Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| purification of arginine-sensitive hemagglutinin from fusobacterium nucleatum and its role in coaggregation. | hemagglutinin of fusobacterium nucleatum was extracted from triton x-100-pronase p-treated cell envelopes, and was purified by affinity chromatography on l-arginine agarose. the hemagglutinin was inactivated by heating at 70 degrees c for 1 min. the activity was inhibited by l-arginine but was not affected by any sugars or by edta. the hemagglutinin aggregated 14 out of 17 strains of oral streptococci tested, and the bacterial aggregating activity was also inhibited by l-arginine. the results in ... | 1993 | 8426279 |
| sub-gingival microflora in macaca mulatta species of rhesus monkey. | the macaca mulatta species of rhesus monkey is one of several non-human primate (nhp) models for periodontal disease. this report presents the bacteriology of the gingival sulci in m. mulatta monkeys. three sub-gingival sites (maxillary right central incisor, the disto-buccal of the mandibular left second molar and mesio-buccal of the mandibular right second molar) of 9 monkeys were evaluated clinically before scaling and 7 days after scaling. plaque samples were obtained from sub-gingival sites ... | 1993 | 8426283 |
| phagocytosis of bacterial strains isolated from acute dentoalveolar abscess. | the phagocytosis by human polymorphonuclear leucocytes of 37 bacterial strains identified as streptococcus milleri (10 strains), strictly anaerobic gram-positive cocci (10) prevotella intermedia (6), pr. oralis (5) and fusobacterium nucleatum (6) was investigated in vitro. the ingestion of s. milleri and strictly anaerobic gram-positive cocci was significantly greater (p < 0.001) than that of strains of prevotella spp. and f. nucleatum. the degree of uptake of capsulate and non-capsulate strains ... | 1993 | 8429541 |
| a 6-month home usage trial of a 1% chlorhexidine toothpaste (ii). effects on the oral microflora. | the effects of 1% chlorhexidine and 1% chlorhexidine/fluoride dentifrices on supragingival plaque flora were investigated in a 6-month home-usage study. 146 subjects were studied from whom plaque around 6 teeth was collected at baseline, 6 weeks and 24 weeks. total anaerobic counts were reduced at 6 and 24 weeks in both active groups compared to control. no differences were seen in any other bacterial parameters. there was a significant decrease in the susceptibility to chlorhexidine of bacteria ... | 1993 | 8450086 |
| the effect of subgingival debridement on periodontal disease parameters and the subgingival microbiota. | the aim of the present investigation was to analyse the effect of subgingival scaling and root planing in subjects who prior to treatment exercised meticulous supragingival plaque control. 300 subjects were examined at baseline and after 1 and 2 years without treatment. after the year 2 examination, 62 subjects were randomly selected for therapy. they were given detailed instruction in proper self-performed toothcleaning measures and were carefully monitored during the subsequent 2 years. follow ... | 1993 | 8501276 |
| methylation of adenine and cytosine in some strains of fusobacterium nucleatum. | dna from six strains of fusobacterium nucleatum was examined for possible methylation of adenine or cytosine in the restriction site gatc. f. nucleatum dna was digested with the restriction enzymes sau3ai, dpni and mboi, subjected to electrophoresis in agarose gels and southern blotting. the blots were hybridized with a polymerase chain reaction (pcr)-amplified dna probe. both radioactive and non-radioactive labeling methods were used. some strains of f. nucleatum were found to be dam methylated ... | 1993 | 8502161 |
| microbial colonization of dental implants in partially edentulous subjects. | this study assessed the colonization of brånemark dental implants by periodontopathic bacteria in four partially edentulous patients with a total of 10 implants. marginal and subgingival plaque on the implants was sampled 14 days and 28 days after second-stage surgery and compared with plaque from three teeth closest to the implant sites sampled before second-stage surgery (baseline) and at 14 and 28 days. slot immunoblot assay was used to determine the presence or absence of bacterial antigen t ... | 1993 | 8396644 |
| human monocytes and gingival fibroblasts release tumor necrosis factor-alpha, interleukin-1 alpha and interleukin-6 in response to particulate and soluble fractions of prevotella melaninogenica and fusobacterium nucleatum. | in this study we provide evidence that structural and soluble components of periodontopathogenic bacteria, such as prevotella melaninogenica and fusobacterium nucleatum, induce the release of cytokines in vitro known to cause in vivo necrotic inflammatory phenomena and bone resorption (tumor necrosis factor-alpha, interleukin-1 alpha and interleukin-6). human monocytes and gingival fibroblasts were cultivated in vitro in the presence of both particulate and soluble bacterial fractions. a dose-de ... | 1993 | 8400338 |
| localization of the fusobacterium nucleatum t18 adhesin activity mediating coaggregation with porphyromonas gingivalis t22. | adherence of pathogenic bacteria is often an essential first step in the infectious process. the ability of bacteria to adhere to one another, or to coaggregate, may be an important factor in their ability to colonize and function as pathogens in the periodontal pocket. previously, a strong and specific coaggregation was demonstrated between two putative periodontal pathogens, fusobacterium nucleatum and porphyromonas gingivalis. the interaction appeared to be mediated by a protein adhesin on th ... | 1993 | 8380804 |
| late onset papillon-lefèvre syndrome? a chromosomic, neutrophil function and microbiological study. | we had the opportunity to study a family with one of the most destructive forms of periodontal disease known, the papillon-lefèvre syndrome. the parents had no consanguinity and were not affected, and were therefore to be considered carriers of the disease. 2 sisters, the eldest and youngest, showed periodontal breakdown and hyperkeratotic skin lesions, but their deciduous dentition was not affected. 2 brothers had skin lesions only and another brother and sister were healthy. furthermore, 2 bab ... | 1993 | 8227454 |
| complete sequence of omp1, the structural gene encoding the 40-kda outer membrane protein of fusobacterium nucleatum strain fev1. | the sequence of the omp1 gene coding for the 40-kda outer membrane protein (omp) of the gram- oral bacterium, fusobacterium nucleatum strain fev1, has been determined. degenerate oligodeoxyribonucleotide primers were used to prime the amplification of a 120-mer sequence of the gene. this sequence was successively used for constructing new primers applied in asymmetrical, symmetrical, and inverse polymerase chain reaction using as template genomic dna, self-ligated dna fragments, or fragments lig ... | 1993 | 8406032 |
| prb3 null mutations result in absence of the proline-rich glycoprotein gl and abolish fusobacterium nucleatum interactions with saliva in vitro. | the glycosylated proline-rich glycoprotein (gl or prg), a product of the prb3 gene, is a major constituent of human parotid saliva. important functions proposed for gl include acting as a bacterial receptor. the gl proteins of several subjects were typed by two polyacrylamide gel electrophoresis (page) systems: acid-lactate page followed by staining with the periodic acid-schiff reagent and sodium dodecyl sulfate-page followed by electrophoretic transfer and staining with amido black or concanav ... | 1993 | 8406834 |
| lethal photosensitization of bacteria in subgingival plaque from patients with chronic periodontitis. | subgingival plaque samples from patients with chronic periodontitis were exposed to light from a 7.3 mw helium/neon laser for 30 s in the presence and absence of 50 micrograms/ml toluidine blue o as a photosensitizer. viable counts of various groups and species of bacteria were carried out before and after irradiation. the median numbers of viable bacteria initially present in the 30-microliters aliquots irradiated were 1.13 x 10(5) cfu (aerobes), 4.08 x 10(5) cfu (anaerobes), 4.92 x 10(3) cfu ( ... | 1993 | 8388448 |
| phosphorylated nutrient uptake by treponema denticola and other potential periodontopathogens. | nutrient uptake may contribute to the survival of certain potential periodontopathogens in the competitive environment of the gingival sulcus. this study was conducted to assess the uptake of commercially available, key radiolabelled phosphorylated compounds by treponema denticola. there was a linear relationship in the uptake of atp and g-1-p with cell concentrations of 0.1-2.5 mg of cell dry weight. this uptake reached a steady state within 15 min, and at temperatures of 25-37 degrees c the up ... | 1993 | 8229676 |
| serum immunoglobulin g antibodies to porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum and streptococcus sanguis during experimental gingivitis in young adults. | twenty-eight young, healthy adults completed an experimental gingivitis study in which blood and clinical recordings were obtained at baseline; after a 4-week period of thorough oral hygiene; after a subsequent 3-week period of plaque accumulation; and after another 2 weeks of thorough oral hygiene. serum immunoglobulin g antibodies against whole cells of porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum and streptococcus sanguis were determined using enzyme-linked immunos ... | 1993 | 8233568 |
| sensitization of periodontopathogenic bacteria to killing by light from a low-power laser. | cultures of porphyromonas gingivalis, fusobacterium nucleatum and actinobacillus actinomycetemcomitans were treated with a range of photosensitizers and then exposed to light from a 7.3 mw helium/neon laser for up to 80 s. toluidine blue o (25 micrograms/ml) and methylene blue (25 micrograms/ml) were effective lethal photosensitizers of all 3 target organisms, enabling substantial light dose-related reductions in viable counts. dihaematoporphyrin ester and aluminium disulphonated phthalocyanine ... | 1993 | 8233573 |
| tetracycline resistance in periodontal pathogens. | antimicrobial agents are used in combination with debridement to eliminate putative periodontal pathogens, including porphyromonas gingivalis, prevotella intermedia, and actinobacillus actinomycetemcomitans, from diseased tissues. the most frequently used antimicrobial agents are the tetracyclines. however, these agents are not effective in some patients. this lack of efficacy may be due to antimicrobial resistance. as many as 75% of the bacteria in the subgingival flora may be resistant to tetr ... | 1993 | 8324137 |
| microbial associations in periodontitis sites before and after treatment. | duplicate samples from 110 periodontal sites of 6 mm or more pocket depth in 16 patients were analyzed for the presence of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, capnocytophaga spp., campylobacter rectus, eikenella corrodens and fusobacterium nucleatum. the sites were sampled before and after nonsurgical periodontal treatment. no statistically significant associations were found before treatment between any of the analyzed species. after treatment, ... | 1993 | 8247608 |
| investigating the source of amniotic fluid isolates of fusobacteria. | 1993 | 8324160 | |
| correlation between thermal sensitivity and microorganisms isolated from deep carious dentin. | the correlation between thermal sensitivity and the microorganisms present in 29 deep carious lesions was studied. the numbers of lactobacilli and total gram-positive rods in the carious lesions were found to be negatively related to the length of pain triggered by cold and heat stimulants. the presence of gram-positive cocci and non-black-pigmented bacteroides were positively associated with both cold and heat sensitivities. black-pigmented bacteroides, streptococcus mutans, and total anaerobic ... | 1993 | 8289023 |
| effects of antibiotic treatment on clinical conditions and bacterial growth with guided tissue regeneration. | mucogingival flaps were reflected over pairs of mandibular molar teeth with class ii furcation invasions. the dimensions of the furcations were measured. the teeth were debrided and an expanded polytetrafluoroethylene (e-ptfe) membrane was placed and retained over one furcation of each pair (test site) for 4 weeks. the second site served as a control. eight patients (group 1) with 12 e-ptfe sites received no antibiotic. seven patients (group 2) with 12 e-ptfe sites were administered amoxicillin/ ... | 1993 | 8366411 |
| bactericidal effects of povidone-iodine solution to oral pathogenic bacteria in vitro. | seven species of periodontal pathogenic bacteria (bacteroides, gingivalis, bacteroides intermedius, bacteroides melaninogenicus, fusobacterium nucleatum, actinobacillus actinomycetemcomitans, capnocytophaga spp., eikenella corrodens) and two control species (streptococcus intermedius, pseudomonas aeruginosa) were selected in order to study the bactericidal effects of 10% povidone-iodine (pvp-i) aqueous solution in vitro. the pvp-i solution was diluted to 10, 20, 50, 100, 200, 400, 800, 1600, 320 ... | 1993 | 8290445 |
| enzyme activity in crevicular fluid in relation to metabolic control of diabetes and other periodontal risk factors. | diabetics are generally considered at higher risk for periodontitis than non-diabetics. among diabetics, those with poorer metabolic control have often been found to have more periodontitis. this study investigated the relationship between two crevicular fluid enzymes, beta-glucuronidase (beta g) and lactic dehydrogenase (ldh), and metabolic control in 93 adults with type 1 or 2 diabetes. metabolic control was evaluated by glycosylated hemoglobin (hba1c) levels. the most visibly inflamed site wa ... | 1993 | 8390572 |
| measurement of electrical bioimpedance for studying utilization of amino acids and peptides by porphyromonas gingivalis, fusobacterium nucleatum, and treponema denticola. | the growth response of porphyromonas gingivalis, fusobacterium nucleatum, and treponema denticola to peptides (supplied as trypticase) and amino acids (supplied as casamino acids) was measured over 24 hours by monitoring changes in alternating-current conductivity at 37 degrees c. all species utilized peptides preferentially over amino acids. these results are consistent with those obtained previously with conventional growth-response experiments. the duration of the linear growth response to tr ... | 1993 | 8391864 |
| lethal photosensitization of oral anaerobic bacteria. | 1993 | 8391865 | |
| the influence of race and gender on periodontal microflora. | previous studies have demonstrated that demographic characteristics of subject populations influence both the incidence of periodontal diseases and various aspects of host responses to periodontal bacteria. in this study we analyzed the components of the subgingival microflora from individuals with adult periodontitis, early onset periodontitis, gingivitis, and periodontal health as a function of gender and race (black and white). clinical categories were analyzed individually so that there were ... | 1993 | 8387107 |
| macaca nemestrina: a non-human primate model for studies of periodontal disease. | the non-human primate macaca nemestrina was evaluated for use as a potential model in periodontal research by study of 16 animals. using one incisor, premolar, and molar per quadrant, we measured supragingival plaque, severity of gingival inflammation, and pocket depth, and analyzed the subgingival flora. serum igg titers and avidities to antigens of porphyromonas gingivalis (pg) and actinobacillus actinomycetemcomitans (aa) were also assessed. ten animals were between 13 and 24 years old, and s ... | 1993 | 8393106 |
| antiseptic mouthrinse-induced microbial cell surface alterations. | this study determined the effect of an essential oil-containing mouthrinse, listerine antiseptic, on microbial cell surface morphology using scanning electron microscopy. twenty-four hour cultures of c. albicans, a. viscosus, s. sanguis, f. nucleatum, and a. actinomycetemcomitans were harvested and washed in buffer. triplicate samples were overlaid on poly-l-lysine coated coverslips, immersed in either listerine or buffer for 30 seconds, fixed in glutaraldehyde followed by post-fixing in 1% osmi ... | 1993 | 7880474 |
| antibacterial effects of various endodontic medicaments on selected anaerobic bacteria. | this study was undertaken to determine the antibacterial effects of various endodontic medicaments against six selected anaerobic bacteria. the experiment involved the testing of the vapors of six medicaments using agar plates streaked with the bacteria. a zone of inhibition was recorded for each plate and the results were analyzed statistically. formocresol produced significantly larger zones of inhibition than any of the other medicaments. | 1993 | 8120484 |
| the antimicrobial activity of prevention mouthrinse. | prevention mouthrinse was designed to serve as a vital supplement to normal oral hygiene procedures. to determine the antimicrobial potency of this mouthrinse, minimal inhibitory concentrations (mics), bactericidal kinetics, and short-term exposure studies were conducted. a spectrum of oral microorganisms was employed in this investigation: streptococcus sanguis, streptococcus anginosus, streptococcus mutans, actinomyces viscosus, actinobacillus actinomycetemcomitans, fusobacterium nucleatum, ei ... | 1993 | 7880467 |
| new findings on the microbial flora associated with adult periodontitis. | the predominant microbial flora associated with adult periodontitis was investigated in 21 patients aged 20-40 years. microorganisms from samples of subgingival plaque were cultured on selective and non-selective media under aerobic and anaerobic conditions. ten species or groups out of a total of 31 were found to comprise the predominant cultivable flora: diphtheroids, porphyromonas asaccharolytica, coagulase-negative staphylococci, 'streptococcus milleri'-group, bacteroides ureolyticus, capnoc ... | 1993 | 8315093 |
| association between cigarette smoking, bacterial pathogens, and periodontal status. | the purposes of this study were to determine if: 1) an association exists between cigarette smoking and signs of periodontal disease after controlling for the confounding variables of age, sex, plaque, and calculus; 2) the prevalence of 5 bacteria commonly associated with periodontal disease differs between smokers and non-smokers; and 3) the presence of any of these bacteria or smoking are associated with a mean proximal posterior probing depth > or = 3.5 mm. plaque, calculus, gingivitis, and p ... | 1993 | 8106950 |
| a comparison of two transport media for saliva and subgingival samples. | this study has shown that microorganisms associated with caries (mutans streptococci, lactobacilli) and marginal periodontitis (porphyromonas gingivalis, prevotella intermedia and actinobacillus actinomycetemcomitans) in pure culture can be stored in vmga or reduced transport fluid and be recovered sufficiently after 24 h. sufficient recovery after 24 h of mail transport was also obtained in 37 saliva samples obtained from adults and 80 subgingival samples from a group of patients before and 3, ... | 1993 | 8152839 |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 43-1993. a 71-year-old woman with confusion, hemianopia, and an occipital mass. | 1993 | 8413415 | |
| polymerase chain reaction-amplified nonradioactive probes for identification of fusobacterium nucleatum. | a polymerase chain reaction probe with 100% sequence identity to 120 deoxyribonucleotides of fusobacterium nucleatum fev1, coding for a part of the 40-kda major outer membrane protein, was labeled with the steroid hapten digoxigenin. the probe was compared with various degenerate oligonucleotide probes and found to tolerate much more stringent washing conditions. it was therefore superior in distinguishing, by means of southern blots and slot blots, f. nucleatum from other oral gram-negative bac ... | 1993 | 8458947 |
| [principles of personnel management]. | 1993 | 187861 | |
| [double primary carcinomas of the esophagus and stomach: a pathological study of 9 cases (author's transl)]. | 1993 | 262814 | |
| [total arthroplasties in patients with hemophilia]. | 1993 | 3250801 | |
| project breakthrough: an evaluation of selected goals. | 1992 | 258193 | |
| [thoracic empyema caused by anaerobes. 78-month retrospective study. clinical and microbiological aspects]. | the authors considered the microbiological and clinical aspects of the thoracic empyemas caused by anaerobic bacteria observed at the ivrea-castellamonte hospital, turin, over a period of 78 months. in this period 321 patients were subjected to thoracentesis in order to take samples of the pleural fluid (pf) which was in turn subjected to culture investigations. 31 patients (9.6%) had one or more cultures which were positive for bacterial growth. a good 12 patients (38.7%) had cultures which wer ... | 1992 | 7498613 |
| [identification of bacteria using dna probes]. | since the microbial specificity of periodontal diseases is well established, having a valid microbial diagnostic test is essential for a correct and a coherent treatment planning. dna probe will be used to identify and quantify the oral pathogens, most commonly associated with periodontitis. this test utilizes innovative dna probe technology to identify unique segments of dna in each of the following bacteria: bacteroides gingivalis, bacteroides intermedius, actinobacillus actinomycetemcomitans, ... | 1992 | 12051174 |
| expression and release of neuroregulators during development: monoamines and neuropeptides. | the turnover of catecholamines (ca) was determined in the adrenal medulla and brain of rat fetuses and pups. in general we found a considerable increase soon after birth. the expression of mrna for ca-synthesizing enzymes was also considerably enhanced in the adrenals shortly after birth. furthermore, we demonstrated increased expression of neuropeptides after birth, increased synthesis of mrna encoding for neuropeptide y in the adrenals 24 h after birth; and considerable activation of the subst ... | 1992 | 1306802 |
| hypoxic-ischemic damage in the neonatal brain: excitatory amino acids. | perinatal brain damage is a major clinical problem. recent studies suggest that excitatory amino acids (eaas) may be important for the development of hypoxic-ischemic brain injury in the newborn. experimental work demonstrates that the immature brain is hypersensitive to the toxic effects eaa ('excitotoxicity'), hypoxic-ischemia is accompanied by an extracellular overflow of eaas and hypoxic-ischemic brain damage is reduced by eaa receptor antagonists. clinical investigations demonstrate the pre ... | 1992 | 1306803 |
| the effect of 3 mouthrinses on plaque and gingivitis development. | the aim of this study was to evaluate the effect of 3 mouthrinses, listerine antiseptic (thymol), peridex (chlorhexidine), perimed (povidone iodine and hydrogen peroxide), and a placebo (water) on the development of dental plaque and gingivitis, when used as the only oral hygiene procedure for 14 days. 71 subjects were entered into a randomized, double-blind study. at the baseline examination, papillary bleeding score (pbs), and plaque index (pi) were registered, after which subjects received su ... | 1992 | 1310096 |
| use of adeno-associated virus as a general transduction vector for mammalian cells. | 1992 | 1316261 | |
| dofetilide, a novel class iii antiarrhythmic agent. | dofetilide is a potent and selective class iii antiarrhythmic agent that is under development for the treatment of re-entrant tachyarrhythmias (ventricular tachycardia/ventricular fibrillation, atrial fibrillation/atrial flutter, and paraoxysmal supraventricular tachycardia). in animal studies, dofetilide selectively inhibits the rapid component of the time-dependent outward potassium current (ikr) and therefore increases the effective refractory period and action potential duration without affe ... | 1992 | 1279316 |
| sensitization of oral bacteria in biofilms to killing by light from a low-power laser. | biofilms of streptococcus sanguis, porphyromonas gingivalis, fusobacterium nucleatum and actinobacillus actinomycetemcomitans were prepared on the surfaces of agar plates and a number of compounds were screened for their ability to sensitize bacteria in these biofilms to killing by light from a 7.3 mw helium/neon (he/ne) laser. toluidine blue o and methylene blue enabled detectable killing of all four target organisms after exposure to he/ne light for 30 s. aluminium disulphonated phthalocyanine ... | 1992 | 1334649 |
| bacterial concentration fluorescence immunoassay (bcfia) for the detection of periodontopathogens in plaque. | a bacterial concentration fluorescence immunoassay (bcfia) was developed to rapidly detect periodontopathic bacteria in human plaque samples. the bcfia utilized fluorescent-tagged monoclonal antibodies (mabs) directed against the lipopolysaccharide of selected gram-negative bacteria. microorganisms identified in plaque using the bcfia included actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, and fusobacterium nucleatum. the immunoassay procedure involved com ... | 1992 | 1336047 |
| cd45ra and cd45ro positive cd4 cells in human peripheral blood and periodontal disease tissue before and after stimulation with periodontopathic bacteria. | flow cytometric analysis was used to examine naive and primed or memory cd4 cells extracted from periodontal lesions compared with cells from peripheral blood of healthy subjects before and after stimulation with the periodontopathic bacteria, porphyromonas gingivalis and fusobacterium nucleatum. in peripheral blood, approximately 60% and 40% of cd4 cells were cd45ro+ and cd45ra+ respectively at day 0. phytohaemagglutinin (pha) induced cd45ro expression on almost 100% of cd4 cells. however, p. g ... | 1992 | 1356262 |
| susceptibility of anaerobic microorganisms to hypothiocyanite produced by lactoperoxidase. | the susceptibility of capnocytophaga ochracea, eikenella corrodens, eubacterium yurii, fusobacterium nucleatum, peptostreptococcus micros, prevotella intermedia, selenomonas sputigena, wolinella recta to hypothiocyanite (oscn-) produced by the lactoperoxidase system was tested. results showed a decrease of bacterial survival rate after oscn- exposure, with an intra- and inter-species variability from 0 to 95% for c. ochracea, 34-100% for e. corrodens, 0-83% for e. yurii, 1-15% for f. nucleatum, ... | 1992 | 1481764 |
| analyzing phenotypic correlations in studies with selected lines. | 1992 | 1317160 | |
| new monoclonal antibodies for the detection of immediate early antigens of cytomegalovirus. | two new monoclonal antibodies, cie-1 and cie-2, were developed for the rapid detection of human cytomegalovirus (hcmv) infection. they were found to be reactive with immediate early protein of hcmv in the nuclei of infected fibroblasts, as early as 3 hours post-infection. by radioimmunoprecipitation, cie-1 was found to react with a protein with an apparent molecular weight of 70,000, whereas cie-2 precipitated 2 proteins of 70,000 and 72,000 daltons, respectively. both monoclonal antibodies reco ... | 1992 | 1319172 |
| subgingival temperature (iii). relation to microbial counts. | the present investigation examined the relationship of selected bacterial species and subgingival temperature. 35 subjects were measured at 6 sites per tooth for clinical parameters and subgingival temperature. measurements were repeated for 21 subjects at 2 month intervals providing a total of 66 subjects visits. at each visit, subgingival plaque samples were taken from the mesial aspect of each tooth and anaerobically dispersed, diluted and plated on non-selective media. after anaerobic incuba ... | 1992 | 1321846 |
| different responses in b cells induced by porphyromonas gingivalis and fusobacterium nucleatum. | a phenotypic study had shown that gingival b cells respond differently to two periodontopathic bacteria, porphyromonas gingivalis and fusobacterium nucleatum. further investigation now shows a reduction in the percentage of ki-67 + t cells in cultures of gingival and peripheral blood mononuclear cells stimulated with p. gingivalis for 3 and 6 days, respectively, but no suppression of ki-67 expression in b cells in response to either p. gingivalis or f. nucleatum. depletion studies of cultures of ... | 1992 | 1359860 |
| structure of holo-chaperonin studied with electron microscopy. oligomeric cpn10 on top of two layers of cpn60 rings with two stripes each. | a structural model of holo-chaperonin, known as a protein-folding control protein comprising 60 kda (cpn60) and 10 kda polypeptides (cpn10), is proposed based on the electron microscopic images of holo-chaperonin from thermus thermophilus and cpn60 from paracoccus denitrificans. isolated paracoccus cpn60 shows very similar images to those of escherichia coli tetradecameric cpn60, a seven-membered ring in the top view and a rectangular shape with four stripes in the side view. however, a small nu ... | 1992 | 1347504 |
| sensitization of oral bacteria to killing by low-power laser radiation. | twenty-seven compounds were screened for their ability to sensitize streptococcus sanguis to killing by light from a 7.3-mw helium/neon (hene) laser. bacteria were mixed with various concentrations of the test compounds, spread over the surfaces of agar plates, and then exposed to light from the hene laser for various time periods. the plates were then incubated and examined for zones of inhibition. those compounds found to be effective photosensitizers were then tested against porphyromonas gin ... | 1992 | 1369193 |
| immunoglobulins in milk from cows immunized with oral strains of actinomyces, prevotella, porphyromonas, and fusobacterium. | immunization of pregnant cows with bacteria leads to the presence of high concentrations of specific antibodies in colostrum and milk. a total of 14 cows was immunized with single strains of heat-killed oral bacteria or pools of strains of actinomyces, porphyromonas, prevotella, and fusobacterium. two cows were treated with adjuvant alone. the mean percentages of igg1, igg2, igm, and iga in all of the milks were 83.8, 3.8, 9.3, and 3.1, respectively. elisa and whole cell agglutination assays dem ... | 1992 | 1354674 |
| dinucleotide (ca/gt) repeat polymorphism in intron 17b of the cystic fibrosis transmembrane conductance regulator (cftr) gene. | we describe a polymorphic microsatellite (ivs17bca) in intron 17b of the cystic fibrosis transmembrane conductance regulator (cftr) gene. it consists of an 11 to 20 ca/gt dinucleotide repeat, located 424 bp from exon 17b. | 1992 | 1370810 |
| the use of dna probes to examine the distribution of subgingival species in subjects with different levels of periodontal destruction. | the present investigation examined the distribution of 14 subgingival species at a total of 2299 sites in 90 subjects with different levels of periodontal destruction. subgingival plaque samples taken from the mesial aspect of each tooth were anaerobically dispersed, diluted and plated on non selective media. after anaerobic incubation, colonies were lifted to nylon filters and specific species detected using digoxygenin-labeled whole chromosomal dna probes. the mean total viable count for all s ... | 1992 | 1318331 |
| [lemierre's syndrome with splenic abscesses]. | a week after onset of a pharyngo-tonsillitis a previously healthy 23-year-old man developed high fever (41.4 degrees c), leukocytosis (12,200/microliters) with marked shift to the left, thrombocytopenia (86,000/microliters) and increased transaminases (got 83 u/l, gpt 113 u/l). chest x-ray film suggested intrapulmonary abscesses with left-sided pleural effusion. the suspected diagnosis of "post-tonsillitis" septicaemia (lemierre's syndrome) was confirmed by demonstrating anaerobic, fusiform, gra ... | 1992 | 1396137 |
| longitudinal monitoring of humoral antibody in subjects with destructive periodontal diseases. | fifty-one subjects (16-61 years old) with evidence of prior destructive periodontal disease were monitored clinically and immunologically at bi-monthly intervals for up to 5 yr. periodontal disease activity, determined as new attachment loss, was detected in 33 of these subjects. only 4 of 51 subjects failed to show an elevated serum antibody level to any of the 18 subgingival species tested. the antibody level threshold established for periodontally healthy subjects was exceeded most often in d ... | 1992 | 1403580 |
| 2-hydroxyglutaryl-coa dehydratase from fusobacterium nucleatum (subsp. nucleatum): an iron-sulfur flavoprotein. | anaerobically prepared cell-free extracts from fusobacterium nucleatum contain 2-hydroxyglutaryl-coa dehydratase with a specific activity of 20 nkat mg-1. the enzyme was purified 24-fold to a specific activity of 480 nkat mg-1 by anion exchange chromatography, gel filtration and chromatography on blue-sepharose. the activity of the purified enzyme was strictly dependent on the reductant ti(iii)citrate and stimulated 25-fold by 0.15 mm atp and 5 mm mgcl2. atp is hydrolysed to adp during incubatio ... | 1992 | 1417419 |
| mannitol does not affect energy metabolism in forebrain ischemia. | mannitol has a beneficial effect on ischemic injury following a short-duration forebrain ischemic insult in rats. using the same animal model, we attempted to show that this effect of mannitol could be traced (via an improvement in cerebral blood flow) to a tempering of the collapse in the high-energy phosphates that occurs during the insult. a 10 min ischemic insult was induced by bilateral carotid artery occlusion followed by a reduction of the blood pressure to 50 mmhg through removal of bloo ... | 1992 | 1421096 |
| identification of proteases from periodontopathogenic bacteria as activators of latent human neutrophil and fibroblast-type interstitial collagenases. | activation of latent human fibroblast-type and neutrophil interstitial procollagenases as well as degradation of native type i collagen by supra- and subgingival dental plaque extracts, an 80-kda trypsinlike protease from porphyromas gingivalis (atcc 33277), a 95-kda chymotrypsinlike protease from treponema denticola (atcc 29522), and selected bacterial species commonly isolated in periodontitis was studied. the bacteria included were prevotella intermedia (atcc 25261), prevotella buccae (es 57) ... | 1992 | 1398963 |
| serum igg reactive with oral anaerobic microorganisms associated with infections of endodontic origin. | numerous species of bacteria have been implicated with infections of endodontic origin. the purpose of this study was to compare the levels of serum igg antibodies reactive with a panel of 10 oral anaerobic microorganisms implicated in infections of endodontic origin. serum samples were collected from 4 patient groups that included healthy patients without endodontic or periodontal disease, patients with chronic adult periodontal disease, patients with endodontic disease and patients with combin ... | 1992 | 1528630 |
| comparative mitogenicity and polyclonal b cell activation capacity of eight oral or nonoral bacterial lipopolysaccharides in cultures of spleen cells from athymic (nu/nu-balb/c) and thymic (balb/c) mice. | optimal stimulatory doses of purified phenol-water extracted lipopolysaccharides (lps) from 5 selected strains of 3 putative periodontopathogens (fusobacterium nucleatum, prevotella intermedia, and veillonella), 3 strains of 2 nonoral bacterial species (bacteroides fragilis and salmonella enteritidis), and pokeweed mitogen (pwm) induced significantly higher maximum mitogenic responses and polyclonal ig production in cultures of unfractionated spleen cells from nu/nu-balb/c (nude) than from balb/ ... | 1992 | 1528635 |
| polymorphism in the rd (d6s45) gene. | the rd (d6s45) gene in the class iii region of the hla major histocompatibility complex encodes a protein normally containing 24 consecutive basic-acidic dipeptide repeats. we determined the frequency of variations in the number of repeats by use of the polymerase chain reaction. of 107 subjects 7 (3.3%) carried genes encoding 22 or 23 repeats. there was no difference in the frequency of such polymorphisms between normal individuals and those with systemic lupus erythematosus, a disease associat ... | 1992 | 1534063 |
| complement activation by lipopolysaccharides purified from gram-negative bacteria isolated from infected root canals. | the purpose of the present study was to investigate the complement activation by lipopolysaccharides purified from porphyromonas (bacteroides) endodontalis, veillonella parvula, and fusobacterium nucleatum isolated from infected root canals. the rate of consumption of the c3t component of the complement increased remarkably when lipopolysaccharides of more than 10 micrograms were added. | 1992 | 1437068 |
| comparison of the effects of galactose and glucose on the ph responses of human dental plaque, salivary sediment and pure cultures of oral bacteria. | comparisons made in dental plaque in vivo demonstrated that galactose produces a significantly smaller decrease in ph than does glucose. in vitro studies with plaque, salivary sediment and pure cultures of oral bacteria done in the absence of intraoral factors such as flowing saliva confirmed this lesser acidogenicity of galactose. pure culture showed that most of the bacteria tested produce a moderate to large decrease with glucose but only a few do so with galactose; most produced a moderate t ... | 1992 | 1444892 |
| a comparative study of randomly amplified polymorphic dna analysis and conventional phage typing for epidemiological studies of listeria monocytogenes isolates. | the analysis of rapd profiles generated by pcr with a single 10-mer, hlwl74, was compared to bacteriophage susceptibility data for epidemiological typing of listeria monocytogenes strains. a total of 104 l. monocytogenes strains was screened, all from serogroup 1 or serotype 4b. of these, 53 had been isolated during 6 different listeriosis outbreaks. the remaining 51 strains were chosen randomly from our collection. a total of 38 rapd types were observed, although each epidemic group of strains ... | 1992 | 1448626 |
| immunohistochemical localization of gangliosides in enu-induced rat glioma. | biochemical studies have indicated that the structurally simple gangliosides, including gd3 and gm3, are major glycolipid components of glioma tissues. in order to clarify the localization of the gangliosides in ethylnitrosourea-induced rat glioma, an immunohistochemical study was performed using antibodies against gm1, gm3, and gd3. the results obtained in normal fetus, newborn, and adult rat brain, and also in human glioma, were compared. in fetal and newborn rat brain, gd3 was present mainly ... | 1992 | 1449052 |
| an unrecognized etiology for pyogenic hepatic abscesses in normal hosts: dental disease. | cryptogenic pyogenic hepatic abscesses are a diagnosis of exclusion. we have identified two patients with severe dental disease at the time of the diagnosis of their liver abscess. in both cases, oral flora was cultured from the abscess. unlike a previous report, both patients were immunocompetent. when compared with a group of patients with liver abscesses and diverticulitis, two differences were found. in contrast to the single abscesses seen in 10 of 10 patients with diverticulitis, the patie ... | 1992 | 1449134 |
| cellular fatty acids in fusobacterium species as a tool for identification. | identification of fusobacteria from clinical specimens currently requires analysis of metabolic end products by gas-liquid chromatography in addition to certain biochemical and enzymatic tests because of the relative biochemical inactivity of these bacteria. even the finding of pointed, thin gram-negative cells on gram-stained slides can no longer be relied on for identification of fusobacterium nucleatum, since at least four other species of fusobacteria have been seen to exhibit similar morpho ... | 1992 | 1452706 |
| fusobacterium nucleatum subsp. fusiforme subsp. nov. and fusobacterium nucleatum subsp. animalis subsp. nov. as additional subspecies within fusobacterium nucleatum. | using a variety of physiological, biochemical, and molecular systematic analyses, we have shown previously that there are four groups within the species fusobacterium nucleatum. two of these groups of strains correspond to the recently proposed taxa f. nucleatum subsp. nucleatum and f. nucleatum subsp. polymorphum. in this paper we show that the two remaining groups are distinct and formally propose that they should be recognized as f. nucleatum subsp. fusiforme (type strain, nctc 11326) and f. ... | 1992 | 1581188 |
| clonal dental pulp cells (rdp4-1, rpc-c2a) synthesize and secrete osteopontin (spp1, 2ar). | dental pulp cells play an important role in maintaining dental mineralized tissue throughout life. supplementary mineralization such as reparative dentin and pulp stone frequently occurs after primary dentin formation. dental pulp cells are thought to be closely associated with such mineralization. we found that clonal rat dental pulp cells, rdp4-1 and rpc-c2a, produce and secrete osteopontin, but do not synthesize phosphophoryn which is a major noncollagenous protein found in dentin. the dental ... | 1992 | 1472061 |
| golden hamster embryonic genome activation occurs at the two-cell stage: correlation with major developmental changes. | the earliest time of onset of embryonic genome activation in golden hamsters was investigated. the inhibition of transcription by alpha-amanitin (11 micrograms/ml) in cultured embryos resulted in a total arrest of development of early 2-cell embryos (26 hr post-egg activation); under similar conditions, immediate cleavage divisions of 1-, late 2-, 4-, and 8-cell embryos were not affected. electrophoretic analysis of [35s]methionine-labeled embryonic proteins showed that alpha-amanitin treatment ... | 1992 | 1497872 |
| rat sertoli and spermatogenic cells express a similar gene, and its product is antigenically related to an outer dense fiber-associated protein. | we have previously reported that a heterodimeric protein secreted by rat sertoli cells is antigenically related to a protein associated with outer dense fibers of the sperm tail. therefore, we have explored the possibility that sertoli and spermatogenic cells express a similar gene encoding a homologous protein. a sertoli cell heterodimeric protein cdna probe recognizes specific mrna in pachytene and round spermatids fractionated by centrifugal elutriation; however, this specific mrna was less p ... | 1992 | 1472368 |
| primary culture of human fallopian tube epithelial cells and co-culture of early mouse pre-embryos. | we have established a monolayer culture system for human fallopian tube epithelial cells. the cells were isolated from tubes using collagenase digestion, and were cultured in ham's f-10 supplemented with 15% fetal calf serum. the epithelial cells derived from culture were characterized using immunocytochemical staining and electron microscopy. these cells were stained with antikeratin and anti-epithelial membrane antigen, but showed no staining after treatment with antivimentin. electron microsc ... | 1992 | 1497873 |
| [botryomycosis caused by fusobacteria]. | in the anglo-american literature botrymycosis is described as a chronic cutaneous granulomatous reaction to bacterial infection, containing granules resembling the sulphur granules seen in actinomycosis. the diagnostic and therapeutic aspects are discussed with reference to a recently observed patient. fusobacterium nucleatum was isolated as the bacterial cause of the pathologic disorder. | 1992 | 1506205 |
| hiv education and health education in the united states: a national survey of local school district policies and practices. | to determine the extent to which hiv education and health education policies and practices are required by school districts in the united states, a national probability sample of public school districts was surveyed by mail in 1990. of 2,150 districts selected, 78.1% responded. hiv education was required by 66.9% of districts. of these, the percentage requiring hiv education increased by grade level from 29.7% in kindergarten to 82.3% in 7th grade, then declined to 37.3% by 12th grade. districts ... | 1992 | 1479838 |
| development and characterization of porphyromonas gingivalis-specific rat t-cell clones. | porphyromonas gingivalis has been implicated as a major pathogen in periodontitis. to determine the role of t cells in the regulation of this disease, a method was developed for the generation and characterization of rat t-cell clones with antigen specificity to p. gingivalis whole cells. the clones studied so far demonstrated a t-helper (th) phenotype w3/13+, w3/25+, ox8- and ox22-. these t-cell clones proliferated in vitro in response to p. gingivalis, but not to other bacteria (prevotella int ... | 1992 | 1281976 |
| [treatment and monitoring of primary hypothyroidism]. | treatment of primary hypothyroidism requires a long-term hormonal therapy with levothyroxine which restores a normal tsh value. in a series of 192 patients followed up for at least 3 years we tried to evaluate the variability of maintenance dosage and to analyse the factors predictive of replacement dosage. the l-t4 dosage varied from one individual to the other, from 0.6 to 3.2 micrograms/kg. the mean maintenance dosage (118 +/- 38 micrograms/day) was similar in men and women weight for weight, ... | 1992 | 1287777 |
| myocardial protection by retrograde cardioplegic perfusion in the presence of acute coronary artery obstruction. an experimental study. | to investigate retrograde delivery of cardioplegic solutions as a means of enhancing myocardial protection in the presence of coronary artery occlusion, a two-part experimental model was devised. in part 1 (in vitro) the possibility of retroperfusing the entire myocardium during acute occlusion of the left anterior descending artery (lad) was assessed. in part 2 (in vivo) acute lad occlusion was performed in dogs, and during 2 hours of aortic cross-clamping crystalline cardioplegic solution was ... | 1992 | 1287835 |
| characterization of guanosine diphospho-d-mannose dehydrogenase from pseudomonas aeruginosa. structural analysis by limited proteolysis. | alginate is believed to be a major virulence factor in the pathogenicity of pseudomonas aeruginosa in the lungs of patients suffering from cystic fibrosis. guanosine diphospho-d-mannose dehydrogenase (gdpmannose dehydrogenase, ec 1.1.1.132) is a key enzyme in the alginate biosynthetic pathway which catalyzes the oxidation of guanosine diphospho-d-mannose (gdp-d-mannose) to gdp-d-mannuronic acid. in this paper, we report the structural analysis of gmd by limited proteolysis using three different ... | 1992 | 1370473 |
| soft-tissue management through effective patient subgingival bacterial control. | 1992 | 1291435 | |
| biochemical and functional characterization of the leucocyte tyrosine phosphatase cd45 (cd45ro, 180 kd) from human neutrophils. in vivo upregulation of cd45ro plasma membrane expression on patients undergoing haemodialysis. | the biochemical and functional characterization, and the regulation of plasma membrane expression of the leucocyte tyrosine phosphatase cd45, have been investigated in neutrophils from healthy donors and patients undergoing haemodialysis. cd45 proteins of 180 kd and 130-150 kd were precipitated from neutrophils from both healthy subjects and haemodialysed patients. prolonged storing, as well as trypsin treatment of samples containing the 180-kd cd45 protein, generated the 130-150-kd polypeptides ... | 1992 | 1370931 |
| the establishment of human t cell lines reactive with specific periodontal bacteria. | human t cell lines (tcls) were obtained by stimulation of peripheral blood mononuclear cells (pbmcs) with the 2 periodontopathic bacteria, porphyromonas gingivalis fdc-381 and fusobacterium nucleatum fdc-263. after the first round of stimulation and rest, the cells responded specifically to the bacteria originally used to establish each line. throughout the culture period, the responsiveness of each of the tcls to their specific bacteria increased. phenotypic analysis of the tcls revealed hetero ... | 1992 | 1383912 |
| regeneration of an adult peripheral nerve preparation in culture. | the methods used to maintain the vagus nerve from the adult rat in culture and how regeneration is studied in this preparation are described. a hypothesis is presented on the triggering of the cell body reaction. it is suggested that this reaction is initiated by proteins synthesized in nonneuronal cells at the site of a nerve lesion. these proteins, referred to as regenerins, reach the nerve cell body by retrograde axonal transport, where they initiate the regeneration process. | 1992 | 1282333 |
| microbiology of healthy and diseased periodontal sites in poorly controlled insulin dependent diabetics. | a group of poorly-controlled insulin dependent diabetes mellitus (iddm) patients were examined in a cross-sectional design for total microbial levels, microbial incidence, and the percent levels of selected periodontal microorganisms. these organisms were selected on the basis of prior reports that associated them with either periodontal disease or health. one periodontally-healthy and one periodontally-diseased site were examined in each iddm patient. increased levels of the periodontal pathoge ... | 1992 | 1315389 |
| dna probe and enzyme analysis in periodontal diagnostics. | recent research in periodontology has indicated that a finite number of bacterial species are associated with periodontal disease. this has generated the hypothesis that periodontal disease is essentially an infection due to one or more of the putative periodontopathogens; i.e., the specific plaque hypothesis (sph). this microbial specificity paradigm has already changed the way researchers and some clinicians view periodontal disease. the clinician must heed several cautions signs, however, if ... | 1992 | 1336048 |
| experimentally induced infection by oral anaerobic microorganisms in a mouse model. | a mouse model was developed to study the abscessogenic potential of pure and mixed cultures of oral anaerobes associated with infections of endodontic origin. maximum subcutaneous lesions were seen on their backs between 3 and 14 d after inoculation. strains of fusobacterium nucleatum, peptostreptococcus anaerobius, and veillonella parvula were pathogenic in pure culture. a mixed culture of f. nucleatum with either porphyromonas gingivalis or prevotella intermedia was significantly more pathogen ... | 1992 | 1408362 |
| synergistic effect of pyrophosphate and sodium dodecyl sulfate on periodontal pathogens. | our previous studies have shown that pyrophosphate (ppi), the anticalculus component of tartar-control dentifrices, inhibits the growth of organisms associated with coronal and root surface caries. the purposes of this investigation were to: 1) determine if periodontal pathogens are similarly susceptible to the growth-inhibitory properties of ppi; and 2) determine if combinations of pyrophosphate-sodium dodecyl sulfate (ppi-sds) inhibit growth synergistically. porphyromonas gingivalis, actinobac ... | 1992 | 1324304 |
| role of porphyromonas gingivalis 40-kda outer membrane protein in the aggregation of p. gingivalis vesicles and actinomyces viscosus. | porphyromonas gingivalis, an important pathogen in periodontitis, produces extracellular vesicles that aggregate with actinomyces viscosus cells. a 40-kda outer membrane protein (omp)-coding gene from p. gingivalis was cloned and the protein was found to be localized in these vesicles. the recombinant 40-kda omp did not show aggregation activity. however, affinity-purified antibody against the recombinant protein significantly inhibited aggregation of p. gingivalis vesicles with a. viscosus cell ... | 1992 | 1329700 |
| effect of clavulanic acid, sulbactam and tazobactam on three different beta-lactamases from bacteroides uniformis, clostridium butyricum and fusobacterium nucleatum. | the effect of three beta-lactamase inhibitors, clavulanic acid, sulbactam and tazobactam used in clinical practice were compared for their activity against purified beta-lactamases from bacteroides uniformis, clostridium butyricum and fusobacterium nucleatum. the enzymes from b. uniformis and c. butyricum were produced in fermenters under controlled growth conditions and the enzyme from f. nucleatum was produced in batch cultures. purification of the beta-lactamases was achieved by anion-exchang ... | 1992 | 1331018 |
| antibacterial efficacy of root canal medicaments. | the antibacterial efficacy of four endodontic medicaments was studied in vitro. calcium hydroxide (calacept), camphorated parachlorophenol (cpcp), cresophene, and 2% iodine-potassium iodide (iki) were tested against four anaerobic microorganisms, streptococcus mutans, peptostreptococcus anaerobius, porphyromonus gingivalis and fusobacterium nucleatum, for 10 or 15 min. iki was effective only against f. nucleatum and p. gingivalis, and the others were effective against all four microorganisms. | 1992 | 1337555 |
| effect of beta-lactamase inhibitors on beta-lactamases from anaerobic bacteria. | three beta-lactamase inhibitors in clinical use--clavulanic acid, sulbactam and tazobactam--were investigated for their activity on beta-lactamases from bacteroides uniformis, clostridium butyricum and fusobacterium nucleatum. purification of the beta-lactamases was carried out by anion-exchange chromatography, gel filtration and fplc. the inactivation of beta-lactamase activity was determined spectrophotometrically with nitrocefin as substrate. various concentrations of the inhibitors were prei ... | 1992 | 1338315 |
| release of endogenous noradrenaline from the vascularly perfused rat stomach in vitro: modulation by pre- and postsynaptic adrenoceptors. | we developed an experimental in vitro model to detect a very small amount of endogenous noradrenaline (na) released from the rat gastric sympathetic nerve terminals. the stomach was perfused via celiac artery with modified krebs-ringer solution containing 10 mm pargyline and 0.1% bovine serum albumin at a constant flow of 4 ml/min. the right greater splanchnic (spl) nerve (preganglionic nerve of the gastric sympathetic nerve) was stimulated electrically with square-wave pulses of 2 msec duration ... | 1992 | 1346643 |
| correlation of haemagglutination activity with trypsin-like protease activity of porphyromonas gingivalis. | porphyromonas gingivalis is a gram-negative anaerobic bacterium associated with various forms of periodontal disease. several characteristics of p. gingivalis are thought to contribute to its pathogenicity; these include haemagglutination and trypsin-like protease activity. previous studies suggest an association between haemagglutination and trypsin-like protease activity of p. gingivalis. to investigate this, two complementary quantitative experimental approaches were taken. five independent m ... | 1992 | 1332663 |
| detubularization-induced contractile response change of the ileum following ileocystoplasty. | we reported previously that following ileocystoplasty the structure and pharmacologic response of the implanted ileum changes towards that of the bladder. specifically, the relaxation response to alpha adrenergic (methoxamine) and purinergic (atp) stimulation reverses to a contractile response one month after the ileal segment is surgically made part of the urinary bladder. the present study was designed to investigate possible signals for this change and also to determine whether bladder respon ... | 1992 | 1351953 |
| phenolic constituents in erythrina x bidwilli and their activity against oral microbial organisms. | five flavonoid compounds, including two new isoflavanones, were isolated from the root bark of erythrina x bidwilli. their structures were determined to be 2,10-di(gamma,gamma-dimethylallyl)-3,9-dihydroxypterocarpan (erythrabyssin ii), 6,8-di(gamma,gamma-dimethylallyl)-7,2',4'-trihydroxyisoflavanone (bidwillon a), 8-gamma,gamma-dimethylallyl-2',4'-dihydroxy-[6",6"-di-methylpyrano - (2",3":7,6)]isoflavanone (bidwillon b), 8-gamma,gamma-dimethylallyl-7,4'-dihydroxyisoflavone (8-gamma,gamma-dimethy ... | 1992 | 1464104 |