Publications

tumor-specific immunity induced by somatic hybrids. ii. elicitation of enhanced immunity against the parent plasmacytoma.	hybrid cells derived from fusion of a balb/c plasmacytoma (tepc-15) and l cells (c3h origin) were used to stimulate tumor-specific immunity against the parental plasmacytoma cells. live hybrid cells induced tumor-specific immunity against tepc-15 more effectively than mitomycin-treated hybrid or tepc-15 tumor cells. adoptive transfer of immunity with spleen cells of mice immunized with hybrid cells was also more effective than that with mitomycin-treated tumor cells. the immunity induced by the ...	2001	88483
quantitative succinate-dehydrogenase histochemistry. iii. variations in histochemical succinatedehydrogenase activity in different cross-sections of the same muscle fibre.	the variation in histochemical sdh-activity at different levels in the same muscle fibre was determined in muscle fibre cross-sections both by visual classification and quantitative determination of the formazan-deposits. this work resulted in a confirmation of the earlier micro-biochemical studies of spamer and pette (1977, 1979) and lowrey et al. (1978) that the activity of enzymes of the citric acid cycle is not homogeneously distributed in a muscle fibre over its entire length. in addition i ...	2001	93101
lifestyle adjustments in elderly patients after burn injury.	although survival of older burn victims has improved significantly in recent years, controversy remains over the care that should be given to this group and the functional recovery of survivors. to assess the impact of burn injury on lifestyle in the elderly, we conducted a mail-in survey among surviving patients ages 45 years or older who were treated in our burn center from 1978 to the middle of 1987. the survey asked questions about living arrangements, personal care, family relations, social ...	2001	1572857
perinatal mortality.		2001	93220
haploid cell cultures of drosophila melanogaster.		2001	98714
family life cycle resource center in the elementary school.		2001	98495
gas pocket causing pacemaker malfunction.	pacemaker malfunction was attributed to the increase in impedance to current flow caused by a pocket of air separating the anodal contact plate of a unipolar generator from the overlying skin. lack of capture was noted 20 hours after implantation. the malfunction was permanently corrected by bedside aspiration of the gas with a sterile syringe.	2001	95278
[disopyramide in the treatment of ventricular extrasystole in chronic chagas' disease].		2001	95160
morphological variation in oat-cell carcinoma of the bronchus.		2001	87879
diffuse melanosis and trichochromuria in malignant melanoma.	a previous finding of pronounced excretion of trichochromes in a patient with metastasizing melanoma and diffuse melanosis prompted this study on trichochromes in another patient with these two features. the patient now reported also excreted large amounts of trichochromes. iit is suggested that diffuse melanosis in metastatic melanoma patients is due to deposition of trichochromes in the tissue.	2001	93371
[diagnostic value of determining hexokinase activity of leukocytes in ischemic heart disease].		2001	92605
fluoroscopically guided thin needle aspiration biopsy of the abdomen and retroperitoneum.	the results of 49 transabdominal and translumbar thin needle percutaneous aspiration biopsies in patients suspected of having malignant disease are reported. a correct diagnosis was obtained in 40 cases. placement of the chiba needle was accomplished by fluoroscopically controlled biplane radiography during constrast examinations. no complications were encountered. the technique allows cytologic diagnosis of malignancy without high risk exploratory surgery, prolonged hospitalization, or delay of ...	2001	97993
long-term outpatient vasodilator therapy of congestive heart failure. consideration of agents at rest and during exercise.	increased left ventricular filling pressure and reduced cardiac output are two major hemodynamic deficits in pump failure. in patients with chronic heart failure, consequences of these hemodynamic deficits and diminished cardiac reserve are manifested initially during stress and eventually at rest. the purpose of therapeutic interventions include reduction of ventricular filling pressure increase in cardiac output and improvement in cardiac reserve. to achieve these goals, the hemodynamic effect ...	2001	99031
mechanism of action of beta-blockers in hypertension.		2001	14643
on the occurrence and prognosis of aphasia in patients with cerebral infarction.	of 338 consecutive patients with cerebral infarction, aphasia in the acute phase was found in 96. of these, 18 had totally recovered from aphasic speech disturbances when leaving the hospital. the patients were examined 2-8, in average 6 years after the onset of the illness. 39% of them had died, 27% still suffered from aphasic speech disturbances, while 32% were recovered from aphasia. in addition, disturbances in writing, reading, and/or calculation were observed in more than the half of the s ...	2001	95489
[current status of kidney transplantation and results].		2001	2860545
evidence for passive electrotonic interactions in red rods of toad retina.		2001	99667
analogue analyser for on-line eye movement detection.	a system of single movement detection is obtained by discriminating the velocities and orientations of eye ball displacements. the orthogonal components of eye velocity (vx and vy) are generated from the horizontal (x) and the vertical (y) components of the movements by means of two derivative circuits and the velocity modulus (v) is generated through a vector operator from vx and vy. the onset and end of individual movements are detected on the basis of a preselected v, vx or vy threshold and, ...	2001	88325
an improved immunoelectrophoretic methodology for the evaluation of protein caloric malnutrition.	an improved technique of two dimensional immunoelectrophoresis of serum proteins on gelatinized cellulose acetate for routine evaluation of protein nutritional state is described. the method, which allows the estimation of more than 30 protein fractions, proves extremely useful in monitoring the effectiveness of total parenteral nutrition and protein metabolic conditions. sera samples from healthy subjects and cachectical neoplastic patients have been examined and preliminary results are reporte ...	2001	92995
clinical experience and expectations with helium and heavy ion irradiation.		2001	96046
[evolution of the free thyroxine index during pregnancy].		2001	95054
severe handicap in the newborn: treatment, non-treatment, or assisted death.		2001	92640
[in memory of sh. m. matevosian].		2001	139545
inhibition of 86rb+ and 22na+ efflux of ehrlich lettré tumor cells by ca2+.		2001	135529
[long-term results of synchronized radiotherapy in inoperable orofacial squamous cell carcinoma].		2001	95490
dental offices as a source of controlled substances.		2001	95243
antilymphocytic serum. (a brief review of current concepts).		2001	95021
peptides with a dual function: central neuroregulators and gut hormones.		2001	95416
effect, on serum lipid levels of omega-3 fatty acids, of ingesting fish-oil concentrate.		2001	92666
[motor complications in patients with parkinson disease treated with levodopa].	motor complications appearing in 180 patients with parkinson's disease who received levodopa for a long period are reviewed. the most frequently observed motor disorders were wearing off fluctuations and dyskinesias of "dose benefit". most patients who presented a decrease in the response to levodopa developed signs during the course or findings in complementary tests which were incompatible with the diagnosis of parkinson's disease. in the present study, the duration of the treatment with levod ...	2001	2631806
can the family physician avoid conflict of interest in the gatekeeper role? an affirmative view.		2000	2723596
[pathways to new antibiotics].		2000	101812
[therapy with beta adrenergic agents].		2000	2869565
notes on the spectrum of opiate use.		2000	97557
characterization of fractional mutations in drosophila: role of rna, protein & repair synthesis.		2000	94307
[effect of phosphoinositides on the structure and permeability of bilayer phospholipid vesicles].		2000	92396
the effect of native and sonicated double-stranded polyribonucleotides on the course of spontaneous autoimmune disease in nzb and nzb/swiss f1 mice.	the administration of sonicated fractions of f2 phage polyribonucleotides caused an increased weight loss and deterioration of the clinical state in female nzb mice. discontinuance of the treatment resulted in an improvement of both the clinical state and the genetically determined autoimmune disorders of these mice. some potential explanations of this effect are discussed.	2000	94768
[the 38th congress of the japan neurosurgical society. abstracts].		2000	94653
debugging the system.		2000	88713
[growth and development of children with cerebral palsy and related nursing action. 4. practice of assistance for the child. 1].		2000	97455
adduct formation between the carcinogen n-acetoxy-2-acetylaminofluorene and synthetic polydeoxyribonucleotides.	the chemical carcinogen n-acetoxy-2-acetylaminofluorene (na-aaf) was reacted with poly(dg-dc) - poly(dg-dc); poly dg - poly dc; poly(da-dt) - poly (da-dt); and poly da - poly dt under a variety of conditions. poly (dg-homo gc polymer and 10--20 more reactive the a + t polymers. lowering the ionic strength increased the extent of reaction, while ph change (8.9 vs. 5.5) had only a small effect. if ionic strength was adjusted so that the two guanine-containing polymers showed equal thermal stabilit ...	2000	18291
[economic crisis in public health in the capitalist countries].		2000	97790
[therapy of cronh's disease in the acute stage, using ornidazole (tiberal). preliminary results].	9 patients with acute exacerbations of crohn's disease were treated over 2.5 months or longer with ornidazole (nitroimidazole). while 3 patients showed no response at all to 5 cycles of ornidazole treatment, 6 patients improved markedly within 2-3 months. in all but 1 patient, the improvement lasted during long-term therapy (average 9 months). therapy was discontinued in 5 of these patients after 2.5-14 months; 2 patients had severe recurrences within 2-10 weeks, while 3 patients remained well d ...	2000	97778
hypertensive complications in patients with aldosterone-producing adenomas.		2000	87647
early detection of cancer of oesophagus along caspian littoral. report of a pilot project.	a simple mass-screening procedure for the early detection by brush cytology of oesophageal cancer in 280 adults in high-incidence villages of the caspian littoral is described. the presence of atypical cells in 7%, and the discovery of frank carcinoma in 3 symptom-free people encourages continuation of the project.	2000	87555
[treatment of pain with psychotropic drugs (author's transl)].	30 patients suffering from therapy-resistant chronic pain were divided into three groups according to drive and mood: i. psychomotor inhibition combined with depressive mood, ii. agitation and depressive mood, iii. agitation combined normal mood. the psychopharmacological treatment of pain showed sufficient results, when the drugs were directed to the target symptoms "agitation", "lack of drive", and "depression". the differentiated administration of activating or sedative antidepressants and ne ...	2000	27829
clinical trial of topical disodium chromoglycate in vernal keratoconjunctivitis.	the clinical picture of spring catarrh was studied in 96 cases. the duration of the disease was found to be 3.5 +/- 2.6 years. it exists all the year round with a higher frequency between april and august. male children were affected about three times more frequently than females. the effect of dscg was studied in 20 cases. it had a significant therapeutic effect. we advise a combined dscg and corticosteroid treatment in the period of exacerbation.	2000	101923
lack of uniformity in colour matching.	1. the fraction of red in a red--green mixture matched to yellow increased as the intensities of the match constituents were increased sufficiently to bleach appreciable chlorolabe and erythrolabe. 2. all changes in matching found for a given normal trichromat, (i) with increase in the intensities of the matching components, (ii) as a function of time after the onset of very intense components, (iii) with change in the pupil region through which light enters the eye, and (iv) with change in the ...	2000	313984
[arrhythmias in direct fetal electrocardiography].		2000	92107
genital herpes zoster: a case resembling primary herpes progenitalis with distal urethral involvement.		2000	101907
[t-lymphocytes in protein-calorie malnutrition in infants].		2000	101962
frontiers of quantitative cytochemistry: a review of recent developments and potentials.	this article attempts to describe the current state of quantitative cytochemical methods and to point out areas where, in the author's opinion, the greatest progress is either being made or can be expected. nearly 300 references to the current literature are cited.	2000	94776
long term survival following firearm injury to the head.		2000	110996
hla antigens in a psoriatic family: comparative studies with mlc, htc, plt and serological hla-dr determinations.	hla genotypes were characterized in a large family of 48 individuals in three generations. in this family, carriers of the proband's disease-predisposing haplotype commonly expressed clinical signs of illness, manifested as psoriasis and/or arthritic lesions. from these data, and from other family studies, presented previously, we have concluded that cutaneous and/or joint lesions may be signs of disease in carriers of the predisposing hla haplotype. we have investigated hla-a, b, c and d/dr ant ...	2000	94702
toward the future in educational systems.		2000	92186
results of cytostatic therapy of metastasizing testicular tumors.		2000	111320
the use of acupuncture needles as sphenoidal electrodes in electroencephalography: a reappraisal.		2000	110550
mesonephric duct remnants (gartner's duct).	in a review of 820 hysterosalpingograms, unilateral opacification of the mesonephric duct (gartner's duct) was observed in five individuals. no associated anomalies of the genitourinary tract or definite evidence of pelvic inflammatory disease was found in any of these patients. the embryology of the genitourinary tract is discussed in some detail, and histologic studies of the uterine cervix are reviewed to explain the radiographic observations.	2000	98997
beta thromboglobulin and glycosylated haemoglobin in diabetes mellitus.	no correlation has been demonstrated between the elevated levels of plasma beta-thromboglobulin and glycosylated haemoglobin found in a group of 97 diabetic patients. the abnormality in diabetic platelets which is reflected by beta-thromboglobulin levels may not therefore be a direct result of poor metabolic control. the highest levels of beta-thromboglobulin were found in those patients with a raised blood urea. glycosylated haemoglobin levels correlated with prevailing blood glucose and with t ...	2000	94458
in vitro analysis of allogeneic lymphocyte interaction. iv. dual recognition of b cell-associated mls locus and i-region determinants by a helper allogeneic effect factor (aef) generated across a minor h locus disparity.	a helper allogeneic effect factor (aef) was produced across an incompatibility at the minor histocompatibility loci. this aef is genetically restricted in its activity since it helps b cells only of the stimulator haplotype and of haplotypes that share both an mls and i-region identity with the stimulator haplotype. the i-region genes involved here map to the i-a and/or i-c subregions. an anti-lym immunoadsorbent column but neither an anti-h-2 nor an anti-ia column absorbed aef helper activity. ...	2000	87470
the production of hydroxyl radical by tallysomycin and copper(ii).		2000	87348
the nursing care and management of coronary artery grafts (revised).		2000	87189
radiation biophysical studies with mammalian cells and a modulated carbon ion beam.		2000	97728
the ultrastructure of cell division in euglena gracilis.	the ultrastructure of mitosis in euglena gracilis was investigated. at preprophase the nucleus migrates anteriorly and associates with the basal bodies. flagella and basal bodies replicate at preprophase. cells retain motility throughout division. the reservoir and the prophase nucleus elongate perpendicular to the incipient cleavage furrow. one basal body pair surrounded by a ribosome-free zone is found at each of the nuclear poles. the spindle forms within the intact nuclear envelope- polar fe ...	2000	97303
reduced plasma viscosity among joggers compared with non-joggers.		2000	92344
use of the fluorochrome 4'6-diamidino-2-phenylindole in genetic and developmental studies of chloroplast dna.	use of the dna-specific fluorochrome 4'6-diamidino-2-phenylindole (dapi) makes it possible to examine in situ the structure of chloroplast dna (chdna) with the fluorescence microscope. this simplifies the study of genetic and developmental changes in chloroplast dna. three examples are presented. (a) wild-type euglena gracilis b contains several chloroplast dna nucleoids per chloroplast. a yellow mutant lacking functional chloroplasts is similar, but such nucleoids are absent in an aplastidic mu ...	2000	90051
interaction of alcohol and reward in an achievement situation.	effects of psychological stress on acute alcohol intoxication following intake of 2 ml whisky/kg body weight were examined in 12 healthy subjects. each subject performed a 45-min psychomotor task under four conditions, two of which increased achievement stress by promising the subject a monetary reward for rapid and accurate performance. the conditions were (a) task only, (b) task + reward, (c) task + alcohol, and (d) task + reward + alcohol. in condition (b) reward improved performance and heig ...	2000	111286
biliary duct ultrasound.		2000	99016
colitis cystica profunda.		2000	99009
drug-induced lung disease.	since there are no diagnostic studies to confirm the presence of a drug-induced lung reaction the physician will make a correct diagnosis only if he is aware of the drugs which have been identified to cause pulmonary reactions and their specific manifestations. failure to recognize a drug-induced lung disease can lead to significant morbidity and in some cases mortality. the major drug-induced lung diseases are reviewed, the drugs being presented in the context of their clinical use and the reac ...	2000	102005
deafness due to hypervitaminosis d.		2000	87663
esophagogastrectomy for carcinoma of the middle third of the esophagus.	elective esophagogastrectomy and reconstruction by esophagogastrostomy were performed on 55 patients with malignant tumors of the midesophagus, despite invasion of contiguous structures in 60% and regional lymph node involvement in 75%. the operations were invariably palliative. two patients died within thirty days of operation. dysphagia was relieved and oral alimentation resumed in the other 53. twenty-nine patients who had experienced painful swallowing and 16 who had vomiting obtained relief ...	2000	92220
loin pain/haematuria syndrome.		2000	86752
[a life with psychiatric patients. 8. a patient who wanted to see the blue sky].		2000	97451
detection of specific rnas or specific fragments of dna by fractionation in gels and transfer to diazobenzyloxymethyl paper.		2000	94421
steroids in asthma.		2000	91910
detection of bacterial pathogens by phage antibody display.		2000	11360836
food poisoning outbreak from contaminated fish-balls.	on february 9th, 1998, a food poisoning outbreak occurred at a boarding school for underprivileged students. an unmatched case-control study was done. an environmental survey, laboratory study of rectal swab culture, fish-balls, water and the cooking utensils were also performed. there were 132 suspect cases, of which the attack rate in teachers was 9.8 per cent (4/41), 16.7 per cent (1/6) in the food handlers and 15.7 per cent (127/810) in the students. the median incubation period was 18 hours ...	2000	11215857
characterization of vibrio parahaemolyticus manganese-resistant mutants in reference to the function of the ferric uptake regulatory protein.	in many bacteria, the ferric uptake regulatory protein (fur) has a central role in the negative regulation of genes affected by iron limitation. in this study, vibrio parahaemolyticus strains carrying mutations in the fur gene encoding fur were isolated by the manganese selection method to assess the function of fur in connection with alternations in the coordinate expression of the siderophore vibrioferrin (vf) and iron-repressible outer membrane proteins (iromps). ten out of 25 manganese-resis ...	2000	11220684
pandemic spread of an o3:k6 clone of vibrio parahaemolyticus and emergence of related strains evidenced by arbitrarily primed pcr and toxrs sequence analyses.	vibrio parahaemolyticus o3:k6 strains responsible for the increase in the number of cases of diarrhea in calcutta, india, beginning in february 1996 and those isolated from southeast asian travelers beginning in 1995 were shown to belong to a unique clone characterized by possession of the tdh gene but not the trh gene and by unique arbitrarily primed pcr (ap-pcr) profiles (j. okuda, m. ishibashi, e. hayakawa, t. nishino, y. takeda, a. k. mukhopadhyay, s. garg, s. k. bhattacharya, g. b. nair, an ...	2000	10655349
[acute gastroenteritis caused by urease positive vibrio parahaemolyticus in an immunocompromised patient].		2000	10905019
characterization of vibrio parahaemolyticus isolates obtained from foodborne illness outbreaks during 1992 through 1995 in taiwan.	vibrio parahaemolyticus is an important foodborne pathogen in taiwan and many other asian countries. a total of 371 isolates of v. parahaemolyticus collected from patients involved in foodborne illness outbreaks in taiwan from 1992 to 1995 were characterized. these isolates had typical biochemical characteristics and only 4% were urease positive. the most frequently isolated serovars were o5:k15 (18.5%), o4:k8 (16.2%), o3:k29 (12.5%), o1:k56 (8.3%), o2:k3 (6.5%), and o4:k12 (6.0%). most of the i ...	2000	10914657
analysis of gyrb and toxr gene sequences of vibrio hollisae and development of gyrb- and toxr-targeted pcr methods for isolation of v. hollisae from the environment and its identification.	isolation of vibrio hollisae strains, particularly from the environment, is rare. this may be due, in part, to the difficulty encountered when using conventional biochemical tests to identify the microorganism. in this study, we evaluated whether two particular genes may be useful for the identification of v. hollisae. the two genes are presumed to be conserved among the bacterial species (gyrb) or among the species of the genus vibrio (toxr). a portion of the gyrb sequence of v. hollisae was cl ...	2000	10919814
distribution of vibrio cholerae virulence genes among different vibrio species isolated in sardinia, italy.	the members of the genus vibrio include harmless aquatic strains as well as strains capable of causing epidemics of cholera. diarrhoea caused by vibrio cholerae is attributed to cholerae enterotoxin (ct) codified by the ctx operon and regulated by a number of virulence genes such as toxt, toxr and toxs. fifty-two vibrio strains were isolated from different aquatic environments in and around sardinia and searched by pcr for the presence of ctxa, zot, ace, toxr, toxs, toxt, tcpa and vpi virulence ...	2000	10747228
effect of mg(2+) ion in protein secretion by magnesium-resistant strains of pseudomonas aeruginosa and vibrio parahaemolyticus isolated from the coastal water of haldia port.	a rapidly growing industrial complex including oil refineries and chemical industries has developed around the coastal area of haldia port in the district of midnapore, west bengal, india. the coastal water is highly polluted with industrial wastes along with petroleum hydrocarbons. the bacteria isolated from the different sites of the coastal waters were escherichia coli, alcaligenes, acinetobacter, klebsiella spp., micrococcus spp., vibrio spp., pseudomonas aeruginosa and vibrio parahaemolytic ...	2000	10754240
proteomics on full-length membrane proteins using mass spectrometry.	a general technique has been developed that allows rapid mass spectrometric analysis of full-length membrane proteins [whitelegge, j. p., le coutre, j., et al. (1999) proc. natl. acad. sci. u.s.a. 96, 10695-10698]. using in-line hplc electrospray ionization mass spectrometry (lc-ms), different native and recombinant bacterial membrane proteins of up to 61 kda are characterized. mass spectrometric data of four entirely different membrane proteins from three bacterial organisms, two transporters, ...	2000	10757971
resuscitation of viable but nonculturable cells of vibrio parahaemolyticus induced at low temperature under starvation.	vibrio parahaemolyticus is known to exist in a viable but nonculturable state when incubated at low temperature under starvation. it has long been debated whether the culturable cells which appear after temperature upshift are the result of true resuscitation or regrowth of a few residual culturable cells. starved v. parahaemolyticus cells at 4 degrees c reached the nonculturable stage in about 12 days. the true resuscitation of nonculturable cells of v. parahaemolyticus occurred after spreading ...	2000	10779722
nhaa, an na(+)/h(+) antiporter involved in environmental survival of vibrio cholerae.	vibrio cholerae, the agent of cholera, is a normal inhabitant of aquatic environments, in which it survives under a wide range of conditions of ph and salinity. in this work, we identified the nhaa gene in a wild-type epidemic strain of v. cholerae o1. nhaa encodes a protein of 382 amino acids that is very similar to the proteins nhaa of vibrio parahaemolyticus, vibrio alginolyticus ( approximately 87% identity), and escherichia coli (56% identity). v. cholerae nhaa complements an e. coli nhaa m ...	2000	10781565
the marine pathogen vibrio vulnificus encodes a putative homologue of the vibrio harveyi regulatory gene, luxr: a genetic and phylogenetic comparison.	vibrio vulnificus is an opportunistic pathogen that exhibits numerous virulence factors, including the secretion of a zinc metalloprotease and the production of a capsule. we have cloned and sequenced a gene from v. vulnificus that is a homologue of the positive transcriptional regulator, luxr, of the lux operon in vibrio harveyi. this gene encodes a putative, single complete open reading frame designated smcr, which shares greater than 75% nucleotide identity with luxr of v. harveyi. the deduce ...	2000	10806366
application of pulsed-field gel electrophoresis to the investigation of a nosocomial outbreak of vibrio parahaemolyticus.	the method of pulsed-field gel electrophoresis (pfge) for vibrio parahaemolyticus was first published in 1996. since then, its application has been seldom reported in the literature. a food poisoning outbreak due to v. parahaemolyticus occurred in five wards of a hospital was investigated using this method. twenty-five patients were involved and all of them had eaten food supplied by the hospital. of the 15 cases whose stools were available for culture, only four cultures yielded v. parahaemolyt ...	2000	10806961
enterotoxicity and cytotoxicity of vibrio parahaemolyticus thermostable direct hemolysin in in vitro systems.	vibrio parahaemolyticus is a marine bacterium known to be a common cause of seafood gastroenteritis worldwide. the thermostable direct hemolysin (tdh) has been proposed to be a major virulence factor of v. parahaemolyticus. tdh causes intestinal fluid secretion as well as cytotoxicity in a variety of cell types. in this study, we investigated the interplay between the hemolysin's enterotoxic and cytotoxic effects by using both human and rat cell monolayers. as revealed by microspectrofluorimetry ...	2000	10816461
vibrio parahaemolyticus infections in the united states, 1973-1998.	vibrio parahaemolyticus infections are associated with consumption of raw or undercooked shellfish, contaminated food, and exposure of wounds to warm seawater. foodborne outbreaks and sporadic infections from vibrio species in 4 gulf coast states are reported routinely to the centers for disease control and prevention (cdc). between 1988 and 1997, 345 sporadic v. parahaemolyticus infections were reported: 59% were gastroenteritis, 34% were wound infections, 5% were septicemia, and 2% were from o ...	2000	10823766
isolation of a pandemic o3:k6 clone of a vibrio parahaemolyticus strain from environmental and clinical sources in thailand.	application of an immunomagnetic enrichment method selective for vibrio parahaemolyticus serovar k6 allowed isolation of a strain belonging to the pandemic o3:k6 clone of v. parahaemolyticus from fresh shellfish not implicated in a clinical case in southern thailand. arbitrarily primed pcr profiles of this strain, clinical o3:k6 strains isolated from sporadic diarrhea cases in the same area, and a standard pandemic o3:k6 strain were indistinguishable.	2000	10831459
chemical and serological properties of lipopolysaccharides from vibrio parahaemolyticus o-untypeable strains isolated from patients.	chemical and serological studies have been carried out on the o-antigenic lipopolysaccharides (lps) of six strains, u-6443, w-90144, x-3972, ad-7999, 90a-6611 and kx-v212, of vibrio parahaemolyticus isolated from patients. the o-serotypes of these strains have not been identified because they were not agglutinated by any diagnostic antisera against known o-serotype strains. a compositional sugar analysis of their lps revealed that out of the six o-untypeable (out) strains, u-6443, w-90144 and ad ...	2000	10832965
a filamentous phage associated with recent pandemic vibrio parahaemolyticus o3:k6 strains.	a specific serotype, o3:k6, of vibrio parahaemolyticus has recently been causing epidemics of gastroenteritis in southeast asia, japan, and north america. to examine whether the new o3:k6 strains possess characteristics that may exacerbate outbreaks, we compared v. parahaemolyticus o3:k6 strains with non-o3:k6 strains using strains isolated from individuals with traveler's diarrhea at kansai airport quarantine station, osaka, japan. all 24 o3:k6 strains possessed a common plasmid, po3k6 (dna siz ...	2000	10834969
molecular characterization of vibrio parahaemolyticus vsglt: a model for sodium-coupled sugar cotransporters.	the na(+)/galactose cotransporter (vsglt) of vibrio parahaemolyticus, tagged with c-terminal hexahistidine, has been purified to apparent homogeneity by ni(2+) affinity chromatography and gel filtration. resequencing the vsglt gene identified an important correction: the n terminus constitutes an additional 13 functionally essential residues. the mass of his-tagged vsglt expressed under its native promoter, as determined by electrospray ionization-mass spectrometry (esi-ms), verifies these 13 re ...	2000	10835424
analysis of the polar flagellar gene system of vibrio parahaemolyticus.	vibrio parahaemolyticus has dual flagellar systems adapted for locomotion under different circumstances. a single, sheathed polar flagellum propels the swimmer cell in liquid environments. numerous unsheathed lateral flagella move the swarmer cell over surfaces. the polar flagellum is produced continuously, whereas the synthesis of lateral flagella is induced under conditions that impede the function of the polar flagellum, e.g., in viscous environments or on surfaces. thus, the organism possess ...	2000	10850984
antibacterial activity of a chitooligosaccharide mixture prepared by cellulase digestion of shrimp chitosan and its application to milk preservation.	the antibacterial activity of a chitooligosaccharide mixture prepared by digestion of shrimp chitosan with cellulase at 50 degrees c for 14 h was evaluated. sugars with 1 to 8 degrees of polymer (dp) were found in this chitooligosaccharide mixture, and the weight percentage of sugars with dp > or = 6 was 44.3%. minimal lethal concentrations of this mixture against aeromonas hydrophila, escherichia coli, listeria monocytogenes, pseudomonas aeruginosa, salmonella typhimurium, shigella dysenteriae, ...	2000	10852568
characterization of the vibrio parahaemolyticus na+/glucose cotransporter. a bacterial member of the sodium/glucose transporter (sglt) family.	the vibrio parahaemolyticus sodium/glucose transporter (vsglt) is a bacterial member of the sglt gene family. wild-type and mutant vsglt proteins were expressed in escherichia coli, and transport activity was measured in intact cells and plasma membrane vesicles. two cysteine-less vsglt proteins exhibited sugar transport rates comparable with that of the wild-type protein. six residues in two regions of vsglt known to be of functional importance in sglt1 were replaced individually with cysteine ...	2000	10852908
the use of colony hybridization in the isolation of thermostable direct hemolysin-producing vibrio parahaemolyticus from foods implicated in an incidence of food poisoning.		2000	10871921