron microscopy.the pineal gland is richly innervated by the neuropeptide y (npy)-positive nerves that mostly exibit immunoreactivity for the enzyme tyrosine hydroxylase, a marker of sympathetic nerve fibers. npy is synthesized as a part of larger prepromolecule. the present study was undertaken to demonstrate that the posttranslational processing of prepronpy resulted in the presence of c-terminal flanking peptide of npy (cpon) in nerve fibers of the pig pineal gland.19979268146
a conserved african swine fever virus ikappab homolog, 5el, is nonessential for growth in vitro and virulence in domestic swine.an african swine fever virus (asfv) gene with similarity to the cellular inhibitor of nfkappab (ikappab) was described in the pathogenic african isolate malawi lil-20/1 (orf 5el) and a cell-culture-adapted european virus, ba71v (orf a238l). recently, this gene was shown to be a functional ikappab homolog capable of downregulating nfkappab-regulated gene expression. this observation suggests the gene may be of significance to aspects of asfv pathogenesis and virulence in domestic swine by interfe ...19979281518
localization of the 18s, 5.8s and 28s rrna genes and the 5s rrna genes in the babirusa and the white-lipped peccary.the locations of the genes encoding 18s, 5.8s and 28s rrna and 5s rrna were studied in two relatives of the domestic pig, the babirusa (babyrousa babyrussa) and the white-lipped peccary (tayassu pecari). in the babirusa, the 18s, 5.8s and 28s rdna is located on chromosomes 6, 8 and 10. the genes on chromosomes 8 and 10 are actively transcribed, in contrast to those on chromosomes 6. in the white-lipped peccary, this rdna was found to be located on chromosomes 4 and 8. the genes on both of these ...19979284936
[endemic area of paragonimiasis in vietnam].we studied 155 patients with chronic pulmonary disease from a mountainous area in north-west vietnam, in march, 1993. sputum was collected from patients and examined for the presence of paragonimus eggs using a light microscope. forty four of the patients (approximately 28%) were diagnosed with and treated for human paragonimiasis. forty two of these cases were purely pleural-pulmonary forms whereas the remaining two cases also involved problems with the central nervous system. most of the patie ...19979296804
effects of one-lung ventilation on pulmonary hemodynamics and gas exchange in the newborn.for major thoracoscopic surgery, one-lung ventilation is mandatory. this is reasonably well tolerated in adults and children. the effects of one-lung ventilation on hemodynamics and gas exchange in newborns, however, are not known yet. eight neonatal domestic pigs with a median age of 6 days (range: 3-9 days), and a mean body weight of 2.3 kg (1.7-2.8 kg) were intubated and ventilated in pressure-controlled mode (fio2 = 1). anesthesia was maintained with i.v. fentanyl/metomidate. after tracheoto ...19979297515
changes in swine macrophage phenotype after infection with african swine fever virus: cytokine production and responsiveness to interferon-gamma and lipopolysaccharide.cytokines produced by cells of the immune system, including macrophages, can influence inflammatory responses to viral infection. this has been exploited by viruses, which have developed strategies to direct the immune response towards ineffective responses. african swine fever virus (asfv) is a double-stranded dna virus that infects macrophages of domestic swine. in this study, primary cells of monocyte macrophage lineage were obtained from the lungs, peritoneum or blood of domestic swine and, ...19979301535
[the epidemiological-epizootiological subdivision of the nosogeographic range of trichinosis].the prevalence of trichinosis was analyzed among human beings and animals in moscow province over 50 years (1946-1996). a total of 7022 domestic, synanthropic, and wild carnivorous animals were incompletely helminthologically dissected. enzyme immunoassay revealed diagnostic trichinella antigen antibody titers in 45.4% of 544 examinees during outbreaks and at their intervals, as well as in 0.58% of domestic pigs of different property forms. trichinella larvae were found in domestic pigs (0.13%), ...19979304022
identification and differentiation of bear bile used in medicinal products in taiwan.one hundred eighty-three suspect bear bile used in medicinal products, collected in taiwan as gall bladders or dried powder forms, were analyzed using ftir, hptlc, and hplc techniques to identify whether they are indeed bear bile. those confirmed were further examined to determine whether the observed analytical parameters can be reliably used for source inference, i.e., differentiating products among north american black bear, farmed asiatic black bear, polar bear, etc. our data suggested that ...19979304828
cloning, structural analysis, and expression of the pig ige epsilon chain.as a step in the evolutionary studies of immunoglobulin e (ige) and for the purpose of developing new reagents that will facilitate a more detailed analysis of ige-mediated inflammatory reactions in a large animal model, we here present the cloning of the epsilon chain of ige in the domestic pig (sus scrufa). a partial cdna clone for the epsilon chain of pig ige was isolated by polymerase chain reaction (pcr) amplification using degenerate primers directed against conserved regions in the second ...19979321425
likelihood of introducing selected exotic diseases to domestic swine in the continental united states of america through uncooked swill.to help policy makers determine the need for current regulations (which require cooking of swill prior to feeding to swine), an assessment of the likelihood of exposing domestic swine in the continental united states of america (usa) to selected foreign animal disease agents by feeding uncooked swill was carried out. the hazard was assumed to originate from contraband food items entering the usa and subsequently being discarded in household waste. such food waste may be collected by licensed was ...19979329117
effects of castration on early postnatal development of male accessory sex glands in the domestic pig.in the neonatal pig there is a remarkable production of steroids by the testes for the first few weeks after birth. several androgens and estrogens reach a peak at about one month of age. in order to gain an understanding of the significance of this early steroid secretion we examined the effect on accessory sex glands of removal of the testes before the peak in these compounds would have occurred. pigs were castrated (n = 38) at 2-3 weeks of age, with littermates serving as intact controls (n = ...19979330594
magnetic resonance-guided laparoscopic interstitial laser therapy of the liver.there is a necessity for an imaging method during laparoscopy to get a three-dimensional access to the target. in this study we evaluated laparoscopic interstitial laser therapy of the liver under magnetic resonance imaging guidance.19979337173
local delivery of ethanol inhibits intimal hyperplasia in pig coronary arteries after balloon injury.smooth muscle cell (smc) hyperplasia is an important mechanism of restenosis after coronary angioplasty and the primary mechanism of restenosis within coronary stents. ethanol has been shown to reduce the response of smcs to local growth stimulants in vitro. this study was carried out to determine whether local delivery of ethanol solution could reduce intimal hyperplasia induced by balloon injury.19979337203
horseradish peroxidase as a histological indicator of mechanisms of porcine retinal vascular damage and protection with perfluorocarbons after massive air embolism.this laboratory has previously shown that a second-generation perfluorocarbon emulsion (pfe) reduces the severity of cerebral injury induced by air embolism during cardiopulmonary bypass (cpb). horseradish peroxidase examines vascular permeability and was used in this study of the mechanisms of cellular protection afforded by the pfe.19979341714
status of trichinella spiralis in domestic swine and wild boar in canada.evidence of the status of trichinellosis in canada's national swine herd is provided from data acquired through national surveillance programs and from a prevalence study of trichinella in wild boar and domestic swine. more than 500,000 swine tested at abattoirs in ongoing animal health surveys since 1980 and 2 national swine serological surveys (1985 and 1990) showed no evidence of trichinella infection, except for 3 occurrences in a small infected zone in nova scotia. the prevalence study of d ...19979342448
acute effects of 17 beta-estradiol on the coronary microcirculation: observations in sedated, closed-chest domestic swine.to test the hypotheses: that acute administration of 17 beta-estradiol dilates normal coronary microvessels in vivo; that coronary microvascular responses to acute estrogen stimulation exhibit sexual dimorphism; and that nitric oxide has a role in mediating these effects.19979347215
heparin versus citrate regional anticoagulation during autotransfusion in a porcine intra-abdominal hemorrhage model.our objective was to determine the effects of anticoagulants and blood loss on hemodynamic, hematologic, and coagulation parameters following autotransfusion in an animal model of intraabdominal hemorrhage. we performed a prospective, randomized observational animal study at an animal research laboratory at a university medical center. eight landrace, domestic pigs, weighing 17-23 kg, each underwent jugular venous and iliac arterial catheterization and laparotomy with retroperitoneal dissection ...19979358794
[localization of pth gene on pig chromosome 14q25-q28 by in situ hybridization].the human parathyroid hormone (pth) genic dna, as a probe, was labelled with tritium(3h) using nick translation and random oligonucleotide primers methods. metaphase chromosomal g-bands were prepared from cultured blood lymphocytes of domestic pig. after in situ hybridization and autoradiographly, the number of silver grains on every chromosome was calculated. the result showed that pth gene is located at chromosome 14q25-q28. the relevent points of this study are discussed.19979361454
the effect of alpha-trinositol on cholera toxin-induced fluid accumulation in pig (sus scrofa domesticus) jejunum in vivo.the effect of alpha-trinositol (d-myo-inositol-1,2,6-trisphosphate) on cholera toxin-induced fluid accumulation (i.e., net fluid secretion) was studied in the pig jejunum in vivo. cholera toxin caused a dose-dependent fluid accumulation in control experiments. intravenous injection of alpha-trinositol produced a reduction of the response to cholera toxin with a significant maximal inhibition of 36%. however, in high concentrations of alpha-trinositol this inhibition was absent.19979366061
laparoscopic modified sugiura procedure: experimental study on the pig.the sugiura procedure is an alternative treatment for bleeding gastroesophageal varices. laparoscopic sugiura procedure has not previously been described. the aim of this study is to develop a laparoscopic oesophageal transection with an eea stapler, as well as a complete laparoscopic modified sugiura procedure.19979372382
the use of a wild pig x domestic pig intercross to map phenotypic trait loci.intercrosses between divergent lines of domestic animals adapted to different environmental conditions and/or production systems provide unique opportunities for mapping phenotypic trait loci. the strategy resembles the use of interspecific crosses developed for general linkage mapping. we have made an intercross between the wild pig and domestic pigs of the large white breed. a comprehensive linkage map comprising more than 230 genetic markers has been established. the successful use of this in ...19979378913
natural host odours as possible attractants for glossina tachinoides and g. longipalpis (diptera: glossinidae).as strictly haematophagous insects, tsetse flies feed on a wide variety of wild and domestic animals. although these are mainly mammals, some tsetse species also feed on reptiles. the present study investigated whether the odours of several potential natural tsetse hosts may be used as novel attractants to improve the catch of glossina tachinoides or g. longipalpis in biconical traps. the odour of a living monitor lizard (varanus niloticus) had no effect on the catch of g. tachinoides. hexane sk ...19979386790
gnathostomiasis in frog-eating snakes from japan.gnathostoma doloresi parasitizes the gastric wall of wild (boars) and domestic (pigs) swine (sus scrofa). its larvae cause cutaneous larva migrans in humans. amphibians, reptiles and a freshwater fish are infected with the advanced 3rd stage larvae. prevalence of g. doloresi larvae were surveyed in several snakes, especially in a common frog-eating snake (rhabdophis tigrinus). all species of snakes examined were infected with g. doloresi larvae suggesting that snakes are important reservoir host ...19979391975
vitamins c and e inhibit o2- production in the pig coronary artery.we previously found in a pig coronary balloon injury model that vitamins c and e as well as probucol had beneficial effects on the vessel response to injury measured by morphometry these effects correlated with an inhibition in the ability to oxidize ldls ex vivo, suggesting that the morphological response was due to the antioxidant effect of the treatments.19979396460
physiological concentrations of estradiol attenuate endothelin 1-induced coronary vasoconstriction in vivo.estrogens are cardioprotective hormones and are reported to have antianginal properties. we examined the effect of physiological concentrations of 17beta-estradiol on coronary reactivity in anesthetized female farm pigs.19979396464
diagnosis of trichinellosis in swine by enzyme immunoassay, using a synthetic glycan antigen.to assess performance of a synthetic carbohydrate antigen for use in an enzyme immunoassay for diagnosis of trichinellosis in swine. animals and sample population: 47 york x duroc pigs and field sera from 265 farm pigs raised under various management systems.19979401691
modifications of operant thermoregulatory behavior of the young pig by environmental temperature and food availability.piglets (sus scrofa domesticus) were weaned at 14 days and acclimated to 10 ("cold animals") or 35 degrees c ("warm animals"). cold animals were either fed ad lib. (10hh) or maintained on a high (10h) or low (10l) nutrition plane. warm animals were maintained on a high (35h), low (35l), or a very low (35ll) nutrition plane. after 3 weeks of acclimation, operant thermoregulatory behavior (animals pressed a lever to turn on a heater) at 15 degrees c was assessed immediately following or 22 h after ...19979402624
epidemiological investigation of trichinellosis in switzerland.domestic pigs in switzerland have been considered trichinella-free for decades, despite the occurrence of trichinella in the wildlife cycle. in order to reevaluate the present epidemiological situation, tissue samples from 11226 domestic pigs, 356 wild boars and 452 foxes were examined using the standard artificial digestion method. a simultaneous serological study, extended to include 25239 sera from sows provided by a swiss pig serum bank, was also undertaken. the results of both studies suppo ...19979404846
vascular endothelial growth factor/vascular permeability factor produces nitric oxide-dependent hypotension. evidence for a maintenance role in quiescent adult endothelium.in vitro studies suggest that vascular endothelial growth factor/vascular permeability factor (vegf/vpf) may stimulate release of nitric oxide (no) from endothelial cells. to investigate the hemodynamic consequences of recombinant vegf/vpf administered in vivo, recombinant human vegf/vpf was administered as a bolus dose of 500 micrograms to anesthetized (n = 6) or conscious (n = 5) new zealand white rabbits, as well as anesthetized rabbits with diet-induced hypercholesterolemia (hc; n = 7). anes ...19979409257
[cava filter placement under mri control. experimental in vitro and in vivo studies].an instrument has been developed for the introduction of caval filters which can be used with mri; it has been investigated in in vitro and in in vivo experiments.19979417273
functional mr imaging of the porcine kidney: physiologic changes of prolonged pneumoperitoneum.increased intraabdominal pressure (ipa) during laparoscopy has been associated with decreased urine output. the purpose of this study was to use a noninvasive mri technique to measure renal vessel flow velocity and change in differential renal medulla and cortex perfusion during pneumoperitoneum.19979876643
epidemiology of echinococcus multilocularis and e. granulosus in central europe.two species of echinococcus are known to occur in central europe, namely e. multilocularis and echinococcus granulosus, causing the alveolar and the cystic form of echinococcosis in humans, respectively. recent studies have shown that in central europe e. multilocularis occurs further north, south and east than previously anticipated. this parasite is endemic in belgium, luxembourg, france, switzerland, liechtenstein, austria, germany, poland, and the czech republic. the prevalence rates of e. m ...19979802089
pigs in space: spatial memory and its susceptibility to interference.this study provided basic information about spatial memory in the domestic pig, sus scrofa and examined how susceptible it is to disruption by environmental stimuli. eight male pigs were tested individually in a foraging arena. each day, they entered the arena to search for food randomly located in one of 10 enclosed areas (search trial). after finding and eating the food, they were removed from the arena for a retention interval, and then allowed back in to relocate the food in the same area as ...19979794774
[the function of nitric oxide in the necrosis of avulsed skin flap in domestic pig].to investigate the function of nitric oxide (no) and nitric oxide synthetase (nos) inhibitor, n-nitro-l-arginine methyl ester (l-name), the skin avulsion model was made in the lower extremity of pig. the methods of measurement of size of the survived flap, weighing, immunocytochemistry and hybridization in situ were employed, so that the survival surface area of flaps, tissue wet/dry weight ratio, no content in the serum, gene expression of no and nos content in the flap tissue were determined, ...19979867957
expression pattern of extracellular matrix proteins in the pancreas of various domestic pig breeds, the goettingen minipig and the wild boar.in spite of progress in biotechnology, isolation of porcine pancreatic islets remains a difficult task with unpredictable results. one reason could be the lack of knowledge as to the expression of extracellular matrix proteins in porcine exocrine and endocrine tissues, particularly in "islet capsules". such proteins are subject to digestion by proteases, yet they might have a protective function for the fragile islets.19979869860
endotracheal drug delivery followed by a 90-degree torso tilt in a swine model of cardiac arrest.prehospital providers are often unable to obtain intravenous (i.v.) access in cardiac arrest victims. while several drugs can be administered via the endotracheal (et) route, serum drug levels are lower than those obtained with the i.v. route. the authors hypothesized that a 90-degree torso tilt after et drug administration would increase drug levels.19979709362
[protective effect of labetalol on cardiovascular consequences of brain death in the swine].assessment of the preventive effect on cardiovascular changes following experimental brain death (bd) in the pig by pretreatment with labetalol, an alpha and beta adrenoreceptor blocking agent.19979686073
stimulated acoustic emission nonbackscatter contrast effect of microbubbles seen with harmonic power doppler imaging.transient imaging has been introduced to enhance the signal intensities when using echo contrast agents. however, this phenomenon is not clearly understood. to evaluate the mechanisms of this phenomenon, isolated pig hearts were investigated with different echo imaging techniques in the beating, working heart as well as in an asystolic state without any motion of the heart. the hearts of five german farm pigs (21 +/- 2.5 kg) were surgically explanted and inserted in an artificial circulation pro ...199711174925
two cases of reciprocal translocations in domestic pigs producing small litters.summary: two boars of finnish yorkshire breed were selected for chromosomal analysis because of their small litters. one had a complex three breakpoint reciprocal translocation where the small distal chromosome segments were fused in new combinations between autosomes 2,9 and 14. this boar with a 2n = 38,xy,t(2;9;14)(q23;q22;q25) karyotype had produced approximately 3.3 piglets per litter and its fertility was reduced by 70% compared to average of litter size in finnish yorkshire breed pigs. in ...199721395832
differential expression of platelet-derived growth factor receptors in porcine fibroblasts cultured from skin and granulation tissue.this study investigated the biological response of fibroblasts cultured from uninjured skin and granulation tissue from different stages of healing wounds to the three isoforms of platelet-derived growth factor. fibroblasts were derived by explant culture from the skin or the granulation tissue that formed within open mesh nylon schilling-hunt chambers (postoperative days 10, 20, 30, and 50) which had been implanted subcutaneously in the backs of domestic pigs. cells were cultured under identica ...199617177826
removal of necrotic tissue with an ananain-based enzyme-debriding preparation.an enzymatic debriding preparation was formulated with purified enzyme derived from a crude pineapple stem extract. the primary component of this preparation was the sulfhydryl protease ananain which represented >/=85% of the proteolytic activity. the remaining proteolytic activity in the preparation was contributed by a co-purifying homologous cysteine protease comosain. taken together these two proteases provided a protein purity of greater than 95% as judged by sodium dodecyl sulfate polyacry ...199617309694
experimental studies on the pathway for migration and the development of taiwan taenia in domestic pigs.the pig is the most favorable experimental intermediate host of taiwan taenia. cysticerci in infected pigs are located in the liver except for a few extrahepatic ones. the present study was designed to investigate the pathway of migration of the oncospheres of taiwan taenia in the pig. in the first group, each of 5 small-ear-miniature (sem) and one landrace-small-ear-miniature (l-sem) pigs were injected with 5000 hatched oncospheres into the ear vein. three sem and one l-sem pigs were found to h ...19969198595
spectral analysis of ventricular fibrillation and closed-chest cardiopulmonary resuscitation.this study was designed to assess the interference by closed-chest cardiopulmonary resuscitation (cpr) on the ventricular fibrillation (vf) ecg signal in a porcine model of cardiac arrest and to elucidate which variable of vf spectral analysis reflects best myocardial blood flow and resuscitation success during cpr. fourteen domestic pigs were allocated to receive either 0.4 u/kg vasopressin (n = 7) or 10 ml saline (n = 7) after 4 min of vf and 3 min of cpr. using radiolabeled microspheres, myoc ...19969025132
[social dominance and rank relationships in domestic pigs: a critical review].viewing dominance as an attribute of repeated agonistic interactions between two individuals, the present paper reviews theoretical approaches towards concepts of dominance, methods of measurement, and basic principles and problems connected with social dominance in domestic pigs. domestic pigs are able to establish social organization structures during all stages of their ontogeny. according to definition, dominance relationships occur when a consistent asymmetry of the result of dyadic agonist ...19968999780
[recent data on the epidemiology of japanese encephalitis].japanese encephalitis is an arbovirosis the incidence and geographic distribution of which are increasing in rural areas of tropical and temperate asia. a total of about 45,000 to 50,000 clinical cases occur annually. the flavivirus responsible for the disease shows birds as usual hosts, and culex mosquitoes as vectors. after a first amplification cycle in birds, the virus can be transmitted to domestic pigs, then to man. this scheme, however, shows large variations in the different regions, acc ...19968991613
chromosome homology between the domestic pig and the babirusa (family suidae) elucidated with the use of porcine painting probes.homology among three pairs of domestic pig (sus scrofa) and five pairs of babirusa (babyrousa babyrussa) autosomes has been demonstrated with the use of porcine painting probes. with the results of this study, in addition to data obtained earlier through the application of banding techniques, correspondence between all individual chromosomes of these two distantly related pigs has been identified.19968995485
[basic values of blood coagulation parameters in pigs (sus scrofa domesticus)].on 23 clinical healthy pigs (2-4 months of age, body weight 13-42 kg) under ketamin-pentobarbital anaesthesia blood plasma coagulation parameters have been investigated. to obtain basic values 26 parameters were measured: number of thrombocytes, parameters of thrombelastogram and resonance-thrombogram, prothrombin time, activated partial thromboplastin time, thrombin time, reptilase time, factors i, ii, v, vii, viii, x, antithrombin iii, plasminogen, alpha 1-antitrypsin, alpha 2-antiplasmin, alp ...19968593155
the effect of prolonged pneumoperitoneum on renal function in an animal model.prolonged, increased intra-abdominal pressure (iap) during laparoscopic surgery has been associated with oliguria and anuria.19968605555
myocardial cell death in reperfused and nonreperfused myocardial infarctions. mr imaging with dysprosioum-dtpa-bma in the pig.to investigate whether dy-dtpa-bma-enhanced mr imagining would permit identification of myocardial cell death, myocardial infarction was induced in 12 domestic pigs.19968611317
structure and organization of pig mhc class ii drb genes: evidence for genetic exchange between loci.the pig major histocompatibility complex drb genes were studied by polymerase chain reaction (pcr) amplification of exon 2 from eight domestic pigs and two european wild boars. sequence comparisons together with a phylogenetic analysis showed the existence of at least three drb genes of which only one appears to be expressed. the two putative drb pseudogenes contained deletions in exon 2, making it possible to confirm the presence of three non-allelic drb genes by analyzing the length polymorphi ...19968613138
hyperglycemia results in an increase in myocardial interstitial glucose and glucose uptake during ischemia.the purpose of this investigation was to assess the effects of hyperglycemia, in the absence of changes in plasma insulin and arterial free fatty acid (ffa) levels, on interstitial glucose levels and glucose uptake across the left ventricular wall during ischemia in domestic swine. insulin secretion was suppressed with a continuous infusion of somatostatin. arterial ffa levels remained stable due to the suppression of insulin. microdialysis probes were used to estimate changes in interstitial gl ...19968622595
iced temperature injectate for thermodilution cardiac output determination causes minimal effects on cardiodynamics.controversy exists regarding the ideal injectate temperature for measuring cardiac output. iced temperature injectate gives a higher signal/noise ratio and less variability in the measured cardiac output. thus, less volume and fewer measurements are required. advocates of room temperature injectate have suggested that iced temperature injectate may perturb cardiodynamics. this concern has remained largely untested. to help resolve this controversy, we examined the effects of 5 ml iced injectate ...19968625640
comparison of self-expanding polyethylene terephthalate and metallic stents implanted in porcine iliac arteries.comparison of the biocompatibility of self-expanding polyethylene terephthalate (pet) stents with self-expanding metallic stents (wallstents).19968661640
controlled trial of laparoscopic-assisted vs open colon resection in a porcine model.several series of laparoscopic colon resection have been reported in the literature with varied results; however, no controlled series of laparoscopic vs open colon resection has been reported. the purpose of this study was to determine the relative safety and adequacy of laparoscopic colon resection in a controlled trial using a porcine model.19968662429
radiolabeled iododeoxyuridine: safety evaluation.the emphasis of radiolabeled iododeoxyuridine (*iudr) research at our institution to date has been to assess its safety as a potential therapeutic agent. toward this goal, we have performed preclinical and clinical studies, using various routes of administration, to detect adverse changes in normal tissues in both humans and animals. as iudr is rapidly dehalogenated by the liver, the intravenous route is unlikely to be successful in therapeutic efforts. we have therefore focused our attention on ...19968676196
splanchnic and peripheral tissue perfusion in experimental fat embolism.to investigate the acute effects of experimental fat embolism on splanchnic and peripheral perfusion and oxygenation in pigs.19968681568
myocardial dysfunction after successful resuscitation from cardiac arrest.to investigate the functional and metabolic changes in the myocardium after successful resuscitation from cardiac arrest.19968681604
experimental application of controlled limb reperfusion after incomplete ischaemia.severe local and systemic complications may occur after revascularization of extremities exposed to prolonged complete or incomplete ischaemia. these complications may be reduced by controlling the reperfusate and the circumstances of the reperfusion period. ten adult german domestic pigs were exposed to 6 h of incomplete limb ischaemia by occlusion of the left iliac artery. to simulate the clinical situation of embolectomy, the occlusive snares were released after the ischaemic period in five p ...19968696746
recent fusion events during evolution of pig chromosomes 3 and 6 identified by comparison with the babirusa karyotype.the chromosomes of the babirusa, a species considered to have diverged from an ancestor of the pig during the miocene epoch, about 12-26 million years ago, were studied to determine the sites of recent rearrangements during evolution of the domestic pig. it is shown that there is a pericentric inversion of the entire short arm on pig chromosome 1, compared to its counterpart in the babirusa (chromosome 15). we also present evidence suggesting that pig chromosome 3 was derived by a telomere-centr ...19968697808
sustained-release local hirulog therapy decreases early thrombosis but not neointimal thickening after arterial stenting.adventitial heparin delivery has been shown to inhibit thrombosis and neointimal thickening in a rat carotid injury model. to determine whether sustained, local delivery of hirulog, a potent antithrombin agent, inhibits thrombus formation and neointimal thickening after arterial stenting, silicone polymers containing hirulog were formulated at a concentration of 5.8% by weight and were tested in vitro to determine the rate of drug release. an oversized metallic stent was implanted in the carotid ...19968579010
evaluation of the effects of autotransfusion of unprocessed blood on hemodynamics and oxygen transport in anesthetized pigs.to examine the hemodynamic effects and the oxygen transport pattern of autotransfusion of unprocessed blood on hemodynamics and oxygen transportation.19968706465
endotoxic shock alters distribution of blood flow within the intestinal wall.to investigate whether a redistribution of blood flow from the mucosa to the muscular layer of the intestinal wall contributes to the observed increased arterial-mucosal pco2 gradient and the decreased mucosal tonometric ph during endotoxic shock.19968706490
effects of graded doses of vasopressin on median fibrillation frequency in a porcine model of cardiopulmonary resuscitation: results of a prospective, randomized, controlled trial.to assess the effects of graded doses of vasopressin vs. saline on median fibrillation frequency and defibrillation success in a porcine model of cardiopulmonary resuscitation.19968706492
four alginate dressings in the treatment of partial thickness wounds: a comparative experimental study.alginate-based absorption materials for wound treatment are meeting with increasing clinical acceptance. the purpose of this study was to compare four different calcium alginate dressings (algosteril, comfeel alginate, kaltostat and sorbsan) with respect to wound fluid retaining ability, adherence, dressing residues, epithelialisation and inflammatory cell infiltration using a standardised partial-thickness wound model in domestic pigs. wound fluid spread laterally onto surrounding normal skin b ...19968733355
myocardial dysfunction after resuscitation from cardiac arrest: an example of global myocardial stunning.this study investigated the effect of prolonged cardiac arrest and subsequent cardiopulmonary resuscitation on left ventricular systolic and diastolic function.19968752819
an epidemiological study of influenza viruses among chinese farm families with household ducks and pigs.to examine the possibility of interspecies transmission and genetic reassortment of influenza viruses on farms in southern china, we surveyed 20 farm families living outside the city of nanchang who raised pigs and ducks in their homes. weekly interviews of family members and virus isolation studies of throat swabs and faecal samples, collected from september 1992 to september 1993, established the seasonal pattern of respiratory tract infections in these families and identified 11 influenza vir ...19968760967
age dependence of cerebrovascular response mechanisms in domestic pigs.hypercapnia-induced cerebral vasodilation in the newborn pig is a prostanoid-associated response. in some adult models, hypercapnic cerebral vasodilation is associated with the generation of nitric oxide (no). acetylcholine (ach) produces a no-dependent cerebral vasodilation in many adult models, but topical ach is a prostanoid-associated cerebral vasoconstrictor in the newborn pig. we hypothesized that mediators influencing cerebral response can be age dependent. juvenile domestic pigs were com ...19968770094
myocardial adaptation during and after sustained, demand-induced ischemia. observations in closed-chest, domestic swine.we tested the hypotheses that prolonged, demand-induced myocardial ischemia plateaus and that on relief of stress, myocardial function remains depressed, with proportionate reductions in blood flow and oxygen consumption indicative of hibernation.19968772699
a porcine model of chronic peripheral arterial occlusion.to create a simple and reproducible model of chromic thrombosis for the evaluation of thrombolytic agents and devices.19968773977
electromyographic and mechanomyographic characteristics of neuromuscular block by magnesium sulphate in the pig.in spite of its well known propensity to cause accidental paralysis by a specific mechanism of action, mg(2+)-induced neuromuscular block has not been examined systematically for its characteristics of muscle response to nerve stimulation. we examined in seven anaesthetized domestic pigs the mechanomyographic (mmg) and neurally evoked compound electromyographic (ncemg, emg) responses of the tibialis anterior muscle to stimulation of its motor nerve, at baseline and during three levels of neuromu ...19968777111
effects of hypercholesterolaemia on physiological recruitment of coronary vascular reserve in swine.1. the endothelium participates in the regulation of coronary vascular tone. as evidence exists from studies performed on epicardial vessels that hypercholesterolaemia impairs endothelial function, we tested the hypothesis that hypercholesterolaemia impairs coronary vascular reserve in an intact animal. 2. domestic swine, maintained on a regular (n = 9) or a 2% high-cholesterol (n = 9) diet for 3 months were instrumented with a catheter in the left atrium for microsphere injection, a catheter in ...19968777832
alternative methods of exposure minimize cardiopulmonary risk in experimental animals during minimally invasive surgery.alternative methods of exposure are needed for minimally invasive surgery to avoid hypercarbia and acidosis associated with carbon dioxide (co2) abdominal insufflation. the goals of this study were to determine the pulmonary and hemodynamic effects of both helium (he) abdominal insufflation and placement of a mechanical abdominal wall-lifting device (lifter) during laparoscopy.19968779063
sus scrofa domestica endoparasitic resistance in the amazonas.the domestic pig (sus scrofa domestica l.) has been wild breeding in the varzea amazonian estuary area. without dietary supplement or medical prevention, the pigs survive, bringing economic benefits to local peasants. endoparasitic diseases generate economic losses because of neonatal death and clinical disease. we assessed the nematoda (o. nematoda) and coccidia (o. eucoccidia) parasitic level and tolerance in 75 pigs (0-18 months) from 35 families using the following methods: (i) larvae cultur ...19968784532
immunocytochemical distribution of catecholamine-synthesizing neurons in the hypothalamus and pituitary gland of pigs: tyrosine hydroxylase and dopamine-beta-hydroxylase.this study describes the distribution of catecholaminergic neurons in the hypothalamus and the pituitary gland of the domestic pig, sus scrofa, an animal that is widely used as an experimental model of human physiology in addition to its worldwide agricultural importance. hypothalamic catecholamine neurons were identified by immunocytochemical staining for the presence of the catecholamine synthesizing enzymes, tyrosine hydroxylase and dopamine-beta-hydroxylase. tyrosine hydroxylase-immunoreacti ...19968789282
effects of intracoronary carbon dioxide on left ventricular function in swine.carbon dioxide is currently used as an arterial and venous contrast agent; however, little is known of its effects on left ventricular function. this study was undertaken to investigate those effects.19968790949
increased concentrations of endothelin-1 messenger rna in tissues and endothelin-1 peptide in plasma in septic pigs: modulation by betamethasone.to study the expression of preproendothelin-1 messenger rna (mrna) in tissue after escherichia coli lipopolysaccharide challenge and to evaluate the possible effects of betamethasone both regarding endothelin-1 production as well as hemodynamic and vascular effects during e. coli lipopolysaccharide infusion in pigs in vivo.19968797627
heritability estimate of hypertrophic cardiomyopathy in pigs (sus scrofa domestica).the purpose of this study was to evaluate the heritability of hypertrophic cardiomyopathy (hcm) in pigs and the relation between hcm and heart measurements, pathologic features, and growth to provide references for hcm line development. a total of 353 on-farm tested gilts (females) and boars (males) from 74 sire families were randomly selected from a single breeding farm where hcm was prevalent. hearts were collected after animals were slaughtered. heart length, width, and weight, heart-to-body ...19968799938
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